Materials and methods –
several materials, dyes, and media were used for identification purposes. List them separately. In Materials and Methods, you explain what the test is and what you use. In the results, you explain why you did this and what the results are. (For example, in M&M, you describe a Gram stain, the dyes, how the stain works, then in the results, you explain that your microbe was gram (_____) because the bacteria appeared (this color).
Discussion – based on your observations of the results, draw your conclusions. Provide a description of your microbe. Include the following in your description:
Group 3 patient
Patient presentation: severe headache, sensitivity to light, torticollis x 2 days. Temp 102.
A spinal tap sample was collected. The following tests were performed with the results listed.
Using the information given to you, along with the information you have gathered over the past few weeks in lab, you should be able to determine what microbe (it will be a bacterial infection) is causing this infection.
test |
result |
appearance |
||
Methylene blue |
+ for bacteria |
cocci |
||
Gram stain |
– |
|||
catalase |
Positive or negative? |
Bubbles formed |
||
oxidase |
purple |
|||
Capsular stain |
+ | |||
flagella |
||||
endospore |
||||
plate |
||||
EMB plate |
No growth |
|||
MacConkey plate |
Large, smooth, pink colonies |
|||
Chocolate agar |
Growth |
|||
SBA plate |
Clear |
|||
MSA plate |
||||
Starch plate |
Halo after addition of iodine |
The microbe is most likely _____________________________ (Genus species)
Pierre,1 |
Goddeene Pierre
Professor Leslie Wooten
MCB2004
February 25,2021
Unknown ID Lab Report
The patient assigned to my group suffered from migraines, slight sensitivity, torticollis- which contractions of the neck muscle, and a high temperature of 102, and was therefore performed a spinal tap. A spinal tap involves a needle being inserted between two lumbar vertebrae bones to collect cerebrospinal fluid. The results of the test indicated that our patients suffered from meningitis which is caused by a microbe known as Neisseria meningitidis. The full taxonomic name for this microbe is Bacteria Bacteria Proteobacteria Betaproteobacteria Neisseriales Neisseriaceae Neisseria meningitidis.
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