Experimental Design Assignment

You were given an Aedes aegypti gene of unknown function. Using Blast you were able to find the homologs of your gene. You have done research regarding the function of the homologs. Using this information:

A.Construct a hypothesis

Give a hypothesis on the function of your gene SHAKER is in Aedesaegypti.

B.Design an experiment to test your hypothesis.

Include a labeled sketch and written summary of experiment. (include drawing of all conditions, negative/positive etc)

C. Variables 

List the Dependent and Independent
List Control variable
List a Positive and /or Negative controls

D. Create a data set and figure

Create a graph that clearly conveys to the reader what your experiment is about.

F.Interpretation

Give an interpretation of the possible meaning of your data. (although this isn’t conclusive since we are not doing statistics) . Does it align with your hypothesis?

G.Self-critique and follow-up questions:

Why might your conclusion be wrong, what other questions do you have.

1

My Gene Shaker

Student Name

Institution Affiliation

Date

My Gene Is Shaker

The operation of the potassium ion channels that are integral membrane in the proteins has an important part that is played by the shaker gene. They play a crucial part in the correct cell functioning. A common ancestor has genes that can be inherited by two species and the genes are called homologs. The function of the homologs is to ensure that cells are functioning correctly (Mysore et al., 2020). When a genetic screening was done, it resulted in different mutants that showed the same type of phenotype.

The nucleotides sequence in the DNA carry the genetic information of the biological system. Each DNA is formed from double complementary strands of nucleotides in this biological system. Hydrogen bonds G-C are what keeps the strands of the DNA together (DiCarlo, Mahajan & Tsang, 2018). The eucaryotes are kept in the nucleus of the cell and they are kept in pairs. Better vision and hearing abilities are what are associated with my gene. The strands of the DNA that they are associated keep the memory of the gene. As far as the gene has not mutated, the shaker gene of the multiple disease vector mosquitoes would determine their actions.

In order to determine if the phenotype is the same, a large-scale screening has to be done. A full complementation test can be done to reveal that the genes fall in the same biological system, if they are recessive (Papazian et al, 2017). In order to show how the gene behaves in the biological system, careful characterization of the phenotype can be done.

Conclusion

The shaker gene play a crucial part in the correct functioning of the cell and the shaker gene can be inherited by two species of a common ancestor. These inherited genes are called homologs. The biological system carries the nucleotides sequence in the DNA and they are kept together by the hydrogen bonds G-C. The memory of the gene is kept in the strands of the DNA that are associated with the gene memory. A large screening has to be done to determine if the phenotypes are the same and a complementation test can be done to show if the genes fall in the same biological system.

References

DiCarlo, J. E., Mahajan, V. B., & Tsang, S. H. (2018). Gene therapy and genome surgery in the retina. The Journal of clinical investigation, 128(6), 2177-2188.

Mysore, K., Hapairai, L. K., Sun, L., Li, P., Wang, C. W., Scheel, N. D., … & Duman-Scheel, M. (2020). Characterization of a dual-action adulticidal and larvicidal interfering RNA pesticide targeting the Shaker gene of multiple disease vector mosquitoes. PLoS neglected tropical diseases, 14(7), e0008479.

Papazian, D. M., Schwarz, T. L., Tempel, B. L., Jan, Y. N., & Jan, L. Y. (2017). Cloning of genomic and complementary DNA from Shaker, a putative potassium channel gene from Drosophila. Science, 237(4816), 749-753.