C
O
o!
ui
U
t-o
–co+
ior;
Uro:
t–u
n ;:
i”a .:
PF
g
F
e
e
rJ
UT
Fl
B
G
Itr
(
a
+9
i’-i ‘r !
9dr : c
-o
I
o’tr
u
CL
eee
s$sxs
B* t $ 3
E ^^ =l)ii36te
c,5
s@
C)- a
0,
OQ
trc
SF
,; .G
or 0
o
l-\
{S
4
=d(o
€.\
Ar$E{g
={Poi\r
f
15
oJ il.xa
=d 4^\a .-sVEr.! v:oir i,1!ts 0
o-ii o
r-9 p
-5->s oii i.: u
00
E
I
C
o.
c
L
z
+It +
I
3
j
€J
0+
l;
Or
no.f,
Yq
(X!
00
?o
_q
E
$g
E!,t
E5a- ati€
Eru
Srfilf
ttt-,
\l=/
t
fu
t\i
o
\,
+rh
$$
sE3
L
^^4lr
-) !+’.- .;l;’,
\7
trLt!
++”
Mixed Unknown – Day One
Gram +
Gram –
Gram +
Gram –
Columbia CNA Agar
MacConkey’s Agar
Mixed Unknown Identification Form
Unknown “Patient Specimen” Identification #:
Student’s Name:
For Gram Positive Bacteria Identification
First Test Media Requested (1 point)
1.
2.
Second Test Media Requested (4 points)
1.
2.
3.
4.
5.
Unique Characteristics & Test Results (15 points)
Gram Stain Reaction (5 points):
Cell Shape (5 points):
Cell Arrangement (5 points):
Tests & Results (20 points)
Tests Results
1.
2.
3.
4.
5.
Name of Gram-Positive Bacterium (Genus & species-10 points):
For Gram Negative Bacteria Identification
First Test Media Requested (1 point)
1.
2.
Second Test Media Requested (4 points)
1.
2.
3.
4.
5.
Unique Characteristics & Test Results (15 points)
Gram Stain Reaction (5 points):
Cell Shape (5 points):
Cell Arrangement (5 points):
Tests & Results (20 points)
Tests Results
1.
2.
3.
4.
5.
Name of Gram-Negative Bacterium (Genus & species-10 points):
BIO2020 Microbiology Spring 2021
Mixed Unknown Instruction
Directions:
The mixed unknown exercise has been modified for the remote lab. You will be receiving
image files for your Unknown Day 1 by Monday, April 12th in the black board (Course
Content). This file has images including Gram stains and isolated colonies (one on MacConkey’s
and one on Columbia CNA). You are to use this information to request additional tests that you
need to perform. I’ll send you the images of your requests by email.
You will have another sub-folder, where you will find the Instruction, Mixed Unknown
Identification Form and Mixed Unknown Identification Sample for Writing the Result.
You will have TWO TEST REQUESTS. Your FIRST MEDIA TEST REQUEST IS DUE NO LATER THAN
WEDNESDAY, the April 14th by midnight. Once submitted, I will send your results from your
selected tests results by Friday, April 16th by midnight.
Your SECOND MEDIA TEST REQUEST IS DUE NO LATER THAN MONDAY, April 19th by midnight.
Once submitted, I will send you results from your selected tests results by Friday, April 23rd by
midnight. Please keep in mind that I am doing this for every student in your section.
Your FINAL COMPLETED WORKSHEET is DUE ON MONDAY, April 26th by midnight. Please use
the correct name of the test requested (i.e. Skim Milk agar, not casein, Starch Agar, not amylase
test etc.).
Please get the Mixed Unknown Identification Form, attached with this email. On the day that
the assignment is due, you will need to have all of the information on this sheet filled out. Any
portion not filled out when you turn it in, will result in no points for that section. You must
include the genus and species of each organism, spelled out completely, underlined or
italicized, in order to get full credit. You must scan or take picture and send me by email the
Completed Mixed Unknown Identification Form by April 26th by midnight. You will get a
deduction of 10 points for every day of late submission until April 30th midnight. You will get
zero after submitting April 30th.
Important Points
1. You are given one Gram-positive and one Gram-negative bacterial images. You must
identify both the bacteria.
2. Mac Plate: Either fermenter or non-fermenter
3. CNA Plate: Hemolysis may be shown with light reflection as shown during the lab
4. You can request test results twice.
5. Test Request must be made by writing on the “Mixed Unknown ID Form”. That means,
you must write the request in the ID Form and send to me as attachment. Please see
below.
6. Request must be logical (Will get 5 points deduction if not logical). I already explained
that during your lab on Wednesday (April 7th).
7. Follow binomial nomenclature.
8. You must follow the Mixed Unknown Flow Chart that I have provided in the black
board. Tests results outside of the flow chart will not be provided. I’ll deduct 5 points if
you request test results outside of the flow chart (logically). As for an example: let’s say
you request IMVC test for your gram-positive bacterium. I’ll give you -5 and tell you
that the test you have requested is not applicable test for that specific bacterium.
First Request
Second Request
9. Always refer to the flowchart before requesting media. Some media cannot be
requested until certain results are available. Otherwise, you may order multiple media
simultaneously.
10. You must send your requests and final submission by email.
11. You must write “Mixed Unknown-Sample number-Your name-Request 1” (As for
example: Mixed Unknown-800-Paul Gomez-Request 1) on the subject heading of every
email. You will get 2 points deduction for not writing the proper heading in the email.
Please see below.
Mixed Unknown-800-Paul Gomez-Request 1
Mixed Unknown-800-Paul Gomez-Request 2
Mixed Unknown-800-Paul Gomez-Final Submission
12. You must write a formal letter in the mail if you write regarding your mixed unknown.
You will get 2 points deduction for not writing the email properly. As for example:
Dear Dr. Gomez:
………………………………………………………………………..
………………………………………………………………………..
Your name
Check the following before sending me “Request Email”
1. Did you write correct subject heading (otherwise -2)?
2. Is your test logical following the flow chart (otherwise -5)?
3. Did you write any email to me regarding mixed unknown? You must write my name and
your name (otherwise -2)?
4. Did you write the request in the MIXED Unknown ID Form (if not -2)?
5. Late request-1 or 2 for each day (-5).
6. More than one request email for “EACH REQUEST” (-5 for additional email)
7. Late for Final Submission for each day (-5) until April 30th.
8. Late for Final Submission after April 30th (-100). You will get zero.
9. There will be no extension of date except real emergency (with document) or
acceptable excuses.
I’ll post Image of Unknown Samples, Instruction, ID Form, Sample ID Form April 12
First Request from Student by: April 14
I’ll send Images of the Results of Tests of First Request you asked for: April 16
Second Request from Student by: April 19
I’ll send Images of the Results of Tests of Second Request you asked for: April 23
Students Submit Final Completed ID Form by: April 26
Check the following before sending me “Final Submission”
1. Did you write correct subject heading (otherwise -2)?
2. Did you write any email to me regarding mixed unknown? You must write my name and
your name (otherwise -2)?
3. Confirm your writing in the “Mixed Unknown Identification Form” following the “Mixed
Unknown Identification Form Answering Sample.”
