Topic: Oklahoma has one of the lowest fruit & vegetable consumption rates in the US. In what ways could someone increase their consumption and why are these carbohydrate sources important? (Simple vs Complex carbohydrates, Micronutrients)
Don't use plagiarized sources. Get Your Custom Essay on
HISTORY or Background Information – see slides 4-8 for suggested slides
a. 4 facts with appropriate APA in-text citations (matched to full citations on the REFERENCES slide at the end of the slideshow.
b. Minimum of one photo with appropriate APA in-text citation (matched to full citation on the REFERENCES slide at the end of the slideshow.
FOOD SCIENCE – see slides 10-16
a. 4 terms with definitions
b. 3 Food Science Facts – each fact has 3-4 details (and APA in-text citations matched to full citations on the REFERENCES slide at the end of the slideshow).
c. Minimum of one photo with appropriate APA in-text citation (matched to full citation on the REFERENCES slide at the end of the slideshow.
TECHNIQUE, PROCESS, OR CULINARY METHOD OF PREPARATION – see slides 17-21
a. 3 terms with definitions
b. 3 cultural origins of food/culinary technique
c. 2 photos or videos of a food that uses the defined culinary technique above
NUTRITIONAL OR SENSORY IMPLICATIONS (NSI) – see slides 22-27
a. complete descriptions on slide 23
b. 2 NSI facts with 3-4 details and APA in-text citations
c. nutrient measures as on slide 25
KEY LEARNING—see slides 28-31
a. Summarize most important information from
1. Food Science
2. Culinary Method
3. Nutritional/Sensory Implications
b. explain how your presentation answers the question assigned
c. give 3 examples of how the presentation can be used
REFERENCES –see slide 32, use APA full citations
a. cite all texts, books, and academic journals
b. cite websites
c. cite video documentaries and other videos
d. cite photos
e. keep slide 33 as it gives credit to the template creators
Rubric is attached below. and the sample slide is also attached below.
Need around 10-15 slides.
Fats and oils
Ways someone could choose Healthier fats to cook and eat.
Cooking technique depends on the oil ,fats, and heat. Oils and fast production.
TABLE OF CONTENTS
Lipids,
Lard , margarine , shortening , cooking spray olive oil,
Technique, process, or culinary method of preparation
Oil and fats food science:
02
03
Introduction
01
04
Nutritional or Sensory implications
taste
(Fabflour,2020)
History or Background Info
Fats and oils are lipids , Lipids—comprehensive term including fats, oils, and
some other organic compounds containing carbon and
hydrogen and only a very small amount of oxygen.
Chemical Explanation:
01
For optimal health people need to avoid consuming too much fats because association with overweight and heart disease particulary saturated fats (cousimenr 2016).fats rendered from hogs. Contains no water. Used
in cooking for centuries. Distinctive flavor, but becomes
rancid and needs to be refrigerated.
History
(Bob, 2020)
Fats obtain from hogs consider to be the prime lard
Leaf lard
A BRIEF STORY — fats and oil discoveries
Very clarified butter from which the water and milk solid have been removed by heating and filtering used in indian recepy
ghee
Occasionally called oleomargarine originally developed from vegetable products. , oils from plant , corn soybean oil,sunflower canola etc
Also one of the earliest method of oil processing is hydrogenation..
margarines
This is a diagram of lipids
MILESTONES REACHED in science or culinary technique
01
Mercury is the smallest planet in our Solar System
MERCURY
03
Despite being red, Mars is actually a cold place
MARS
02
Venus has a beautiful name, but it’s terribly hot
VENUS
Food Science
Different food products have different requirements as far as the functionality of the fat they contain is concerned.
02
Definition
Fat render from hogs.The prime lard is called leaf lard Contains no water. Used
in cooking for centuries. Distinctive flavor, but becomes
rancid and needs to be refrigerated.
(cousimenr , 2016. Pg 154).
lard
Food Science terms of fats oil
Definition
made from the milk of many animals. Contains
about 16% water. Popular.
(cusminer, 2016. Pg 154).
Butter
contuniuees
Definition
produced from vegetable oils but has the
characteristics of butter. Hydrogenation of unsaturated
fatty acids causes trans fatty acids (possibly raising the
risk of coronary heart disease)
(cusminer, 2016. Pg 154).
margrines
contuniuees
Definition
Spreads—contain less calories than margarine
as whipped air increases the volume
.
(cousminer, 2016. Pg 154).
Whipped spread
contuniuees
Definition
Shortenings—the first cousins of margarine. Designed
for baking, do not contain water, do not become rancid.
.
(cusminer, 2016. Pg 154).
shortening
contuniuees
Definition
Spray—a convenience product that adds almost
no calories..
(cusminer, 2016. Pg 154) (dr.tessman pp).
cooking spray
contuniuees
Food Science Fact
The fast of commercial interest form animals are milk fat, lard,tallow cousminer 2016).
Steps of production of lard or tallow by rendering , refingning , hydrogenation,blending and tempering, winterizing.
Origins of fats
Fats vary in natural characteristic, depending upon their origin(cousimner 2016)
Some change in fatty acid comsition of non ominant can be accomplished by mdifiying the diet.
There are many different choices of cooking fat, and most of us use them to cook our food from time to time.
— By Michael Joseph 2018, 25 Types of Cooking Fats and Oils: Nutrition Facts, Benefits, Drawbacks, https://www.nutritionadvance.com/types-of-cooking-fats-and-oils/
Rendering
The first step of producing lard or tallow is extraction of fat from tissues
Refingng
Fats and oil rresulting from rending or extraction.
During this process only one chemical reaction occurs.
hydrogenation
process of adding hydrogen to polyunsaturated fatty acid to change oil into solid fats.
Blending and temparing
Oils can be blended wih soldi fats to produce fats with excellent spreading an screaming characterist over a wide temperature range.
winterizing
Process of chilling oil to 45 f(7.2c)and then filtering it to remove any fats cystall.
Oils and fats production
03
Tan Sri Datuk Dr ,2015.
Cultural Origins of food/culinary techniques
TRANS FAT
Germany
CRISCO
England
ghee
india
01
03
02
The technique in preparing food
Frying: a medium utilize in bothy shallow fat – need good heat control and deep fat frying. -usually used thermometer
And also oil and fats give color and flavor and aroma all aspect of palatability that can be influence by the type fat select
Two textural qualities and tenderness and flakiness are influenced by fats.
01
03
02
Nutritional or Sensory implications
04
What senses (hear, see, taste, touch, and smell) are involved in the culinary process, or the scientific reactions?
Taste
Fats and oils add pleasing sensory qualities to broad range of food but they also concerned about calories most people avoid consuming too much.
sensory
Key Learning
Most important information , Lipids provide the most concreted source of energy of the food solids
Key Learning from the Presentation
Culinary Method
Hydrogenation Rendering
Nutr/Sensory Implications
Food Science
unsaturated fats — Monounsaturated and polyunsaturated fats — lower disease risk. Foods high in good fats include vegetable oils (such as olive, canola, sunflower, soy, and corn), nuts, seeds, and fish.
trans fats — increase disease risk, even when eaten in small quantities. Foods containing trans fats are primarily in processed foods made with trans fat from partially hydrogenated oil. Fortunately, trans fats have been eliminated from many of these foods.
How does your presentation answer the original question?( from slide 2)
Many fats and oils available in markets have undergone processing steps design to meet federal requirement and consumer desire for a healthful function products
History
01
02
04
03
The function of fat or oil in food product determined by the type of oils or fats used.
Culinary Method
matter exist as solid ,liquid or gas but many foods are combination of two different phase mater.
Liquids composed of carbon ,oxygen and hydrogen but most if the carbon atoms in lipids are bound only to other carbon atoms and hydrogen
Food Science
from the question above in order for people to stay healthy and active is by avoiding consuming too much fat.usually unsaturated fats are healthier
Nutrition
REFERENCES — USE APA style
TEXTS/BOOKS/JOURNALS
Cousminer, J.J. (2017). Culinology: The intersection of culinary art and food science. Wiley. textbook
McWilliams, M. (2013) Food fundamentals, 10th Ed. Pearson. textbook
THANKS
Do you have any questions?
yoeeyoe@email.ecok.edu
(Hands in.., nd.)
CREDITS: This presentation template was created by Slidesgo, including icons by Flaticon, and infographics & images by Freepik
Exceeds Expectations
Meets Expectations
Progressing
Needs Improvement
Sequencing of Information
Points:
4 (4.00%)
Information is organized in a clear, logical way. It is easy to anticipate the type of material that might be on the next slide/page.
Points:
3 (3.00%)
Most information is organized in a clear, logical way. One slide or item of information seems out of place.
Points:
2 (2.00%)
Some information is logically sequenced. An occasional slide or item of information seems out of place.
Points:
1 (1.00%)
There is no clear plan for the organization of information.
Content Quality
Points:
12 (12.00%)
All information provided is accurate and detailed. There is ample content specifics in this presentation to learn about the topics, sources are cited in APA style. Textbook learning is integrated into presentation
Points:
9 (9.00%)
Almost all the information provided is accurate. Some details are provided. Or all the information is accurate but not all of the requirements of the assignment were met. Information is not complete. Little textbook learning evident.
Points:
6 (6.00%)
Few details support main idea. Most information is accurate and almost all of the requirements have been met. Little information on the presentation. No textbook learning evident in the presentation.
Points:
3 (3.00%)
There are several inaccuracies in the content OR many of the requirements were not met. Not enough content, incomplete.
History Section Requirements
Points:
12 (12.00%)
All requirements are present 4 facts with appropriate APA in-text citations
Points:
9 (9.00%)
Most requirements are present (more than half, but not all)
Points:
6 (6.00%)
Half or fewer requirements are present
Points:
3 (3.00%)
Many of requirements are missing
Food Science Info Requirements
Points:
12 (12.00%)
All requirements are present a. 4 terms with definitions b. 3 Food Science Facts – each fact has 3-4 details
Points:
9 (9.00%)
Most requirements are present (more than half, but not all)
Points:
6 (6.00%)
Half or fewer requirements are present
Points:
3 (3.00%)
Many of requirements are missing
Technique, Process, Method Requirements
Points:
12 (12.00%)
All requirements are present a. 3 terms with definitions b. 3 cultural origins of food/culinary technique
Points:
9 (9.00%)
Most requirements are present (more than half, but not all)
Points:
6 (6.00%)
Half or fewer requirements are present
Points:
3 (3.00%)
Many of requirements are missing
Nutr/Sensory Info Requirements
Points:
12 (12.00%)
All requirements are present a. complete descriptions on slide 23 b. 2 NSI facts with 3-4 details and APA in-text citations c. nutrient measures as on slide 25
Points:
9 (9.00%)
Most requirements are present (more than half, but not all)
Points:
6 (6.00%)
Half or fewer requirements are present
Points:
3 (3.00%)
Many of requirements are missing
Key Learning Requirements
Points:
12 (12.00%)
All requirements are present a. summarize most important information from 1. Food Science 2. Culinary Method 3. Nutritional/Sensory Implications b. explain how your presentation answers the question assigned c. give 3 examples of how the presentation can be used
Points:
9 (9.00%)
Most requirements are present (more than half, but not all)
Points:
6 (6.00%)
Half or fewer requirements are present
Points:
3 (3.00%)
Many of requirements are missing
Spelling and Grammar and References
Points:
12 (12.00%)
Presentation has no misspellings or grammatical errors. All sources (information and graphics) are accurately documented in the APA format.
Points:
9 (9.00%)
Presentation has 1-2 misspellings, but no grammatical errors. All sources (information and graphics) are accurately documented, but one is not in APA format.
Points:
6 (6.00%)
1-2 grammatical errors but no misspellings. All sources (information and graphics) are accurately documented, but two or more are not in APA format.
Points:
3 (3.00%)
Presentation has more than 2 grammatical and/or spelling errors. Some sources are not accurately documented.
Photos
Points:
12 (12.00%)
All photos are attractive (size and colors) and support the theme/content of the presentation. All are cited.
Points:
9 (9.00%)
A few photos are not attractive but all support the theme/content of the presentation. Most are cited.
Points:
6 (6.00%)
All photos are attractive but a few do not seem to support the theme/content of the presentation. A few photos are cited.
Points:
3 (3.00%)
Several photos pixelated, or are unattractive or detract from the content of the presentation. Not cited.
fack.indd 14 1/14/2016 8:45:41 AM
CULINOLOGY
®
T H E I N T E R S E C T I O N O F C U L I N A RY A RT A N D F O O D S C I E N C E
RESEARCH CHEFS ASSOCIATION
E D I TO R : J. J E F F R E Y C O U S M I N E R
ftitle.indd 1 1/14/2016 7:59:25 AM
“The mediocre teacher tells. The good teacher explains. The superior teacher demonstrates.
The great teacher inspires.”
— William Arthur Ward
We dedicate this book in memory of our inspirational predecessors:
Gary Holleman
William “Pops” Hahne
Michele Block
John Matchuk, CRC
Craig “Skip” Julius, CRC, CCS, CEC, CCP, CFE
for their passion and dedication to education in the field of food research and development, and their
legacy of commitment to ensuring that Culinology® will continue to grow and thrive.
fdedication.indd 3 1/14/2016 8:50:28 AM
iv • CULINOLOGY®
When I started my career in the food and beverage industry
in the 70s, I could not have imagined life as a research chef,
food scientist, or manufacturer. Those options just did not
exist in my world. And the term Culinology® would not be
coined for several decades.
I backed into the field of Culinology® with a phone call
from Chef Gary Barnette of the newly opened Casino Magic
Hotel and Gaming Resort on the Gulf Coast of Mississippi.
Gary was faced with a flood of hungry gamers all looking for
regional favorites such as gumbo, jambalaya, and crawfish
etouffée. With a cadre of chefs on the team, I was perplexed
as to what Gary might need from me. He explained that his
talented chefs hailed from Vegas and Atlantic City; none were
familiar with Gulf Coast cuisine. That’s where I came in. Gary
needed Cajun and Creole food, and he wanted it yesterday.
Being a businessman and needing a little extra money, I told
him I would cook whatever he needed. Only then did I learn
that he needed 600 gallons of each every four days.
I was a restaurateur and a fine dining chef, not a product
development specialist. Everything I cooked came out of a
sauté pan. My largest piece of cooking equipment was a five‐
gallon Groen kettle. But he wanted 1,800 pounds of multiple
products every few days! And that’s when I learned about
food manufacturing as I was thrust into the emerging field of
Culinology®.
I was quickly introduced to local packers, and as things
rolled along I began to develop 1,000‐pound capacity, auger‐
driven kettles of cast iron. With direct heat to the metal,
I could create authentic and traditional dark‐and light‐brown
rouxs, which set us apart from other manufacturers. Before
long, word spread about my little food manufacturing plant,
and that’s when Chef Tim Soufan of TGI Friday’s called to see
if I might consider working with him to create a special bar-
becue glaze for a Friday’s menu promotion. I simply could not
say no, and, with Chef Soufan’s guidance, Jack Daniel’s Glaze
was born. That product ultimately built and solidified Chef
John Folse & Company Manufacturing.
Realizing that I was now in product development, it was
time to visit the chef oracle for advice and guidance. It was on
the apron strings of world‐renowned culinarian Warren
Leruth, owner of LeRuth’s fine dining in Gretna, that I first
learned the terms food science, R & D Chef, modified food
starch, and flavor bases. Chef Leruth had created many of the
foods and flavors for Popeyes, including their famous bis-
cuits. He had worked with the L.J. Minor Corporation, Nestlé,
and Outback Steakhouse among numerous others. At this
time, most food products for volume feeding were created by
food scientists. For the most part, chefs had not yet thrown
their toques into the arena. I was mesmerized by Chef Leruth,
who was a genius and a phenomenal storyteller.
He talked about Nicolas Appert, who answered Napoleon
Bonaparte’s challenge to create transportable, quality food
for troops on the move. Chef Leruth talked about Louis
Pasteur, the chemist and microbiologist, who developed the
principles of pasteurization during the mid‐1800s. He talked
about John Mason, the Philadelphia tinsmith who invented
and patented the Mason jar; Henri Nestlé, a pharmacist who
helped develop condensed milk; Henry Heinz, founder of H. J.
Heinz Company; and Clarence Birdseye, founder of the
modern frozen food industry. We discussed names like J.L.
Kraft, who revolutionized cheese production, and John
Dorrance, who devised a method to condense soup and
eventually bought out the Campbell’s Soup Company. We
even discovered we had a mutual friend, Louis Szathmary,
who in just 10 years went from immigrant short‐order cook
to developer of frozen food products for Stouffer’s and, later,
NASA. As a lover of history, I was enthralled and found his
document about stocks and coagulation by Marie‐Antoine
Carême fascinating.
As I got up to leave, Chef Leruth jotted a quick note, then
sealed it in an envelope inscribed with my name. It was a gift
that I was not to open until he was gone. Many years later,
after eulogizing Chef Leruth at his funeral in November 2001,
I opened the envelope to discover his secret recipe for his
magnificent golden vanilla ice cream, which he had created
as the base for his Chelsey’s Frozen Custard company. It is
one of the greatest gifts I have ever received. When I left Chef
Leruth’s company, my brain was spinning with thoughts and
possibilities. That is how I spent my first day of school in
volume food manufacturing.
In 1995, I acquired a small USDA food manufacturing
plant in New Orleans. As I became more involved in food
manufacturing, I met icons such as Ferdinand Metz of H. J.
Heinz; Juerg Aeschbach, product developer for Albertsons
and Wolfgang Puck Food Company; and John and Betsy
Lattanza, who were also mentors. At that time, chefs really
Foreword to Culinology®:
The Intersection of Culinary
Art and Food Science
ffore.indd 4 1/14/2016 8:49:41 AM
had few resources to learn about the science of cooking.
Thank goodness we did have Harold McGee’s On Food and
Cooking, which was published in 1984.
In 1994, I became president of the American Culinary
Federation, the largest organization of professional chefs in
the United States. It was a great time for chefs in America,
and my goal as president was to better understand the diver-
sity within the organization and the need for specialization in
culinary education. I was proud to lead the movement to
establish the African American Chefs Association within our
ranks and the Women Chefs of America. At our annual con-
vention in 1995, a small group of chefs involved in product
development met to discuss the concept of their own asso-
ciation to meet the needs of the research chef community
that was slowly growing.
Among the leaders of this group were Winston Riley (who
headed the group and coined the word “Culinology”), Walter
Zuromski, Jeff Cousminer, and our late colleagues Skip Julius,
Bill “Pops” Hahne, and others who wanted their own organi-
zation. By 1996, the Research Chefs Association (RCA) hosted
its first annual conference in Cleveland, Ohio. Finally, there
was an organization representing that group of men and women
who were filling a much‐needed niche: culinary research and
development for food manufacturing.
At one time, chefs and food scientists existed in two differ-
ent worlds, knowing nothing of each other. Now, both were
playing a role in the emerging American food movement. For
chefs wanting to push their creative boundaries, the science
behind the mystique of food was calling.
RCA focused on the scientific and technical curiosities of
food products and was quickly and successfully generating
tremendous interest on the culinary landscape. By 1999, the
RCA touted a 500‐chef membership, including me. In 2000,
the RCA conference was held in New Orleans, and I was
elected to the board of directors. I remember the excitement
as Dan Sortwell, Skip Julius, Greg Grisanti, and Joe O’Connor
were awarded the very first Certified Research Chef certifi-
cates. It was a great day!
Research chefs, or those desiring to become research and
development chefs, found a home within this emerging asso-
ciation. Culinology®, the marriage of culinary arts and food
science, was born, and the first Culinology® class for chefs
was held at Rutgers University in 2001. Jeff Cousminer and his
education committee were working feverishly to expand
Culinology® curricula, first in continuing education programs
and then in undergraduate degree programs throughout the
country. How could one possibly forget the roar of applause
at our conference in 2003 when Donald Moss earned the
first bachelor’s degree in Culinology® from the University of
Nebraska‐Lincoln?
Soon, four‐year degrees in Culinology® were available
at colleges across the country, including Clemson University,
Rutgers University/Mercer County Community College,
the University of Massachusetts‐Amherst, Louisiana State
University/Nicholls State University, Southwest Minnesota
State, and more. And then Culinology® education went
global, when in 2011 the first international bachelor’s degree
program in Culinology® was launched at Taylor’s University
in Kuala Lumpur, Malaysia.
Imagine how proud I was to become national president of
the RCA in 2005. Our marketing campaigns created a buzz
throughout the industry, and RCA members were taking the
stage at annual conferences from the National Restaurant
Association to the International Hotel/Motel Show. Our
membership growth was constant, and our strategic partner-
ships were strong. Our educational programs and certifica-
tions continued to become the reason for belonging to RCA. I
am so proud to have been in a position to see Culinology®
become a sought‐after industry standard with more and
more national and international food companies seeking
Culinologists in their kitchens, thereby growing the value of
this unique brand. Our national headquarters was set up in
Atlanta, and alliances with the Institute of Food Technologists
were established.
Now, within the ranks of RCA, the book on the subject has
been written! And what better way to mark the 20th anniver-
sary of the Association than with the publication of
Culinology®: The Intersection of Culinary Art and Food Science.
I am ecstatic to see it become a reality under Jeff Cousminer’s
vision. Congratulations to everyone who has made this long
anticipated book a reality, and to Jeff for inspiring others to
achieve culinary research greatness as well.
—Chef John Folse
FOREWORD • v
ffore.indd 5 1/14/2016 8:49:41 AM
vi CULINOLOGY®
Foreword to Culinology®:
The Intersection of Culinary Art and Food Science iv
Preface So What the Heck Is Culinology®,
Anyway? viii
J. Jeffrey Cousminer
Jim (J.D.) Pintner, CRC®
Acknowledgments xiii
Research Chefs Association
Chapter 1: The Business of New Product
Development and the Role of the Culinology®
Professional 1
Mark Crowell, CRC®
Barb Stuckey
Chapter 2: The Principles of Food Science 19
Lisa J. Mauer, Ph.D.
M. Fernanda San Martin-Gonzalez, Ph.D.
Chapter 3: Review of Cooking Techniques 46
Charlie Baggs
Herbert A. Stockschlaeder II, CRC®, DTR
Chapter 4: Protein-Based Foods:
Introduction and Red Meats 57
James W. Lamkey, Ph.D.
Chapter 5: Protein-Based Foods: Poultry 77
L. Cain Cavitt, Ph.D., CCS®
Contributor: Jason M. Behrends, Ph.D., CCS®
Chapter 6: Protein-Based Foods: Seafood 85
Lucina E. Lampila, Ph.D., R.D., C.F.S.
Contributor: Jeff Cowles, CCS®
Contents
Chapter 7: Protein-Based Foods: Vegetable
Sources of Protein and Protein Complementation 106
Natalie Pitchford Levy, CCS®
Contributor: Hinnerk von Bargen, CHE
Chapter 8: Carbohydrate-Based Foods 128
Allen J. Freed
Dan Putnam
Contributors: Hinnerk von Bargen, CHE
Jason R. Gronlund
Chapter 9: Lipid-Based Foods 166
Marilynn Schnepf, Ph.D., R.D.
Lynne Morehart
Brian K. Yager
Chapter 10: Egg- and Milk-Based Foods 180
John U. McGregor, Ph.D.
Julie K. Northcutt, Ph.D.
Michelle Parisi, Ph.D.
Tonya C. Schoenfuss, Ph.D.
Christian Thormose
Chapter 11: Fermentation 196
James R. Adams, CCS®, CFS
Charles Hayes, CRC®, CEC
Chapter 12: Food Additives 220
Andres V. Ardisson Korat, MS, CCS®
Melissa Haupt, CRC®, CEC
Jerome Lombardo
Chapter 13: Food Safety and Spoilage 245
O. Peter Snyder, Jr., Ph.D.
Rachel B. Zemser, MS, CCS®
ftoc.indd 6 1/14/2016 9:32:39 PM
CONTENTS • vii
• Chapter 14: Shelf-Life Extension 286
Klaus Tenbergen, Ed.D., CMB, ASBPB, MCFE
Priscila D. Santiago-Mora
Dennis Ferris, Ph.D.
Contributors: Dominic Man. BSc, MSc, FIFST,
Shirley VanGarde, Ph.D.
• Chapter 15: Food Packaging 305
Dr. Aaron L. Brody
Mark Thomas
Contributors: Thomas Trimarco, Jr.
• Chapter 16: Developing Nutritious
Food Products 321
Darryl L. Holliday, Ph.D., CRC®
Margaret D. Condrasky, Ed.D, R.D., CCE
Marie Hegler
Contributor: John W. Finley
• Chapter 17: Sensory Evaluation 352
Witoon Prinyawiwatkul, Ph.D.
Robert Delaney
M. Michele Foley
Dustin Hilinski
Howard R. Moskowitz, Ph.D.
• Chapter 18: Culinology® Applications
in Food Processing—From the Chef’s
and Food Scientist’s Perspective 365
Mark A. Uebersax, Ph.D.
Muhammad Siddiq, Ph.D.
Carl P. Borchgrevink, Ph.D.
• Chapter 19: Commercializing the Culinary
Gold Standard 396
Marilyn Carlson, CFS
Robert Danhi
Craig “Skip” Julius, CRC®, CCS®, CEC, CCP
Index 414
ftoc.indd 7 1/27/2016 12:02:41 PM
viii • CULINOLOGY®
Preface: So What the Heck Is
Culinology®, Anyway?
J. Jeffrey Cousminer, Past President, Research Chefs Association; R&D Manager, Stonewall Kitchen
Jim (J.D.) Pintner, CRC; Consulting Chef, Sandridge Food Corporation
As chefs, the foods we handle every day have deep scientific
underpinnings. But approaching the whole subject of food
science can be scary to people who equate it with long‐
forgotten and even intimidating high‐school chemistry.
Yet once a chef gains the kitchen skills necessary to work
through the most challenging meal service periods and create
awesome signature dishes, what’s next? For successful chefs
who really want to push their creative boundaries, the science
behind the mystique of food is calling. The next challenge is
exploring the origins, the make-up, and the scientific and
technical curiosities of the foods we devote ourselves to every
day. Enter Culinology®.
Simply put, Culinology® helps us understand how and
why things happen to food during the preparation and cook-
ing process, and once learned, how we can manipulate them
to our advantage. Have you ever picked up a jar of commer-
cial mayonnaise, looked at the ingredient statement on the
label, and wondered why it was different from the mayon-
naise you might make in your kitchen? Well, each ingredient
in the jar is necessary for a commercial product to enter and
survive in the mass marketplace. And this is true for more
than mayonnaise. Today’s supermarket shelves are lined with
products carefully developed to satisfy the savvy tastes of our
food‐obsessed nation while maintaining high standards of
quality, safety, stability, and affordability.
Today, the public wants and expects their favorite foods to be
available everywhere, from the finest restaurants to supermarket
shelves. But how does this happen? How does a chef who has
mastered the most creative restaurant menu items take one of
them and reproduce it to meet the demands of a mass-market
launch? How is restaurant food transformed into a mass‐pro-
duced item while maintaining quality and authenticity but with-
out incurring staggering costs? How do the culinary skills of the
chef and the food science experimentations of the product devel-
oper work together to create delicious food that won’t perish
before it reaches the consumer? The answers are found in the
study of Culinology®.
Culinology® blends the underlying principles of food
science and technology with the chef ’s skilled art of culinary
creativity and originality to satisfy public tastes in the
marketplace.
Culinology®: The Intersection of Culinary Art and Food
Science, by the Research Chefs Association, is designed to
help professional chefs (as well as culinary students and
product developers) approach the science behind the foods
they are developing in their labs and test kitchens, and learn
how to apply Culinology® to the challenging and rewarding
career of new product development (NPD).
The trained Culinology® professional plays an important
role in the development of a food item from concept to mass‐
market launch. Let’s return to the example of mayonnaise.
Chefs find it fairly easy to make mayonnaise in a restau-
rant setting: Blend together egg yolks, vinegar or lemon juice,
sugar, salt, and a touch of mustard, and then slowly drizzle in
a generous measure of vegetable oil while whisking rapidly;
very soon, the mixture starts turning lighter and thicker and
shinier and . . . voilà: mayonnaise! In comparison, look again
at the ingredient statement on the label of a jar of commercial
mayonnaise. Why is there a difference between the two?
The difference is that in the much larger scale of the new
product development world, additional considerations must
be taken into account, such as safety, shelf life, nutritional
value, and ingredient and production costs. A lot is riding on
these decisions. The Culinology® professional understands
the full cost of developing a product from start to finish and
the risks entailed if the product fails.
For example, let’s say you want to develop a better version
of commercial mayonnaise. This book will help you identify
and explore questions such as these before you begin:
• What is different about this better mayonnaise that will
make it competitive (and successful) in the marketplace?
• Will it be a standard mayonnaise (containing eggs, full
fat, etc.), or will it be a specialty product that may not
fpref.indd 8 1/18/2016 10:42:40 AM
meet the government’s standard of identity for mayon-
naise (and will therefore require special labeling)?
• Will any of the ingredients require call-outs on the label
for allergens (eggs or soy), vegan (egg-free), or other label
claims (low fat, reduced sodium, good source of omega‐3
fatty acids, organic, GMO-free)?
• Will it be packaged in glass or plastic? pails? jars?
pou ches? tubes?
• Will it be available in multiple sizes: large bulk packages,
gallons, quarts, pints, single‐serve squeeze packs?
• Will it be marketed to industrial accounts to be used as
an ingredient in another manufacturer’s products, or to
foodservice—that is, the restaurant trade—or to retail
supermarkets or specialty stores? Or all of the above?
• How will you ensure shelf stability and safety?
• Will the new product run on standard machinery, or will
it require capital investment in new equipment?
• How will you tell when you’ve achieved your objectives
(sensory, health or other benefits, preference over mar-
ket leaders, profitability, and so on), and how can you
predict success in the marketplace?
• Once the product is launched, how will you measure its
success, and how can you ensure continuing success?
And that is only the beginning!
Through the study and application of Culinology®, more
food scientists are exploring culinary arts and more chefs are
studying food science with the common goal of giving
consumers the best possible foods while ensuring product
profitability. This book will help you begin thinking like a
Culinology® professional by explaining what it takes to bring
a product to market.
Culinology® is a shift in how we think about food and food
production. No longer must food science and culinary arts
work in isolation; they have formed a symbiotic marriage,
mutually dependent and interactive, mutually strengthening
and reinforcing. By studying the science behind the food, you
can become a Culinology® professional: a leader in the
development of a new wave of exciting, crave‐worthy, nutri-
tious, safe, and profitable food products. Welcome to your
future!
Welcome to Culinology ®!
PREFACE • ix
fpref.indd 9 1/18/2016 10:42:40 AM
x • CULINOLOGY®
James R. Adams, CCS®, CFS
Director, Process Optimization,
Tyson Prepared Foods Operations
Charlie Baggs
President and Executive Chef, Charlie Baggs, Inc.
Jason M. Behrends, Ph.D., CCS®
Project Leader, Tyson Foods, Inc.
Carl P. Borchgrevink, Ph.D.
Associate Professor, The School of Hospitality Business,
The Eli Broad Graduate School of Management,
Michigan State University
Aaron L. Brody, Ph.D.
Adjunct Professor, University of Georgia Department
of Food Science and Technology, and President/CEO,
Packaging/Brody
Marilyn Carlson, CFS
Owner/Principal, Dogwood Solutions
L. Cain Cavitt, Ph.D., CCS®
Project Leader, Research & Development,
Tyson Foods, Inc.
Margaret D. Condrasky, Ed.D, R.D., CCE
Associate Professor, Food, Nutrition, and Packaging
Sciences Department, Clemson University
J. Jeffrey Cousminer
Past President, the Research Chefs Association;
R&D Manager, Stonewall Kitchen
Jeff Cowles, CCS®
Research Chef, Kerry Americas
Mark Crowell, CRC®
Principal Culinologist, CuliNex, LLC
Robert Danhi, CCE, CEC, CHE, CCP
Curator of Culture, Chef Danhi & Co.,
Global Flavor Shakers
Robert Delaney
Manager of Product Innovation, Smithfield
Dennis Ferris, Ph.D.
Professor, Department of Food Science and Nutrition,
California State University, Fresno
John W. Finley
Professor and Department Head of Food Science,
Louisiana State University
M. Michele Foley
Director, Sensory and Consumer Insights, Nestlé
Allen J. Freed
President, AJ Freed, LLC
Jason R. Gronlund
VP Culinary, Smokey Bones Bar & Fire Grill,
Johnson & Wales Alumnus
Melissa Haupt, CRC®, CEC
Executive Research Chef, Applebee’s
Charles Hayes, CRC®, CEC
Director of R&D, National Accounts, JMH Premium
Marie Hegler
Food Safety and Nutrition Agent, Cooperative Extension
Service, Clemson University
Dustin Hilinski
Director of Culinary Development, Red Lobster
Darryl L. Holliday, Ph.D., CRC®
Assistant Professor, Our Lady of Holy Cross College
Craig “Skip” Julius, CRC®, CCS®, CEC, CCP
Manager of Culinary Solutions, Sensient Flavors
Andres V. Ardisson Korat, MS, CCS®
Doctoral Student, Harvard, T. H. Chan
School of Public Health
James W. Lamkey, Ph.D.
Director of Technical Services, Spicetec Flavors
and Seasonings
Lucina E. Lampila, Ph.D., R.D., C.F.S.
Food Safety Institute
Natalie Pitchford Levy, CCS®
Quality Control Manager, Bedemco, Inc.
Jerome Lombardo
Certified Flavor Chemist
Contributors
fcontri.indd 10 1/14/2016 8:47:06 AM
CONTRIBUTORS • xi
Dominic Man, BSc, MSc, FIFST
Principal Lecturer, Department of Applied Science,
Faculty of Engineering, Science, and the Built
Environment, London South Bank University
M. Fernanda San Martin‐Gonzalez, Ph.D.
Associate Professor, Department of Food Science,
Purdue University
Lisa J. Mauer, Ph.D.
Professor, Department of Food Science, Purdue University
John U. McGregor, Ph.D.
Professor, Food, Nutrition, and Packaging Sciences
Department, Clemson University
Lynne Morehart
Technical Service Manager, Cargill Oils and Shortenings
Howard R. Moskowitz, Ph.D.
Chairman, iNovum LLC Chairman, Mind Genomics
Associates
Julie K. Northcutt, Ph.D.
Professor, Food, Nutrition, and Packaging Sciences
Department, Clemson University
Michelle Parisi, Ph.D.
Food, Nutrition, and Packaging Sciences Department,
Clemson University
Jim (J.D.) Pintner
CRC®, Consulting Chef, Sandridge Food Corporation
Witoon Prinyawiwatkul, Ph.D.
School of Nutrition and Food Sciences, Louisiana State
University Agricultural Center
Dan Putnam
Technical Manager, Grain Processing Corporation
Priscila D. Santiago‐Mora
Food Industry Engineer, Instituto Tecnológico y de Estudios
Superiores de Monterrey, Campus Querétaro, México
Marilynn Schnepf, Ph.D., R.D.
Professor, Nutrition and Health Sciences,
University of Nebraska
Tonya C. Schoenfuss, Ph.D.
Associate Professor, Department of Food Science
and Nutrition, University of Minnesota
Muhammad Siddiq, Ph.D.
Associate Professor, Department of Food Science
and Human Nutrition, Michigan State University
O. Peter Snyder, Jr., Ph.D.
President, SnyderHACCP
Herbert A. Stockschlaeder II, CRC®, DTR
Director of Technical Services,
Rosina Food Products, Inc.
Barb Stuckey
Executive Vice President, Mattson
Klaus Tenbergen, Ed.D., CMB, CEPC, ASBPB, MCFE
Dean of Career Technical Education and Economic
Development, Columbia College, Sonora, CA.
Mark Thomas
President, MDT, Ltd.
Christian Thormose
Chef/Food Production Manager, ARAMARK
Thomas Trimarco, Jr.
Operations/Production Manager, Greencore Rhode Island
Mark A. Uebersax, Ph.D.
Professor Emeritus, Department of Food Science
and Human Nutrition, Michigan State University
Shirley VanGarde, Ph.D.
VanGarde Consulting
Hinnerk von Bargen, CHE
Associate Professor in Culinary Arts,
Culinary Institute of America
Brian K. Yager
Senior National Accounts Development Chef,
Cargill Dressings, Sauces, and Oils
Rachel B. Zemser, M.S., CCS®
Owner, A La Carte Connections, LLC
fcontri.indd 11 1/14/2016 8:47:06 AM
xii • CULINOLOGY®
About the A uthor
RESEARCH CHEFS
ASSOCIATION:
Defining the Future of Food ®
Headquartered in Atlanta, Georgia, the Research Chefs
Association (RCA) is a not‐for‐profit organization dedicated
to the education and professional growth of individuals
engaged in food product development. RCA was founded in
1995 (and incorporated in 1996) by a small group of research
chefs hoping to learn from and share with other like‐minded
professionals facing common challenges in the development
of new food products at the commercial level.
Today, the organization boasts 2000+ members including
chefs, food scientists, food technologists, writers, nutrition-
ists, academicians, researchers, consultants, sales and mar-
keting professionals, suppliers, co‐packers, distributors, and
students. Its mission: Empower the food community to
enhance food experiences through Culinology®, a fast‐grow-
ing approach to food product development that requires a
deep understanding of culinary arts as well as food science.
Having pioneered the discipline, RCA is the premier
authority on the practice of Culinology® and owns the regis-
tered trademark. Today, more than a dozen RCA‐approved
undergraduate Culinology® degree programs in the United
States and Malaysia offer well‐rounded, interdisciplinary cur-
ricula that focus on culinary arts and food science but also
incorporate other aspects of food product development,
including business management, nutrition, processing tech-
nology, and government regulations, to name a few.
The Research Chefs Association also offers a robust
certification program that certifies qualified candidates as
Certified Research Chefs (CRC®) or Certified Culinary
Scientists (CCS®), which, together with the Culinology® degree
programs, provides the food industry with today’s most val-
ued product developers in the world. Culinology® graduates
and RCA‐certified professionals are sought by employers
because they guide innovation in this dynamic industry,
offering unique credentials and powerful, value‐added skills
that are truly Defining the Future of Food®.
fabout.indd 12 1/14/2016 8:46:21 AM
ACKNOWLEDGMENTS • xiii
Acknowledgments
Countless individuals have played a role in bringing this
body of work to fruition. None were more pivotal in its
production than Jeffrey Cousminer and the 56 authors and
contributors responsible for its content. No doubt most of
them would say their individual contributions rest on the
shoulders of those that came before them and those that
work beside them. We’d also like to thank Allison Rittman,
CRC®, for pulling together a small band of educators to
create the teaching tools that support this book in the
classroom setting. A few other hard‐working professionals
also played an important role in the completion of this
book. Not chefs, not food scientists, but professionals from
other fields who believed passionately in this project and
did their level best to move it forward. Special thanks to
Jim Fowler, Tim Kline, and Annaliese Doyle Klainbaum as
well as our taskmaster and fearless editor at Wiley, Andrea
Brescia.
Lastly, we are all indebted to Winston Riley, the man who
laid the foundation for the Research Chefs Association,
brought together the early adopters, set forth its goals, created
its infrastructure, and set in motion the groundswell of support
and recognition that brought us to where we are today. Oh, and
by the way, he invented the term Culinology®. Thank you,
Winston!
fack.indd 13 1/14/2016 8:45:41 AM
fack.indd 14 1/14/2016 8:45:41 AM
CHAPTER 1 • The Business of New Product Development and the Role of the Culinology® Professional • 1
1
The Business of
New Product
Development and
the Role of the
Culinology®
Professional
Mark Crowell, CRC®, Principal Culinologist , CuliNex, LLC
Barb Stuckey, Executive Vice President , Mattson
Introduction: Why
Is New Product
Development
Important?
New product development is fraught with difficulty, cost, and
high rates of failure. Why do companies pursue it? Would it
not be simpler to do the minimal amount of development
necessary to stay in business? Or simpler still, wouldn ’ t it be
easier to coast along with existing products?
Both options would certainly be simpler, but the company
would not be profitable for long. Food companies must grow
to make money and survive. As John Maynard Keynes put it
so succinctly in A Treatise on Money, “The engine which drives
Enterprise is not Thrift, but Profit.” 1
Developing new food products is one of the major ways a
food company can build profits. It is estimated that only
one new product idea in 58 actually makes it through the
development process and yields a successful new product. 2
Yet some companies realize a whopping 50 percent of their
sales and 40 percent of their profits from products on the
market five years or less, according to Robert Cooper,
professor of marketing and technology management at
McMaster University in Ontario. 3
Five dominant forces drive the need for new food product
development:
1. Life Cycle
• Nearly all products have life cycles. They enter the
marketplace, flourish for a time, then die and must be
replaced. A very few defy the odds and seem to stay in
the market indefinitely: Kellogg ’ s Corn Flakes®, Spam®,
Kool‐Aid®. But they are the exceptions.
2. Stakeholders ’ Expectation of Growth and Profit
• A company ’ s management may adopt a policy
of aggressive growth to satisfy long‐range business
goals and repay investors or stakeholders. New prod-
ucts are seen as a way to achieve these growth goals.
3. Changing Consumers
• Consumer populations may change due to immigra-
tion, demographic shifts, and a host of other means
of social evolution. These changes may require the
development of new products more suited to the
makeup of the new marketplace.
4. Technology Advancement
• New technology may enable development of new
food products more suited to the lifestyles of current
consumers. It may also offer increased assurances of
food safety, higher food quality, or greater efficiency in
production.
5. Evolution in Regulations or Public Health
• Changes in government legislation, health programs,
agricultural policy, or agricultural support programs
may dictate (or support) the development of new
food products.
Sources of Growth
Most companies exist to generate profit. Senior manage-
ment, under the direction of the owners or the shareholders,
follows a corporate business plan that sets out specific finan-
cial and growth objectives.
a
s
t
e
ne
du
ent
the
s
ct
t
he
o
a
he
c01.indd 1 1/14/2016 7:09:36 AM
2 • CULINOLOGY®
Food companies can achieve growth in a limited number
of ways:
1. Expanding into New Markets
Many companies that launch a product in California
decide they next want to tackle the New York market
because coastal consumers tend to be similar in attitudes,
behavior, and product preference. This can be expensive
for products with short shelf lives due to the need for dis-
tribution in refrigerated or freezer trucks. Extremely per-
ishable fresh products may last only a few days, making
cross‐country distribution impossible under any condi-
tions. A distribution system and its cost may limit expan-
sion. Export markets present their own unique risks.
2. Growing Market Share
One of the most logical places to find customers for a new
product is to grab them from a competitor. This means of
growth is about trying to achieve market penetration and
share within existing markets by slugging it out with com-
petitors. Large sums of money are required for advertising
and promotion, buying shelf space (slotting allowances),
and funding sampling programs.
3. Developing Targeted New Products
New products can contribute to growth and profitability.
For example, as the baby boomer population ages, a food
company may decide to develop new products that offer
benefits that appeal to aging seniors.
At the same time, another company may decide that
aging baby boomers moving into retirement (and, hence,
fixed incomes) may no longer be able to afford their prod-
ucts (or services—for example, dining in expensive restau-
rants). Their new approach may focus on developing
products to target the younger generation of consumers—
for example, the Millennials (those who came of age
in 2000 and are at the beginning of their prime earning
years).
Either of these new consumer targets could provide a
huge profit opportunity. There are, however, associated
costs with each, to be explained later in this chapter.
New products can also help a company differentiate its
offerings, reducing the need to compete on price, which
often occurs when competitors enter an existing market
and drive profit margins down.
4. Cost Containment
Reducing expenses and overhead costs is also a common
practice during a company’s growth phase. Methods of
reducing expenses include reducing staff, implementing an
energy conservation program, improving process efficiency,
adopting a waste management program, streamlining pro-
curement, negotiating better prices, and adopting a process
and quality control program (to reduce losses through over-
fill, waste, and product returns). These thrift measures may
help companies’ profitability but are of limited value to
growth. Companies may own food or beverage manufac-
turing plants that cost hundreds of thousands—if not
millions—of dollars to build, purchase, or lease. These
plants often carry mortgages or rental payments. As a
result, it behooves companies to make sure these fixed
assets operate at full capacity. An underutilized plant is a
drain on resources because it costs money to maintain
whether it is producing a low or high volume of goods. The
more goods it cranks out (maximizing throughput), the
more income the company can make to pay for its opera-
tion and maintenance.
So companies usually decide to develop new products
that can be manufactured in their existing plants. If these
products can be produced during the off (or slow) season,
this makes new product development even more attrac-
tive, as there is incentive to spread production evenly
throughout the year. This keeps trained workers continu-
ously employed, reduces plant overhead, provides a stead-
ier cash flow, and benefits the community (provides jobs,
raises the standard of living, increases discretionary spend-
ing, and improves the tax base). A plant operating year‐
round is more profitable than one that is idle part of the
year, and a plant operating at full capacity is usually more
profitable than one operating at reduced capacity.
The Food Industry
The U.S. food processing industry is often taken for granted.
Except in times of crisis, such as a national food poisoning
outbreak (like the salmonella‐contaminated peanut case in
2009), it has become a feature of the economic landscape so
unremarkable as to be nearly invisible.
The food industry may appear unremarkable for many
reasons. Many processed food products that were wildly
innovative when originally developed many years ago
(canned foods, frozen foods, etc.) are now so familiar in this
high‐tech age that they are viewed as humdrum. Another
reason may be that the food industry runs so incredibly effi-
ciently. In fact, in the latter part of the late twentieth and early
twenty‐first centuries, the food production, distribution, and
retailing systems were optimized to the point where cereal
seemed to sprout from the shelves of Safeway, Kroger, and
Wal‐Mart, meat and poultry came from the deli or fresh
meats counter, and produce grew itself into plastic trays in
the produce section. We became completely separated from
where our food actually came from.
Food processing used to be closely linked to agriculture
or domestic household activities. Many processing indus-
tries were originally part of farm operations (such as butter‐
and cheese‐making) or were based on domestic kitchen
skills (pickling, canning, baking). Modern food processing is
more like that of other mass-manufactured products (such
as cell phones, clothing, and cars) than the local crafts of the
recent past. The chasm between farming and processing is
widening.
Some operations employed by food processors, such as
flour milling, are indeed ancient in their origins, but the
methods and equipment in use today bear little resemblance
to ancient gristmills. Most of today’s food processing tech-
nologies are the result of modern scientific discoveries and
decades, if not centuries, of technological refinement.
c01.indd 2 1/14/2016 7:09:36 AM
Food Development
Today
The food marketplace is a constantly changing organism; it is
never static. Several factors contribute to this dynamism.
Today’s consumers are bombarded with food media. With
round‐the‐clock cable network food programs, splashy food
magazines and cookbooks, morning and evening news pro-
grams showcasing cooking, and the celebrity status of big‐
name chefs, the average U.S. consumer is now savvier about
food than ever. For many people, food preparation has gone
from daily drudgery to a medium for creative expression,
relaxation, and entertainment.
As a reaction to the evolving food culture, consumers
now have more choices than ever. With the ability to buy
food via warehouse stores, mail‐order catalogs, convenience
stores, ethnic groceries, specialty food stores, farmer’s mar-
kets, the telephone, the Internet, and increasingly, directly
from farmers, consumer buying habits are changing. Because
of this, the traditional supermarket has had to adapt. For
example, some grocery stores, such as Whole Foods, have
become a collection of food boutiques surrounding the tra-
ditional center‐store aisles of staples. An abundance of res-
taurants, cafés, fast food outlets, service delis, gourmet
bakeries, smoothie bars, fresh juice stands, and store‐made
sushi and pizza are all available at a wide range of supermar-
kets (or “hypermarkets” as some of the larger stores like to
call themselves). These operations give consumers many
ready‐to‐eat, prepared food options where they used to just
purchase food for cooking or reheating at home.
The consumer has a strong influence on the food and bev-
erage marketplaces. But who is the consumer?
The profile of consumers in any marketplace, particularly
the retail marketplace, is constantly changing. The result is a
change in buying habits. Many factors cause this. Population
movement brings changes in the ethnic background of neigh-
borhoods, and with these shifts come consumers with differ-
ent food needs. Populations, and thus neighborhoods, age.
Formerly vibrant city neighborhoods may decline due to eco-
nomic downturns. Empty downtown city neighborhoods
may enjoy a rebirth as a fashionable area for young profes-
sionals. Most geographic market areas are in a constant state
of flux with respect to the ethnic makeup, incomes, educa-
tion, and lifestyles of their consumers. As both consumers
and marketplaces change, food manufacturers serving those
market sectors must respond to those changes quickly.
This fluidity in the marketplace must be accepted by the
food processor as a challenge (and a never‐ending source of
work for Culinology® professionals!). No single product can
answer all the demands of consumers all of the time. New
products are needed to satisfy emerging market niches, new
lifestyle trends, and changing demographics. The subtle and
not‐so‐subtle changes in the marketplace can be a great
motivator for product development.
Another major factor driving the marketplace is competi-
tion. A competitor can launch a new and improved product
into the marketplace at any time. This can instigate a reaction
from any company whose sales might suffer from the new
product. This retaliatory action may involve new pricing strat-
egies, promotional activities, or the development of new prod-
ucts to combat the competitor’s intrusions.
Product Life Cycles
Every product has a life cycle. When first introduced, new
product volumes are low. It takes time to build a business.
Volume grows as consumers buy, are satisfied, and make
repeat purchases. In time, competitors enter the market,
which eventually becomes saturated; sales volume levels off
and gradually declines as consumers grow bored with or
switch to other products.
A product life cycle has five phases:4
The Stages of a Typical New Product Life Cycle
1. New product development
• very expensive
• no sales revenue
• research and development costs
2. Market introduction
• high cost (retail slotting fees)
• low sales volume
• no known competition; competitive manufacturers
watch for acceptance/segment growth
• continued research and development costs
• demand must be created
• customers must be prompted to try the product
3. Growth
• significant increase in sales volume
• costs reduced due to economies of scale
• growth of profitability
• increased public awareness
• increased competition, with a few new players enter-
ing the market
• margins sacrificed to allow lower pricing to maximize
market share (for trade deals, trial coupons, etc.)
CHAPTER 1 • The Business of New Product Development and the Role of the Culinology® Professional • 3
Growth
Stage
Maturity
Stage
Decline
Stage
Sales
Volume
Units
Costs
Quantity
$
Intro-
duction
Stage
FIGURE 1.1 Product life cycles.
Source: Mark Crowell, CRC®.
c01.indd 3 1/14/2016 7:09:36 AM
4 • CULINOLOGY®
4. Maturity
• lower costs in an established market (less need for
publicity)
• sales volume peaks
• increase in competitive offerings
• prices tend to drop due to the proliferation of com-
peting products
• brand differentiation, feature diversification as each
player seeks to differentiate from competition
• still very profitable
5. Decline or Stability
• costs become suboptimal
• sales volumes plateau or decline
• prices, profitability diminish
• more profit comes from efficiencies in production/
distribution rather than increased sales
Profit is always a better indicator of new product success
than sales.
The introductory phase (2) shows minimal net profit. This
period bears the cost of research and development as well as
the additional heavy costs of promotion and market penetra-
tion. Business (and profit) continues to improve throughout
the growth phase (3) but eventually drops off as costs for
market expansion take their toll. During the maturation
phase (4), costs to support the product match profits as sales
rapidly drop off. The company eventually sees the product as
an unprofitable item that cannot be maintained. Manufacture
of the product ceases (5).
To maintain the viability of the company, replacement prod-
ucts must be launched. The company should not wait for profit
to dip as its product loses ground in the marketplace. It must
keep a series of new products in various stages of development
at all times. A good rule of thumb is that for every one product
on the national market, two should be in test markets, and sev-
eral others should be in the last stages of consumer testing. The
cumulative profitability of these new food products, if they are
successful, assures a good long‐term return on investment.
Some products, of course, defy the odds and become long‐
term successes, finding a permanent home on supermarket
shelves. But they are the exception. These successful prod-
ucts serve another function: As proven winners, they become
the base for new variations. Products launched as variations
of a successful product require less risk and upfront invest-
ment than truly new and unique products.
Technological
Influences on New
Product Development
Scientific discoveries provide insights into the physical world,
human health, the environment, and materials. This knowl-
edge can then be applied to processing technologies that lead
to innovative new products and services.
Using today’s Internet, any company can access vast quan-
tities of business and technical information that previously
were accessible only in specialized libraries in major urban
centers. Expert technical information to assist in product
development programs is available to even the smallest food
processing company in remote regions of the country.
One can find many examples of technical advances in food
packaging. A multitude of materials are now available, where at
one time the packaging industry relied primarily on tin‐coated
steel, glass, and aluminum. The three‐piece can, with its lead‐
soldered side seam, was once the mainstay of ambient long‐
shelf‐life product packaging. This gave way to the two‐piece
seamless can. Nowadays, plastic‐coated paperboard, compos-
ites of aluminum, plastic, and paper, and even edible food car-
tons can preserve and protect the high quality and shelf life of
foods. Containers are also now available in microwavable, dual
ovenable, biodegradable, recyclable, and compostable forms.
Greater knowledge of food spoilage mechanisms and
preservation technologies has given rise to new products
with better safety, stability, quality, taste, and nutrition.
For example, improvements in retort technology and equip-
ment have elevated canning, historically the blue‐collar
workhorse of food preservation, to a contemporary process-
ing method that improves texture, color, flavor, and con-
venience without the need for a can—a relatively heavy and
expensive package that isn’t microwavable and produces a
lot of solid waste. New ingredient technologies can enhance
flavor, texture, and other product attributes while protecting
them during severe processing procedures.
Advances in nutrition science have produced a growing
awareness of the role food plays in our health—specifically,
the relationship of certain foods to disease prevention. The
food industry now has an entirely new class of foods called
nutraceuticals (nutrition + pharmaceutical = nutraceutical,
meaning foods with health benefits beyond basic nutrition)
purporting to help prevent cancer, heart disease, aging, and
other ailments. This topic is discussed in later chapters.
Food products are now recognized as much for the
absence of harmful ingredients (sodium, cholesterol, satu-
rated and trans fats, and refined sugars) as for the presence of
beneficial ones (calcium, dietary fiber, mono‐ or polyunsatu-
rated fats, antioxidants, vitamins).
Food processors and brand owners cannot afford to be
unaware of developments in science and technology and the
impact of these advances on consumers and food retailers,
particularly given the growing role of social media and the
public‐relations savvy of watchdog groups such as the Center
for Science in the Public Interest.
Food Industry
Segments
When consumers make food choices, they have an incredible
number of places where they can satisfy their hunger. Millions
of meals are eaten every day, and they are eaten in almost as
many different locations. The industry is segmented in many
ways. The U.S. Department of Agriculture’s (USDA) Economic
Research Service (ERS) and most food businesses categorize
c01.indd 4 1/14/2016 7:09:37 AM
the industry into two segments: food eaten at home versus
food eaten away from home.
Food‐at‐home refers to foods and beverages purchased at
traditional food retailers like supermarkets. However, the
term may be a bit misleading. Food‐at‐home may in fact be
consumed outside the home in the form of lunchboxes car-
ried to school or work. It also may include prepared foods
purchased at a food‐at‐home retailer and eaten somewhere
other than home. For example, a sandwich purchased at a
grocery store and eaten in the car (“dashboard dining”) might
still fall under the heading of food‐at‐home because it was
purchased at a grocery store, which is classified as a food‐at‐
home retailer by the ERS. Recently the growth in prepared
foods within traditional retailers, such as convenience stores
and grocery stores, is changing how companies classify pre-
pared foods within the traditional food‐at‐home category.
An outgrowth of this changing mindset was the 2008 inaugu-
ral Foodservice at Retail Expo, a conference focused on prepared
foods sold in traditionally food‐at‐home retail environments.
The Foodservice at Retail Expo also explored non‐food retail
environments such as bookstores, airports, and drugstores,
many of which offer food and drink and are now becoming
much more sophisticated in their food and beverage offerings.
Food‐away‐from‐home refers mainly to purchases at restau-
rants but also to “food purchased at such outlets as hotels and
motels, recreational places, vending machines, and schools and
colleges and food provided to domestic military personnel.”5
Using the human stomach as a metaphor for a pie chart, con-
sider that a human being can eat only a finite amount of food.
This entire amount constitutes 100 percent “share of stomach,” a
term used to indicate what percent of the food dollar is spent on
a particular type of food or in a particular channel of food distri-
bution. A highly influential 2005 study by the consulting firm
McKinsey Co. predicted that foodservice would continue to gain
on and eventually overtake sales in retail stores (then almost
evenly divided between the two channels). Contrary to their pre-
dictions, U.S. consumers have steadily increased purchases at
retail while cutting spending at foodservice.6 The global reces-
sion of 2008 and 2009 further hurt food‐away‐from‐home sales
as consumers cut back on dining out. Retail sales data through
the first half of 2013 indicate that retail remains ahead of food-
service 52 percent to 48 percent (see Figure 1.2).
Food-at-Home
The largest food‐at‐home segment is traditional grocery
stores—the stores we think of as supermarkets. U.S. shoppers
made an average of 1.9 trips to the grocery store per week in
2007. That year, a typical grocery store averaged about 47,000
square feet and carried an average of 45,000 items.
There was a flurry of consolidation in the supermarket
industry in the late 1990s and early 2000s. As a result, the larg-
est supermarket companies operate a number of store chains
under many different brand names, a carryover from the days
FIGURE 1.2 Monthly retail sales for food‐at‐home and food‐away‐from home.
Annual 2013 Year‐to‐Date Cumulative
2009 2010 2011 Mar Apr May Mar Apr May
$ billion
Sales
At home 600.4 52.6% 617.5 52.4% 654.4 52.6% 63.1 58.8 58.8 177.9 236.7 295.5 52.0%
Away from
home
541.4 47.4% 561.8 47.6% 588.9 47.4% 58.1 56.1 56.2 160.3 216.4 272.6 48.0%
Total 1,141.8 100.0% 1,179.3 100.0% 1,243.3 100.0% 568.1
Percent change from year earlier
Sales
At home –1.4 2.8 6.0 4.9 1.2 ‐3.9 2.8 2.4 1.1
Away from
home
–1.4 3.8 4.8 2.3 7.2 2.5 9.5 11.2 11.6
Source: USDA.
Notes: Food sales exclude alcoholic beverages as well as home production, donations, and supplied and donated foods.
Annual food sales data in Table 36 are from Table 1; monthly food sales in Table 36 may not add to the annual food
sales in Table 1 due to differences in Census sampling.
http://www.ers.usda.gov/data‐products/food‐expenditures.aspx#26634
As of January 2011, Table 36 uses revised 2007 Census data.
Source: Based on food sales from monthly and annual data in Census Retail Trade, available at:http://www.census.gov/retail/ Historical
data are available to 2003 in archived agricultural outlook files at:http://www.ers.usda.gov/data‐products/food‐expenditures.aspx
Information contact: Annette Clauson
aclauson@ers.usda.gov
CHAPTER 1 • The Business of New Product Development and the Role of the Culinology® Professional • 5
c01.indd 5 1/14/2016 7:09:37 AM
6 • CULINOLOGY®
when each brand (also known as a banner) was a separate
chain of stores.
In 1988 Wal‐Mart opened its first Supercenter, and the
grocery industry changed forever. Ten years later, Wal‐Mart
accounted for 21 percent of every dollar spent on groceries in
the United States, an incredible feat considering how recently
it entered the grocery retailing business.7 In contrast, Safeway
has been in business since 1915 and Kroger since 1883, and
they both account for smaller percentages than Wal‐Mart.
Other food‐at‐home chain retailers include mass merchants
such as Target, club stores such as Costco and B.J.’s, drugstores
such as Walgreen’s and CVS, dollar stores such as Dollar
General, and convenience stores such as 7‐Eleven and Wawa.
Other non‐traditional chain retailers include natural food stores
such as Whole Foods, which carry a mix of merchandise similar
to traditional grocery stores. Their point of differentiation is
that their products are held to strict ingredient and ethical
standards that offer consumers more natural, organic, locally
sourced, and supposedly healthier alternatives. In addition,
there are also upscale supermarket chains such as Wegmans
and Dean & Deluca that offer its customers a large selection of
premium‐quality products (at premium prices!).
Trader Joe’s is an example of a non‐traditional retailer that
almost defies categorization. The typical Trader Joe’s store is
about 2700 square feet, 10 times smaller than a typical grocery
store, and it sells far more store‐brand products (as a percent-
age of all products sold) than its competitors. Trader Joe’s
sells an average of 80 percent store‐brand products. Yet Trader
Joe’s has a loyal fan base that shops at their stores more fre-
quently than consumers in general shop at their primary tra-
ditional grocer. This helped Trader Joe’s rack up an estimated
$8.5 billion in sales in 2011. As of 2013 there are 400 Trader
Joe’s stores in 39 states and the District of Columbia.8
In the past few decades, these non‐traditional food retail-
ers have taken “share of stomach” away from grocery stores.
According to the ERS, consumers purchased food from non‐
traditional outlets 13.8 percent of the time in 1988. In 2006,
this number had increased to almost 33 percent of at‐home
food purchases.
Food-Away-From-Home
Food‐away‐from‐home goes by many different names: food-
service, on‐premise, and, most familiarly, restaurants. In the
1970s, food‐away‐from‐home represented about 18 percent
of U.S. daily caloric intake and about one‐third of the dollars.
In 2004 (the latest USDA numbers available), U.S. consumers
spent half their food dollars on food‐away‐from home (ERS).
The restaurant industry is generally categorized into seg-
ments that refer to service style. At the less expensive end of
the industry are Quick‐Serve Restaurants (QSRs). These are
relatively inexpensive places with a walk‐up counter and/or
drive‐through window, and they do primarily take‐out busi-
ness. In years past they were identified by the term fast food,
which has developed a derogatory connotation.
The largest, most widely known (and geographically wide-
spread) QSRs are mostly large multi‐unit (and multinational)
chains. McDonald’s and Subway are the largest, each with
30,000+ restaurants worldwide. Smaller, independent QSRs like
the 260‐unit chain In‐N‐Out Burger in California, Nevada,
Arizona, and Utah, and the single‐unit operation Box Car
Burgers in Ellensburg, Washington, exist in almost every market,
but there are fewer independent QSRs every year as the cost of
doing business for independents makes it difficult to survive.
QSRs traditionally serve burgers, fries, tacos, and fried
chicken. Today you can get almost any type of food in a QSR
format. For example, Panda Express offers quick‐serve
Chinese food, Fazoli’s offers quick‐serve Italian, and Jamba
Juice offers quick‐serve fruit smoothies and juices.
Fast casual restaurants are similar to fast food restaurants
in that they feature walk‐up counter service but have more
upscale interiors, a higher proportion of diners eat their meals
FIGURE 1.4 Share of stomach.
1988 % Share of Stomach
Traditional Retailers 86.20%
Nontraditional Retailers 13.80%
2006 % Share of Stomach
Traditional Retailers 66.67%
Nontraditional Retailers 33.33%
100.00%
Source: Mark Crowell, CRC®.
FIGURE 1.3 The ten largest U.S. retailers
of food and beverage.
Company Name Top Banners
1 Wal‐Mart Stores Inc.1 Walmart Supercenter,
Walmart Neighborhood
Market, Walmart Express
2 The Kroger Co. Harris Teeter, Ralphs, Kroger
Southwest
3 AB Acquisition LLC Safeway, Albertsons, Vons
4 Publix Super Markets
Inc.
Publix, Publix Sabor, Publix
GreenWise
5 Ahold USA Inc. Stop & Shop, Giant‐Landover,
Giant‐Carlisle
6 H‐E‐B Grocery Co. H‐E‐B, H‐E‐B Plus, H‐E‐B
Central Market
7 Delhaize America Inc. Food Lion, Hannaford
8 Meijer Inc. Meijer
9 Wakefern Food Corp. ShopRite, Price Rite, The
Fresh Grocer
10 Whole Foods Market Whole Foods
1Sales figure represents total annual sales; retailer does not break
out segmented sales by category.
Source: “The Super 50 Food Retailers,” Progressive Grocer, May 2012.
c01.indd 6 1/14/2016 7:09:37 AM
in the restaurant, and average checks are higher. The best-
known fast casual companies are Chipotle Mexican Grill and
Panera Bread. Food offerings at fast casual restaurants are
typically perceived as healthier and fresher, part of the reason
they command higher prices. Chipotle, for instance, promotes
“Food with Integrity,” which includes pasture‐raised pork and
beef and foods free from genetically modified organisms
(GMOs). Panera Bread bakes many of their products fresh
each day in their stores and promotes a food policy of “Clean
Ingredients.” They have committed to removing all artificial
colors, preservatives, sweeteners, and flavors from their menu
by the end of 2016.
At a price premium to QSRs is the next segment of the
restaurant industry: Family Dining. This segment usually
offers table service, otherwise known as full service. The acro-
nym FSR is often used to denote full‐service restaurants,
which are differentiated from QSRs. Examples include
Denny’s, Coco’s, and Sizzler.
Casual Dining is the next segment in terms of increasing
average guest check. Casual Dining offers full table service
and usually serves alcoholic beverages. Examples include
Chili’s, Olive Garden, and Red Lobster.
These days it is highly likely that you will find chefs, food
technologists, and/or Culinology® professionals on the menu
development teams or research and development (R&D)
staffs of all three types of chain dining operations. Culinology®
professionals fulfill many roles at QSR, Family, and Casual
Dining restaurant chains but are most typically responsible
for menu planning and product development. Identifying
menu concepts is something that normally revolves around
consumer research.
The most important consumer research a chain restaurant
Culinology® professional can do is to understand their com-
pany’s core customer. Many times (especially in the QSR
segment) this is referred to as the “heavy user.” Heavy users are
restaurant patrons who frequent the chain more often than
the general population. Once a chain identifies its core cus-
tomers, the job of creating new menu items that appeal to
them becomes much easier.
Trend‐tracking is another responsibility of chain restau-
rant Culinology® professionals. Because most consumers eat
more experimentally in restaurants than at home, the job of
FIGURE 1.5 Supermarket sales by format.
Number of Stores Percent of Total Sales ($ Millions) Percent of Total
Total Supermarkets ($2 million or more) 37,716 100.0% $638,338 100.0%
Supermarket‐Conventional 26,487 70.2 414,794 65.0
Supercenter (Grocery and Mass Merch.)* 4,150 11.0 159,824 25.0
Supermarket Limited Assortment 3,242 8.6 16,106 2.5
Supermarket Natural/Gourmet Foods 3,144 8.3 38,372 6.0
Warehouse Grocery 523 1.4 4,367 0.7
Military Commissary 170 0.5 4,876 0.8
Other Food Retail Formats
Conventional Convenience** 152,120 n/a $412,703 n/a
Gas Station/Kiosk 22,303 n/a n/a n/a
Superette 13,070 n/a 19,974 n/a
Conventional Club 1,320 n/a 136,339 n/a
Military Convenience Store 674 n/a 4,067 n/a
*Supermarket‐type items only
**Excluding gas
Source: Progressive Grocer Magazine.
FIGURE 1.6 Largest restaurant chains ranked
by 2013 U.S. systemwide sales.
1 McDonald’s $35,856.30
2 Subway $12,221.00
3 Starbucks Coffee $11,864.00
4 Burger King $8,501.00
5 Wendy’s $8,354.00
6 Taco Bell $7,800.00
7 Dunkin’ Donuts $6,742.50
8 Pizza Hut $5,700.00
9 Chick‐fil‐A $4,988.50
10 Applebee’s Neighborhood Grill & Bar $4,517.00
Sales and revenue figures are in U.S. millions.
Source: Nation’s Restaurant News. Copyrighted 2015. Penton Media.
11786:0615BN.
CHAPTER 1 • The Business of New Product Development and the Role of the Culinology® Professional • 7
c01.indd 7 1/14/2016 7:09:37 AM
8 • CULINOLOGY®
the Culinology® professional is to identify exciting foods and
beverages that will appeal to the core customer as well as
attract the non‐core visitor (this is known as building traffic).
The last and, some would say top restaurant segment is
called white tablecloth. This term comes from the days when
upscale restaurants always covered their tabletops with
linen. Another term that denotes this segment is Fine Dining.
Most fine dining restaurants are independently owned
and operated. However, multiple‐unit operators like Ruth’s
Chris Steak House and Morton’s have existed for years, and
recent successful chef‐owned restaurant groups like those of
Jean‐Georges Vongerichten, Pano Karatassos’s Buckhead Life
Group, and Emeril’s New Orleans are also finding success
beyond their home markets.
Formally trained food technologists are rare in the fine
dining segment, where culinary arts rule and science is rele-
gated to cooking techniques. The exceptions to this rule are
singular restaurants focused on molecular gastronomy, such
as the Fat Duck in Bray, England; Alinea and Moto in Chicago;
Minibar in D.C.; and Noma in Copenhagen, which tap into
food technology to create new menu items.
The Supply Side
of the Business
Culinology® professionals must work with suppliers whether
they are developing products for the food‐at‐home or
food‐away‐from‐home segments. Suppliers offer opportunity
for employment for Culinology® professionals as well.
Suppliers can range from small, local farmers who raise a
few dozen fowl for fine dining restaurants, to divisions of the
world’s largest food companies, such as Kraft, Unilever,
Nestle, and ConAgra, which supply numerous raw materials
and finished products to the largest chain restaurant and
supermarket operations. All of the larger suppliers employ
culinary professionals to help develop new value‐added prod-
ucts using their ingredients and to help develop strong rela-
tionships with their culinary (and Culinology®) colleagues at
the chain client’s headquarters.
Food Ingredient Suppliers
The food industry segment that once was called industrial
supplier today goes more frequently by the name ingredient
supplier. Rather than providing complete finished products
(entrées, side dishes, sauces, etc.), they tend to specialize in spe-
cific ingredients such as starches, gums, flavors, colors, preserv-
atives, and the like. They often sell their ingredients to the large
food manufacturers mentioned above rather than directly to
restaurants or supermarket chains (the “end‐users”). As such,
they are typically referred to as secondary suppliers, while the
large companies that use these ingredients to manufacture fin-
ished products for the end‐users are called primary suppliers.
A burgeoning part of today’s ingredient sector is compa-
nies marketing ingredients that can be added to food to
increase its nutritional content. Examples of these so‐called
“functional ingredients” include omega‐3 fatty acids (which,
according to the U.S. Food and Drug Administration [FDA],
may reduce the risk of heart disease), probiotic cultures (which
may aid digestion and nutrient absorption in the small intes-
tine), and dietary fiber (which may inhibit colon cancer).
Before they can be used in foods, all food ingredients must
first be classified by the FDA as GRAS (generally recognized
as safe). A GRAS ingredient is “generally recognized, among
qualified experts, as having been adequately shown to be safe
under the conditions of its intended use.”9 There are strict
rules about how the FDA arrives at that conclusion, and it is
the responsibility of food companies to prove that new ingre-
dients meet those requirements. In addition, there are strict
labeling laws that regulate the claims that can be made for
foods using these ingredients.
All Other
It is impossible to list all of the other parts of the food indus-
try that a Culinology® professional might come into contact
with. However, packaging suppliers are critical for ensuring
that food remains fresh, stable, and safe. Packaging is also
critical for displaying a food in an appetizing way. For exam-
ple, consider how the appearance of packaging affects your
opinion of frozen entrées in the freezer case.
Food equipment manufacturers, agricultural co‐operatives,
and agri‐business companies are just a few of the other types
of companies that can benefit from the services of Culinology®
professionals.
Governmental
Influences on New
Product Development
The objectives of government with regard to food legislation are:
1. To ensure that the food supply is safe and free from con-
tamination within the limits of available knowledge and at
a cost affordable by the consumer.
2. To develop, with food manufacturers, standards of compo-
sition for foods as well as labeling standards.
FIGURE 1.7 Largest family dining restaurant
chains ranked by 2013 U.S. systemwide sales.
1 International House of Pancakes $2,650.00
2 Denny’s $2,379.00
3 Cracker Barrel Old Country Store $2,012.00
4 Waffle House $1,007.00
5 Bob Evans Restaurants $980.00
6 Perkins Restaurant & Bakery $657.70
7 Friendly’s Ice Cream $451.20
8 Big Boy/Frisch’s Big Boy $395.00
Sales and revenue figures are in U.S. millions.
Source: Nation’s Restaurant News. Copyrighted 2015. Penton Media.
117896:0615BN
c01.indd 8 1/14/2016 7:09:37 AM
3. To conduct research, and influence and educate consum-
ers regarding diet and nutrition.
4. To maintain fair trade and competition both domestically
and internationally among retailers and manufacturers in
such a way as to benefit consumers and businesses.
5. To maintain U.S. competitiveness in, and access to, inter-
national export markets.
The first three objectives certainly affect the technical
development of food products and influence marketing and
product development personnel as they create new products
and marketing campaigns.
Government legislation operates at the federal level, the
state or provincial level, and the municipal, local, or county
level. Government at all levels strongly influences the busi-
ness activities of food companies—but in truth, it often lacks
the political will or the muscle to enforce many of its most
critical protections. Examples include major safety inspec-
tion lapses in the case of salmonella‐contaminated peanuts
at Peanut Corporation of America,10 the inability to pinpoint
the cause of a major outbreak of Salmonella Saintpaul in
2007, originally linked to tomatoes,11 and the failure to enforce
inspections in the case of an outbreak of E. coli in spinach in
2008.12 The Food Safety and Modernization Act of 2010 gave
major new powers to the FDA to regulate industry by shifting
its focus from responding to contamination to preventing it.
It is considered the first major piece of federal legislation
addressing food safety since 1938.
The Food and Drug
Administration and
the U.S. Department
of Agriculture
FDA
The Food and Drug Administration (FDA) is an agency of the
U.S. government, within the Department of Health and
Human Services (DHHS). Its charter is to “safeguard the
nation’s food supply by making sure all ingredients used in
foods are safe and that food is free of contaminants.”13
The FDA approves new food additives, monitors dietary
supplements, infant formula, and medical foods, and pub-
lishes them in the Code of Federal Regulations (CFR) annu-
ally under Title 21, Chapter 1, Section 100, which is the
portion that covers food and is the food safety reference for
foodservice operators. The FDA is also responsible for food
labeling (not including meats and poultry, which are the
responsibility of the USDA) and conducts site inspections of
food processing facilities.
USDA
Many areas within the USDA are of interest to Culinology®
professionals. The two most important are the areas of
(1) nutrition and health and (2) food safety.
From the USDA’s website under Food, Nutrition, and
Consumer Services:
[T]he USDA works to harness the Nation’s agricultural abun-
dance to end hunger and improve health in the United States.
Its agencies administer federal domestic nutrition assistance
programs and the Center for Nutrition Policy and Promotion,
which links scientific research to the nutrition needs of con-
sumers through science‐based dietary guidance, nutrition
policy coordination, and nutrition education. Food Safety
ensures that the Nation’s commercial supply of meat, poultry,
and egg products is safe, wholesome, and properly labeled
and packaged.14
Note that the FDA governs most of the food supply, with
these few exceptions: meat, poultry, and eggs, which are
generally within the purview of the USDA within the Food
Safety and Inspection Service. The USDA does not, however,
govern seafood or (non‐egg) dairy products—which fall
under the FDA.
Compounding the influence of these official levels of gov-
ernment are international bodies and quasi‐governmental
agencies like the World Trade Organization (WTO), Food
and Agriculture Organization/World Health Organization
(FAO/WHO), and the International Organization for Stand-
ardization (ISO). These organizations can also impose regu-
lations on manufactured food for international trade and,
indirectly, on new food product development.
• The World Trade Organization (WTO) provides a forum
for negotiating agreements aimed at reducing obstacles
to international trade and ensuring a level playing
field for all, thus contributing to economic growth and
development. The WTO also provides a legal and insti-
tutional framework for the implementation and moni-
toring of these agreements as well as for settling disputes
arising from their interpretation and application. As
an example, the WTO acted on a number of disputes
between countries regarding bovine spongiform enceph-
alopathy (BSE, also known as mad cow disease), most
recently in September 2009 between South Korea and
Canada.
• The European Union (EU) is a unique economic and
political partnership of 28 European countries. EU legis-
lation is made up of Directives and Regulations that
must be implemented by each member state. Directives
define the result that must be achieved but leave to each
member state the choice of form and methods to trans-
pose the directive into national laws. Regulations are
binding in their entirety and automatically enter into
force on a set date in all member states. The EU regu-
lates food safety within member countries through
Regulation (EC) No 178/2002. The EU follows a dual
approach in harmonizing food laws: horizontal legisla-
tion, which covers aspects common to all foodstuffs
(such as additives, labeling, hygiene, etc.), and vertical
legislation on specific products (cocoa and chocolate
products, sugars, honey, fruit juices, fruit jams, etc.). Still
under discussion are legislative initiatives for issues
CHAPTER 1 • The Business of New Product Development and the Role of the Culinology® Professional • 9
c01.indd 9 1/14/2016 7:09:37 AM
10 • CULINOLOGY®
such as maximum acceptable levels for vitamins and
minerals and certain pesticide residues, among others.
• The International Organization for Standardization
(ISO) and the ISO 22000 Standard specifies requirements
for a food safety management system whereby an organi-
zation in the food chain must demonstrate its ability to
control food safety hazards in order to ensure that food is
safe at the time of human consumption. The standard
incorporates the principles of HAACP (Hazard Analysis,
Critical Control Points). ISO accreditation is voluntary,
but many larger companies like the Kraft Heinz Company
and Costco either give preference to suppliers that have
achieved ISO accreditation or require it of their suppliers
before they will do business with them.
• The Codex Alimentarius Commission, under the joint
direction of the FAO/WHO Food Standards Program,
generates internationally recognized standards, codes
of practice, guidelines, and other recommendations
relating to foods, food production, and food safety. The
Codex is broad in scope and covers everything from set-
ting limits on pesticide residues in food to determining
how countries operate their import and export inspec-
tion services. The standards are voluntary and there is
no enforcement mechanism, nor is there an obligation
for member countries to adopt Codex standards; how-
ever, the WTO treats the Codex as an international refer-
ence standard for the resolution of disputes concerning
food safety and consumer protection.
• Regional trade agreements such as the North American
Free Trade Agreement (NAFTA) and many others can
indirectly affect national food regulation, causing unin-
tended consequences. One example involves the U.S.
ban on transportation of food (and other products) from
Mexico into the United States using unapproved vehi-
cles. Rather than resulting in reduced prices on these
products (“Free Trade”), the ban resulted in $2.4 billion
in retaliatory tariffs on U.S. goods, including food prod-
ucts, imposed by the Mexican government when
Congress canceled a Bush‐era pilot project that gave a
select group of Mexican carriers access to U.S. highways
beyond the border commercial zone.
• The Food and Agriculture Organization (FAO) of the
United Nations leads international efforts to defeat
worldwide hunger. It helps developing countries and
countries in transition to modernize and improve agri-
culture, forestry, and fisheries practices and to ensure
good nutrition for all.
• Quasi‐governmental bodies do not have the same leg-
islative powers as the various tiers of government, but
they do have the support of government or the effect
of law, as when the USDA sets the price processors
pay for raw milk when it issues “milk marketing
orders.” These bodies can establish regulations that
participating parties must adhere to. Other examples
are the various marketing boards that exist in many
countries to regulate the local supply, importation,
and price of many food commodities and ingredients
derived from them.
• Influence can also come from professional and trade
associations. These may establish rules of conduct and
wage scales for their members as well as influence gov-
ernment rule‐making through lobbying efforts—as, for
example, when the Organic Trade Association lobbies
the National Organic Standards Board (NOSB), which
makes policy recommendations on the USDA’s National
Organic Program (NOP).
Societal Influences
on New Product
Development
Organic and natural foods have seen tremendous growth. This
growth was spawned by a desire for products grown free of
chemicals, focusing on local agriculture and sustainable crop
and animal husbandry. Many underlying societal forces have
supported this trend, including the aging of the baby boomer
generation, an increasing scientific understanding of the link
between health and diet, and a growing foreboding about the
consequences of dwindling natural resources and humanity’s
impact on the earth’s delicate environmental balance.
Science and technology have also played a large role in
spurring this growth. The development of transgenic genetic
modification, scientific advances in nutrition research, and
studies on global warming and sustainable fishing practices
are just a few of the ways science serves to educate and moti-
vate (either positively or negatively) the public to change their
eating habits. For some consumers, shopping is as much a
political act as a commercial one.
The media play a large role in both informing and shaping
the public’s perception of current food issues. The International
Food Information Council tracks media coverage of food
issues, reporting that in 2000 there were 4000 news articles on
obesity.15 That number climbed to 14,000 in 2006 and rose
to 16,000 during 2007 as various cities and states debated
laws on restaurant nutrition labeling, high‐profile lawsuits
were brought against several quick-service restaurant chains
by obese patrons, and movies such as Fast Food Nation
debuted.
Classification and
Characterization of
New Food Products
What defines a new product? No single definition works
across all facets of the industry. This may explain some of the
disparity in the estimates of introductions and failures.
Simply repackaging an old product may justify its classifica-
tion as a new product.
Likewise, an old, established product may be marketed
in a new segment of the industry—for example, introducing
c01.indd 10 1/14/2016 7:09:37 AM
an existing domestic product to the export market. A new
package size of an old product may also be considered a new
product, and a so‐called “improved” product may be simply
marketing hype or a new package, or may be an actual refor-
mulation of an existing product.
The never‐before‐seen product is the only truly new
product. It is far less common than the other types simply
because it is the most difficult, risky, and costly to develop
and launch.
Each of these new product classifications presents differ-
ent challenges to the food company, whether seen through
the eyes of marketing, manufacturing, finance, or R&D.
Whether new products are introduced into the foodser-
vice, retail, or ingredient segments, they fall into one of the
following classifications:
• Line extensions
• Repositioning of existing products
• New forms of existing products
• Reformulation of existing products
• New packaging or brand labeling of existing products
• New‐to‐the‐company product lines
• New‐to‐the‐world creative products
Not all of these product classifications are truly the result
of product development efforts; some are simply marketing or
procurement exercises. However, all can be important in
extending the product life cycle by generating news for the
product.
Line Extensions
A line extension can best be described as a variation on an
established product—a new flavor of ice cream or a new sea-
soning on a potato chip. Line extensions represent a logical
extension of a family of similar products. They are food prod-
ucts that generally require:
• Less time or effort for development than wholly new
products
• No major manufacturing changes in processing lines or
major equipment purchases
• Relatively little change in marketing strategy
• No new purchasing skills (commodity trading) or raw
material sources
• No new storage or handling techniques for either the
raw ingredients or the final product, meaning that regu-
lar distribution systems can be used
There are gray areas in classifying each type of new prod-
uct. For instance, a ready‐to‐eat (RTE) soup and a condensed
soup are very different products in their marketing and in
marketplace competition, but there are minimal differences
in their manufacture. Even so, they are best not considered as
line extensions of each other. Rather, the condensed soup
and the RTE soup should be classified as very different prod-
ucts marketed to entirely different customers in both the
retail and foodservice channels.
Development of a line extension is normally (but not
always) expected to involve less development time and,
consequently, less development money. The type of product
dictates the amount of effort required for line extensions.
For example, a new flavor of bottled water simply requires
the addition of the new flavor (at an appropriate level) to
the existing base (water). The packaging remains the same,
but a new label will have to be created. On the other hand,
something more complex, such as a new addition to a line
of retorted entrées, will require extensive testing, new ingre-
dient sourcing, modifications to the label (ingredient list-
ings and nutritional facts panels will change), and
submission to the USDA for label approval. The new prod-
ucts also must undergo shelf life testing, including micro-
bial analysis.
Manufacturing is generally not disturbed by such a line
extension, except for the impact on production scheduling
(including additional wash‐down of the line and training for
the line workers) and procurement. Nor are production sys-
tems such as quality control, plant maintenance, sanitation,
and hygiene unduly affected. Minor impact on storage and
warehousing may be felt due to different case sizes and pal-
let configurations, but only if the line extension requires a
different‐size package.
On the other hand, unexpected difficulties may arise. For
example, extending a line of canned beans to make them
more convenient by putting them in flexible pouch packag-
ing may present developmental problems plus manufactur-
ing and manufacturing‐support systems changes. Retort
times, processing and packaging requirements, filling equip-
ment, shelf life, and warehouse storage of the finished prod-
uct may change. Similarly, changes in ingredients may
necessitate extensive changes in processing parameters
because of how the new ingredients react to various steps in
the manufacturing process.
By contrast, if a snack manufacturer extends its product
offerings from potato chips to corn chips to corn puffs to
CHAPTER 1 • The Business of New Product Development and the Role of the Culinology® Professional • 11
Newness to Market
HighLow
N
e
w
n
e
s
s
t
o
C
o
m
p
a
n
y
H
ig
h
L
o
w
New Product
Lines
Completely
New
Line
Extensions
RepositioningsChanges to
Augmented
Product
Core
Product
Revisions
FIGURE 1.8 Newness to market.
Source: Mark Crowell, CRC®.
c01.indd 11 1/14/2016 7:09:37 AM
12 • CULINOLOGY®
peanuts to popcorn, these are not simple line extensions.
Such products have in common only the snack food posi-
tioning. They may be distributed through the same channels
and displayed in the same section of a retail store, but they
should not be considered line extensions. Purchasing strate-
gies, storage facilities, and manufacturing technologies—to
name just three areas of concern—are completely different
for each of these snack forms.
Marketing programs are usually not affected by line exten-
sions, but surprises do occur. If adult flavors are introduced
into a family of snack products originally positioned for chil-
dren, these flavors may not be successful for either adults or
children. Therefore, different promotions, advertisements,
and store placements for the adult products must be consid-
ered. Similar problems might be caused by animal shapes of
pasta in a sauce or children’s bite‐size pieces for cookies and
crackers if the products are to be extended to the adult
market.
Repositioned Existing
Products
A company can be startled to discover, either through con-
sumers’ letters or product usage surveys, that their consum-
ers have come up with a new use for an existing product,
giving it unexpected new life. The use of baking soda as a
refrigerator deodorizer is an example of such repositioning.
The promotion of soft drinks from leisure‐time beverages to
meal accompaniments, especially in the breakfast market, is
another repositioning that has proved worthwhile, as
younger consumers do not take to coffee the way prior gen-
erations did. According to The NPD Group (formerly known
as the National Purchase Diary), a market research company
that monitors consumer purchase data from over 165,000
stores, consumers eating breakfast outside the home order
soda pop with 15.1 percent of their breakfasts, compared
with 7.9 percent in 1990. Of meals eaten at home, 2.4 percent
now include carbonated soft drinks, compared with only
0.5 percent in 1985.16
Oatmeal became perceived as a health food on the basis
of the FDA’s approval of fiber claims as a dietary factor in
reducing cholesterol. Oatmeal and products containing
oatmeal or oatmeal fiber were repositioned as healthy
food, not just warm breakfast options. Cheerios’ soluble
fiber heart health claim has been FDA‐approved since
1997. As another example, in older women with minimal
calcium intake, osteoporosis was identified as a major
health problem. A popular antacid that contains a high
amount of calcium was repositioned as a good source of
this mineral.
The product development time required for repositioning
existing products is minimal. Often, all that is necessary is for
the marketing department to design and print new labels, to
design a new package, or to prepare a new advertising strat-
egy with promotional material. Manufacturing is unaffected.
The responsibility is with the marketing department to capi-
talize on the new niche.
New Forms of Existing
Products
Creating a new product form represents a radical departure
from the type of new product development classifications
previously discussed. It is also the downfall of many new
product ventures. An instantized, solubilized, granulated,
tableted, powdered, solidified, foamed, concentrated,
spreadable, frozen, or otherwise modified version of an exist-
ing food product can involve extensive development time. It
may also require major equipment purchases, both for man-
ufacturing and for packaging. In addition, processing and
the support systems for processing may diverge considera-
bly from the original product. The company may face vastly
different warehousing and distribution system problems.
For this type of product to succeed, it is critical that the
consumer recognizes and appreciates the claimed improve-
ment in the modified product. There must be a perceived
advantage of the new form over the old if the new product
form is to take hold in the marketplace. For example, the
advantage of a dried, sprinkle‐on version of a condiment, such
as mustard or ketchup, may not be appreciated or preferred
over the traditional liquid form because it does not provide
the moisture that consumers associate with and enjoy from
the condiment. However, whipped cream cheese has been
successful. This is because it offers consumers the clear ben-
efit of being easier to spread on breads without needing time
to soften at room temperature. For the company, it offers the
added benefit of a much higher sales price per pound.
The phenomenal successes of instant coffee, foam‐dis-
pensed whipped dairy toppings, and spreadable margarines
continue to tempt many food manufacturers into this class
of development. Miniaturization, that is, bite‐size pieces, has
proven very popular for snack items such as cookies and
crackers, as are 100‐calorie packs of popular snacks. The
new subcategory called calorie‐controlled snacks is currently
worth more than $200 million per year.17 Ninety‐two dif-
ferent 100‐calorie products were on the market as of July
2007, according to Tom Vierhile, director of Datamonitor’s
Productscan Online Datamonitor. That’s up from 51 in 2006,
33 in 2005, and only 13 in 2004. Today, thousands of 100‐
calorie products are on the market.
Reformulation of Existing
Products
The “new, improved” product is typical of this category; the
product is altered in some way but retains its essential char-
acteristics. Reformulation of a product to make some
improvement (for example, better color, better flavor, more
fiber, less fat, greater stability) has a high probability of tech-
nical success. Usually, but not always, reformulation for
improvement can be accomplished comparatively inexpen-
sively and in a relatively short development time.
Reformulation may be necessary for any number of reasons.
For example, an ingredient may have become unavailable, or
new sources of an ingredient must be located. Reformulation
c01.indd 12 1/14/2016 7:09:37 AM
may be needed to lower costs to meet the challenge of cheaper
competitors. It can also allow a company to take advantage of
ingredients with improved characteristics and properties, such
as the line of resistant starches introduced by National Starch
in 2007 that allowed the formulation of products with fewer
calories and a lower glycemic index.
Reformulation may also be necessary to satisfy consumer
demand for healthier products, such as one with fewer calo-
ries. This can also create a new market niche for existing prod-
ucts. High‐fiber bread, low‐fat ice cream, lactose‐free milk
products, and baked goods free of partially hydrogenated veg-
etable oils (a primary source of trans fat) are good examples of
such products. In fact, starting in 2006, the FDA mandated
that trans fats be labeled on all food nutrition facts panels.
This was based on the scientific discovery that trans fats con-
tribute to the risk of heart disease and prompted the entire
food industry to reformulate products to remove or replace all
but trace amount of trans fats. Similar events over the past
decade have centered on removal of monosodium glutamate
(MSG), replacement of GMO (genetically modified organism) ‐
containing ingredients, and the phenomenal recent growth in
the demand for gluten‐free, dairy‐free, organic, and all‐natural
foods. And don’t overlook the size of the Kosher and Halal
markets as possible targets for reformulated foods.
New Packaging or Brand
Labeling of Existing Products
In its simplest form, the packaging of bulk product into smaller
(sometimes individual) packages typifies this category. The
reverse is also true, as many manufacturers make special bulk
packages of their most popular branded products for club store
and foodservice channels. New technologies such as modified
atmosphere packaging (MAP) and controlled atmosphere
packaging (CAP) have permitted the creation of a number of
new products such as prepackaged sliced luncheon meats and
bagged cut‐up salad greens, providing existing products with
an extended shelf life to create new market niches in existing
markets and allow access to new geographical markets.
The packaging and brand labeling of commodity items
such as produce and meats are other examples of existing
products being reborn as a new product. As a classic exam-
ple, chickens were simply chickens until a man named Frank
Purdue (with a remarkably bird‐like face) became famous
through a series of 1970s television ads, asserting that “It
takes a tough man to make a tender chicken.” Angus beef is
another good example.
Here, of course, product development is minimal. Getting
this type of product to market requires purchasing, inspec-
tion, grading, cleaning, trimming, storage, weighing, packag-
ing, and distribution—that is, the same requirements for
bringing an unbranded commodity to market. However, the
responsibility for making the product a success rests mainly
in convincing consumers that the branded item is somehow
better or more trustworthy than the commodity (less expen-
sive) item, and hence more appealing and worthy of com-
manding a higher price.
On the other hand, using new packaging for an existing
product may require the purchase of expensive packaging
equipment. Major changes, such as from metal to glass
containers, require a redesign of the entire packaging line.
Even the change from steel cans to aluminum cans to save
weight, and hence shipping costs, requires an extensive
overhaul of the packaging operation. Similarly, the use of
plastic squeeze bottles with snap‐cap lids for dispensing
mustard, ketchup, or other sauces is a major packaging
change from glass and screw‐top containers. However,
these are one‐time capital investments that should be
recouped over time, assuming that the material and ship-
ping cost savings are realized and the consumer continues
to purchase the product in its new package. Ketchup, mus-
tard, salad dressings, jams, jellies, and syrups have all
undergone this process successfully.
The use of re‐sealable pouches or trays (both thin‐profile
containers) for thinly sliced meats and non‐thermally
processed foods (using high‐pressure pasteurization [HPP])
provides value‐added features such as resealability, improved
convenience, and superior shelf life. However, switching to
such a new style of packaging may require significant equip-
ment investments and product reformulation, such as using
different hydrocolloid systems to maintain meat texture dur-
ing high-pressure processing.
New-to-the-Company
Product Lines
New and innovative products are difficult to categorize.
Generally, the more innovation (change) in a product, the
longer the development time and the higher the research and
development costs will be. Marketing the uniqueness of a
product may be costly because the consumer may need to be
educated as to its benefits. In short, the development of inno-
vative products can be both costlier and riskier than any
other path of new product development.
On the other hand, relatively little research and develop-
ment in terms of costs and time were required for Birdseye to
put individually quick frozen vegetables together with sauce
pellets into a bag 25 years ago. Likewise, putting a can of
tomato sauce, a package of spaghetti sauce spices, and a
package of dry pasta together to make a dinner kit requires
little research and development effort. Over time, both
s cenarios have been extensively copied by many food
companies.
New ingredients can also form the basis for innovative
products. Simulated crab legs, lobster chunks, shrimp, and
scallops based on surimi technology have led to the develop-
ment of many new seafood dishes.
New-to-the-World Creative
Products
Creative, never‐before‐seen products are harder to define
and still more difficult to execute. The relatively recent
transformation of surimi, a fish gel invented several hundred
CHAPTER 1 • The Business of New Product Development and the Role of the Culinology® Professional • 13
c01.indd 13 1/14/2016 7:09:37 AM
14 • CULINOLOGY®
years ago in Japan, into texturized shellfish analogs was
considered a creative product when it first came out. So
were tofu (bean curd) and puffed cereals in their day. Today,
one might consider reformed meat products, steamer tech-
nology packaging, and zero‐calorie bottled salad dressings
as creative developments.
Creative development products are characterized by the
following:
• Extensive development time
• High research and development costs
• Costly marketing to educate consumers about some-
thing new
• High capital costs for equipment, as machinery fre-
quently must be built from scratch if novel processing
steps are involved
• Risky introduction, because consumer reaction is diffi-
cult to predict
• If successful, a flood of imitators who will take advan-
tage of the time and effort developers of the original
product took to create a market for it
In general, the more creative a product is, the greater the
development costs, and development time may be many
months or even years.
Sales and Marketing
Influences on New
Product Development
Brand Management
In the retail food world, companies are organized into divi-
sions by brand or category. There is usually a Brand Manager
who holds responsibility for everything that has to do with
the brand, including managing the brand’s product portfolio,
profit and loss, establishing and maintaining brand equity
(the economic value of the brand name itself ), marketing,
promotion, and spearheading the product strategy. Product
strategy may include working on any or all of the types of new
product classifications discussed previously in this chapter.
Brand Managers are, in essence, “caretakers of the brand.”
They establish new product development parameters; for
example, a brand manager for Ben & Jerry’s might push culi-
nary boundaries with innovative flavor combinations and
unusual ingredients, and encourage marketing the products
with tongue‐in‐cheek names like Chubby Hubby or Chunky
Monkey. At the same time, consumer knowledge of what the
Ben & Jerry’s brand “stands for” communicates that the milk
used to make the product is free of recombinant bovine
growth hormone (rBGH), which would also affect product
development (and cost) if the availability of hormone‐free
milk was limited.
It is absolutely critical to understand brand parameters
before beginning any creative or product development project.
This is true for retail foods as well as restaurant foods.
Restaurant Foods
It is critical to understand what a restaurant brand stands for.
Chipotle Grill, for example, uses the phrase “food with integ-
rity” to stand for how they source, serve, and stand behind
their food. Unlike most other QSR chains, Chipotle indicates
on their menu the name of the ranch from which they buy
their pork because they are proud to serve meat from a
responsible rancher who treats his animals well. In addition
to polishing Chipotle’s reputation, this also helps create and
build the brand equity of the rancher’s meat, elevating it from
a commodity to a recognized (and highly desired) branded
item. At the retail level, Whole Foods is the best‐known pio-
neer of this kind of social responsibility.
Equally important in developing new items for restau-
rants is a clear understanding of the core customer (discussed
earlier in this chapter). A spicy dish at Chipotle Grill may
appeal to their core customers, but this same level of spice
may be too strong for a McDonald’s or Subway customer, for
example. These subtleties can drive new concept and prod-
uct decisions.
An additional factor when developing restaurant foods is
in‐depth knowledge of the equipment, kitchen layout, and
capabilities of the kitchen personnel. Is the kitchen fully or
only minimally equipped? Are food prep times measured in
minutes, or does time allow preparation of more sophisti-
cated dishes? Are kitchen employees trained culinary profes-
sionals or hourly workers? It is of no value to develop a new
seasoning for deep‐fried applications if your foodservice
client doesn’t use fryers!
Retail Grocery
Large chain retail operations can also strongly influence the
product development process. Most retailers these days
practice “category management,” where managers are given
complete responsibility for everything sold in their “cate-
gory” in the same way the brand managers of food products
manage everything related to their brand. For example, a
Beverage Category Manager is responsible for managing
the retailer’s entire business with regard to carbonated bev-
erages, energy drinks, sports drinks, enhanced waters, and
the like.
Wal‐Mart category managers wield enormous power in
the area of product development. They understand their cus-
tomers well and will often ask suppliers to revise their prod-
uct offering to meet Wal‐Mart’s particular needs in the
category they manage. Due to their enormous purchasing
clout, they usually get what they ask for.
Other retailers set criteria for the products they will accept
on their shelves. Whole Foods Market is the largest chain in
the natural foods industry, with 420 stores as of January 2015.
They make public the list of ingredients they do not accept in
their stores. Thus, a product development project for Whole
Foods should start with a thorough knowledge of this list.
When developing new products, it is important to know
what retailer you are designing your product for because each
has its own idiosyncrasies. In this case, one size definitely does
c01.indd 14 1/14/2016 7:09:38 AM
not fit all. It is much better to know retailers’ individual restric-
tions up front than to have to reformulate a product after it
has been developed, tested, and commercialized.
Strategies for Global
Product Development
The world has become much smaller. The Internet makes it
easy to do business with people all over the world, and rela-
tively low‐cost travel makes it easy for people as well as prod-
ucts to get there—or here, as well as to get products to and
from there.
Many companies source ingredients from afar, whether
they are marketed as such or not. Starbucks has long touted
the unique flavors of coffee beans imported from Ethiopia,
Costa Rica, and Guatemala. Other imported ingredients may
be buried deep in a recipe or formula and can be completely
unknown to the consumer of the product. For example, China
produces most of the world’s garlic, and the United States is
the largest importer of this crop. There is a good chance that
at least some of the garlic in just about anything you’re eating
came from China. The same can be said for most of the B
vitamins and ascorbic acid consumed in this country: They
are mostly manufactured in China.
International trade in food and beverage is big business. It
is highly complex as well. Tariffs and quotas attempt to con-
trol the amount of products that flow from one country to
another. This can put foreign products at a disadvantage to
local options. With the current interest in eating locally
grown food, this is bound to get even more attention.
Trade in food and beverage is also influenced by fluctuat-
ing currencies, which can render foreign goods cheaper or
more expensive depending on the exchange rate on any given
day. The cost of products that must travel great distances,
such as the garlic imported from China, is also affected by the
cost of oil, which fuels transportation, whether by sea, air, or
ground.
Food safety is another huge concern. The 2000s saw sev-
eral food safety problems with food and feed ingredients
imported from China. While China is not the only country to
export tainted products, their rapid economic development
seems to come at the expense of safety controls. As a result,
many companies stopped using ingredients imported from
developing nations.
The risks fall into several categories: insufficient quality
control at the point of manufacture, willful contamination
due to fraud, weak or corrupt government oversight, poor
sanitation practices, opaque distribution chains, and poor
documentation.
On the export side, when a company decides to start mar-
keting products in other countries, it can do so in a number
of ways. The easiest is to simply sell the same product abroad
as at home. This requires no product development or refor-
mulation, unless local restrictions ban the use of certain
ingredients outright due to religious, cultural, or governmen-
tal regulations, but these products will likely require new
labels that meet the language and regulatory requirements of
the new market.
Another option is to adapt an existing product for a for-
eign market. Along with the labeling changes referenced
above, the name may need to be changed and the product
may need to be reformulated. This requires knowledge of the
receiving country’s language, culture, ingredient restrictions,
and taste preferences.
Irish Mist® introduced its drink brand in Germany without
knowing that “mist” is German slang for excrement. A Spanish
potato chip brand called Bum did not sell well in the United
States due to the negative connotations of the name. On the
other hand, a recent Japanese import into the United States
is the ion‐replacement beverage Pocari Sweat®. Despite its
somewhat off‐putting name (at least to many Americans), the
beverage is already available at Amazon.com and is selling
well, at least to its target market of young athletic hipsters.
Cultural differences can also affect product decisions.
A product such as Nestlé’s Beso de Negra (“Kiss of the Black
Woman”), with a scantily clad, buxom woman on the front, is
unacceptable in many Middle Eastern countries, where the
norms are much more conservative when it comes to show-
ing skin. If Nestlé ever decided to market the product in
FIGURE 1.9 A representative selection of the many ingredients banned from foods sold at organic
grocery stores.
acesulfame‐K (acesulfame potassium) BHT (butylated hydroxytoluene) caprocaprylobehenin
acetylated esters of mono‐ and
diglycerides
bleached flour certified colors
ammonium chloride bromated flour cyclamates
artificial colors brominated vegetable oil (BVO) cysteine (l‐cysteine), as an additive
for bread products
artificial flavors calcium bromate dimethylpolysiloxane
aspartame calcium disodium EDTA dioctyl sodium sulfosuccinate (DSS)
azodicarbonamide calcium peroxide dimethylpolysiloxane
Source: http://www.wholefoodsmarket.com/products/unacceptable-ingredients.php
CHAPTER 1 • The Business of New Product Development and the Role of the Culinology® Professional • 15
c01.indd 15 1/14/2016 7:09:38 AM
16 • CULINOLOGY®
the Middle East, they would no doubt have to re‐brand and
re‐label it.
Taste and texture preferences vary widely by geography
as well. Many Asian snacks come in flavors that U.S. con-
sumers consider too “fishy.” If these companies plan to
expand their U.S. presence, they would be wise to develop
more U.S.‐acceptable flavors. Yogurt is another product that
varies widely. In Greece, the product is thick and very tangy.
In Europe, it is thin and tangy. In the United States, many
yogurts are thickened with gums or starches and sweetened
at a much higher level than in Europe. That said, Greek‐style
yogurt has become a huge business in the United States in
the past 5 years.
The best way to ensure success in a new market is to
develop the product from the ground up based on the spe-
cific characteristics of the market. This means starting with
consumer research to identify concept opportunities, flavor
preferences, and market positioning. It is also smart to hire
at least some of your R&D and Marketing staff (or consult-
ants) from the target countries you wish to do business in.
A lot of research and investigation must be done long before
the first product prototypes are developed. The key take-
away is that exporting products is one way to grow a food
business, but it is not as easy as it may look from the shores
of the home country.
The Role of the
Culinology® Professional
Why Consider a Career as a
Culinology® Professional?
A career in food product development can be both chal-
lenging and rewarding. Every sector of the food industry
employs people who perform new product development
(NPD) and NPD‐related tasks. Large industrial concerns
such as Kraft Heinz and General Mills employ hundreds of
highly skilled scientists, many with graduate degrees in gen-
eral food science or in specific areas such as cereal or meat
science. Small concerns might employ one or two part‐time
food scientists.
Chefs working in R&D for retail or foodservice manu-
facturers tend to have culinary rather than scientific back-
grounds. Culinology® program graduates can enjoy the
best of both worlds with integrated training that gives
them the flexibility to practice culinary arts, food science,
or, ideally, both.
The scientific/technical side of product development
requires both theoretical and applied knowledge of chemis-
try, nutrition, government regulations, food safety, sensory
science, food processing, the use of functional food additives,
packaging, and knowledge of the physical properties and
interactions of food ingredients. The culinary side requires
both theoretical and applied knowledge of the principles of
cooking, the culinary use and application of ingredients,
production systems, recipe and formula development, food
trends, equipment operation, and of course, the creativity
and refined sensory skills that come from years of practical
kitchen experience.
Over time, all areas of the food industry will come to value
and benefit from the breadth of knowledge Culinology® pro-
fessionals bring to the workplace. It is too soon to measure
the salary premium they might earn over one discipline or
the other, but Culinology® professionals are both highly
sought after and well compensated.
The work of Culinology® professionals is never dull. Each
day brings new challenges and important decisions that can
affect their employer’s bottom line for years to come. To truly
succeed as a Culinology® professional, one must combine
the creativity, ability to be calm under fire, and passion for
excellence of the chef with the organizational skills, analyti-
cal ability, and attention to detail of the scientist. In addition,
Culinology® professionals are organizationally valuable for
their unique ability to understand the worlds and languages
of chefs and food scientists on large cross‐functional devel-
opment teams. Their experience in both camps can help
them build bridges between the two disciplines.
There’s nothing quite like walking into a national restau-
rant chain or major grocery store and seeing consumers pur-
chasing a product you had a hand in developing. It is truly
rewarding to know that hundreds of thousands, perhaps mil-
lions, of people are experiencing your creation.
The discipline of product development is the true chal-
lenge for Culinology® professionals. It is the real world, where
theories are put to the test and honed into practical experi-
ence. This is the world where business makes, and some-
times loses, millions of dollars introducing and marketing
new food products—and Culinology® professionals are at
center stage.
Considering how large and seemingly sophisticated many
food companies are, you might assume that they would have
product development down cold and turn out one winning
product after another. Nothing could be further from the
truth. The results of the Booz Allen Global Innovation 1000
in 2005 found little correlation between R&D spending at the
world’s largest 1000 companies and the company’s gross
margins.18 Why?
One reason is that very few companies actually employ
the correct product development and marketing methodolo-
gies necessary to succeed in today’s highly segmented and
hyper‐competitive marketplace. Few companies are patient
enough or invest enough, and they do not reward intelligent
risk taking—a key to developing breakthrough products.
Development budgets within food companies are typically
Food
Technology
Culinology
Culinary
Arts
FIGURE 1.10 Culinology® Venn diagram.
Source: Mark Crowell, CRC®.
c01.indd 16 1/14/2016 7:09:38 AM
dwarfed by advertising, promotion, and other marketing
expenditures. R&D spending in the food industry typically
represents only 1 to 2 percent of revenues, compared to 8 to
15 percent of revenues in the high‐tech sector. However,
while an aggressive advertising and promotional campaign
can invigorate even poorly developed products, the results
are typically short‐lived, with little positive impact on com-
pany growth and long‐term profitability.
Most food companies have a marketing focus that also
puts them at a disadvantage. They view product development
as a reactive process rather than a cross‐functional manage-
ment process, where the need to closely link engineering,
operations, procurement, marketing, and R&D activities to
customer requirements is more obvious. As a result, many
food companies have never optimized their product develop-
ment capabilities in a concerted, cross‐functional fashion.
Examples of famous product and food company failures
include Anheuser‐Busch’s Eagle Snacks, Campbell’s Souper
Combos, and New Coke.
Creating and
Managing Product
Development Teams
The most successful product development project includes
members from all disciplines within a company. Culinology®
professionals play a key role in integrating the culinary and
food science disciplines to accomplish project objectives. In
addition, it is important to have a strong Project Manager to
pull all members of the project team together. The following
list of disciplines includes those most directly involved in a
product development team.
Project Manager
The Project Manager’s responsibilities are to communicate
and uphold the project’s objectives and to keep the team
members on strategy and on time. Perhaps more important,
though, the Project Manager provides leadership.
Successful Project Managers will encourage the team to
work together in a cooperative, mutually beneficial manner
by creating a culture of openness. They ensure that the team
members share a common understanding of the project goals
and a common language. This is where a Culinology® profes-
sional can be especially valuable.
Culinology® professionals should possess the ability to
speak the language of culinary arts as well as the language
of the scientist. The ability to bridge this gap in everyday
language makes Culinology® professionals extremely valu-
able as well as appropriate for overall project management
responsibility.
Product Developer
There should be one person on the team who has primary
responsibility for the development of the actual food proto-
types. If the project is highly technical in nature, this person
may be a Food Technologist; if it is largely culinary, it may be
a Chef. However, it is always a good idea to have a number of
Culinology® professionals working together on the project.
After one person develops a prototype, he or she should have
other team members evaluate it and then make revisions,
again and again, until it is ready to show to the Project
Manager and any other project stakeholders. Early hand‐
made prototype development is usually referred to as bench-
top development because much of it occurs at the lab bench of
a developer.
Consumer Insights
The discipline of consumer research, more recently called
consumer insights, is imperative to the process. Many small
companies forgo spending any of their small budget on
consumer insights, but this is often a mistake. It is much
more costly to develop and launch a product that fails
than to spend money on gaining consumer insights up
front. A frugal manager with consumer insights responsi-
bility may learn things in unconventional ways—for exam-
ple, through polling friends and family, doing online
research, and secondary research from sources such as
newspapers, books, the Internet, and so on—all of which
can be less expensive than traditional research. Regardless
of the approach or budget, any consumer insight is better
than none at all.
Packaging
Regardless of whether the project is for retail packaged food
or a restaurant menu item, there is a packaging component
that must be considered during product development. The
packaging person may be a designer, engineer, or a supply or
sourcing specialist. The key responsibilities of this person are
to assure that the chosen package supports the requirements
of the processing parameters, shelf life, safety, distribution,
and marketing of the product.
Commercialization
At least one member of a product development team should
be responsible for making sure that the concept is feasible
from a food safety, sensory quality, economic, and manufac-
turability perspective. This person should also be heavily
involved in the development of the product during the early
phases of the project to save the development team from pur-
suing unrealistic ideas while at the same time gaining famili-
arity with the “Gold Standard”: the target product to be
commercialized. It is critical that this person be able to mini-
mize the differences in product quality that inevitably occur
during scale‐up and production.
The commercialization team is also responsible for
identifying appropriate manufacturing processes and then
finding the facilities and equipment required to produce
the product. This may be in a company‐owned facility or in
one outside the company under a contract manufacturing
(co‐packing) arrangement.
CHAPTER 1 • The Business of New Product Development and the Role of the Culinology® Professional • 17
c01.indd 17 1/14/2016 7:09:38 AM
18 • CULINOLOGY®
Regulatory
As the product is being developed, a number of regulatory
issues must be addressed, including restrictions on what the
product can be called. For example, the USDA specifies what
qualifies to be labeled as “Beef Chili” versus what needs to be
labeled “Chili with Beef.” The FDA mandates an entire list of
food “Standards of Identity” that define a food’s composition
by the specific ingredients (and amounts of ingredients) it
must contain in order to be labeled as that food.
The regulatory role on a food product development team
also includes overseeing the development of the nutrition
facts panel and ingredient declaration, both required by law.
Depending on the product, other regulatory issues may per-
tain, such as getting process approval from the FDA on low‐
acid, shelf‐stable products, and label review will have to be
conducted by the USDA in most cases if the product contains
meat or poultry.
Conclusion
The business of new product development offers challeng-
ing, technically demanding careers for Culinology® profes-
sionals that feature opportunities in almost every food
business segment. Culinology® professionals may find them-
selves creating organic frozen meals from ethically sourced
ingredients, designing the next hit snack food, or developing
new QSR sandwiches that will be consumed by millions of
people. The opportunities are enormous in this ever‐ evolving
landscape of food technology, trends, and consumer tastes,
and the Culinology® professional is uniquely positioned to
take advantage of them
1 J. M. Keynes, A Treatise on Money (New York: AMS Pr. Inc. , 1976).
2 B. Jaruzelski, “The Booz Allen Hamilton Global Innovation 1000:
Money Isn’t Everything,” Strategy+Business 7, no. 41 (2005), retrieved
November 15, 2009, from http://www.ausicom.com/filelib/PDF/Research
Library/Booz%20Allen%20Hamilton%20Innovation‐1000 .
3 R. G. Cooper, Winning at New Products: Accelerating the Process from Idea to
Launch, 3rd ed. (New York: Perseus Books Group, 2001).
4 R. Vernon, “International Investment and International Trade in the
Product Cycle,” in The Quarterly Journal of Economics (Cambridge: MIT
Press, 1966).
5 ERS/USDA, “Briefing Room: Food CPI and Expenditures,” Measuring the
ERS Food Expenditure Series, retrieved November 16, 2009, from http://
www.ers.usda.gov/Briefing.
6 “U.S. Monthly Advanced Retail Sales Survey, August 2009,” U.S. Census
Bureau News, retrieved from http://www2.census.gov/retail/releases
/historical/marts/adv0908 .
7 Food Marketing Institute, “Facts and Figures,” Supermarket Facts
(May 1, 2008), retrieved November 15, 2009, from http://www.fmi.org
/facts_figs/?fuseaction=superfact.
8 Trader Joe’s, http://traderjoes.com/our‐story/timeline, 2013.
9 Food and Drug Administration, “Guidance for Industry: Frequently Asked
Questions about GRAS,” December 2004, retrieved from http://www.fda.gov
/Food/GuidanceRegulation/GuidanceDocumentsRegulatoryInformation
/ucm061846.htm.
10 M. Moss, “Peanut Case Shows Holes in Food Safety Net,” New York Times:
Breaking News, World News, and Multimedia, February 9, 2009, retrieved
November 15, 2009, from http://www.nytimes.com/2009/02.
11 “Politics of the Plate,” Food Safety Lapses: Food Politics, July 8, 2008, retrieved
November 15, 2009, from http://www.gourmet.com/foodpolitics.
12 C. Lee, “FDA Safety Lapses: The Food Times,” All‐Consuming News, March
18, 2008, retrieved November 15, 2009, from http://www.thefoodtimes
.com/2008.
13 M. Neumann and N. Fortin, eds., Food and Drug Regulation: A Web Book of
Student Papers, on file with the Institute for Food Laws and Regulations at
Michigan State University.
14 “About FSIS,” USDA Food Safety and Inspection Service Home, n.d., retrieved
November 15, 2009, from http://www.fsis.usda.gov/about_fsis.
15 “Food for Thought VI,” Diet, Nutrition, and Food Safety News, December
2005.
16 NPD, National Eating Trends® (NET®).
17 Jeremy W. Peters, “In Small Packages, Fewer Calories and More Profit,”
New York Times, July 7, 2007.
18 http://www.boozallen.com/media/file/Global_Innovation_1000_2006 .
c01.indd 18 1/14/2016 7:09:38 AM
CHAPTER 2 • The Principles of Food Science • 19
Lead Author: Lisa J. Mauer, Ph.D., Professor, Department of Food Science , Purdue University
M. Fernanda San Martin-Gonzalez, Ph.D., Associate Professor, Department of Food Science ,
Purdue University
2
The Principles of
Food Science
Introduction
To create high‐quality foods, it is important to understand
the basic structures and functions of food ingredients, includ-
ing both macro and micro nutrients, their interactions, how
cooking techniques influence these, and ultimately their
physical and chemical stability in foods. This chapter
introduces the structures of water, proteins, lipids, and car-
bohydrates, otherwise known as the “building blocks” of
foods. Emphasis will be on the types of solids, liquids, and
gases; water‐solid interactions; phase transitions; and chemi-
cal reactions in foods. Because most cooking techniques
involve heat interacting with foods, understanding the effects
of temperature on food component structures and reactions
is essential. The types of heat, thermal properties, and heat
transfer will be described before addressing culinary cooking
techniques from the chef ’ s viewpoint in the following
chapter.
The Nature of Matter
Food matrix, intermediate‐moisture food, amorphous food
components—sometimes it sounds as if food scientists can-
not possibly be talking about the foods everyone eats. Why
use these terms instead of more simply describing a soft
food? The reason is that food scientists seek to understand
the fundamental nature of food so that they can control and
predict what will happen to food when it is formulated,
cooked, packaged, and stored. Because prediction is useful
only when it is precise, the study of food requires the precise
language of science.
The first challenge is simply to describe the way foods
occupy space. Matter exists as solid, liquid, or gas, but many
foods are combinations of two different phases of matter,
which is why it is hard to say whether jelly is a solid or a liq-
uid, or beer foam a liquid or a gas. In other words, foods are
complex physical structures that are best described as disper-
sions . Dispersions are systems of discrete particles of one
phase of matter suspended or dispersed within a second con-
tinuous phase of matter. Dispersions are characterized by
having both continuous phases (usually the majority ingredi-
ent that serves as a matrix) and discontinuous or “dispersed”
phases (the discontinuous phase is incorporated within the
continuous phase). The amounts and types of solids, liquids,
and gases, and their location within these two phases, influ-
ence the ultimate texture, stability, and accep t ability of food
products. For example, a dispersion with water as the con-
tinuous phase and fat as the discontinuous phase dispersed
within it (such as cream) has much different properties than
a dispersion with a solid fat as the continuous phase and
water as the discontinuous phase (such as refrigerated but-
ter). Creating dispersions from the macro‐ and micronutri-
ents that comprise foods is much like constructing a building
in which the walls, floors, and ceilings form the continuous
phase and everything within the “rooms” is the dispersed
phase. The combinations are endless.
The ways that the basic functional (“reactive”) groups of
atoms in food dispersions interact with each other deter-
mines the structure, texture, and stability of foods as well as
how they react to cooking. For example, both gelatin and egg
white proteins can create gels— structures with a continu-
ous solid phase (the protein gel matrix) and a dispersed liquid
phase (the water trapped within the protein matrix)—but
each responds differently to heat. An initial dispersion of gel-
atin proteins in water (called a sol , a solid dispersed in a liq-
uid) will become a gel (like Jell‐O®) with a continuous protein
matrix containing a discontinuous water phase after heating
and cooling. This type of gel is thermo‐reversible —that is, it
will liquefy when heated and reforms as a gel when chilled.
Egg whites, on the other hand, form irreversible gel structures
when heated that are unaffected by cooling and actually
toughen with reheating. Therefore, the strength of the
c02.indd 19 1/22/2016 12:53:28 PM
20 • CULINOLOGY®
FIGURE 2.1 Classification of food dispersions by types of continuous and dispersed phases. Most
foods are dispersions, and examples of each type of dispersion are provided.
Continuous
Phase
Dispersed
Phase
Descriptive Words for Each
Type of Dispersion Food Examples
Liquid Gas Foam, froth, bubble, air,
carbonated liquid
Whipped egg white, whipped cream, champagne, soda
Liquid Liquid Emulsion Milk, cream, salad dressing, mayonnaise, cream soup,
many sauces (béchamel, hollandaise)
Liquid Solid Suspension, sol, colloidal
dispersion
Eggnog, chocolate milk, coffee, herbs suspended in
sauces and dressings
Solid Gas Foam, suspension Marshmallow, leavened bread, nougat, ice cream, angel
cake, meringue
Solid Liquid Gel Gelatin gels (Jello®), custard, cooked egg, tofu
Solid Solid Solid Toffee, chocolate, noodles, herbs in cheeses and butters
Source: L. Mauer and F. San Martin-Gonzalez.
CI
–
Na
+
Van der
Waals
Common Molecular Interactions in Food
Hydrophobic
Hydrogen
Bond
Dipole-dipole
Ionic and
Ion-dipole
Covalent
Weak attractive and
repulsive forces
between neutral
molecules.
Attractive forces
between “water-
fearing” molecules,
such as fatty acid
hydrocarbon chains,
that lead to self-
aggregation in aqueous
environments.
The relatively weak
attraction between a
partial-positive charge
on a hydrogen atom
and a partial-negative
charge on another
atom, typically N or O in
foods.
Electronic attractive or
repulsive interactions
between functional
groups of opposite
charge; electronic
opposites always
attract.
Ionic bonds are strong
electronic bonds
between charged atoms
or molecules. Solid ionic
compounds form stable
crystal structures (salts).
Water easily dissolves
ionic compounds.
Extremely strong
interaction between
atoms caused by a
shared pair of electrons.
Covalent bonds break at
temperatures above
that of boiling water.
Van der Waals
attractive forces
between fatty acids are
responsible for the
crystallization of lipids.
When fats are heated,
the heat energy can
disrupt the Van der
Waals attractions, at
which point the lipids
melt (e.g., melting of
butter and chocolate).
R
R
O O
O
R R
N
N
H
H
O
O R
HO
O
O
O
a
a
a
R
R
NH
3
H H
H
H
C
H
H
H HC
R
H
N
H
HO
O
O
O
R
Hydrophobic
interactions are a
driving force for the
separation of mixtures
of hydrophilic and
hydrophobic
ingredients (emulsions),
such as salad dressings
and mayonnaise, in the
absence of an
emulsifier.
Water interacts with
itself and other
compounds via
hydrogen bonds. The
solubility of many
ingredients and the
crystallization of sugar
are governed by
hydrogen bonding.
The structural changes
of proteins in response
to changing pH are a
result of changing
dipole-dipole
interactions between
amino acids, as is seen
when milk curdles upon
acidification.
Ionic interactions are
responsible for the
crystallization of salt
and the gelation of ion-
setting gels (such as
sodium alginate in the
presence of calcium
ions).
Covalent interactions
(such as glycosidic
linkages and amide
bonds) are responsible
for the formation of
polymer structures
(carbohydrates and
proteins, respectively).
Covalent crosslinks
between polymers form
very strong gels, such as
those established with
transglutaminase or
disulfide linkages.
+ –
–
+ –
–
–+
+
+
FIGURE 2.2 Common interactions in foods responsible for structure, function, stability, and resistance
to external forces applied during food production (such as mixing and cooking), organized in order
of increasing strength.
Source: J. Sanchez and L. Mauer.
c02.indd 20 1/14/2016 7:11:28 AM
CHAPTER 2 • The Principles of Food Science • 21
FIGURE 2.3 Common functional groups
(sequences of atoms) found in the building blocks
of foods (carbohydrates, proteins, and lipids). The
types, locations, and sequences of these functional
groups in the building blocks of foods govern the
structure, function, texture, and reactivity of foods.
The use of “R” is an abbreviation for carbon‐
containing molecules attached to the identified
structure.
Functional Group Definition Structure
Presence in the
Building Blocks
of Food
Methyl
A methyl group is a
carbon atom bound
to three hydrogen
atoms. It is
hydrophobic.
Carbohydrates,
lipids, and proteinsR
CH3
Hydroxyl
A hydroxyl group is
an oxygen atom
bound to a hydrogen
atom. It is hydrophilic.
Carbohydrates and
proteins
Sulfhydryl (Thiol)
A sulfhydryl or thiol
group is a sulfur
atom bound to a
hydrogen atom. It is
somewhat
hydrophilic.
Proteins
Carbonyl
A carbonyl group is a
carbon atom double
bonded to an oxygen
atom. Additional
bonds to the carbon
determine the nature
and name of groups
that contain the
carbonyl functional
group.
Carbohydrates,
lipids, and proteins
R
OH
R
SH
O
C
Aldehyde
An aldehyde group is
a carbonyl group with
one bond to carbon
and one bond to
hydrogen.
Carbohydrates
O
R H
Ketone
A ketone group is a
carbonyl group with
two bonds to carbon.
Carbohydrates
O
R1 R2
Carboxyl
(Carboxylic acid)
A carboxyl, or
carboxylic acid group,
is a carbonyl group
with one bond to
carbon and one bond
to a hydroxyl group.
Proteins
Ester
An ester group is a
carboxylic acid group
where the oxygen-
hydrogen bond is
replaced with an
oxygen-carbon bond.
Lipids
Amide
An amide group is a
carbonyl group with
one bond to carbon
and one bond to
nitrogen.
Proteins
Disulfide
A disulfide group is a
pair of sulfur atoms
with two bonds: one
to each other and one
to a carbon atom.
Proteins
O
R OH
S
SR1
R2
O
R1
R2
O
O
R1
R2
N
H
Source: J. Sanchez and L. Mauer.
intermolecular bonds holding the egg protein gel together
must be stronger, or at the very least different, from the bonds
creating the gelatin gel. In fact, gelatin gels are stabilized by a
combination of hydrogen bonding and hydrophobic interac-
tions that are about 10 times weaker than the combined bond
strengths of the hydrogen and disulfide bonding that stabilize
egg protein gels. Understanding the structures of food com-
ponents, including the types of elements and bonds of which
they are built, is an important first step in understanding and
creating food texture.
Types of Solids
The previous section discussed how the physical structures
of foods are dispersed systems composed of different states
of matter. Dispersions describe the large‐scale properties of
foods but not the microscopic and molecular level composi-
tion of foods. This section dives down to the atomic level to
explain the chemical, not physical, composition of food.
Ultimately, the chemical forces and attractions at the molec-
ular level influence how all matter behaves at the visible
physical scale.
The solid building blocks of foods are carbohydrates,
fats (fats are lipids that are solid at room temperature, while
oils are lipids that are liquid at room temperature), and
proteins. At the molecular level, these compounds are com-
posed mainly of atoms of carbon, oxygen, and hydrogen.
The atoms are covalently bound, which means that they
share electron pairs to form chemical bonds between each
other in characteristic sequences. These sequences form
functional groups of atoms, and the nature of these func-
tional groups gives each of the three building blocks their
unique set of appearances, structures, functions, and
reactivity.
Bond or interaction energies (the strength with which a
chemical bond or interaction holds two atoms together) are
important in determining the effects of cooking on the food
structure. The type and amount of energy (from heating or
physical work such as beating, whipping, shaking, etc.)
determines which, if any, bonds are disrupted. Weaker bonds,
such as hydrogen bonds, are more easily disrupted than
stronger bonds, such as covalent bonds or ionic bonds. Egg
whites are a good example. Egg whites beaten to stiff peaks
have had their proteins gently unfolded by physical shear.
Their exposed hydrophobic centers trap air and form small
bubbles to create a foam. On the other hand, egg whites
cooked on the stove have had their proteins highly disrupted
by heat. The disrupted hydrogen bonds denature the pro-
teins and cause them to bind together in a continuous phase,
stabilized by disulfide covalent bonds, to form a gel. The
types of bonds present in the food ingredients, as well as
those developed during food preparation and cooking, in
turn influence the structure, reactivity, and texture of each of
the components in the food. They also affect the sensory
qualities (appearance, taste, aroma, texture) of the finished
product.
c02.indd 21 1/14/2016 7:11:28 AM
22 • CULINOLOGY®
Carbohydrates
Carbohydrates are composed of carbon, hydrogen, and oxygen
in the following ratio: (C•H
2
O)
n
, where n is ≥ 3. The chemical
formula gives sense to the name: With one molecule of water
for each atom of carbon, carbohydrates are essentially
hydrates of carbon. Each carbon atom in a carbohydrate is
bound to an oxygen atom, typically as a hydroxyl group but
sometimes as a carbonyl. If fully digestible, carbohydrates
contribute 4 kcal/g to foods. Monosaccharides, the basic
building blocks of carbohydrates, are by definition the sim-
plest (and smallest) carbohydrates. They are classified by the
location of the carbonyl group, which makes them either alde-
hydes or ketones. A carbonyl group is composed of a carbon
atom double‐bonded to an oxygen atom: C O. When this
group is found at the end of a molecule’s carbon backbone, the
molecule is an aldehyde. When this group is found in the mid-
dle of a molecule’s carbon backbone, the molecule is a ketone.
Most of the major monosaccharides used as food ingredients
are aldehydes. The only major monosaccharide used as a food
ingredient that is a ketone is fructose.
Common monosaccharides used or found in foods, such
as glucose and fructose, have a C
6
H
12
O
6
structure and are called
hexoses because they both contain 6 carbon atoms. The dif-
ference in the carbonyl location between glucose (aldehyde
group) and fructose (ketone group) contributes to the greater
hygroscopicity (affinity for binding water) and lower melting
point of fructose. Because they both have a C
6
H
12
O
6
composi-
tion but a different location of the carbonyl group, glucose
and fructose are isomers of each other—that is, they have
the same chemical formula but different arrangement of the
atoms. Isomerization reactions, such as those driven by
the enzyme glucose isomerase, can be used to convert glucose
into fructose, which is widely used in the later stages of the
production of high‐fructose corn syrup from starch (a poly-
mer or long chain of glucose subunits). In addition, all mono-
saccharides can participate in non‐enzymatic browning
reactions (Maillard browning and caramelization), and they
are called reducing sugars because their ring structures are
able to open while in solution to expose the reactive carbonyl
group. The many –OH (hydroxyl) groups present in the mono-
saccharide structure enable hydrogen bonding with water,
making them highly water soluble.
In nature, carbohydrates are rarely found in their simple
monosaccharide form. Simple sugars are sweet and scarce,
which is why animals and humans alike treasure their com-
mon sources: the fruits of plants and the nectar of flowers.
The carbonyl functional group allows monosaccharides to
act as building blocks that can join together via glycosidic
linkages (covalent bonds). For the most part, monosaccha-
rides in nature combine ( polymerize) to form disaccharides
(2 monosaccharide units), oligosaccharides (2 to 10 units),
and polysaccharides (more than 10 units; some contain as
many as 15,000). Polysaccharides are by far the most abun-
dant carbohydrate structures in nature—they will be dis-
cussed later on.
Disaccharides, like monosaccharides, are rare in nature and
also tend to be sweet. Common disaccharides include sucrose
or table sugar (glucose+fructose), maltose (glucose+glucose),
and lactose (glucose+galactose). Disaccharides vary in sweet-
ness, hygroscopicity, solubility, crystallization tendency, and
reactivity, depending on how their monosaccharides are bound
together. For example, sucrose is not a reducing sugar and can-
not participate in Maillard browning because of its inability to
expose a carbonyl group while in solution, while maltose and
Food Component Functions
Water Hydration, solvent or dispersion medium, reaction medium, heat transfer medium, mobility,
hygro scopicity, softness, basic part of the structure (“bound” water) and/or main constituent of the
food, polar phase, surface or interfacial tension, vapor pressure or water activity
Protein Structure, texture, viscosity, water binding or water retention, heat denaturation, coagulation, gela-
tion, cohesion, adhesion, elasticity, emulsification, foaming, fat and flavor binding, nonenzymatic
Maillard browning for color and flavor development, nutritional source of essential amino acids
Sugars (mono- and
disaccharides)
Sweeteners, structure, texture, solubility, crystallization controllers, control of a
W
, Maillard (nonen-
zymatic) browning, caramelization, humectants, bulk, viscosity, fermentation source
Polysaccharides
(starches, gums)
Thicken, improve texture, form gels, inhibit crystallization, whipping agents, stabilize foams, stabilize
emulsions, binders, stabilize proteins, encapsulate, flocculate/clarify, processing aids, provide spe-
cific rheology, inhibit syneresis, stabilize suspensions, dispersing agents, to bind/hold water, to form
films and coatings
Lipids Texture, structure, mouthfeel, flavor, satiety, medium of heat transfer, tenderizing agent, carrier of fat-
soluble vitamins ADEK, nutritional source of essential fatty acids (linoleic, linolenic), development of
rancidity (hydrolytic or oxidative), emulsion formation and stabilization, delivery agent of lipophilic
flavors, nutrients, and colors
FIGURE 2.4 Functions of water, proteins, carbohydrates, and lipids in foods.
Source: L. Mauer and F. San Martin-Gonzalez.
c02.indd 22 1/14/2016 7:11:28 AM
CHAPTER 2 • The Principles of Food Science • 23
OH
OH
OH
OH
OH
branch point
Glycosidic linkage
O
O
O
O
O
O
HOGlucose
Amylose
(linear polymer of glucose subunits
connected by glycosidic linkages)
Amylopectin
(branched polymer of glucose subunits
connected by glycosidic linkages)
hydrolysis
Starch
polymerization
HO
HO
H
H
H
H
H
H
H
H
H
OH
OH
O
O
O
HO
HO
H
H
H
H
OH
OH
OH
O
O
HO
H
H
H
H
O
O
O
O
HO
OH
CH2OH
CH2OH
CH2OH
CH2OH
O
HO
OH
O
O
HO
OH
O
O
HO
OH
FIGURE 2.5 The basic structures of common carbohydrates: the monosaccharide glucose and the
polysaccharide components of starch (amylose and amylopectin).
Source: L. Mauer.
c02.indd 23 1/14/2016 7:11:28 AM
24 • CULINOLOGY®
lactose are reducing sugars and can participate in Maillard
browning. Enzymes can be used to hydrolyze (break apart with
water) complex carbohydrates into their monosaccharide
components to alter sweetness, viscosity, and other properties
in a food product. One example is the use of the invertase
enzyme in chocolate‐covered cherries to hydrolyze the disac-
charide sucrose (a crystalline solid) into glucose and fructose.
The enzyme hydrolysis creates a sugar syrup inside the choco-
late coating after the candies are formed because glucose and
fructose have weaker crystallization tendencies (and a lower
mutual deliquescence point) than sucrose.
Polysaccharides constitute more than 90 percent of the
carbohydrate biomass found in nature; they form the rigid
polymer structures that give plants their shape. Uncooked,
they are tough, bland, and hard to digest. In purified form,
they are added as ingredients to most processed food
products. Examples of polysaccharides include starch, cellu-
lose, hemicellulose, pectin, glycogen, chitin, inulin, gums
(guar, locust bean, alginate, agar, carrageenan, etc.), and
other classes of dietary fiber. The functions of polysaccha-
rides reflect the diversity of their many sizes and structures.
At the molecular level, the many hydroxyl groups along
polysaccharide polymer structures facilitate hydrogen bond-
ing with water molecules. Most polysaccharides have the
ability to thicken solutions due to their long polymer struc-
tures, which entangle as a solution is stirred; their long struc-
tures entrap large volumes of water within their sweeping
volume (radius of gyration). In addition to thickening, some
polysaccharides are able to form gels if the conditions are
right for intermolecular interactions between them, leading
to a polysaccharide continuous phase in the dispersion. For
example, the gel formation ability of the polysaccharide
sodium alginate in the presence of calcium ions is the basis
for many spherification applications.
Starch is found in discrete particles (starch granules) in
nature and is the major form of energy stored in higher plants
(seeds, roots, fruits, leaves, etc.). Starch is used in foods for
multiple purposes, including thickening, gel formation, stabi-
lizing suspensions, etc. Starch is made of amylose (a linear
polysaccharide of repeating glucose subunits) and amylo-
pectin (a branched polysaccharide of glucose subunits). In
general, amylose makes up about 30 percent of the starch
structure and amylopectin makes up 70 percent, but the ratio
is strongly dependent on the plant source. These linear and
branched structures exhibit different physical and functional
properties. In starch granules, the amylopectin structures
orient out radially from the core (hilum) and create rings of
crystalline and amorphous regions. The branched areas of
amylopectin molecules create the amorphous regions in the
starch granule. The hard outer crystalline structure of native
starch granules results in their insolubility in cold water. To
obtain improved functionality from native (unmodified)
starch (beyond dusting on surfaces to prevent sticking or
using as an anticaking agent), its granular structure must be
disrupted by processing (adding moisture, heat, enzymes,
etc.) to alter its structure, chemical, and/or physical proper-
ties. To enhance or alter native starch functionality, modified
starches were developed to reduce the energy required to
cook them or to modify their cooking characteristics; impart
cold water swelling or increased solubility; increase or
decrease paste viscosity or gel formation and strength;
enhance clarity; increase resistance to shear; increase freeze‐
thaw and pH stability; enhance interactions with other ingre-
dients (for example, lipids); and reduce gel syneresis or
breakdown. Frequently, starch is heated above the melting
temperature of its crystalline structures to enhance its water
solubility and functionality in foods, either by the chef when
using a native starch or starch‐containing ingredient (such as
wheat flour) or by the modified starch manufacturer during
the ingredient production process.
Many types of non‐starch polysaccharides are used as
food ingredients to take advantage of their thickening,
stabilizing, and/or gelling abilities. Examples of non‐starch
polysaccharides include pectins, plant seed gums (guar gum,
locust bean gum), plant exudate gums (gum Arabic, gum
tragacanth), seaweed gums (agar, carrageenans, and algi-
nates), bacterial gums (xanthan gums, gellan), and cellulose
gums (methylcellulose, carboxymethyl cellulose, microcrys-
talline cellulose). Unlike starch, many of these polysaccha-
rides contain more monosaccharide types than simply
glucose. Whole books are dedicated to the structure‐function
relationships of these polysaccharides, encompassing their
unique properties, textures, typical use conditions, synergies,
promoters, inhibitors, viscosities, and so on. This breadth of
functional traits enables a chef to create unique formulations
targeted to specific applications, whether they be a spherified
sodium alginate–based gel, a thermo‐reversible soft elastic
methylcellulose gel that uniquely thickens as it is heated and
thins as it is cooled, or a stable quick‐acting guar gum used as
a thickener in cold and hot water applications.
Lipids
Lipids, like carbohydrates, are composed of carbon, oxygen,
and hydrogen, but most of the carbon atoms in lipids are
bound only to other carbon atoms and to hydrogen. Without
many oxygen atoms or hydroxyl groups, lipids are unable to
hydrogen‐bond with water. This makes them mostly insolu-
ble in water but soluble in organic solvents like other oils.
Lipids provide the most concentrated source of energy
(9 kcal/g) of the food solids, essential dietary fatty acids (lin-
oleic and alpha‐linolenic acids), and are thought to contribute
to the feeling of satiety (fullness) after eating.
Food lipids are classified as either oils or fats depending on
their physical form at 68°F (20°C): Oils are liquid, and fats are
solid. Oils typically come from plant sources (except for fish
oils), and fats typically come from animal sources (except for
coconut and palm kernel oils). Fats form crystal (physical)
structures, and their melting points govern their stability and
texture in foods.
Lipids in foods are most often triglyceride structures
comprised of a glycerol backbone with three fatty acids
bound to it via ester bonds. Factors that influence the func-
tionality of lipids include the types, ratios, and locations of
c02.indd 24 1/14/2016 7:11:28 AM
CHAPTER 2 • The Principles of Food Science • 25
the fatty acids on the triglyceride backbone (including fatty
acid chain length [number of carbons], level of saturation,
number and location of double bonds, and whether they have
a straight “trans” or kinked “cis” structure); the sources of the
lipids (soybean, olive, lard, cocoa, etc.); the ratio of solid to
liquid fractions at a given temperature; treatment of the lipids
by hydrogenation or interesterification; the conditions used
during chilling and tempering; the type and quantity of emul-
sifiers (if any); the degree of oxidative or hydrolytic rancidity;
the smoke point; and the shipping and storage conditions.
The fatty acids that make up lipids are classified by the
number of carbons (chain length between 4 and 24 carbons)
and the presence and location of double bonds along this
chain. Double bonds are areas of unsaturation because the
carbon atom is not fully saturated with hydrogen atoms.
Saturated fatty acids contain no carbon‐carbon double bonds
(C C), and unsaturated fatty acids contain at least one car-
bon‐carbon double bond. Monounsaturated fatty acids, such
as oleic acid, contain one carbon‐carbon double bond, while
polyunsaturated fatty acids, such as linolenic acid, contain
more than one carbon‐carbon double bond.
The linear structure of saturated fatty acids allows for
closer chain packing in the triglyceride structure, which
results in higher melting temperatures and greater likelihood
that lipids containing more saturated fatty acids will be solid
at room temperature than lipids containing more unsaturated
fatty acids. The closer packing of straight fatty acids increases
the van der Waals attractive forces between the fatty acids
and provides greater resistance to melting—that is, more heat
energy is required to destabilize them. Fats used for baking
must have high melting points to impart the desired function-
ality to bakery products, such as laminating the dough and
entrapping steam to leaven croissants or puff pastries.
Therefore, baking fats contain a high concentration of satu-
rated fats and/or trans fats. Solid products such as chocolates
also contain saturated fats. Saturated fats (and cholesterol, a
waxy substance found only in animals, including seafood)
have been linked to negative health effects, including heart
disease, obesity, and other illnesses, and must be declared on
the nutrition facts panel on food labels. While all fats and oils
are in reality made up of mixtures of saturated and unsatu-
rated fatty acids distributed among the triglyceride “back-
bones,” animal fats (except for fish oils) consist predominantly
of saturated fatty acids, while vegetable oils are predominantly
made up of unsaturated fatty acids (except for tropical oils
such as coconut and palm kernel oil).
Unsaturated fatty acids can be classified as cis or trans
based on the location of the hydrogen atoms attached to each
Linear, crystalline
regions
Branched,
amorphous
regions
Starch granule Amylopectin
Crystalline hard shell
Source: L. Mauer.
FIGURE 2.6 The structure of a native starch granule.
H
H
Glycerol Individual fatty acids
H R1OC C
O
H R2OC C
O
H R3OC C
O
Source: L. Mauer.
FIGURE 2.7 Lipids: triglyceride structure. The
R groups on the triglyceride structure represent
individual fatty acids.
c02.indd 25 1/14/2016 7:11:29 AM
26 • CULINOLOGY®
of the paired carbons bound together by a double bond. Most
natural sources of lipids contain cis unsaturated fatty acids
that have the hydrogens on the same side of the C C double
bond, creating kinks in the linear structure. These kinks limit
the closeness of fatty acid chain packing, thus reducing the
melting point of the lipid (that is, less heat energy is required
to destabilize them because there are fewer van der Waals
attractive forces between the fatty acids). As the number of
double bonds in a fatty acid increases, the structure becomes
more kinked and the melting point decreases. That is why
polyunsaturated fatty acids (containing more than one double
bond) have very low melting temperatures (below room tem-
perature) and are therefore liquid at room temperature. On
the other hand, most trans fatty acids are created syntheti-
cally via hydrogenation and have hydrogens on opposite sides
of the C C double bond, creating a more linear structure that
is similar to saturated fatty acids. The more linear trans struc-
ture allows partially hydrogenated vegetable shortenings to
mimic the functionality and melting points of saturated fats
such as lard, but without the presence of cholesterol. Concerns
have been raised about the negative health effects associated
with consuming trans fats, and since 2006 trans fats must be
declared on the nutrition facts panel of all packaged foods.
Hydrogenation is a reaction between hydrogen gas and
unsaturated fatty acids in the presence of catalysts that ulti-
mately results in a more saturated fatty acid. This is how
unsaturated liquid oils are transformed into saturated (or
partially saturated) solid fats and shortenings. Important
uses of hydrogenation include control of physical and chemi-
cal properties, increasing the melting temperature of a lipid,
enabling a cheaper oil to mimic more expensive fats, creating
unique functional characteristics, and increasing the shelf
life or stability of a liquid oil by saturating most of its highly
re active C C double bonds, thereby reducing its tendency to
undergo oxidative rancidity. As an intermediate of the hydro-
genation reaction, trans fatty acids are produced, and par-
tially hydrogenated lipids often contain trans fats. These
trans fats have straighter structures than the initial cis
Structure
Stearic 18 0
(saturated)
Oleic 18
OH
O
Linoleic 18
18
HO
O
O
OH
Fatty Acid
Common
Name
Number of
Carbon
Atoms
Number of
Double Bonds Typical Fat Source
In most fats and oils, although
it is more prevalent in animal
fats than most plant fats (ex-
cluding cocoa butter and shea
butter)
1
(monounsaturated)
Although present in many
animal and vegetable lipids,
oleic acid is most commonly
associated with olive oil.
2
(polyunsaturated)
An essential omega-6 fatty
acid found in nuts, seeds, and
vegetable oils.
Alpha-Linolenic 3
(polyunsaturated)
An essential omega-3 fatty
acid found in seeds, nuts,
and notably soybean and
canola oils.
OH
O
FIGURE 2.8 Common saturated and unsaturated fatty acids containing 18 carbons. The hydrogenation
process can be used to convert fatty acids containing the same number of carbons from lower to higher
degrees of saturation (for example, linolenic acid to linoleic acid, linoleic acid to oleic acid, and so on).
Source: L. Mauer.
c02.indd 26 1/14/2016 7:11:29 AM
CHAPTER 2 • The Principles of Food Science • 27
structures and therefore have increased melting tempera-
tures. A fully hydrogenated lipid does not contain trans fats;
when the hydrogenation reaction is taken to completion, all
fatty acids are converted into saturated structures.
Interesterification is used as an alternative to hydrogena-
tion in order to solidify oils without trans fat formation.
Interesterification is the process by which fatty acids are
rearranged on the glycerol backbone to achieve desired
performance characteristics. Because lipid function is deter-
mined by the types of fatty acids and their distribution on
the glycerol backbone, interesterification can be used to
modify or create new triglycerides to improve consistency
and usefulness of the fats or oils for targeted applications by
replacing unsaturated fatty acids with more saturated ones
on the glycerol backbone, or rearranging the fatty acid loca-
tions on the glycerol backbone to change triglyceride pack-
ing structures. The process can be conducted enzymatically
or with chemical catalysts. However, even though it is now
possible to create vegetable oil‐based shortenings without
trans fats, these methods are more expensive than
hydrogenation.
Rancidity, or off‐flavor development in lipids, is caused by
hydrolytic or oxidative reactions. Hydrolytic rancidity involves
the cleavage of the ester bond of one or more fatty acids in the
triglyceride structure, resulting in free fatty acids as they are
cut from the glycerol backbone. Chemicals, high heat (such
as deep frying), or lipase enzymes in the presence of water
can cause hydrolytic rancidity. The reaction can be either
desirable (as in the flavor development in mold‐ ripened
cheeses) or undesirable (rancid, “painty” or varnish‐like fla-
vor development in oils or fried products). The reaction can-
not be reversed; once the rancid flavor has developed, it is
permanent. And any foods prepared with rancid oil will taste
rancid as well; it will not be diminished by cooking.
Oxidative rancidity results most often from a complex
series of reactions between oxygen in the environment and
unsaturated fatty acids, causing the formation of a free radi-
cal due to the abstraction (removal) of a hydrogen atom from
the fatty acid molecule. The most reactive hydrogen atoms
(those requiring the least energy for their removal from the
fatty acid molecule) are attached to carbon atoms adjacent
to the C C double bonds. When the hydrogen is removed
from the carbon, the carbon is left with an unpaired electron,
and the resulting fatty acid radical is known as the alkyl radi-
cal. A series of structural shifts occur in the fatty acid once
the alkyl radical is formed; then, during the propagation
phase of lipid oxidation, the free radical covalently binds
with oxygen. The reaction terminates when this oxygen then
covalently binds to an additional hydrogen (which might
have been abstracted from another fatty acid, thereby cas-
cading and perpetuating the oxidation process), and the
structure becomes a hydroperoxide.
As the number of double bonds on a fatty acid molecule
increases (i.e., the molecule is more unsaturated), the suscep-
tibility of the fatty acid to oxidative reactions increases.
Polyunsaturated fatty acids (typically found in vegetable and
fish oils) are more likely to be involved in oxidative rancidity
reactions than monounsaturated or saturated fatty acids. This
is evident when comparing the oxidative rancidity reaction
rates in polyunsaturated fish oils, which develop off flavors
extremely rapidly, to more saturated butter or palm oil prod-
ucts. Increased oxidative rancidity occurs when the degree of
unsaturation in the fatty acids increases, oxygen or metal cata-
lysts (iron, copper, tin, nickel) are present, pro‐oxidants such as
lipoxygenase or singlet oxygen are present, and/or there is pro-
longed exposure to high temperatures or ionizing radiation.
Oxidative rancidity can be delayed by removing as much oxy-
gen as possible from the packaged product (using vacuum
packaging or gas flushing with an inert gas such as nitrogen, or
adding an oxygen‐scavenging sachet to the package), storing
products at low temperatures, and/or adding antioxidants
(ascorbic acid, tocopherols, BHA, BHT, TBHQ) or metal chelat-
ing agents (such as EDTA) to the product.
Proteins
Food proteins are composed of carbon, oxygen, hydrogen,
and nitrogen. Proteins, like carbohydrates, exist as small
building blocks that polymerize via unique covalent bonds to
form large molecules. The building blocks of proteins are 20
unique amino acids that contain amine (–NH
2
) and carboxylic
acid (–COOH) functional groups. These allow amino acids to
link together via strong covalent bonds known as peptide
bonds. Amino acids can only link together in a linear sequence.
Sequences of up to about 10 amino acids are known as pep-
tides, and sequences of many amino acids (typically from 100
to 500) are known as proteins.
The varieties of structure and texture that proteins give
to many foods surpass those of carbohydrates and lipids.
Proteins accomplish this diversity thanks to the different
polarities and unique side groups of the 20 standard amino
acids found in nature. Polarity is a measure of the orientation
of electronic charge in a molecule. Charged, partially
charged, or unbalanced molecules are polar and hydrophilic
(water‐loving) because water molecules themselves are
polar. Neutral molecules are non‐polar, unlike water, so they
are hydrophobic (water‐fearing). The polymer chain length
and sequence of amino acids determine the physico‐chemi-
cal, structural, biological, and functional properties of a
protein.
The 20 amino acids, with their unique polarities, form the
basis for a kind of code. In the same way that a binary
sequence of only 1’s and 0’s can represent any number, the
amino acid sequence of a protein can create countless varie-
ties of structures. A protein’s amino acid sequence is known
as its primary structure. The primary structure describes the
amino acid sequence but not the shape of the protein.
Protein shape and structure are so complex that proteins
are described by their secondary, tertiary, and quaternary
structures in addition to the amino acid sequence. The amino
acids of a protein chain attract and repel one another because
of their different polarities and their tendency to hydrogen
bond with each other. These interactions cause sections of
the protein to bend and fold into characteristic structures.
c02.indd 27 1/14/2016 7:11:30 AM
28 • CULINOLOGY®
Common structures include helices, sheets, turns, and ran-
dom coils. These structural configurations define a protein’s
secondary structure.
The tertiary structure of proteins is the three‐dimensional
organization of the complete (“folded” or “unfolded”) protein
chain as determined by the primary and secondary struc-
tures, the environment, and any processing treatments the
protein has undergone. Proteins in foods are generally divided
into two main classes of tertiary structures: fibrous proteins
(common in structural proteins, such as meat proteins) and
globular proteins (compact, spherical, or elliptical molecules,
such as enzymes, egg proteins, myoglobin, and the whey pro-
teins in milk).
In an aqueous (water‐based) environment, globular pro-
teins will fold into tertiary structures that minimize the expo-
sure of hydrophobic amino acids to the water, orienting these
amino acids in the interior of the folded structure. Heating a
globular protein above its denaturation temperature causes
an unfolding of the tertiary structure and exposure of these
hydrophobic amino acids to the environment. This unfolded,
denatured structure has different properties (including
reduced solubility and potential for precipitation, increased
viscosity, and gel formation) than the native, unheated pro-
tein. The shear forces created in mixing (such as whipping an
egg white) or alterations in pH can also denature the tertiary
structure of globular proteins. In general, globular proteins are
more sensitive to heat or shear than are fibrous proteins and
have lower denaturation temperatures.
Quaternary protein structures are created by the merging
of two or more proteins. Important examples of quaternary
structures are casein micelles in milk, which further aggregate
to form cheese; myoglobin, responsible for the color of meat
muscle tissues; and wheat gluten complexes (between glia-
din and glutenin wheat proteins). Wheat gluten is formed
from glutenin and gliadin when water and mechanical energy
(kneading) are added to make bread dough. Gluten com-
plexes form a viscous, elastic, and extensible mesh that traps
steam and carbon dioxide and allows breads to rise during
baking.
Physical Structures
(Amorphous and
Crystalline) of Solids
Foods are typically very complex mixtures of carbohydrates,
lipids, and proteins. On their own as pure ingredients, most of
these building blocks exist as solids at room temperature,
and often remain solid even during cooking. Oils are the only
significant exception. Because the solid state is the norm for
the majority of the building blocks of food, its properties
must be well understood.
Solid compounds can be classified into two groups of rel-
evance to foods: crystalline solids and amorphous solids.
Common crystalline ingredients include sugar and table salt.
To the naked eye, particles of granulated sugar and table salt
seem to have well‐defined geometric shapes. This is because
at the molecular level, each individual atom or molecule in a
crystal arranges itself in a consistent, repeating pattern. The
orderly geometry of a crystal observed by the naked eye
reflects the orderly geometry of its atoms or molecules at the
atomic scale.
(a) Primary structure
(b) Secondary structure
(c) Tertiary structure
(d) Quaternary structure
NH CH
Ri
NHCO CH
Ri+1
CO
Peptide bond
Amino acid residue
β-Sheet
α-Helix
Source: L. Mauer.
FIGURE 2.9 The primary, secondary, tertiary, and quaternary structure of proteins.
c02.indd 28 1/14/2016 7:11:31 AM
CHAPTER 2 • The Principles of Food Science • 29
Crystalline structure is not limited to individual crystal-
line ingredients. Many foods also contain crystalline solids.
Milk fat in cold butter exists as a crystalline solid, as do the
cocoa butter in chocolate and the amylopectin regions of
starch in native starch granules.
Common amorphous ingredients include powdered milk
and instant coffee granules. To the naked eye, these particles
seem to have no uniform shape of any kind. The same logic
applies to amorphous ingredients as to crystalline ones. Their
visible structures have no well‐defined geometric shapes
because their atoms or molecules arrange themselves in ran-
dom jumbles that lack a consistent, repeating pattern. The
random shape of an amorphous particle observed by the
naked eye reflects the disorderly geometry of its atoms or mol-
ecules at the atomic scale. Amorphous structures are typical
of most foods containing complex mixtures of ingredients.
The amorphous or crystalline structures of the assorted
solid components in a complex food dictate molecular mobil-
ity, water‐solid interactions, and the ultimate texture and
stability of the food. Food properties related to changes in
crystalline and amorphous structures include annealing
(heat treatment used in modification of starches), starch ret-
rogradation (a reaction in which the amylose and amylopec-
tin chains realign themselves into crystalline formations,
causing staling in baked goods and syneresis in starch‐
thickened puddings), film formation and properties, physical
aging, chemical and physical stability, encapsulation and
controlled release, stickiness, agglomeration (a process for
adhering smaller, finer particles together into larger clusters
or “agglomerates” used to enhance solubility of powdered
ingredients), and caking (unwanted clumping of free‐flowing
powders).
For all practical food purposes, solid fats (for example,
refrigerated butter), salt, and ice are crystalline structures.
Crystalline solids are characterized by having long‐range or
three‐dimensional atomic order and defined melting temper-
atures. Table salt (sodium chloride) is an ionically bonded crys-
talline structure; ice is a hydrogen‐bonded crystal; and the
relatively weak van der Waals forces between fatty acids in
lipids contribute to the crystalline structure of fats. Because
fatty acid and triglyceride structures vary in food fats, the
melting temperature range of fats is much broader than the
melting temperature range of the more uniform ice crystal
structure. A “plastic fat” (spreadable fat, such as room‐
tem perature butter) has both solid crystalline fat structures
and liquid oil regions in combination such that the plastic fat
behaves as a solid at rest but deforms (spreads) easily once a
force is applied.
Polymorphism describes a solid, such as cocoa butter in
chocolate, which can exist in more than one crystalline form.
Changes in the fat crystal structure can lead to unwanted fat
bloom and related texture and melting point changes in
chocolates, resulting in a product that does not melt in the
mouth but instead has a grainy or brittle mouthfeel. Crystal
structure changes during storage always shift to a more sta-
ble, lower‐energy polymorph. In the case of chocolates, how-
ever, the most desirable polymorph is not the most stable
form but can exist in a metastable state. The tempering of
chocolates is used to control the fat crystal structure by con-
trolled heating, cooling, and crystal seeding processes
designed to stabilize the desirable metastable crystal struc-
ture. However, improper tempering or storage in fluctuating
temperatures enables the metastable crystals to reorient
themselves into the most stable polymorph, which has a
larger crystal packing habit and higher melting temperature.
Because this melting temperature is higher than the temper-
ature in our mouths, the bloomed chocolate has a very differ-
ent texture than the desirable “melt‐in‐your‐mouth,” creamy
characteristics of well‐tempered chocolate. Control of tem-
perature, formulation, mixing, and storage conditions can
ensure desired crystal structures in foods (such as confec-
tioneries). Challenges can arise in foods with different lipid
systems in different components, such as a cookie with a
chocolate coating, wherein lipid migration or bloom can
result from interactions between the lipid systems, resulting
in unwanted sensory defects.
Water
Depending on your point of view, the preceding sections may
have been misleading: water is the true building block of food.
To the food scientist, water is H
2
O—a small, polar, ubiquitous
molecule that interacts with all foods and controls the way
foods change temperature (exchange heat with their environ-
ment), texture (lose crispiness or chewiness), shape (dissolve
in water), and even how likely they are to support the growth
of spoilage organisms or pathogens. The following sections
will explore water’s relationship to food in great detail
because of its central importance to all foods.
A water molecule is comprised of one oxygen atom and
two hydrogen atoms bound by strong O‐H covalent (“shared
electron”) bonds into an H‐O‐H structure. The electrons are
more attracted to the oxygen atom, which causes the oxygen
atom to have a slight negative charge and the hydrogen atoms
to have a slight positive charge, creating a large dipole
moment (separation of positive and negative charges) in the
water molecule. As a result, water is a polar molecule—that
is, one with a positive end (South pole) and a negative end
(North pole). This distribution of charges and polar traits
contributes to the ability of microwaves to heat foods. In a
microwave, the oscillating electric field causes the charged
water molecules within the food to spin, creating friction,
and this internal friction creates heat.
Water molecules interact with each other via hydrogen
bonds, attractive interactions between a hydrogen atom from
one water molecule and a lone pair of electrons on the oxygen
atom from another water molecule (the hydrogen‐bond
acceptor). As such, each water molecule can hydrogen‐bond
with up to four other water molecules via two hydrogen‐
bond donor and two hydrogen‐bond acceptor interactions.
The physical state of water (ice, water, steam) depends on the
extent of hydrogen bonding—ice has the most, steam has the
least. The network created between water molecules results
in large values for heat capacity, melting point, boiling point,
c02.indd 29 1/14/2016 7:11:31 AM
30 • CULINOLOGY®
and enthalpies (energy needed to break the hydrogen bonds)
of phase transitions between ice, water, and steam. Water has
a high surface tension, evident by the formation of water
droplets, due to the strong attraction of the water molecules
to each other.
Water is a common heat‐transfer medium used in tradi-
tional food preparation (boiling, steaming, simmering,
poaching, parboiling, blanching, stewing, braising, etc.).
There are numerous reasons why water must be controlled in
food preparation and processing: to prevent microbial growth
from causing spoilage or disease, to extend shelf life and
allow for distribution and storage by reducing the rates of
biochemical, chemical, physical, and microbiological reac-
tions; to minimize textural, quality, functional, and nutri-
tional changes during distribution; and to improve
convenience with new products. The amount of water in
foods can be controlled by dehydration or evaporation (using
dry-heat methods such as baking, deep frying, extrusion, air
tunnel, spray, drum, or freeze drying), crystallization of sol-
ids, the addition of humectants (compounds such as salts,
sugars, and glycols that promote the retention of water), or
freezing. While dehydration and evaporation both involve
physical removal of water, dehydration typically results in a
solid product such as dried fruits and vegetables, or powders,
whereas evaporation or concentration results in a viscous liq-
uid product such as sweetened condensed milk or juice
concentrates.
A food emulsion is a liquid system containing two phases
(continuous and dispersed) comprised of two immiscible
liquids (typically, oil and water) that is often stabilized by
emulsifiers. The creation and stabilization of emulsions is
important because water is polar and is a major component
of most foods, while lipids are non‐polar and are largely insol-
uble (immiscible) in water. Without an emulsifier present, the
density and solubility differences between the oil and the
water will promote separation, although increasing the vis-
cosity of the continuous phase (for example, by using gums or
starches to thicken water) or decreasing the droplet size of
the dispersed phase (for example, by homogenization of the
fat globules present in milk, or by high‐shear mixing in the
Ordered crystalline structure
Random amorphous structure
Source: M. Allan and L. Mauer.
FIGURE 2.10 Arrangement of atoms in crystalline and amorphous structures, and resulting appear-
ance under a polarized light microscope.
c02.indd 30 1/14/2016 7:11:48 AM
CHAPTER 2 • The Principles of Food Science • 31
preparation of “creamy” salad dressings or mayonnaise) can
slow the separation. Of course, in the case of mayonnaise, it is
not just high‐shear mixing that holds the emulsion together.
One of the key ingredients in mayonnaise is egg yolk, which is
rich in lecithin, a powerful emulsifier.
An emulsifier is an ingredient (sometimes called a sur-
factant, or surface active agent) that stabilizes an emulsion
by reducing interfacial tension (the tension that occurs in the
contact area between a hydrophilic and hydrophobic sub-
stance that creates a drive for separation) between the oil and
water. Different emulsifiers function in different ways: by cre-
ating repulsive forces between the dispersed droplets that
prevent them from coalescing; by creating steric hindrance, in
which the size of the emulsifier coating the dispersed drop-
lets prevents interactions between them; or by physically
separating the dispersed droplets from the continuous phase
via a solid adsorbed coating (such as paprika or mustard
solids in mayonnaise).
Some emulsifiers employ several of these mechanisms.
For example, amphiphilic proteins (compounds having both
H
Hydrogen bonds shown by dashed lines
H
H
H
HH
H
H
O
O
O
O
H
Negative charge
Positive charge
H
O
Source: L. Mauer.
FIGURE 2.11 Water: single molecule and multiple hydrogen‐bound molecules.
c02.indd 31 1/14/2016 7:11:48 AM
32 • CULINOLOGY®
hydrophilic and lipophilic groups) and phospholipids (like
the lecithin in egg yolks) orient themselves between the dis-
persed and continuous phases in an emulsion and provide a
coating that separates the phases and reduces interfacial
tension while also creating electrostatic repulsive forces to
keep the dispersed droplets apart. In theory, the selection of
an emulsifier is based on HLB values (the ratio between
hydrophilic and lipophilic groups in the emulsifier) and the
type of product the emulsifier will be added to. HLB values
for food emulsifiers range between 1 and 20; values of 1 to 8
indicate a more lipophilic emulsifier, and values of 9 to 20 cor-
respond to hydrophilic emulsifiers. Acetylated monoglycer-
ides have an HLB of 1.5, soy lecithin has an HLB near 8, and
polysorbates generally have HLB values greater than 10.
Emulsion stability is favored by the solubility of a given emul-
sifier in the continuous phase; therefore, lipophilic emulsifi-
ers work better in water‐in‐oil emulsions (such as oil‐based
salad dressings, sauces, and butters), while hydrophilic emul-
sifiers work better in oil‐in‐water emulsions (such as bever-
ages). In practice, most stable food emulsions contain more
than one type of emulsifier.
Water-Solid Interactions
Because combinations of solid food ingredients (fats,
carbohydrates, and proteins) and water are common in most
foods, understanding and controlling water‐solid interac-
tions is important for creating and stabilizing foods. Special
characteristics enable the water molecule to interact with
solids by various modes. These include its small size (which
makes it a good penetrant and space filler) and its ability to
form extensive hydrogen bond networks as a direct result of
its ability to act as both a hydrogen‐bond donor and acceptor.
Understanding how water interacts with solids is important
for ensuring shelf stability of food products.
Water interacts with solids through several mechanisms,
but deliquescence (adsorption and condensation of mois-
ture from the air leading to dissolution) by crystalline ingre-
dients, and absorption of moisture into the internal structure
of amorphous (non‐crystalline) materials are likely to have
the greatest impact on food product quality when solid
products are stored in environments containing high rela-
tive humidity.
Deliquescent, crystalline food ingredients such as sugars
(glucose, fructose, sucrose, lactose), inorganic salts (sodium
and potassium chlorides), organic acids (citric and acetic
acids), and vitamins (ascorbic acid, sodium ascorbate, some
of the B vitamins) are highly water soluble. Deliquescence is a
first‐order phase transformation in which certain crystalline
solids dissolve when the relative humidity (RH) of the sur-
roundings exceeds the critical relative humidity (RH
0
) of the
solid ingredient. A familiar example of deliquescence is the
caking of sodium chloride in saltshakers during the humid
summer months, when the RH exceeds 75 percent, which is
the RH
0
of NaCl. Deliquescence affects both the chemical and
physical stability of food products and is particularly impor-
tant in dry powder products, ingredient blends, and products
like candies and pralines in which crystallization is impor-
tant to control. Vitamin C is much more stable below its deli-
quescence point than above it. Individual ingredients are
Water
oil
Emulsifier
polar, hydrophilic region nonpolar, hydrophobic
region
Source: L. Mauer.
FIGURE 2.12 An oil‐in‐water emulsion depicting a continuous water phase, a dispersed oil phase, and
an amphiphilic emulsifier oriented between the two phases.
c02.indd 32 1/14/2016 7:11:48 AM
CHAPTER 2 • The Principles of Food Science • 33
more stable than ingredient blends in which interactions
between the ingredients lower the deliquescence RH and the
product becomes more susceptible to lower RHs. To avoid
deliquescence in ingredients, premixes, and products, it is
important to maintain storage conditions below the deli-
quescence RH of these products, both outside and inside the
package, by using packaging materials with appropriate bar-
rier properties or desiccants.
To promote the deliquescence of an ingredient or ingredi-
ent blend, the product can be stored at RHs above the deli-
quescence RH, or the deliquescence RH can be lowered to
below that of the surroundings. For example, sucrose can be
hydrolyzed by an invertase enzyme or acidic ingredient to
produce glucose and fructose, and the blend of ingredients
has a lower deliquescence point than the starting sugar. In
RH and temperature conditions in which the sucrose is stable
as a crystalline solid, the hydrolyzed blend of monosaccha-
rides deliquesces (dissolves) to form a sweet, viscous syrup.
An amorphous material is a non‐crystalline solid in which
the atoms have a less ordered arrangement than a crystal;
therefore, amorphous solids are not as compact in structure
as crystalline materials, and thus water can be absorbed into
their inner structure (much like a sponge absorbs water).
Absorbed water acts as a plasticizer and has numerous
effects on the structure and functionality of the material.
Some of these changes include decreases in mechanical
strength and viscosity and increases in chemical reactivity
and the tendency to crystallize. Amorphous solids can exist
in a brittle, glassy form or a viscous, rubbery form, depending
on the conditions (most notably temperature or RH). Think
of the difference in structure and texture of a fresh, crisp
cracker (glassy) and a stale cracker that has been exposed to
environmental RHs high enough to soften the texture (rub-
bery), or a soft candy at room temperature (rubbery) and that
same candy hardened at refrigerated temperature (glassy).
The temperature at which an amorphous solid transitions
between the glassy and the rubbery state is defined as the
glass transition temperature (Tg), the temperature at which
the amorphous solid becomes soft upon heating or brittle
upon cooling. Increasing the amount of absorbed moisture
decreases the temperature at which the glass transition
occurs because water has a very low Tg itself and acts as a
plasticizer. In temperatures at and above Tg or RH conditions
that lower the Tg below the environmental temperature, the
molecular mobility increases and the viscosity decreases,
resulting in structural changes such as loss of crispness, col-
lapse, and increased stickiness. Cotton candy is an example of
an amorphous product that is initially below its T
g
when
formed but becomes quite sticky after absorbing moisture
from a humid environment that drops the T
g
to below room
temperature, at which point the spun sugar threads collapse
into a sticky, viscous mass.
Understanding how water interacts with solids when they
are directly combined is important in preparing batters,
doughs, sauces, and beverages. To be functional in a product,
the ingredients must be soluble in water to some extent.
Solubility is the ability of a substance to dissolve; the sub-
stance that is dissolving is called a solute, and the substance
in which the solute is dissolved is called a solvent. A mixture
of solute and solvent is called a solution. A solid dissolves best
in a solvent with similar polarity: “like dissolves like.” Water is
polar and therefore readily dissolves polar solids; lipids are
non‐polar and do not dissolve in water. As a solid dissolves,
bonds that hold it together are broken, and molecules of the
solid are inserted into the structure of the solvent and sur-
rounded by its molecules. The hydrogen bonds in water read-
ily break and reform (which enables it to be poured) and will
readily form hydrogen bonds with polar solids (such as sug-
ars). Solids with charges (such as ionic charges in salts) also
readily dissolve in water, and salt forms of ingredients (such
as the sodium salt of ascorbic acid, known as sodium ascor-
bate, or sodium caseinate, compared to casein) tend to be
more soluble than the parent compound.
Beverages and sauces are obvious examples of foods
requiring high solubility of ingredients. If ingredients are
insoluble, they will precipitate out or float to the surface of
Water-Solid
Interactions
Surface
Interaction
Adsorption AbsorptionDeliquescence Crystal Hydrate
Condensed
Water
Internalized
Water
O
O
O
H
Food Surface
H H
H
H
H
Capillary
Condensation
Crystalline
Food
Crystalline
Food
Crystalline
Food
Condensed
Water
O
H H
O
H H
Source: M. Allan and L. Mauer.
FIGURE 2.13 Water‐solid interactions.
c02.indd 33 1/14/2016 7:12:01 AM
34 • CULINOLOGY®
the solution unless formulation strategies for stabilization
are employed (such as adding gums to chocolate milk to sus-
pend the insoluble chocolate solids, or adding an emulsifier
to keep the oil in a salad dressing in suspension). Proteins
may contain both hydrophilic and hydrophobic (polar and
non‐polar) amino acids and therefore have varying degrees of
solubility. Interestingly, the protein complex of wheat gluten,
once formed in dough, becomes insoluble in water. This
allows wheat bread doughs to expand and trap steam pock-
ets during baking, and durum wheat pastas to remain intact
during boiling. Wheat doughs can be washed in water, rinsing
away the starch fraction and leaving behind the water‐insolu-
ble mass of gluten. Although likely not appetizing, this gluten
mass can be baked to visualize the viscoelastic expansion of
the protein and entrapment of steam during baking.
The State of Water When
Solids Are Present
Water is a small and mobile molecule. In fact, water is con-
tinuously moving within food products, although the rate of
movement differs significantly depending on the location
and type of solids present, and the temperature. Water can be
classified into categories based on differences in mobility and
interactions with solids. In Zone I (low water activity and
moisture content), water is strongly attracted (sorbed) to the
surface of a solid. This is the least mobile form of water, and
the water acts as if it is part of the solid. Sometimes this water
is referred to as “tightly bound”; however, it is important to
keep in mind that water does not covalently bond to any-
thing and interacts only via weak hydrogen bonding net-
works that are constantly breaking and reforming. The term
“tightly bound” refers most closely to the difficulty in remov-
ing this type of water from a food system. It is nearly impos-
sible to remove all water from foods.
Additional water beyond Zone I can influence chemical
reactivity by acting as a reactant, product, medium, or a plas-
ticizer. In Zone II (intermediate moisture and water activity),
additional layers of water build up on the surface of the solid.
The attraction of this water to the solid is reduced compared
with Zone I water, but the water is still close enough to be
attracted to the solid surface. Due to this decreased (but still
present) attraction, Zone II water is more mobile than Zone
I water. This mobility enables reactions (such as Maillard
browning) that could not occur if only Zone I water was pres-
ent. As water activity and moisture content increase in
Zone II, water mobility increases and food texture softens.
Small increases in moisture content will lead to large
increases in water activity, which can have drastic effects on
the texture of dry or crispy food products (crackers, potato
chips, etc.).
When enough water is present to no longer be attracted
to the solid surface, the additional water is classified as bulk
phase or free water (Zone III). This water is highly mobile,
reactive (for example, in hydrolysis reactions), can act as a
solvent, and supports the growth and toxin production of
pathogens and the proliferation of spoilage microorganisms.
Many preservation processes attempt to eliminate spoilage
by lowering the availability of water to microorganisms.
Reducing the amount of free water also minimizes other
undesirable chemical changes that occur during storage.
The processes used to reduce the amount of free water in
consumer products include techniques such as evaporation
and concentration, dehydration, and freeze drying. Freezing
is another common approach to controlling spoilage. Water
in frozen foods is in the form of ice crystals and therefore
unavailable to microorganisms and for reactions with other
food components.
glassy, crispy state
rubbery, viscous state
Glass transition phase diagram for products
during storage
Moisture
T
e
m
p
e
ra
tu
re
glassy, crispy state
rubbery, viscous state
GainLoss
Moisture
GainLoss
In
c
re
a
s
e
D
e
c
re
a
s
e
T
e
m
p
e
ra
tu
re
G
lass transition
G
lass transition
In
c
re
a
s
e
D
e
c
re
a
s
e
Pasta dough
drying
Dry
pasta
b
o
ili
n
g
Ready-to-
eat pasta
Glass transition phase diagram for a pasta
product from production through preparation
Source: M. Allan and L. Mauer.
FIGURE 2.14 A glass transition phase diagram
for amorphous solids, showing the effects of
moisture content and temperature on the glass
transition temperature and state of the solid
during storage and during product preparation.
c02.indd 34 1/14/2016 7:12:02 AM
CHAPTER 2 • The Principles of Food Science • 35
Water Activity
Water activity (aw), is a measure of the relative escaping ten-
dency (also known as “fugacity” or tendency for evaporation)
of water from a food compared to the behavior of pure water.
Water activity has been loosely described as the availability of
water present in a food to act as a solvent and participate in
chemical or biochemical reactions, but it is important to note
here that the “a” in “a
w
” stands for activity, not availability or
amount. By definition, water activity is the ratio of the equilib-
rium partial vapor pressure of water in a system (p
w
) to the
equilibrium partial vapor pressure of pure liquid water (po
w
) at
the same temperature. The water activity can be measured as
the relative humidity (RH) directly above the sample at a con-
stant temperature (T) and pressure when in equilibrium.
Water activity is used as a measure of risk to understand
what microorganisms can grow in a food product (keeping in
mind that lower water activities do not necessarily kill the
microorganisms; they may remain dormant at a
w
s below
their threshold for growth) and what types of chemical reac-
tions might occur. The higher the water activity (up to a max-
imum of 1: the a
w
of pure water), the more likely that food will
spoil. In addition to influencing microbial spoilage, water
activity can play a significant role in determining the activity
of enzymes in foods and can have a major impact on their
color, texture, taste, and aroma.
As stated, the a
w
of pure water is 1, the a
w
of oil (or solids in
the complete absence of water) is 0, and most foods have an
intermediate a
w
between 0 and 1. As a rule of thumb, the
higher the a
w
, the more mobile the water and the faster it can
diffuse within a food or escape from it entirely.
It is important to note that the water activity of a food is
not the same thing as its moisture content. Water activity,
unlike moisture content, expresses how water behaves in
food instead of how much water is in the food. In a compari-
son of equal mixtures of sugar, salt, and sand (50 g) in water
(200 mL), the water activity of the sugar and salt solutions
was much lower than that of the sand mixture, even though
the water‐solid ratio was constant. Because there was little
interaction between the sand and water, the water activity of
this mixture (>0.998) was close to that of water (1). The
hydrogen bonding between the sugar and water slowed the
water’s escaping tendency, resulting in a water activity of
0.977. The a
w
of the salt solution was even lower, 0.851,
because salt dissociates into two ions (sodium and chloride)
in solution (compared to the single sugar molecule), and the
molecular weight of salt is less than that of sugar, resulting in
more molecules per gram (therefore more opportunities for
binding more water molecules), and ionic interactions of
water with salt are stronger than simple hydrogen bonding
with sugar. All of these factors decrease the ability of water to
escape from the salt solution, thereby lowering its a
w
.
When we try to expand the concept of a
w
and moisture
content relationships to food reformulations, such as reduc-
ing sodium or creating sugar‐free products, it becomes a
challenge to keep the water‐solid ratio constant. For example,
Source: L. Mauer.
FOOD
Water Activity
Zone IIZone I
0
M
o
is
tu
re
C
o
n
te
n
t
1
Zone III
O
HH O
HH
O
H
H
FOOD
FOOD
O
HH
O
HH O
HH
O
HH
O
O H
H H
H
O
H
H
O
H
H
O
H
H
O
HH
O
HH
O
HH
O
HH
O
HH
O
H
H
O
HH
O
H
H
O H
H
O
H
H
O
HH
O
HH
O
HH
O
HH
O H
H
O
H
H
O H
H
O
HH
O
HH
O
H
H
O
H
H
O H
H
O
H H
O
H
H
FIGURE 2.15 Type II moisture sorption profile typical of many amorphous foods, with “zones” of
water identified.
c02.indd 35 1/14/2016 7:12:02 AM
36 • CULINOLOGY®
a sugar‐sweetened strawberry jam has a lower a
w
than an
artificially sweetened one. In both jams, water binds to every
molecule of sweetener in order to dissolve it. But the sugar‐
sweetened jam contains far more molecules of sugar to bind
far more molecules of water than the artificially sweetened
one. This lowers the a
w
of the naturally sweetened jam to 0.87,
compared to the a
w
of the artificially sweetened jam, which is
closer to 0.95. When food products are reformulated to reduce
or remove sugar and salt, two ingredients that are capable of
lowering the a
w
of solutions, the reformulated product usually
has a higher a
w
.
Many foods have similar moisture contents but drastically
different a
w
s. Examples include honey and condensed milk,
with a
w
s of 0.56 and 0.84, respectively. Some foods with higher
moisture content have a lower a
w
than foods with lower mois-
ture content. Examples include sliced bread (with a
w
of 0.94)
and soy sauce (with a
w
of 0.86), even though the soy sauce has
more than twice the moisture content of the bread.
Moisture migration within a multi‐component food prod-
uct (for example, a filled pastry) or between a food product
and the environment is driven by differences in water activity,
not differences in moisture content. Water moves from a
region of high a
w
to a region of low a
w
until an equilibrium a
w
is reached. If the environmental RH is higher than the a
w
of
a food product (that is, high relative humidity in the air),
moisture will migrate into the product if a barrier package
does not separate the product from the environment. Potato
chips often lose their crispness after the package has been
opened because the environmental RH on a humid summer
day exceeds the a
w
of the product. In the case of a pastry with
a fruit filling, it is important to formulate the crust and the
filling to the same a
w
to avoid moisture migration from
the filling into the crust, making it soggy, or to provide an
edible moisture barrier between the crust and filling.
Adding deliquescent ingredients (soluble solids such as
salt or sugar) to food products can lower the water activity of
a product by interacting with the water and slowing down its
escaping tendency; however, the a
w
cannot easily be lowered
below the deliquescence RH of the added ingredient(s) with-
out removing water. Salt can be added to processed cheese
spread to lower the a
w
and enable a longer shelf life for
cheese‐and‐cracker snack products, although the deliques-
cence point of salt (75 percent RH) is higher than the a
w
of the
cracker (often near 0.3 a
w
, which is equivalent to 30 percent
RH), so an additional hurdle for moisture migration is
needed. Spray oil coating applied to crackers is one mecha-
nism to hinder moisture migration into the cracker.
Modifying the cheese filling formulations to blend a cheese
powder with lipids and flavors is another approach for lower-
ing the a
w
of the cheese filling to that of the cracker. Replacing
water with glycerol or oil can also lower the a
w
while main-
taining a soft texture in a product. Glycerol is commonly
added to raisins to maintain a soft texture in the presence of
crisp cereal flakes and is also added to tortillas to maintain a
soft texture for extended shelf life applications (including
space travel!).
Water activity affects chemical and physical degradation
reactions (for example, lipid oxidation, non‐enzymatic brown-
ing, loss of crispness) and microbial growth in food products.
In fact, it is a
w
, and not moisture content, that has been linked
to the growth of microorganisms, and there are upper and
lower critical a
w
s for a given reaction or microorganism. At an
White Bread
Wheat Bread
Soy Sauce
Reduced Salt Soy Sauce
Crispy Cookie
Chewy Cookies
Gummy Bears
Gummy Candy
Fat Free Yogurt
Yogurt
Sugar Free Hard Candy Beef Sticks
Mustard
Pudding
Sugar Free Pudding
Evaporated Milk
Fat Free Evaporated Milk
Condensed Milk
Fat Free Condensed Milk
Ketchup
Reduced Sugar Ketchup
Ketchup No Salt
Strawberry Preserves
Sugar Free Strawberry Preserves
Low Sugar Strawberry Preserves
Strawberries
Honey
Heavy Cream
Whole Milk
Skim Milk
Salted Butter
Unsalted Butter
Margarine
Cream Cheese
Fat Free Cream Cheese
Mild Cheddar
Extra Sharp Cheddar
Sour Cream
Light Sour Cream
Pepperoni
Mayonnaise
Light Mayo
Dill Pickles
Cucumber
Dry Noodles
Cotton Candy
Peanut Butter
Tomato Water
0%
10%
20%
30%
40%
50%
60%
70%
80%
90%
100%
M
o
is
tu
re
C
o
n
te
n
t
(w
b
)
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1
Water Activity
Source: Y. Ismail, Y. Shao, J. Sanchez, M. Allan, and L. Mauer.
FIGURE 2.16 The relationship between moisture content and water activity for a range of foods.
c02.indd 36 1/14/2016 7:12:02 AM
CHAPTER 2 • The Principles of Food Science • 37
a
w
of 0.85 and higher, pathogenic microorganisms can grow
and produce toxins in foods, while below a
w
0.85 the growth
and toxin production of pathogens is prevented. Therefore, a
potentially hazardous food (PHF) has an a
w
≥0.85 (and pH
greater than 4.6). Intermediate moisture foods (such as raisins,
jams, and jellies) have water activities in the range of 0.6 to
0.8, below the cutoff for pathogen growth. In this intermedi-
ate a
w
range, products can have soft, deformable textures, and
even though they won’t promote the growth of pathogens,
may still support the growth of molds. The critical a
w
lower
limit for any microbial growth is 0.6.
The Maillard reaction (non‐enzymatic browning) occurs
rapidly in this intermediate a
w
range; this reaction requires an
aqueous phase for the reactants (reducing sugars and pro-
teins) to interact; therefore, if the a
w
is too low, the reaction
will be inhibited due to the lack of molecular mobility, while
at an a
w
above 0.8, the reaction will be inhibited due to too
much water diluting the reactants. Enzyme activity increases
as a
w
rises above the minimum threshold because many
enzymes require mobility to interact with substrates (either
the enzyme or the substrate must have mobility).
Moisture Sorption Isotherms
Comparing water activity and moisture content enables the
product developer to determine which reactions might be of
concern for a given food product as well as the optimum
range for the desired texture and stability. Formulation strat-
egies, storage conditions, and packaging technologies can
then be developed to address the reactions that might limit
the quality of the product.
A moisture sorption isotherm (or water vapor sorption
isotherm) shows the relationship between the moisture con-
tent and water activity of a food (at a constant temperature
and pressure). The shape of an isotherm is governed by the
chemical components and physical structure of the food prod-
uct. When the moisture content is 0, the a
w
of the product is
also 0 (although this rarely, if ever, happens). As moisture con-
tent increases, a
w
increases, although the relationship is not
linear. Given enough time, food products equilibrate with the
humidity of the environment (an open package of crackers on
a humid day eventually results in a loss of texture when the
moisture content and a
w
exceed the crispy range). A moisture
sorption isotherm illustrates how the moisture content of a
food will respond to an increase or decrease in humidity
because the a
w
will equilibrate with the environmental RH.
Understanding these responses is important for the analysis
and design of various food transformation processes (e.g., dry-
ing, mixing, and storage), to predict changes in the chemical
and physical stability of foods, and for the selection of suitable
packaging materials. This in turn will influence the chemical
stability and physical properties of the product. Comparing the
moisture sorption isotherm of a product to the food stability
map helps the product developer determine what reactions
might be a concern in a food product with different moisture
contents and water activities.
Molecular Mobility
Another important contributor to food texture and stability
is molecular mobility (Mm)—a term used to describe the
movement and motion of food components. Chemical reac-
tions depend on the molecular reactants coming into con-
tact with each other with the proper orientation. To do this,
the molecules must be mobile, and the rate of the reactions
will depend on the amount of time it takes the molecules to
“meet” each other in the food. Small molecules (water,
oxygen) move more quickly than larger molecules (fats, pro-
teins, carbohydrates). Liquids have more mobility than sol-
ids, and gases are the most mobile. An amorphous solid in
0%
5%
10%
15%
20%
25%
0.00 0.20 0.40 0.60 0.80 1.00
%
M
o
is
tu
re
U
p
ta
k
e
Water Activity
Cotton Candy
Wh
eat
Cr
ack
er
FIGURE 2.18 The moisture sorption profile of
two different amorphous foods: cotton candy and
wheat cracker. Different formulations and food
types will exhibit different moisture sorption
profiles as environmental relative humidity
(equivalent to water activity) is increased.
Source: M. Allan and L. Mauer.
Moisture
Sorptio
n Isothe
rm
o
0 0.1 0.2 0.3 0.4 0.5
Browning
Reactions
Enzy
me A
ctivit
y
Mol
d G
row
th
Y
ea
st
B
a
c
te
ri
a
Water Activity
M
o
is
tu
re
C
o
n
te
n
t
R
e
la
ti
v
e
R
e
a
c
ti
o
n
R
a
te
0.6
L
ip
id
O
xid
a
tio
n
0.7 0.8 0.9 1.0
Moisture
Sorptioo
Loss of
crispness
Powder caking
Source: Decagon Devices, Inc.
FIGURE 2.17 Water activity. Stability of foods
as a function of water activity. Note the relative
reaction rates across different water activities. Also
note that foods formulated above water activity
0.85 must be temperature controlled or further
processed for safety.
c02.indd 37 1/14/2016 7:12:26 AM
38 • CULINOLOGY®
the glassy state (such as a crisp potato chip or spun sugar in
cotton candy) has less mobility than an amorphous solid
in the rubbery state (the same potato chip after exposure to
humidity loses its crispness, and cotton candy becomes a
sticky mass). The molecular mobility in crystalline solids is
the most restricted.
If molecular movement is slowed or inhibited, then the
stability of the food product will be enhanced because
molecular reactivity will be reduced. Water can act as a plas-
ticizer (a structure softener) and thus influence the molecu-
lar mobility of the material. This is seen in a cracker when it
loses crispness as humidity or moisture content increases,
the glass transition temperature is exceeded, and the struc-
ture enters the rubbery amorphous state. Mobility in disper-
sions depends on the state of the continuous phase. There is
more mobility in a viscous solution in which water is the
continuous phase than in a gel that has a protein or carbo-
hydrate as the continuous phase. A product containing a
filling (for example, fruit or cream) in contact with a pastry
crust maintains crust quality longer if the continuous phase
of the filling is in the form of a gel where water is the dis-
persed phase rather than a viscous solution where water is
the continuous phase. This is because the water is less
mobile when it is trapped in the dispersed phase rather than
free to move around as the continuous phase. Therefore, it
takes longer for the water to migrate from the filling into
the crust.
Heat-Driven Chemical
Reactions in Foods
Gelatinization of Starches
Native starch has both crystalline and amorphous regions,
which influences the important functionalities related to
granule swelling: gelatinization, pasting, and retrogradation.
Native undamaged starch is insoluble in cold water but
reversibly absorbs small amounts of water and swells slightly,
returning to its original size on drying. When starch is heated
in water, the starch granules begin to swell as the heat and
water disrupt the molecular order in the granules. As the
temperature rises, the molecular mobility in the granule
increases, more water enters the granule structure, and the
granule irreversibly swells. As temperature further increases,
hydrogen bonds are broken, and the crystalline regions in the
granule structure begin to melt and further disrupt the gran-
ule structure. This loss of molecular order is called starch
gelatinization. Amylose leaches out of gelatinized starch
granules into the surrounding aqueous environment and
thickens the solution, forming a starch paste or viscous solu-
tion with continued heating and stirring. During cooking, the
starch granules continue to rupture, leaching more amylose
and amylopectin into the solution and leaving starch granule
fragments or “ghosts.” For most food applications, starch
gelatinization is a prerequisite for obtaining the desired
functionality—that is, thickening, freeze‐thaw stabilization,
emulsification, and so on. Note: Pregelatinized or instantized
starches have had their molecular structure disrupted by pre‐
cooking to induce gelatinization and then have been dried
down for later use in instant products or foods where the
desirable functions of a cooked starch are wanted without
the need to cook the product.
Upon cooling of the cooked starch paste or solution, the
amylose and amylopectin that leached out of the starch
granules will begin to interact with other starch molecules,
forming small helical crystalline regions ( junction zones) and
possibly forming a gel. This process is called starch retrograda-
tion. Amylose tends to retrograde faster than amylopectin
(the branched structure of amylopectin slows down its retro-
gradation). Starch retrogradation is desirable for the forma-
tion of starch‐based gels; however, it can also lead to
undesirable quality changes such as syneresis (the contraction
of a gel accompanied by the separating out of liquid) in pud-
dings and custards, decreased viscosity and precipitation out
of solution in soups and sauces, and bread staling. Staling is a
slower proc ess that involves the retrogradation of amylopec-
tin; it can be reversed temporarily by reheating the product
above the melting temperature of the starch crystalline struc-
ture (as in toasting). To slow the staling process, additives
such as polar lipids and/or emulsifiers (e.g., lecithin, mono‐
and diglycerides) can be added to baked products; these inter-
act with the starch and delay retrogradation.
Denaturation of Proteins
Heat denaturation of proteins involves conformational
changes in the thermodynamically stable native structure of
the protein via unfolding or alteration of the quaternary, ter-
tiary, and/or secondary structure as a response to heat expo-
sure. Denaturation can disrupt hydrogen and disulfide bonds,
hydrophobic interactions, and salt bridges, but peptide bonds
remain intact. The primary structure, or amino acid sequence,
of the protein remains unchanged, as does the molecular
weight. Functional properties affected by denaturation include
changes in solubility, emulsifying capacity, gelation capacity,
foaming properties, and enzyme or biological activity. In addi-
tion to heat, denaturation can be caused by changes in pH, salt
25°C 55°C 65°C
gelatinization
Source: M. Allan and L. Mauer.
FIGURE 2.19 The effects of heat on wheat
starch in water, as observed under a polarized light
microscope. Gelatinization occurs when birefrin-
gence is lost.
c02.indd 38 1/14/2016 7:12:54 AM
CHAPTER 2 • The Principles of Food Science • 39
concentration, enzymes, addition of energy (shear, pressure),
or freezing. Some ingredients can delay denaturation or pro-
tect the protein, including sugars, some salts, and some
starches.
Gelation (Gel Formation)
Gelation occurs when a network of solids interacts in junc-
tion zones and entraps water (or other fluid). Although gels
are mostly liquid, they behave like solids due to their continu-
ous solid network. Many of the polymeric structures in foods
(proteins and polysaccharides) are able to form the continu-
ous networks characteristic of gels. A variety of junction zone
types are formed in the polymer matrices of different types
of polymers, including hydrophobic interactions, hydrogen
bonds, electrostatic interactions, covalent interactions, crys-
tallization, helix formation, and complexation. Stronger gels
are formed by more numerous junction zones, and junction
zones are formed by higher-energy interactions (such as
covalent interactions).
To create a protein‐based gel, the proteins dispersed in
water are often heated beyond their denaturation tempera-
ture, and the unfolded proteins interact or cross‐link to form
the continuous solid structure of the gel. The junction zones
formed between proteins and entrapment of water during
gelation may be either reversible or irreversible, depending
on the protein and type of junction zone. The polymeric net-
works formed by hydrogen bonding of gelatin molecules
(a fibrous protein) are thermo‐reversible. On cooling, the
gelatin molecules begin to interact in helical segments that
form junction zones leading to the formation of a gel; on
reheating, the hydrogen bonds break and gelatin returns to
the dispersed phase in the solution. Conversely, denatured
globular proteins often form thermo‐irreversible gels. When
exposed to heat, whey and egg white proteins first denature
and then interact via disulfide, hydrogen, and hydrophobic
interactions to form gels. These gels are heat‐set and stiffen,
instead of liquefy, when exposed to additional heat. The
term coagulation is often applied to irreversible heat‐setting
of proteins. Soy protein gels, such as tofu, are formed by elec-
trostatic bridges with divalent cations (calcium) between
the proteins. Treating proteins with the enzyme transglu-
taminase can result in stable covalently cross‐linked gels.
This is how the ingredient popularly known as “meat glue”
works.
Other polymers, such as starches and gums (for example,
agar and sodium alginate), can also form the continuous
phase in a gel structure. Starch gels are often formed by heat-
ing starch granules above the gelatinization temperature,
which enables the amylose to leach out of the granule struc-
ture, and then cooling down the paste. During the cooling
process, the amylose polymers realign themselves, form heli-
cal regions, and begin to crystallize in junction zones. When
enough junction zones are formed between the amylose mol-
ecules, the viscous solution or paste (in which water is the
continuous phase) converts to a gel structure in which the
amylose is the continuous phase. Many gums used in foods
are also able to form gels, and different gums form junction
zones by different mechanisms and bonds, resulting in a
variety of textures (tender, elastic, firm, brittle, malleable,
etc.) and appearances (clear, shiny, opaque, cloudy, etc.).
Unlike starch, some gums are able to form gels without heat-
ing, and a variety of cold gel structures are possible. Sodium
alginate, iota carrageenan, and low‐methoxyl pectins form
gels in the presence of calcium ions, known as ion‐coagulated
gels, wherein calcium ions form the junction zones between
these polymers. The alginate‐calcium gel system is used
extensively in the creation of an assortment of novel “caviars”
(usually fruit‐based) often seen where molecular gastronomy
is practiced. Synergistic interactions between locust bean
gum and xanthan gum lead to the formation of a firm
thermo‐reversible gel that is quite different from the hyper-
entangled solution structures of locust bean gum and xan-
than gum when in solution by themselves.
FIGURE 2.20 Properties of denatured proteins.
• Increased viscosity:
− Unfolded proteins
take up more space
Favorable in products like soups and gravies where consistency is important for consumer
acceptance.
• Aggregation to form
a gel
As in coagulated egg white or whey cheese due to denatured ovalbumin in egg white and
whey proteins of milk.
• Decreased solubility Affects the capacity of a protein to form emulsions, foams, or gels.
• Loss of biological activity
or altered reactivity
Favorable in the prevention of enzymatic browning by inactivation of enzymes such as
polyphenoloxidase, which cause browning in apples, avocados, and many other fruits and
vegetables.
• More digestible After they are unfolded, exposing more of their polymer structure to the environment, certain
proteins such as egg white become more susceptible to the action of proteases—enzymes
that break down proteins—thus improving the digestibility of the denatured protein versus
the raw one.
Source: L. Mauer.
c02.indd 39 1/14/2016 7:12:54 AM
40 • CULINOLOGY®
Non-Enzymatic Browning
Reactions
What makes many foods so appealing are the new flavors,
aromas, and colors produced during cooking with dry-heat
methods. Two main non‐enzymatic browning reactions, cara-
melization and the Maillard reaction, produce these benefi-
cial profiles. The result of these reactions is the reason bones
are roasted and vegetables are caramelized for use in stocks
and soups. Browned foods can have great‐tasting roasted fla-
vors, distinctive aromas, and rich dark amber colors.
Note: Certain foods, typically fresh fruits and vegetables,
can undergo enzymatic browning that is not dependent on
heating. Instead, these reactions typically take place at ambi-
ent temperatures, and are the result of cutting or bruising of
the fruit or vegetable whereby cells are torn open, releasing
enzymes (typically polyphenol oxidase), and the protective
peel is breached, allowing exposure to oxygen, which is essen-
tial for this enzyme’s activity. The enzyme acts on naturally
occurring phenolic compounds found in fruits and vegeta-
bles, converting them to melanins (brown pigments). This is
commonly seen in cut apples, bananas, avocados, potatoes,
mushrooms, and many others. This type of browning can be
inhibited by limiting exposure to oxygen (modified atmos-
phere or vacuum packaging), refrigeration, and acid dips to
reduce the pH below the range where the enzyme is most
active. Cooking will also eliminate this type of browning by
irreversibly denaturing the enzyme.
Caramelization
Caramelization is the non‐enzymatic browning of sugars when
they are heated for prolonged times in concentrated solutions
or dry forms, in the absence of proteins, particularly if the tem-
perature exceeds the melting temperature of the sugar. During
heating, sugar molecules expel water and undergo a series of
thermal decomposition reactions that result in the production
of brown‐colored compounds. The familiar result of carameli-
zation is the sweet brown flavor associated with caramelized
sugar made from the pyrolysis of sucrose, but not caramel can-
dies; these get their color from the Maillard reaction between
the milk proteins and sugars in the formula. Other sugars such
as maltose, fructose, and glucose also undergo pyrolysis,
though the caramelization point varies for each. These reac-
tions are mainly responsible for the rich colors and flavors that
develop in crème brûlée and in caramelized vegetables (onions,
mirepoix, roasted potatoes, etc.) through sautéing, roasting,
grilling, and frying—but not through any moist-heat cooking
techniques (the water prevents the temperature from rising
high enough). That is why recipes calling for “sweating” vege-
tables (cooking without any added liquid) require a lid to be
placed on the pan—to capture any moisture that exudes from
the vegetables in order to provide a humid environment to
prevent browning. Caramelization is also responsible for mak-
ing brittle‐type candies and caramel color, commonly used to
provide brown coloring to an assortment of foods and bever-
ages. Caramelization, if overdone, can also lead to undesirable
flavors and colors, including a burnt‐sugar smell.
Maillard Reaction
The Maillard (“my‐yard”) reaction, named after Louis‐Camille
Maillard, who investigated these reactions in the early 1800s, pro-
duces the roasted flavors and colors of products such as baked
goods, toasted bread, French fries, roasted coffee, malted barley
(for making beer and spirits), and seared meats. It is also respon-
sible for the off colors produced as a result of extended dry stor-
age of many foods, including powdered or condensed milk and
cereal. The reaction occurs between the carbonyl group of a reduc-
ing sugar and the amino group of a protein, and is another type of
non‐enzymatic browning. (Note: Sucrose is not a reducing sugar
and therefore cannot participate in Maillard browning without
first being hydrolyzed into its component glucose and fructose
monosaccharides.) The product then undergoes subsequent
rearrangements, fragmentations, and polymerizations to form
brown pigments called melanoidins. This reaction can happen at
room temperature after lengthy storage but is accelerated by high
heat, especially at intermediate water activities. The lowering of
the water activity on the surface of bread during baking facilitates
Maillard browning of the bread crust while the interior of the
bread remains unbrowned.
During the Maillard reaction it is possible to create hundreds
of new flavor components that would not have otherwise been
present in the food. The amino groups most commonly involved
in the Maillard reaction are those in the side chains of lysine and
histidine. Alpha amino groups of other free amino acids can
160°C
200°C
Crystalline sugars Amorphous
sucrose
G
a
la
c
to
s
e
M
a
lt
o
s
e
G
lu
c
o
s
e
F
ru
c
to
s
e
S
u
c
ro
s
e
FIGURE 2.21 Caramelization temperatures of
mono‐ and disaccharides. Response of crystalline
and amorphous sugars to heating. After 10 min-
utes at 320°F (160°C), crystalline sucrose and
galactose did not melt, crystalline maltose and
glucose melted but did not caramelize, crystalline
fructose melted and caramelized, and amorphous
sucrose caramelized. After 10 minutes at 392°F
(200°C), all sugars melted and caramelized. Brown
pigment differences are evident between the
different types of sugars as well as between the
crystalline and amorphous sucrose.
Source: Y. Ismail, Y. Shao, M. Allan, and L. Mauer.
c02.indd 40 1/14/2016 7:12:56 AM
CHAPTER 2 • The Principles of Food Science • 41
also participate. The type of amino acid reacting drives the
development of aroma more than the type of reducing sugar.
Each type of food, because of its unique combination of amino
acids, sugars, and the cooking technique used, can therefore
develop almost an infinite array of flavor possibilities. For exam-
ple, baked bread, grilled steak, and roasted peanuts all have
unique aromas and flavors that develop during heating.
That said, moist-heat cooking techniques (boiling, poach-
ing, steaming, etc.) do not support Maillard browning or the
flavors, aromas, and colors that are produced through these
reactions because the environment is too moist and the tem-
perature is not high enough. This is the main reason why
meats are seared before braising or stewing.
It is interesting to note that flavor chemists have taken
advantage of the Maillard reaction for many years to develop a
wide range of “process flavors” in the laboratory. These are often
characterized as roasted, toasted, sautéed, fried, or seared notes.
Heat as Individual
Processes
During cooking and many food processing operations, foods
are changed by added heat. The specific type of change
depends on the composition and thermophysical properties
of the food product, the heating temperature, and the heat
transfer medium (air, aqueous liquid, oil, etc.), as well as on
the rate and mechanism by which heat is transferred. To
understand heat transfer, the term heat must first be defined.
Heat is one of the ways in which systems, in this case food,
exchange energy with their surroundings. In many cases, the
exchange of heat between a food and its surroundings is easily
recognized due to the close relationship between heat and
temperature. However, there are instances in which the trans-
fer of heat does not produce a change in temperature but
rather a change in phase. Consider a container with liquid
water that is heated from a low temperature (77°F [25°C])
to the point where water begins to boil (212°F [100°C] at sea
level). During the heating process, energy is continuously
transferred from the heating source into the water. At the
beginning of the process, the temperature of the water
increases continuously. Heat transferred during this stage is
known as sensible heat. As more energy is transferred into the
system, the water molecules eventually contain enough energy
to overcome the attractive forces that keep them in a liquid
state and begin to vaporize. This is called the boiling point, and
at this point in the process, although energy continues to be
transferred into the water as long as the heat source is main-
tained, the water temperature remains constant at 212°F
(100°C), with more water molecules changing from the liquid
into the vapor state (steam). Heat transferred during this stage
is known as latent heat. In summary, the transfer of sensible
Fructose (closed form) Fructose (open form) Lysine
Glucosamine
polymerization
Hydroxymethylfurfural Melanoidins
OHHO
HO
O
O
O
H
HO
HO
HO
HO
OH
CH3
HO
HO
O
OH
OH O O
+
OH OH OH
OH
H2N
NH2
OH
R
N
H
HO
OH N
N
H
R
R
OH OH OH
HO
HOOH
OH
–H2O
OH
OH
N
N
R
n
R O
O
Source: J. Sanchez and L. Mauer.
FIGURE 2.22 Simplified Maillard reaction. This reaction mechanism follows the general sequence of
the Maillard reaction. The free amine on lysine attacks the open form of fructose to form a glucosamine;
the attack releases one molecule of water. Further dehydration can produce hydroxymethylfurfural, a
common Maillard product. Reactions with amino acids can generate nitrogen‐containing polymers known
as melanoidins, which contribute to the brown color formation.
c02.indd 41 1/14/2016 7:12:56 AM
42 • CULINOLOGY®
heat results in a change in temperature, whereas the transfer
of latent heat results in a change in phase (liquid water into
steam). A similar approach describes the freezing process,
where sensible heat is removed to the point where water
begins to freeze. Once the water molecules begin to crystallize,
the temperature remains constant during the phase change as
latent heat is removed from the crystallizing water.
The amount of energy required to change a given amount of
liquid water into vapor is different from the amount of energy
required to change that same amount of liquid water into ice.
Therefore, the type of phase transition must be specified within
the latent heat. Thus, water has a latent heat of vaporization
(liquid water to water vapor), a latent heat of fusion (ice to liq-
uid water), and a latent heat of sublimation (ice directly to
water vapor under vacuum conditions), depending on the spe-
cific change in phase. As seen in Figure 2.23, the pressure at
which a change in phase occurs is a function of the temperature.
Thus, the boiling point of water is lower or greater than 212°F
(100°C) for pressures below or above 1 atmosphere, respec-
tively. The pressure‐temperature dependence of the boiling
point can be observed by boiling water at different altitudes.
The higher you go, the lower the atmospheric pressure and the
lower the temperature required for water to boil. Therefore, it
takes longer to cook food if you live on a mountain; for exam-
ple, it takes longer to cook anything by boiling (eggs, pasta, rice,
vegetables) because the water boils at a lower temperature.
Recipes for certain bakery products must be adjusted for alti-
tude because decreased atmospheric pressure may result in
excessive rising, which stretches the cell structure of cakes,
making the texture coarse, or breaks the cells, causing the cake
to fall. This usually is corrected by decreasing the amount of
leavening agent. Increasing the baking temperature 15–25°F
(10–15°C) can also help “set” the batter before the cells formed
by the leavening gas expand too much.
Liquid
Gas
Solid
101.325 kPa
0.61173 kPa
22 060 kPa
1
32
4
Temperature
P
re
s
s
u
re
P
re
s
s
u
re
Temperature
Liquid
Gas
Solid
14.70 psi
0.089 psi
3 199 psi
1
32
4
1. Triple point
2. Normal freezing point
3. Normal boiling point
4. Critical point
1. Triple point
2. Normal freezing point
3. Normal boiling point
4. Critical point
0.01°C
32.02°F 212°F 705.47°F
100°C 374.15°C
Source: F. San Martin‐Gonzalez.
FIGURE 2.23 Phase diagram of water.
c02.indd 42 1/14/2016 7:12:56 AM
CHAPTER 2 • The Principles of Food Science • 43
Another example of the relevance of the pressure‐ tem-
perature dependence during cooking is in the use of pressure
cookers. A pressure cooker is a closed container in which
foods and an appropriate amount of water are exposed to
high pressures. Under pressure, the boiling point of water
rises, thus allowing foods to reach temperatures higher than
212°F (100°C) and requiring shorter cooking times. In indus-
trially processed (“retorted”) canned foods, these high pres-
sure–high temperature combinations are also used to
inactivate bacterial spores, which are resistant, latent forms
of certain microorganisms (in particular, Clostridium botu-
linum) that are not effectively destroyed by normal boiling
temperatures. Foods processed by these more severe heat
treatments are considered commercially sterile and can be
stored safely at normal room temperature for extended peri-
ods, provided that they are packed in a hermetically sealed
(airtight) container such as a can.
Phase Transitions
Phase transitions are useful in understanding why ingredients
behave the way they do. In pure systems, such as liquid water,
phase transitions are easily illustrated. The transition from liq-
uid to solid state results in ice, whereas liquid to vapor transi-
tion produces steam. While the chemical structure of the water
(H
2
O) remains the same through the phase transitions, its den-
sity, specific heat, and thermal conductivity are examples of prop-
erties that change with each phase. Changes in density explain
why ice, which has a lower density than liquid water, floats in
water. For pure substances such as water, phase transitions
(such as melting point or freezing point) can be predicted rela-
tively easily, while in the case of blended, multi‐ingredient food
systems, the presence of many different components results in
a more complex behavior. In more complex systems, phase
transitions tend to occur over a range of temperatures rather
than at a single point. Nevertheless, understanding the behavior
of each single component is a prerequisite to understanding
the behavior of more complex food systems. For example, lipids
in foods are called fats or oils depending on whether they exist
in solid or liquid state at room temperature. However, common
fats such as tallow, lard, or shortening are rather complex mix-
tures containing both liquid and solid phases of microscopic
triglyceride crystals. The proportion of solid triglycerides in any
given fat is known as its solid fat index (SFI), which, along with
the number, size, and kind of fat crystals, temperature, and
mechanical history (whether the fat has been agitated or mixed)
determines its consistency at any given temperature.
Thermal Properties
Thermal properties are important in cooking and processing
operations because they influence the rate at which heat is
transferred into or out of a food product. The thermal prop-
erty that specifies the amount of energy required to cause a
change in temperature of a given substance is called specific
heat (Cp). Think of specific heat as the amount of energy
required to increase the temperature of a given unit mass of
product by 1 degree. (Heat is usually expressed in calories,
kilocalories, or joules, and the degree is in Kelvin or Celsius.)
This property is important when designing food processes
and processing equipment to calculate the amount of energy
required to increase the temperature of a product from an ini-
tial temperature to a required final value. For example, the
specific heat of water is 4.18 joule/gram °C (that is, it takes
4.18 joules of energy to raise the temperature of 1 gram of
water 1°C), whereas the specific heat of butter is 2.04 joule/
gram °C. Therefore, if identical amounts of water and butter
were to be heated at the same starting temperature using
identical electric ranges, which one would you expect to
increase its temperature faster, water or butter? (Answer:
Butter heats faster because it has a lower specific heat.)
Another important thermal property that influences heat
transfer is thermal conductivity (k). Simply stated, this is a
measure of how easily a material conducts heat. In cooking,
the thermal properties of the cooking utensils are very
important. For example, copper has a very high thermal
conductivity —it heats up very quickly—and this property is
used to improve the heating efficiency and uniformity in pots
constructed with copper bases.
The thermal properties of pure substances are known. For
foods that contain considerable amounts of water (such as
stocks and broths), the values of thermal properties are simi-
lar to those of water. However, for more complex systems
(most other foods), the numerical values of thermal proper-
ties are a function of the composition of the product and the
temperature at which they are measured.
FIGURE 2.24 Thermal properties of liquid water, ice, butter, and air.
Property
Liquid water
@ 20oC
Ice
@ –18oC
Butter
@ 4.4ºC
Air
@ 20oC
Specific heat
(kJ/kg oC)
4.185 1.955 2.043 1.012
Thermal conductivity
(W/m oC)
0.602 2.370 0.197 0.0251
Density
(kg/m3)
997.0 919.4 — 1.164
Adapted from Singh and Heldman (2009) and Geankoplis (1993).
Source: F. San Martin-Gonzalez.
c02.indd 43 1/14/2016 7:12:56 AM
44 • CULINOLOGY®
In food processing and cooking, the thermal properties of
the heating medium are key factors in determining the rate of
heat transfer. The thermal properties of air, water, and oil for
operations involving baking, boiling, or frying are all different.
It is possible to heat fats and oils to very high temperatures
(350°F [177°C] to more than 400°F [204°C]), whereas water in
an open container can only reach its boiling temperature
(212°F [100°C]). On the other hand, air can be heated to high
temperatures, but it has a very low thermal conductivity;
therefore, air is not as efficient as fats, oils, or water in trans-
ferring heat. This explains why fried products cook faster
than boiled or baked products. This is also why you can place
your hand inside a 350°F (177°C) oven (at least for a short
time) but not in water boiling at “only” 212°F (100°C).
Heat Transfer
Heat transfer occurs when energy from a heat source is trans-
ferred directly or indirectly to the food. The heat source can
be a simple gas flame, an electric element, a microwave gen-
erator, an infrared appliance, or a magnetic element. Heat is
transferred to food in one of three basic ways: conduction,
convection, and radiation.
Conduction
Heat transfer by conduction refers to two things. First, it is the
mechanism by which solid food products are heated inter-
nally, with heat being transferred from hotter outer surfaces
to cooler interiors until the food is considered cooked and is
removed from the heat source. The rate at which a solid
product heats depends on the heat source and the product’s
thermal conductivity.
Second, conduction refers to a method of heating involving
direct heat transfer, where the food is heated due to direct
contact with a heat source. Conduction can happen between
any two solid objects as long as they touch, as in the transfer
of heat from a hot pan to a piece of food being sautéed. Heat
always flows from the hot areas to the cold areas, with the
rate of transfer being faster or slower depending on the size of
contacted surface area and the difference in temperature
between the hotter and colder surfaces.
Convection
Convection is the transfer of heat through moving fluids, such
as air, water, or oil. Heat transfer by convection is the result of
molecules physically moving from one location to another
and exchanging energy with molecules at the other location.
It occurs at the interface between heated liquids or gases and
solids. The physical movement of molecules is caused by
changes in density and viscosity within the heating medium
due to differences in temperature. If the heated molecules
move freely as a consequence of gravity, the proc ess is known
as natural convection. Due to the natural tendency of hot liq-
uids and gases to rise and colder ones to fall, natural convec-
tion occurs in any oven or fryer. Inside a fryer, the oil that has
been cooled by the submersion of colder food naturally falls
and the hot oil rises, creating a current of oil inside the fryer.
On the other hand, if the fluid is moved by mechanical action,
Fire
(Radiation)
Air
(Convection)
Air
(Convection)
Turkey
(Conduction)
Pan
(Conduction)
Source: F. San Martin-Gonzalez.
FIGURE 2.25 Modes of heat transfer: conduction, convection, radiation. Inside an oven, the fire emits
radiant heat, which in turns heats the air in the oven. The air transfers heat by convection to the surface
of the turkey, the pan, and the walls of the oven. When the heat reaches the surface of solids (the turkey,
the pan, the walls), it is transferred by conduction. For all practical purposes, solid foods are considered
to behave as solids.
c02.indd 44 1/14/2016 7:12:57 AM
CHAPTER 2 • The Principles of Food Science • 45
such as fans or agitators, the process is known as forced con-
vection. In a convection oven, the movement of the hot air
from hotter to colder areas is assisted by the addition of a
mechanical blower or fan inside the oven. It must be noted,
however, that heat transfer by convection occurs only at the
food’s surface. Once the energy is transferred from the heated
liquid or gas to the surface of the solid food, the food keeps
transferring the heat to its interior by conduction.
Thermal Radiation
Heating by radiation (or “radiant energy”) is the result of elec-
tromagnetic radiation (in the form of light photons) being
absorbed by a given food’s surface. (Note: This is a form of
non‐ionizing radiation. It has nothing to do with radiation
caused by nuclear reactions or radioactive materials, which is
ionizing radiation.) No medium (hot air, hot oil, a hot pan) is
needed between the food and the radiant heat source for heat
transfer to take place (unlike with conduction and convec-
tion). Like convection, heat transfer by radiation is also a sur-
face phenomenon. One example of heating by radiation is the
use of infrared heat lamps to keep foods hot. Another is broil-
ing, where the heat source is hot enough and close enough to
cook the food without direct contact. Recently a radiant fryer
was launched, where partially fried products are exposed to
radiant energy, thereby increasing the temperature on the
surface of the food and developing the appropriate texture
without using large volumes of oil.
Microwave Heating
Microwave heating deserves special consideration due to
the extensive use of microwave ovens in many households. It
is also used in commercial food manufacturing as an aid to
thawing and in heat processing. Microwave energy is a form
of electromagnetic radiation that uses microwave radiation
rather than light as the generator of heat. In addition, unlike
other forms of heating, which are primarily surface heat
transfer phenomena, microwave heating takes place at the
molecular level of the food product. When a food product is
exposed to microwave energy, heating occurs internally by
the friction created by water molecules (and other charged
atoms or molecules) trying to align themselves under the
rapidly oscillating energy field. This results in foods heating
volumetrically—that is, the food heats simultaneously at
various depths, as opposed to other forms of heating, where
the surface is heated first and then the heat is conducted to
the interior of the solid. The volumetric heating effect is a
consequence of the ability of microwaves to penetrate the
food below the surface level. Penetration depth of micro-
waves is dependent on food composition and the frequency
of the microwave generator, with lower microwave frequen-
cies (longer wavelengths) penetrating further. That said,
maximum penetration depth is only a few centimeters;
microwave ovens do not cook foods “from the inside out.”
Induction Heating
Induction heating is a method of instantaneously raising the
temperature of a pan made of ferromagnetic material by
applying a magnetic field generated by a powerful, high‐
frequency electromagnet. This in turn heats whatever is
inside the pan by conventional conduction or convection
mechanisms. Unlike other forms of cooking, heat is gener-
ated directly in the pan rather than generated in the stovetop
by electrical coils or burning gas. The amount of heat gener-
ated can be moderated by controlling the strength of the
electromagnetic field ( just like raising or lowering the gas
flame or electric coil on a standard stove). The material of
which induction cookware is made is very important for
induction heating to work: it must be magnetic. Cast iron and
stainless steel are suitable, whereas aluminum (unless it has a
steel bottom), copper, and Pyrex are not. An induction cooker
is faster and more energy efficient than a traditional gas or
electric stove. It allows rapid control of cooking energy simi-
lar to gas burners. Because induction heats the cooking ves-
sel itself, the possibility of a burn injury is significantly less
than with other methods; the surface of the cooktop is heated
only from contact with the pot or pan. There are no flames or
red‐hot electric heating elements, as in traditional cooking
equipment. Also, because energy is not wasted heating the
air around the cooking vessel, induction cooking is much
more energy efficient than gas or electric.
c02.indd 45 1/14/2016 7:12:57 AM
46 • CULINOLOGY®
3
Lead Authors: Charlie Baggs, President and Executive Chef , Charlie Baggs, Inc.
Herbert A. Stockschlaeder II, CRC®, DTR, Director, Technical Services, Rosina Food Products, Inc.
Review of Cooking
Techniques
Understanding
Cooking Techniques
from a Chef ’ s Viewpoint
Cooking has long been used as a means to alter raw food
materials into a more palatable and nutritious form, render
them safer for eating and storing, and provide a broader
range of flavors than their raw form. Over centuries of experi-
mentation and documentation, chefs have created a univer-
sal set of cooking methods that are the foundation for all
modern cooking techniques. Heat and other forms of energy
applied to food alters its structure, nutrition, sensory attrib-
utes, and chemical makeup in a wide variety of ways.
Modern cooking techniques typically involve some or all
of the heat transfer methods mentioned in the preceding
chapter, and they can all be classified into two main catego-
ries: dry‐heat and moist‐heat cooking.
Dry‐heat cooking methods do not involve the use of water
in the cooking process. These methods include baking, roast-
ing, grilling, broiling, barbecue, sautéing, stir‐frying, pan‐fry-
ing, and deep frying. Foods are cooked with heat transferred
directly through exposure to an open flame, hot air, hot sur-
face, radiant heat, or hot fat or oil.
Moist‐heat cooking methods use water or steam to trans-
mit heat to foods. These include blanching, simmering, boil-
ing, steaming, poaching, stewing, and braising. Braising and
stewing also usually incorporate an initial searing step (a dry‐
heat method) for flavor and color development prior to com-
pletion of the cooking in a moist‐heat environment. As a
result, these are sometimes referred to as combination cook-
ing methods .
Dry‐ and moist‐heat cooking are drastically different in
the ways they act on the food to produce a broad and varied
array of tastes, textures, aromas, and appearance. These two
types of cooking methods also have greatly different effects
on the development, concentration, and evaporation of fla-
vor components.
Dry‐Heat Cooking
Methods
During dry‐heat cooking, the outer surface of a food is sub-
jected to elevated temperatures in a water‐free environment.
This develops flavors and browning by a combination of car-
amelization and the Maillard reaction. It is commonly used
as a preliminary step to develop color and flavor in meats and
vegetables before finishing cooking using other methods.
Contrary to popular belief, however, searing does not “seal in”
juices in preparation for follow‐up cooking. It has been shown
scientifically that meat loses the same amount of moisture
whether or not it is seared before final cooking.
Baking
Baking uses heat transmitted by the convection and conduc-
tion of hot air to cook foods inside an oven or other heatproof
cooker. Any source of heat—gas, charcoal, wood, or electric
element—can be used. Heat transmission can occur via con-
duction alone or be enhanced by the use of a convection oven
(via air flow created by a fan inside the oven). The forced hot
air provides a more even temperature throughout the oven
and speeds the transfer of heat, allowing for lower cooking
temperatures, shorter cooking times, and less expenditure of
energy. Baking is typically used for “baked goods,” that is,
breads and pastries, pies and cakes, cookies and crackers. But
many other foods are also baked: ham and meatloaf, cas-
seroles and quiches, flans and soufflés, pizza and lasagna,
potatoes, apples, baked beans, and winter squash. There is a
fine line between baking and roasting, which are virtually the
c03.indd 46 1/22/2016 12:57:16 PM
CHAPTER 3 • Review of Cooking Techniques • 47
same cooking process. Whether a food is said to be baked or
roasted is pretty much the result of historical terminology
rather than clear-cut identifiable differences in the cooking
process. Regardless of what you call it, dry‐heat cooking in
heated air causes a change to the structure of sugars, resulting
in caramelization, and an interaction of proteins and reducing
sugars through the Maillard browning reaction. Both of these
contribute to the development of colors, aromas, and flavors
made possible only with dry‐heat cooking methods.
Roasting
Like baking, roasting uses hot air as the heating medium in
an oven or above an open flame, as in rotisserie cooking,
wood‐fired oven cooking, and “spit‐roasting.” Heat is trans-
ferred by convection from the air to the surface of the food
and by conduction from the surface of the food to its interior.
The heating medium is dry, and the process is designed to
prevent the foods from simmering in their own juices as they
are exuded, either by suspending the food above the flame or
by placing it on a raised rack inside the roasting pan.
Roasting is usually used to cook large, tender cuts of meat,
but it can also be used for coffee beans, vegetables, or pea-
nuts. Vegetables such as potatoes, shallots, cauliflower,
squash, onions, and others with high sugar concentrations
are very flavorful when prepared this way because of the car-
amelization of the sugars. A perfectly roasted item should be
a rich amber or golden brown color and have an evenly
crisped surface.
Roasting meat should be placed fat side up; in this manner
it bastes itself. Added fat, meat juices expelled during roast-
ing, or sauces can be used to baste meats during cooking to
provide additional flavor. The goal is an evenly crisp and well‐
browned surface.
Interior portions of roasting foods cook slowly by conduc-
tion alone. The larger the piece of meat, the longer it takes to
roast because of the greater distance the heat must travel to
reach the center. When cooking a large piece of meat, it is a
good idea to start with an oven temperature of 450 to 500°F
(204 to 260°C) to quickly brown the outside of the meat. Once
good color is achieved, the oven temperature is reduced to 225
to 350°F (107 to 177°C) for the rest of the cooking period. The
objective is to cook to the proper temperature throughout the
meat without overcooking the exterior. Shielding the food’s
surface with aluminum foil can slow the browning process
while the interior continues to cook. It is important to balance
browning the outside of the product evenly with cooking the
center of the product to the exact desired doneness.
Keep in mind that for nearly every cooking method, the
larger the food, the longer the residual or carryover cooking
that will occur. Cooking doesn’t stop immediately when the
heat is turned off. When food is removed from the heat
source, initially the outside of the food is much hotter than
the center. However, heat continues to move into the food’s
interior (by conduction) until the entire food reaches equilib-
rium. Therefore, it is important that larger foods be removed
from the heat when the core temperature is lower than the
final desired temperature, as it can rise as much as 10 to 20°F
(5 to 10°C) before the product begins to cool.
Establishments producing cooked meat and poultry
products are required by the Food Safety and Inspection
Service (FSIS) of the United States Department of Agriculture
(USDA) to meet specific lethality standards for the reduction
of salmonella. To assist establishments in meeting the lethality
requirements, FSIS has issued compliance guidelines, which
are based on established time/temperature thermal process-
ing requirements to achieve the required level of lethality.
Figure 3.2 Pistachio‐crusted roasted meat.
FIGURE 3.3 Internal beef doneness
temperatures.
130°F = rare
140°F = medium rare
150°F = medium
155°F = medium well
160°F+ = well
Source: Charlie Baggs, IncSource: Charlie Baggs, Inc.
FIGURE 3.1 Baked goods.
Source: Charlie Baggs, Inc.
c03.indd 47 1/14/2016 7:14:23 AM
48 • CULINOLOGY®
These guidelines (in table form) provide the acceptable
amount of time that various types of meat and poultry prod-
ucts must be held at different internal temperatures in order
to achieve a 6.5‐log or 7‐log reduction in living Salmonella
organisms. Log reduction is a mathematical term used to
show the reduction in the number of live germs logarithmi-
cally. It denotes the relative number of live microbes elimi-
nated from a food due to thermal processing (or other
microbial control treatments). A 1‐log reduction means the
number of germs is 10 times smaller than before heating.
A 2‐log reduction means the number is 100 times smaller, a
3‐log reduction is 1,000 times smaller and so on up to 7‐log
reduction (10,000,000 times smaller). FSIS requires a 7‐log
reduction for poultry and 6.5‐log reduction for red meat. A
7‐log reduction is included in the red meat chart for those
processors wanting an extra safety net.
In addition to time/temperature heating tables, the FSIS
also provides cooling tables. Both sets of tables can be found at:
Compliance Guidelines for Meeting Lethality Performance
Standards for Certain Meat and Poultry Products (http://www
.fsis.usda .gov/OPPDE/rdad/FRPubs/95‐033F/95‐033F_
Appendix%20A.htm).
Safe Minimum Cooking Temperatures
Use the chart in Figure 3.4 and a food thermometer to ensure
that meat, poultry, seafood, and other cooked foods reach a
safe minimum internal temperature. Remember, you can’t
tell whether meat is safely cooked by looking at it. Any
cooked, uncured red meats, including pork, can be pink even
when the meat has reached a safe internal temperature.
Why Resting Time After Cooking
Is Important
After removing meat from a grill, oven, or other heat source,
allow it to rest for the specified amount of time (usually
10–20 minutes depending on the size of the roast). During
the rest time, its internal temperature will continue to rise
until the entire piece of meat reaches equilibrium. This
allows internal juices, which have been pushed toward the
surface as the interior temperature rises, to redistribute
throughout the muscle, reducing drip loss when the meat is
cut or sliced.
Grilling
Grilling is one of the oldest and most dynamic cooking meth-
ods. Grilling often implies the use of hardwood or coals, which
impart a distinctive taste and additional complexity from the
smoke in addition to the “char” flavors that develop from the
intense heat. Charcoal provides a strong heat source with
minimal flavor impact of its own. The smoke coming off the
grill is normally the fat melting and burning off the hot embers.
Grilled flavors are usually much more pronounced than those
created during roasting. When grilling, it is especially critical
to control the amount of browning, as charred flavors have a
bitter component that must be carefully balanced. That said,
Category Food
Temperature
(°F) Rest Time
Ground
Meat & Meat
Mixtures
Beef, pork,
veal, lamb
160 None
Turkey,
chicken
165 None
Fresh Beef,
Veal, Lamb
Steaks, roasts,
chops
145 3 minutes
Poultry Chicken &
turkey, whole
165 None
Poultry
breasts, roasts
165 None
Poultry thighs,
legs, wings
165 None
Duck & goose 165 None
Stuffing
(cooked alone
or in bird)
165 None
Pork and
Ham
Fresh pork 145 3 minutes
Fresh ham (raw) 145 3 minutes
Precooked
ham (to reheat)
140 None
Eggs & Egg
Dishes
Eggs Cook until
yolk and white
are firm
None
Egg dishes 160 None
Leftovers &
Casseroles
Leftovers 165 None
Casseroles 165 None
Seafood Fin Fish 145 or cook
until flesh is
opaque and
separates easily
with a fork.
None
Shrimp,
lobster,
and crabs
Cook until
flesh is pearly
and opaque.
None
Clams, oysters,
and mussels
Cook until
shells open
during cooking.
None
Scallops Cook until flesh
is milky white
or opaque and
firm.
FIGURE 3.4 Safe minimum cooking
temperatures.
Source: Foodsafety.gov.
c03.indd 48 1/14/2016 7:14:23 AM
CHAPTER 3 • Review of Cooking Techniques • 49
the charred flavor provides a mouthwatering savory building
block that can be used to create wonderful, deep flavor
experiences.
Grilling is not to be confused with barbecuing (cooking
“low and slow” in an enclosed heated box). Grilling is more
like broiling—cooking one side at a time, in this case from the
bottom up, with intense radiant heat. In commercial manu-
facturing, the traditional use of wood or charcoal has largely
been replaced with gas‐fired burners. While this has elimi-
nated the wait for wood or charcoal to generate the right heat
level and provides a greater level of control, efficiency, and
consistency to the process, there is less depth of flavor devel-
opment due to the absence of the complexities that wood
smoke imparts. Using smoke, roast, and grill flavors can com-
pensate for this to some degree.
Items that are grilled are usually seasoned via marinating or
dry rubs before they are cooked. Meats can also be seasoned
while on the grill. Remember, though, if the food item is to be
exposed to high temperatures for extended periods, high‐sugar
sauces (barbecue sauces or glazes) and seasonings should be
applied late in the cooking process so as not to burn.
Typically, tender, thinner cuts of meat and vegetables are
grilled or broiled because they do not need extended periods
of moist cooking to break down connective tissues. They
must be turned partway through the cooking process.
Because fruits and vegetables have high concentrations of
sugar, they tend to caramelize (and scorch) faster than
meats, so care should be taken not to burn them. The best
way to cook on the grill is to set up high and low heat “zones.”
Caramelize the exterior of the food quickly over the high
heat zone, then move the food to a cooler area of the grill to
allow the heat to penetrate the product without further
charring.
Broiling
Broiling is closely related to grilling, but the radiant heat
source is above the food, and wood is never used as a fuel.
Modern broilers are designed to use both convection and
radiant heat, and are typically either gas‐fired or electric.
Often, delicate items such as fish are broiled on an oiled
metal heat‐safe platter (sizzle platter) that can withstand the
high temperatures in a commercial broiler (700+°F [370+°C]).
This eliminates the potential for the fish to stick to the broiler
grate. Broiling is also used to “finish” a product by quickly
adding a golden brown surface or melting a topping, or doing
both, such as browning cheese on French onion soup or the
sugar on a crème brûlée. These products can be browned
quickly on the surface while the interior temperature of the
rest of the food remains largely unaffected. In the restaurant
kitchen, the salamander broiler is specifically designed for
this purpose.
In commercial settings, broiling temperatures can reach
up to 1000°F (538°C), depending on the type of heat source
used. This high heat facilitates the Maillard browning and
caramelization reactions on the outside of the food by imme-
diately lowering the surface water concentration and cook-
ing the outside by direct radiant heat. Depending on the
thickness and delicacy of the product, the radiant heat may
be enough to completely cook it. If it is not, the interiors of
thicker products cook by conduction. To perfectly cook a
thicker piece of food, it must be located in exactly the proper
location relative to the heat source so the outside doesn’t
overcook while the inside remains underdone. One benefit of
using a top‐mounted broiler instead of a grill is that juices
dripping from the cooking food do not drop onto the heat
source (hot coals, gas burners, etc.), causing smoke and
undesirable flavors to rise back onto the food.
Barbecue
Unlike grilling, which is hot and fast, barbecue is a process of
cooking meats in an enclosed chamber at a relatively low
temperature (usually 225 to 350°F [107 to 177°C]) for an
extended period, usually in conjunction with smoking. In
fact, because barbecue is carried out in an enclosed chamber
where the humidity is higher than in typical dry‐heat meth-
ods, it can be considered a combination cooking method
(moist and dry heat together). Popularized in the southern
United States, “low and slow” is barbecue’s motto. Larger cuts
Source: Charlie Baggs, Inc.
FIGURE 3.5 Grilled chicken with mushroom sauce.
Source: Charlie Baggs, Inc.
FIGURE 3.6 Broiled pork chop.
c03.indd 49 1/14/2016 7:14:46 AM
50 • CULINOLOGY®
of meat from the shoulder or leg that have plenty of fat mar-
bling and lots of collagenous connective tissue are perfect for
this method.
Collagen is the main component of connective tissue and
the most abundant protein in mammals, making up 25 to
35 percent of the whole‐body protein content. Collagen is
mostly found in fibrous tissues such as tendon, ligament, and
skin, and is responsible for the tough and chewy cuts of meat
found primarily in the leg and shoulder muscles of meat ani-
mals. When exposed to heat and moisture, the collagen
hydrolyzes into gelatin, a protein with “melt‐in‐the‐mouth”
texture and exceptional flavor release.
The lower temperature, longer cooking time, and higher
humidity in the enclosed barbecue cook chamber break
down the fat and collagen, while the smoke adds excep-
tional flavor. The meats are seasoned, placed indirectly
above or next to the heat source, and allowed to cook slowly
for hours until the fat and connective tissues melt and cre-
ate a moist, tender, succulent product. When properly pre-
pared, slow‐cooked barbecue falls off the bone and melts in
the mouth.
Beef, pork, whole turkeys, chicken, lamb, bison, duck, and
mutton can all be cooked successfully using this method.
The characteristic flavor of barbecued meats is often due
to the creative use of wood types. Hardwoods such as hickory,
oak, apple, and mesquite, as well as wood from other fruit
and nut trees, make excellent additions to the barbecue fire.
Saplings and soft woods are not generally used because their
resins and sap contain phenols and other undesirable compo-
nents, which create acrid, bitter flavors and may form unsafe
chemical compounds on the food. Successful smoking is evi-
denced by the presence of a pink “smoke ring” that can be
seen near the surface of meat when it is sliced; the wider the
ring, the more smoke the meat has absorbed. This happens
when nitrogen dioxide from the burning wood combines
with myoglobin in the muscle tissues to form nitrites and
nitrates. These cure the meat, similarly to the way hams and
bacon are cured, and also the way they get their pink
coloration.
Hot‐Oil Cooking
Methods
Considered a type of dry‐heat cooking because it does not
rely on moisture for heat transfer (in fact, this technique
removes moisture from food during cooking), frying, when
properly executed, is an excellent way to keep foods succu-
lent and evenly browned on the outside. The foods that ben-
efit most are small or thin cuts of tender meats or vegetables
(although in recent years, even full‐size turkeys are being
deep fried). Potatoes, doughnuts, and many other carbohy-
drate‐based products are also fried. The sugars and small
amounts of proteins within these foods react to form Maillard
browning compounds, resulting in tasty, golden‐crisp coat-
ings and flavors. Meat tenderness can be affected because
frying is a high‐temperature, rapid method of cooking, so
muscle fibers shorten more severely than with slower meth-
ods of cooking. Therefore, only thin or tender cuts with little
to no connective tissue should be fried. Fish, shrimp, scallops,
chicken, pork, and tender cuts of beef are the best choices.
Sautéing
Sautéing (from the French for “to jump”) is rapidly cooking
foods in small amounts of oil over high heat. This method is
used for quickly searing and browning foods with a limited
amount of hot oil. Before sautéing, meats are usually lightly
dusted with flour to enhance browning and reduce sticking
to the pan, shaken to remove excess flour, and then placed
presentation side down into the sauteuse—a wide, shallow
pan that is curved outward where the sides meet the bottom.
This aids in flipping or making the foods “jump” without the
need for utensils.
Sautéing is the cooking method of choice for “à la minute”
(“in a minute”) cooking. Because the foods cooked in this
manner are usually small and tender, they cook very quickly.
When a larger food, such as a whole chicken breast, is sau-
téed, a two‐stage approach is used. First, the presentation
side (skin side) of the breast is seared on the stovetop, then it
Source: Charlie Baggs, Inc.
FIGURE 3.7 Barbecued ribs.
Source: Rosina Food Products, Inc.
FIGURE 3.8 Breaded, stuffed banana peppers.
c03.indd 50 1/14/2016 7:15:24 AM
CHAPTER 3 • Review of Cooking Techniques • 51
is flipped over and the pan is placed in a hot oven to finish the
cooking. It takes practice and experience to judge how long it
takes to finish cooking each product perfectly with this
method, but in a busy restaurant, this approach frees up
stovetop space, which is often in short supply.
Sometimes the best sauce for a dish is one made from the
caramelized juices, protein, and carbohydrates, or fond, left
behind in the sauté pan after the meat is removed after cook-
ing. During cooking, flavorful meat juices (including fats, pro-
teins, sugars, and water) seep onto the hot pan’s surface, where
they cook and harden as the water evaporates, forming “crisps”
of concentrated flavor. It is a shame to let this flavor wash away
with the dishwater! Instead, excess fat, if any, is poured out, the
pan is deglazed with wine, stock, or water, rehydrating and
(with some judicious scraping) solubilizing the caramelized
fond. The liquid is briefly simmered, seasoned, and used as‐is
for an au jus preparation, or else it can be thickened for a more
sauce‐like consistency before serving.
The pan sauce can be thickened by reduction via contin-
ued simmering (as water evaporates, the sauce thickens and
flavors become concentrated), cornstarch slurry (for a more
translucent sauce; is added after the slurry reaches 180–190ºF),
small amounts of roux (a combination of cooked fat and flour
used for thickening opaque soups, gravies, and sauces), beurre
manié (a blend of softened butter and uncooked flour used to
thicken sauces), or even by whisking in small amounts of
chilled whole butter (which thickens and emulsifies via the
presence of small amounts of protein and lecithin in the but-
ter). The result is a rich sauce bursting with flavor.
Additional commercial thickening methods include nu –
merous gums (hydrocolloids), starches, and blends of both
that have been developed to provide viscosity to all types of
liquids in all types of systems and processing conditions
(low pH, high shear, freeze‐thaw, retort stable, etc.). These
can easily be added to commercial products during process-
ing. Raw flour needs to “cook out” when used in a sauce,
which requires extra processing time. On the other hand,
food starches and gums thicken quickly and (usually) don’t
leave an aftertaste. That said, one is often forced to sacrifice
a certain amount of sensory quality for functionality when
developing products for mass production.
Stir‐Frying
Asian cuisine incorporates a quick cooking technique similar
to sautéing. Using slightly more oil and a higher heat than is
traditional with sautéing, small and tender cuts of meats and
vegetables (commonly dredged in cornstarch rather than
flour) are quickly cooked by stir‐frying—typically associated
with the use of a wok. Unlike the flat‐bottomed sauté pan, the
wok is round‐bottomed and bowl‐shaped to facilitate con-
stant stirring. The concept of keeping the food moving when
placed in the hot oil is consistent with sautéing, albeit often
with greater velocity due to the higher heat.
Stir‐frying is a great way to build complex flavors, and the
high temperature and quick‐cooking technique is a great way
to retain natural juices. Once the oil is hot, it is commonly
seasoned with aromatics such as ginger, garlic, scallions, and
chilies. After the oil is seasoned, these ingredients may be
removed or left in as desired. Adding the meat at this stage
continues flavor development and starts the cooking proc ess.
Once the meat is almost cooked, it can either be removed
while the vegetables are cooking and added back at the end
(typically done with meats normally served more rare), or the
vegetables can be added to the meat in the wok while contin-
uously moving the mixture (best used when the meats are to
be cooked well done, such as poultry or shrimp). A splash of
liquid (broth, sherry, soy sauce, etc.) at the end deglazes the
pan, creates a light sauce, and can add another flavor. A corn-
starch slurry may be added if additional thickening is desired.
Pan‐Frying and Deep Frying
Unlike sautéing and stir‐frying, which minimize oil use, pan‐
frying uses enough oil to reach one‐half to two‐thirds the
height of the food being fried. The food is generally larger, like
a pounded pork cutlet or even whole bone‐in chicken parts.
Deep frying and pan‐frying differ mainly in the amount of oil
used. When deep frying, enough oil is used to completely
cover the item being fried. Also, deep frying is more com-
monly used to cook smaller foods—nuggets, strips, fingers,
and the like. A related frying system called broasting involves
deep frying under pressure, and is the “secret” to Colonel
Sander’s Kentucky Fried Chicken. This method is excellent
for preparing crispy but juicy chicken.
In either case, the food is usually coated with some sort of
breading. This can be as simple as seasoned flour or bread
crumbs or can incorporate nuts, cereals, or grains. These coat-
ings help retain moisture, leading to crisp exteriors with
golden colors and tender and juicy interiors. The standard
commercial breading procedure follows a wet, dry, wet, dry
system. Chicken, for example, which is wet by nature, is
dredged in a dry, often seasoned flour mixture (called the pre‐
dust), then dipped in a wet egg‐based mixture to promote
adhesion of the final surface breading (panko, cracker crumbs,
seasoned bread crumbs, and the like). This treatment can be
carried out multiple times to build a thick crust and interest-
ing craggy surface features, but care must be taken not to
overwhelm the product with the breading. Seeding is a proc-
ess of adding a liquid or water to a flour for breading. The flour
will turn into small seed-like shapes; when these are adhered
to the fried item, they provide seeds of crunchy fried flour, a
great way to add texture to a breading system.
A common alternative to breading, especially for deep‐fried
applications, is a batter coating, a semiliquid mixture of flour,
eggs, milk, and sometimes seltzer to provide lightness, used to
coat individual pieces of meat, cheese, seafood, or vegetables.
Unlike breaded foods, which can be stored after breading with-
out frying for some time, battered foods are fried immediately
in order to set the coating quickly and reduce drip loss.
Tempura batter is an especially thin and light batter used in
Japanese cooking. Baking powder is added to the tempura
mixture to add chemical leavening. The tempura batter should
be cold to prevent the batter from reacting before frying.
c03.indd 51 1/14/2016 7:15:25 AM
52 • CULINOLOGY®
Flavors can be added to fried products at several points in
the process: meats can be marinated and seasoned before
coating, and seasonings can be added to the pre‐dust, the egg
wash, the batter, or the crumb coating. As the flavors or
spices tend to flash off the exterior of the food quickly, they
are best applied directly to the product itself before coating or
else in one of the interior coating steps (the pre‐dust or egg
wash) where they will be better protected.
The type of oil chosen also alters the final flavor profile.
Plain shortening, peanut oil, vegetable oil, clarified butter oil,
and rendered meat fats such as lard, tallow, and bacon drip-
pings each add distinctive flavor characteristics to the fried
food. Likewise, old oil that has darkened and is littered with
burnt crumbs, and oil in which strongly flavored items such
as fish have been fried contribute off notes to the fried food.
The lifespan of fryer oil depends on many factors: the type of
oil, how high it is heated, its smoke point, how long it is used,
the volume of food cooked in it, the amount of moisture pres-
ent in the foods, the type of coatings and the amount of salt
used, how well the oil is cleaned and filtered between uses, and
how it is stored. Fats in the presence of oxygen and high heat
(and water, metals, and salt) break down (oxidize), becoming
rancid and bad‐tasting. This is known as fatty acid oxidation.
Therefore, oil should be strained, cooled, and covered after
each use to lengthen its lifespan, or properly discarded when it
is too old. Old oil does not transfer heat as efficiently, and
besides tasting bad and looking dark and unappetizing, foods
fried in old oil absorb more fat because they take longer to
cook.
The smoke point is another consideration when choosing
oil for deep frying. The higher the smoke point, the better the
oil is for deep frying. Typical frying temperatures are from 350
to 400°F (177 to 204°C); therefore, a fat with a smoke point well
above these works best. Fats with lower smoke points break
down more rapidly when exposed to higher temperatures for
extended times, and may ignite as well (not to mention spoil
the food). In general, refined oils that have had impurities
removed have higher smoke points than unrefined oils.
Fats with emulsifiers (such as shortenings intended for
baking), unrefined vegetable oils, and used oil all have lower
smoke points and should not be used for deep frying.
Likewise, unrefined animal fats (lard, tallow) and whole but-
ter decompose quickly under high‐heat conditions and are
better suited to shallow frying and sautéing. Even then, they
should be blended first with oils having a higher smoke point
(and butter should be clarified to remove milk solids that
would otherwise burn). With all of these concerns in mind,
oil manufacturers are able to custom‐blend specially designed
fats specifically for deep frying.
When oils begin to smoke, they also begin to break down,
forming free fatty acids. As these acids build up, they reduce
the oil’s heat transfer efficiency and stability and generate off
flavors. When the triglycerides break down, the oil smokes,
and more of it is absorbed into the product. Other factors
that affect fat absorption are quality of fat, age of the oil,
amount of exposed food surface, type of food, amount of
coating, frying temperature, and length of cook time.
French fries are probably the most popular deep fried food
in the world. In commercial production, the potato strips are
first blanched in water, dried, then dipped in a light batter
coating, par‐fried, and frozen. They are fully cooked later by
deep frying and then held for service. The coating adds extra
crispness and extends shelf life.
Moist‐Heat Cooking
Methods
Moist‐heat cooking methods work by conduction and con-
vection from hot water or other aqueous liquids such as water,
stock, milk, or wine (or steam) to the surface of the food and
then by conduction to the interior of the food. These methods
include boiling, blanching, simmering, poaching, parboiling,
steaming, braising, stewing, combi‐oven cooking, sous vide
(cooking by submersion in a vacuum-sealed pouch), and en
papillote (cooked in an envelope, usually of parchment paper).
Because water transmits heat better than air does, moist‐heat
methods cook foods faster than most dry‐heat methods
(other than frying, which is faster than any moist‐heat method
because oil conducts heat better than water and can be raised
to much higher temperatures than water can). Moist‐heat
cooking methods are essential to gelatinize (tenderize) con-
nective tissue. For this reason, moist‐heat cookery is usually
reserved for less tender cuts of meat. The trade‐off is dilution
of flavor and nutrients and the inability to develop any brown-
ing reaction flavors, colors, or aromas, not to mention crisp
textures. Because the moist heat does not brown the meat
while cooking, a combination cooking technique is used: sear-
ing the exterior of a seasoned meat before beginning the brais-
ing or stewing process. The exception is when your final
product needs to remain white, such as a veal de blanc.
Boiling, Blanching,
and Simmering
Cooking in water is the least complicated of all the cooking
techniques. Foods are simply immersed in heated water and
are cooked to the desired doneness.
Boiling is very effective in cooking many food products,
yet it is inappropriate for delicate items that may fall apart.
One limitation of boiling is that no matter how high the heat
under the pot of water, the temperature of water never
exceeds 212°F (100°C) (at sea level). Boiling is ideal for
starches that must be hydrated and tenderized by the absorp-
tion of water (for example, pasta and potatoes).
Blanching involves completely submerging foods in boil-
ing water for a very short time, then shocking them in ice
water to immediately halt any further cooking. Blanching is
used for quickly setting the color of green vegetables, inacti-
vating enzymes that produce off flavors and degrade color,
and reducing the number of microorganisms. In addition,
blanching forces out dissolved gases in plant tissues, thereby
reducing their volume for easier packaging into containers
for subsequent freezing or canning. Blanching is also used as
c03.indd 52 1/14/2016 7:15:25 AM
CHAPTER 3 • Review of Cooking Techniques • 53
a peeling aid for foods like almonds and tomatoes, and as a
delivery system for functional ingredients (flavors, antioxi-
dants, whiteners, and so on), as in the processing of potatoes
for frozen French fries.
Simmering (slow cooking in barely bubbling liquid) allows
very tough cuts of meat or vegetables to be slowly tenderized
by breaking down (hydrolyzing) complex proteins and carbo-
hydrates, making these foods more tender and palatable.
Simmering is also useful for stock‐making, where slow extrac-
tion of flavor from bones and vegetables without cloudiness
is desired. It is also commonly used for preparing rice and
other grains.
Poaching (Shallow and Deep)
Poaching is a versatile cooking technique, suitable for boneless
chicken breasts, fish, eggs, and fruit. It is often considered a
healthy method of cooking because it does not use fat to cook
the food. There are two types of poaching: shallow and deep.
Both include the cooking of the food in some type of liquid, and
the basic difference lies in the fact that in shallow poaching the
liquid covers roughly one-third of the height of the food, while in
deep poaching the food is almost entirely covered by the liquid.
This poaching liquid (called court bouillon) is usually a
mixture of stock or water; an acidic element, such as lemon
juice, vinegar, or wine; aromatic vegetables; herbs; and spices.
The liquid can be strained or not during poaching.
Shallow poaching is a popular method of cooking that is
usually used with tender fish and poultry breasts. The food is
placed on a bed of aromatic ingredients, then combined with a
few ounces of flavorful liquid, covered loosely, and gently sim-
mered until done. The poaching liquid is often used as a sauce—
for example, sauce vin blanc. After the food item is cooked and
removed from the poacher, the poaching liquid is strained and
reduced and can be finished with cold whole butter to add vis-
cosity, flavor, and mouthfeel. The butter also rounds out the
flavors of the wine or citrus in the poaching liquid.
Deep poaching is completely submerging the food in the
concentrated poaching liquid at a temperature between 160
and 185°F (71 and 85°C). Proteins and vegetables prepared by
this method can be very appetizing; without Maillard brown-
ing, poached foods are paler and have more delicate flavor pro-
files, depending on the flavor impact of the poaching liquid.
The poaching liquid is usually a highly acidulated medium
with herbs and spices. A fish fumé or fish stock can be used to
poach fish, thus building on the fish flavor. Poaching can be
done either on the stovetop or in the oven as long as the water
temperature is closely monitored not to exceed 185°F (85°C).
Otherwise the protein will overcook and become tough and
dry, in spite of being cooked in an aqueous environment.
Poaching eggs is a deep‐poaching technique. The poaching
liquid is on the acidic side, typically from the addition of a
small amount of vinegar, which helps to set the protein in the
egg white. The amount of acid should not be enough to affect
the flavor of the egg.
Individual portions are typical for deep poaching. Cook
times are relatively short; therefore, the cooking liquid must be
flavored quite aggressively to achieve a high level of flavor trans-
fer to the finished product. Aromatic herbs, flavorful broth, and
wine are used to add flavor. Court bouillon is a cooking liquid
specially designed for poaching fish. It is strongly acidic (vine-
gar, white wine, lemon juice) to help set the fish proteins at
lower temperatures (similar to ceviche) so as to keep the texture
extremely tender and prevent overcooking. It is also highly fla-
vored, as there is less time for the cooking food to absorb fla-
vors. Because it is so acidic and strongly flavored, court bouillon
is not reserved after poaching for turning into a sauce.
Par‐boiling and Steaming
Par‐boiling is partially cooking food items in boiling water.
Like blanching, it is a preparatory treatment before a second,
final cook step, but the foods are more fully cooked. In restau-
rants, vegetables, rice dishes, risotto, and pasta are often par-
boiled as part of the mise en place. This reduces the final
cooking period at the time of service and assures proper
cooking and flavor development. A parboiled product is often
sautéed to order. Seasonings are also adjusted to taste at the
time of final cooking.
Steaming is cooking food in superheated water vapor—a
highly efficient method. Properly steamed foods retain more
of their initial volume and many of their water‐soluble nutri-
ents. Many meat processors choose steaming to cook their
products to maximize yield. This is often done in heatproof
bags placed on large rolling racks that fit into walk‐in steam
ovens. Steaming can also be done under pressure. Pressure
increases the attainable temperature of the steam, resulting
in a higher cook temperature and faster cook times than with
atmospheric steam. Steaming is a great benefit when avoid-
ing added calories from fat. When the temperature and time
relationship of steaming are carefully controlled, high‐quality
products are attainable. While it is possible to overcook foods
using steam, the consequences are primarily loss of color and
texture; steamed foods (like all moist‐heat cooked foods)
never burn. Because steaming uses water in its vapor phase,
there is less transfer of beneficial nutrients, pigments, and fla-
vor compounds into the water during cooking. This results in
a more flavorful, colorful, and healthful cooked food.
Steaming is also an excellent process to use in reheating pre-
viously cooked food items, even those prepared using dry‐heat
methods. Steaming previously cooked foods brings them back
up to serving temperature quickly, maintaining the maximum
amount of moisture while reheating evenly in a short period.
While commercial kitchens typically use a steam oven for
steaming, age‐old steaming techniques using stovetop bam-
boo steamers may be used to produce well‐known classic
food items such as tamales (steamed in a cornhusk wrapper),
Greek dolmades (wrapped in grape leaves), fish cooked in
banana leaves, and steamed Asian dumplings.
En Papillote
En papillote (French for “in paper”) steaming is achieved
inside a man‐made wrapper such as parchment paper or alu-
minum foil. The food is enveloped in a pouch completely
c03.indd 53 1/14/2016 7:15:25 AM
54 • CULINOLOGY®
sealed along the edges; the pouch usually contains flavor‐
building ingredients such as herbs, wine, spices, and julienne
vegetables. These additional items, along with the main food,
give off steam when heated. This steam is contained by the
pouch and gently cooks the food within. Delicate foods such
as fish are best cooked this way. One benefit of this method is
that volatile flavor and aroma compounds are trapped inside
the pouch until the pouch is cut open for consumption.
Pressure‐Cooking
Pressure‐cooking (retorting) is a high‐temperature thermal
process that allows water to achieve a higher‐than‐usual boil-
ing point—around 250°F (121°C) at 15 psi. Because the tem-
perature is higher, this process can quickly cook foods that
normally take a long time to cook. This process is also widely
used in the canned food industry to ensure that botulism
spores are killed. The time and temperature are determined by
the type of product and package being processed. Some foods,
such as broths, have rapid heat transfer and require shorter
processing times. Products that are denser, or contain partic-
ulates that can stick together (such as thick soups or stews)
require a longer cook time to ensure the product is heated
to the appropriate safe temperature all the way through.
Container size and material (metal, glass, plastic) have a criti-
cal impact on process time, as does the acidity of the product;
process time to control microbial growth is shorter for acidic
foods with a pH below 4.6 (the pH below which pathogens do
not survive) and much longer for foods above pH 4.6. Low‐pH
foods create a less favorable growth environment for bacteria
and hence require less thermal processing.
Braising and Stewing
Braising encompasses two cooking methods and is generally
reserved for tougher cuts of meat with plenty of connective
tissue. First the item is seared (dry heat) in a bit of oil to cre-
ate a dark brown color and to develop the complex flavors
and colors produced by the Maillard reaction. After the meat
is seared, mirepoix and a tomato product (if used) are also
seared to develop additional flavor compounds.
The meat is then submerged two‐thirds in a liquid, the
cooking vessel is covered, and the product is simmered
(moist heat) until fork tender. The juices from the meat pro-
vide some of the moisture, but most is added separately in
the form of water, stock, wine, or juices. The complex flavors
created by the dry‐heat browning step solubilize in the cook-
ing liquid and add a depth of flavor and color that otherwise
would be absent. Typical braised items are lamb or veal shank
(osso buco), short ribs, pot roast, and oxtail.
Because meat cuts best for braising are tough and full of
connective tissue, they are slowly cooked in a low to moder-
ate oven (300 to 325°F [149 to 163°C]) for an extended period.
Smaller items are braised for a shorter time (2 to 3 hours, or
until fork tender). Larger items are braised for 4 to 5 hours
(for example, large, tough pieces of meat from the shoulder,
shank, and leg quarters).
During the moist cooking phase, the tough connective tis-
sue, collagen, is converted to gelatin, the meat becomes suc-
culent and tender, and the sauce begins to thicken both from
evaporation and also from the gelatin that is produced.
Stewing differs from braising mainly in the size of the item
cooked. In general, stews are made from bite‐size pieces of
meat that are completely submerged in the stewing liquid. Also,
cook times are shorter due to the smaller size of the items being
cooked. Examples of stewing are chili, beef stroganoff, beef
stew, and beef bourguignon. Note that stews and braises con-
taining “white” meats (chicken, veal) are not typically seared
before simmering as browning is not desired in these cases.
Immersion Cooking (Sous Vide)
Immersion cooking or sous vide cooking is the process of
cooking food, sealed under vacuum (usually in a heat‐proof
plastic bag), in a temperature‐controlled water bath. Sous
vide literally translates as “under vacuum,” which is the key to
this cooking method. This moist‐heat cooking method brings
distinct advantages because the vacuum bag prevents escape
of volatile flavors and aromas, and allows the food to gently
cook in its own juices. The method also eliminates the pos-
sibility of overcooking because food is cooked in water main-
tained at the exact same temperature as the desired finished
product’s final temperature. Take for example, a vacuum‐
packed seasoned piece of meat immersed in a bath of water
maintained at 145°F (63°C) and circulated to ensure even
temperature. Regardless of how long the meat is kept in this
heated water, it will never be cooked beyond medium done-
ness, and the entire piece of meat will be cooked to the same
degree. Understandably, because the temperature variance
between the food being heated and the heating medium is
low, the heating times can be very long. However, the longer
cooking times enable virtually the complete breakdown of
connective tissues, producing a very tender, perfectly cooked
end product. In addition, flavors and nutrients are trapped
inside the bag and can easily be turned into a delicious and
nutritious sauce. Of course, because this is a moist‐heat
proc ess, caramelization and Maillard browning do not occur.
If those flavors and colors are desired, the meats must be Source: Charlie Baggs, Inc.
FIGURE 3.9 Braised meat.
c03.indd 54 1/14/2016 7:15:32 AM
CHAPTER 3 • Review of Cooking Techniques • 55
seared before being placed in the vacuum bag. Likewise, if a
surface crust is desired ( for a steak, for instance), searing can
also take place after removal from the bag at the conclusion
of sous vide cooking. In this case, the meat should be blotted
dry before searing. With sous vide cooking, it is essential to
use proper procedures and well‐maintained equipment
because the food product spends a large amount of time in
the microbial growth danger zone (40 to 140°F [4.5 to 60°C]),
and anaerobic bacterial spores from Clostridium botulinum
can germinate if proper temperature control is not main-
tained. Therefore, sous vide foods must be refrigerated or fro-
zen for storage if not immediately consumed. As a result of
this concern for safety, the USDA and many local health
departments require restaurants and food processors who
wish to use this process to be fully trained and certified.
Thickeners
In addition to cooking method and flavor development,
sauce texture is next in importance to delivering a rewarding
eating experience. A variety of thickeners are used as impor-
tant tools in the creation of a great dish, and it is important to
understand them and why and how they work. Commonly
used thickening methods include roux, reductions, starches,
gelatin, purees, and emulsions. Each of these provides dis-
tinctive characteristics and mouthfeel.
Roux
A roux is a combination of equal parts by weight of fat and
flour that are cooked to remove the raw cereal taste of the
flour. The cooked roux is then added to sauces to thicken
them. Typically, all‐purpose flour is used for roux because
its starch content is higher than that of durum flour or
bread flour. The fat portion of the roux can come from any
source; oil or clarified butter are most commonly used in
the restaurant kitchen. However, many chefs choose a fat
whose distinctive flavor enhances the dish, for example,
duck fat or bacon grease. Roux is cooked in a sauté pan or
skillet for varying lengths of time, depending on the color
and flavor desired. White, blond, brown, and black roux are
the traditional descriptions. Each is cooked a little longer,
further caramelizing the starches in the flour while render-
ing them less able to swell and hydrate. The darker the roux,
the more is needed to thicken the same volume of liquid.
Thickening with roux is simply adding and hydrating the
starch molecules contained in the flour. The fat serves as a
medium to cook the flour, but it also separates each small
granule of starch so it can be incorporated into the sauce with-
out clumping. Starch swelling of roux made with all‐ purpose
flour begins when the liquid reaches about 155°F (68°C).
Reductions
A reduction is the simplest and oldest form of concentrating
and thickening sauces or broths; it also captures more of the
flavor and clarity of the original ingredients without added
flavors from the addition of other thickeners. The process of
evaporating the water from a sauce or gravy results in an
increase in the concentration of solids. This results in a thick-
ening effect by increasing the level of solids in suspension; it
also concentrates the flavor compounds and salts that are in
the solution. Because of this, it is better to season to taste
once the sauce or broth is reduced. Examples of reductions
include demi‐glace, tomato sauce and ketchup, maple syrup,
cream reductions, and fruit jellies.
Starches
Starch slurries (starch suspended in a liquid, usually water)
are used in a wide variety of applications to provide rapid
thickening. Starches can be extracted from a wide variety of
source materials, including corn, wheat, potato, rice, and
tapioca. Each type has its own functional benefits and brings
different character to the sauce. For instance, wheat starches
develop much more opacity in a sauce than does cornstarch,
which yields a translucent sauce.
Modified food starches are manufactured with functional
qualities that are custom‐designed for nearly any application.
Starch manufacturers can custom‐craft starches to tolerate
different levels of shear, freeze‐thaw stability, high or low pH,
and hydration temperatures. For example, native “cook‐up”
starches require being transformed into a slurry before being
added to a hot liquid to prevent the starches from clumping
up. The liquid then needs to be heated so that the starch
granules can begin taking up water (gelatinize) and provide
thickening. Modified “cold set” (“instantized” or pre‐
gelatinized starches) do not require being made into a slurry
or heating to work. They have already gone through the gelat-
inization process by the starch processor, then have been
dried for shelf stability and ease of use.
Gelatin
Gelatin is a thickening agent that is virtually odorless, color-
less, and tasteless. Formed from the hydrolysis of collagen,
this protein, when dissolved in water and heated, forms a
matrix that entraps water. Gelatin forms a reversible gel (dis-
cussed in Chapter 2). It is unique in that it creates more vis-
cosity the colder the product becomes. This is particularly
useful when a food must be solid at room temperature but
pourable when hot. Gelatins are commonly used in cold des-
sert applications. That said, small amounts of gelatin added
to a sauce will also provide viscosity and mouthfeel.
Pureeing
Using the process of pureeing to thicken is accomplished by
the mechanical breakdown of cooked solids along with some
or all of the liquid in which they were cooked. Purees can be
chunky or silky smooth, depending on the desired texture,
and pureeing is accomplished through the use of high-speed
blenders, commercial food processors, homogenizers, or
mills of various types. Examples of commercial purees
include applesauce, mashed potatoes, pesto, hummus, and
all types of baby foods.
c03.indd 55 1/14/2016 7:15:32 AM
56 • CULINOLOGY®
Conclusion
In summary, it is critical that today’s research chef and food
product developer understand the fundamentals of cooking
techniques, how to choose the right methods to achieve the
desired results, and how each method impacts the product.
This combined expertise is what we know today as
Culinology®. It leads to greater food development efficiency,
quality, speed to market, and profitability for the processor,
and better‐tasting, more nutritious, and affordable foods for
consumers. This collaboration of chefs and scientists, of art
and science, is fundamental to the future of food product
development.
c03.indd 56 1/14/2016 7:15:32 AM
CHAPTER 4 • Protein‐Based Foods: Introduction and Red Meats • 57
Introduction to Protein
Chemistry
High‐quality protein is a key nutrient in a healthy diet. It is a
major part of the skin, muscles, organs, and glands, where it
provides structure, support, and cellular repair and helps
regulate many functions of the body. Immunity, digestion,
muscle contraction, wound healing, and reproduction all rely
on proteins for proper function.
Nutritional Properties
of Proteins
Amino acids are the basic building blocks of proteins and
the nutrients necessary for normal bodily functions. Twenty
amino acids are found in proteins, but only nine are consid-
ered essential to adult humans: phenylalanine, valine, threo-
nine, tryptophan, isoleucine, methionine, leucine, histidine,
and lysine. Cysteine (or sulfur‐containing amino acids), tyro-
sine (or aromatic amino acids), and arginine are additionally
required by infants and growing children. 1 Essential amino
acids are so called because the body cannot synthesize them,
which makes it necessary to include them in one ’ s diet.
The quality of a protein is measured by its amino acid con-
tent. Proteins that do not contain all the essential amino
acids are considered incomplete proteins . All animal‐derived
proteins (except for gelatin), including eggs and milk, are
complete proteins . Most vegetable‐derived proteins (with
few exceptions) are incomplete, usually lacking in either
lysine (grains) or methionine (legumes). In each case, the
missing essential amino acid is called the limiting amino
acid . A well‐balanced diet is essential to meet dietary require-
ments, particularly when following a vegetarian diet. Failure
to include even one of the limiting amino acids in the diet can
result in serious health consequences.
Animal‐derived proteins are often considered nutrition-
ally superior to plant proteins. That said, abundant contro-
versy regarding the harvesting of meat is based on personal
preference, religious beliefs, ethical issues, and environmen-
tal concerns. However, meat protein continues to play a
major role in the American diet.
Although most plant proteins do not contain all of the
essential amino acids, through careful planning and selection
the consumption of complementary vegetables can provide a
highly nutritious diet. Complementary vegetables, when eaten
over the course of a day, can provide all the essential amino
acids needed to maintain a nutritionally balanced diet.
The body requires a high degree of net protein utilization
(NPU), which is the ratio of amino acids converted into pro-
tein versus the amount of amino acids actually consumed.
When consuming complete proteins, the body is able to use
the amino acids to synthesize all of the critical structural and
functional proteins required for the maintenance of good
health. When consuming incomplete proteins, the body con-
verts the amino acids into energy (calories) rather than using
them for protein building. Thus, they are unavailable for all
of the critical protein‐dependent life functions the body
depends on.
If, however, a variety of incomplete proteins is consumed
within the same day (e.g., beans and rice), they become com-
plementary (beans lack methionine but supply lysine; rice
lacks lysine but supplies methionine), and the body is able to
recognize the entire meal as a complete protein. Called pro-
tein complementation , this strategy is essential for the main-
tenance of good health in individuals who eat a predominantly
vegetarian diet.
Once consumed, proteins are broken down (hydrolyzed)
by acids and enzymes (other proteins) in the stomach. First,
hydrochloric acid hydrolyzes proteins into long‐chain pep-
tides , and then stomach enzymes reduce these peptides to
di‐ and tripeptides. One of the more commonly recognized
protease enzymes (enzymes that break down proteins only)
in the stomach is pepsin. Finally, enzymes located in the
small intestine (such as trypsin) hydrolyze the di‐ and tripep-
tides to individual amino acids, which are then absorbed by
the small intestine.
James W. Lamkey, Ph.D., Technical Services , Spicetec Flavors and Seasonings
4
Protein‐Based
Foods: Introduction
and Red Meats
c04.indd 57 1/22/2016 12:59:00 PM
58 • CULINOLOGY®
The nutritive value of a protein is not only related to its
amino acid content but also its overall digestibility. This is
measured by comparing the total amount of protein con-
sumed with that excreted in feces. Eggs, for example, are 98
percent digestible, while wheat protein (gluten) is 91 percent
digestible.
There are five current methods for assessing protein qual-
ity. The Amino Acid Score (AAS) is a basic measure of protein
quality, but it is not adjusted for the digestibility of the pro-
tein, which depends on the type of protein (animal proteins
are more digestible than plant proteins) as well as on the
method of food preparation. If digestibility is factored in, the
AAS will be somewhat lower than stated. An updated version
of AAS, and the one most widely used, is the Protein
Digestibility Corrected Amino Acid Score (PDCAAS). As the
name implies, a digestibility factor based on fecal protein is
added to the AAS, giving truer results. The next three meth-
ods, Protein Efficiency Ratio (PER), Biological Value (BV),
and Net Protein Utilization (NPU), are all based on feeding
studies with rats. There is some concern among researchers
as to how well rat studies reflect the needs of growing human
infants and the maintenance of human adults.
Although soybeans are typically limited in the amino acid
methionine, soy protein is commonly touted as a complete
plant protein. According to the PDCAAS system, soybeans
have a score of 0.91 while soy protein isolate has the maxi-
mum score of 1.0 (the same as egg whites), followed by milk
and meat proteins.2 Other plant‐based sources of complete
protein receiving growing recognition and achieving growing
popularity include quinoa and amaranth, grains common in
Latin America.
Protein Structure
Proteins are differentiated by their primary, secondary, ter-
tiary, and sometimes quaternary structures, which influence
functionality in the body and in foods. The primary structure
is defined as the unbranched sequence of amino acids that
comprises the backbone of proteins. This sequence is unique
to each type of protein, within species as well as between
species. The secondary structure is defined by patterns of
hydrogen bonds between the backbone amino acids. The
most common secondary structures are alpha helix (coiled
arrangement), which is the format typically shown when
illustrating DNA structures, and beta sheets (uncoiled
arrangement). Examples of beta sheet proteins are collagen
and connective tissue.
The tertiary structure, the further folding of the protein, is
greatly influenced by the primary structure. Depending on
the tertiary structure, proteins can be classified as globular or
fibrous. Globular proteins (spheroproteins) are generally
water soluble and folded. Globular proteins do not provide
structure. They are primarily enzymes, transporters, and reg-
ulators of everyday functions. In aqueous environments,
hydrophobic (water‐fearing) groups are located on the inside
of the fold while the hydrophilic (water‐loving) groups are
extended into the aqueous environment. In a hydrophobic
environment, one that is dominated by oil or fat, these pro-
teins refold so the hydrophobic groups are exposed while the
hydrophilic groups are protected.
The second type of tertiary structure is identified as
fibrous; it is found in animals only. Fibrous proteins (sclero-
proteins) are rod‐like in shape and have the primary function
of providing support and locomotion. They are usually water
insoluble but can be solubilized with weak salt solutions
(such as brines or marinades) under certain process condi-
tions. They can then be used as water binders and texture
builders.
The quaternary structure is how proteins interact with
other proteins. Not all proteins have quaternary structures.
One that does is hemoglobin, which has four identical but
individual protein units grouped together with the ultimate
goal of transporting oxygen to the muscles and organs of the
body (hemoglobin is responsible for the color of blood). Each
subgroup has an affinity for oxygen that diminishes as
another subgroup entraps oxygen within its matrix. This
diminished affinity for oxygen allows the fully saturated
H2N
R
H
C COOH
FIGURE 4.2 Basic chemical structure of amino
acids, with R representing various side groups.
FIGURE 4.1 Protein quality comparison chart.
Protein Type PDCAAS AAS PER BV % Digestibility
Whey Protein 1.00 1.14 3.2 100 99
Whole Egg 1.00 1.21 3.8 88–100 98
Casein 1.00 1.00 2.5 80 99
Soy Protein Concentrate 1.00 0.99 2.2 74 95
Beef Protein 0.92 0.94 2.9 80 98
Wheat Gluten 0.25 0.47 NA 54 91
Source: Whey Protein Institute (http://wheyoflife.com/consumer/requirements).
c04.indd 58 1/14/2016 11:24:26 AM
http://wheyoflife.com/consumer/requirements
CHAPTER 4 • Protein‐Based Foods: Introduction and Red Meats • 59
hemoglobin to release its entrapped oxygen molecules to
myoglobin, which in turn delivers the oxygen to the muscles
and organs of the body. Myoglobin is responsible for the color
of meat.
Functional Properties
of Proteins
Having both hydrophobic and hydrophilic regions makes
proteins excellent stabilizers of high‐fat emulsions (mayon-
naise) and foams (whipped cream) and as binders (bread
dough). Proper processing procedures, such as optimal tem-
perature and agitation, assist with the unfolding of the pro-
teins to expose the hydrophobic groups, allowing them to
interact with oil, fat, air, or other denatured proteins in the
emulsion while the hydrophilic side chains interact with the
aqueous component in the emulsion. It is the association of
these proteins in stable networks that provides the desired
functionality.
The protein’s environment also has a significant impact
on its functionality in foods. This environmental influence is
primarily related to how the protein hydrates (absorbs water)
and retains (or loses) water throughout the manufacturing
and/or cooking process. In many cases, we want to retain as
much of the moisture as possible, as in the case of roasts,
steaks, or chops. In other cases, as with salamis and pepper-
oni, we want to reduce the moisture level under controlled
conditions to improve color, texture, and shelf life. The simple
task of cooking a steak is in reality a chemical process that
changes the protein structure to improve tenderness, palat-
ability, and digestibility, and that causes chemical reactions
between proteins and sugars to create a more desirable color
and flavor. Product developers can provide the right kind of
environment to get the optimal value from any protein.
Keep in mind that any change in protein structure is con-
sidered denaturation because the protein is being taken out
of its natural state. This may be temporary if the environment
reverts to its original state or the processing is gentle. On the
other hand, if the environmental change is permanent or the
processing is severe, the structural change may become per-
manent. At this point, denaturation turns into coagulation,
which in terms of cooking is irreversible and evidenced by
curdling.
The most influential environmental characteristics affect-
ing the structure and function of protein are pH and heat.
The acidity of a substance is measured in terms of pH. As pH
drops, the overall environment becomes more acidic. As pH
increases, the environment becomes more basic.
Amino acids, the building blocks of proteins, are ampho-
teric compounds that contain both basic (amine) groups
and acidic (carboxyl) groups. This makes proteins good
buffers that help aqueous systems resist sudden changes
in pH.
Because of its unique combination of amino acids, each
protein has both positive and negative charges that change as
pH changes. When an amino acid loses a proton (a positively
charged particle), the protein becomes more negatively
charged. When an amino acid gains a proton, the protein
becomes more positively charged. The point where the num-
ber of positive charges equals the number of negative charges
is called the isoelectric point. Each type of protein has its own
isoelectric point; this can be used as an analytical tool to sep-
arate and identify proteins.
In a solution with a pH below (more acidic than) the iso-
electric point, the protein carries a net positive charge.
Conversely, as the pH rises above (becomes more basic than)
the isoelectric point, the net charge on the protein becomes
negative. The impact of this change in net charge becomes
apparent when we take into account one of the laws of nature:
Like charges repel, while opposite charges attract. At the iso-
electric point, attraction between the charges is strongest.
This means the protein molecule is closed and very tightly
folded. As pH moves higher than the isoelectric point, the
number of negative charges increases, resulting in greater
repulsion between the amino acids. This repulsion along the
backbone of the protein opens up the structure, allowing for
more moisture retention, which in turn improves solubility.
Therefore, the solubility of a protein is lowest at its isoelectric
point. Note: Very rarely does meat go below the isoelectric
point. At some point the acid starts to hydrolyze the protein
and functionality is lost.
Knowing these characteristics of proteins helps proces-
sors manipulate the functionality as well as the texture of a
protein through pH modification. A marinade containing
alkaline phosphates, for example, increases the pH of the
meat above the isoelectric point, causing greater repulsion
between the amino acids and resulting in greater moisture
retention. Alternatively, fermentation of meat creates sau-
sages (such as pepperoni) by increasing the level of acid in the
meat, thus moving the pH closer to the isoelectric point,
causing greater attraction between the amino acids and
thereby reducing the protein’s ability to retain moisture. This
reduces water activity (aw) and extends shelf life without the
need for refrigeration.
Three types of protein are found in muscle foods: myo-
fibrillar, which makes up the bulk of muscle tissue (what we
characterize as meat); sarcoplasmic; and connective tissue.
In the processing of meats, each protein type plays a role in
the overall desirability of the finished product.
Sarcoplasmic proteins are soluble in water or very low
ionic strength salt solutions (weak brines). This group con-
tains a variety of proteins, some related to color, some to ten-
derness, and others to the overall function of the muscle
tissue. Of these proteins, those responsible for color and
H N
N
H OR
H O R
O HC C
C C
FIGURE 4.3 A dipeptide comprised of two
amino acids.
c04.indd 59 1/14/2016 11:24:26 AM
60 • CULINOLOGY®
tenderness are most important. Myoglobin is a sarcoplasmic
protein and, as the major pigment in red meat, is responsible
for changes in color. Enzymes also belong to this category
and help break down other proteins for improved tenderness.
The second group of functional proteins in meat are the
stromal proteins. They include collagen, elastin, and reticu-
lin, collectively referred to as connective tissue. Col lagen is
the most abundant protein in the body, as it is present in
muscle, skin, and bone. In many meat cuts, the collagen is
trimmed and discarded to make the meat more palatable.
However, in certain cuts of meat, primarily meat from mus-
cles used for locomotion, the collagen cannot be trimmed
without losing too much of the meat to be economically fea-
sible (yield loss). It is therefore up to the cooking conditions
to improve the tenderness of these cuts. These types of
meats may be cooked with moist heat to break down the
collagen, which turns into gelatin, thereby tenderizing the
cut. This is the basis for braising and stewing.
Myofibrillar proteins are the third type of functional
protein. With the highest level of functionality in meat,
these proteins are primarily responsible for moisture
retention and texture (to maximize yield and enhance
eating quality). These are also known as the salt‐soluble
proteins; as the name implies, these proteins can be
extracted from meat using salt. The addition of salt allows
the meat filaments to absorb water and thus increase
meat weight, volume, and f lexibility. Salt dramatically
enhances the water‐holding capacity and the stickiness of
the meat. The salt‐soluble proteins combine with water
through both direct molecular interactions (adsorption)
and physical entrainment (absorption). The sticky mari-
nated meat forms a binding glue‐like layer of extracted
proteins on its surface; this denatures or coagulates upon
heating and forms a rigid, firm mass. This is the principle
used in preparation of many formed meat products such
as chicken nuggets or meat loaves. The three proteins of
primary interest are actin, myosin, and actomyosin, the
proteins responsible for muscular contraction and relaxa-
tion. Myosin has the best water‐holding capacity and emul-
sifying properties, followed by actomyosin and finally actin.
Levels of protein organization
Primary protein structure
is sequence of a chain of amino acids
Alpha helix
Amino acids
Alpha
helix
Pleated
sheet
Pleated sheet
occurs when the sequence of amino acids
are linked by hydrogen bonds
occurs when certain attractions are present
between alpha helices and pleated sheets.
is a protein consisting of more than one
amino acid chain.
Secondary protein structure
Tertiary protein structure
Quaternary protein structure
Source: National Institutes of Health. National Human Genome Research Institute, “Talking Glossary of Genetic Terms,” accessed April 23,
2015, http://www.genome.gov/glossary/y/?id=169.
FIGURE 4.4 Basic structure of proteins.
c04.indd 60 1/14/2016 11:24:26 AM
http://www.genome.gov/glossary/y/?id=169
CHAPTER 4 • Protein‐Based Foods: Introduction and Red Meats • 61
Muscle Structure
Skeletal, smooth, and cardiac muscles are the three types of
structural muscles found in food animals, with skeletal mus-
cle comprising the majority of meat. Cardiac muscle is found
only in the heart. Smooth muscle comprises the organs and
membranes of the body.
Skeletal muscle, the primary functional component of
meat, is also referred to as striated muscle because of its
appearance, which is banded when viewed under a micro-
scope. The striations in skeletal muscle are caused by sar-
comeres, the individual contractile units of muscle. The
appearance is the result of alternating dark and light areas
caused by the various proteins responsible for contraction.
The primary proteins involved in contraction are actin and
myosin, the combination of which forms actomyosin.
Contraction occurs when myosin, stimulated by a nerve
impulse, pulls the actin filament into the center of the sar-
comere, resulting in a shortening of the sarcomere.
Myofibrils are composed of multiple sarcomeres, the
length of which are determined by the size and function of
the muscle with which they are associated. The sarcomeres
contract unilaterally, resulting in the contraction of the
myofibril. Muscle fibers, which are the cellular structures of
muscle, are composed of multiple myofibrils. Muscle fibers
in turn form muscle bundles and, ultimately, whole muscles.
All of the muscle fibers within a muscle work in unison when
stimulated by nerve signals to perform the work requested.
Meat
Meat is commonly recognized as the flesh of animals used for
food. Usually the term refers to skeletal muscle, but it also
includes other edible tissues such as the organs—liver, brains,
tongue, kidneys, tripe (stomach), chitterlings (intestines),
sweetbreads (pancreas and thymus), and skin (pork rinds).
Offal is the general term for edible internal organs. Although
generally we think of meat as referring to mammalian species
(cows, pigs, sheep, and goats), technically speaking, fish and
poultry provide “meat” as well.
Whatever its source, meat has been a substantial part of
the human diet from earliest times. Initially, humans
resorted exclusively to hunting to acquire meat. Then,
approximately 7500 years ago, at the time of the earliest
civilizations, domestication of meat animals began. This
included sheep in Egypt and Mesopotamia from 3500 to
3000 BC, pigs in Hungary as early as 2500 BC, and cattle, also
in Mesopotamia, from as early as 5000 BC. Selective cattle
breeding that resulted in certain animals bred for meat pro-
duction and others for milk production did not occur until
the mid‐1700s.
Meat consists of approximately 75 percent water, 19 per-
cent protein, 2.5 percent fat, 1.2 percent carbohydrates,
and 2.3 percent non‐protein substances (minerals and free
amino acids). All muscle tissue is very high in complete pro-
tein, containing all of the essential amino acids, and is also
a good source of zinc, vitamin B
12
, selenium, phosphorous,
niacin, vitamin B
6
, iron, and riboflavin. Meat is low in carbo-
hydrates and does not contain any dietary fiber. While each
kind of meat has about the same content of protein and car-
bohydrates, fat content varies widely depending on the spe-
cies and breed of animal, the way in which the animal was
raised (including what it was fed), the anatomical part of
the body, and the methods of butchering and cooking.
Fat contributes most of the calorie content of meat and is
relevant to concerns about dietary health, particularly
because mammalian animal fat is saturated and contains
cholesterol.
Overconsuming large quantities of food, including meat,
can have adverse effects on health, including obesity, heart
disease, hypertension and diabetes. In recent years, health
concerns have been raised about the consumption of meat
increasing the risk of cancer. In particular, red meat and
processed meat were found to be associated with higher risk
of cancer, particularly cancer of the colon. The complexity of
cancer limits our ability to be absolutely sure about cause and
effect for any behavior in which a person participates. The
consumption of meat is one way to achieve the nutritional
requirements a body needs. Maintaining a healthy weight
Muscle fiber
Nuclei
Myofibril
Myofibril (2,500 per fiber) 8,000 sarcomeres per myofibril
Sarcomere (basic contractile unit)
Thin myofilament Z-lineH-zone
M-line
Thick myofilament
I-BandA-Band
I–BandA–Band
I-Band
Source: Texas A & M University, accessed September 24, 2015,
http://meat.tamu.edu/ansc‐307‐honors/structure‐muscle.
FIGURE 4.5 The contractile proteins of muscle.
c04.indd 61 1/14/2016 11:24:27 AM
62 • CULINOLOGY®
and a healthy lifestyle are the key ingredients to reduce the
risk of chronic diseases, including cancer.
Meat Production
Meat is produced by killing livestock animals and harvesting
their edible parts. The age at the time of slaughter varies
depending on the animal as well as the target market for the
meat. As a general rule, beef destined for retail and foodser-
vice markets is harvested from cattle that are 24 to 30 months
of age. Veal, on the other hand, is from cattle harvested at 6 to
8 months of age, which is similar for pigs and lambs. Many
processors vary this time depending on the cost of feed and
other environmental conditions, taking a risk that they will
not get the full value of the carcass.
A calf increases in size tenfold from a birthweight of 100
pounds to 1000 pounds at the time of harvest. The first six
months after birth, calves live on mother’s milk and grass.
Then the calf is weaned from the cow and dines mostly on
grass with some grain supplementation. For grass‐fed ani-
mals, this scenario plays out until the day of harvest.
Alternatively, at 18 months of age, grain‐fed cattle are started
on a highly concentrated, nutrient‐balanced diet consisting
of corn and soybeans for 180 days, at which time they are
harvested.
The fat from grass‐fed cattle may have a yellow tinge that
comes from the large quantities of carotene in grass. The fat
from grain‐fed cattle is almost white in color. Whether the fat
from grass‐fed beef provides significant nutritional benefits
over grain‐fed is still a controversial subject, especially as
most consumers trim the majority of fat from their beef
before eating it.
Because of the higher calorie content of grain, grain‐fed
cattle generally lay down more marbling (intramuscular fat
deposits) than the same animal on a grass diet. More mar-
bling means the animal can receive a higher quality grade
(prime, choice, select, etc.). The higher the quality grade, the
higher the price paid for the carcass. Although some breeds
of animals can become sufficiently marbled while eating
grass, a grain diet allows more fat to be deposited. This means
cattle that are genetically unable to become highly marbled
on a grass diet achieve more marbling and a higher quality
grade on a grain diet.
One of the main differences between cattle and pigs is their
ability to digest cellulose. Pigs have simple stomachs, much
like humans, and cannot digest cellulose. Cattle, on the other
hand, are ruminants and have very complex digestive sys-
tems. For that reason, cattle can use grass as a source of nutri-
ents while pigs cannot. Other animals that are ruminants
include sheep, goats, and deer (venison).
Harvesting Muscle Proteins
Each step in the harvesting of meat is designed to assure high
quality, worker safety, and, ultimately, safe and healthy food
for consumers. Time, temperature, good sanitation practices,
and condition of the animal are all important to maximizing
the quality of the protein being harvested.
Immobilization, or stunning, is the first step of the harvest
process. This renders the animal unconscious so it does not
feel pain or become a safety hazard to individuals during the
rest of the slaughter process. Immobilization is accomplished
by one of three methods: physical, electrical, or modified
atmosphere.
Physical immobilization is normally used for large animals
such as cattle, while electrical and modified atmosphere
methods are used for smaller animals. The physical method is
a high‐impact force delivered to a targeted spot on the head,
usually delivered by a mechanical device such as captive bolt
or plastic bullet. This renders the animal unconscious.
Electricity, when applied to an animal’s body, sends elec-
trical currents through the muscles, causing violent contrac-
tions. These can cause bones or blood vessels to break,
resulting in blood spotting. It is important for proper settings
to be used so these defects are minimized.
Use of modified atmosphere techniques, such as vacuum
chambers or carbon monoxide, can reduce the defects that
occur with electrical stunning. Oxygen is removed until the
animal is unconscious. As a result, there are no violent con-
tractions that could damage the meat.
The process of converting muscle to meat begins when
the supply of oxygen to the muscles and major organs is lost.
For terrestrial animals, this begins with the removal of blood,
known as exsanguination. When blood is removed, not only
the supply of oxygen but also the ability to remove cellular
waste products is lost. Without oxygen, anaerobic fermenta-
tion becomes the primary source of energy for the muscles,
which are still trying to maintain homeostasis (the body’s
physiologic status quo). As a result, lactic acid, a major by‐
product of anaerobic fermentation, begins to accumulate in
the muscles. Without blood to remove it, the buildup of lactic
acid causes the overall pH of the meat to decline and become
more acidic. Muscle pH is normally close to neutral (6.2 to
7.2), while meat, under normal conditions, has a pH in the
range of 5.7 to 6.2, depending on the species, the muscle
within the animal, and the ultimate concentration of lactic
acid. Extenuating circumstances could make the ultimate
pH higher or lower. This could have a tremendous impact on
the appearance and functionality of the protein.
If an animal is overly excited for a short period (1 to 2
hours) before slaughter, large quantities of lactic acid will be
produced in the muscle. This also occurs under times of heavy
exercise. (The pain or burn you feel in your muscles after
lengthy exercise or running is the result of high levels of lactic
acid.) If lactic acid is still present at the time of exsanguina-
tion, circulating blood is no longer available to remove it, and
the pH of the meat drops rapidly to a point lower than it would
be normally. A rapid drop in pH when the temperature of the
muscle is still above 90°F (32°C) results in a breakdown of the
protein and loss of functionality. The meat will look very pale,
be soft to the touch, and release moisture easily (be exuda-
tive). Pork and turkey are more prone to this condition, which
is called PSE, for pale, soft, and exudative. In hogs, this is a
genetic condition that was inadvertently selected when pigs
were bred for leanness. As pigs became leaner, they also
c04.indd 62 1/14/2016 11:24:27 AM
CHAPTER 4 • Protein‐Based Foods: Introduction and Red Meats • 63
became more prone to PSE. Over time, however, geneticists
were able to separate the two characteristics and breed for
leanness while reducing the incidence of PSE meat.
Rigor mortis is a stiffening of the muscles as they transform
into meat. This stiffening is the result of the muscles going
through the process of final contraction after death. When
energy production in the body ceases after death, the muscles
remain contracted, causing the carcass to stiffen. Over time,
this contraction is relaxed due to the action of proteolytic
enzymes located within the muscles. This relaxation is called
resolution of rigor and is part of the aging process for beef.
The time for rigor mortis to be completed in animals dif-
fers and can be related to the size of the animal. Beef requires
up to 24 hours to complete rigor, while pork completes in 6 hours
or less.
For red meat and poultry, the time for onset of rigor mor-
tis and its completion can be reduced through a process
called electrical stimulation, whereby an electrical impulse
is passed through the hot carcass immediately after slaugh-
ter. Another advantage of electrical stimulation is tenderiza-
tion of the meat, as fractures across muscle fibers occur that
help tenderize muscles.
Electrical stimulation also reduces the incidence of cold
shortening, which occurs when a muscle is cooled too rapidly
after slaughter. The carcass is chilled immediately after
slaughter to prevent spoilage, but if the carcass is chilled too
rapidly (to less than 60°F [15.5°C] before the completion of
rigor mortis), the result is cold shortening and subsequent
toughness.
If the carcass is frozen before completion of rigor mortis,
the result is thaw rigor and subsequently extremely tough and
dry meat due to excessive drip loss. Muscle contraction is con-
trolled by the release of calcium from within the muscle. When
a nerve impulse signals for contraction, a controlled release of
calcium ensues, which initiates the contraction of the muscle.
If the muscle is frozen before the completion of rigor mortis, ice
crystals damage the storage vessels holding the calcium ions.
Upon thawing, the calcium is released en masse, causing a
catastrophic contraction that can break bones in smaller ani-
mals such as lamb. It is therefore important for rigor mortis to
be completed before the meat is frozen.
The next step in the harvest process is the removal of
hide or hair. In the case of beef and lamb, the hide is first
removed from the head and then the head is removed from
the carcass before the rest of the hide is removed. In the case
of red meat, the lymph nodes are inspected by the U.S.
Department of Agriculture’s Food Safety and Inspection
Service (USDA‐FSIS) inspector. It is important the head and
carcass remain identified until the inspection is completed.
If any abnormalities are found, the carcass is separated from
the others and disposed. Edible components removed with
the head can be used for further processing. Head and cheek
meat are often used in the manufacture of sausage. These
sources of protein are of good quality but have high amounts
of connective tissue, which limits the ways they can be used.
The tongue and, possibly, brains are also removed and
harvested for sale.
Removal of the hide is designated a critical control point
(in the HACCP food safety management system) due to the
microbial load that may be associated with the hide. Extra
care is taken not to transfer any dirt and debris to the carcass.
Where excessive debris is present, the animal is taken
through a washing process to eliminate extraneous material
before harvest.
Some hog carcasses have all or a portion of the hide or
skin removed, but this is usually reserved for carcasses to be
used in sausage manufacturing. For most hog carcasses, just
the hair is removed, leaving the hide on the carcass to help
retain moisture. Hair is removed by scalding the carcass in
140°F (60°C) water for 4 minutes, which softens the connec-
tive tissue surrounding the hair follicle and releases the folli-
cle from the skin. The carcass is then placed in a dehairing
machine, which contains numerous paddles that pull the
hair from their follicles. For harder‐to‐reach areas of the car-
cass, a flame singes the remaining hairs, which are then
removed by scraping. The objective of the singeing is to make
the finer, lighter‐colored hairs more visible. Then the carcass
is either trimmed or washed to remove debris.
Removal of the internal organs, or viscera, is known as
evisceration. Care is taken not to tear or otherwise open the
digestive tract so as not to expose the carcass to feces. If
digestive material contaminates the carcass, that portion is
cut out and discarded. Most of the evisceration process is
automated for poultry but continues to be manual for red
meat processing.
Next, the carcass is prepared for the cooling process. It is
washed, and microbial interventions such as steam or organic
acids may be applied to the surface. This does not sterilize the
carcass but reduces the number of microorganisms to more
manageable levels. Larger carcasses, such as beef, veal, and
pork, are split lengthwise to facilitate cooling. Smaller car-
casses, such as lamb and poultry, are left whole. Cooling rates
are monitored by inserting a thermometer in the thickest por-
tion of the carcass. It is recommended that carcasses are
placed in the cooler within 1 hour of exsanguination and
reach an internal temperature of 40°F (4.4°C) within 24 hours.
USDA Inspection
It is mandatory for red meat and poultry sold in the United
States to be inspected for wholesomeness by either state or
federal inspectors. Currently, 27 states have inspection pro-
grams equal to but separate from the federal inspection pro-
gram. Any processor in a state that offers a state meat
inspection program can choose to harvest meat under that
program but is then restricted to selling within that state.
Federally inspected meat products, on the other hand, can be
sold throughout the United States and in some other coun-
tries as well.
The inspection of a meat carcass at the point of harvest is
for wholesomeness only. Trained inspectors, supervised by
veterinarians, inspect the organs and lymph nodes (early
indicators of disease) to determine if the meat is fit for human
consumption. This inspection, which also includes observing
c04.indd 63 1/14/2016 11:24:27 AM
64 • CULINOLOGY®
the animal before slaughter, gives the inspector information
about the animal’s overall condition at time of harvest. If an
animal (or carcass) shows signs of disease, it is cut from the
herd, and a practicing veterinarian later determines whether
it is fit for human consumption. This inspection process is
funded through federal income taxes, although ongoing dis-
cussions consider whether it should be paid for by the proc-
essors instead.
Once the animal and carcass are inspected for wholesome-
ness and deemed suitable for human consumption, each pri-
mal cut (the basic large sections of the carcass from which
steaks, roasts, and other meat portions are cut) is stamped to
indicate that the carcass has been inspected and passed.
Included on the stamp is a unique number that identifies the
specific meat‐processing facility where the meat originated.
Meat Grading
Unlike meat inspection, which is mandatory, meat grading is
a voluntary program paid for by the processors. Grading is a
government program instituted to assure uniformity in the
meat industry.
Beef
Approximately 65 percent of steer (castrated male) and
heifer (female cow that has never borne a calf ) carcasses are
graded. Because this is a voluntary program that costs the
processor money, only those carcasses destined for foodser-
vice or retail, where higher margins can be made, are graded.
Once the grade is assigned, the grader marks the carcass
with a roller stamp. The remaining carcasses are not graded
and are referred to as no roll because they do not carry a
grade stamp. No‐roll carcasses, which may still contain high
quality meat, are sold to processors for commercially pre-
pared foods, grinding, or other manufactured products.
Many meat processors have their own brands and rely on
the identity of the brand name (e.g., Angus Beef ) for sales
rather than the USDA grade.
There are seven possible grades for beef based on age and
marbling score: Prime, Choice, Select, Standard, Commercial,
Utility, and Canner. Prime, Choice, Select, and Standard
grades are based on cattle less than 42 months of age. In 2009,
3 percent of beef was graded Prime, 63 percent was graded
Choice, 33.5 percent was graded Select, and 0.5 percent was
graded Standard. Commercial, Utility, and Canner grades are
assigned to cattle over 42 months of age. Most beef animals
destined for harvest are less than 30 months of age.
Maturity, a trait based on carcass characteristics rather
than chronological age, is assessed by visually evaluating the
Inspection Stamp for raw meat
Inspection Stamp for processed meat and poultry
Inspection Stamp for raw poultry
INSPECTED
AND PASSED BY
DEPARTMENT OF
AGRICULTURE
U.S.
U.S.
EST.38
P-42
U. S.
3 8IN
S
P
’ D & P
’S
’D
I
N
SP
ECTE
D
* *
Source: USDA, http://www.fsis.usda.gov/factsheets/inspection
_&_grading/index.asp.
FIGURE 4.6 Inspection stamps found
on carcasses and packages.
Degrees of
Marbling
Maturity
Standard
Select
Choice
Prime
Cutter
Utility
Commercial
A B C D E
Slightly
Abundant
Slightly
Abundant
Moderate Moderate
Modest Modest
Small Small
Slight Slight
Traces Traces
Practically
Devoid
Practically
Devoid
Degrees of
Marbling
Source: USDA.
FIGURE 4.7 Degrees of marbling. The relationship between maturity and marbling.
c04.indd 64 1/14/2016 11:24:27 AM
CHAPTER 4 • Protein‐Based Foods: Introduction and Red Meats • 65
level of ossification along the backbone and the color and
texture of lean muscle tissue. Bone begins as a connective tis-
sue framework, and as the animal ages calcium fills in the
network in a process called ossification, to make a hard struc-
ture. The tips of the spinal processes (buttons) along the
backbone are the last to ossify and therefore are used as indi-
cators of maturity. Lean tissue tends to get darker and devel-
ops a coarser texture as the animal matures.
Marbling is assessed by trained individuals who estimate
the amount of fat between the muscle fibers (intramuscular
fat). Subcutaneous fat (under the hide) and seam fat (between
muscles) are not considered in this evaluation. Marbling is
the last type of fat to be generated as the animal is growing.
Although genetics plays a large role in the amount of mar-
bling, it also comes from the feeding regimen. Grain‐fed cattle
are given a highly concentrated diet composed primarily of
corn. Animals that are more prone to storing intramuscular
fat (marbling) have a greater chance of doing so on a grain
diet. Grass does not provide as much caloric energy as grain;
therefore, marbling does not develop as readily in grass‐fed
animals, although some cattle breeds can develop more
marbling and achieve higher quality grades on grass.
Experienced cattle raisers are able to identify by breed and
body type an animal’s potential to grade choice or higher.
Beef carcasses can also be graded for yield. Yield grades
are an indication of the expected amount of retail cuts that
can be obtained from the carcass. Yield grades are numbered
from 1 to 5, with 1 indicating the highest amount of yield. The
grades for beef are based on the amount of fat measured over
the rib eye muscle (longissimus dorsi) between the twelfth
and thirteenth rib, the amount of fat around the kidney, pel-
vis, and heart (KPH fat), and the size of the rib eye between
the twelfth and thirteenth rib. Larger rib eyes result in higher
yield grades (lower numbers), while more fat gives rise to
lower yield grades (higher numbers). As might be expected,
better yield grades coincide with higher quality grades.
The primary consideration for grading is to obtain a con-
sistent product at the quality desired. Some organizations
market their product based on quality and consistency. Certain
breeds deposit marbling at a much faster rate than others. The
Angus is known for its well‐marbled and high‐quality meat.
The Angus Association has taken advantage of these traits and
formed the Certified Angus Beef (CAB) program to create
specifications for meat that ensure a consistent, high‐quality
product that commands a premium price. This approach has
certainly succeeded if the number of restaurants and super-
markets advertising CAB is any measure of success.
Pork
Pork grading is based solely on yield without regard to age or
marbling. The grades assigned to pork carcasses range from
1 to 4 and are indicative of the yield based on the four lean
cuts (ham, loin, Boston butt, and picnic). Fat over the rib eye
and the size of the rib eye between the tenth and eleventh rib
are also used for yield grade assessment. In addition, an eval-
uation of the amount of muscling in the ham is evaluated,
with higher levels indicative of higher yields. As with beef
yield grades, the lower the number, the higher the yield.
Utility grade can also be assigned to pork; this indicates the
carcass has unacceptable quality characteristics such as soft
and oily fat, pale, soft, exudative, lean, or a too‐thin belly.
Utility‐grade pork is used for ground items such as pork sau-
sage, scrapple, and lunch meat as well as for pet food.
Lamb
Lamb, like beef, is graded for both quality and yield. Although
yield measurement is basically the same—taking into
FIGURE 4.8 Quality and yield grades per species.
Species Quality Grades Yield Grades
Beef Prime, Choice,
Select, Standard,
Commercial, Utility,
Cutter, and Canner
1, 2, 3, 4, 5
Lamb and
yearling mutton
Prime, Choice,
Good, Utility, and
Cull
1, 2, 3, 4, 5
Mutton Choice, Good,
Utility, and Cull
1, 2, 3, 4, 5
Veal and calf Prime, Choice,
Good, Standard,
and Utility
NA
Barrows and gilts US No. 1, 2, 3, 4,
and Utility
Sows US No. 1, 2, 3, 4,
and Utility
Source: USDA.
FIGURE 4.9 States with state meat and/or
poultry inspection systems.
Alabama
Arizona
Delaware
Georgia
Illinois
Indiana
Iowa
Kansas
Louisiana
Maine
Minnesota
Mississippi
Missouri
Montana
North Carolina
North Dakota
Ohio
Oklahoma
South Carolina
South Dakota
Texas
Utah
Vermont
Virginia
West Virginia
Wisconsin
Wyoming
FIGURE 4.10 Sex classes of animals used for food.
Intact
Male
Castrated
Male
Older
Female
Young
Female
Beef Bull Steer Cow Heifer
Pork Boar Barrow Sow Gilt
Lamb Ram Wether Ewe Ewe
Source: James Lamkey, Ph.D.Source: James Lamkey, Ph.D.
c04.indd 65 1/14/2016 11:24:27 AM
66 • CULINOLOGY®
account the variables that affect overall yield of the primary
cuts—quality grades are slightly different. In lamb, quality is
based on age, fat streaking, and conformation of the carcass.
Conformation takes into account the development of the
muscle and skeleton, particularly around the leg and along
the back. Fat streaking is evaluated in the flank, which is an
indirect method of evaluating the marbling. Age is deter-
mined at the time of slaughter based on the break joint of the
front shanks. The break joint is the epiphyseal plate just
below the spool joint; it is still soft in lambs and breaks easily.
As the animal matures, this plate, which is the point at which
bone lengthens, becomes more ossified and more difficult to
break. Lambs have break joints on the front shank, yearlings
have either break joints or spool joints, while mutton (mature
animals older than one year) have spool joints.
Meat Color
The pigment responsible for color in red meat is a water‐
soluble protein called myoglobin, which accepts oxygen from
hemoglobin in the blood and transfers it to muscle tissue for
normal biological functions. The amount of myoglobin in
muscle tissue varies between species and is also dependent
on the age of the animal and the location of the muscle.
Muscles used for locomotion tend to have a higher concen-
tration of myoglobin than do muscles used for support. This
is best exemplified by comparing the breast muscle from
chicken to that of wild fowl. Because wild fowl regularly fly,
their breast muscle has a high concentration of myoglobin
and is close to beef in color. In comparison, chickens rarely
fly, and if they do it is for short distances. The muscles in
chicken wings and breasts require less myoglobin, resulting
in whiter muscle. Beef generally has more myoglobin than
pork, which has more myoglobin than poultry.
Color is the first attribute of red meat used to determine
quality because myoglobin is very responsive to changes in
the environment. One of the characteristics of myoglobin is
its ability to exist in three different states depending on the
amount of oxygen to which it is exposed. In the absence of
oxygen, myoglobin becomes a purple color known as deoxy-
myoglobin (deoxygenated myoglobin), a characteristic of red
meat that is first cut or that has been packaged under vac-
uum. The colors most people associate with freshness in
meats—the bright cherry‐red color of beef and the pinkish
color of pork—are due to oxygenated myoglobin, known as
oxymyoglobin. These two states of myoglobin are readily
reversible as oxygen is removed or added to the myoglobin
molecule. For example, the cut surface of meat first looks
purple, but if exposed to air it gradually turns red.
When fresh meat ages and begins to turn brown, the third
state, metmyoglobin is the predominant form. Whereas
deoxymyoglobin and oxymyoglobin can fluctuate if oxygen is
connected to or disconnected from myoglobin’s oxygen‐bind-
ing site, metmyoglobin is formed by the oxidation of the iron
contained within the pigment and is not easily reversed.
Keep in mind that the bright cherry‐red color of beef occurs
when the myoglobin molecule is oxygenated, while the brown
color of metmyoglobin occurs when the molecule has oxi-
dized. In living muscle, metmyoglobin‐reducing activity
(MRA) is based on a group of enzymes that can reverse the
oxidation reaction, taking metmyoglobin back to either the
deoxymyoglobin or oxymyoglobin state. In meat, however,
these enzymes degrade over time, making the formation of
metmyoglobin a permanent state; this is an indicator of meat
age and sensory quality. That said, meat with a brown color is
not necessarily unsafe for consumption.
The change in the color of red meat during cooking is com-
monly used by chefs and consumers as indicators for the
degree of doneness. Rare meat is associated with a cool red to
purple center, while meat that is well done can be gray or
white in color, depending on species. Although these color
changes are well established, they are not recommended as
the sole indicator of doneness. From a food safety standpoint,
the only true indicator of doneness is temperature. Beef has a
much higher myoglobin concentration than pork, making the
changes in the color of beef easier to identify. While beef trans-
forms from red to pink to brown during normal cooking, pork
is more subtle and turns from pinkish‐red to less pink to white.
A number of factors can influence the appearance of
cooked meat. When the pink color persists in meat even
though it has been cooked to the proper endpoint tempera-
ture, consumers suspect the meat is underdone. This can be
a particular problem with poultry and pork, meats that are
traditionally cooked and eaten well done. High pH (which
may be the result of adding phosphate to the meat to increase
water‐holding) causes a slower change in the appearance of
myoglobin as temperature increases, giving the appearance
of the meat not being cooked properly. Meat with a low pH
(which may be due to acidic marination) tends to brown
faster, giving an appearance of being done when in fact it has
not had sufficient heat treatment.
Nitrite contamination can also give meat an undercooked
appearance by retaining the pink color in the meat. Nitrite is
the chemical compound used in the curing of meat products
such as ham and bacon. The pigment that results from myo-
globin reacting with nitrite is nitrosyl hemochrome, which
produces a permanent pink color.
Exposure to carbon monoxide (CO) also gives a pink color
to meats, although this is usually identified as the smoke ring
in barbecue circles and is a desirable characteristic. However,
open flames, whether gas or charcoal, can be the cause of car-
bon monoxide production and cause pinking in red meats
and poultry.
Bacterial spoilage has also been shown to influence the
color of cooked meat.
Ground meats, by their nature, can have all three forms
of myoglobin present due to both internal and external oxy-
gen exposure. Premature browning can result because oxy-
myoglobin and metmyoglobin turn brown more readily
when heated,3 making the product appear fully cooked
when in fact it has not achieved the appropriate tempera-
ture to kill pathogens. Deoxymyoglobin is more resistant to
heat and changes color in a predictable manner during
cooking.
c04.indd 66 1/14/2016 11:24:27 AM
CHAPTER 4 • Protein‐Based Foods: Introduction and Red Meats • 67
Food quality issues are more commonly associated with
whole muscle products, but food safety issues are the greater
concern with ground meat products. With whole muscle prod-
ucts, the interior is not contaminated unless the surface of the
meat is penetrated; therefore, it is easy to destroy surface bac-
teria through the cooking process. With ground products,
however, the very act of grinding has the potential to mix bac-
teria throughout the product. Without reaching proper inter-
nal temperatures, bacteria can survive the cooking process. To
be completely sure proper temperatures are attained, ther-
mometers should be used to check the internal temperature.
Channels of Distribution
Meat packers are businesses that harvest animals grown for
meat. Purveyors are businesses that buy primal cuts from
packers and cut the meat into wholesale and retail cuts for
the retail and foodservice industries. Processors take raw
materials from the packers and further process them into
products such as hams, frankfurters, roast beef, and sau-
sages. Some retail supermarket and big‐box chains buy pri-
mals directly from the packer and cut the meat themselves.
In the late 1970s and early 1980s, the industry shifted from
shipping animal carcasses to shipping boxed meat, an inno-
vation started by a company then known as IBP (now known
as Tyson). Where purveyors and retail outlets once had to
deal with marketing the entire carcass and disposing of waste
fat and bone, the advent of boxed meat allowed them to buy
only the wholesale cut needed, usually boneless, trimmed to
specification, and vacuum packaged. More than 90 percent of
the beef in this country is merchandized as boxed beef.
Consolidation in the meat industry has also streamlined
meat channels so that many packers are also purveyors and
processors, selling product directly to foodservice and retail
outlets. In some cases, the processor prepares retail cuts at a
central location and ships to stores to save time and labor.
Boxed beef and veal is usually based on the primal cuts of
the carcass: chuck, rib, loin, round, plate, brisket, and flank.
Currently, retailers and foodservice companies can buy
subprimals (smaller cuts of meat obtained from the larger
primal cuts of an animal) based on specifications outlined by
International Meat Product Specifications (IMPS).
Pork processors quickly followed suit, offering pork sub-
primals vacuum packaged and shipped in boxes. Pork loin,
ham, belly, and shoulder are the four primals offered to retail-
ers and processors. The ham and belly are often further proc-
essed into cured ham and bacon, respectively. Loins are used
to create pork chops and roasts. Shoulders are often sepa-
rated into the shoulder picnic and shoulder butt. The picnic
can be cured to provide a smoked picnic. The shoulder butt is
often offered as a pork roast. Both can be used for trimming
to make sausage products. Note: Only cured meat from the
ham (the back leg and buttock) can legally be called ham.
Even though meat from the shoulder (picnic and butt) can be
cured to look and taste exactly like ham, it cannot be labeled
as such. It can only be labeled picnic ham, smoked shoulder, or
smoked butt.
Lamb primals—leg, loin, rack, and shoulder—are also
offered boxed. The most popular retail lamb cuts are shoulder
blade chops, loin chops, rack of lamb, shoulder chops, lamb
leg, and lamb rib chops.
Meat that has been recently harvested and has been main-
tained at a temperature below 40°F (4.4°C) and above 28°F
(2.2°C) is considered fresh. One exception to this is poultry,
which is normally cooled to 26°F (–3.3°C) for distribution,
putting a slight ice crust on the surface.
Wholesale red meat is usually distributed in plastic bags
under vacuum (generally known as Cryovac®, although this is
actually a trade name of the Sealed Air Corporation). Storing
meat under vacuum removes the oxygen required for the
growth of spoilage microorganisms, resulting in extended
shelf life. In most cases, vacuum‐packaged wholesale cuts
have a shelf life of 120 days or more. However, the removal of
oxygen results in the meat becoming purple as myoglobin
reverts to the deoxymyoglobin state.
Meat can also be stored and distributed under modified
atmosphere packaging (MAP). This system begins in much
the same way as vacuum packaging, as all the air is removed.
However, a special mixture of gases (combinations of carbon
dioxide, nitrogen, and oxygen) is put back into the bag (via gas
flushing), thus modifying the atmosphere inside the bag. MAP
helps optimize shelf life, flavor, and appearance by reducing
oxidation and the growth of spoilage bacteria. In some cases,
the modified‐atmosphere package is referred to as a pillow pack
when the package appears to have excessive air inside. Pillow
packs are more often used for processed meat and consumer
packaged meat rather than wholesale meat cuts to maintain
the integrity of the slices and keep them from sticking together.
One recent addition to MAP is carbon monoxide (CO),
which has long been used in Europe as part of MAP packag-
ing. Although carbon monoxide is toxic to breathe at high
levels, it is considered safe at the levels used in packaging.
Flushing packaged raw meats with carbon monoxide allows
for the retention of the characteristic cherry‐red color of
fresh meat beyond what would be normal because CO binds
more tightly to myoglobin than does oxygen while still pro-
viding the color effects of oxygenation. The downside is that
meat can retain a fresh appearance well beyond the stage
where it is no longer wholesome for consumption. In this
case, strict attention must be paid by both the packer and the
consumer to package dating.
Aging of Beef
It is well known that aging beef creates a product that is supe-
rior in both texture and flavor to unaged beef. This is not true
for other protein‐based products (seafood, poultry, pork).
What makes beef so different?
Most domestically raised meat animals are slaughtered
when less than 1 year of age; many are less than 6 months of
age. Beef, on the other hand, comes from animals more than
1 year old and more typically approaching 24 months of age.
As an animal ages, connective tissue increases and becomes
less soluble, causing a reduction in tenderness of the meat.
c04.indd 67 1/14/2016 11:24:27 AM
68 • CULINOLOGY®
Enzymes are inherent in meat and become active a short
time after harvest, usually due to the release of calcium as the
cells start to die. These enzymes, collectively identified as
proteases (enzymes that break down proteins), begin to break
down the protein, resulting in more tender meat. This is the
initiation of the aging process. The longer the aging time, the
more tender the meat. However, during the aging process,
microorganisms actively grow on the surface of the meat if it
is not handled properly. While certain microorganisms are
desirable for flavor development, those responsible for spoil-
age or safety issues must be controlled.
There are two methods for aging meat. The older method
is dry aging, where the meat is aged unwrapped and exposed
to the air, while wet aging is relatively new (being associated
with the development of boxed beef ) and takes place while
the meat is enclosed in its vacuum package.
Temperature, relative humidity, air movement, and general
sanitation of the area where the meat is dry aged are essential
considerations in successfully aging beef. Temperature of the
aging room should be maintained at 34 to 36°F (1 to 2°C), rela-
tive humidity at 85 to 90 percent, and an air flow of 15 to 20
linear feet per minute at the surface of the product.
The area where the aging takes place should be cleaned
and sanitized before and during the process. Beef cuts are
perishable, and many factors can contribute toward spoilage.
Aside from poor sanitation in the area causing bad odors to
occur, storing any other strongly scented product in the room
can cause off‐flavor development. Excessive aging results in
an accumulation of microorganisms. Their presence in large
numbers results in a slimy‐appearing surface, as the most
likely places for microorganisms to grow are on the moist
surfaces. If these contaminated areas are not completely
trimmed off and discarded during processing, the end prod-
ucts of microbial growth will produce undesirable flavors and
odors in the finished product.
Shrinkage occurs during dry aging because the meat is
unwrapped and exposed to moving air. In fact, shrinkage
(due to dehydration) is necessary in order to concentrate fla-
vor components in the beef, increasing its desirability. The
longer the aging period, the greater the total loss in weight
due to dehydration and the need for trimming of surfaces
that have dried excessively or have detectable microbial
growth. As a result, dry‐aged beef is much more expensive
than wet‐aged beef due to the large amount of dehydration
and trim loss.
Wet aging is accomplished by leaving vacuum‐packaged
cuts of meat under refrigeration for an extended period
before sale. As in dry aging, the primary objective is to tender-
ize the meat. However, the meat resulting from this type of
aging has a different flavor and texture than dry‐aged beef
because it does not get dehydrated, and the organisms that
grow under vacuum are different from those that grow in air.
The microbes that grow on the surface of dry‐aged meats are
aerobic organisms (including bacteria and molds), whereas
anaerobic bacteria, which produce lactic acid, primarily grow
in vacuum‐packaged meats. This lactic acid production helps
with the tenderizing process and contributes to overall flavor.
Because there is no moisture loss, yields are higher and
therefore cost to both the processor and the consumer is
lower.
Common Concerns
in the Meat Industry
Bovine Spongiform
Encephalopathy
Bovine spongiform encephalopathy (BSE), also known as
mad cow disease, belongs to the family of transmissible
spongiform encephalopathies (TSE)—rare progressive neu-
rodegenerative disorders that affect both humans and ani-
mals. TSEs are distinguished by long incubation periods,
characteristic spongiform (sponge‐like) appearance of the
brain associated with neuronal loss, and a failure to induce
inflammatory response. BSE is a chronic, degenerative dis-
ease that affects the brain and central nervous system in
adult cattle after a long incubation period; it is progressive
and always fatal. The disease is not caused by a virus or bac-
terium but is thought to be the result of a pathological change
in the structure of a naturally occurring brain protein, the
prion protein. A prion is a transmissible agent that is able to
induce abnormal folding of cellular proteins in the brain,
leading to brain damage and the characteristic signs and
symptoms of the disease. The change makes the prion pro-
teins very resistant to degradation and extremely infectious,
thus enabling transmission of the disease.4
Other TSEs include scrapie (affecting sheep and goats),
chronic wasting disease (CWD, affecting deer and elk), trans-
missible mink encephalopathy (TME), and feline spongiform
encephalopathy (FSE). However, BSE is the only one found in
an animal raised for human consumption where transmission
of the disease to humans has been seen (known as a
“zoonose”).
The spread of the disease has been exacerbated by the
practice of feeding cattle (which are herbivores) with feed
containing mammalian nervous systems (typically from
other infected cattle).
BSE has been detected only in the brain, spinal cord, bone
marrow, and retina of affected cattle. No BSE agents have been
detected in muscle tissue/meat, milk, or blood. Although wide-
spread in Europe, to date the United States has seen only three
documented cases of BSE‐infected cows, the last one in 2006.5
There are human forms of these diseases (Creutzfeldt‐
Jakob Disease [CJD]; Gerstmann‐Straussler‐Scheinker syn-
drome [GSS]; fatal familial insomnia [FFI]; and kuru, which is
seen in certain cannibalistic tribes), but variant CJD (vCJD) is
the only one caused by ingestion of BSE‐diseased cattle. The
pathologies of all of these diseases are the same, with exten-
sive irreversible degenerative changes to the brain leading to
death. Among humans, as of October 2009,6 the total world-
wide number of confirmed vCJD deaths is 213, including 167
in the United Kingdom, 25 in France, 5 in Spain, 4 in Ireland,
3 each in the United States and the Netherlands, 2 in Portugal,
c04.indd 68 1/14/2016 11:24:28 AM
CHAPTER 4 • Protein‐Based Foods: Introduction and Red Meats • 69
and 1 each in Italy, Canada, Saudi Arabia, and Japan. There has
never been a case of vCJD that did not have a history of expo-
sure within a country where the cattle disease BSE was occur-
ring. There is no known treatment for vCJD, and it is invariably
fatal.7
U.S. agencies have acted quickly with precautionary steps
to prevent BSE in cattle and vCJD in humans from spreading.
These steps include:
• Prohibiting importation of live ruminant animals and
most ruminant products from all of Europe (USDA)
• Examining U.S. cattle exhibiting abnormal neurological
behavior to test for BSE (USDA)
• Prohibiting the use of most mammalian protein in the
manufacture of animal feeds given to ruminant animals
(FDA)
• Recommending that animal tissues used in drug prod-
ucts not come from a country with BSE (FDA)
• Issuing guidelines asking blood centers to exclude
potential donors who have spent six or more cumulative
months in the United Kingdom between 1980 and 1996
from donating blood (FDA)
• Conducting regular surveillance for cases of vCJD among
humans (Centers for Disease Control [CDC])
• Conducting research on BSE, CJD, vCJD, and related
neurological diseases (National Institute of Health
[NIH])
Parasites: Trichina spiralis
Trichina spiralis is a roundworm (nematode) parasite that
can live in the muscles of animals such as pigs and rats, and
when ingested by humans can cause the disease trichinosis.
Poor management practices were one of the main reasons for
high incidences of Trichina spiralis in pigs raised in the United
States. Many years ago, pigs were primarily fed garbage and
other scraps, which were the primary source of the parasite.
Modern methods use corn and soybeans as the primary feed
for commercial hog operations, with tight controls on vermin
(mice and rats) infestation. Until recently, consumers were
instructed to only eat pork products that were cooked well
done to assure the parasite was killed. Now, chefs and other
food preparers can cook pork to a lower degree of doneness,
resulting in a juicier and more tender product.
Note: Besides cooking, freezing is also an acceptable
method for eliminating Trichina in pork. The USDA’s Code of
Federal Regulations requires that pork intended for use in
processed products be frozen at 0°F (–17.8°C) or below for a
minimum of 106 hours. Curing (application of salt, nitrites,
smoking, and/or drying) and irradiation are also effective
ways to render Trichina noninfective.
Bacterial Concerns
The CDC has reported that about 48 million people—one in
six Americans—get sick, 128,000 are hospitalized, and 3000
die annually from foodborne diseases.8 Meat products, which
are an excellent source of nutrients for bacterial pathogens,
can be a major source of foodborne illnesses. These, however,
can be prevented with proper handling, processing, and stor-
age. Proper handling of meat begins with the live animal and
must not end until the product is consumed. Each step must
set up hurdles so that microorganisms of concern do not
cause illness.
Antibiotics are used in the livestock industry to prevent
disease, which is important for both animal and human
health. Farmers and ranchers believe that not treating cattle
that become sick is inhumane. The use of antibiotics in the
meat industry is controversial because of their effects on
microbial resistance. Antibiotics used in beef cattle produc-
tion must go through a rigorous testing process before being
approved by the Food and Drug Administration (FDA). The
Center for Veterinary Medicine (CVM), a branch of the FDA,
is responsible for ensuring that animal drugs are safe, effec-
tive, and manufactured to the highest quality standards.
While there are many aspects that go into the animal antibi-
otic approval process, human safety is a key component.
Withdrawal times are established as part of the approval
process to specify the number of days that must pass between
the last antibiotic treatment and before the animal can enter
the food supply. This ensures that an antibiotic has suffi-
ciently cleared an animal’s system. By law, any person admin-
istering antibiotics to livestock must follow withdrawal
periods. Additionally, the FDA and U.S. Department of
Agriculture (USDA) have a coordinated surveillance program
to monitor for antibiotic residues.9
Four organisms are of primary concern to the meat indus-
try: Campylobacter jejuni, Salmonella (various subspecies),
Escherichia coli (especially E. coli O157:H7), and Listeria
monocytogenes. The following information is taken from the
Centers for Disease Control and Prevention.10
Campylobacter jejuni
• Campylobacter jejuni is the most commonly reported
bacterial cause of foodborne illness (FBI) in the United
States. The CDC estimates (2011) that C. jejuni causes
approximately 845,000 illnesses, 8400 hospitalizations,
and 76 deaths in the United States each year. Most
cases of Campylobacter infection occur as isolated,
sporadic events, and are not usually a part of large
outbreaks.
• C. jejuni is a curved gram‐negative rod that grows best at
107°F (41.67°C) and low oxygen concentrations. These
characteristics are ideal for growth in its normal habitat—
the intestines of warm‐blooded birds and mammals.
• It can be found in retail poultry. Produce may also
become contaminated with Campylobacter if it is ex-
posed to raw meat or poultry products or juices in the
kitchen, or if it is contaminated by animal feces in the
fields where it is grown. Campylobacter is also com-
monly found in raw milk and dairy products. However,
the source is unknown for more than 50 percent of
campylobacteriosis infections.
c04.indd 69 1/14/2016 11:24:28 AM
70 • CULINOLOGY®
• Campylobacter causes gastroenteritis with fever, bloody
diarrhea, abdominal pain, malaise, nausea, and vom-
iting two to five days after ingestion; symptoms last a
week. In a small number of cases, Campylobacter infec-
tion can also cause a rare disease called Guillain‐Barré
syndrome, which occurs several weeks after the acute
diarrheal illness and may result in permanent
paralysis.11
• Campylobacter readily develops antibiotic resistance
from continuous exposure to antibiotic‐treated chicken
feed.
• Campylobacter is easily destroyed by cooking; the pri-
mary modes of transmission are through consumption
of unwashed raw or undercooked foods and cross‐con-
tamination whereby uncooked foods come into contact
with contaminated surfaces. Ingestion of as few as 500
organisms—an amount that can be found in one drop
of chicken juice—has been proven to cause human
illness.12
Salmonella Species
• Salmonella is the second most commonly reported
cause of bacterial FBI, with approximately 30,000 con-
firmed cases yearly in the United States. (The CDC esti-
mates (2011) that over 1 million cases occur annually in
the United States, but the majority go unreported). Of
these cases, approximately 20,000 result in hospitaliza-
tion, and 378 result in death. This means that Salmonella
accounts for almost 30 percent of foodborne illness–
related deaths each year.
• Salmonella can cause three kinds of illness: gastroenteri-
tis, typhoid fever, and bacteremia (infection of the
blood). Symptoms of salmonella gastroenteritis include
diarrhea, abdominal cramps, fever, nausea, and/or vom-
iting. In mild cases, the diarrhea may be non‐bloody,
occur several times per day, and not be very voluminous;
in severe cases, it may be frequent, bloody and/or mucoi-
dal, and of high volume. Fever generally occurs in the
100–102°F (37.8–38.9°C) range. Whereas the diarrhea
typically lasts 24 to 72 hours, patients often report
fatigue and other nonspecific symptoms lasting seven
days or longer.
• An infectious dose of Salmonella is incredibly small,
between 15 and 20 cells.
• Salmonella is found in meats, poultry, eggs (even inside
the shell!), milk and dairy, seafood, sauces and salad
dressings, cake mixes, cream‐filled desserts, and so on.
Escherichia coli (E. coli)
• E. coli is a normal inhabitant of the intestinal tract of
humans and other warm‐blooded animals and birds. It
is the dominant bacterial species found in feces.
Newborns have a sterile alimentary tract, which within
two days becomes colonized with E. coli.
• Most kinds of E. coli bacteria do not cause disease in
humans. Normally, E. coli serves a useful function in the
body by competitively suppressing the growth of harm-
ful bacterial species in the gut and by synthesizing
appreciable amounts of vitamins.
• The CDC estimates that every year at least 75,000
Americans are sickened, 2000 are hospitalized, and
about 60 die as a direct result of E. coli infections and its
complications.
• More than 700 serotypes of E. coli have been identified.
Those that are responsible for the numerous reports of
outbreaks traced to the consumption of contaminated
foods and beverages produce Shiga toxin (Stx), so
called because it is virtually identical to that produced
by another bacterium known as Shigella dysenteriae
type 1 (which also causes bloody diarrhea and hemo-
lytic uremic syndrome [HUS]). Shiga toxin is one of
the most potent toxins known to man, so much so
that the CDC lists it as a potential bioterrorism agent.
• The best‐known and most notorious Stx‐producing
E. coli is E. coli O157:H7. Stx‐producing E. coli do not
sicken the animals that carry it. The animals are merely
the reservoir for the bacteria.
• Although E. coli O157:H7 is responsible for the majority of
human illnesses attributed to E. coli, additional Stx‐pro-
ducing E. coli can also cause hemorrhagic colitis and
post‐diarrheal hemolytic uremic syndrome (D+HUS).
HUS is a syndrome that is defined by the trilogy of hemo-
lytic anemia (destruction of red blood cells), thrombocy-
topenia (low platelet count), and acute kidney failure. It
can lead to permanent kidney failure and death (it is the
number‐one cause of kidney failure in children). In about
10 percent of E. coli cases, the Shiga toxin results in HUS.
• Stx‐producing E. coli organisms are hardy and can survive
several weeks on surfaces such as countertops and up to a
year in some materials like compost. They have a very low
infectious dose, meaning that a relatively small number of
bacteria (fewer than 50) are needed to cause infection.
• The CDC estimates that 85 percent of E. coli O157:H7
infections are foodborne in origin.13 In fact, consumption
of any food or beverage that becomes contaminated by
animal (especially cattle) manure can result in contract-
ing the disease. Foods identified as sources of contami-
nation include ground beef, venison, sausages, dried
(noncooked) salami, unpasteurized milk and cheese,
unpasteurized apple juice and cider, orange juice, alfalfa
and radish sprouts, lettuce, spinach, and water. Pizza
and cookie dough have also been identified as sources of
E. coli outbreaks.
• It is important to note that only about 10 percent of
infections occur in outbreaks; the rest are sporadic.
E. coli in Ground Beef
At one time, before the widespread dissemination of E. coli
throughout the food chain, HUS secondary to E. coli O157:H7
infection was known as hamburger disease. The ground beef
c04.indd 70 1/14/2016 11:24:28 AM
CHAPTER 4 • Protein‐Based Foods: Introduction and Red Meats • 71
connection has not gone away. Numerous outbreaks and
massive recalls of contaminated ground beef continue to
plague both the industry and the public.
Meat can become contaminated at any point along the
supply chain and even during preparation. With ground
meat, unlike steak and chops where the contamination stays
on the surface, processing and preparation can result in the
contamination being carried into the center of the meat. It is
recommended that ground beef be cooked to an internal
temperature of 165°F (74°C).
Irradiation offers the most practical and effective way of
sterilizing foods and protecting the consumer. It is already
being used for poultry and ground beef and is approved for
many other foods. Even though the word irradiation conjures
up fears of radiation exposure, irradiated food does not
become radioactive; it is safe, and if properly applied does not
change the taste or texture of food.
Listeria monocytogenes
• Listeria monocytogenes is an extremely serious illness;
although there are only 2500 confirmed cases a year, the
fatality rate is high (25–75 percent) and depends on the
health condition of the person affected. Although healthy
adults and children may consume contaminated foods
without becoming ill, those at increased risk for infection
(especially immuno‐compromised, elderly, or children)
can get listeriosis after eating food contaminated with
even a few bacteria. It is especially dangerous for babies,
both in utero and newborn. Immuno‐compromised per-
sons have the highest listeriosis fatality rates.
• Listeria has the unique ability to enter white blood cells,
thereby avoiding recognition by the immune system, and
from there it can invade the bloodstream and every organ
in the body, including the brain and, in pregnant women,
the fetus. In addition to gastroenteritis, fever, vomiting,
and diarrhea, illness can progress to meningitis, septice-
mia, encephalitis, spontaneous abortion, and death. The
incubation period (time between ingestion and the onset
of symptoms) can be as many as 70 days. This makes iden-
tification and elimination of the contaminated food very
difficult and can lead to many more individuals becoming
infected before the contaminated food is identified,
resulting in many more resulting illnesses and deaths.
• Unique to pathogens, Listeria can grow and multiply
under refrigerated temperatures (37°F [2.78°C]), which
makes this organism of particular concern to food plants
where uncooked, chilled foods (deli salads, deli meats,
sandwiches, etc.) are manufactured.
• Listeria is found in raw or improperly pasteurized milk,
raw milk cheeses, ice cream, raw vegetables, fermented
raw‐meat sausages, raw and cooked poultry, raw meats
(all types), deli meats, and raw and smoked fish. Listeria
is killed by pasteurization and cooking; however, in cer-
tain ready‐to‐eat foods, like hot dogs and cold cuts,
contamination may occur after cooking but before
packaging.
Meat Cookery
Meat is the most expensive part of any meal; extra attention
must be paid to preparing it properly to maximize taste, tex-
ture, juiciness, color, and aroma. Selecting the right cut of
meat for each preparation method is critical to a successful
meal; even the highest‐quality cut of meat can be ruined by
selecting the wrong cooking method. However, the least ten-
der cut of meat can be made flavorful and tender with the
right cooking method.
Choosing the correct cooking method (dry heat/moist
heat) based on knowledge of the different meat cut charac-
teristics, along with appropriate seasoning, timing, and sauc-
ing, is critical to culinary success.
Applying heat to meat not only changes the flavor and
nutritional availability but also can have a big impact on ten-
derness. The two components of meat most responsible for
tenderness, or lack thereof, are muscle fibers and connective
tissue (collagen). With excessive heat, muscle fibers shrink
Source: National Cattlemen’s Beef Association.
FIGURE 4.11 Beef carcass wholesale cuts.
c04.indd 71 1/14/2016 11:24:28 AM
72 • CULINOLOGY®
Source: National Cattlemen’s Beef Association.
FIGURE 4.12 Pork carcass wholesale cuts.
Source: National Cattlemen’s Beef Association.
FIGURE 4.14 Lamb carcass wholesale cuts.
Source: National Cattlemen’s Beef Association.
FIGURE 4.13 Veal carcass wholesale cuts.
and squeeze out moisture, becoming dry and tough. This can
be minimized by low and slow moist‐heat cooking methods,
but then flavor can be compromised. Low heat and high mois-
ture inhibit the chemical processes of caramelization and the
Maillard reaction, both responsible for much of the roasted
and browned flavor development in meat cooked by dry‐heat
methods. On the other hand, collagen, a normally tough and
chewy connective tissue, gelatinizes and softens when sub-
jected to slow heating with high moisture. In general, muscles
with lesser amounts of connective tissue (tender cuts such as
tenderloin, rib eye, etc.) should be cooked via dry‐heat meth-
ods; muscles with higher amounts of connective tissue (tough
c04.indd 72 1/14/2016 11:24:29 AM
CHAPTER 4 • Protein‐Based Foods: Introduction and Red Meats • 73
cuts such as chuck, shoulder, shank, etc.) do better with moist‐
heat cooking methods.
Changes During Cooking
Dry‐heat cooking methods bring about the following flavor
and textural events due to chemical and physical changes:
• Proteins coagulate, meat fibers shorten.
• Fats melt and break down.
• Organic acids, which enhance flavor and texture, are
released.
• Nitrogen‐containing compounds react to form meaty
flavors.
• Water evaporates, causing flavors to concentrate.
• Maillard browning occurs (dry‐heat cooking only), pro-
viding brown, roasted flavors and colors.
Obviously, moist‐heat cooking methods do not provide an
environment that supports most of these changes, so most
flavor generation must take place outside the meat—that is,
by means of flavorful marinades, highly seasoned cooking liq-
uids, and tantalizing sauces.
Meat Processing
Ingredients
As you read ingredient statements, you will find differences
in the types of ingredients as well as the amounts used. These
become even more obvious as you begin to compare similar
products from different regions of the world. Economics play
a major role; in poorer countries, many people cannot afford
to buy products with high levels of protein, so protein is
replaced with larger amounts of carbohydrates, fats, and even
water.
Availability also plays a major role in the types of ingredi-
ents used in the formulation. For example, corn or potato
starch is likely to be used in the United States, while wheat
starch may be the ingredient of choice in Russia.
Food safety is always a concern, with new methods and
ingredients constantly being developed to enhance the safety
of food. Some of these methods affect the proteins, causing a
loss of moisture or a change in texture. Addition of other
ingredients that help retain these components may be
required to preserve the original palatability.
As market size increases and processors expand the areas
where their products are offered, regional regulations, reli-
gious prohibitions, and ethnic preferences may also require
changes in ingredients to fit the prevailing culture. These
changes may affect both texture and flavor. Other ingredients
may be required to increase shelf life or allow meat products
to survive long distance, even international, distribution.
Consumer trends also play a big role. Sodium reduction is
a health trend that requires the replacement of sodium with
other ingredients that perform a similar function, whether it
is for flavor, longer shelf life, or texture modification. Salt is a
basic ingredient for most protein‐based foods; it provides
both functionality and flavor. The salt used for manufactur-
ing is primarily sodium chloride (NaCl), which comes in
many forms. Table salt, the most common type, is finely
ground, while kosher salt is coarser. Because sodium is impli-
cated in high blood pressure and other heath disorders, salt
has been targeted for reduction in many foods. As an alterna-
tive, potassium chloride has been used. Unfortunately, potas-
sium has a bitter, metallic taste when used at high levels, with
some people having greater sensitivity to its flavor than oth-
ers. A rule of thumb is to keep the potassium chloride level at
less than 50 percent of the sodium chloride—but even at that
level some people can detect the off flavor.
Another alternative is sea salt. Today a variety of sea salts
are available from different regions, with different flavors, dif-
ferent mineral makeup (including different levels of sodium),
and different appearances. Sea salt is not all sodium chloride;
it contains varying quantities of potassium chloride, magne-
sium chloride, and other impurities—minerals that impart
different taste sensations, one of which may be enhancement
of flavor. These impurities can also impart bitter off notes
that may be detected by some consumers.
Before the advent of refrigeration, salting meat was a way
to extend shelf life and minimize the growth of both spoilage
and pathogenic organisms. Adding salt essentially decreases
water activity (free water) and creates an environment less
suitable for microbial growth. Reducing the level of salt can
reduce the shelf life of the product, so alternative preserva-
tives may need to be added.
The chlorine in salt aids in the extraction of salt‐soluble
proteins from the muscle, allowing the retention of higher
amounts of water and creating a sticky surface following a
period of mixing or tumbling. This sticky surface gels upon
cooking and acts as glue to hold pieces of meat together. This
methodology is used in the manufacture of sausages, restruc-
tured hams, hot dogs, and other comminuted (minced) or
re-formed meat products. Even though many smaller pieces
of meat are used in the formation of these products, after
heating they can be sliced and remain intact.
The use of alkaline phosphates in commercial marinades
can optimize moisture retention and maximize yield in
meats as well as enhance emulsification and texture. Sodium
tripolyphosphate (STPP) is the one most common in the
meat processing industry. Adding STPP to meat raises the pH
to a more alkaline level, resulting in more negative charges on
the protein molecule, which allows for more moisture to be
trapped. But higher pH in meat may also have detrimental
effects. Meat pigments become more heat stable at a higher
pH. As discussed earlier, this means that the red or pink color
associated with undercooked meat products is retained even
when the product is fully cooked. This can be off‐putting,
especially in poultry.
Phosphates with high pH can also result in off flavors that
are described as metallic or soapy. Moving away from using a
phosphate with a high pH to one that is closer to a pH of 7
can reduce or eliminate some of these detrimental effects. To
achieve a pH of 7, combinations of alkaline phosphates and
acid phosphates have been created. Although there may be
c04.indd 73 1/14/2016 11:24:29 AM
74 • CULINOLOGY®
some reduction of moisture retention as it relates to yield,
modern processing systems help minimize this effect.
Antioxidants are often used in protein foods to protect fla-
vor and shelf life, particularly to prevent those off flavors
(such as warmed‐over flavor) that are the result of the
oxidation of fats. Butylated hydroxyanisole (BHA) and
butylated hydroxytoluene (BHT) are common synthetic anti-
oxidants used together or individually in many food prod-
ucts. Use levels are regulated and cannot exceed 0.01 percent
of the fat (oil) component if used individually or 0.02 percent
of the fat (oil) component if used together. Propyl gallate is
another ingredient commonly used to reduce oxidation.
These antioxidants are not considered natural. Rosemary
extract (carnosic acid and carnosol) has antioxidant proper-
ties and is often substituted for BHA and BHT where a natu-
ral claim is desired. The antioxidant properties of rosemary
can be as effective as BHA and BHT at the same use levels,
but unlike the synthetics, which have no taste, rosemary
extract does add an herbal flavor to the food.
A variety of sweeteners are used in the food industry.
Especially in meat processing, sugars are added to reduce the
harshness of salt. Sucrose, or table sugar, is the most com-
mon and provides the anchor for the sweetness scale, having
a value of 100. Sucrose is often derived from sugar cane or
sugar beets. Being a non‐reducing sugar, it does not promote
Maillard reactions and therefore is considered non‐brown-
ing. Dextrose is another sweetener used in meat prepara-
tions, but it is not as sweet as sucrose, having a sweetness
index of 75. However, being a reducing sugar, dextrose is
involved in Maillard reactions and can cause browning in
food products, which may or may not be an advantage.
Fermentation of meats is a common and inexpensive
way to preserve them while creating a range of sensory
enhancements (flavor, texture, aroma). Salami, pepperoni,
and chorizo are all examples of fermented meats. Meat does
not contain sufficient intrinsic levels of carbohydrate to
support fermentation, so some amount of fermentable
carbohydrate must be added. Dextrose is a simple sugar
(monosaccharide) composed solely of glucose, therefore it
is easily metabolized by microorganisms and is the carbo-
hydrate of choice for fermentation. Corn syrup and corn
syrup solids are also used. Note: These ingredients have a
sweetness and browning potential measured as dextrose
equivalents (D.E.). As the D.E. increases, sweetness and
browning potential increases.
A number of other natural and artificial sweeteners are
available to the food industry, but none have been approved
for use in meat applications.
A number of microbial inhibitors are available; the two
most often seen on the ingredient statement of a cooked
meat product, such as turkey deli meat or bologna, are
sodium lactate and sodium diacetate. These are derivatives
of lactic acid and acetic acid, respectively. Used in combina-
tion, these ingredients have been found most effective against
Listeria monocytogenes in ready‐to‐eat (RTE) meat products.
Their use is required to achieve the highest‐level processing
for RTE meat products as established by the USDA.
Mold growth on meat products is usually seen only on
products with lower water activity that won’t support the
growth of bacteria. These products include salami, jerky, and,
in some cases, hams. For some salamis, the white coating on
the outside is a Penicillium sp. that is desirable and provides
preservative qualities. For other products, mold growth is
detrimental, as it reduces yield and is aesthetically displeas-
ing. Propylparaben and potassium sorbate are two products
that minimize mold growth. They are normally used as a
spray or dip application to the surface of the product to help
prevent mold from growing. Casings on meat products are
sometimes dipped in a solution of one of these mold inhibi-
tors to eliminate growth.
Flavor enhancers are sometimes added to give a more
savory note (known as umami). Monosodium glutamate
(MSG) is one of the more widely used and most controversial
enhancers used in foods. As the name implies, MSG is a
derivative of glutamic acid, one of the amino acid building
blocks of protein. MSG has a binding site on the taste buds
that enhances flavors when it is present. Other sources of glu-
tamates (flavor‐enhancing salts of glutamic acid) include
hydrolyzed vegetable protein (HVP) and autolyzed yeast
extract (AYE). Hydrolysis and autolysis break down a protein
into its amino‐acid building blocks, releasing glutamic acid
and providing a taste response similar to MSG without hav-
ing to put MSG on the ingredient label.
Ribotides such as inosinate and guanylate (I + G) are also
highly effective flavor modifiers in many foods. However, they
are phosphorylated molecules and consequently susceptible
to phosphatase enzyme activity. Food ingredients that pos-
sess phosphatase activity, such as raw meats or untreated
wheat flour, must be heat‐treated to inactivate these enzymes
before ribotide is added to the product.
By definition, products with added sodium nitrate or
sodium nitrite are considered cured. Nitrites, when com-
bined with meat pigments, result in the characteristic pink
color consumers expect in hams, frankfurters, bacon, and
other cured products. (In the United States, nitrate is not
allowed in cured meat products other than dried sausages.)
In addition to color fixing, nitrite also provides antioxidant
activity, imparts some of the flavor associated with cured
meat products, and has antimicrobial activity, in particular
against Clostridium botulinum. The latter is extremely impor-
tant in light of the controversy surrounding the use of nitrite.
Under certain conditions not yet fully understood, the
natural breakdown products of proteins, known as amines,
can combine with nitrites to form compounds known as
nitrosamines. There are many different types of nitrosamines,
most of which are known carcinogens in test animals. Many
variables influence nitrosamine levels: amount of nitrite
added during processing, concentrations of amines in meat,
types and amounts of other ingredients used in proc essing,
actual processing conditions, length of storage, storage tem-
peratures, method of cooking, and degree of doneness. For
example, well‐done bacon is potentially more hazardous
than less well‐done bacon. Bacon cooked by microwave has
less nitrosamine than fried bacon. Drippings usually contain
c04.indd 74 1/14/2016 11:24:29 AM
CHAPTER 4 • Protein‐Based Foods: Introduction and Red Meats • 75
more nitrosamines than the cooked bacon. That said, no link
has been proven between eating bacon or other cured meats
and cancer in humans, only in laboratory animals.
Nitrite is the only known compound added at 200 ppm or
less that has the ability to restrict the growth of Clostridium
botulinum. This is a major reason why it has not been removed
from cured meat products. Clostridia are anaerobic microor-
ganisms. Cured meats are normally vacuum packed. If this
organism were inadvertently introduced to the interior of the
meat during processing, the oxygen‐less environment would
encourage its growth. Although current sanitation proce-
dures virtually eliminate this possibility, nitrite is a tool that
guards against its occurrence. In addition, consumers also
look for the characteristic color associated with cured meats.
Without nitrite, these meats (bacon, ham, sausage, etc.)
would look gray rather than pink. In fact, in some parts of the
country, corned beef is available in both standard cure (pink)
as well as “gray cure” for consumers accustomed to (and pre-
ferring) that appearance.
The processes followed for curing meat products have
changed throughout the years, with processing times getting
shorter due to improved technology. Where hams were once
brined for up to 10 days before cooking, they are now injected,
tumbled, and cooked within 24 hours. Processors discovered,
however, that the color developed with these shorter proc-
essing methods is not as stable as with the longer curing
times. It was found that reductants (antioxidants), in the
form of sodium erythorbate or sodium ascorbate, could be
added to commercial meat cures to promote more stable
color formation. These reductants assist in the transforma-
tion of nitrite to nitric oxide, which is the form that reacts
with the meat pigment for color formation. As a bonus, use of
these compounds not only allows for less nitrite to be added
but also inhibits the formation of nitrosamines. In fact, in
commercially mass‐produced (“pumped”) bacon, addition of
ascorbate or erythorbate is required by the USDA.
For the manufacture of dry products such as salami and
pepperoni, the goal is to reduce the amount of moisture in
the meat to enhance shelf stability, texture, and flavor.
Reducing pH assists in accomplishing that goal by decreasing
the protein’s ability to bind water. However, adding acid
directly to proteins hydrolyzes the protein and destroys meat
texture. Instead, using fermentative lactic acid bacteria to
form lactic acid in the meat more gradually allows for dehy-
dration without degrading the texture. Today, modern proc-
essors purchase specific bacterial starter cultures selected for
their ability to grow and produce acid in meat mixtures under
established conditions. Dextrose must always be added, as
not enough carbohydrate is normally present in meat to sup-
port fermentation.
To enhance texture or improve the product’s ability to
retain moisture, some processors add starches and gums,
collectively referred to as binders, to meat. Starches come
from a variety of sources, most often corn, potato, or rice.
Rice starch is usually the starch of choice because it has the
smallest granules and can squeeze into tight spaces in the
muscle more easily than potato or corn can.
Carrageenan is a hydrocolloid gum derived from seaweed;
it is used by meat processors to help retain moisture, firm
texture, and improve slicing. Having characteristics similar to
starch, carrageenan swells and eventually solubilizes when
heated and then, depending on the type used, can form a gel
upon cooling. The specific characteristics of the gel are
dependent on the type of carrageenan used. Kappa carra-
geenan forms a very firm but brittle gel, whereas iota carra-
geenan is less firm and more elastic. Lambda carrageenan
does not gel. Kappa and iota carrageenans are most often
used in meat processing where gelling helps lock moisture in
the meat, while lambda carrageenan is used in sauces and
dressings where gelling is not desired.
Not all of the meat harvested from an animal can be cut
into roasts, steaks, or chops. The trimmings are commonly
used for the manufacture of ground meat products. Re –
structured meat, also known as chopped and formed meat (as
opposed to whole muscle), uses meat trimmings and binding
agents (such as egg albumin, gelatin, soy or milk proteins, or
enzymes) to produce re‐formed meat items that can be very
similar in appearance, taste, and texture to the real thing. The
ability to manufacture these items with uniform shape and
consistent weight makes them ideal for foodservice opera-
tions to eliminate waste and maximize profit.
Alginate is another carbohydrate extracted from seaweed.
It has the unique ability to gel in the presence of calcium and
thus can be used to bind together smaller pieces of meat.
Combining the meat chunks with alginate and a calcium
source and pressing them into a mold yields a product with
the characteristics and appearance of whole muscle.
Transglutaminase is an enzyme that promotes binding
between proteins and assists in the formation of a whole
muscle–type product from meat trimmings.
Fibrimex® is a system composed of thrombin and fibrino-
gen. These products are normal components of blood and are
responsible for the clotting mechanism of blood when the
body is cut or injured. This clotting mechanism has been
adapted to meat products to help hold pieces together for a
whole muscle characteristic. One of the primary uses of
Fibrimex® is binding two whole beef tenderloins butt to tail
to achieve a higher yield of tenderloin steaks. Using current
market conditions, this process could improve the return on
100 pounds of tenderloins by $300. Note: These ingredients
work equally well with any protein source: meat, poultry, or
seafood. A word of caution: Any restructured meat product
may allow pathogenic organisms that were on the outside
surface to wind up on the internal portion of the meat. The
resulting product should be cooked to an internal tempera-
ture sufficient to kill those microorganisms.
Conclusion
An understanding of protein chemistry is essential for the
practicing Culinology® professional. Regardless of the source—
red meat, poultry, seafood, or vegetable—protein is an essen-
tial component of the human diet; we cannot live without it!
As such, it is very likely that at some point in your career you
c04.indd 75 1/14/2016 11:24:29 AM
76 • CULINOLOGY®
will be involved in developing protein‐based foods. The
information presented in this and the following three chap-
ters provides a solid overview of this subject.
1 K. Imura and A. Okada, “Amino Acid Metabolism in Pediatric Patients.”
Nutrition 14:1(1998): 143–148.
2 G. Sarwar and F. E. McDonough, “Evalution of Protein Digestibility:
Corrected Amino Acid Score Method for Assessing Protein Quality of
Foods.” Journal of the Association of Analytical Chemists 731(1990):
347–356.
3 M. C. Hunt, O. Sorheim, and E. Slinde, “Color and Heat Denaturation of
Myoglobin Forms in Ground Beef.” Journal of Food Science 64(1999):
847–851.
4 Center for Disease Control. www.cdc.gov/ncidod/dvrd/prions/ Accessed
March 17, 2011.
5 Center for Disease Control. www.cdc.gov/ncidod/dvrd/bse Accessed
March 17, 2011.
6 Creutzfeldt-Jakob Disease International Surveillance Network www
.eurocjd.ed.ac.uk/surveillance%20data%201.html#vcjd-cases Accessed
November 15, 2015.
7 E. D. Belay and L. B. Schonberger, Annual Review Public Health 26(2005):
191–212.
8 E. Scallan, R. M. Hoekstra, F. J. Angulo, R. V. Tauxe, M. A. Widdowson, S. L.
Roy, J. L. Jones, P. M. Griffin, “Foodborne Illness Acquired in the United
States—Major Pathogens.” Emerging Infectious Diseases 17:1(2011): 7–15.
9 Explore Beef. www.explorebeef.org/cmdocs/explorebeef/factsheet_
antibioticuseincattleproduction Accessed November 15, 2015.
10 Centers for Disease Control and Prevention. http://www.cdc.gov.
11 C. W. Ang, M. A. De Klerk, H. P. Endtz, B. C. Jacobs, J. D. Laman, F. G. van der
Meche, P. A. van Doorn. “Guillain-Barre Syndrome and Miller Fisher
Syndrome-associated Campylobacter jejuni Lipopolysaccharides Induce
anti-GM1 and anti-GQ1b Antibodies in Rabbits.” Infection and Immunity
Apr;69(2001)(4): 2462–2469.
12 R. V. Tauxe, “Epidemiology of Campylobacter jejuni Infections in the United
States and Other Industrial Nations.” In: I. Nachamkin, M. J. Blaser, and L. S.
Tompkins (eds.). Campylobacter jejuni: Current and Future Trends
(Washington: American Society for Microbiology, 1992, pp. 9–12).
13 P. S. Mead, L. Slutsker, V. Dietz, L. F. McCraig, J. S. Bresee, C. Shapiro, P. M.
Griffin, and R. V. Tauxe, “Food Related Illness and Death in the United
States.” Emerging Infectious Diseases 1999 Sep-Oct; 5(5): 607–625..
c04.indd 76 1/14/2016 11:24:29 AM
CHAPTER 5 • Protein‐Based Foods: Poultry • 77
Introduction
The United States may be the land of the hamburger, but
Americans eat more poultry than any other protein.
According to the American Meat Institute, poultry consump-
tion continues to grow and remains a large percentage of the
food market—some 48.2 percent of all meat consumed in the
United States. 1
In 2012, U.S. poultry production totaled 43.5 billion
pounds. 2 Of that, chicken accounted for 37.6 billion pounds,
and turkey totaled nearly 6 billion pounds. 3
Over the past several decades, poultry production has
become highly vertically integrated from production to
harvest. This vertical integration has allowed the industry to
streamline and reduce production costs, making poultry a
very affordable food for the consumer. Advancements are
enormous in the areas of raising, harvesting, fabrication, pack-
aging, and transportation. In addition, the poultry industry
continues to lead the way in new products and innovation.
In the last few decades, the marketing of poultry has
increasingly changed from whole birds to bone‐in parts to
skinless and boneless products. Consumers now focus on
convenience and health, and as their needs have changed, so
has the way poultry is processed and marketed. In addition,
the industry continues to find ways to add value to lower‐
cost cuts, such as thigh and leg meat.
The processing of live birds remains similar to the past, though
novel approaches to improve food safety continue to emerge.
Improvements in handling and animal welfare, along with key
improvements in processing efficiency and yield enhancement,
are a major priority for the industry as profit margins shrink
due to rising feed, transportation, and proc essing costs.
Pre‐Harvest Handling
It is important to handle poultry, like other livestock, care-
fully to avoid bruises, abrasions, and broken limbs. Typically,
poultry used for meat production are raised on wood shavings
in large commercial houses that contain approximately
25,000 broilers (chickens under 13 weeks old, which consti-
tute virtually all commercial chicken production) or 13,000
turkeys. Through advancements in poultry genetics and feed
formulations, commercial broilers are typically ready for proc-
essing 6 to 7 weeks from hatch, turkeys in 15 to 20 weeks.
Before catching, loading, and transporting commercial
poultry to the processing plant, feed is removed from the
birds for 8 to 12 hours in order to help empty their intestinal
contents. This process is commonly known as feed with-
drawal . During feed withdrawal, the birds are allowed unre-
stricted access to water until they are caught and loaded for
processing. Once the birds arrive at the processing plant,
they are processed according to standard ethical processing
procedures. If birds are not allowed adequate time for feed
withdrawal at the time of processing, there is an increased
risk of intestinal breakage during slaughter, leading to fecal
contamination inside or on the surface of the bird. The
USDA has a zero tolerance for visible fecal contamination,
so any carcasses that are contaminated must then be segre-
gated, thoroughly cleaned, and re‐inspected before entering
the chiller.
Most poultry is loaded into catch modules or coops for
transportation by either manned mechanical loaders or
catch‐crews. During the loading and unloading process, spe-
cial care must be taken in order to minimize any additional
stress or injuries to the birds. Some of the most common inju-
ries include broken wings, lower leg injuries, bruises, and
scratches, all of which can potentially lead to carcass down-
grades during processing and compromise ethical treatment.
Once the birds reach the plant, the modules or coops are
removed from the truck to a conveyor belt where the birds
are disgorged. The birds are then transported by conveyor
belt into the plant, and enter the hanging room where they
are hung upside down on shackles before they are stunned.
Often the hanging room is lit only with ultraviolet lights in
order to calm the birds and minimize additional stress during
this process.
Lead Author: L. Cain Cavitt, Ph.D., CCS® , Project Leader, Research & Development , Tyson Foods, Inc.
Contributor: Jason M. Behrends, Ph.D., CCS®, Project Leader, Tyson Foods, Inc.
5
Protein‐Based
Foods: Poultry
c05.indd 77 1/22/2016 1:00:18 PM
78 • CULINOLOGY®
Stunning
The majority of all commercially processed birds are stunned
(by either electricity or modified atmosphere gases) before
slaughter. In addition to immobilizing the birds, electrical
stunning helps facilitate the removal of their feathers by para-
lyzing the muscles in which their feather follicles are embed-
ded. Typically, stunning is done by electrical shock in a
charged 1% salt‐water bath. Salt water is used because it is a
better conductor of electricity than water alone. This type of
stun is called a “reversible stun,” which means that the birds
are not actually killed during the stunning process. The stun
only renders the birds unconscious for 1–2 minutes and, if
left alone, they will completely recover within about 5 min-
utes. A reversible stun allows for the birds’ heart rate to slow
down and become more uniform before slaughter. A tradi-
tional electric stun is applied for approximately 10–12 sec-
onds at 10–20 mA per chicken and 20–40 mA for turkeys.4
With electrical stunning, certain processing issues can
occur, resulting in potential carcass downgrades. Oftentimes
if the amperage is too high the result can lead to broken
bones in the wing and clavicle due to an involuntary super‐
contraction of the skeletal muscles. It can also result in pete-
chial hemorrhaging of residual blood in the capillaries of the
breast muscle, resulting in small visible blood spots just
under the surface of the muscle membrane. If too low an
amperage is used, it is possible that the birds will not be ren-
dered unconscious and could be miscut entering the kill
machine. This might result in poor bleed‐out, or in increased
broken wing bones due to excess flapping as the disoriented
chicken passes through the stunning cabinet and kill
machine. Of course, a primary objective of stunning is to
eliminate as much as possible any suffering that the animal
might experience as it continues through the slaughter
process.
Alternative methods have been tested including modified
atmosphere stunning, which has gained popularity in Europe
and is beginning to be used here. Modified atmosphere stun-
ning typically either uses a mixture of carbon dioxide (10–
40%) and air (60–90%) or carbon dioxide (30%), argon
(55–70%), and nitrogen (0–15%).5 Both of these methods ren-
der the birds near death before bleed‐out, which reduces the
frequency of broken bones and blood spotting. This type of
stunning is referred to as an “irreversible stun,” which means
that the birds cannot recover from the stunning process.
Note: Certain religious food rituals, such as halal (Islamic)
or kosher ( Jewish), do not allow stunning before processing:
They feel that the stunning process itself is inhumane.
Instead, the birds are blessed by an Islamic imam or Jewish
rabbi before entering the kill step and are then quickly dis-
patched by a cleric trained in ritual slaughter.
Killing and Bleeding
Immediately after stunning, the birds enter the kill machine
where a rotating circular blade severs the carotid artery and
jugular vein, allowing for a quick and uniform bleed‐out.
Potential carcass downgrades can result if the blade cuts too
deeply, thereby cutting the esophagus and spinal cord. If the
spinal cord or head is completely severed, the carcass can
begin to twitch and jerk uncontrollably, leading to broken
wings and blood splash in the breast muscle due to involun-
tary muscular contractions. On the other hand, if the cut is
too shallow, the result could be a poor bleed‐out, which could
discolor the muscle tissue because of elevated amounts of
blood remaining in the bird when entering the scalding
phase. Normally birds are allowed to bleed‐out for a period of
90–120 seconds before entering the scalder.
Scalding and Feather
Removal
Scalding entails submerging the carcass in hot water to
loosen the feathers from the follicles. There are two types of
scalding methods most commonly used: hard and soft scald-
ing. Hard or hot scalding water (145–148°F) for 45 seconds is
a method that removes the “waxy” pigmented cuticle layer
(outer, epidermal layer) from the skin, leaving it with a
washed‐out white appearance.6 Removing the waxy cuticle
allows for better batter‐breading adhesion when making
fried products.
The temperature for soft‐scalding ranges from 125–132°F
for 20–30 seconds for broilers, and up to 60 seconds for older
birds.7 Soft scalding, due to the lower water temperature,
does not remove the pigmented waxy cuticle layer of the skin.
A soft scald is most often employed when the processor
wants to keep the yellow skin color. This type of appearance
is highly desired in certain parts of the United States and
other countries. Because the cuticle has not been removed in
soft‐scalded birds, trying to batter‐bread and fry them is
more difficult as the breading doesn’t properly adhere to the
skin.
After scalding, all birds then pass through the feather
picker. Most commercial feather pickers consist of a series of
rotating rubber fingers that gently remove the feathers from
the birds as they pass through picker tunnels that are pre‐set
for each size of bird. If the rotating fingers are set too close to
the bird, skin tears and broken bones may result. Alternatively,
if the rotating rubber fingers are set too far away from the
birds, the result could be a poorly picked bird with numerous
feathers left on the carcass.
Evisceration
Evisceration is the process in which the viscera—internal
organs, both edible and inedible—are removed from the car-
cass. Edible viscera (the “giblets”) include the liver, heart, and
gizzard while the inedible viscera include the crop, intes-
tines, kidneys, lungs, and reproductive tract. In the past, this
part of the process tended to be the most labor‐intensive as
it was performed by hand. Today, in most commercial proc-
essing plants, this phase of processing has become almost
completely automated. The visceral package (intestines,
c05.indd 78 1/14/2016 7:18:26 AM
CHAPTER 5 • Protein‐Based Foods: Poultry • 79
heart, lungs, liver, gizzard, and reproductive organs) is
removed from the carcass, presented to USDA inspectors,
and then separated into edible and inedible components.
The edible viscera is commonly packaged and placed in the
cavity of carcasses being sold “whole.” Additionally, the neck
is often (especially in turkeys) included in the edible viscera.
In addition to the edible viscera, the feet (commonly referred
to as “paws”) are harvested shortly after evisceration,
cleaned, and sold to countries that use them for human
consumption.
Chilling and Carcass
Aging
As with all livestock carcasses, it is important to remove body
heat as quickly as possible to reduce microbial spoilage and
potential pathogen growth. U.S. regulations require that all
commercially processed poultry reach 39.2°F (4°C) or less
within 4 hours of death for chickens and 8 hours for turkeys.8
Two types of commercially available chilling methods are
normally used today: immersion (water chilling) and air chill-
ing. Immersion is most commonly used by the commercial
broiler industry in the United States.
Immersion chilling consists of a two‐step system: the pre‐
chill stage followed by the chill stage. In the first step, fresh
carcasses enter a large tank filled with cold water (45–55°F
[7.2–10°C]) and are pushed along by either a screw‐type
auger or large paddles. The carcasses remain here for approx-
imately 15 minutes and then transition to the chill stage, dur-
ing which they remain in 33–35°F (0.6–1.7°C) water for 45–55
minutes.
As the carcasses enter the chiller, they typically have an
internal body temperature of 100.4°F (38°C). By gradually
reducing the temperature of the water from 55°F (10°C) to
33°F (0.6°C) over the course of the chilling time, the core body
temperature of the bird is slowly reduced. A warm carcass
immediately subjected to the colder chiller water has an
increased chance of undergoing cold shortening. Cold short-
ening is a quality issue that dramatically reduces the amount
of tender meat by causing a supercontraction of the actin and
myosin filaments of muscle fibers, which become locked in
place due to the sudden exposure to extreme cold. The out-
come is a carcass or parts that are both extremely tough and
have reduced water absorption and holding capacity during
marination.
Air chilling is more commonly used in Europe. It requires
substantially more room than immersion chilling (air is a less
efficient temperature transfer medium than water). The fresh
carcasses pass through a large room where temperature and
wind velocity are controlled; the cold air flow reduces the
core body temperature of the bird over 1 to 2 hours. Upon
completion of this stage, air‐chilled carcasses are at the same
temperature as those that pass through the immersion chill-
ing process. The major difference between the two processes
is that the immersion chilling system allows for some water
uptake in the carcass, thereby slightly increasing yield, while
birds sent through the air‐chilling system typically dry out
more and have a greater yield loss.
Carcasses exiting the chiller (approximately 2 hours post-
mortem) often are cut in half around the middle. The back
half is typically sold as leg quarters, whole legs, or drumsticks
and thighs rather than boneless items, so no additional aging
is required. The front half contains the breast fillet, tender-
loin, and wings attached to the back and rib cage. (The so‐
called “football” contains just the breast fillet and tenderloin
still attached to the back and rib cage.) Because a large major-
ity of products use either the boneless breast fillet or tender-
loin, it is essential that these two sets of muscles, left on the
bone, be aged on ice or in a cooler 6 to 8 hours before harvest-
ing in order to ensure a tender product.
Rigor mortis (Latin for “stiffness of death”) is classified as
one of the most dramatic postmortem changes that occur
during the conversion of muscle to meat. The rate of rigor
mortis development and resolution varies with the species
and body size. It typically takes 6–8 hours for commercial
broilers and 8–10 hours for commercial turkeys to complete
the rigor process. Harvesting or deboning breast fillets or ten-
derloins before the completion of rigor mortis results in a
toughening of the meat due to residual energy as adenosine
triphosphate (ATP) is still present within the muscle cell. If
too much ATP is left in the muscle at deboning, the muscle
fibers contract, creating a denser piece of meat that in turn is
tough when cooked. Tenderness is one of the most important
factors in overall consumer acceptability of broiler breast,9
and commercial processors take special care when harvest-
ing breast meat and tenderloins to ensure it.
Dressing Percentage
The breed and species of poultry play a huge role in the value
and variety of meat produced. Poultry varieties may be thinly
muscled to heavily muscled, and many species are used for
food, including chicken, turkey, ostrich, duck, and so on.
These factors dramatically change the dressing percentage
(amount of salable carcass after harvest and removal of head,
feet, feathers, blood, and viscera) that can be achieved. For
the most part, the chicken and turkey industries produce
birds that are thick and heavily muscled to optimize meat
production; these have an average dressing percentage of
70–80 percent.
Breed, species, ability to convert feed to muscle efficiently
(feed is 70 percent of the production cost of raising poultry),
and condition of the birds all affect dressing percentage. For
example, chickens weighing less than 5 pounds versus those
weighing more than 5 pounds can lose an average of 11 per-
cent versus 9 percent, respectively, in blood and feathers, and
when completely dressed can lose a total of 27 percent versus
25 percent, respectively.10 These differences give the industry
an economic incentive to produce chickens over 5 pounds to
reduce cost per pound.
Whichever breed, species, or size of bird the industry tar-
gets, the goal is to improve dressing yields and lower the cost
per pound of meat produced.
c05.indd 79 1/14/2016 7:18:26 AM
80 • CULINOLOGY®
Poultry Types
The Meat Buyer’s Guide, published by the North American
Meat Institute (formerly NAMP, the North American Meat
Processors Association), is a recognized reference for cutting
and grading meat. The Guide provides a standard numbering
system for all classes and cuts of meat and poultry sold com-
mercially, and assigns each one a code number. For poultry,
the code numbers all contain the prefix “P” followed by a
four‐digit number.
Here are the sizes of chicken along with their NAMP codes,
from largest to smallest:
• Roasters (P1100; NAMP, 2007) are typically 12–16 weeks
old and weigh 3.5–5 pounds once processed.
• Broiler/fryers (P1000; NAMP, 2007) are typically proc-
essed at 6–8 weeks and weigh 3.5 pounds or less once
processed.
• Rock Cornish game hens or Cornish game hens are
young, immature birds (P1500; NAMP, 2007) typically
processed at 4–5 weeks. They weigh 2 pounds or less
once processed.
• Poussin (P1400; NAMP, 2007) are very small, immature
birds, typically processed at 3–4 weeks and weighing
approximately 1–1.5 pounds once processed.
Roasters are similar in size to capons (P1200; NAMP,
2007), which are castrated males that typically weigh
5 pounds or greater at processing. Fowl (P1300; NAMP, 2007),
known as stewing or baking hens, are generally egg layers
processed for meat once they are no longer able to regularly
produce eggs (spent hens). Spent hens or fowl are processed
between 10 and 12 months of age and generally weigh
2.5 pounds or greater once processed. Meat from fowl is gen-
erally tougher than meat from other chickens, so it is used in
products such as diced meat for soups. Additionally, fowl
meat is often exported to Canada, Mexico, and other
countries.
The only breed of turkey raised commercially in the United
States is the broad‐breasted white. Turkeys are available as
hens (P2201; NAMP, 2007) or toms (P2101; NAMP, 2007) and
in sizes ranging from 9 to 30 pounds. They are typically proc-
essed between 16 and 18 weeks of age. Turkeys are most often
sold whole (with giblets) during the Thanksgiving and
Christmas season. Parts that are further processed, such as
drumsticks, boneless, skinless breast, and ground turkey have
gained popularity due to the lean nature of turkey meat. Fully
cooked refrigerated and frozen turkey entrées are also popu-
lar. The primary uses for turkey meat throughout the year are
as deli meat, hot dogs, and fresh ground.
Ducks are classified as duckling (broiler/fryer), roaster
duckling, and mature duck (P3000; NAMP, 2007). The most
common farm‐raised breeds are the Pekin and the Rouen.
Geese are classified as young goose or mature goose (P4010;
NAMP, 2007). The most common farm‐raised breeds are the
Emden and the Toulouse. Game birds now routinely farmed
and commonly available include guinea fowl, pheasant, quail,
common or gray partridge, and Chukar partridge. Ostrich is
also readily available.
Basic Cuts of Poultry
Commercially processed poultry is generally sold raw as
whole birds or cut‐up parts, or processed into boneless, skin-
less meat. A cut‐up bird consists of whole leg (drumstick and
thigh), split breast with tenderloin, wing with tip, wing flat
(mid‐joint), and drummette. Whole birds are sold with or
without giblets (“WOG”). The usual arrangement of cut birds
is called an eight‐piece cut, including two breast halves with
the ribs and back portion attached, two wings, two thighs
with back portion and tail attached, and two drumsticks. The
most common cuts of boneless, skinless poultry items sold
raw are breast, tenderloins, and thighs. These cuts are often
sold marinated as well. Marinated items can include just
about any flavor profile, from basic saltwater brine to a highly
seasoned and complex flavor such as a teriyaki or buffalo.
Today, boneless, skinless processed poultry has the highest
sales volume due to its unmatched convenience.
Poultry Meat
Functionality
The functionality of poultry meat refers to any inherent phys-
icochemical and/or rheological property intrinsic to poultry
protein that influences final product quality. (Note: Many of
the textural properties human beings perceive when they
consume foods are largely rheological in nature—for ex –
ample, creaminess, juiciness, smoothness, brittleness, ten-
derness, hardness.) The three most important functional
properties for the poultry industry are gelation, emulsifica-
tion, and water binding/holding.
Gelation is the process whereby a protein unfolds, fol-
lowed by orderly cross‐linking, to form viscoelastic (gel‐like)
three‐dimensional networks or matrices. Gelation provides
Source: YinYang/Getty Images, Inc.
FIGURE 5.1 Cut pieces of poultry.
c05.indd 80 1/14/2016 7:18:39 AM
CHAPTER 5 • Protein‐Based Foods: Poultry • 81
structure and texture to such products as hot dogs, meat
binding in restructured meats, fat stabilization, and water
holding. Myofibrillar proteins, the salt‐soluble proteins,
provide the most gelation. These include actin, myosin,
and actomysosin, the proteins responsible for muscular
contraction and relaxation—and therefore the texture of proc –
essed meat products.
Meat emulsification is normally achieved by homogeniz-
ing a mixture of meat (protein) and fat, using pressure or high
shear energy to disperse the fat (the discontinuous phase) as
droplets evenly throughout the protein (the continuous
phase). A typical meat emulsion consists of fat globules (usu-
ally ground poultry skin) coated with salt‐soluble proteins
and imbedded in a protein matrix. Examples include bologna
and hot dogs. The coating of salt‐soluble proteins around the
tiny fat globules lowers the surface tension of the fat droplets,
preventing them from coalescing (reuniting) and thus sta-
bilizing the emulsion system.
Factors affecting emulsion stability include the type and
amount of protein available to form the film coating for the
fat droplets as well as processing parameters such as chop-
ping time, meat mixture temperature, and fat‐to‐protein
ratios. These depend on the type of product being produced.
If the product contains too much fat or is overworked, the
meat matrix can collapse and cause the emulsion to break
down, leading to a quality defect known as fatting out or fat
caps. This occurs during the cooking process when fat glob-
ules expand while the proteins coagulate and shrink slightly,
thus rupturing the protein matrix and allowing the fat to rise
to the surface of the product.11
Traditionally, the meat used for emulsified products is
made in part with ground breast meat or mechanically sepa-
rated chicken or turkey (MSC or MST). MSC or MST is gener-
ated by taking trimmed carcass parts such as frames (denuded
carcasses) and backs and separating the remaining scraps of
meat from the skeletal structure by grinding the starting
material and then passing it under high pressure through a
sieve, which removes bones and cartilage. Once the mechani-
cally separated meat is harvested from the frames, it is ground
or emulsified, then bulk packed and shipped out either refrig-
erated or frozen. This inexpensive product is used as filler for
hot dogs and other emulsified products.
Water‐holding capacity is also an important factor in poul-
try processing. Hydrating poultry products can improve or
sustain palatability, tenderness, and juiciness. Product yield
may also be improved, which has economic significance.
Typical methods used to increase water binding include the
use of sodium chloride (NaCl) and phosphates, both of which
help attract and bind water molecules to protein. Phosphates
help dissociate actomyosin and weaken electrostatic interac-
tions between peptide chains, reducing the muscle’s resist-
ance to water absorption. NaCl shifts the protein’s isoelectric
point to a lower pH. The result is the swelling of muscle fibers,
which increases hydration or water‐holding capacity. The net
result is improved yield and better eating quality (texture,
mouthfeel, moistness, tenderness, flavor release).
Further‐Processed or
Value‐Added Poultry
Through advancements in technology, the growing influence
of Culinology® professionals, and the expanding taste buds of
the American consumer, the poultry industry is a leader in the
muscle foods area in developing value‐added products. This
can be seen in the multitude of value‐added poultry products
in the market, including marinated raw or frozen chicken,
fully cooked grilled strips, fully cooked and ready‐to‐eat
breaded tenders and strips, pizza toppings, deli meats, turkey
bacon, popcorn chicken, and pre‐seasoned products of all
types with flavors from every corner of the globe. Under-
standing technical capabilities, market demands, and econo m-
ics is important in developing new products. Improved
technical capabilities have led to new technologies in shaping
and forming products (“chopped and formed” products),
emulsifying, marinating (injection and vacuum tumbling),
coating, a multitude of cooking and smoking methods, chill-
ing and freezing systems, and packaging.
Poultry is one of the most adaptable of meat products and
provides a blank canvas for professionals in research and
development. Poultry meat can be converted to fit just about
any flavor profile and has been the source of much advance-
ment in new protein products over the past several years.
The basic steps to producing further‐processed poultry
include piece‐size reduction or portioning, formulation/
ingredient incorporation, forming, and cooking.
Portioning
Controlling piece or portion size of the raw starting material
is a critical key to developing a new product. Over the last
several years, the majority of broiler processors are moving to
larger birds (from about 6 pounds to 8 pounds) in order to
maximize carcass and processing yields. With the increase
in weight comes an increase in muscle size. Portioning
equipment has been developed that can cut horizontally or
Source: Barcin/Getty Images, Inc.
FIGURE 5.2 Grilled chicken breast.
c05.indd 81 1/14/2016 7:19:10 AM
82 • CULINOLOGY®
vertically, slice, or dice boneless, skinless breast meat to fit
any portion size desired by the customer. In addition to hori-
zontal and vertical slicers, other common types of portioning
equipment include high‐pressure water‐knives, industrial
patty‐formers, and portioners that can turn out millions of
identically shaped and sized breast fillets and patties for fast
food restaurants. Portion control in foodservice is critical to
maintaining profitability.
Ingredient Functionality and
Flavor Enhancement
Today a large percentage of all further processed poultry
products is marinated. Cooked poultry meat, especially white
meat, is inherently bland and dry; therefore, it is often mari-
nated to enhance its overall palatability. A typical marinade
consists of water, salt, sodium phosphates, and other season-
ings and is applied at 10–20 percent of the raw product
weight. Each of the ingredients in a marinade has a specific
function. Salt (sodium chloride; NaCl) is the most common
ingredient used to season any type of food product. In addi-
tion to flavor enhancement, salt causes protein extraction,
which allows for the marinade to be absorbed into the
product. Typical salt usage in poultry products ranges from
0.25–1.0 percent.
Phosphates are traditionally used in conjunction with salt
to help increase the overall moisture‐binding ability of the
meat muscle, which enhances the juiciness, tenderness, fla-
vor, and palatability of the product. Sodium phosphates, such
as sodium tripolyphosphate (STPP), are the type most com-
monly used in meat applications. Phosphate usage is regu-
lated by the U.S. Department of Agriculture (USDA) and is
allowed up to 0.50 percent in the finished product.
Other functional ingredients such as starches, gums, and
soy proteins are often added to commercial marinades as a
means of retaining moisture, both from the marinade and
from free moisture within the muscle tissue. In addition,
these functional ingredients aid in the enhancement of over-
all juiciness and flavor impact of the marinated product.
Starches generally come in two types: native or modified,
and are derived from corn, potato, tapioca, or rice. They are
used at a concentration of 0.75–1.25 percent in the finished
product. Starches are further broken down into two sub-
groups: cold swelling and hot swelling, depending on their
ability to hydrate and absorb moisture at different tempera-
tures. Cold swelling, pregelatinized starches are used in items
that will not be exposed to extensive heating, while hot swell-
ing, cook‐up starches are commonly used in items that will
be fully cooked. At lower use levels, starches are neutral in
flavor impact, but at higher levels, they can leave an off‐white
starchy appearance on the product.
Gums such as xanthan and carrageenan are also used
as a means of retaining moisture and enhancing juiciness.
Xanthan is produced through bacterial fermentation, while
carrageenan is derived from a specific seaweed family. Gums
traditionally have very little to no flavor impact on the fin-
ished product and are used at 0.1–0.3 percent in the finished
product. They are often used to suspend ingredients in mari-
nades that have a lot of visible solids. Like starches, gums
thicken or gel at different strengths and temperatures de –
pending on their functionality and use. (See Chapter 8 for a
more complete discussion on gums and starches.)
Soy proteins are another set of functional ingredients
often used in commercial marinades to enhance juiciness
and flavor development. Unlike starches and gums, soy pro-
teins often impart a slight beany or soy note when used in
savory commercial marinades, and they must be declared as
a soy allergen on the ingredient statement. Soy proteins are
broken down into three groups: soy flour (50 percent pro-
tein), soy protein concentrate (SPC) (70 percent protein), and
soy protein isolate (SPI) (90 percent protein) and are typically
used at 0.75–1.25 percent in the finished product. Like gums,
soy proteins immediately begin to hydrate in the presence of
water, whereas cook‐up starches don’t begin to hydrate until
a certain temperature is achieved. Soy flour and soy protein
concentrate are most often used in marinades applied to
whole‐muscle items; soy protein isolates are most often used
in chopped and formed or emulsified products due to their
ability to bind together lipids and proteins.
Whey proteins (derived from milk) are another functional
ingredient used in further processed meats such as hot dogs
and bologna. They must be declared as a dairy allergen on the
ingredient statement.
Sodium Reduction
Over the past several years, responding to pressure from con-
sumers, the medical community, government, and the media,
the poultry industry has pushed to reduce overall sodium
content in further-processed meat products. Salt tradition-
ally is used as a means of extracting protein and enhancing
overall flavor. However, it contains approximately 39 percent
sodium, which is a major contributor to overall sodium con-
tent. Salt substitutes such as potassium chloride (KCl) are
gaining popularity as a way to reduce overall sodium content
while maintaining similar functionality. Potassium chloride Source: Robert Linton/Getty Images, Inc.
FIGURE 5.3 Whole roasted chicken.
c05.indd 82 1/14/2016 7:19:37 AM
CHAPTER 5 • Protein‐Based Foods: Poultry • 83
by itself has a bitter and metallic taste, so is usually used in a
blended form with sodium chloride at a ratio of 2:1 sodium
chloride to potassium chloride.
Another way to reduce overall sodium content and
enhance flavor is to add onion powder and garlic powder to
marinades. These two items add to the overall savory taste of
the product, thereby tricking the taste buds into perceiving a
salty or savory note.
Reducing sodium content also affects ingredient cost.
Sodium chloride and sodium phosphates are two of the
most commonly used and cheapest ingredients in further-
processed meats and poultry; replacing or reducing their use
with more expensive lower‐sodium options increases the
overall cost of the product.
Effect of pH on Marination Effectiveness
When developing a marinade for a particular meat product,
the overall pH is very important. Typically, poultry meat has a
slightly acidic muscle pH of 5.6–5.8. Alkaline or basic pH mari-
nades (pH of 8–9) containing water, salt, phosphate, and spice
extractives tend to absorb into the meat readily and do not
purge or weep out much over time or through the cooking
process. On the other hand, acidic marinades such as buffalo,
tequila‐lime, and lemon‐pepper often have a pH of 5–6 and
have a hard time binding with and absorbing into the muscle
tissue. The primary reason is that the more acidic the mari-
nade, the closer the muscle shifts to its isoelectric point (pI),
typically at a pH of approximately 5.1. At the isoelectric point,
the muscle proteins have no net charge due to the equal num-
ber of positive and negative charges on the muscle molecule,
resulting in poor moisture uptake and retention. This is why
acidic flavors are more successfully added as glazes, dry rubs,
or topical seasonings to chicken that has previously been mar-
inated in a more effective alkaline marinade.
Marination Methods
The process of marination can be conducted in a number of
ways. The most common commercial methods are needle
injection and vacuum tumbling. Vacuum tumbling is a widely
used technique for infusing marinades into poultry. The proc-
ess is commonly used in boneless, skinless meat items such
as breast fillets or tenderloins, and in bone‐in wing sections.
Vacuum tumbling involves placing meat and marinade in
a cylindrical container, pulling a vacuum (20–25 Hg), and
allowing the meat and marinade to tumble for 15–20 min-
utes. As the vacuum is pulled, the muscle tissue begins to
swell, allowing the marinade to penetrate the meat. Vacuum
tumbling can result in a pick‐up of 10–15 percent marinade
in only a few minutes, while static marination (submerging
meat in a marinade at ambient pressure, without tumbling)
rarely produces more than 5 percent pick‐up even after many
hours, as marinades penetrate only 2–3 millimeters into the
muscle without a vacuum.
Needle injection marination is most commonly used in
bone‐in poultry items as well as larger items (even whole
birds). It works by inserting a series of needles into the prod-
uct to directly inject the marinade deep into the muscle.
Needle injection allows greater and much faster penetration
of the marinade throughout the piece of meat. Some proces-
sors use needle injection followed by vacuum tumbling to
assure complete and uniform marinade dispersion through-
out the muscle.
Forming Strategies
Forming is used in the poultry industry to make consistently
uniform products such as nuggets, fingers, and patties. This
process, often referred to as chopped and formed, involves
grinding whole muscle pieces such as boneless, skinless
breast or thigh meat, mixing the ground meat with a season-
ing, and then placing it into a forming machine (with differ-
ent shaped plates, dies or molds) that uses pressure to stamp
out the formed product. Chopped and formed products are
usually either batter‐breaded and fried, or glazed and sold
fully cooked or ready to cook.
Salt‐soluble proteins play a major role in the develop-
ment of formed poultry products. The major salt‐soluble
proteins are myosin and actin, the proteins responsible for
muscular contraction and relaxation. Salt is added to brines
or marinades for meat to be made into formed products in
order to extract the salt‐soluble proteins. Through this
extraction process, the solubilized proteins coat all of the
meat’s surfaces and serve as an adhesive to help glue indi-
vidual chunks of meat back together. This helps the re-
formed muscles to appear whole, especially after they have
been shaped and sized to natural proportions, coated, and
fried. This is particularly important for the mass production
of products for the fast food industry (chicken patties,
Source: Courtesy of Marel Stork Poultry Processing.
FIGURE 5.4 Commercial needle injector.
c05.indd 83 1/14/2016 7:19:46 AM
84 • CULINOLOGY®
nuggets, etc.), where cost and consistent portion size are
critical factors.
Conclusion
Poultry is a major source of protein in the human diet. Of all
of the animal‐based proteins, poultry is the most versatile,
the most economical, the most heavily propagated, and also
one of the most efficiently produced proteins on a pounds‐
per‐acre basis. Unlike other meats and certain seafoods,
there are no religious proscriptions against eating poultry
anywhere in the world (except for strictly vegetarian cul-
tures). Poultry protein is easily transformed into foods as
upscale as chicken cordon bleu and as commonplace as
chicken nuggets. As such, the ability to manipulate and be
creative with poultry, particularly chicken and turkey, is of
major importance to Culinology® professionals, who will
almost certainly deal with both of these proteins at some
stage in their career.
1American Meat Institute (AMI), “U.S. Meat and Poultry Production and
Consumption: An Overview,” AMI Fact Sheet, 2009.
2Ibid.
3Ibid.
4A. R. Sams, Poultry Meat Processing (Boca Raton, FL: CRC Press, 2001).
5Ibid.
6Ibid.
7J. R. Romans, W. J. Costello, C. W. Carlson, M. L. Greaser, and K. W. Jones, The
Meat We Eat (Danville, IL: Interstate Publishers, 1994).
8 Sams, Poultry Meat Processing.
9M. W. Schilling, J. K. Schilling, J. R. Claus, N. G. Marriott, S. E. Duncan, and
H. Wang, “Instrumental Texture Assessment and Consumer Acceptability
of Cooked Broiler Breasts Evaluated Using a Geometrically Uniform‐
Shaped Sample,” Journal of Muscle Foods 14 (2003), 11–23.
10Romans, et al. The Meat We Eat.
11 Sams, Poultry Meat Processing.
c05.indd 84 1/14/2016 7:19:46 AM
CHAPTER 6 • Protein‐Based Foods: Seafood • 85
Introduction
Seafood is the only major source of protein in the mainstream
U.S. diet that is still harvested from the wild, despite the farm-
ing of shrimp, catfish, tilapia, and salmon that surpasses the
wild catches in some regions of the world. Major terrestrial
protein sources (cattle, sheep, pigs, poultry, etc.) have been
almost exclusively farm raised for hundreds of years. Globally,
due to farming, seafood production now exceeds that of beef.
Seafood differs from chicken, beef, and pork in that migra-
tory species are never guaranteed to be in a given location, or
else are seasonal. In either case, the product developer, food
manufacturer, or multi‐unit foodservice operator must
determine the amount of product needed before beginning a
project. Limitations to the available amount, size, or quality
of selected seafood will ruin any chance for success. Seafood
is a true global resource, and the impact of worldwide
demand on the availability of stocks must be taken into
account. In many cases, the most coveted sizes of fillets and
shrimp are relatively expensive, and large users, such as chain
and club stores, may have existing supply contracts. Begin
each project with an assessment of what is available in the
required size and price range. This sounds rudimentary but
reinforces the observation that seafood is unlike any other
protein used by the product developer.
Seafood Classifications
Tens of thousands of species of edible sea life are known, but
only about 1500 are harvested globally, with approximately
400 species of finfish and 80 species of shellfish commercially
harvested in the United States. 1 Millions of tons of seafood are
served annually. The most popular seafoods in the United
States are shrimp, canned tuna, salmon, pollock, and tilapia. 2
Seafood can be generally classified along three criteria:
1. Vertebrate or invertebrate: Vertebrates include those spe-
cies with internal skeletons (sharks have cartilage rather
than bone), and these species may or may not have scales.
Examples of vertebrates with fins are halibut, cod, salmon,
flounder, tuna, eels, and swordfish. Turtles, alligators, and
frogs are also considered seafood (21CFR123.3) and are
vertebrates with neither fins nor scales. Invertebrates ,
commonly referred to as shellfish , are divided into two
groups: crustaceans (crab, lobster, crawfish, and shrimp),
which all have an exoskeleton; and mollusks , which are
further divided into three subgroups: bivalves (clams,
oysters, scallops, and mussels), gastropods (abalone,
snails, whelk, and conch), and cephalopods (squid,
cuttlefish, and octopi). Other invertebrates used for
food include jellyfish ( Scyphozoa ), the sea cucumber
( Holothuridea ), and the sea urchin ( Echinoidea ).
2. Saltwater (and brackish water) or freshwater: These species
may spend their lives in the ocean (cod); may be born in
fresh water, live their lives at sea, and return to the fresh
water to spawn (salmon); or may live their entire lives in
fresh water, such as large‐mouth bass, trout, walleye, pike,
and catfish.
3. Lean or fatty: Lean species contain 2 percent or less total
lipid (cod, flounder, lobster). Fatty species, such as wild
salmonids, sablefish, mackerel, herring, and anchovy, can
vary greatly in total lipid content depending on the season
and whether or not they have been feeding heavily.
Lipid content ranges from 5 percent (cultured salmon)
to 22 percent (sablefish, also known as black cod or
butterfish).
Market Forms
of Finfish
Finfish are available fresh or frozen in several forms: whole
fish (entirely intact), drawn fish (whole with entrails removed),
dressed fish (entrails, head, tail, fins and scales removed),
steaks (crosscut slices of varying thickness from dressed fish;
contains a piece of backbone in the center of each slice), fillets
(boneless flesh of the fish sliced front to back by cutting
lengthwise along each side of the fish parallel to the back-
bone; may or may not be skinless).
Lead Author: Lucina E. Lampila, Ph.D., R.D., C.F.S., Senior Food Scientist, Evergreen Packaging
Contributor: Jeff Cowles, CCS® , Research Chef, Kerry Americas
6
Protein‐Based
Foods: Seafood
c06.indd 85 1/22/2016 1:01:24 PM
86 • CULINOLOGY®
Muscle Structure
of Finfish
Proteins derived from seafood have biochemical characteristics
different from those of their terrestrial counterparts (poultry,
pork, and beef ). In most cases, their structure is far more deli-
cate and must be treated with greater care.
Finfish have one long lateral muscle, unlike land animals,
which have muscles of varying length and strength, depend-
ing on their function. Finfish muscle fibers run parallel to the
backbone of the fish. Unlike the bundles of muscle fibers in
meat (up to 3 feet in length) and poultry, fish muscles are
made up of layers of short fibers (less than an inch in length,
or a bit longer) called myotomes. These layers contribute
to the characteristic flakiness of fish as it cooks. The number
of myotomes corresponds to the number of vertebrae in the
fish’s backbone,3 with the average fillet containing 50 myo-
tomes. Myotomes are separated from each other by thin
connective tissue, or myocommata, analogous to the con-
nective tissue collagen in terrestrial animals. Myocommata
is more densely concentrated at the tail of the fish, where
strength is required for locomotion. Therefore, the eating
quality of finfish is least desirable close to the tail. Seafood
connective tissue does not become as highly cross‐linked as
that of beef, so toughness is rarely an issue. With the
exception of the pedal sole (the inedible bottom of the foot)
of the abalone, the content of connective tissue is 0.2 to 2.2
percent in most seafood,4 as opposed to about 15 percent in
mature beef.5 The amount of cross‐linking increases with
age, which leads to increased toughness in meat.
Identifying Freshness
in Seafood
One commonality of finfish of marine (saltwater) and fresh-
water origin is the fundamental characteristics of post‐
harvest quality determination. Some species may be more
resilient than others; however, patterns of deterioration are
typically indicative of eating quality.
The most obvious sign of freshness is aroma: as fish ages it
rapidly develops an objectionable odor that is immediately
apparent. In addition, fresh fish should have tightly fitting
scales, firm yet resilient flesh that “bounces back” when
depressed, protuberant and translucent corneas free from
any cloudiness, and bright red gills.
Rigor and pH
As a rule, living finfish have a pH of 6.2 to 7.2 and an ultimate
pH of 5.8 to 6.2 within hours of death. If fish are caught while
heavily feeding or post‐spawning, high glycogen reserves
result in large drops in pH (due to the postmortem formation
of lactic acid via anaerobic glycolysis) that lead in turn to pro-
tein damage and moisture loss. As pH departs from neutrality
and approaches the isoelectric point of the protein (the point
Source: Sea Grant College Program.
FIGURE 6.1 Recognizing finfish quality.
Select for Fresh Whole Fish Avoid
Clear, bright, full, often protruding EYES Cloudy, pink, sunken
Red, free from mucous or slime GILLS With age fades to pink to gray and eventually
brown or green; strong odor
Fresh, mild, slight seaweed ODOR Strong odor, ammonia‐like, putrid
Shiny with vibrant color SKIN Dull, faded color
Firm and elastic to the touch FLESH Soft, separating from the bone
Clean cut, free of viscera; lining
intact, clean aroma (if gutted)
BELLY CAVITY Belly burst (rupture of cut through the flesh if
not eviscerated); bones projecting through the
cavity lining; yellow, orange, brown or green
discoloration; objectionable odor
Fresh Fillets and Steaks
Appearance should be freshly cut,
texture should be firm and elastic
FLESH Gaping (a separation between) of muscle bun-
dles; protruding bones; soft or mushy texture or
desiccated (dried out) appearance
Fresh, mild, seaweed‐like ODOR Ammonia‐like, putrid, strongly fruit‐like
Bright, shiny, consistent COLOR Dull and grey to tan, which is indicative of desic-
cation; reddish‐brown discolorations, which are
indicative of blood spots; yellow to orange to
brown edges, which are indicative of rancidity of
the fish oils
c06.indd 86 1/14/2016 7:21:19 AM
CHAPTER 6 • Protein‐Based Foods: Seafood • 87
at which the protein has no net charge, or the least functional-
ity), it can no longer bind water.
If fish are caught during spawning (e.g., wild salmon), lipid
content (due to gonad development), moisture content (as
unbound water), and free amino acids are elevated, with a
concomitant reduction in both myofibrillar protein (the
muscle protein fraction, including actin and myosin, that
are functional proteins—that is, that binds water and is
extractable by strong salt solutions) and sarcoplasmic pro-
tein (the muscle protein fraction, including enzymes and
myoglobin, that is soluble in water or dilute salt solutions).6
The ultimate pH in these fish does not drop as low as usual,
and the higher pH leads to more rapid onset of bacterial
spoilage. The increased moisture in the presence of reduced
myofibrillar protein tends to allow for migration of sarco-
plasmic protein to the surface, where it coagulates upon
heating. This coagulum resembles a dry curd that may be
visually unappealing. A similar curd results when salmonids
are frozen and subsequently canned.7 It is important to note
that salmon are graded for quality, and those that appear
spent (show signs of deterioration) are diverted to fish meal
or other by‐product uses.
Finfish proceed through rigor mortis similarly to terres-
trial animals; the key difference is the speed of each phase. In
typical finfish, onset of rigor occurs in 1 to 2 hours, and reso-
lution is complete within several hours. Very large species,
such as bluefin tuna, take longer to undergo rigor; whales
take up to 50 hours. Most animal proteins are prepared for
consumption after rigor is resolved and the muscle tissues
have relaxed. The exception is in Asia, where it is not uncom-
mon for a seafood species to be selected live from a tank,
slaughtered, and consumed pre‐rigor. The texture then is
very tender and delectable.
Immediately after death, finfish are limp and pliable, and
the flesh is elastic. Most finfish species begin stiffening at the
head and progress to the tail. As this occurs, actin binds to
myosin, with a shortening of the myotomes, which ranges
from 5 to 15 percent but may be significantly higher in deep-
water species.8 When the fish is in full rigor, it is contracted to
the greatest extent; it stiffens to the point where it may
appear concave when laid on its side on a flat surface.
During full rigor, tension may be sufficient to rupture the
myocommata and cause gaping, a noticeable void between
myotomes (separations or gaps in the cut surface of a fillet).
Gaping may also be caused by attempts to straighten a fish in
full rigor, by rough handling, after filleting a fish in rigor, or by
inadequate chilling. Fish should not be frozen before or dur-
ing rigor because this leads to thaw rigor: Upon thawing,
rigor continues with an exaggerated muscle contraction that
causes excessive drip loss and dryness of the flesh. The two
exceptions to this rule are long line–caught fish of high value
that can be frozen pre‐rigor (such as swordfish and tuna), and
aquacultured finfish that may be processed immediately.
These two types are well nourished and not exhausted (no
stress resulting from struggling at catch and consequential
depletion of glycogen reserves); they pass through rigor later
without excessive muscle damage.9
After the resolution of rigor, the pH of the fish rises to 5.8 to
6.2, although those fish entering rigor at a higher pH usually
show a final pH above this range. As with all seafoods, there
are exceptions, and some values may be higher than usual but
typically not above pH 6.7. The higher pH leads to more rapid
onset of bacterial spoilage.
Fresh Finfish Protein Quality
The functional quality of the salt‐soluble protein of finfish is
extremely high immediately after catch. With time, even on
ice where the temperature is maintained at 33°F (<0.5°C), the
extractability of the salt‐soluble protein (the myofibrillar pro-
teins composed of actin and myosin) decreases. By six days
after catch, fresh West Coast rockfish (Sebastes species) held
at 39°F (4°C) show a 73 percent reduction in salt‐soluble pro-
tein.10 This is significant because the protein responsible for
keeping the flesh moist is compromised. This becomes evi-
dent by increased drip loss and toughening of the flesh,
accompanied by dryness or loss of succulence. Fishermen and
processors are frequently accused of not meeting net stated
weight (the Food and Drug Administration [FDA] terms this
economic fraud) when in reality the increased drip loss is a
natural phenomenon. Attempts to overpack in order to com-
pensate for drip loss are difficult because of the large variation
in stability of myofibrillar protein among finfish families.
Protein Measurement
Total protein varies widely in wild species, ranging from 7 to 24
percent. Mollusks, such as oysters and clams, tend to contain
lower protein, while albacore, halibut, and skipjack tuna tend
to contain higher protein. Digestibility of seafood protein typi-
cally exceeds 90 percent, with a high PER (protein efficiency
ratio). Unlike terrestrial animals, most fish species contain
appreciable levels of non‐protein nitrogen compounds, which
may include trimethylamine oxide and its downstream prod-
ucts, urea (especially in sharks and rays), taurine, peptides, free
amino acids, nucleotides, purines (adenosine triphosphate
[ATP], the energy storage compound), and products of decom-
position. For this reason, total protein cannot be determined
directly by either of the two analytical methods used most
widely for protein determination, Kjeldahl Digestion11 or the
more rapid Combustion Method. Both measure total nitrogen
present in a sample, but because non‐protein nitrogen is meas-
ured along with nitrogen from proteins, the results must be
corrected for non‐protein nitrogen to be accurate. The
Combustion Method uses a conversion factor of 5.8 × N
(N = nitrogen) for seafood, compared to 6.25 × N for terrestrial
meats.12 The product developer must keep this in mind when
designing a nutrition panel. Failure to do so leads to a falsely
high apparent protein content.
Trimethylamine Oxide and Ammonia
Gadoids (finfish of the family Gadidae, which includes
pollock, cod, whiting, hake, cusk, and turbot) contain
extremely high levels of trimethylamine oxide (TMAO, an
c06.indd 87 1/14/2016 7:21:19 AM
88 • CULINOLOGY®
osmoregulatory compound that works to balance osmotic
pressure and to regulate buoyancy of the fish). Gadoids
also have the enzyme trimethlyamine oxidase (TMAOase),
which accelerates the breakdown of TMAO to trimethyl-
amine (TMA), the highly aromatic compound most com-
monly associated with fish decomposition (fishy smell).
TMA is hydrolyzed to DMA (dimethylamine) to MMA
(monomethylamine) to FA (formaldehyde). Formaldehyde
(not at levels harmful to humans) denatures the protein
and results in excessive cook loss, toughening of the flesh,
and decreased succulence. TMAOase is also active at fro-
zen temperatures, and this hydrolysis cascade occurs with
rapid toughening of the flesh (within six weeks at high,
14°F [–10°C] frozen storage temperatures).13 Gadoid species
naturally have a short shelf life if stored raw, but this can be
extended by par‐cooking in order to denature the enzymes.
The breakdown of TMAO also occurs in nongadoids by the
action of spoilage bacteria of the genera Alteromonas,
Photobacterium, and Vibrio.
Elasmobranchs (sharks, dog fish, skates, and rays) contain
high levels of urea as an osmoregulator. Shark flesh has some
desirable eating qualities; however, if not bled after catch,
urease‐positive microorganisms convert the urea to ammo-
nia, which has a highly objectionable odor.14 Ammonia is also
a decomposition product in other seafood species (shrimp,
crab, and finfish).
A simple but effective way to determine the decomposi-
tion (freshness) of seafood is:
1. Place a small amount (about 1 ounce) into a plastic seal-
able bag and seal.
2. Place the sample in a microwave oven and cook for 30 to
60 seconds on high, or until the bag begins to puff and is
full of steam.
3. Immediately remove the bag, open it, and carefully sniff
the steam coming from the seafood. If ammonia, muddi-
ness, algae, or other off aromas are present, the smell will
be obvious.
4. If any unpleasant odors are detected, discard the seafood.
Fat Content
In finfish, fat content can vary significantly within species
depending on spawning, feeding, and migration patterns.
Albacore tuna in the far Pacific tend to be lean or very low
(1 percent) in fat content, while the same species feeding in
coastal waters tends to deposit lipids in significant quantities
(16 percent). Those tuna that are fattier and caught close to
shore are superior for preparing smoked and pickled prod-
ucts, or for consumption as sashimi or ceviche. The elevated
fat content is ideal for absorption of flavor compounds (such
as smoke and spice), and it allows for a tender pickled prod-
uct without toughening due to the reduced pH caused by vin-
egar. Belly flaps tend to harbor fatty tissue and are the most
delectable part of smoked and pickled fish.
Note: The omega‐3, ‐6, and ‐9 fatty acid levels in fish are
dependent on the composition of the fish’s dietary intake.
It is incorrect to assume that all marine species are good
sources of the heart‐healthy omega‐3 fatty acids. Fish such
as the sablefish (Anopoploma fimbria) and spiny dog fish
(Squalus acanthias) instead may contain as much as 70 per-
cent omega‐9 fatty acids (oleic acid, present at high levels in
olive oil and part of the Mediterranean diet).15 Sablefish,
also known as butterfish or black cod, have, when smoked,
a consistency that is almost buttery or pâté‐like, and, when
pickled, a texture that defies a description other than
marvelous.
Marine fish chilled in refrigerated sea water (RSW) or
seawater‐based ice slush, or frozen in a concentrated
(23 percent) brine, absorb sodium chloride (NaCl). The
quantity of NaCl is greatest in fish frozen and held in con-
centrated brine (wet holding), next‐highest in fish frozen in
concentrated brine and drained after freezing (dry holding),
next‐highest in fish held for a day or two in RSW, and least
in fish that are briefly chilled in slush. This is significant
because NaCl is a pro‐oxidant of the polyunsaturated fatty
acids present in fish oils.16 Phospholipids, especially phos-
phatidylcholine (lecithin), are the most susceptible and the
first to undergo oxidation. Breakdown products of lipid oxi-
dation (aldehydes) combine with proteins and render them
hydrophobic17—that is, causing them to repel water and
toughen the fish flesh. The product developer is probably
best served by specifying that frozen tuna for product devel-
opment never be held wet for long periods after freezing, as
that flesh is fit only for canning.
Dark and Light Flesh
Dark fish flesh is readily distinguishable and usually concen-
trated adjacent to the backbone or along the sides in either
narrow bands or in wide zones. The dark flesh is used for
continuous motion, while the light flesh is used for sudden
bursts of speed.18 Similarly, poultry muscle contains more
dark tissue in those areas (legs and thighs) used for continu-
ous locomotion. Dark meat in fish is due primarily to the
presence of myoglobin (the iron‐containing pigment that
stores oxygen in the muscles for respiration) and hemo-
globin.19 The dark flesh not only contains myoglobin that can
oxidize to metmyoglobin and form a brown discoloration,
but it also tends to carry higher levels of lipid in the form of
polyunsaturated oils that can quickly oxidize in the presence
of the iron (an oxidation catalyst). This results in a strong,
often objectionable flavor, which is the reason domestic con-
sumers often remove the dark flesh before cooking or scrape
it off after cooking.
Tuna species have this dark flesh immediately under the
skin, at the center of the loin, and along the backbone.20 In
canneries, tuna is precooked so this dark flesh can more
easily be separated from the light meat and sent for pet
food processing. In Asia, however, properly handled dark
flesh is prized for sashimi. In the United States, it is now
common practice to treat tuna and mahi loins and por-
tions with carbon monoxide or tasteless purified smoke to
form carboxymyoglobin, providing a vibrant red color.
c06.indd 88 1/14/2016 7:21:19 AM
CHAPTER 6 • Protein‐Based Foods: Seafood • 89
Adenosine Triphosphatase
(ATPase) Test for Freshness
Adenosine triphosphatase (ATPase) is the enzyme that
hydrolyzes ATP, the energy storage compound in muscle that
allows dissociation of actin from myosin in the actomyosin
complex (causing the muscle to relax). Postmortem, ATPase
is not regenerated, and its loss is directly correlated with loss
of protein functionality. The higher the content of residual
ATPase, the greater the ability of the actomyosin complex to
dissociate. The presence of residual ATPase is also positively
correlated with water‐holding capacity and, in re-formed
products (such as surimi), the strength of the gel formed on
heating. ATPase breakdown slows at colder holding tempera-
tures: 32°F/0°C > –0.4°F/–20°C > –40°F/–40°C > –112°F/–80°C.
That said, while holding seafood at ultralow temperatures is
common practice for laboratory samples, it is not realistic for
commercial settings.
The Japanese are connoisseurs of sashimi and have con-
ducted some of the best and most detailed studies to deter-
mine finfish quality. ATP is hydrolyzed postmortem to
adenosine triphosphate (ADP) to adenosine monophosphate
(AMP) to inosine monophosphate (IMP) to inosine (INO) to
hypoxanthine (Hx) to uric acid (U). A complex equation has
been developed involving ATP and its downstream products
to determine the K‐value, which is used to predict the quality
of fish sold for sashimi.21 The K‐value is species‐specific because
it increases dramatically depending on the species. For exam-
ple, Fraser et al. determined that IMP was depleted in iced
cod in 9 days.22 Spinelli demonstrated the presence of IMP in
iced halibut at 41 days.23 For optimal‐quality sashimi, the
K‐value should be 3.5 percent immediately after catch, less
than 20 percent for good quality, and about 50 percent for
medium quality.24 This is one of the means used by the
Japanese to determine the quality (and price) of sashimi.
Preserving Seafood
The supplier of fresh, never‐frozen seafood should be able to
state where it was caught or aquacultured and the methods
used to ship the product. Seafood sold at retail must be
labeled to indicate if it was wild caught or aquacultured
(7CFR60.200). An abundance of ice surrounding the seafood
within the pack should keep the temperature close to 32°F
(0°C) to retard spoilage. There should be no question that the
seafood was treated with respect and urgency for its delivery.
Seafood destined for raw consumption (i.e., for sashimi,
ceviche, or crudo) requires special handling, discussed fur-
ther in the Fish‐ and Shellfish‐Derived Foodborne Illnesses
section below.
Fresh seafood offers a distinctive eating experience that
elevates the consumers’ experience and produces a greater
respect for the product, which results in a higher selling price
than most previously frozen product (sashimi notwithstand-
ing). That said, a variety of shelf life extenders, including
freezing, canning, salting, and packaging strategies, are used
to extend seafood shelf life.
Freezing
Most commercial processors of raw seafood do an excellent
job of freezing fish. Rapid freezing produces a superior product
when thawed and eaten. In many cases, commercially
processed seafood that is frozen at sea has better sensory
attributes than its fresh, unfrozen counterpart. Most wild or
aquacultured fish are frozen within minutes of being caught or
harvested. Therefore, it is possible to catch high‐quality sea-
food in remote locations, freeze it quickly, and ship it rapidly to
the distributor, retailer, and finally, the consumer.
Once‐frozen seafood is the primary process used in the
industry. In this technique, wild‐caught or aquacultured sea-
food is trimmed or portioned shortly after harvest and imme-
diately frozen. Many times this occurs at sea on the harvesting
vessel or within a few hours’ drive of the aquaculture ponds.
The seafood is not thawed until use.
Twice‐frozen seafood refers to wild finfish that are caught,
headed and gutted (H&G), and frozen at sea. The H&G frozen
finfish are shipped to a processing plant where they are care-
fully thawed, filleted, portion‐cut, and then refrozen. This
process is safe and can produce uniform hand‐cut portions
available for breading, further processing, or cooking. The
care taken in thawing, trimming, and refreezing determines
the quality of the finished product. It is possible to source
twice‐frozen products of the highest quality. Some of these
may contain added ingredients (typically, phosphates) to pro-
tect protein and to maintain moisture during freezing and
cooking.
Freezing temperature affects ice crystal formation. The
higher the freezer storage temperature, the longer it takes
products to freeze, and the larger the ice crystals that are
formed. As water freezes, it expands and can rupture cell
membranes, resulting in increased drip loss on thawing.
Temperature rise during frozen storage results in changes to
the size of the ice crystals. For example, if a fillet is frozen to
–40°F (–40°C) and later moved to a 0°F (–17.8°C) freezer, the
ice crystals will grow or accrete.25 This can also occur in frost‐
free freezers, where warming and cooling cycles occur
mul tiple times each day to prevent accumulation of excessive
FIGURE 6.2 Freeze temperatures: percentage of
water frozen in finfish as a function of temperature.
Temperature of Flesh
°F (°C)
Water Frozen
(%)
30.4 (–0.9) 0
30 (–1.1) 32
28 (–2.2) 61
26 (–3.3) 76
24 (–4.4) 83
22 (–5.6) 86
18 (–7.8) 89
Source: Lucina Lampila.
c06.indd 89 1/14/2016 7:21:19 AM
90 • CULINOLOGY®
ice deposits inside the chamber. Packaged seafood subjected
to excessive freeze‐thaw cycles accumulates large ice crystals
between the frozen flesh and the packaging material. This is
moisture from the flesh that has sublimated (passed from a
solid to a vapor and back to a solid state) into the headspace
between the fillet and the packaging material. The result is
freezer burn, featuring significant surface dehydration and
loss of overall eating quality. If frost‐free storage is the only
option, it is best to vacuum package the seafood to eliminate,
as much as possible, any headspace, or to overwrap frozen
0
5
10
15
20
25
30
35
40
45
50
T
e
m
p
e
ra
tu
re
˚
F
Hours
Brine
Water
6 66 84
FIGURE 6.3 Freezing curve. This figure shows the rate of freezing of whole bonito (Sarda chiliensis) in
either a eutectic (NaCl) brine or potable water.
Source: Lampila and Rittmanic, unpublished data.
FIGURE 6.4 Micrograph: ice crystals. This
micrograph shows the sites of presumed ice
crystals and the damage mediated as they
accrete or grow after the rockfish (Bocaccio,
Sebastus paucispinis) fillet is moved from –4°F
(–20°C) to 23°F (–5°C). The sharp edges of the
ice crystals presumably rupture the cell and
exacerbate the loss of natural fluids observed
as thaw‐drip loss.
Source: Lampila et al., 1985.
FIGURE 6.5 Micrograph: crushed muscle. This
piece of rockfish (Bocaccio, Sebastus paucispinis)
fillet was frozen at –4°F (–20°C). After 10 days in
frozen storage, the crushing of the muscle struc-
ture can be observed in this cross section. These
two micrographs (Figures 6.4 and 6.5) were taken
of rockfish flesh preserved by isothermal freeze
fixation, a process whereby the flesh was chemi-
cally fixed at the frozen storage temperature to
the size and shape of ice crystals.
Source: Lampila et al., 1985.
c06.indd 90 1/14/2016 7:21:23 AM
CHAPTER 6 • Protein‐Based Foods: Seafood • 91
packages with several layers of paper for insulation against
the freeze‐thaw cycling.
During the freezing process, water slowly migrates out of
the cells, causing salts normally present in the interior of the
cells to become more concentrated. This concentration may
result in the salting out of proteins, which can accelerate
denaturation. Salting out precipitates proteins from solution
as ionic strength increases. Over time, this can cause the pro-
teins to irreversibly denature. A denatured protein has an
unfolded structure, and many of its functional properties are
compromised or eliminated—in particular, its ability to bind
water, leading to excessive drip loss and dry texture upon
thawing.
Several other problems are indicated by thawed fish fillets
that drip excessively or have flesh that becomes chalky or
rubbery, or pulls into ridges. Chalky flesh is usually freezer
burned; rubbery flesh was frozen too long for its species type,
and flesh pulling into ridges is indicative of thaw rigor (due
to fish being frozen before going through rigor mortis). Cod
and pollock are among the species with a very short frozen
shelf life.
It is important to note that some enzymes, such as
TMAOase (the enzyme responsible for the formation of
trimethylamine—one odorant responsible for the fishy smell
in spoiling seafood), are still active at normal frozen tempera-
tures (i.e., between 0 and 32°F [–18 and 0°C]). This is why
commercial freezers are maintained at –20°F (–28.9°C) or
colder.
This is not to say that finfish should not be frozen. On the
contrary, freezing finfish quickly and to a low temperature
maintains the quality of the flesh closer to the point of fresh-
ness. That said, it is critical to package the product so oxygen
is excluded or under vacuum, and that the packaging mate-
rial has low oxygen permeability. The polyunsaturated fatty
acids in fish oils are highly subject to autoxidation (the oxy-
gen‐mediated breakdown of lipids resulting in free radical
formation and rancidity) even when frozen. And as mentioned
previously, breakdown products of lipid oxidation (aldehydes)
combine with proteins and render them hydrophobic, causing
them to repel water and toughen the fish flesh.26 Exclusion
of oxygen slows this reaction.
Seafood can be packaged so each individual piece or
portion is separated while frozen. Layers of plastic can
separate each fillet (cello‐pack, vacuum pack, or shatter-
pack), or seafood pieces can be individually quick‐frozen
(IQF). Fillets can also be frozen into blocks that facilitate
cutting the fish into portions for breading, par‐frying, and
freezing.
Thawing Vacuum‐Packaged Seafood
Vacuum packaging introduces an anaerobic (oxygen‐free)
environment. Clostridium botulinum Type E and non‐pro-
teolytic Types B and F are anaerobic, spore‐forming bacterial
pathogens most often associated with raw seafood. This type
of C. botulinum is unusual in that it tolerates lower tempera-
tures than others. Vacuum‐packaged raw fish must be stored
at less than 37.9°F (3.3°C), according to the FDA’s recom-
mended handling practices, as this prevents the germination
of spores (which results in toxin production) and growth of
the organism. Some states have regulations that require any
vacuum‐packaged raw seafood to be kept frozen. With all
types of frozen, vacuum‐packaged seafoods, it is recom-
mended that the packaging be pierced (or removed entirely)
to release the vacuum and to introduce oxygen before thaw-
ing. The FDA (2011) recommends that all frozen, reduced‐
oxygen‐packaged seafood bear the warning “Important: Keep
frozen until use. Thaw under refrigeration immediately
before use.”
Canned Fish
Per‐capita consumption of canned fish in the United States
was 3.9 pounds in 2010.27 Nearly 70 percent of all canned
fish consumed in the United States is tuna. Shellfish and
other fishery products (mackerel, anchovies, clam juice, etc.)
account for 10 percent each of canned products, with salmon
and sardines accounting for about 5 percent each. True can-
ning (retort sterilization) makes fish shelf stable at room tem-
perature, and the shelf life is in years. Pasteurized canned
crab is not shelf stable and must be refrigerated. Pasteurization
extends the refrigerated shelf life of crab to approximately six
months.
Several species are permitted by the FDA’s Standard of
Identity (complete information can be found at: 21CFR161.190)
for canned tuna to be labeled as light‐meat tuna, including
bluefin, yellowfin, blackfin, bigeye, and skipjack, but only one,
Thunnus alalunga (Bonnaterre, 1788), or albacore, is permitted
for use as white‐meat canned tuna.
Albacore tuna feed near the top of the predatory chain
and as a consequence bioaccumulate higher levels of methyl
mercury than other species of tuna; this sort should be eaten
sparingly by pregnant women and young children. Skipjack,
on the other hand, neither live long enough nor grow suffi-
ciently large (average size is less than 10 pounds) to bioaccu-
mulate dangerous levels of methyl mercury, so, generally
speaking, light tuna is a much safer option for children and
pregnant women. Skipjack comprises approximately 70 per-
cent of all light‐meat canned tuna.
Chinook Oncorhynchus tshawytscha (king) salmon is the
most expensive variety of salmon. It is primarily used for fresh,
frozen, and hot‐ or cold‐smoked products (including “lox”).
Less expensive varieties are sockeye or red salmon
(Oncorhynchus nerka), coho (Oncorhynchus kisutch), pink
salmon (Oncorhynchus gorbuscha), and chum (Oncorhynchus
keta), which are more often canned—with or without the
bones. If salmon is packed with the bones, they are softened in
the retort (commercial pressure cooker) to the point where
they become edible and contribute calcium and phosphorus
as nutrients.
c06.indd 91 1/14/2016 7:21:23 AM
92 • CULINOLOGY®
Other seafoods commonly available in canned form
include sardines, mackerel, oysters, mussels, octopus, kippers
(smoked herring), and anchovies.
Restructured Fish
One means of restructuring fish is to transform fish fillets
into uniformly portioned products, such as fish sticks or nug-
gets. Cleaned fish fillets are frozen at sea into 40‐pound
blocks. Once landed, the blocks are tempered and sawed into
standard portions. They can then be breaded and fried or
par‐fried, then refrozen for retail or foodservice sale.
A second means of restructuring fish involves the produc-
tion of surimi. Surimi, originally of Japanese origin, is a gelled
paste made from fish used to mimic the appearance, tex-
ture, and color of more expensive lobster, crab, and other
shellfish. The most common surimi product in the Western
market is imitation crab meat (also known as “crab stick”).
Surimi is made from lean, light‐fleshed fish that are
mechanically deboned and minced. The minced flesh is
typically washed two to three times in chilled potable water.
Because the flesh absorbs water during washing, it is de‐
watered and then screen‐refined to remove small pieces of
skin, connective tissue, and bone. The washed, refined fish
mince is then mixed with cryoprotectants (4 percent each
of sucrose and sorbitol, plus 0.5 percent of a blend of phos-
phates) and frozen into 44‐pound blocks for later restruc-
turing. Low‐cost species (pollock or Pacific whiting) must
be used because the yield is less than 30 percent of the
whole fish. Later, the frozen block is tempered and blended
with potato starch, dried egg white, flavorings, salt, and
phosphate to prepare the analog. The blended fish paste is
extruded onto a belt, steam cooked, and cut into fibers. The
fibers are then cut and rolled with more of the paste as a
binder, and steam cooked again. A food‐grade red color is
typically sprayed onto the product to provide pigmentation
characteristic of the intended end species. In addition to
use as low-cost crabmeat replacements (so‐called krab or
sea legs), surimi is also available as shrimp and lobster ana-
logs used in salad bars.
Salting: Caviar and
Preserved Roe
Caviar is the processed roe of any species of sturgeon or
paddlefish (order Acipenseriformes, 50CFR23.71). The most
expensive (hundreds to thousands of dollars per pound) is
Russian or Iranian black Beluga caviar, which comes from the
black sturgeon (also caught in the United States, but not
commercially for the roe). Other fish eggs are also harvested
and sold as caviar, but the species (salmon, lumpfish, flying
fish, etc.) must be named on the label. Sea urchin roe,
extremely popular in Japan for many years (where it is called
uni), became trendy in the United States with the exploding
growth of sushi restaurants.
In finfish, the roe is contained in skeins or sacs. It is not
uncommon for the Japanese to fly in workers to harvest the
skeins from salmon (for example) before the fish are filleted
at the processing facilities. The skeins are carefully placed in
sturdy packaging and flown to Japan. In the United States,
depending on the species, the skeins may be broken by hand
and the eggs removed, or the skeins may be rubbed against a
screen to loosen the eggs. The eggs are then hand culled to
remove bits of membrane, blood, intestine, or black skin. The
roe is held in a salt solution for 1 to 5 hours (depending on the
species), rinsed, drained, and packed into a container and
refrigerated.28 Alternatively, the roe may have salt added
directly with mild agitation until it is dissolved. If the intent is
to hold the roe for any length of time, it is critical to assure
that water activity is reduced to a
w
<0.97 and/or that the
water phase salt is sufficiently high (5 percent) to inhibit
growth of C. botulinum by the reduced-oxygen environment.
Roe can also be salted and dried, as is commonly done in Italy
in the preparation of bottarga, salted and pressed roe of gray
mullet, bluefin tuna, and other fish species.
Treating Seafood
with Phosphate
As with all proteins, when used properly, phosphates allow
seafood to stay moist and succulent after cooking. When sea-
food is breaded or battered and fried, phosphates help retain
moisture as well as the shape and size of the original
uncooked protein. This improves the moistness of the cooked
product, keeps the seafood from pulling away from the bread-
ing (creating a shell effect), and enhances the holding quality
of the cooked product. With the increasing volume of twice‐
frozen products into the marketplace, phosphate plays an
important role in keeping these products moist when held
cooked or eaten. Maintaining protein hydration also helps
maintain natural color and flavor. Alkaline phosphates also
raise the temperature for denaturation to occur.29 Thus,
phosphate‐treated seafood products are more forgiving to
overcooking.
As previously indicated, the level of salt‐soluble protein
(the myofibrillar or functional protein) is an indicator of eat-
ing quality. This is the same protein that binds water to form
a sol (protein hydration, dispersion, and swelling) and, after
heating, a gel. Water binding is positively correlated with juic-
iness and tenderness of the cooked protein. Salt‐soluble pro-
tein also interacts with fats and oils in the development of
emulsions and contributes to emulsifying capacity. The food
developer must keep in mind that myofibrillar protein is best
solubilized by low levels of sodium chloride together with
sodium tripolyphosphate to entrap moisture and lipids in a
stable protein matrix upon heating, thus ensuring maximum
eating quality.
The optimal concentration of sodium chloride for extract-
ing the salt‐soluble proteins is 3.5 percent (0.6M) salt.30
However, this level of salinity is unpalatable to many
c06.indd 92 1/14/2016 7:21:23 AM
CHAPTER 6 • Protein‐Based Foods: Seafood • 93
individuals, and the public has been encouraged to consume
less sodium. Commercially, salt‐soluble protein is extracted
with a combination of 1.4 to 1.8 percent salt31 plus 0.4 to 0.5
percent alkaline phosphate (typically sodium tripolyphos-
phate [STPP] or combinations of STPP plus tetrasodium
pyrophosphate [TSPP],32 with lesser quantities of sodium acid
pyro phosphate [SAPP]). Salt and the alkaline phosphates
work synergistically (less salt is required with a low level of
phosphate, and the impact of the two ingredients is greater
than the sum of the two ingredients added alone). Each ingre-
dient extracts different regions of the sarcomere (individual
sub unit of myofibril) and results in enhanced salt‐soluble
protein solubility. In addition, salt is a pro‐oxidant (it pro-
motes oxidation); STPP negates this oxidative effect, thereby
prolonging product shelf life.33
Condensed (long‐chain) alkaline phosphates alone are
also used as cryoprotectants in finfish. The fillets are con-
veyed through a solution of STPP, with a flex in the belt to
expedite penetration between myotomes on the upper and
lower sides. This creates a surface coating of protein that
acts as a glue to allow adhesion of fillets to each other when
block‐freezing. This process eliminates voids that could har-
bor air pockets during freezing and, if destined for standard
portion cutting, holes in the portion. Similar protocols are
also used on fillets to be sold as fresh to better predict and
control the amount of overpack needed to meet net stated
weight.34
Phosphates may be present in purchased commercial
marinades or added to those prepared on site. It is important
never to use copper or aluminum ware to either prepare solu-
tions or hold product while marinating. Copper interacts
with the phosphate and forms a green color on the fish, and
although not harmful, the result is visually unappealing.
Aluminum vessels can pit and in severe cases develop pin-
hole leaks. It is also important to know the water quality used
in food preparation. Hard water containing appreciable lev-
els of minerals, especially copper, iron, and manganese, used
in phosphate‐based marinades may result in discolorations
to the finished food products. Hard water containing 200
ppm or more of calcium and magnesium may also form pre-
cipitates with condensed phosphates, which can clog injec-
tor needles or leave surface residue.
Similar to its use in meat and poultry, phosphate use in
seafood products is limited by the FDA to 0.5 percent in the
finished food. However, even though long‐chain potassium
phosphates provide excellent water‐binding properties, at
levels greater than 0.3 percent, potassium phosphates may
contribute a bitter flavor (similar to when potassium chloride
is used as a substitute for table salt). Sodium phosphates used
in excess may contribute a slightly soapy note to the flavor.
Further, fillets overtreated with alkaline phosphates feel slip-
pery or slimy. Phosphate added in excess to seafood tends to
cause the protein to absorb and hold an unnatural amount of
water. Shrimp and finfish overtreated with solutions of salt
and alkaline phosphate look glassy and have a rubbery tex-
ture, and the flesh resists becoming opaque despite extended
heating. Phosphate producers have long encouraged the
responsible and appropriate use of their products for main-
taining the quality of seafood products.
Economic Fraud and
Species Substitution
Species substitution is considered economic fraud. This is a
criminal act, and the FDA encourages prosecution of vendors
taking part in species substitution. Figure 6.6 shows some of
the most commonly substituted species, as identified by the
FDA. A good working relationship with a seafood supplier
can be the best defense against species substitution. Testing
methods (genetic and electrophoretic) can accurately iden-
tify some species, but these tests are best conducted by con-
tract laboratories.
A second area of economic fraud is the substitution of
imported product for domestic species. The Tariff Act of 1930
mandates that imported seafood be labeled in English with the
country of origin (19CFR134). This is not an FDA requirement
but one of Customs and Border Protection for identification of
the country of origin at the port of entry. The Country of Origin
Labeling (“COOL”) law requiring retailers to identify the origin of
seafood at the point of sale became effective in 2005 (7CFR60). A
third area of economic fraud involves overglazing of seafood. A
glaze is a coating of ice to prevent desiccation. During extended
periods of frozen storage—for example, six months—it is nor-
mal to place a maximum of 18 percent glaze on the seafood
immediately after processing because it sublimates over time.
Fish‐ and Shellfish‐
Derived Foodborne
Illnesses
Ciguatera fish poisoning (CFP) is caused by the consump-
tion of certain predatory fish (grouper, snapper, barracuda,
amberjack, wrasse, surgeon fish, parrot fish, and others) har-
vested from coral reefs in tropical and subtropical waters.35
Smaller species in the reefs consume microscopic marine algae
that contain ciguatoxin, which, through predation, bioaccu-
mulates up the food chain and becomes concentrated in the
larger fish, which are commonly eaten by humans. CFP symp-
toms begin with tingling and numbness of the lips and tongue,
sometimes spreading to the extremities, and also include nau-
sea, vomiting, diarrhea, headache, reversal of sensitivity of hot
and cold, acute sensitivity to temperature extremes, vertigo,
muscular weakness, irregular heartbeat, and reduced blood
pressure.36 Gastrointestinal symptoms usually begin within
two hours of ingestion, and cardio vascular and neurologic
symptoms begin about four hours later. Symptoms can last
c06.indd 93 1/14/2016 7:21:23 AM
94 • CULINOLOGY®
from weeks to months. Ciguatoxin is odorless and tasteless,
heat stable,37 and extremely potent: 0.01 ppb (parts per billion)
for Pacific ciguatoxin equivalents and 0.1 ppb ciguatoxin equiv-
alent for the Caribbean.38
The five types of shellfish poisoning are paralytic (PSP);
diarrhetic (DSP), caused by saxitoxin; neurotoxic (NSP),
caused by brevetoxin; amnesic (ASP), caused by domoic acid;
and azaspiracid (AZP), attributed to azaspiracids. All are
caused by the consumption of molluscan shellfish (clams,
oysters, mussels, scallops), but exceptions exist. Whelk are
also associated with NSP; the viscera of Dungeness and red
rock crabs and roe from scallops are associated with ASP; and
conch, snails, whelk, abalone from South Africa, and the
viscera of crab and lobster are associated with PSP intoxica-
tion.39 Shellfish poisoning is widespread, with outbreaks
occurring mainly in warm waters but also in colder areas
during the summer months. Gastrointestinal effects (nausea,
vomiting, and abdominal pain) are reported with DSP, ASP,
and AZP intoxication, while neurological effects are reported
as a result of NSP, PSP, and ASP intoxication. Onset of symp-
toms is minutes to hours with NSP, DSP, AZP, and PSP, while
ASP onset is 24 to 48 hours. Resolution of symptoms is sev-
eral days. Action levels for each toxin are PSP, 0.8 ppm saxi-
toxin equivalents; NSP, 0.8 ppm brevitoxin‐2 equivalents; DSP,
0.16 ppm as okadaic acid; ASP, 20 ppm domoic acid, except in
the viscera of Dungeness crab, where the action level is 30
ppm; and AZP, 0.16 ppm azaspiracid equivalents.40 Like cigua-
toxin, the causative agents of shellfish poisoning are heat
stable and cannot be destroyed by cooking.
Scombroid poisoning is caused by consumption of certain
species of fish that naturally contain high levels of the amino
acid histidine. Typical species include tuna, mackerel, blue-
fish, bonito, amberjack, bluefish, alewife, escolar, herring,
jack, marlin, sailfish, shad, mahi‐mahi, sardines, and ancho-
vies. If the fish is not kept adequately chilled after capture or
during storage, bacteria multiply and secrete histidine decar-
boxylase, an enzyme that breaks down the histidine to hista-
mine. This is the same histamine that causes allergic
reactions. Symptoms begin within minutes to hours and may
last from 12 hours to several days and include tingling around
the mouth or throat; rash or hives on the upper body; dizzi-
ness; itching; nausea, vomiting, diarrhea; asthmatic‐like con-
striction of the air passage; heart palpitations; and respiratory
distress.41 Histamine is not destroyed by normal cooking
temperatures, so even properly cooked fish can be affected.
Freezing, cooking, smoking, curing, and canning are also inef-
fective in eliminating the toxin.
Escolar (Lepidocybium flavobrunneum) and oilfish (Ruvettus
pretiosus) are two species associated with gempylid fish poi-
soning. These fish produce gempylotoxin, which is a strong
purgative. This is actually an indigestible wax ester that causes
sudden diarrhea. The FDA warns against consumption of
these species. Orange roughy (Hoplostethus atlanticus) is
another species that contains high levels of wax esters capable
of causing sudden diarrhea in susceptible individuals. Food
formulators must be wary of consumer complaints of food-
borne illness after consumption of orange roughy.
Tetrodotoxin is associated with pufferfish (fugu, swellfish,
bok, blowfish, globefish, balloonfish, and sea squab) poisoning.
The United States restricts importation of pufferfish and
products that contain it. The gonads, liver, intestines, and skin
of the pufferfish can contain enough toxin to produce rapid
and violent death. The lethal potency is 5000 to 6000 MU/mg
(1 MU, or mouse unit, is defined as the amount of toxin
required to kill a 20‐gram male mouse within 30 minutes of
intraperitoneal injection), and the minimum lethal dose
(MLD) for humans is estimated to be approximately 10,000
MU (≈2 mg).42 Tetrodotoxin is of major public health concern
primarily in Japan and the Far East, where pufferfish liver (and
FIGURE 6.6 Economic fraud: examples
of species substitution.
Products in Column A have been known to be
substituted for those in Column B, which in most cases
are more expensive (FDA, 2014).
Column A Column B
Various Snappers
(Lutjanus spp.)
Red Snapper
(Lutjanus campechanus)
Rockfish (Sebastes spp.) Red Snapper
(Lutjanus campechanus)
Yellowtail Mahi Mahi
Mako Shark Swordfish
Oreo Dory or John Dory Orange Roughy
Alaska Pollock Cod
Sea Bass Halibut
Arrowtooth Flounder Dover Sole
Black Drum Red Drum (Red Fish;
Southern or Gulf )
White Perch Lake or Yellow Perch
(Great Lakes)
Zander Lake or Yellow Perch
(Great Lakes)
Paddlefish and
other fish roe
Caviar (Sturgeon species)
Sauger Walleye
Pink Salmon Chum Salmon
Skate Wings Scallops
Alaskan Pollock Walleye
Steelhead Trout Salmon
Imported Crabmeat Blue Crabmeat
Farm Raised Salmon Wild Caught Salmon
Top Shell Abalone
Source: U.S. Food and Drug Administration, www.fda.gov (2014).
c06.indd 94 1/14/2016 7:21:23 AM
http://www.fda.gov
CHAPTER 6 • Protein‐Based Foods: Seafood • 95
other parts) were a traditional delicacy until banned in res-
taurants and markets. Pufferfish is prepared and sold in spe-
cial restaurants where trained and licensed chefs carefully
remove the viscera to reduce the danger of poisoning. More
recent research shows that the pufferfish’s diet and enteric
bacteria cause the formation of tetrodotoxin. Mortality from
pufferfish poisoning in Japan was 57.9 percent in 1965 but
reduced to 5.3 percent by 2007. Symptoms of tetrodotoxin
poisoning begin between one‐half and three hours after inges-
tion and include headache and numbness that gradually pro-
gresses to paralysis, irregular heartbeat, respiratory failure,
and death within four to six hours.43 Depending on the dose,
death may occur due to respiratory arrest within two hours of
consumption. There is no antidote. Treatment typically
includes intravenous fluids and respiratory support for
approximately a week in a hospital intensive care unit.
Fresh fish is not always better than frozen fish. For fish
preparations that are normally consumed uncooked, such as
ceviche (which is acid marinated) and sashimi, freezing is
strongly recommended for destruction of parasites. These
are present in some percentage of any catch.
The anasakid roundworm (a nematode) is associated
with saltwater finfish species of the West Coast such as cod,
flounder, halibut, herring, plaice, pollock, and sea bass. These
parasites can cause gastrointestinal discomfort but do not
typically remain viable for more than three weeks in healthy
humans.
Diphyllobothrium tapeworms (cestodes) are associated
with freshwater fish (perch and pike) and anadromous
species (those that migrate from fresh to salt water, such as
salmon).44 Tapeworms can grow to several yards long inside
the human digestive tract if not diagnosed and treated but
can live for months or longer without causing acute illness.
They can successfully be treated with antihelminthic
medications.
Clonorchis sinensis, the Chinese liver fluke, is transmitted
from snails to freshwater fish to humans. This parasite lives
in the liver of humans and is isolated mainly to the common
bile duct and gallbladder, feeding on bile. These organisms,
among the most prevalent worm parasites in the world, are
endemic to Japan, China, Taiwan, and Southeast Asia, where
they infect millions of people. While normally asymptomatic,
most pathological manifestations result from inflammation
and intermittent obstruction of the biliary ducts. The acute
phase consists of abdominal pain with associated nausea and
diarrhea. Recent studies have shown that Clonorchis can
cause cancer of the liver and bile duct. These liver flukes are
extremely rare in the United States.
The traditional way to control for parasites is candling, or
holding thin slices of fish flesh over a bright light to look for
the presence of parasites, followed by their excision. Candling
is estimated to be 70 percent effective (dark tissue or thick
slices are difficult to illuminate from behind), but freezing is
100 percent effective. As a result, the FDA recommends that
seafood to be consumed raw be frozen first. The recommen-
dation is usually freezing solid for 15 hours in a blast freezer
(–31°F [–35°C]) or for one week in a freezer at –20°F (–29°C).
The texture may be somewhat compromised depending on
the freezing temperature, efficiency of the freezer, and the
means of thawing; however, the fish can be served without
fear of harming anyone.
The thawed flesh can then be served raw as sashimi or as
ceviche, where fish is pickled in an acidic fruit juice marinade
that denatures the proteins, firming the flesh and causing its
cooked appearance. Some ceviche restaurants, to be safe,
gently steam the seafood first (until barely cooked), then mix
it with fresh vegetables and citrus juices. Heating to an inter-
nal temperature of 140°F (60°C) or greater—i.e., cooking—
also destroys parasites.
Shellfish
Shellfish Certification
The U.S. Department of Commerce maintains a list of
approved shippers of oysters, clams, mussels, and scallops
(the Interstate Certified Shellfish Shippers List). Only shellfish
from these shippers (whose shellfish come from certified
pathogen‐tested waters) can be sold for consumption.
Wholesale containers must be labeled with the harvester’s
name, address, certification number, date and location of
harvest, and type and quantity of shellfish. Shucked shellfish
(removed from their shells) must also be tagged with a sell‐by
date or a date shucked, and these tags must be kept by food-
service operators for a minimum of 90 days after receipt. Any
violations are reported to the FDA, which has jurisdiction
over shellfish commerce.
Mollusks
Gastropods (Univalves): Abalone, Whelk,
and Conch
The abalone is a gastropod (or univalve). The adductor mus-
cle, or foot, is the predominant protein structure of the aba-
lone. It has a unique muscle structure (Figure 6.8) that allows
the animal to adhere to its habitat, usually a rock, at pres-
sures up to 200 psi. This structure allows for great strength
but requires the muscle to be physically tenderized before
preparation, typically by gently pounding with a mallet.
Abalone was overfished in California by World War II and
hence became only a recreational (sport) fishery. Abalone
fishing in Alaska was closed to commercial fishing in the
early 1980s. Abalone is now aquacultured in California, Japan,
China, Korea, Taiwan, Thailand, Australia, New Zealand, and,
to a lesser extent, in Europe, Iceland, and other Pacific Rim
countries.
The bottom of the foot is the pedal sole, which is not typi-
cally consumed, as it contains up to 22 percent collagen and
is extremely tough.45 Domestically, this part is removed and
discarded in the butchering process. For the export market,
it may be salted and dried for later addition to soups and
stews.
c06.indd 95 1/14/2016 7:21:23 AM
96 • CULINOLOGY®
Some abalone flesh is canned. Due to the presence of
hemocyanin, some blue discoloration may occur over time.
This can be prevented by adding sodium acid pyrophosphate
(SAPP) to the pack before the thermal process.
Abalone is one of the most expensive seafoods in the mar-
ketplace. Fresh abalone steaks are available on Amazon.com
for $125.00/pound (plus shipping). Not surprisingly, the FDA
has observed illegal species substitution with gastropods
from the Trochidae family (whelks).
Both the conch and the whelk are univalves, like abalone,
and must be similarly tenderized because the meat is rather
tough in its native state. The flavor is clam‐like. Preparation
time is short, and overcooking causes toughening. Whelk is
commonly used in Italian cooking, where it is known as
scungilli. Conch (pronounced “konk”) is famous in the
Caribbean for use in fritters. Due to overharvesting, the
United States has neither a commercial nor a recreational
fishery for queen conch. There is a controlled fishery for
queen conch in the Caribbean.
Whelk of the Neptunia species have a toxin, teramine, in
their salivary glands. Symptoms of teramine poisoning include
double vision, temporary blindness, difficulty focusing, tingling
of the fingers, vomiting, diarrhea, and loss of muscle control.
Symptoms develop within one hour of consumption.46
FIGURE 6.7 Evaluating live and fresh shellfish.
Select for Live Shellfish Avoid
Firmly closed shells or gaping shells
that close firmly when tapped
Oysters and clams Gaping or open shells that do not
close when tapped; the animal is dead
and inedible.
Animals that seem heavy for their size
and have leg movement
Hard and soft shell crabs
and lobster
Sluggish or inanimate animals are
either dying or dead and are inedible.
Note: Animals should never be
shipped in dry ice.
A tail that curves under the animal
when picked up
Lobster A tail that hangs limply when the
animal is picked up
Fresh Shellfish
Shucked meats should be plump with
no more than 10% liquor that is creamy
in color and clear.
Oysters Meats that appear to be misshapen
and dispersed in turbid liquor and/or
an overabundance of liquor
Shucked meats will be yellow to pink
in a clear liquor.
Clams Orange to brown meats in turbid
liquor
Scallops are firm with a slightly sweet
aroma and have very little liquor; sea
scallops are white to pink to orange;
bay scallops are smaller with white,
tan, or pink coloration. (Note: Color is
primarily due to dietary carotenoids.)
Scallops Soft or mushy meats; strong, putrid, or
ammonia‐like odors; excessive fluid in
the package
- Slightly softer than other species of
shrimp; easier to peel
- Natural sweet flavor; seaweed‐like
aroma
- White to grayish‐pink
- Cooked flesh has pink color
White shrimp Pitted or bleached shells (over-
sulfited); intense pink raw flesh
indicates temperature abuse; strong
or chemical odors; frozen, clumped
shrimp indicate previously frozen
product.
- Slightly firmer than white U.S. Gulf
shrimp
- Larger sizes (10/15) may have a
stronger iodine flavor and more
intense brown coloration
- Tend to be rounder than white shrimp
so counts may appear skewed
- Have a groove in the last tail segment
Brown shrimp Soft or mushy tails; strong or ammo-
nia‐like odors; black spot (melanosis)
caused by a natural enzymatic process
post‐harvest (which can be inhibited
by brief dips in sodium metabisulfite or
Everfresh [4‐hexylresorcinol on a NaCl
carrier])
Source: Sea Grant College Program.
c06.indd 96 1/14/2016 7:21:23 AM
CHAPTER 6 • Protein‐Based Foods: Seafood • 97
Bivalves: Clams
Clams are mollusks with two shells (bivalves). Clams can be
eaten raw, steamed, boiled, baked, or fried. The method of
preparation depends partly on the size and species of the
clam. They can also be made into clam chowder (a popular
soup in the United States and Canada), or they can be cooked
using hot rocks and seaweed in a New England clambake.
The two general categories of clams are hard shell and soft
shell. The hard‐shell clam (Mercenaria mercenaria) is also
known as the Northern quahog, cherrystone clam, and little-
neck clam. It is harvested either wild or farmed from the Gulf
of St. Lawrence to the Gulf coast of Texas. The ocean (or
deep‐water) quahog or mahogany clam (Artica islandica) is
dredged from deeper waters of the North Atlantic and
brought to shore in large metal cages.47 Once ashore, they are
offloaded to refrigerated areas. Typically, they are conveyed
through a flame or steam or water bath to open the shell. The
meats may then be hand shucked (or mechanically eviscer-
ated by shakers); the viscera, or sandbag, is then removed and
discarded. The cleaned meat is washed and cut or diced and
then packed for refrigerated distribution, freezing, or canning
(retorting). Clams may also be sold live for cooking or for raw
consumption. Other hard‐shell clams of economic impor-
tance include the surf or bar clam (Spisula solidissima) from
the Gulf of St. Lawrence to Hatteras, North Carolina, and the
Pacific razor clam (Siliqua patula), with shells shaped like an
old‐fashioned straight razor; these are harvested from south-
ern California to the Aleutian Islands of Alaska. Protothaca
tapes, the littleneck clam, and Saxidomus giganeus or S. nut-
talli, the butter clam, are two other hard‐shell clams har-
vested off the Pacific coast.
The other major category of clam is the soft‐shell or steamer
clam. Soft‐shell (long‐neck) clams or steamers (Mya arenaria)
occur naturally from Labrador, Canada, to North Carolina and
were introduced on the West Coast. They are dug and sold live
for the traditional clambake or steaming, or they may be
shucked and preserved for stews and chowders. They are char-
acterized by an elongated and flattened shell that is much
thinner and more brittle than hard‐shell clamshells and more
easily broken, hence the term soft‐shell clams. They are also
characterized by a long siphon from which they eject seawater,
which has given them the colorful nickname “piss clam.”
The largest edible clam is the geoduck (Panopea generosa),
pronounced “gooey‐duck.” This West Coast clam has a large
shell (7 to 9 inches [17 to 23 cm]) and an unusually long
siphon (typically 2 to 3 feet [0.6 to 0.9 m]), as it buries itself to
the depth of the siphon below the surface of the beach.48
Typical harvest weights are in the 2‐pound (0.9 kg) range. It is
also one of the longest‐living animals on Earth, with a known
lifespan in excess of 100 years. The market for these clams is
primarily in Asia, where they are highly valued. As such, these
clams are actively farmed, especially in Washington State’s
Puget Sound. Geoduck is often seen at the Pike Street Market
in Seattle and sold live to specialty restaurants.
A note on color: Green gills on clams and oysters may result
from diet. The single‐cell alga Haslea ostrearia (a diatom)
FIGURE 6.8 Abalone structure. The microstructure of abalone (Haliotis rufescens) is shown. The
transverse fibrils show the strength of the adductor and the need for mechanical tenderization
before consumption.
Source: Lampila, unpublished data.
c06.indd 97 1/14/2016 7:21:26 AM
98 • CULINOLOGY®
produces the water‐soluble blue pigment marennine.
Marennine deposits in the gills after filter feeding and as it con-
centrates imparts a green color to the natural yellowish color
of the organs. This diatom tends to flourish in colder weather
but varies from one body of water to another. Marennine is not
toxic to humans but in the United States is considered a sen-
sory defect. In France, however, oysters that have bioaccumu-
lated marennine are considered a delicacy. There, the diatoms
are actually cultivated to be released into oyster “finishing
beds” (clairs) to encourage the development of green gills.
Yellow to orange colors are imparted by dietary carot enoids
from phytoplankton, which are estimated to produce several
hundred tons of carotenoid each year. Clams, like humans,
must derive carotenoids from the diet, as they cannot be syn-
thesized. Carotenoids are normally considered fat soluble.
When bound to hydrophobic sites on proteins, they can become
soluble in an aqueous medium and are termed carot enoproteins.
Carotenoproteins primarily occur in invertebrates.
The forms of carotenoid seen most often in clams include
β‐carotene (yellow to orange in color and most bioactive pre-
cursor to vitamin A), zeaxanthin (yellow), and lutein (yellow).
The functions of the carotenoids in mollusks are not known
but may include strengthening the protein structure; as anti-
oxidants to quench singlet oxygen (free radicals); imparting
resistance to bacterial or fungal diseases; enhancing immune
function; promoting wound healing; and providing protec-
tion from light. They are known to take part in the develop-
ment of reproductive organs.49
Pink to red colorations can be caused by the reduction of
nitrate deposited by certain strains of sulfur‐oxidizing bacteria
living within the gills of the host clam. Elevated levels of nitrite
postmortem can lead to pinking of the natural meat color.
Residual nitrate (NO
3
−) can also be reduced to nitrite (NO
2
− and
nitric oxide (NO) by microbial decomposition. This is analo-
gous to the reactions that create the permanent pink color in
cured meats.
myoglobin + nitric oxide → nitric oxide myoglobin →
Δ → nitrosyl hemochrome (pink color)
Black discolorations (spots to streaks) can be imparted
by hydrogen sulfide binding to essential iron‐containing
compounds such as hemoglobin (which carries oxygen) and
cytochromes (the compounds responsible for aerobic respi-
ration). Some sulfur‐oxidizing bacteria can concentrate sul-
fur and form filaments; this is a mechanism of energy storage,
and more prevalent in cultured than in wild‐caught clams.
While not harmful, it is probably best to not use clams with
blackening, as the consumer would probably deem these
spoiled.
Bivalves: Mussels
Blue mussels (Mytilus edulis) are harvested from the waters
of the North Atlantic. They are sold live, and normally the
whole animal in the shell is steamed before serving. Mussels
may be commercially steam cooked and the meats removed
and frozen for later addition to soups, stews, and chowders.
At –4°F (–20°C), storage time is estimated to be nine months
without flavor deterioration; however, shelf life is reduced to
approximately three months at 19°F (–7°C). The New Zealand
green‐lipped mussel (Perna canaliculus) has recently received
a great deal of attention due to reports that it possesses anti‐
inflammatory (anti‐arthritic) compounds. For the formula-
tor, the flavor of mussels is the most intense of the bivalves;
they can be used in highly seasoned tomato or other sauces,
where the flavor of oysters and clams would be masked.
Bivalves: Oysters
Three primary oyster species are harvested in the United
States: Crassostrea gigas, or the West Coast oyster, trans-
planted from Japan and maricultured (a type of aquaculture
where the farming takes place in sea water); Crassostrea vir-
ginica, or Eastern variety, native to the waters of the mid‐
Atlantic; and the Olympia oyster (Ostrea lurida), indigenous to
the Pacific Northwest. There is also a limited fishery of the
European oyster (Ostrea edulis) off the Maine and Gulf coasts.50
C. gigas was imported to the Northwest from Japan after
the indigenous species (Ostrea lurida) was overfished, com-
promised by other environmental factors, and subsequently
failed to thrive. This is a large, meaty oyster whose slightly
salty flavor is distinctive to its growing waters. C. virginica, a
smaller cousin, is said to have a sweeter flavor reflective of
Eastern growing waters. The Olympia oyster is the smallest
(about the size of a half‐dollar), and its culture is limited to a
modest area (700 acres) in Washington State.
All three primary oyster types can be consumed raw,
directly out of the shell. The two larger species may be
shucked and sold in that form in their liquor or juices. The
FDA standard of identity for fresh shucked oysters allows not
more than 5 percent weight loss when a representative gallon
of oysters is drained for 2 minutes in a sieve within 15 min-
utes of packing. Furthermore, no added food ingredients are
permitted.
Shucked oysters have a unique pH profile during spoilage
because pH continues to decrease with time. This is directly
related to the naturally high glycogen (stored carbohydrate)
content of the oyster, which continues to degrade to lactic
acid postmortem. Glycogen content is highest in May and
lowest in August. After shucking, the pH ranges from 5.9 to
6.2; at pH 5.8, the odor is considered off; at pH 5.7 to 5.5, it is
characterized as musty, and at pH 5.2 or less, it is putrid or
sour.51
Pathogens can be of concern with the consumption of raw
oysters. Waters are routinely monitored for viruses and fecal
indicators and, when present, the relevant government entity
closes the harvesting beds. The bacterium of greatest concern
is Vibrio vulnificus, which is seen only in warm waters; con-
sumption of it can be fatal to susceptible populations. Those
most susceptible are individuals with hemochromatosis
c06.indd 98 1/14/2016 7:21:26 AM
CHAPTER 6 • Protein‐Based Foods: Seafood • 99
(a condition where excess iron is carried in the blood);
hypochlorohydria (low stomach acid) as a result of genetics or
partial gastrectomy (partial stomach loss due to accident or
disease); cirrhosis of the liver (especially middle‐aged men) or
immunocompromised individuals (people who have AIDS,
who are receiving cancer treatments, etc.). Vibrio vulnificus is
closely related to Vibrio cholerae, the microbe responsible for
cholera.
Regulation of oysters is voluntary (with essentially 100
percent compliance), sponsored by the Interstate Shellfish
Sanitation Conference; its protocol is called the National
Shellfish Sanitation Program (NSSP). The NSSP has an initia-
tive to prevent foodborne infection via V. vulnificus. The state
of California mandated that beginning in 2003, during the
warmer summer months, states bordering the Gulf of Mexico
could sell in‐shell oysters only if they are processed through a
post‐harvest V. vulnificus destruction step. This may be high‐
pressure processing (HPP), low‐temperature pasteurization,
cryogenic freezing, or irradiation. Each of these processes is
currently in commercial application. The only process that
results in a live oyster is irradiation; however, the animal lives
just seven to nine days after exposure.
Bivalves: Scallops
Scallops are harvested and shucked on board the fishing ves-
sel for the adductor muscle that holds the two shells together.
The body of the scallop is not normally eaten. The color of the
scallop varies with its diet and may be ivory, tan, light yellow,
pink, or a light coral. The two major types are ocean and bay
scallops (large and small, respectively). Occasionally, a third
type of intermediate size, the calico scallop, is seen.52 For the
most part, scallops are hand shucked on smaller vessels;
however, on larger factory vessels, the scallops may be steam
shucked. This steaming process often causes a case harden-
ing (surface denaturation due to heat), and the scallops may
have a slightly different appearance. To flavor steam‐shucked
scallops with a marinade, vacuum tumbling is recommended
to encourage absorption of the marinade.
Scallops can be vacuum tumbled to promote penetration
and distribution of the marinade. All massaging or tumbling
should be conducted under refrigeration <41°F (5°C). Never
tumble in an excess of marinade containing salt or alkaline
phosphate, which causes extraction of functional (water‐
binding) protein because any liquid that is not absorbed will
be discarded when the tumbler is drained. This will result in
tough, dry scallops. Under 50 percent vacuum, smaller
pieces must be tumbled for 30 to 45 minutes. Phosphate‐
containing blends accelerate uptake of marinades so tum-
bling time can be reduced. Larger pieces may require longer
tumbling. Rotation of the tumbler should be gentle—6 to 8
rpm—to keep the scallops intact. The seafood may be imme-
diately cooked, vacuum packaged and frozen, or atmospher-
ically packaged and stored at refrigerated temperature for
wholesale or retail use.
Other species similar to scallops that have a hearty or
robust structure (firm, dense flesh; dense myocommata;
resistance to gaping), such as catfish and shrimp, can also be
vacuum tumbled. The structure of tuna and salmon does not
lend itself to tumbling (but can withstand spice rubs or 10‐ to
15‐minute dips in marinades).
Scallops are prone to economic fraud due to species sub-
stitution. The muscle bundles should run longitudinally in a
scallop. Skate or ray wings are sometimes substituted and
can be identified by the horizontal orientation of muscle bun-
dles and a difference in aroma, as the substituted species is
an elasmobranch (shark family) that contains abundant urea
that hydrolyzes to ammonia. This entrepreneurial effort is
illegal; it arises approximately every 15 years and is quickly
responded to by the FDA.
Cephalopods: Squid, Octopus,
and Cuttlefish
Squid, cuttlefish, and octopus are members of the molluscan
class Cephalopoda (from the Greek for “head‐footed”).
Cephalopods are among the smallest classes of seafood har-
vested (together with mollusks, about 3 percent of the total
seafood harvest).
Squid is prized for its mantle (body) and tentacles. A
popular means of preparing squid is by slicing, battering,
breading, and deep‐frying these parts as calamari. The mus-
cle structure is similar to abalone in that the layers of hori-
zontal fibers overlay shorter perpendicular fibers. Squid
salt‐soluble (functional) protein has an isoelectric point
lower than that of finfish and swells in dilute salt solutions
to a greater extent. Squid also has an unusual water‐soluble
actomyosin (contractile protein).53 Fresh meat should be
white; with age, freezing, and alkaline treatments, it red-
dens. Purple colorations result from pigment in the
chromatophores: tiny sacs of pigment in the skin that allow
the squid to change color at will. These provide the squid
with instantaneous camouflage that in life act as a defense
mechanism.
Cuttlefish (Sepia officinalis and S. orientalis) are widely
used in Asian and Italian cooking as an inexpensive replace-
ment for squid, which has similar flavor and texture profiles.
Cuttlefish, squid, and octopus are also preserved by drying
and are consumed that way as a snack.
Octopus (Octopus vulgaris) is commonly harvested off
the East and West coasts of the United States. It has been
fished for many years to be used as bait for halibut. Octopus
is popular in many Spanish, Italian, French, Latin American,
and Asian cuisines, where the tentacles and body sac are
stewed or braised and occasionally grilled. It has become
more commonly consumed in the United States as ethnic
cooking has become mainstream. It is also eaten raw as
sashimi. Large octopi must be tenderized in a manner sim-
ilar to abalone. Octopus contains a high level of moisture
c06.indd 99 1/14/2016 7:21:26 AM
100 • CULINOLOGY®
(76 to 82 percent) and between 0.25 to 0.50 percent lipid.
The lipids are composed of about 55 percent polyunsatu-
rated fatty acids, 30 percent saturated fatty acids, and 10
percent monounsaturated fatty acids.54 Due to the high
percentage of polyunsaturates, octopus must be kept iced
and, once cleaned, packaged to exclude oxygen to prevent
rancidity.
Crustaceans
The pH of crustaceans (lobsters, crabs, shrimp, crawfish,
etc.) tends to run higher than finfish and mollusks—between
6.8 and 8.2, with the higher end of the scale occurring at or
near the time of molting (shedding the shell and growing a
new and larger shell). This is a direct cause of excess calcium
carbonate (an alkaline substance) that is briefly free‐
circulating in the animal in preparation for formation of the
new shell.
Crabs
Unlike other animals, most other crustaceans, and mollusks
(but similar to abalone), crabs have a blood protein called
hemocyanin in which copper is the oxygen acceptor rather
than the iron in the hemoglobin contained in red blood cells.
Postmortem, especially in pasteurized flesh, the copper may
bind with phenolic groups present in the structure of the
amino acid tyrosine and cause a blue discoloration. The blu-
ing can be prevented by adding a sequestrant (typically
sodium acid pyropyhosphate at 0.25 to 0.5 percent of the
meat), which binds the copper to prevent this reaction.
Note: Sequestrants are food additives, also known as
chelating agents, used to bind to and literally sequester
metals—keeping them away from other compounds found
naturally occurring in seafoods (and other foods) that the
metals can react with and cause assorted problems during
processing and storage. In addition to preventing bluing in
canned shellfish and crustacea, sequestrants can prevent the
formation of “black spot” in shrimp stored on ice, and struvite
crystal formation in various canned seafood products.
(Struvite crystals are harmless crystals of magnesium ammo-
nium phosphate resembling glass, which can form following
retorting and continue to “grow” during storage.) Sequestrants
are also effective in preventing rancidity in oily fish such as
salmon fillets.
Blue crabs (Callinectes sapidus) are harvested from waters
on the East Coast of the United States and the Gulf states.
Live crabs are typically steam cooked under 12 to 15 pounds
of pressure (in some states of the mid‐Atlantic) or boiled,
cooled, and picked (Gulf region). The yield is 10 to 12 percent
for the typical blue crab and up to 14 percent for a large crab.
As these crabs are cooked live, their form of blood protein,
hemocyanin, remains within the edible flesh. Soft‐shell crab is
a culinary term for blue crabs that have recently molted their
old exoskeleton; they are consumed while the new shell is
still soft. The shell is left intact when soft‐shell crabs are
prepared for cooking and eating, as it is entirely edible. They
are most typically prepared by lightly dredging in seasoned
flour and sautéing.
Stone crabs (Menippe spp.) are similar to blue crabs, but
their front claws grow larger and are the only part that is con-
sumed. These claws can regenerate several times, and while
it is legal to remove both claws before releasing the crab, mor-
tality rates are twice as high for doubly amputated crabs as
for single amputees. Claws must be at least 2¾ inches
(7 cm) long from the tips of the immovable finger to the first
joint, and it is illegal to harvest the whole animal. Although
they exist along the coast of the Gulf of Mexico, most are har-
vested for commerce from Florida waters.55
Dungeness crab (Cancer magister) is harvested off the
West Coast from California to Alaska. They may be cooked
and picked immediately or “backed” (butchered with viscera
removed but legs remaining attached to each half of the
frame) and brine‐frozen raw for later cooking and picking.
Unlike blue crab, Dungeness is either steamed or immersion
boiled without pressure. The shells of Dungeness crab are
extremely hard and must be broken for picking with the aid of
a small mallet or nutcracker. Yield of Dungeness crab may be
as high as 55 percent.
Alaska king crab (Paralithoides spp.) is the largest domes-
tic crab, with an average weight of 12 pounds (5.5 kg). The
carapace (shell covering the back) is small (7 to 9 inches [17
to 23 cm]) relative to the legs (easily 3 to 4 feet [0.9 to 1.2 m]),
where the edible flesh is located. After cooking, the meat
falls easily from the shell, making this one of the easier crabs
to pick. The yield of the legs averages 55 to 60 percent cooked
meat.56 Snow or tanner crab (Chionecetes spp.) is a smaller,
2½‐ to 3‐pound (1.13 to 1.36 kg) version of the Alaska king
crab. The yield of cooked meat from the legs is similar
as well.
Lobsters
Three types of lobsters are commercially fished in the United
States. The Northern or cold‐water lobster (Homerus ameri-
canus) is fished from Newfoundland, Canada, to Maine; the
Southern or warm‐water lobster (also known as the spiny or
rock lobster, Panulirus argus); and the California spiny lobster
(Panulirus interruptus). The Northern lobster has the distin-
guishing large front claws and substantial tail from which
edible flesh is removed, while the Panulirus spp have no
claws and yield meat only from the tail. Legal size of harvest
for the Southern lobster is a 3‐inch (7.6 cm) carapace; for the
California, a 3¼‐inch (8.3 cm) carapace; and for the Northern,
a carapace between 3¼ and 5 inches (8.3 to 12.7 cm) in
length.
Langostinos are similar to lobsters, although they are actu-
ally related to hermit and porcelain crabs. Lobsters have larger
tails than langostinos (Pleuroncodes monodon, Cervimunida
jolni, Pleuroncodes planipes, Munida gregaria), whose short tail
is perfect for bites, or restructured lobster products and mix-
tures containing meat pieces.57 The flavors of lobster and
c06.indd 100 1/14/2016 7:21:26 AM
CHAPTER 6 • Protein‐Based Foods: Seafood • 101
langostinos are similarly sweet and delicate, but the texture of
the langostino is closer to shrimp than to lobster. The FDA
requires that langostino not be marketed as lobster; if lobster
is in the name of the product, the word langostino must pre-
cede the word lobster. (Due to its firm texture and somewhat
similar flavor, monkfish is also sometimes substituted; it is
referred to as poor man’s lobster.)
Lobsters are immersion cooked in either plain water or
salt water (containing 3 to 5 percent salt), cooled, and hand
picked in commercial plants. Struvite crystals (magnesium
ammonium phosphate) resembling shards of glass may form
in lobster flesh cooked in seawater (due to the presence of
magnesium) if not consumed immediately. Lobsters are also
shipped live but should never be shipped with dry ice as the
carbon dioxide gas causes asphyxiation. Soft‐shelled Northern
lobsters (similar to soft‐shelled crabs) exist but do not gener-
ally survive being shipped long distances.
Shrimp
Several species of shrimp are harvested from domestic waters,
but these constitute only about 10 percent of all shrimp
consumed in the United States, most of which is aquacultured
product imported from Thailand, Indonesia, Ecuador, China,
India, the Philippines, Mexico, and other countries. The edible
flesh of shrimp is located in the tail. The yield of edible flesh
from domestic shrimp is just over 60 percent. In the southern
United States, it is common to cook and serve whole shrimp,
head‐on and unpeeled, to enhance flavor.
Warm‐water shrimp from the Gulf of Mexico—white
(Penaeus setiferus), brown (Penaeus aztecus), and pink (Penaeus
duorarum)—are among those wild caught.59 Cold‐water
shrimp on the West Coast include Pandalus jordani (high
counts per pound), and off the Northeast coast, Pandalus bore-
alis.60 White shrimp are also caught off the Southeast coast.
P. vannemei is aquacultured in North Carolina and Texas.
Warm‐water shrimp can be wild caught to large sizes:
P. aztecus in 2009 was reaching counts of 13 per pound. This is
significant because the larger a brown shrimp is, the more iodine
flavor it exhibits. The preferred wild Gulf shrimp flavor (sweet
with a hint of iodine) has been analyzed and compared with that
of the aquacultured imported product. The flavor differs with
bromophenol content61 (bromophenols are natural chemicals
that occur in small amounts in the muscle tissue of wild shrimp),
which reflects the specific diet of the wild shrimp. Efforts to sim-
ulate the wild diet in aquacultured shrimp have been thus far
unsuccessful. Despite the significant difference in the flavor of
wild Gulf shrimp, geographic source indicators on packaging
to identify better‐tasting shrimp have not yet been used to
generate premium pricing. A strong education effort is needed.
Market forms of shrimp are: cooked and peeled (especially
P. jordani); in the round (whole, head on); green headless
(green indicates unprocessed shrimp, not its color); peeled
and deveined (P&D); and peeled and undeveined (PUD).
Products may be sold in individual quick‐frozen (IQF) forms
in poly bags or in blocks ranging from 1 to 5 pounds (0.45 to
2.3 kg) each in 20‐pound (9.1 kg) master cartons. One pound
(0.45 kg) head‐on shrimp yields 0.65 pounds (0.3 kg) of white
shrimp meat and slightly less of pink and brown shrimp meat.
Well‐handled and chilled, fresh, headless shrimp have a shelf
life of 10 to 12 days; head‐on product has significantly shorter
shelf life due to the presence of the hepatopancreas in the
head, which contains an extremely high concentration of
digestive enzymes and bacteria. (The hepatopancreas is the
primary digestive organ of shrimp. It has the dual role of
secreting digestive enzymes and absorbing nutrients.)
Shrimp are sold by count per pound, typically green and
headless. Historically, the ratios run around 21/30, 31/40,
41/50, etc. Increasingly, imported shrimp are sold within nar-
rower ranges such as 31/35, 36/40, or similar. This is in
response to wholesalers desiring greater size consistency and
uniformity, especially in volume feeding outlets.
All shrimp (and lobster) are susceptible to melanosis, or
black spot. This is caused by the enzyme polyphenoloxidase
(also called phenolase, oxidase, or tyrosinase), the same
enzyme that causes browning in cut fruits and vegetables
such as bananas, avocados, apples, and potatoes. It causes
harmless but unappetizing black spots to form along the tail
of the shrimp. This visual defect can be inhibited by dipping
the shrimp in a solution of either sodium metabisulfite or
Everfresh® (4‐hexylresorcinol on a salt carrier). Some indi-
viduals, especially asthmatics, are sensitive to the presence of
sulfite; therefore, if metabisulfite is used, the FDA requires
labeling when levels exceed 10 ppm.
FIGURE 6.9 Shrimp counts. Count range
per pound of shrimp.
Count per
Pound Size
Name
Green
Headless
Number of
Shrimp per
Pound
(over)
Number of
Shrimp per
Pound
(not over)
Extra Colossal Under 10 0 9.9
Colossal 10/15 9.9 15.0
Extra Jumbo 16/20 15.0 20.0
Jumbo 21/25 20.0 25.0
Extra Large 26/30 25.0 30.0
Large 31/35 30.0 35.0
Medium
Large
36/42 35.0 42.0
Medium 43/50 42.0 50.0
Small 51/60 50.0 60.0
Extra Small 61/70 60.0 70.0
Tiny >70 70 —
Source: Idyll, 1976.58
c06.indd 101 1/14/2016 7:21:26 AM
102 • CULINOLOGY®
Crawfish
Crawfish (Procambarus clarkii [red swamp crawfish] and
P. zongangulus [white river crawfish]) are also referred to as
crayfish, mud bugs, and crawdads. A blue crawfish also exists,
but this is prized in private aquariums. Domestic production
of these crustaceans is in the Atchafalaya Basin of Louisiana.
Availability of fresh product is greatest in March, April, and
May. Crawfish are also wild caught in Louisiana and southern
Mississippi. Annual production is subject to boom and bust
years. Crawfish are boiled, the tail peeled, frozen, and sold in
1‐pound (0.45 kg) blocks in poly bags.
Yield is greatest (to 20 percent) among medium‐size ani-
mals and lowest (8 percent) among larger animals, as devel-
opment of the tail is sacrificed as the head and claws grow.
For crawfish boils (with seasoning, corn, potatoes, and other
desired ingredients), larger animals are preferred.
Historically, it was thought that the tails of crawfish must
curl when cooked. A straight tail was thought to indicate an
animal that was dead when boiled. Research shows that the
straight tail is caused by overcrowding in the boiling vessel,
allowing inadequate room for the tail to curl.
Other Seafood
Alligator (Alligator mississippiensis) is a reptile that spends
much of its life in the water. The primary source of meat is
from farmed animals. Contrary to popular belief, alligator is not
an endangered species. In fact, meat from wild animals is
legally marketed in September, the official alligator‐hunting
season.
The flesh is white to light red, and the texture is chewy.
The flavor is mild if the meat is fresh and well handled, but
it can vary according to diet. Protein content is around
22 percent, and total lipid ranges from 1 to 3 percent depend-
ing on location in the body.62 Fatty acid content is highly
unsaturated if the diet is high in fish of marine origin but
more saturated if beef is a staple. The diet depends on the
grower.
Nutritional Value
of Seafood
Protein Quantity and Quality
Total protein can vary widely in wild species from 7 to 24
percent. Mollusks, such as oysters and clams, tend to con-
tain less protein, while albacore, halibut, and skipjack tuna
tend to contain more. Digestibility of seafood protein typi-
cally exceeds 90 percent, with a high PER and Amino Acid
Score (the ratio of 1 gram of the limiting amino acid in a test
diet to the same amount of the corresponding amino acid in
a reference diet).63 All seafood protein has a full complement
of the essential amino acids and is therefore considered
complete protein.
As early as the first quarter of the twentieth century,
digestibility of seafood protein was under evaluation. Boiled
fish was shown to be more readily used than beef.64 Katsch,
using human stomachs, reported greater gastric secretion to
fish than to beef, leading to speedier digestion. Some process-
ing, smoking in particular, was shown to slow the digestion of
fish to nearly the level of beef. 65
Digestibility (the relative ease of digesting a food com-
pared to one with a perfect value of 1.00) of fish typically
ranges between 0.78 and 0.89,66 and Acton and Rudd
estimated digestibility to range between 0.90 and 0.99, while
beef and chicken range from 0.87 to 0.90.67 Egg white, casein
(milk protein), whey protein, and soy protein have scores of
1.00. White bread is reported to be 0.87 and whole wheat
bread 0.68.68
Terrestrial animals tend to contain higher concentrations
of saturated fat, which digests more slowly than the polyun-
saturated oils of seafood, and this extends the feeling of full-
ness or satiety. Second, as animals age, especially cattle and
sheep, collagen becomes more highly cross‐linked, and it
takes longer to hydrolyze during the digestive processes.
Historically, it was thought that myocommata did not cross‐
link, but this was disproved by Bremner, who demonstrated
cross‐linking in some fish species (for example, blue grena-
dier).69 Cross‐linking slows digestibility of the protein. The
third factor that may allow for high and rapid digestibility of
seafood is the shortness of the muscle fiber in fish as com-
pared with terrestrial animals.
Fat Content
In general, many seafood species (halibut, offshore tuna, lob-
ster, shrimp, crab, crawfish, clams, and oysters) are low in
total fat, certainly lower than in equivalent amounts of ter-
restrial proteins, including chicken.
Unlike terrestrial animal fats, up to 30 percent of the fatty
acids contained in some seafood are the highly unsaturated
omega‐3 fatty acids. This includes docosohexaenoic acid
(DHA) and eicosapentenoic acid (EPA). Some fish (wild‐
caught salmon, mackerel, herring, locally caught tuna, and
anchovy) are excellent sources of omega‐3 fatty acids, which
are important in decreasing the risk of heart disease and
inflammatory diseases such as rheumatoid arthritis, and are
a building block of brain and retinal tissues. It is recom-
mended that two to three meals each week have wild‐caught
seafood as the center of the plate. Saturated fatty acids com-
monly isolated from terrestrial animals are replaced in most
seafood by monounsaturated fatty acids such as oleic acid,
which is common in avocados and olives. The highest levels
of monounsaturated fatty acids are observed in wild black
cod (sablefish) and spiny dog fish. The fatty acid content of
aquacultured seafood depends on its diet.
With the exception of the fattier species (mackerel,
herring, salmon), most fish contain <120 calories per
3‐ounce (85 g) cooked serving. The fattier fish are closer to
c06.indd 102 1/14/2016 7:21:26 AM
CHAPTER 6 • Protein‐Based Foods: Seafood • 103
175 calories per 3‐ounce serving. A 3‐ounce (85 g) serving of
steamed or boiled wild‐caught Louisiana shrimp is less than
100 calories.
Although relatively low in fat, some shellfish, especially
shrimp, contain levels of cholesterol nearing 150 mg per
standard 3‐ounce (85 g) serving. The cholesterol content is
outweighed by the other health benefits (high polyunsatu-
rated fatty acids [PUFAs] and lean protein) that eating sea-
food provides.
When calculating the caloric content, multiply grams of
protein and carbohydrate each by 4 (4 kilocalories per gram)
and the grams of fat by 9 (9 kilocalories per gram). When cal-
culating the macronutrient content, it is important to con-
sider proximate composition. This is: the content of total
moisture (water) + protein + lipid (fat or oil) + ash (the inor-
ganic residue after incinerating biologic material) 70 + carbo-
hydrate = 100 percent. For muscle foods, the equation is
usually [100 – (moisture + protein + fat + ash)] = carbohy-
drate, as total carbohydrate is typically negligible and the
most difficult component to assay.
Mineral Content
Saltwater fish contribute iodine, which prevents goiter
(extreme swelling of the thyroid gland due to iodine defi-
ciency), and selenium, which is associated with reducing tox-
icity of organic mercury (methyl mercury or MeHg).71 The
bones in canned salmon, mackerel, and sardine provide a
measurable source of calcium and phosphorus.
Mercury
Inorganic mercury occurs naturally in the environment.
However, a great deal makes its way into the waterways
from smelting, the combustion of fossil fuels, and the man-
ufacture of electronics and amalgam (used for dental fill-
ings). Once consumed, it is methylated by bacteria in the
gut of fish. Methylmercury bioaccumulates in fish as
smaller species are consumed by larger predators. Human
exposure to methylmercury occurs when the larger preda-
tors, such as tuna and swordfish, are consumed. Methyl-
mercury leads to neural defects in children, expressed as
impaired behavioral and cognitive development. The fetus
and young children are at greatest risk for developmental
delay through exposure to methylmercury, as it can cross
both the placenta and the blood‐brain barrier. Elevated
prenatal exposure to methylmercury is associated with
mental retardation, limb deformity, poor growth, and sen-
sory impairment.72 The FDA recommends that women of
childbearing age and young children avoid consuming
shark, swordfish, tilefish, and king mackerel. In order to
partake of the health benefits of seafood, the FDA recom-
mends eating two meals or 12 ounces each week of shrimp,
canned light‐meat tuna (not albacore or white‐meat tuna),
salmon, pollock, and catfish.
Conclusion
As the public becomes more aware of the myriad healthful
benefits of increasing the amount of seafood in the diet,
Culinology® professionals are expected not just to identify
the various species but also to become more proficient
in using them creatively to develop restaurant‐quality
applications that are safe, delicious, and economical to
produce.
Acknowledgments
The senior author wishes to acknowledge Tony’s Seafood and
Louisiana Fish Fry of Baton Rouge, Louisiana, for their gener-
osity in permitting free access to their facility to photograph
seafood species and their products. Also acknowledged for
her professional photography and artistic ability is Paula
Ouder, photographer for the Louisiana Sea Grant College
Program.
1K. A. Lellis‐Dibble, K. E. McGlynn, and T. E. Big ford, “Estuarine Fish
and Shellfish Species in U.S. Commercial and Recreational Fisheries:
Economic Value as an Incentive to Protect and Restore Estuarine
Habitat,” NOAA Technical Memorandum NMFS‐F/SPO‐90 (2008), Silver
Spring, MD, 102.
2About Seafood.com, accessed August 27, 2013, http://www.aboutseafood
.com/about/about‐seafood/top‐10‐consumed‐seafoods.
3M. V. Lebour, “The Larval and Post-Larval Stages of the Pilchard, Sprat and
Herring from Plymouth District,” Journal of the Marine Biological Association
of the United Kingdom (New Series), 12, no. 3 (1921): 427–457.
4Z. E. Sikorski, A. Kolakowska, and B. S. Pan, “The Nutritive Composition of
the Major Groups of Marine Food Organisms,” in Seafood: Resources,
Nutritional Composition, and Preservation, ed. Z. E. Sikorski (Boca Raton,
FL: CRC Press, 1990).
5A. Brown, “Composition of Fish: Collagen,” in Understanding Food (Belmont,
CA: Thomson Wadsworth, 2008), 166–167.
6S. Ando, M. Hatano, and K. Zama, “Protein Degradation and Protease
Activity of Chum Salmon (Onchorhychus keta) Muscle During Spawning
Migration,” Fish Physiology and Biochemistry 1 (1986a), 17–26; S. Ando,
F. Yamazaki, M. Hatano, and K. Zama, “Deterioration of Chum Salmon
(Onchorhychus keta) Muscle During Spawning Migration: Changes in
Protein Composition and Protease Activity of Juvenile Chum Salmon
Muscle upon Treatment with Sex Steroids, Comparative Biochemistry and
Physiology, Part B: Biochemistry and Molecular Biology 2 (1986b):
325–330.
7J. Wekell and F. Teeny, “Canned Salmon Curd Reduced by Use of Polyphos-
phates,” Journal of Food Science 53 (1988): 1009–1013.
8Sikorski, Kolakowska, and Pan, “Nutritive Composition.”
9P. O. Skjervold, A. M. B. Rora, S. O. Fjaera, A. Vegusdal, A. Vorre, and O. Einen,
“Effects of Pre‐, In‐, or Post‐Rigor Filleting of Live Chilled Atlantic Salmon,”
Aquaculture 194 (2001): 315–326.
10K. S. Morey, L. D. Satterlee, and W. D. Brown, “Protein Quality of Fish in
Modified Atmospheres as Predicted by the C‐PER Assay,” Journal of Food
Science 47 (1982): 1399–1400.
11E. Gnaiger and G. Bitterlich, “Proximate Biochemical Composition and
Caloric Content Calculated from Elemental CHN Analysis: A Stoichiometric
Concept,” Oecologia 62 (1984): 289–298.
c06.indd 103 1/14/2016 7:21:26 AM
http://www.aboutseafood.com/about/about-seafood/top-10-consumed-seafoods
104 • CULINOLOGY®
12J. Spinelli and J. A. Dassow, “Fish Proteins: Their Modification and Potential
Uses in the Food Industry,” in Chemistry and Biochemistry of Marine Food
Products, eds. R. E. Martin, G. J. Flick, C. E. Hebard, and D. R. Ward
(Westport, CT: AVI Publishing Co., 1982).
13F. Badii and N. K. Howell, “Changes in the Texture and Structure of Cod and
Haddock Fillets During Frozen Storage,” Food Hydrocolloids 16 (2002): 313–319.
14W. L. Cheuk and G. Finne, “Enzymatic Determination of Urea and Ammonia
in Refrigerated Seafood Products,” Journal of Agricultural and Food
Chemistry 32 (1984): 14–18.
15L. E. Lampila, “Seafood Lipids: Analysis and Health Benefits,” in Seafood
Quality Determination, eds. D. E. Kramer and J. Liston (Amsterdam: Elsevier
Science Publishers, 1987), 497–515.
16J. E. Osinchak, H. O. Hultin, O. T. Zajichek, S. D. Kelleher, and C.‐H. Huang,
“Effect of NaCl on Catalysis of Lipid Oxidation by the Soluble Fraction of
Fish Muscle.” Free Radical Biology and Medicine 12 (1992): 35–41.
17Z. E. Sikorski, “Protein Changes in Muscle Foods Due to Freezing and
Frozen Storage,” International Journal of Refrigeration 1 (1978): 173–180.
18H. H. Huss, “Fresh Fish: Quality and Quality Changes,” training manual
(Rome: FAO/DANIDA Training Program on Fish Technology and Quality
Control, 1998), 5–14.
19W. D. Brown, “The Concentration of Myoglobin and Hemoglobin in Tuna
Flesh,” Journal of Food Science 27 (1962): 26–28.
20Love, Chemical Biology of Fishes.
21S. Ehira and H. Uchiyama, “Determination of Fish Freshness Using the
K‐value and Comments on Some Other Biochemical Changes in Relation
to Freshness,” in Seafood Quality Determination, ed. D. E. Kramer and J.
Liston (Amsterdam: Elsevier Science Publishers, 1987), 185–207.
22D. J. Fraser, J. R. Dingle, J. A. Hines, S. C. Nowlan, and W. J. Dyer, “Nucleotide
Degradation, Monitored by Thin‐Layer Chromatography and Associated
Postmortem Changes in Relaxed Cod Muscle,” Journal of the Fisheries
Research Board of Canada 24 (1967): 1837–1843.
23J. Spinelli, “Degradation of Nucleotides in Ice‐Stored Halibut,” Journal of
Food Science 32 (1967): 38–41.
24Ehira and Uchiyama, “Determination of Fish Freshness,” 185–207.
25L. E. Lampila, V. Mohr, and D. S. Reid, “Scanning Electron Microscopic Study
of Rockfish Preserved at Either Ambient Temperature or by Isothermal
Freeze‐Fixation,” Food Microstructure 4 (1985): 11–16.
26Sikorski, “Protein Changes in Muscle Foods,” 173–180.
27National Marine Fisheries Service (NMFS), 2011, accessed August 25, 2013,
http://www.st.nmfs.noaa.gov/st1/fus/fus10/08_perita2010 .
28R. J. Price, “Fish Eggs for Caviar and Bait,” in Leaflet 21114, California Sea
Grant Marine Advisory Publication, Division of Agricultural Sciences,
University of California, 1979.
29G. S. Trout and G. R. Schmidt, “The Effect of Cooking Temperature on the
Functional Properties of Beef Proteins: The Role of Ionic Strength, pH, and
Pyrophosphate,” Meat Science 20 (1987): 129–136.
30J. C. Acton, “Effect of Heat Processing on Extractability of Salt‐Soluble
Protein, Tissue Binding Strength, and Cooking Losses in Poultry Loaves,”
Journal of Food Science 37 (1972): 244–246.
31R. Hamm and R. Grau, “The Effect of Phosphates on the Bound Water
of Meat,” Deutsch Lebensmittel Rdsch 51 (1955): 106–113.
32G. W. Schults, D. R. Russel, and E. Wierbicki, “Effect of Condensed
Phosphates on pH, Swelling, and Water‐Holding Capacity of Beef,” Journal
of Food Science 37 (1972): 860–864.
33R. G. Matlock, R. N. Terrell, J. W. Savell, et al., “Factors Affecting Properties
of Raw‐Frozen Pork Sausage Patties Made with Various NaCl/Phosphate
Combinations,” Journal of Food Science 49 (1984): 1363–1366.
34L. E. Lampila and J. P. Godber, “Food Phosphates,” in Food Additives, eds.
A. L. Branen, P. M. Davidson, S. Salminen, and J. H. Thorngate III (New
York: Marcel Dekker, 2001), 809–896.
35Food and Drug Administration, Fish and Fisheries Products Hazards and
Controls Guidance, 4th ed. (Washington, DC: Department of Health and
Human Services, 2011), 31–61.
36FDA, Fish and Fisheries Products Hazards and Controls Guidance, p. 101.
37M. A. Friedman, L. E. Fleming, M. Fernandez, P. Bienfang, K. Schrank, R.
Dickey, M.‐Y. Bottein, et al., “Ciguatera Fish Poisoning: Treatment,
Prevention and Management,” Marine Drugs 6 (2008): 456–479.
38FDA, Fish and Fisheries Products Hazards and Controls Guidance, p. 102.
39Ibid., p. 99.
40Ibid., p. 102.
41Ibid., p. 113.
42T. Noguchi and O. Arakawa, “Tetrodotoxin Distribution and Accumulation
in Aquatic Organisms, and Cases of Human Intoxication,” Marine Drugs 6
(2008): 220–242.
43FDA, Fish and Fisheries Products Hazards and Controls Guidance, p. 103.
44Ibid., pp. 91–92.
45J. Olley and S. J. Thrower, “Abalone: An Esoteric Food,” Advances in Food
Research 23 (1977): 143–186.
46FDA, Fish and Fisheries Products Hazards and Controls Guidance, p. 103.
47M. Castagna, “Shellfish: Mollusks,” in The Seafood Industry, eds. R. E. Martin
and G. J. Flick (New York: Van Nostrand Reinhold, 1990), 77–87.
48Ibid.
49F. Shahidi and J. A. Brown, “Carotenoid Pigments in Seafoods and
Aquaculture,” Critical Reviews in Food Science 38 (1998): 1–67.
50Castagna, “Shellfish: Mollusks,” 77–87.
51A. C. Hunter and B. A. Linden, “An Investigation of Oyster Spoilage,” American
Food Journal 18 (1923): 538.
52Castagna, “Shellfish: Mollusks,” 77–87.
53Z. Sikorski and I. Kolodziejska, “The Composition and Properties of Squid
Meat,” Food Chemistry 20 (1986): 213–224.
54M. P. Sieiro, S. P. Aubourg, and F. Rocha, “Seasonal Study of the Lipid
Composition in Different Tissues of the Common Octopus (Octopus
vulgaris),” European Journal of Lipid Science and Technology 108 (2006):
479–487.
55W. J. Lindberg and M. J. Marshall, Species Profiles: Life Histories and Envi-
ronmental Requirements of Coastal Fishes and Invertebrates (South
Florida)–Stone Crab. U.S. Fish and Wildlife Service. (1984). FWS/OBS‐82
/11.21. U.S. Army Corps of Engineers, TR EL‐82‐4. 17 p.
56C. Crapo, B. Paust, and J. Babbitt, “Recoveries and Yields from Pacific Fish
and Shellfish,” Marine Advisory Bulletin 37 (Fairbanks, AK: Alaska Sea Grant
College Program, 1993).
57S. Hedlund, “Langostino versus Lobster: What’s the Difference?” Seafood
Business 25 (2006): 32.
58C. P. Idyll, “The Shrimp Fishery: A Survey of Methods for Domestic
Harvesting, Preservation, and Processing of Fish Used for Food and
Industrial Products,” in Industrial Fishery Technology, ed. M. E. Stansby
(Huntington, NY: Robert E. Krieger Publishing Company, 1976), 150–170.
59Louisiana Seafood Promotion and Marketing Board, Louisiana Seafood
Products Handbook: A Guide to Species, Availability, Product Forms, and
Fisheries Facts, 3rd ed. (New Orleans: Louisiana Seafood Promotion and
Marketing Board, 1998).
60M. J. Oesterling, “Shellfish: Crustaceans,” in The Seafood Industry, eds. R. E.
Martin and G. J. Flick (New York: Van Nostrand Reinhold, 1990), 88–102.
61R. J. Miget and M. G. Haby, “Naturally Occurring Compounds Which Create
Unique Flavors in Wild‐Harvested Shrimp” (College Station, TX: Texas
Cooperative Extension and Sea Grant College Program, 2007).
62M. W. Moody, “Other Aquatic Life of Economic Significance: Alligators,”
in Marine and Freshwater Products Handbook, eds. R. E. Martin, E. P.
Carter, G. J. Flick, and L. M. Davis (Lancaster, PA: Technomic, 2000),
273–278.
63S. J. Bell, B. R. Bistrian, L. B. M. Ainsley, N. Manji, E. J. Lewis, C. Joyce, and
G. L. Blackburn, “A Chemical Score to Evaluate the Protein Quality of
Commercial Parenteral and Enteral Formulas: Emphasis on Formulas for
Patients with Liver Failure,” Journal of the American Dietetic Association 91
(1991): 586–589.
c06.indd 104 1/14/2016 7:21:26 AM
http://www.st.nmfs.noaa.gov/st1/fus/fus10/08_perita2010
CHAPTER 6 • Protein‐Based Foods: Seafood • 105
64B. Slowtzoff, “Die Ausnutzung des Fischfleisches im Vergleich mit der des
Rindfleisches und die Wirkung des Fischfleisches auf die Zusammenset-
zung des Harns,” Z. Physik. Diatet. Therap 14 (1910): 22–29.
65G. Katsch, “Normale und Veranderte Tatigkeit des Magens,” in Handbuch
der Inneren Medizin Vol. 3, Part 1, eds. G. von Bergmann and R. Staehelin,
(Berlin: Springer, 1926).
66K. H. Lee and H. S. Ryu, “Evaluation of Seafood Protein Quality as Predicted
by C‐PER Assays,” in Seafood Quality Determination, eds. D. E. Kramer and
J. Liston (Amsterdam: Elsevier Science Publishers, 1987), 473–485.
67J. C. Acton and C. L. Rudd, “Protein Quality Methods for Seafoods,” in
Seafood Quality Determination, eds. D. E. Kramer and J. Liston (Amsterdam:
Elsevier Science Publishers, 1987), 453–472.
68E. Geiger, and G. Borgstrom, “Fish Protein: Nutritive Aspects,” in Fish as
Food Vol. 2, ed. G. Borgstrom, (New York: Academic Press, 1962).
69H. A. Bremner, Post Mortem Breakdown of the Myotendinosus Junction in
Fish, doctoral dissertation, University of Tasmania, 1993.
70A. D. Woyewoda, S. J. Shaw, P. J. Ke, and B. G. Burns, “Recommended
Laboratory Methods for Assessment of Fish Quality,” Canadian Technical
Report of Fisheries and Aquatic Sciences No. 1448 (Canada Department of
Fisheries and Oceans, 1986).
71N. V. Ralston, C. R. Ralston, J. L. Blackwell, and L. J. Raymond, “Dietary and
Tissue Selenium in Relation to Methylmercury Toxicity,” Neurotoxicology 29
(2008): 802–811.
72National Research Council, Toxicological Effects of Methylmercury
(Washington, DC: National Academy Press, 2000).
c06.indd 105 1/14/2016 7:21:26 AM
7
106 • CULINOLOGY®
Protein‐Based Foods:
Vegetable Sources of
Protein and Protein
Complementation
Introduction:
The Culture of
Vegetarian Diets
Animals are defined by where they get their protein. The
terms herbivore , carnivore , and omnivore are used to describe
animals whose diets are exclusively vegetarian, meat‐eating,
or a combination of the two. The success of the human spe-
cies is largely because they are omnivores (eaters of every-
thing). Early humans were opportunistic eaters, able to derive
nutrition from virtually any food source, making survival
much more likely. Today, in developed countries, where star-
vation is less of a threat, choice is the number‐one factor in
what humans eat. While we are still an omnivorous species
by nature, humans can survive quite well on an exclusively
vegetarian diet, if managed properly.
In the United States, consumption of large quantities of
meat protein is relatively common, while most other cultures
consume much smaller portions of meat (or none at all). As
Americans become more health conscious and more aware
of the widely reported links between diet and disease, how-
ever, various degrees of vegetarian diet have become more
common.
There are many types of vegetarian diet. Vegans are so‐
called strict vegetarians who choose to eat no animal prod-
ucts, not even eggs or milk‐derived products such as cheese,
yogurt, or ice cream, and some even exclude honey as the
product of an exploited species. Another common type of
vegetarian diet is lacto‐ovo or ovo‐lacto , which allows eggs
and dairy in the diet in addition to plant‐based foods.
Pescatarians eat seafood. Flexitarians practice varying
degrees of vegetarianism but do occasionally eat animal‐
derived foods.
The results of a Harris poll conducted in 2008 (on behalf of
the Vegetarian Times ) indicate as many as 1 million practicing
vegans in the United States, nearly 7½ million vegetarians,
and nearly 23 million who follow a “vegetarian‐inclined” diet.
And the market for vegetarian foods is growing. According to
a study done by Mintel, the value of vegetarian food sales at
retail only (no foodservice figures are included) was $815 mil-
lion in 2001 and $1.4 billion in 2008; it is expected to grow to
$1.6 billion in 2015.
The rationale of people who follow a vegetarian diet may
be moral, ethical, religious, and/or political as well as health‐
based. Vegetarians are often concerned with animal welfare,
the environmental impact of the diet (such as recyclable
packaging and local, sustainable farming), and its socioeco-
nomic impact (such as the Fair Trade movement).
When formulating vegetarian foods, it is important to
create products that appeal to this spectrum of beliefs and
values as well as provide an adequate source of vegetable
protein—and of course, something that tastes good. It is also
important to be aware that many of the functional ingredi-
ents in the product developer ’ s toolbox may themselves be
animal‐derived. It is easy for the vegetarian consumer to
identify the difference between a hamburger and a veggie
burger but much more difficult to know whether any of the
multitude of ingredients in a snack seasoning have an animal
source. For example, in 2002 McDonald ’ s was forced to pay
$10 million to settle a lawsuit brought on behalf of members
of the American Hindu vegetarian community who ate
McDonald ’ s French fries because the giant restaurant chain
claimed they were cooked in pure vegetable oil. Unfortunately,
the vegetarians were unaware that this oil contained a natu-
ral flavor that was partially derived from beef fat.
Other hidden animal‐derived ingredients include gelatin
(used to make marshmallows and gummy candies and as a
thickener in some yogurts), lard (traditional in pie crusts),
milk products (in many candies and chocolate), casein (ironi-
cally, used in the manufacture of margarine and creamy salad
dressings), whey protein (used to bulk up the protein in boxed
Lead Author: Natalie Pitchford Levy, CCS®, Quality Control Manager , Bedemco, Inc.
Contributor: Hinnerk von Bargen , CHE, Associate Professor in Culinary Arts , Culinary Institute
of America
c07.indd 106 1/22/2016 1:05:34 PM
CHAPTER 7 • Protein‐Based Foods: Vegetable Sources of Protein and Protein Complementation • 107
cereals and provide structure in cake mixes), rennet (an
enzyme derived from calf stomachs used in the manufacture
of cheese), and bone char (used in the refining of sugar to
remove color impurities in the syrup prior to crystallization
into table sugar).
It is generally recognized that a vegetarian diet is not only
healthy but may be even healthier than the typical meat‐
centric diet followed by most Americans. The 2010 version of
Dietary Guidelines for Americans, a report issued by the U.S.
Department of Agriculture (USDA) and the U.S. De partment
of Health and Human Services (DHHS) every five years,
states:
In prospective studies of adults, compared to non‐vegetar-
ian eating patterns, vegetarian‐style eating patterns have
been associated with improved health outcomes—lower
levels of obesity, a reduced risk of cardiovascular disease,
and lower total mortality. Several clinical trials have
documented that vegetarian eating patterns lower blood
pressure.
On average, vegetarians consume a lower proportion of
calories from fat (particularly saturated fatty acids), fewer
overall calories, and more fiber, potassium, and vitamin C
than do non‐vegetarians. Vegetarians generally have a lower
body mass index. These characteristics and other lifestyle
factors associated with a vegetarian diet may contribute to
the positive health outcomes that have been identified
among vegetarians.
Nutritionally speaking, vegetarian diets tend to be lack-
ing in those nutrients found mainly (or even exclusively) in
non‐vegetarian diets. These include vitamin B
12
(found only
in foods derived from animals) and the essential omega‐3
fatty acids DHA and EPA (primarily from fish). These nutri-
ents must be taken as supplements if individuals are unwill-
ing to consume meat from any source (aquatic or terrestrial).
Other essential vitamins and minerals can be found in cer-
tain vegetables: calcium in collards, bok choy, kale, and tur-
nip greens; iron in legumes, raisins, nuts and seeds, and in
fortified foods such as breakfast cereals; vitamin D in forti-
fied soymilk. Lysine, threonine, methionine, and tryptophan
are the essential amino acids typically lacking in the vege-
tarian diet. As such, they are known as the limiting amino
acids, and are found in the shortest supply in incomplete
proteins (those found in most plant sources). They can,
however, be successfully provided via the practice of protein
complementation.
Protein complementation occurs when two vegetable
proteins (legumes and grains, for example), each containing
the amino acid that the other lacks, are combined so the
body gets both. In this way, all of the limiting amino acids can
be acquired, as long as the diet contains enough of the vege-
table varieties that provide the lacking amino acids in suffi-
cient concentrations to have a significant effect in the body.
For instance, legumes are low in methionine and high in
lysine; grains are low in lysine and high in methionine. So if
rice (grain) and beans (legume) are eaten together, both of
the limiting amino acids are consumed. Contrary to earlier
belief, protein complementation need not be done at the
same meal. As long as the two foods are consumed within a
few hours of each other (or even the same day), the body
treats them as a complete protein.
Vegetable Sources
of Protein
Legumes
Legumes are among the simplest and oldest sources of vege-
table protein, and their cultivation and culinary use is well
developed. They are fruits or seeds of plants from the legume
family, with characteristic pods that split along the center
into two sides with a single row of seeds. Legume plant roots
contain nodules with nitrogen‐fixing bacteria that capture
nitrogen in the soil, improving soil fertility. In addition, the
seeds (peas and beans) contain significant amounts of pro-
tein compared to other plants.
Types of Legumes
Legumes include fresh beans and peas with edible pods that
are picked while immature, such as green beans, wax beans,
haricots verts, snow peas, and sugar snap peas. The category
also includes fresh shelling beans and peas whose pod is
removed to reveal an edible seed—for example, sweet peas,
fava beans, edamame (soybeans), flageolets, and lima beans.
Lentils and peanuts are also in the legume family.
Large legume varieties are allowed to mature and dry on
the vine and then are harvested, shelled, and further dried
before storage and sale. Dried legumes that are not raised for
fresh use or for oil extraction are known as “pulses.” Dried
beans include black, kidney, pinto, Great Northern, cannel-
lini, garbanzo (chickpeas), black‐eyed peas, lentils, and split
peas (green and yellow). When selecting dried beans, look for
uniform color, few chipped or broken beans, and an absence
of shriveled skins.
Although they are technically a legume, peanuts are dis-
cussed in the section on nuts and seeds, as they are most
commonly sold and processed like nut products.
Fresh, frozen, and dried beans do not typically contain
additives or processing aids. Canned beans typically contain
beans, water, and salt (or kombu seaweed, in more natural
versions), and may contain firming agents such as calcium
chloride or color preservatives such as disodium EDTA.
Storage and Shelf Life
The varying moisture content of different forms of beans
contributes to differences in handling and shelf life. Fresh
beans are chosen for bright color and firm texture, with no
brown or soft areas. Fresh beans (such as raw green beans)
contain 80 to 90 percent moisture and should be washed just
prior to use, as additional water can compromise shelf life in
storage. If properly stored in a sealed container under refrig-
eration, fresh beans can last several days.
Uncooked dried beans typically have moisture below 14
percent and are best if used within one year of drying. Beyond
c07.indd 107 1/14/2016 7:21:58 AM
108 • CULINOLOGY®
this time, color may begin to fade and they may get even drier,
causing cracks, dull flavor, and longer cook times. Dried beans
are sold and may be stored in polyethylene bags; however, for
long‐term storage, bulk dried beans should be stored in
airtight containers in a cool, dry location to prevent infesta-
tion. Rehydration through cooking in boiling water typically
increases moisture content to between 50 and 70 percent.
This greatly reduces shelf life, and cooked beans may begin to
exhibit off odors indicative of microbial growth after several
days. However, commercially canned beans are cooked and
effectively sterilized in the can through a retort process; they
can last over two years in their original sealed can.
A food processor must be aware of the shelf‐life limita-
tions of fresh beans and non‐retort cooked dried beans when
using them in fresh or refrigerated products. Cooked beans
are rich in protein and vitamins and minerals and provide an
ample growth medium for bacteria, yeasts, and molds.
Improper canning can lead to growth of anaerobic bacteria
such as Clostridium botulinum, so strict HACCP (Hazard
Analysis Critical Control Points) guidelines with time, tem-
perature, and pressure controls must be followed when
canning beans for storage, particularly because they are a
low‐acid (above pH 4.6) food.
Cooked dried beans may be frozen to extend shelf life for
several months. Frozen cooked dried beans should be thawed
slowly to maintain their shape and reduce splitting. For com-
mercial usage (soups, chili, etc.), thaw cooked beans under
refrigeration overnight prior to usage, or they may be added
frozen to the kettle toward the end of heating as long as the
cook time and temperature of the product in the kettle is
adjusted to compensate for the sudden drop in temperature,
and to provide the required kill step. Some mild grittiness in
texture may occur when freezing cooked beans; however,
bean‐based soups and stews freeze quite well because the
liquid medium and other ingredients can mask off‐textures.
Preparation and Cooking Methods
Fresh beans with edible pods are best cooked quickly in large
volumes of salted water to avoid loss of color and flavor.
Commercially sourced beans typically have the stem‐end and
strings along the spine of the pods removed by the supplier.
The beans can be steamed, boiled, sautéed, or added to a
composed dish at the end of cooking to maintain crisp‐
tender texture and vibrant color.
The chlorophyll in freshly cooked green‐colored beans
reacts with acidic foods to turn from bright green to a dull
olive color. It can also be degraded by overcooking. This also
occurs at a very slow rate in frozen raw beans during long
storage. The only way to prevent this color change is to cook
green vegetables in large volumes of water, uncovered (to
allow vaporized acids from the beans to escape), and to shock
or chill them rapidly as soon as they reach the appropriate
doneness. The addition of an alkalizing agent (such as sodium
bicarbonate) to cooking liquid can inhibit, but not eliminate,
this dulling of chlorophyll in the cooking of fresh beans. That
said, this is not recommended since bicarbonate can also
destroy the water‐soluble vitamins (especially vitamin C) and
can degrade the structural components in vegetables, leading
to limp, mushy texture.
Blanching can act as a par‐cooking step for fresh beans
in frozen meals and must be taken into account when
determining the overall cook time of a prepared bean‐
containing meal. The different components of a mixed dish
may need to be combined at various levels of doneness to
ensure a brief reheating by the consumer properly heats the
entire product without overcooking or undercooking indi-
vidual ingredients.
It is important to note that unlike the green color in raw
vegetables, which is unaffected by exposure to fluorescent
light, the green color in refrigerated blanched vegetables is
sensitive to extended fluorescent light exposure (as typical of
a refrigerated supermarket shelf, where the light is always
on); it starts to turn khaki‐colored in only a few hours.
Therefore, it is critical for the product developer to know
where and how a finished product containing blanched green
vegetables will be merchandized in order to determine not
just the best processing methods but also the best packaging.
In the above example, the best solution might be to place the
vegetables in an opaque package to block exposure to light.
FIGURE 7.1 Protein in beans (cooked).
Bean (1 cup) Protein (grams)
Adzuki (Aduki) 17
Anasazi 15
Black beans 15
Black‐eyed peas 14
Cannellini (white beans) 17
Cranberry beans 17
Fava beans 13
Garbanzos (chickpeas) 15
Great Northern beans 15
Green peas, whole 9
Kidney beans 15
Lentils 18
Lima beans 15
Mung beans 14
Navy beans 16
Pink beans 15
Pinto beans 14
Soybeans 29
Split peas 16
Source: USDA National Nutrient Database for Standard Reference,
http://www.nal.usda.gov/fnic/foodcomp/search.
c07.indd 108 1/14/2016 7:21:58 AM
http://www.nal.usda.gov/fnic/foodcomp/search
CHAPTER 7 • Protein‐Based Foods: Vegetable Sources of Protein and Protein Complementation • 109
Dried beans require intensive measures to rehydrate
them. The protein and starch are stored in the cotyledons
(two inner halves) of the legume, covered by an outer seed
coat made of hemicellulose structures. A small pore called
the hilum (which previously attached the bean to the plant)
is the only break in the outer seed coat that allows water to
penetrate into the bean. As the water infuses the bean
through the hilum, the starches inside absorb water, caus-
ing the bean to expand. The expansion breaks down the
cell walls, softens the hemicellulose, and dissolves the pec-
tin. The bean can then swell proportionately. As the
starches and proteins inside the bean cook, the flavors are
developed.
Dried beans should be soaked prior to cooking to speed
the cooking process and to remove indigestible compounds.
In addition, dried beans often contain agricultural contami-
nants and should be rinsed with water and checked for
stones. Beans may be presoaked using either the overnight
cold‐soak or the hot quick‐soak method. To quick soak, bring
rinsed beans to boil in surplus water to cover, then simmer
for 2 minutes, remove from heat, cover, and allow to soak for
1 hour. The quick‐soak method typically results in more split
beans than the gentler overnight method.
After either presoak method, the soak water is discarded
and the beans are cooked in fresh water that is brought to a
boil and then simmered for the specified time (typically from
15 minutes to 1 hour, depending on size and variety). Lentils
and split peas are small and delicate enough to not require
presoaking; they may be cooked just after rinsing.
It is important to note how other cooking ingredients can
affect the cook time and characteristics of beans. Several fac-
tors can slow the cooking of beans:
• Minerals (such as calcium or magnesium) in hard water
strengthen cell wall pectin by cross‐linking.
• Acids (lemon juice, tomato juice, wine) stabilize hemi-
cellulose walls.
• Molasses and sugar strengthen cell walls, cross‐ link pectin,
stabilize hemicellulose, and slow starch granule swelling.
Any of these cause slower cooking and the potential for
toughening of the outer skin if added early in the normal
cooking process. However, they are useful in slow‐cooked
applications such as chili or baked beans because they pro-
tect the bean from cooking too quickly, and inhibit splitting.
Molasses is particularly useful, as its acidity, sugar, and min-
eral content all contribute to longer cooking times.
Adding salt to bean cooking water sparks debate among
chefs and food scientists. If added early, salt begins to slow
the rate of water absorption; however, after a time it adds fla-
vor to the beans and speeds the cooking process. The U.S.
Dry Bean council recommends adding 0.5 percent salt to
bean cooking liquid.
One pound of dried beans yields about 6 cups cooked,
depending on the variety. Rehydration typically causes a
doubling in volume, so ½ cup dry equals approximately 1 cup
cooked.
For some preparations, cooked legumes are pureed (as for
refried beans and hummus). Their high starch content also
makes legumes perfect candidates for dry heat preparations
with a crunchy, crisp texture profile. Middle Eastern cuisine
is famous for falafel, which are deep‐fried fritters made from
soaked chickpeas coarsely ground with spices, herbs, and
aromatics.
Nutritional Profile
Dried beans and other pulses are a good source of protein (17
to 25 percent), with significantly less fat than meat. Beans
(other than soybeans) are not a complete protein, as they are
limiting in the essential amino acid methionine. They are,
however, rich in other essential amino acids, such as lysine
and cysteine, which are limiting in other non‐meat proteins
such as grains and corn. Combining beans with whole grains
(protein complementation) such as wheat, rice, or corn (typi-
cally 2 parts grain to 1 part bean) can complete the amino
acid profile. The nutritional value of dried beans, peas, lentils
and chickpeas is so great, especially in parts of the world
where hunger and starvation is common, that the United
Nations has declared 2016 the “International Year of Pulses”
to heighten public awareness of the nutritional benefits of
pulses as part of sustainable food production aimed toward
food security and nutrition. In addition, pulses, like other
leguminous plants, have nitrogen‐fixing properties that can
contribute to increasing soil fertility and have a positive
impact on the environment.
Beans are slow to metabolize and can provide a carbohy-
drate source that does not dramatically increase blood sugar
levels, and they contribute potassium, zinc, iron, calcium, and
several B vitamins to the diet.1 It is important to soak beans
not only to speed cook time but also to soften them and
remove phytic acid (an antinutritional compound that binds
to minerals, inhibiting their absorption in the body) and to
assist in leaching out some of the indigestible and flatulence‐
causing carbohydrates (oligosaccharides) stachyose and raffi-
nose. Humans do not possess the enzyme required to break
down these compounds to simple sugars, but bacteria in the
lower intestine are able to digest them, producing copious
amounts of methane, carbon dioxide, and hydrogen as a result.
Interestingly, these oligosaccharides (now known as prebiot-
ics) provide a food source for these beneficial gut bacteria
(known as probiotics, or live microorganisms that confer a
health benefit to the host). The soaking does not eliminate the
oligosaccharides but does reduce the level. Some cuisines add
the herbs epazote and summer savory to cooking beans for
the same purpose.
Traditional cultures have long appreciated the value of
beans in the diet and intuitively combined beans with their
complementary grains in both farming and cuisine. Beans,
corn, and squash crops were grown as the “three sisters” in
Native American agriculture, and traditional complementary
pairings such as rice and beans, barley and lentils, and corn-
bread and black‐eyed peas sustained humanity for many
years.
c07.indd 109 1/14/2016 7:21:59 AM
110 • CULINOLOGY®
Dried beans may also be sprouted or soaked in water until
they begin to germinate. Sprouting is accompanied by the
enzymatic hydrolysis of protein; large proteins are broken
down into amino acids. Likewise, large carbohydrates are
broken down into smaller, more easily digestible sugars.
Thiamin increases fivefold, and niacin content doubles.
Carotene and vitamins C and E increase. In fact, the vitamin
C content of many sprouts is as rich as that of tomatoes. Overall
digestibility is vastly improved. Some common sprouting
beans include mung and adzuki. Sprouting can take from
three to five days, during which the bean opens up and releases
a small shoot that can be eaten raw ; however, it is best to
lightly cook all sprouted beans if large quantities will be
eaten. Although a favorite of raw foodists, sprouts are notori-
ous for harboring bacteria such as salmonella, so sprouting
must be undertaken with care and HACCP principles in mind
(such as attention to water quality, container, frequency of
changing sprouting water) to prevent foodborne illness.
Soybeans and Soy Foods
The soybean (Glycine max) is a legume that can produce at
least twice as much protein per acre as any other major
vegetable or grain crop, 5 to 10 times more than land set aside
for dairy cattle, and up to 15 times more protein per acre than
meat production.
Soy‐based foods have increased in visibility and consump-
tion in the U.S. market over the past several years, partly due
to greater acceptance of vegetarian diets and partly because of
health claims of soy as a miracle food. Soybeans and soy prod-
ucts remain controversial and may pose health risks as well as
benefits (especially if consumed in large quantities). However,
in October 1999 the U.S. Food and Drug Administration (FDA)
authorized the following claims for soy protein:
1. 25 grams of soy protein a day, as part of a diet low in
saturated fat and cholesterol, may reduce the risk of heart
disease. A serving of [name of food] supplies __ grams of
soy protein.
2. Diets low in saturated fat and cholesterol that include 25
grams of soy protein a day may reduce the risk of heart
disease. One serving of [name of food] provides __ grams
of soy protein.
It should also be noted that the FDA declared soy as one of
the “major eight” allergens in the Food Allergen Labeling and
Consumer Protection Act (FALCPA) of 2004. (In fact, it is the
second most allergenic food, after peanuts.) Therefore, any
product that contains soybeans or soy derivatives must be
clearly labeled with soy or soybeans in the ingredient state-
ment and/or in a comprehensive statement after the ingredi-
ent list that includes any of the major eight allergens in the
food (for example, “Contains soy”).
Fresh soybeans (edamame) can be eaten as a whole‐food
source of protein. Mature dried soybeans can be hydrated
and then fermented into tempeh, miso, or soy sauce, further
processed into soy milk and then tofu, or manufactured into
soy protein isolates and concentrates and textured vegetable
protein (TVP). Soy isolates and TVP can then be used to cre-
ate a new generation of processed soy food analogs such as
soy cheese, soy sausage, and other meat replacements.
The nutritional value of soy, as well as its texture, flavor,
culinary use, and food manufacturing applications, vary
widely as the bean is processed into these forms.
General Nutrition of Soy
Although soy is typically limiting in the sulfur‐containing
amino acids methionine and cysteine, it is commonly touted
as the only complete plant protein equivalent to animal pro-
tein, as all nine essential amino acids are present (and acces-
sible) in comparable ratios to those needed for human growth
and health. The Protein Digestibility Corrected Amino Acids
Score (PDCAAS) is used by the USDA to determine protein
quality. According to the PDCAAS system, soybeans have a
score of 0.91, while soy protein isolate has the maximum
score of 1.0 (the same as egg whites), followed by milk and
meat proteins.2
However, studies have shown that more soy protein might
be needed than egg‐white protein to maintain healthy nitro-
gen balance in the body. Consuming soy protein sources
along with protein foods high in methionine (whole wheat,
rice, corn, eggs) can supplement this amino acid to create a
more complete protein source, according to the USDA National
Nutrient Database for Standard Reference.
Soybeans are rich in isoflavones (a class of phytoestro-
gens, or plant hormones, chemically similar to estrogen).3
These may contribute to the functionality of soy in alleviating
some of the symptoms of menopause (North American
Menopause Society, 2000) and in preventing certain cancers
and osteoporosis (Messina, 2003). However, they are also
controversial and contribute to cautionary consumption of
soy foods, especially among males.4 That said, a recent article
summarizing the findings of dozens of clinical trials and epi-
demiological reviews found that neither soy protein nor iso-
flavones had any effect on reproductive hormones in men.5
Due to the apparent significant health functionality of soy
(for better or for worse), it seems wise to consume soy prod-
ucts in moderation as part of a varied and healthy diet.
Soy Milk
Soy milk is the liquid extracted from ground, hydrated, and
cooked soybeans. Plain soy milk is bright white to tan; its fla-
vor varies depending on the type of soy milk and processing
but can include beany, grassy, milky, nutty, metallic, earthy,
and doughy notes.
Soybeans or soy flour are soaked in water for a minimum
of several hours or overnight, ground with added water to
create a paste, and then boiled. Then the liquid is filtered to
remove the insoluble soy pulp; therefore, soy milk is not tech-
nically a whole‐food form of soy.
The boiling step is important in inactivating protease
(trypsin) inhibitors, naturally found in raw soybeans, which
prevent the pancreas from generating the necessary enzymes
to digest proteins.6 Heating also inactivates lipoxygenase
c07.indd 110 1/14/2016 7:21:59 AM
CHAPTER 7 • Protein‐Based Foods: Vegetable Sources of Protein and Protein Complementation • 111
present in the beans, which can create free radicals, rancidity,
and off flavors. Most varieties of soy milk sold in the United
States are certified organic and made from non‐GMO (genet-
ically modified organism) soybeans, both factors of impor-
tance to the majority of soy milk purchasers.
Traditionally, soy milk was available mainly in shelf-stable,
ultra‐high temperature (UHT) aseptic packages, typically
laminated “juice‐box”‐style cartons. These shelf‐stable con-
tainers are sterile and hermetically sealed, like cans, and can
be stored unopened without refrigeration. Once opened,
however, they must be kept refrigerated. Brands such as
Edensoy and Vitasoy are examples.
Refrigerated soy milks are also available, competing
directly with cow’s milk and claiming cow’s milk‐like flavor
and texture. Brands such as Silk (White Wave), 8th Continent,
Organic Valley, and even private‐label store brands are widely
available.
Refrigerated soy milks have similar formulations to shelf‐
stable soy milks; however, they are not packaged in sterile
aseptic boxes but rather traditional gable‐top milk contain-
ers. Refrigerated soy milk was created to have the appear-
ance, flavor, and mouthfeel that appeals to the mainstream
milk consumer. It is similarly pasteurized (typically UHT) and
sometimes contains carrageenan (seaweed extract), which
contributes to mouthfeel. It is also commonly available in
sweetened and unsweetened versions, as well vanilla and
chocolate flavored. Refrigerated soy milk manufacturers also
produce soy creamers, soy yogurt, soy ice cream, dips, and
spreads.
Ingredients
Traditional, organic, minimally processed commercial soy
milk typically contains some variation of the following ingre-
dients: purified or filtered water, organic soybeans, sweetener
(dried organic cane juice and/or malted organic wheat extract
and/or organic barley extract), calcium carbonate (for dietary
calcium), kombu seaweed (for minerals [optional]), salt or sea
salt, and carrageenan (for texture and mouthfeel [optional]).
Soy milk may also contain natural flavors (such as cocoa
powder or vanilla) and may also be enriched, like cow’s milk,
with zinc oxide, riboflavin (vitamin B2), vitamin A acetate (or
palmitate), vitamin D2, and vitamin B12. Soy milks are also
being fortified with fiber, extra calcium, and omega‐3 oils.7
Fortification of soy milk makes it an excellent source of nutri-
ents, such as vitamin B12 and omega‐3 oils, that are difficult to
attain in a strict vegetarian diet.
New, more highly processed fat‐free and light versions of
soy milks can be found with added fillers, emulsifiers, flavor-
ings, and sweeteners. The ingredient lists of these soy milks
may include soybean oil (for mouthfeel), calcium phosphate
(for dietary calcium and pH), potassium citrate (for dietary
potassium and pH), magnesium phosphate (for dietary mag-
nesium), sodium phosphate (to stabilize flavor), dipotas-
sium phosphate (for dietary potassium), soy lecithin (to
emulsify), xanthan gum (for texture and to emulsify), natural
and artificial flavors, and sucralose (to sweeten without
added sugar).8
Storage and Shelf Life
Shelf‐stable soy milk typically can be stored unopened at
room temperature for one year from production. Once the
package is opened, shelf life is reduced to 5 to 10 days with
refrigeration. Spoiled soy milk tends to exhibit a sour smell,
sour beany flavor, and watery, clumped texture.
The soy protein in shelf‐stable soy milk without stabilizers
may coagulate into small lumps, which usually break apart
upon shaking. These are safe to consume if not accompanied
by signs of spoilage, such as a sour smell or taste.
Unopened refrigerated soy milk keeps fresh under refrig-
eration until the best‐by date stamped on the carton. Once
opened, it lasts 7 to 10 days if refrigerated.
Although both forms of soy milk can be frozen, freezing is
not recommended. Frozen and thawed soy milk is safe to
consume; however, texture deteriorates markedly. The soy
protein and soybean oil separate from the liquid on thawing,
causing unattractive flocculation that cannot be reintegrated
through blending.
Preparation and Cooking Methods
For manufacturers of vegetarian prepared foods, it is essential
to know how to use soy products as ingredients. Both shelf‐
stable and refrigerated soy milks can be substituted 1:1 for
dairy milk in recipes. However, soy milk (especially shelf stable)
should not be brought to a rolling boil when used in gravies or
sauces, as it may curdle as the stabilizers lose effectiveness. It
should be heated to a simmer only, then reduced in heat.9
Soy milk cannot typically be whipped into a stable cream
due to low fat content.10 However, oil and stabilizers may be
added to create soy whipped cream.
Soy milk can be coagulated to make tofu, soy cheese, or
custards such as flan and crème caramel.
Nutritional Profile
Calcium naturally occurs in soybeans; however, in order to
compete with cow’s milk, soy milks must be fortified with
additional calcium, some claiming up to 20 percent of the
daily value per 8‐ounce (89 mL) serving, depending on the
level of fortification.11 Soy milk can also provide naturally
occurring omega‐3 essential fatty acids. Soy milk is a vegeta-
ble product and contains no cholesterol, and it is very low in
saturated fat yet high in protein.12
Tofu
Tofu is a soft, cheese‐like soy product made from coagulated
and pressed soy milk. Known also as bean curd, it is a tradi-
tional part of Asian cuisine.13
To make tofu, soybeans are prepared as for soy milk. As in
cheese‐making, coagulants are dissolved in water and then
stirred into the boiled soy milk until it curdles into a gel or
curd.14 The curd is separated from the soy whey, pressed into
blocks, and packaged.
Typical coagulants include acids and various mineral salts,
such as calcium sulfate, seawater, nigari (magnesium chlo-
ride), and glucono delta lactone (GDL).15 The coagulants
c07.indd 111 1/14/2016 7:21:59 AM
112 • CULINOLOGY®
used determine the final texture of the tofu, as they create
curds with different‐sized pores. Tofu products can be divided
into fresh tofu, which is produced directly from soy milk, and
processed tofu, made from fresh tofu.
Fresh Tofu
Fresh tofu is usually sold in water to maintain its moisture
content. Pasteurized versions sold in the U.S. market can be
eaten raw. The excess liquid should be drained before
cooking.
Fresh tofu is typically white to light beige in color. It is clas-
sified by the density of the curd, which varies depending on
the amount of water removed from it. Varieties include
silken/soft, firm, extra firm, and dried. All fresh tofu has a raw,
beany, grassy, green flavor. Fresh tofu is typically sold as
organic and non‐GMO in U.S. supermarkets. Softer styles
work well in soups, sauces, desserts, and shakes. Generally,
firmer tofu can act as meat replacements in many recipes.
Silken or Soft
Silken tofu has the highest moisture content, as the soy
milk is curdled directly in the package and the product is
un drained.16 It has the texture of flan or fine custard. Silken
tofu can simulate the thick, custardy mouthfeel of dairy
ingredients for vegan menu items such as cream‐style dips
and spreads. Vegan versions of sour cream, mayonnaise dips,
cream soups, lasagna filling, milk shakes/smoothies, cheese-
cake, pudding, and custards can be prepared using silken tofu.
Soft tofu is very similar to silken, with a slightly thicker tex-
ture. It can be used in similar ways and works well gently sliced
or crumbled into soups, salads, and sauces. Soft tofu can also
be mashed and mixed with chopped vegetables or meat before
being cooked. The mixture can be bound with starch and deep
fried like a dumpling, or steamed to make a meat loaf or meat-
balls. It is also commonly used as a filling in dumplings.
Silken and soft tofu cannot typically withstand harsh
cooking methods such as deep frying and stir‐frying. These
products work well in blending and whipping and in gentler
cooking such as steaming, baking, or simmering in soup
broth.
Firm and Extra‐Firm
In firm and extra‐firm tofu, the soy curd is cut, the liquid is
strained through muslin or cheesecloth, and the curd is
pressed into a cake. One can often see the fine‐grained pat-
tern of the straining cloth on the surface of the tofu. Firm and
extra‐firm tofu can be used in recipes that require more vigor-
ous cooking methods.
Firm tofu is still very moist, with a more resilient, bouncy
outer skin and inner custardy texture. Firm tofu can be cut into
distinct shapes such as slices or diced bits, which can be stir‐
fried, pan‐fried, or deep fried. These cooking methods yield a
crisp exterior and soft interior. Firm tofu can also be crumbled
or cut into small cubes to replace eggs in vegan egg salads.
Extra‐firm tofu is created with a coagulant that creates a
drier, more brittle texture and stronger cake. It has a firmer
texture throughout and is easily cut into shapes that can hold
up to the most intense cooking methods such as pan‐frying,
stir‐frying, deep frying, grilling, broiling, and even simmering
in stews and casseroles.
As firm and extra‐firm tofu are generally served in intact
pieces, it is beneficial to remove as much of the water from the
product as possible, followed by marination to infuse flavor
into the interior. Marinating works best if the tofu is cut into
pieces or gently pricked with a fork along the surface. Tofu can
be pressed for several hours between two plates or flat pans,
with the top pan weighted down, to further firm the texture
and reduce residual water that might splatter when frying.
Draining excess liquid, slicing, and freezing firm and extra‐
firm tofu increases pore size, creates a yellower color, and
develops a tougher, chewier texture. The water in the tofu
expands as it freezes, creating larger openings to absorb liquid
once thawed. This allows the tofu to absorb additional mari-
nade. The chewy texture of frozen and thawed tofu makes it a
suitable meat substitute when grilling and barbecuing.
Dried Tofu
Dried tofu, made from extra‐firm tofu, has a rubbery texture
that can be crumbled, sliced, or pressed and cut or shredded
into long, noodle‐type strings. Dried tofu is usually not eaten
raw but stewed in a mixture of soy sauce and spices. The noo-
dle form can be served cold or stir‐fried.17
Processed Tofu
Fried Tofu
Firmer forms of tofu are sometimes sold cut into bite‐size
pieces and fried in oil to create a golden crisp exterior and
airy interior. Tofu may be lightly dredged in cornstarch before
frying to absorb excess surface moisture and impart a crisp
texture. These pieces may be eaten alone, with a dipping
sauce, or added to recipes such as hotpots and stews.
Frozen Tofu
In Asia (and some Asian markets), thawed and sliced frozen
tofu called thousand‐layer tofu can be found. Made from fro-
zen soft tofu, the ice crystals create large layered cavities, a
tougher texture, and a yellowish color. Thousand‐layer tofu
may be squeezed of excess moisture and eaten as is with a dip
or dressing, or it can be grilled, fried, or roasted and used as a
meat replacer in a variety of Asian‐inspired vegetarian
entrées.
Freeze‐Dried Tofu
Tofu is also available in freeze‐dried blocks and cubes from
Asian food suppliers. These must be soaked in water before
eating. This product is commonly simmered in broth, rice
wine, and/or soy sauce. It has a spongy texture and absorbs
the soaking liquid flavor. This type of tofu is also added in
smaller pieces, along with other freeze‐dried toppings, to
instant soups such as miso soup.
Flavored Tofu
Sweeteners and flavorings can be mixed into soy milk before
curdling. Fresh tofu marinated in sauces and spices is also
becoming more common in Western markets.
c07.indd 112 1/14/2016 7:21:59 AM
CHAPTER 7 • Protein‐Based Foods: Vegetable Sources of Protein and Protein Complementation • 113
Sweet: Most sweet tofu is silken and served cold. Common
flavors include peanut, almond, mango, and coconut.
Savory: Varieties include egg tofu (with added eggs), chili‐
peppered egg tofu, and tofu marinated with garlic, spices, or
soy sauce.
Fermented Tofu and Tofu By‐Products
Asian suppliers also offer versions of fermented tofu such as
pickled or “stinky” tofu, as well as by‐products such as tofu
skin and okara.
Pickled or preserved tofu are cubes of air‐dried tofu fer-
mented under hay and then soaked in various flavored acidic
liquids.18 Due to its strong flavor, pickled tofu is often used in
small amounts as a condiment or to flavor stir‐fries or braised
dishes.
Stinky tofu is a soft tofu fermented in vegetable and fish
brine.19 The blocks have a soft texture and a strong, pungent,
almost cheese‐like aroma, rotten to some but appreciated by
aficionados. When fried, it develops a crisp rind that can be
topped with Asian sauces.
Tofu skin, or soy milk skin, is produced by boiling down
soy milk in a shallow pan to produce a soy protein–lipid film
that is collected and dried into yellowish sheets. It has a
pliable, rubbery texture. The skin can be used as a wonton
wrapper, folded or shaped to mimic meat, or formed into
sticks and dried into tofu bamboo.
Okara is the soy pulp (press cake) remaining after soy milk
is filtered. It is quite flavorless and has a sawdust‐like mouth-
feel, although it maintains some nutrient value, primarily as
dietary fiber. Okara may be used to make porridge, stew, or
patties but is more commonly used as animal feed. It can
even be further processed into soy yarn!
Storage and Shelf Life
Typical fresh tofu has a shelf life of 70 days from the date of
manufacture, if the package is unopened and refrigerated.
This type of tofu is pasteurized to prevent spoilage.
Once the package is opened, any leftover tofu should be
transferred to an airtight container and submerged under
fresh water, changed daily or every other day, and refriger-
ated. Following this procedure, tofu lasts 3 to 5 days.
If the unopened fresh tofu is spoiled, the package may
become bloated and puffy. If the water is cloudy and the tofu
has a sour smell or taste, it should not be consumed. Cloudy
or murky water alone is not a sign of spoilage.
Tofu can be frozen for up to 3 months; however, freezing
result in darker, spongier tofu. Dried tofu is typically shelf
stable, and freeze‐dried tofu has a significantly longer shelf
life than fresh, typically around 1 year.
Nutritional Profile
Tofu contains over 30 percent of the USDA recommended
daily value for calcium and iron and contains nearly twice as
much protein (8 g protein per 3½‐ounce [100 g] serving) as it
does fat.20 Tofu also contains over 20 mg soy isoflavones per
3½‐ounce (100 g) serving.21
Tempeh and Other Fermented
Soy Products
Tempeh is a mold‐fermented soybean cake or loaf made from
dehulled and cooked soybeans (traditionally soybeans only;
modern varieties may include brown rice, other grains such as
barley or millet, and even flaxseeds or bits of vegetables) fer-
mented with a Rhizopus mold. Tempeh originated in Indonesia
but has gained more widespread recognition in the West as soy
and fermented foods have gained in popularity. Tempeh has a
grainy, nutty, almost mushroom flavor and patty‐like texture,
with visible beans and grains joined together by a web of edible
mold. Tempeh is considered a whole‐food form of soy, as it
contains the entire soybean, and it is acclaimed for the bio‐
availability of nutrients released in the fermentation process.
Traditionally, the fresh soybeans are soaked overnight,
par‐cooked, cooled, mixed with a portion of previously made
tempeh to serve as starter, and then wrapped in a large
banana leaf to maintain humidity and provide exposure to
oxygen. Indonesia’s climate provides the appropriate tem-
perature and humidity for the molds to grow and transform
the soybeans into tempeh within 24 hours.
In the commercial process, dehulled soybeans are soaked
overnight, cooked for about 30 minutes, cooled, and mixed
with a tempeh starter such as Rhizopus oligosporus or
Rhizopus oryzae. Other mixed cultures containing both
Rhizopus and bacterial components such as Klebsiella species
may be used in order to generate higher levels of vitamin B12,
which is normally not found in vegetables.22 After 24 hours of
incubation (temperature should be in the 90s °F [33 or so °C]
with high humidity), the starter culture creates a web‐like
mycelium that binds the beans into a patty of fresh tempeh.
Storage and Shelf Life
Tempeh has a shelf life of 80 days in sealed packaging under
refrigeration.23 Due to the fermentation process, tempeh has
a high content of live bacteria, mold, and yeast, but under
proper fermentation these microorganisms are not harmful.
That said, tempeh is never eaten raw. After opening, tempeh
should be used in 3 to 4 days to avoid spoilage as the micro-
organisms continue to grow. Tempeh may exhibit a black-
ened or white fuzzy color and texture, which is normal to the
product and not indicative of spoilage. High levels of mold
growth, slimy texture, or off odors, however, may indicate
spoilage.24 Tempeh freezes well in sealed containers for up to
one year with no apparent change in texture or flavor.
Preparation and Cooking Methods
Tempeh maintains its dense, beany texture and distinct fla-
vor if sliced raw and dry‐cooked (pan‐seared, fried, grilled,
etc.). This texture and flavor is somewhat polarizing, appreci-
ated by some but off‐putting to others. To counteract this,
marinate tempeh overnight or simmer in a flavorful sauce for
an hour or so prior to cooking to loosen texture and infuse
flavor. Common marinades include light stock, soy sauce,
herbs, spices, wine, garlic, dried mushrooms, and smoke
c07.indd 113 1/14/2016 7:21:59 AM
114 • CULINOLOGY®
flavoring, among others. Tempeh can be baked in a casserole,
and cubed tempeh is an excellent addition to stews because
it absorbs the flavors of the cooking liquid.
Dry or marinated tempeh can be pan‐fried or deep fried
for crisp texture and golden color. Chunks of tempeh can be
pan‐ or deep fried into croutons or sliced into sticks and deep
fried to resemble French fries. Tempeh’s patty‐like shape can
be used in place of meat cutlets, and it works well marinated
and flour‐coated or traditionally breaded and then pan‐fried
(as a chicken cutlet). This style of tempeh can be made into
vegetarian marsala, française, bordelaise, and other classic
dishes by sautéing mushrooms or other ingredients and
deglazing the pan with the appropriate wine.
A very useful application is to grate tempeh on a coarse
cheese grater to mimic chopped meat. One can marinate,
season, and pan‐fry grated tempeh to make taco‐style meat,
vegetarian Bolognese sauce, and chili. The resulting crum-
bled texture is like that of the commercial frozen vegetarian
chopped meat analog typically made of TVP.
Nutritional Profile
Tempeh is considered less refined than other soy products,
such as tofu, as the whole soybean is retained. The fermenta-
tion process leads to significant nutritional changes to the
soybean. Tempeh is limiting in the amino acids methionine
and cysteine and is best served with rice to make a complete
protein. However, the mold in tempeh partially digests the
soybean proteins, liberating numerous amino acids, and
breaks down the trypsin inhibitors, making them more easily
digestible. This results in a higher protein efficiency ratio
(PER) than other soy foods. The fermentation process also
breaks down the oligosaccharide carbohydrates to make the
tempeh more digestible. The bacterial cultures used in tem-
peh also result in increased vitamin B12 content compared
with other vegetarian foods.25
Other Forms of Fermented Soy
Additional fermented forms of soybeans include miso, soy
sauce, and natto.
Miso is a paste of finely ground soybeans that often
includes barley and/or rice, salt, and a starter culture com-
posed primarily of the mold Aspergillus oryzae. This mixture
is allowed to ferment from 6 to 36 months, resulting in a
savory paste ranging in color from golden to reddish‐brown
to dark brown to black. Miso paste is rich in mouth‐filling
free glutamates, which give foods the meaty taste enhance-
ment known as umami. Commonly used to give depth and
flavor to soups, miso is also used to make sauces, marinades,
and salad dressings. High in salt, it is also rich in B vitamins
and protein.
Soy sauce is the most popular Asian condiment in the
world, including the United States. It is an ancient condi-
ment; its origin dates back to China, 200 bc. It is used in
countless commercially manufactured foods, even non‐Asian
ones, for its salty tang and flavor enhancement of soups,
sauces, dressings, marinades, and so on. It is made primarily
from soybeans, but traditional recipes use roasted wheat as
well. Soy sauce undergoes a similar fermentation to miso,
with Aspergillus oryzae as the primary organism involved,
although lactic acid bacteria and alcohol‐producing yeasts
get involved over time as well to provide their important fla-
vor characteristics to the sauce. The process takes several
months. At the end of the fermentation period the paste,
unlike miso, is filtered to separate the liquid soy sauce from
the press cake, which is used as animal feed. The sauce is
then pasteurized and bottled. Typically, soy sauce contains
14 to 18 percent salt, although reduced‐salt varieties are
available. In addition, a reduced‐color soy sauce can be used
in applications requiring a lighter finished product.
Note: In addition to the traditional fermented soy sauce,
there are also some brands of soy sauce that are made from
acid‐hydrolyzed soy protein instead of brewed with a tradi-
tional culture. This takes about three days. Although they
have a different flavor, aroma, and texture when compared to
brewed soy sauces, they have a longer shelf life and are usu-
ally made for this reason. The clear plastic packets of dark
sauce common with Chinese‐style take‐out food typically use
a hydrolyzed vegetable protein formula.
Natto is the term for whole cooked soybeans fermented with
Bacillus subtilis, resulting in a slimy, ammoniac, and pungent
product that is often served with rice as a breakfast food in
Japan. It is not very popular in the United States (outside of
Asian communities) due to its extreme sensory qualities.
Other Forms of Unfermented Soy
Soy protein isolate (SPI) is a highly refined or purified form of
soy protein with a minimum protein content of 90 percent on
a dry basis. It is made from defatted soy flour from which
most of the non‐protein components, fats, and carbohydrates
are removed. Soy isolates are mainly used to improve the tex-
ture of meat products but are also used to increase protein
content, enhance moisture retention, and as an emulsifier.
Flavor can be affected, but overall impact depends on usage
level. Food uses include protein supplementation and emul-
sification in beverage powders, infant formulas, liquid nutri-
tional meals, and some varieties of liquid soymilk; enhanced
moisture retention in meat and poultry marinades; nutri-
tional enhancement in cereals and baked goods; and the pri-
mary protein source in power bars. It is the most expensive
(and most concentrated) form of soy protein.
Soy protein concentrate (SPC) is about 70 percent soy
protein and is basically defatted soy flour without the water‐
soluble carbohydrates. Unlike SPI, it is made by removing
part, but not all, of the carbohydrates (soluble sugars) from
dehulled and defatted soybeans. SPC retains most of the fiber
of the original soybean. It is widely used as a functional or
nutritional ingredient in a wide variety of food products,
mainly baked foods, breakfast cereals, and some meat prod-
ucts. SPC is used in meat and poultry products to increase
water and fat retention and to improve nutritional values
(more protein, less fat). Soy protein concentrates are avail-
able as granules, flour, and spray‐dried powders.
c07.indd 114 1/14/2016 7:21:59 AM
CHAPTER 7 • Protein‐Based Foods: Vegetable Sources of Protein and Protein Complementation • 115
Soy flour is made by grinding soybeans into a fine powder.
It comes in several forms: full‐fat; defatted (oils removed);
with 50 percent protein content and with either high water
solubility or low water solubility; and lecithinated (lecithin
added for additional emulsifying properties). Soy flour is
gluten‐free and can be used in gluten‐free baked goods, but
gluten‐free breads made exclusively with soy flour are very
dense in texture. In addition, soy flour is the basic ingredient
used in the manufacture of textured vegetable protein.
Soy grits are made from soybeans that have been toasted
and cracked into coarse pieces. They may be stirred into
soups, stews, or casseroles, toasted with granola, or mixed
into ground beef or other proteins to make meatballs and
meatloaves. They can also be added to baked goods to pro-
vide texture and enhanced nutrition, and can be eaten like
cereal with milk and fruit.
Textured Vegetable Protein (TVP®)
Textured vegetable protein (TVP) is a refined soy food
made from soy concentrate or defatted soy flour and water.
TVP is a trademarked name of Archer Daniels Midland Co.
(ADM), which invented it. It is a spongy product that can
be extruded into various shapes and textures to mimic
meat, thereby providing a vegetarian and low‐cost substi-
tute for many meat products. It may also be called textured
soy protein (TSP).
Soy flour is defatted to remove oils and mixed with vary-
ing ratios of water to create a pliable vegetable protein dough.
This dough can be extruded in the presence of steam and cut
into shapes such as flakes, nuggets, patties, ground bits, and
slices. These can then be flavored with natural or artificial,
vegetable‐derived beef or chicken flavors and combined with
water or other liquid and further cooked to yield meat‐like
analogs. TVP is also made as a byproduct of the soybean oil
industry.26
Ingredients
Plain TVP typically has just one ingredient: soy flour. However,
it may also contain natural and artificial flavorings (such as
chicken, beef, turkey, bacon, sausage, pepperoni, or ham fla-
vor) when processed and sold to resemble a particular meat
product. More highly processed versions of TVP may include
added soy protein isolate or other proteins, such as wheat
gluten.
Storage and Shelf Life
Dry (non‐rehydrated) TVP has a minimum shelf life of one
year if stored in a sealed bag or container at room tempera-
ture. Some products claim a shelf life of two years from manu-
facture. As TVP is made from defatted flour, rancidity is not a
major concern in storage. Moisture absorption can be con-
trolled by ensuring the product is hermetically sealed. Once
rehydrated in a recipe, TVP shelf life is greatly reduced to less
than one week under refrigeration, depending on the other
ingredients, unless further processed (canned, for example)
or frozen.
Preparation and Cooking Methods
TVP is available in various sizes, including basic flakes, crum-
bles, and larger cubed chunks and strips. It must be rehy-
drated prior to or during cooking, most efficiently in boiling
water or flavored liquid (stock, broth, or other sauce). The
specific ratio of hot liquid to TVP depends on the particle
size; it varies from 1:1 for softer textured product to 0.75:1 or
0.5:1 for a tougher, denser texture. TVP typically rehydrates
within 2 to 10 minutes of soaking in boiling liquid, depending
on particle size.
Some small‐flake bacon‐flavored TVP (Bac‐Os®) can be
sprinkled on salads without rehydration. The smallest granu-
lated TVP can be added without rehydrating directly into liq-
uid stocks, broths, soups, and stews to cook along with other
ingredients. Larger chunks of TVP should be partially rehy-
drated with water and then cooked further (typically 5 to 10
minutes or more) directly in a liquid‐containing recipe. If not
pre‐hydrated, these larger chunk‐type products must be
cooked for longer periods in soups and stews.
The texture of the cooked TVP is a good indicator of done-
ness. Adding acid to TVP loosens texture and speeds up
re hydration.27 TVP in crumble or small‐flake form can make
vegetarian versions of taco filling, chili, sloppy joes, and
Bolognese sauce, and may be blended with bread crumbs and
eggs to form burgers, meatballs, or meat loaf. Chunk‐style TVP
can make vegetarian stews, kebabs, satays, and stir‐fries.
Nutritional Profile
TVP is an excellent source of protein, with 50 g protein per
3½‐ounce (100 g) serving before rehydration. It also provides
18 percent of the RDV of dietary fiber and is low in fat due to
the oil removal from the soy flour.28
New‐Generation Soy Foods
Vegetarian diets have grown in popularity throughout the
United States, and the functionality of soy foods has been well
advertised in recent years. In addition, awareness of food
allergies or intolerances to dairy products has risen in the
mainstream marketplace. This combination of trends has led
to a new generation of soy foods that mimic meat and dairy
products. These analogs fill a void in the vegetarian diet and
can provide a cholesterol‐free and lower‐fat option even to
non‐vegetarians who simply want to eat healthier. However,
unlike the soy foods previously described, this new genera-
tion of soy foods is often highly processed and refined with
many ingredients, additives, and processing aids to mimic the
flavor and texture of the meat and dairy items they replace.
Types and Styles of New‐Generation Soy Foods
Soy versions of sliced and grated cheese, soy‐based versions
of ice cream and yogurt, breakfast sausage, cold cuts,
Thanksgiving turkey, buffalo wings, chicken nuggets, pep-
peroni, chopped meat, ribs, hot dogs, bacon, and burgers can
be found in most major supermarkets and health food stores.
Combination items such as frozen pizza with soy cheese and
soy pepperoni can be found in the freezer section. Soy‐based
c07.indd 115 1/14/2016 7:21:59 AM
116 • CULINOLOGY®
sports and snack bars have gained in popularity due to their
ability to pack a high serving of protein in a small package.
Major U.S. brands include Lightlife, Morningstar Farms, Boca,
Dr. Praeger, Amy’s Organics, Vegan Gourmet, and Tofutti.
Production methods often involve blending soy flour,
other vegetable proteins, flavorings, texturizing agents, and
colors, and molding, forming, or extruding the finished prod-
uct under heat and pressure to resemble meat or cheese.
Ingredients
A look at the ingredient panel of many new‐generation soy
foods tells the trained food scientist or nutritionist that these
are not necessarily health foods. The multitude of ingredients
and processing aids in some of these products can resemble
the list commonly found on a snack or junk food item. Many
of these foods not only contain a soy protein base but also
wheat gluten (also an allergen under FALCPA) as well as
many other starches, fillers, and flavorings.
New soy foods can have higher protein contents than
traditional soy foods due to the addition of more concentrated
protein isolates as well as added wheat proteins. However,
these refined ingredients may present greater challenges for
digestion. It is important to note that some new soy foods also
contain wheat and even dairy‐derived ingredients (non‐
vegan) as well as potentially sensitizing ingredients such as
monosodium glutamate (MSG), and these must be noted on
all labels for manufactured products.
Grains
Cereal grains make up half of the top 18 crops grown around
the world, with wheat, maize (corn), rice, and barley taking
the top four spots, responsible for nearly three times the annual
volume of all the other crops combined.
Grains have been part of the traditional diets of both ancient
and modern cultures for thousands of years, and especially
important in cultures where the availability of animal proteins
is limited. Their limiting lysine content can be complemented
by legumes to make a complete protein. Although rice and
beans come to mind as an example of such a complementary
pairing, several grains (quinoa, buckwheat, amaranth) have a
very high protein and lysine content. It is important to note
that whole grains (with the germ, bran, and endosperm intact)
have significantly higher protein, fiber, and vitamin content
than refined grains (which are proportionately higher in
starch). For example, wheat processed into white flour has 10 g
protein and 2.7 g dietary fiber per 3½‐ounce (100 g) serving,
while whole wheat flour contains 13.7 g protein and 12.2 g
dietary fiber per 3½‐ounce (100 g) serving.29
The preference of the U.S. public for refined white flour
products (white bread, crackers, pasta) and resulting nutri-
tional deficiencies (especially in infants and children) by the
1920s led to research on nutrient losses in processing. Thus,
the idea of enrichment, or adding back the most essential
nutrients lost in processing, was born. Thiamin, riboflavin, nia-
cin, folic acid, iron, and even calcium have been commercially
FIGURE 7.2 Protein in soy products.
Product
Serving
Size
Protein
(grams)
Tofu—medium to extra firm 3 oz. 7 to 12
Tofu—soft or silken 3 oz. 4 to 6
Tempeh 4 oz. 12 to 20
Textured vegetable protein (TVP) ¼ cup 10 to 12
Source: USDA National Nutrient Database for Standard Reference,
http://www.nal.usda.gov/fnic/foodcomp/search.
Wheat
Maize
Rice
Barley
Soybean
Cane sugar
Sorghum
Potato
Oats
Cassava
Sweet potato
Beet sugar
Rye
Millets
Rapeseed
Bean
Peanut
Pea
468
429
330
160
67
60
54
43
41
35
34
29
26
19
14
13
12
Million Metric Tons
0 100 200 300 400 500
88
FIGURE 7.3 The most commonly grown crops in the world.
Source: Adapted from University of Wisconsin Alternative Field Crops Manual, 2000, and USDA Standard Reference.
c07.indd 116 1/14/2016 7:21:59 AM
http://www.nal.usda.gov/fnic/foodcomp/search
CHAPTER 7 • Protein‐Based Foods: Vegetable Sources of Protein and Protein Complementation • 117
FIGURE 7.4 Protein in grains (cooked).
Grain (1 cup) Protein (grams)
Amaranth 7
Barley, pearled 4 to 5
Barley, flakes 4
Buckwheat groats 5 to 6
Cornmeal (fine grind) 3
Cornmeal (polenta, coarse) 3
Millet, hulled 8.4
Oat groats 6
Oat, bran 7
Quinoa 5
Rice, brown 3 to 5
Rice, white 4
Rice, wild 7
Rye, berries 7
Rye, flakes 6
Spelt, berries 5
Teff 6
Triticale 25
Wheat, whole berries 6 to 9
Couscous, whole wheat 6
Wheat, bulgur 5 to 6
Source: USDA National Nutrient Database for Standard Reference,
http://www.nal.usda.gov/fnic/foodcomp/search.
added to white flour since the 1940s. Interestingly, there was
no widespread effort to return proteins and amino acids lost in
processing of grains because grains were never seen as pri-
mary sources of protein in the U.S. diet.
Whole Grain–Enhanced Products
Over the years, the comparative health benefits of whole ver-
sus refined or processed grains has been the subject of signifi-
cant research, which has led to an enormous increase in
product development, marketing, and sales of whole‐grain
products. The focus on whole foods and whole grains has
gained enormous awareness and popularity along with vege-
tarian diets. The use of the claim “contains whole grains” is a
powerful marketing tool for virtually every grain‐based food
on the supermarket shelf: breads and rolls, waffles, pasta,
crackers, cereals, pizza dough, muffins, sports bars and
granola bars, rice dishes, baking mixes and flours, snack
chips, cookies, and cakes. Even beverages, soups, and dairy
products can contain soluble fiber extracted from grains.
An industry association, the Whole Grains Council, helps
consumers identify foods that are good sources of whole
grains by awarding special stamps that can be placed on the
packaging of qualifying products. Of course, the presence of
a Whole Grain Stamp on a food package doesn’t guarantee
the healthfulness of the food inside; even foods high in fat,
sodium, and sugar (such as highly sweetened breakfast
ce reals) can carry the stamp as long as they are made from
whole grains. In addition, the Council provides a list of res-
taurants that offer at least one whole‐grain offering at every
meal, a list of bulk vendors of whole‐grain products for food
manufacturers and chain restaurants, and a list of private‐
label manufacturers specializing in the development and
manufacturing of whole‐grain foods.
The USDA’s Dietary Guidelines for Americans recommends
eating at least 3 ounces (85 g) of whole‐grain products per day,
with the rest of the recommended daily intake (if not from
additional whole grains) coming from refined grain products
(breads, pasta, cereals, etc.) that have been enriched with B
vitamins and iron. Further, individuals should “[c]onsume at
least half of all grains as whole grains [and] increase whole‐
grain intake by replacing refined grains with whole grains.”30
U.S. consumers have begun embracing whole grains for
health; however, the taste and texture of whole‐grain products
can differ significantly from the highly processed white‐flour
counterparts to which they are accustomed. Thus, blended, or
“made with whole grains” products have come to the forefront
of the market, combining traditional white flour with whole
wheat flour or a combination of wheat germ, wheat gluten,
wheat bran, or other grain components. In the past, the card-
board color, texture, and taste of whole wheat pastas, cereals,
and baked goods frightened away all but the healthiest‐minded
eater, and much formulation and marketing has gone into
improving the palatability and image of whole‐grain and whole
grain–enhanced products. One might think of them as “gate-
way foods” that prepare the consumer palate for the texture
and flavor of 100 percent whole‐grain products.
The FDA has reinforced the message of whole grains and
health, and many companies have jumped on the band-
wagon. The largest breakfast cereal manufacturers now
proudly tout that their cereals are made with whole grains.
Even many traditional white breads now have added wheat
protein and fiber. Hybrid pastas are made of white flour with
added protein, fiber, and even omega‐3 oils in varying ratios.
Supermarket shelves teem with 100 percent whole wheat
pastas, including many private‐label versions. Some well‐
known crackers and snack crisps are now available with
some amount of whole wheat flour or added protein or fiber.
Many commercial ground‐meat products contain oat fiber or
oatmeal to reduce calories and increase dietary fiber.
Initially, the key to making whole grain–enhanced prod-
ucts a success in the mainstream marketplace was to hide
the flavor, color, and texture of the whole grain. Finely milled
flours, innovative fiber extracts, and various forms of grain
proteins were formulated to blend seamlessly with white‐
flour products.
c07.indd 117 1/14/2016 7:21:59 AM
http://www.nal.usda.gov/fnic/foodcomp/search
118 • CULINOLOGY®
FIGURE 7.5 Grain characteristics.
Name of Grain Grain Characteristics
Amaranth Ancient Aztec grain. Nutty, slightly spicy and sticky, gelatinous texture. High in fiber and protein.
A superfood with superior protein quality.
Barley Pearled barley is lightly milled or refined and is mild flavored and chewy. Whole barley is very chewy,
retaining the whole grain. Barley is used in soups, stews, and pilafs, and is the primary grain used in the
manufacture of beer and whiskey via the “malting” process.
Buckwheat
groats
Also called kasha. Roasted with a nutty flavor. Generally, is first coated with beaten egg and cooked
over heat to avoid mushiness. Delicious as pilaf.
Bulghur wheat Wheat berries that have been steamed, dried, and cracked. It cooks quickly. Used to make the Middle
Eastern specialty tabbouleh.
Corn A New World food, corn is one of the most important grains in human nutrition. Very versatile; eaten
from the cob as a vegetable, and used to make polenta, desserts, animal feed. Corn starch, corn syrup,
and corn oil are other important staples.
Couscous A traditional pasta‐like food of North Africa, couscous is made from ground semolina, which is mixed
with water, then steamed, sieved, and dried. Traditionally cooked in a couscoussière. Good in pilafs, in
salads, or combined with vegetables.
Farro Ancient grain in the wheat family from North Africa and the Middle East. A staple during the Roman
Empire. Common in Italy. Also known as emmer or by its scientific name, triticum dioccum. Complex,
nutty taste. Creamy like risotto.
Hominy Dried white or yellow corn kernels, hull and germ removed by soaking in slaked lime or lye. Commonly
served as a side dish or part of a casserole (Mexican posole). When ground, is referred to as grits. These
are usually simmered in water or milk until thick. Can be served in this form or chilled, cut, and fried.
Kamut An ancient Egyptian wheat, kamut has a rich, buttery flavor and chewy texture. Is a good wheat substi-
tute for allergy sufferers, but not for people with celiac disease as it still contains gluten.
Millet Believed to have been the staple grain of the Chinese before rice, millet is now grown throughout the
world, although in the United States its primary use is as birdseed. Millet is tiny, gluten free, and easy
to digest. Boil like rice, or cook in soups and stews.
Oats A major U.S. crop, oats have an inedible hull, which is removed, leaving the remaining grain, called the
groat, with bran and germ layers intact. Available pre‐steamed and rolled as “instant” rolled oats, or
chopped as Irish or steel cut oats.
Polenta A cornmeal mush that is a staple of Northern Italy. Can be eaten hot or cooled until firm, then cut and
fried.
Quinoa This tiny, disk‐shaped seed (pronounced “keen‐wah”) is an ancient grain now being recultivated in
South America. It is known as the only grain with a complete set of essential amino acids. It may be
boiled like rice and used in soups and stews.
Rice Cultivated since at least 5000 B.C. It is the main food source for almost half the world’s population, pre-
dominantly India, Indonesia, China, Japan, and Southeastern Asia. There are more than 2,500 varieties
grown. Because it requires vast amounts of water to grow, rice is most suited to tropical regions.
Rye An important grain in central and eastern European cuisines. Used for baking hearty dark breads and
some local specialties. Rye grows well in more hardy climates. Rye produces a very weak gluten net-
work, resulting in very dense dough.
Spelt A wheat variety more than 9,000 years old, brought to this country by the Amish. Has a light, nutty
flavor. Spelt is easier to digest than other wheat varieties, but contains gluten, making it inappropriate
for celiac disease sufferers. Used in pilafs or salads.
Sorghum Similar to corn, sorghum is one of the top cereal crops in the world, next to wheat, oats, and corn.
Originating in Africa, it is now grown in the United States as well as Asia. Used for animal feed, starch
production, or porridges.
Teff The smallest grain in the world, native to northern Africa. Mild nutty flavor.
Triticale Developed in the late 1800s, triticale is a cross between rye and wheat. Nutty flavor. Great in pilafs,
used in soups or stews.
c07.indd 118 1/14/2016 7:21:59 AM
CHAPTER 7 • Protein‐Based Foods: Vegetable Sources of Protein and Protein Complementation • 119
It is important to note that “made with whole grains” differs
from “100 percent whole grain,” and some companies use the
public’s lack of understanding to convince consumers that
they are buying less refined products. Several companies have
received negative public and media attention by advertising
“whole‐grain goodness” while a majority of their product is
made from white flour. Removing and then adding back nutri-
ents in other forms can be, at the very least, misleading to
untrained consumers, but savvier health consumers look for
foods as close to a whole‐food source of grains as possible.
The FDA has posted industry guidance on their website to
assist in labeling whole‐grain and whole grain–enhanced
products as well as accepted health claims.
Rice
Rice is the seed of the plant Oryza sativa, and it is arguably
the most important grain grown to support human nutrition:
it provides more than one‐fifth of the calories consumed
throughout the world.31 It is important to note that more
than half of the world’s population depends on rice as their
primary food, especially in the Far East, where people eat it
three times a day. China and India are the world’s major pro-
ducers. Rice, like almost all grains, is limiting in lysine, which
can easily be supplied by complementation with legumes.
That said, rice has the highest PDCAAS score of any grain:
0.86, where the top score is 1.0 (egg white, milk proteins, and
soy protein). Brown rice is much better nutritionally than
polished white, even though white is much more popular in
the United States. In fact, a diet consisting mainly of white
rice results in beriberi, a deficiency in vitamin B1 (thiamine).
Depending on the type of beriberi (wet or dry), symptoms
may include loss of muscle function (especially in the lower
legs), numbness of the extremities, shortness of breath, swell-
ing of the lower legs, mental confusion and difficulty speak-
ing, pain, vomiting, and paralysis. Beriberi is quite rare in
developed countries, including the United States, where
many foods are enriched with thiamine.
Rice is a semiaquatic grass typically grown in regions with
high rainfall as harvesting is assisted by flooding, which
causes the small, edible seeds to float. The name wild rice is
often applied to Zizania plants, which are technically a sepa-
rate reed‐type grass unrelated to rice.
Rice in its whole‐grain form (with the bran intact) is
brown. Removing the bran (pearling) yields white rice. This
processing removes some nutritional value, so brown rice is
higher in protein, vitamins, minerals, and fiber than white
rice. Because of this, enrichment of white rice with vitamin B1,
vitamin B3, and iron is required by law in the United States.
Both forms of rice can be processed into “converted rice” by
parboiling. This helps force the nutrients into the endosperm
of the rice before the bran is removed. Converted or parboiled
rice retains more nutritional content but takes a bit longer to
cook than traditional white rice. Rice can be fully cooked and
then flash frozen and dried into instant rice, which retains
the least nutrients but is the quickest cooking.
Rice is classified by its size—long grain, medium grain, or
short grain—and each variety has different cooking methods
and resulting textural properties in a dish. Long‐grain rice tends
to remain somewhat firm and separate when cooked, with a
fluffy texture. Short‐grain rice has more starch and is sticky or
creamy when cooked. Medium‐grain rice has characteristics of
both and can be either fluffy and distinct or sticky, depending
on cooking time and how long it sits after cooking.
Typically, rice is cooked at a ratio of 2 parts liquid to 1 part
rice. The most common method is to bring the liquid to a boil
and simmer the rice, covered, for the recommended time
(from 15 to 60 minutes, depending on type). Some types of
rice require rinsing, soaking, and pre‐sautéing in oil (pilaf
method) or adding hot liquid slowly while stirring continu-
ously (risotto method).
For the pilaf method, before the grain is cooked it is
toasted in a hot pan either dry or coated with a small amount
of fat. This technique, known as parching, alters the flavor,
creates a firmer texture, and helps keep the grains separate
during the subsequent cooking. In addition, the dextriniza-
tion of the starches caused by the dry heat during parching
makes them more easily digestible.
A carefully measured amount of water, stock, or similar liq-
uid is added to the parched grain and brought to a boil. Then
the rice, covered, is simmered on the stove or in the oven
(oven cooking allows for more even heating). It is important
to use the appropriate amount of liquid; too much results in a
mushy, porridge‐like consistency, whereas too little does not
fully hydrate the grains, causing the pilaf to remain hard no
matter how long it is cooked. In a properly prepared pilaf, the
individual grains separate easily and are tender to the bite,
with a noticeable texture. No matter which grain is used, the
method is the same. However, the ratio of grain to liquid and
the time necessary to cook the grain vary tremendously.
Name of Grain Grain Characteristics
Wild rice A grass seed native to the Great Lakes Region of the United States. Not actually a rice, it used to be a
very important staple food for Native American tribes in the northern part of the country. Distinct nutty
flavor; cooked like rice pilaf with a much longer cooking time.
Wheat berries Whole wheat berries can be sprouted and used in salads or pilafs. Pre‐cooked, dehydrated, and
cracked wheat berries known as bulgur are a great substitute for whole wheat berries.
Source: Hinnerk von Bargen.
c07.indd 119 1/14/2016 7:21:59 AM
120 • CULINOLOGY®
For the risotto method, short‐grain rice is constantly
stirred during cooking, and liquid is added in stages. The
constant stirring causes some of the starch to ooze out of the
rice grain, resulting in a very creamy consistency. A properly
prepared risotto has an almost pourable consistency, yet the
individual grains retain a noticeable texture referred to as
al dente (“to the tooth”). It is important to understand, how-
ever, that not all kinds of short‐grain rice are suitable for
risotto. Japanese short‐grain rice, perfect for sushi, turns into
an unpleasant mush if it is stirred during cooking.
In the culinary culture of many Asian countries, sticky rice
is a popular dish. This should not be confused with regular
steamed rice served as a side dish. Steamed rice just happens
to be sticky to one degree or another, as the grains were not
parched before the liquid was added. Genuine sticky rice,
available in short‐ and long‐grain varieties, is also known as
glutinous rice. After cooking, it becomes especially sticky; it is
commonly used for sweet dishes. The raw glutinous rice
grains are easily recognized; they are much whiter and more
opaque than other white rice varieties.
Here are some commonly available types of rice:
• Long grain: brown rice, white rice, parboiled or con-
verted rice, and aromatic varieties such as basmati, jas-
mine, Jasmati®, and Texmati®, which are prized for their
nutty, popcorn‐like aroma and flavor.
• Medium grain: River, Calrose, and Goya are popular
brands.
• Short grain: Italian arborio and carnaroli varieties, which
are commonly used in risotto, and sticky rice, which is
common to Asian cuisines and also good for puddings.
• Exotics: wild rice, wild pecan rice, black or japonica rice,
and other colored rices, such as Bhutanese red rice and
Thai purple.
Rice may be ground into flour for use in baking or product
formulation (particularly in gluten‐free applications), and
soaked and processed into rice milk to provide a dairy‐free
milk alternative. Other rice products include rice syrup
(made by hydrolyzing rice starch), rice bran, puffed rice cakes,
and cereals. Rice starch is commonly used in commercial
meat marinades to help muscle fibers bind water.
Corn
Corn is the largest field crop in both volume and cash value in
the United States, more than double any other crop. Outside
the United States, corn is called maize. Although corn is con-
sidered a vegetable by some, its nutritional function is that of
a grain. It is available fresh on the cob; frozen; canned (in brine
or creamed); dried as popcorn (with different colors available,
such as red and black); processed with alkali (nixtamalization)
to make masa, masa harina, and hominy; dried and ground
into cornmeal or corn flour; further processed into tortillas,
tortilla chips, and corn chips; baked into muffins and corn-
bread; and cooked into porridge or polenta. Corn is also made
into oil, syrup, high‐fructose syrup, popcorn cakes, and cere-
als. In spite of all of these food applications, most corn is grown
for animal feed and, to some extent, ethanol. Only 1 percent of
the corn grown is actually consumed as corn.
The freshest corn requires very little cooking and has a
sweet flavor. The kernels may be yellow or white, or even
mixed on the same cob (butter-and-sugar corn). Most mod-
ern corn cultivars grown for human consumption have been
bred to be supersweet. Cooking methods for fresh corn
include grilling, boiling, or microwaving on the cob; frozen
corn is commonly microwaved or gently sautéed or grilled
in a cast‐iron pan. Canned corn is cooked in the canning
process and needs only to be warmed on the stovetop or in a
microwave. Popcorn may be oil or air popped. Corn flakes
and corn pops are both among the oldest forms of ready‐to‐
eat cereals. Cornmeal imparts a dry, crumbly texture to
dough and may be blended with eggs, water, or other binding
agents and leavening to make cornbread or muffins. Masa
and masa harina are used in traditional Central American
cooking and are useful for finer‐grained foods such as tamale
filling or dumplings. Cornmeal breading is also commonly
used, especially in Southern foods such as fried okra, fried
catfish, and fried pickles. And traditional Southern “cornmeal
mush” is virtually the same thing as Italian polenta.
Wheat and Derivatives
Wheat refers to any species of the genus Triticum. More
wheat is now produced around the world than any other
grain. About 75 percent of all harvested wheat is made into
flour; the remaining 25 percent goes into cereals, pasta,
wheat germ and wheat germ oil, and animal feed. The two
major types of wheat are winter wheat (hard) and spring
wheat (soft). Hard wheat has a higher protein (gluten)
content, making it better for breads and pasta. Soft wheat is
lower in protein, making it better for cakes and pastries.
Wheat is available as whole wheat berries, cracked
(groats), bulgur (parboiled and dried), farina (cream of
wheat), whole wheat flour, white flour, bread flour (high
protein/gluten), durum flour, all‐purpose flour, cake flour,
self‐rising flour, bran, germ, and vital wheat gluten, among
other forms. Wheat flour is commonly extruded with water
into pasta, noodles, and cereals; it provides the foundation
for cakes and cookies; and its high gluten content allows it to
make bread doughs that rise (with the help of a leavening
agent). Flour is also used to dredge raw meats, poultry, and
seafood before sautéing or frying, as a breading ingredient to
improve adhesion, as a nonstick agent when kneading
doughs, and for dusting the interior of a cake pan (together
with butter or oil) prior to filling with cake batter.
Flour is not only used for baking; after fat, all‐purpose flour
is the second ingredient in roux, a binder used to thicken gra-
vies, cream soups, and similar preparations. For a roux, all‐
purpose flour is cooked in a measured amount of fat for a
certain period before it is combined with the liquid it is meant
to thicken. The application of dry heat during cooking is a
crucial step because it causes the starch to dextrinate—that
is, the starch chains break into shorter units. This results in
the typical velvety mouthfeel of roux‐thickened sauces.
c07.indd 120 1/14/2016 7:22:00 AM
CHAPTER 7 • Protein‐Based Foods: Vegetable Sources of Protein and Protein Complementation • 121
The shortened starch chains are not able to bind as much
water, which results in the diminished thickening power of
roux versus raw flour. In fact, the longer a roux is cooked
before it is added to the liquid being thickened, the less thick-
ening power it retains. To achieve the same amount of thick-
ening in a sauce, a larger amount of dark roux (produced by
longer cooking) than light roux is necessary to get the same
viscosity. In addition to mouthfeel and texture, flavor and
color are also affected by roux. Caramelization and the
Maillard reaction caused by heating the flour in fat has a great
impact on the sauce’s color and flavor; the longer the roux is
cooked prior to addition to a sauce, the darker and stronger
the caramelic and toasted notes that develop, and these
flavors and colors are transferred to the thickened sauce.
The cooking methods for wheat and related grains are as
varied as its many forms; they range from soaking wheat ber-
ries overnight and simmering them (much like beans) to
combining flour with yeast, water, and other ingredients to
make dough.
Wheat contains glutenin and gliadin—proteins that form
an elastic, non‐water–soluble matrix of protein called gluten
when wheat flour is hydrated and then kneaded into dough.
Glutenin and gliadin make up 80 percent of the protein in
wheat flour. The sulfur atoms in the glutenin portion of the
flour allow the proteins to cross‐link.32 The amount of protein
in the starting flour, along with the amount of kneading, influ-
ences the development of gluten and thence the texture in
baked goods. Tender baked goods are best obtained using
cake flour (low in gluten), with minimal mixing. Tougher,
chewier products, such as bagels, are made using flour with
higher protein content (or even with added gluten), added
water, and additional kneading. The gluten matrix traps car-
bon dioxide in yeast‐leavened doughs, allowing the bread to
rise. Reduction of water, less working or kneading, and blend-
ing in fat or shortening can interfere with cross‐linking and
result in a tenderer, flakier product such as pie crust.
Note: The FDA has declared wheat (and wheat products) as
one of the major eight allergens, according to FALCPA.
Therefore, any product that contains wheat or wheat protein
derivatives must be clearly labeled as such in the ingredient
statement or in a comprehensive “contains” statement after the
ingredient list. As of 2013, the FDA has also defined gluten‐free
as a term used on finished product labels. Per the FDA:
Gluten‐free foods must contain less than 20 parts per million
(ppm) gluten. Foods may be labeled gluten‐free if they are
inherently gluten free; or do not contain an ingredient that is:
(1) a gluten‐containing grain (e.g., spelt wheat); (2) derived
from a gluten‐containing grain that has not been processed
to remove gluten (e.g., wheat flour); or (3) derived from a
gluten‐containing grain that has been processed to remove
gluten (e.g., wheat starch), if the use of that ingredient results
in the presence of 20 ppm or more gluten in the food.33
It is important to note for all grains discussed here,
although the grains themselves may be inherently gluten‐free,
many are grown and processed alongside gluten‐containing
grains and may contain gluten from cross‐contamination in
farming and milling. Certified gluten‐free grains are available
that have been tested to meet FDA standards for gluten‐free.
Before making any gluten‐free claim in formulation, it is
important to ensure that ingredients are sourced and tested
to meet this standard.
Awareness of celiac disease, also known as gluten intoler-
ance, is growing. Celiac disease is not classified as an aller-
genic response because it doesn’t trigger the release of
histamine or cause the release of IgE (immunoglobulin E)
antibodies. It does, however, cause chronic inflammation of
the villi in the intestine, interfering with absorption of nutri-
ents and creating gastrointestinal discomfort. Awareness of
the illness has reached large enough proportions to spawn
the creation of a plethora of gluten‐free foods, now available
even in mainstream supermarkets. The list of gluten‐free
replacements for foods normally made from wheat flour,
including breads and pastries, pastas and cereals, and cook-
ies and crackers, grows daily.
Barley
Barley is a species of the genus Hordeum, which contains glu-
ten. Barley is traditionally made into porridge, bread, and
beer and has much historical significance in Europe and the
Near East. Most of the barley crop today is used for animal
feed, but some is also processed and cooked in multiple ways
for human consumption. That said, most barley for human
consumption is used in the manufacture of beer (through the
malting process).
The first step in processing barley is dehulling, or removal
of the outer husk. Dehulled barley that has been steamed and
polished to remove the bran is called pearled barley. Barley
may be cooked (boiled and simmered 40 to 45 minutes) or
ground into flakes, meal, or flour and used in soups and
infant cereals. It can also be processed into a coffee‐like bev-
erage. Nutritionally, barley can assist in regulating blood
sugar34 and contains a favorable ratio of amino acids. In 2006,
the FDA authorized a health claim for the soluble fiber (beta
glucans) in whole‐grain barley. Based on research, the FDA
concluded that whole‐grain and dry‐milled barley products
providing at least 3 grams of beta‐glucan soluble fiber per day
are effective in lowering cholesterol (total blood cholesterol
and low‐density lipoproteins [LDL], the so‐called bad
cholesterol).
Oats
Oats are a cereal grain of the species Avena sativa and are not
considered a prohibited grain under the FDA’s gluten‐free
regulations, as research shows that most people with celiac
disease can tolerate oats (depending on their sensitivity).
Certified gluten‐free oats are available. Oats are grown and
processed for animal and human food and are available in
these forms:
• Whole‐grain groats (with only the husks removed) have the
densest texture and longest cooking time (they are typi-
cally brought to a boil and simmered at least 60 minutes).
c07.indd 121 1/14/2016 7:22:00 AM
122 • CULINOLOGY®
• Steel cut or Irish oats are groats cut into pieces. These
have a dense, chewy texture and a slightly shorter cook
time (30 to 45 minutes).
• Scottish oats are ground into a meal that cooks up in
about 30 minutes into a porridge‐type product with a
creamy mouthfeel.
• Rolled or old‐fashioned oats are steamed groats flattened
by a roller. Available in varying thicknesses, they make a
thick and chewy oatmeal cereal in about 15 minutes and
work well for granolas or to grind into oat flour. Quick‐
cooking oats are rolled even flatter to reduce cook time
to 3 to 5 minutes.
• Instant oats are also rolled thin, but are also cooked and
then dried again so they can be prepared instantly by
stirring hot water into the product.
Oatmeal acts as a meat extender in meat loaf and similar
products. Of course, it is also used in baked goods like the ever‐
popular oatmeal cookies. Oat flour is used as a thickener in
soups and may be used as a coating for commercially diced
and dried fruit for free flow (such as dates). Oat flour is also
used to make breakfast cereals. Old‐fashioned oats, toasted
and ground to a fine flour, can substitute for part of the all‐
purpose flour to produce interesting pancakes, breads, and
even pasta dough. Due to its lack of gluten‐forming proteins,
oat flour is best used in conjunction with wheat flour for use
in breads.
Sprouted oats are whole‐grain oats soaked in water to
sprout and enhance nutrient content. These are often added
to salads, breads, and other baked goods.
Oats have received a lot of attention for their health‐
promoting properties. In 1997, the FDA authorized state-
ments linking oat consumption to heart health after research
confirmed that the soluble fiber in oat bran (oat beta‐glu-
cans) lowers cholesterol and saturated fat. This has been
taken advantage of ever since by cereal companies anxious to
make health claims on their package labeling. Oat bran has
the highest content of beta‐glucan fiber, followed by rolled
oats and then oat flour. Oats have somewhat higher fat con-
tent than other grains and provide a good source of protein.
Other Grains
Rye is a wheat‐related species of the genus Secale and con-
tains gluten. It is most commonly used to make bread flour or
for animal feed rather than being cooked in whole form. Rye
is also processed and distilled into certain types of whiskey
and vodka.
Triticale is a cross between wheat and rye that contains
gluten. It was developed to combine the high yield potential of
wheat with the disease resistance and environmental toler-
ance of rye. This relatively recent hybrid typically is not cooked
whole but rather made into flour used in pastas and bread.
Buckwheat is not a true grain but due to its starch content
has similar properties. Botanically, it is a fruit related to rhubarb
and sorrel. Buckwheat’s triangular seeds do not contain gluten
and are used in many cuisines worldwide. Buckwheat groats are
cooked by boiling and then simmering. Buckwheat’s culinary
applications extend to breakfast porridges, kasha pilafs, and
noodle dishes, and the flour can be processed into Japanese
soba noodles and used in pancake mix. In Russia, buckwheat
blini (small pancakes) are traditionally served with caviar.
Buckwheat flowers are also used to make a unique honey.
Sorghum (also known as milo) is a member of the grass
family that is grown worldwide for animal feed, food ingredi-
ents, and fuel. It is considered gluten‐free. Sorghum is not
FIGURE 7.6 Grain‐to‐liquid ratio by volume.
Name of
the Grain
Grain-to-Liquid
Ratio by Volume
Approximate
Cooking Time
Amaranth 1:1½–2 20–30 minutes
Arborio rice (risotto) 1:3 20–30 minutes
Barley 1:2½ 50–60 minutes
Barley, pearled 1:2 35–45 minutes
Basmati rice 1:1¼ 18 minutes
Buckwheat 1:1½–2 12–20 minutes
Bulgur wheat, pilaf 1:2½ 15–20 minutes
Bulgur wheat, soft 1:3 30 minutes soaking
in boiling water
Converted rice 1:1¾ 18 minutes
Couscous 1:2 20–25 minutes
Hominy grits 1:4 25 minutes
Hominy, whole 1:2½ 2½–3 hours
Japanese short
grain
1:1¼ 18 minutes
Jasmine rice 1:1¼ 18–20 minutes
Kamut 1:3 1 hour
Long grain, brown
rice
1:3 45 minutes
Millet 1:2 30–35 minutes
Oat groats 1:2 45–60 minutes
Polenta/cornmeal 1:3–3½ 35–45 minutes
Quinoa 1:1 15 minutes
Short grain, brown
rice
1:2½ 35–40 minutes
Spelt 1:3 1 hour
Teff 1:3 15 minutes
Triticale 1:2½ 1 hour
Wheat berries 1:3 2 hours after
soaking
Wild rice 1:3 1 hour
Source: Hinnerk von Bargen.
c07.indd 122 1/14/2016 7:22:00 AM
CHAPTER 7 • Protein‐Based Foods: Vegetable Sources of Protein and Protein Complementation • 123
typically cooked whole but further processed into flour,
syrup, and other products. It may be fermented into alcoholic
beverages, particularly in Asia.
Bulgur is a mixture of several wheat species that have
been parboiled and dried, with a portion of the bran removed.
It contains gluten and appears similar to, yet differs from
cracked wheat due to a parboiling step, which reduces cook-
ing time. Bulgur may be added to boiling water and covered
and steam‐cooked for 5 to 10 minutes. It is common in
Middle Eastern recipes such as tabbouleh and bulgur pilaf.
Ancient Grains
Along with vegetarianism and healthy eating, previously
underappreciated whole grains such as quinoa, spelt, ama-
ranth, Kamut®, farro (emmer), and teff (also known as
“ancient grains”) are being rediscovered in the culinary
world. These grains can be prepared like other grains: whole
in pilafs and risottos, or ground into flour and used in baked
goods. The soaked and sprouted raw grains are eaten in sal-
ads or used in baking.
Certain ancient grains are considered a complete source
of protein (especially quinoa), and ancient wheat species
such as spelt and Kamut® are said to be more easily tolerated
by persons with wheat allergies despite their gluten content.
Quinoa is not technically a cereal grain or a member of the
grass family; it is the seed of the leafy plant Chenopodium qui-
noa. This small, flattened round seed originated in the Andes
and was prized as a sacred grain by the Incas. At present, qui-
noa is elevated to a high status due to its complete protein
content and suitability for vegetarian diets. Unlike other
grains, quinoa is not limiting in lysine and contains a bal-
anced ratio of amino acids and about 14 g protein, 7 g fiber,
and significant mineral content per 3½‐ounce (100 g) serving
(uncooked).35
Quinoa is gluten‐free and thus appropriate for those with
celiac disease. Its one drawback is the seeds’ bitter coating of
saponins, which can affect flavor and digestibility. Most com-
mercial quinoa is pre‐treated to reduce the saponin content,
but it is a good idea to additionally soak and rinse quinoa
before cooking. Quinoa is off‐white when uncooked and deep
tan or brown when cooked, and it has a fluffy, chewy texture
and nutty flavor. The grain is also available in red and black
varieties. Most simply, it can be prepared by simmering for
15 to 20 minutes at a 2:1 ratio of boiling water to quinoa. It
adapts well to pilafs and other seasoned dishes, even sweet
and breakfast porridges. It can also be processed to make
flour and flakes or sprouted for raw food diets.
Spelt is a wheat relative originating in Europe that con-
tains gluten and must be labeled in accordance with the
FIGURE 7.7 Market forms of grains.
Whole grains These are the least processed form of grains, containing bran, germ, and endosperm.
Whole grains are often referred to as groats. Examples include brown rice and wheat
berries. Because they contain the unsaturated oil‐rich germ, they must be refrigerated to
inhibit rancidity.
Pearled or polished The bran is partially or fully removed from the grain. Barley is referred to as pearled, and
rice is called polished.
Steel cut, cracked, or grits Whole or polished grains, such as oats, corn, or wheat, are cut into smaller pieces for
faster cooking.
Flakes or rolled Grains are steamed, then flaked or flattened between rollers. The process of flattening
the grain increases its surface area. The oils in the germ, as a result, could turn rancid. This
is avoided by steaming the grain, which inactivates the fat‐digesting enzymes.
Meal Grain is ground to a sandy consistency. Stone‐ground grains are ground between stones,
creating a grittier consistency. Examples include cornmeal and semolina.
Bran Bran is the thin layer just under the outer husk of the grain, which surrounds the endo-
sperm and is a rich source of fiber.
Germ The embryo of the grain is rich in fat, B vitamins, and vitamin E. It is made into a coarse
meal that must be refrigerated as the fat is susceptible to rancidity. Wheat germ is the
most common.
Flour This is the most prevalent market form of grain. The grain is ground into very fine powder,
either from the whole grain or just the endosperm. Any grain may be processed into this
form, as can nuts.
Starch The pure, finely ground carbohydrate portion of the grain with all protein, fiber, and fat
removed. Most commonly available: potato, corn, and rice.
Source: Hinnerk von Bargen.
c07.indd 123 1/14/2016 7:22:00 AM
124 • CULINOLOGY®
FALCPA regulations on wheat products. It is elongated and
wheat‐like in appearance. Spelt is commonly processed into
flour for breads and pastas rather than cooked in whole‐grain
form. Sprouted spelt breads are often found in health food
stores.
Amaranth is gluten free, and the plants may be used as
leafy vegetables, herbs, or grains depending on the species.
Amaranth’s edible seed is prized in cultures around the world,
including Central and South America and even Asia, but
most notably by the Incas and Aztecs. Amaranth grains are
small and round and contain a considerable amount of pro-
tein and relatively high lysine content. They are cooked like
quinoa, often with additional liquid (3:1 ratio) and for slightly
longer cook times, 20 to 25 minutes. Puffed versions of ama-
ranth may be cooked like couscous—that is, added to boiled
water, covered, and removed from heat to steam cook.
Toasted amaranth grains are mixed with honey, chocolate, or
molasses in traditional Spanish dishes. Amaranth is also
ground into flour and used in flatbreads.
Kamut® is the trademarked name (by Kamut International
Ltd. in 1990) for Khorasan wheat, a variety of ancient giant
wheat that contains gluten and is touted for its selenium con-
tent. The origins of Kamut® are in Egypt and the Middle East;
however, the grain has been cultivated and developed to its
present state in the United States. Kamut® may be cracked to
create a bulgur‐like product that is boiled and simmered, and
it is also processed into flour, breads, pastas, pastries, and
other foods. Any wheat sold using the name Kamut® must be
certified organic, have a protein content of 12 to 18 percent,
be 99 percent free of contaminating varieties of modern
wheat, contain between 400 and 1000 ppb of selenium, and
not be mixed with modern wheat in pasta. Even though this
wheat variety contains gluten, it is more easily digestible than
regular wheat by people with slight allergic tendencies.
Teff is the gluten‐free seed of a grass plant of African ori-
gin. It is available in chocolate brown and white varieties, and
is smaller than quinoa. Teff is available in its whole seed form
and is also ground into flour. Some people find it has a mild
acidic or sour flavor; others experience a molasses‐type fla-
vor. Teff has a mucilaginous texture and can become slimy if
cooked too long. It is most commonly cooked into ethnic
dishes such as Ethiopian injera, a fermented sponge‐like pan-
cake. Whole‐grain teff may be cooked at varying ratios (1:3 or
1:4 teff to water) and simmered for 10 to 20 minutes, depend-
ing on desired texture. Teff flour can be substituted for a por-
tion of wheat flour in baked goods. It is a good thickener for
vegan stews, soups, pudding, and gravy.
Millet is the tiny, gluten‐free seed of a grass plant thought
to have originated in Asia but with culinary traditions
throughout the world. In the United States, however, it is pri-
marily used as birdseed. Variations of Indian roti bread, por-
ridge (sweet and savory), and some alcoholic beverages use
this grain. Millet is often dry toasted to release its aroma,
covered in boiling water and simmered 20 minutes, and then
steamed for at least 5 minutes after cooking. It may also be
ground into flour and used for flatbreads, or sprouted. Millet
is comparable to wheat in protein and rich in vitamins and
minerals, but it may have adverse effects on the thyroid (due
to the presence of a peroxidase inhibitor) if consumed in
large amounts.
Although commonly mistaken for a grain, couscous is not
a grain at all but a tiny, flattened spherical pasta typically
made from wheat (semolina) flour. Common in North Africa
and the Middle East, couscous is available in the Western
market as instant couscous. This type cooks quickly: It is
added to boiling water, removed from heat, and covered to
steam for about 5 minutes. Couscous may also be steam
cooked over flavored simmering stews (Moroccan couscous
and tagines) in more traditional methods of preparation.
Whole wheat couscous and flavored couscous mixes are now
also available on the market. “Israeli couscous” is a larger
spherical pasta, roughly the size of tapioca pearls. Both sizes
go well with stews and tagine‐type dishes.
Seitan
Seitan, or “wheat meat,” is a vegetarian protein made from
de‐starched wheat dough. Seitan is traditionally used as a
meat replacer in Asian Buddhist vegetarian cuisines, simu-
lating the texture of duck or chicken. It consists entirely of
gluten, the allergenic component in wheat, and products
containing seitan must be clearly labeled with “wheat” on the
ingredient label, according to FALCPA. In addition, seitan is
not tolerated by anyone with celiac disease (gluten intoler-
ance). It has a spongy texture and an off‐white or gray to tan
color and can approximate meat products including cutlets,
nuggets, and chunks of chicken, duck, turkey, or beef. Seitan
has a relatively bland flavor on its own; however, it is typically
soaked in soy sauce or other marinade and is typically sold in
Asian groceries in jars along with this flavoring sauce.
How Seitan Is Made
In its simplest form, seitan is made by combining wheat flour
(bread or all‐purpose flour; cake flour is too low in protein)
and water, kneading intensely, rinsing away the starch with
water, and then cooking the finished dough in simmering
broth or other flavored liquid. Spices and other dry season-
ings may be added to the flour prior to mixing with water,
and stock or soy sauce may replace a portion of the water.
One method is to combine 1 part white flour, 1 part whole
wheat flour, and 1 part water to form a sticky dough that is
then kneaded several hundred times, submerged in water at
least 30 minutes or overnight, and then rinsed under cold
water for at least 30 minutes to remove all of the wheat
starch.36 Seitan dough can also be made with vital wheat glu-
ten (essentially pure gluten) in place of flour, eliminating the
rinse step.
The dough is then cut into pieces (cutlets, nuggets, fillets,
balls) and simmered in stock or other flavored liquid for
around 1 hour. The seitan puffs up, increases in size, and
develops a rubbery texture. While this simmering method is
most common in the West, Asian cultures may also steam,
boil, or fry the raw seitan dough. It is important to remember
that seitan dough greatly increases in size as it is heated, so it
c07.indd 124 1/14/2016 7:22:00 AM
CHAPTER 7 • Protein‐Based Foods: Vegetable Sources of Protein and Protein Complementation • 125
must be cut into smaller sizes than the desired finished
product. Commercially available brands are limited and vary
regionally.
Seitan can be used in sauces, stews, and soups, and it can
be stir fried or grilled. It requires minimal cooking—just
enough to absorb sauces and lightly grill or brown. Seitan can
be breaded and fried as a vegetarian cutlet; one useful appli-
cation is to gently simmer in wine, mushroom, or rich vegeta-
ble stock, wring out excess liquid, follow standard breading
procedure (flour, egg, bread crumbs), and pan fry as one
would chicken cutlets. Seitan can make a somewhat convinc-
ing vegetarian version of Thanksgiving turkey if served with
stuffing, cranberries, and vegetarian gravy. Seitan can also
approximate duck and makes excellent satay or kebabs when
marinated in a thick sauce and grilled.
Nutritional Profile
Seitan is very low in fat, quite low in carbohydrates, and high
in protein (some varieties contain up to 20 g per 3½‐ounce
[100 g] serving). Commercial seitan may be high in sodium
(up to 25 percent of daily allowance) when packed in soy‐
based marinade. When viewed as part of a complete vegetar-
ian diet, it is important to note that seitan is a grain protein
derived from wheat and therefore is limiting in the amino
acid lysine. Combining it with beans, corn, or nuts completes
the protein to provide all essential amino acids.
Nuts and Seeds
Once viewed primarily as snacks, nuts and seeds are gaining
popularity for their functional ingredients and ability to
boost the healthfulness of meals, particularly for vegetari-
ans. A significant amount of research and marketing have
gone into educating the public about the vitamins, healthy
fats, and trace micronutrients found in seeds and nuts. Non‐
peanut nut butters are gaining popularity as well. Note that
both tree nuts and peanuts (technically a legume) are
included in the FDA’s major eight allergens under FALCPA
and must be clearly labeled. Peanuts must be labeled as
such, while tree nuts (almonds, Brazil nuts, macadamias,
hazelnuts/filberts, cashews, walnuts, pecans, pistachios,
pine nuts, and even coconut) must be labeled as the specific
type of nut. While not legally included as allergens in the
United States, some seeds are viewed as allergens in other
countries, such as sesame seeds in Canada and celery seed
in the EU.
As the name indicates, tree nuts are harvested from large
plants or trees; they are often dried and either sold in the shell
(for certain varieties) or further processed to remove the
shell. Peanuts (also known as groundnuts) are harvested from
the soil beneath the plant (like potatoes) and cooked (roasted
or boiled) and dried. They may be sold in‐shell or shelled.
Both peanuts and tree nuts may be sold diced, ground into
flour, sliced, dry roasted, oil roasted, salted, ground into but-
ter, or as oil, among other forms.
Seeds are harvested from various plants and dried and
either sold in‐shell or shelled, and may also be roasted or
salted and flavored. The most common nutritional seeds (to
distinguish them from spice seeds) include sunflower, pump-
kin, pine nuts (pignolias), flax, sesame (white and black),
poppy, nigella, and hemp.
Storage and Shelf Life
Nuts and seeds are naturally very high in unsaturated oils
and tend to go rancid relatively quickly if stored at room
temperature. They also absorb odors readily. Other potential
problems are their attractiveness to rodents and tendency to
harbor larval insects that may grow at room temperature
conditions. Therefore, nuts and seeds should be stored in
sealed airtight containers under refrigeration to extend shelf
life. This is especially true of flax and hemp seeds, which
have among the most delicate oils when exposed to even
moderate heat. Nuts and seeds have a shelf life of up to one
year from date of production if properly stored; however, this
is reduced significantly when the nut is diced or roasted, as
its oils are more exposed and oxidize more quickly.
Nuts have received unfortunate media attention for their
ability to harbor dangerous pathogens, as evidenced in
recent salmonella outbreaks in peanuts and peanut butter,
pistachios, and pine nuts in 2009 and 2010. Therefore, many
nut processors are further increasing HACCP for nut han-
dling and processing, including reducing storage tempera-
tures and implementing additional control measures. After
contamination incidents in the past several years, almonds
must now be pasteurized for sale in the United States.
The food processor should note that tree nuts and seeds
are also commercially available in sterilized or pasteurized
forms. Nuts are typically sterilized using steam or propylene
oxide (PPO), while seeds may be treated with steam, ethyl-
ene oxide (ETO), or heat. These treatments lower the micro-
bial counts and the potential for growth of pathogenic
microorganisms. Roasting raw nuts and seeds (ideally
through a validated kill step) before consumption can also
lower microbial plate counts to enhance the safety of the fin-
ished product.
Preparation and Cooking Methods
Most commercially available nuts and seeds (other than
chestnuts) can be eaten as they are sold. For maximum fresh-
ness, purchasing whole shelled nuts and dicing or grinding
them just prior to adding to a dish is best. Many nuts and
seeds have improved flavor when they are gently roasted first.
Roasting temperatures and times vary on the type and cut of
the nut. Nuts and seeds are roasted in the oven or in a heavy
skillet over moderately high heat until they begin to turn
golden and release aroma. They can also be candied using a
coating of beaten egg whites and sugar, cinnamon, or other
sweet or savory spices.
Nuts are quite important in vegan and raw food diets
and may be soaked to change texture and to allow for grinding
into raw‐nut ricotta or cream sauce. Pine nuts are a classic
addition to pesto when ground with basil and garlic. Other
nuts are used in baking and pâtés, enrobed in chocolate,
c07.indd 125 1/14/2016 7:22:00 AM
126 • CULINOLOGY®
FIGURE 7.8 Protein in raw nuts and seeds
(shelled).
Nut/Seed (¼ cup) Protein (grams)
Almond 7
Brazil nut 5
Cashew 4
Chestnut 1
Coconut (shredded) 2
Filbert/hazelnut 5
Flax seed 5
Macadamia 2
Peanut 8
Pecan 2
Pine nut 4
Pistachio 6
Pumpkin seed 7
Sesame seed 7
Soynut 10
Sunflower seed 8
Walnut 5
Source: USDA National Nutrient Database for Standard Reference,
http://www.nal.usda.gov/fnic/foodcomp/search/.
made into candies, or added to savory pastas, stews, soups,
meats, and stuffing. Nut‐crusted fish or chicken fillets, coated
and fried, are popular menu items. Seeds may be toasted
or added directly to hot recipes—except flax and hemp
seeds, due to the volatility of their oils. They must be used
raw only.
Note: Because of the high level of attention that nuts in
particular receive as allergens, many food manufacturers
have eliminated them entirely from their facilities, primarily
driven by a fear of lawsuits.
Nutritional Profile
Nuts and seeds are high in protein and fat and are touted for
their contribution to a heart‐healthy diet. Flaxseeds, walnuts,
and pumpkin seeds are notably good vegetarian sources of
omega‐3 fatty acids such as alpha linoleic acid (ALA). When
combined with grains, they can yield a complete protein.
Trace micronutrients, such as selenium, are found in Brazil
nuts and other nuts.
Quorn®
Quorn is a fungal protein (mycoprotein) product developed
as an alternative to meat in the 1970s in the United Kingdom
as a response to global hunger. Quorn was created and trade-
marked by Marlow Foods and has been available in the U.K.
market since 1985 and in the United States since 2002.
According to SPINS, the information and service provider for
the Natural Products Industry, for the 52‐week period ending
May 17, 2009, Quorn products were the best‐selling frozen
meat‐free brand in natural food stores in the United States.
Quorn is low in calories and fat and high in dietary fiber; it is
considered a complete protein, with all the essential amino
acids present. Mycoprotein has a PDCAAS score of 0.91 ( just
behind beef at 0.92), and Quorn analogs also contain egg
white, which has a perfect score of 1.0.
Scientists researching ways to convert starch into protein
through controlled fermentation discovered a fungus called
Fusarium venenatum that can be used to ferment cereal
starch into single‐cell protein (SCP). The resulting mycopro-
tein is extracted, heat‐treated, dried, and mixed with egg
albumen as a binder. (Because of this, Quorn is vegetarian
but not vegan.) It is then textured, formed, and flavored in
products that mimic ground beef, chicken nuggets and fillets,
burgers, and meatballs. Currently, Quorn is available at retail
markets only, but Marlow’s patent ran out in 2010, and the
possibility exists that a company will start to manufacture
mycoprotein ingredients for industrial use in the near future.
Conclusion
The availability and range of vegetable‐based proteins is
enormous, and when combined strategically, vegetables can
be the sole source of protein in the diet. As an added benefit,
they can provide a host of other essential nutrients such as
vitamins, minerals, and fiber—and all this without the fat
and calories that normally accompany a diet heavily depend-
ent on meat. As consumers become more cognizant of these
benefits, the demand for new high‐quality vegetable‐based
retail and foodservice foods will continue to grow, and
Culinology® professionals will need both culinary creativity
and nutritional knowledge in order to take advantage of this
expanding market.
1U.S. Department of Agriculture, USDA National Nutrient Base for Standard
Reference, data file, retrieved from http://www.nal.usda.gov/fnic
/ foodcomp/search/, 2008.
2G. Sarwar and F. E. McDonough, “Evaluation of Protein Digestibility‐
Corrected Amino Acid Score Method for Assessing Protein Quality of
Foods,” Journal of the Association of Official Analytical Chemists 73 (1990):
347–356. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/2198245.
3K. D. Setchell, “Phytoestrogens: The Biochemistry, Physiology, and
Implications for Human Health of Soy Isoflavones,” American Journal of
Clinical Nutrition 68, Supplement (1998): 1333S–1346S. Retrieved from
http://www.joplink.net/prev/200101/ref/9‐43.html.
4M. S. Kurzer, “Hormonal Effects of Soy in Premenopausal Women and Men,”
Journal of Nutrition 132, no. 3 (2002): 570S–573S. Retrieved from http://
jn.nutrition.org/content/132/3/570S.full.
5J. M. Hamilton‐Reeves, G. Vazquez, S. J. Duval, W. R. Phipps, M. S. Kurzer, and
M. J. Messina, “Clinical Studies Show No Effects of Soy Protein or
Isoflavones on Reproductive Hormones in Men: Results of a Meta‐Analysis,”
Fertility and Sterility 94, no. 3 (2009): 997–1007.
6J. P. Harwood, L. M. Ausman, N. W. King, P. K. Sehgal, R. J. Nicolosi, I. E. Liener,
D. Donatucci, and J. Tarcza, “Effect of Long‐Term Feeding of Soy‐Based
Diets on the Pancreas of Cebus Monkeys,” Advances in Experimental
Medicine and Biology 199 (1986): 223–237.
c07.indd 126 1/14/2016 7:22:00 AM
http://www.nal.usda.gov/fnic/ foodcomp/search/, 2008
http://www.nal.usda.gov/fnic/foodcomp/search
http://www.ncbi.nlm.nih.gov/pubmed/2198245
http://www.joplink.net/prev/200101/ref/9%E2%80%9043.html
http://jn.nutrition.org/content/132/3/570S.full
http://jn.nutrition.org/content/132/3/570S.full
CHAPTER 7 • Protein‐Based Foods: Vegetable Sources of Protein and Protein Complementation • 127
7Edensoy, product information, retrieved from http://www.edenfoods.com, 2009.
88th Continent, product information, retrieved from http://www.8thcontinent
.com, 2009.
9Edensoy.
10Vitasoy, product information, retrieved from http://www.vitasoy‐usa.com,
2009.
11Silk, product information, retrieved from http://www.silksoymilk.com, 2009.
12USDA, National Nutrient Base.
13American Heritage Dictionary, 4th ed. (New York: Houghton Mifflin, 2001).
14Nasoya, product information, retrieved from http://www.nasoya.com, 2009.
15H. L. Wang and C. W. Hesseltine, “Coagulation Conditions in Tofu
Processing,” Process Biochemistry ( January/February 1982): 7.
16W. Shurtleff and A. Aoyagi, Tofu and Soymilk Production: A Craft and
Technical Manual, 3rd ed. (Lafayette, CA: Soyfoods Center, 2000), retrieved
from http://www.soyinfocenter.com/.
17W. Shurtleff and A. Aoyagi, A. (2008). A Comprehensive History of Soy:
History of Soybeans and Soyfoods, Past, Present, and Future (Lafayette, CA:
Soyinfo Center, 2008), retrieved from http://www.soyinfocenter.com/.
18Ibid.
19Ibid.
20USDA, National Nutrient Base.
21Nasoya.
22S. Areekul, S. Pattanamatum, C. Cheeramakara, K. Churdchue, S.
Nitayapabskoon, and M. Chongsanguan, “The Source and Content of Vita-
min B12 in the Tempehs,” Journal of the Medical Association of Thailand 73,
no. 3 (1990): 152–156.
23Food Service Direct, product information, retrieved from http://www
.foodservicedirect.com, 2009.
24Turtle Island Tempeh, product information, retrieved from http://www
.tofurky.com/faq_tempeh.html, 2009.
25W. Shurtleff and A. Aoyagi, The Book of Tempeh, prof. ed. (New York: Harper
and Row, 1979).
26Archer Daniels Midland, Textured Vegetable Protein, retrieved from http://
www.adm.com, 2009.
27USA Emergency Supply, product information, retrieved from https://www
.usaemergencysupply.com, 2009.
28Archer Daniels Midland, Textured Vegetable Protein.
29USDA, National Nutrient Base.
30U.S. Department of Health and Human Services and the U.S. Department of
Agriculture, 2010.
31B. D. Smith, The Emergence of Agriculture, Scientific American Library (New
York: W. H. Freeman, 1998).
32N. M. Edwards, S. J. Mulvaney, M. G. Scanlon, and J. E. Dexter, “Role of
Gluten and Its Components in Determining Durum Semolina Dough
Viscoelastic Properties,” Cereal Chemistry 80, no. 6 (2003): 755–763.
doi:10.1094/CCHEM.2003.80.6.755. Retrieved from http://cat.inist.fr/?aMo
dele=afficheN&cpsidt=15273405.
33U.S. Food and Drug Administration, “Foods Labeled Gluten‐Free Must Now
Meet FDA’s Definition,” data file, retrieved from http://www.fda.gov/Food
/NewsEvents/ConstituentUpdates/ucm407867.htm, 2014.
34A. Nilsson, et al., “Effects on GI and Content of Indigestible Carbohydrates
of Cereal‐Based Evening Meals on Glucose Tolerance at a Subsequent
Standardised Breakfast,” European Journal of Clinical Nutrition 60, no. 9
(2006): 1092–1099.
35USDA, National Nutrient Base.
36Natural Gourmet Cookery School, Seitan recipe, Chef ’s Training Program
Course, 2004.
c07.indd 127 1/14/2016 7:22:00 AM
http://www.8thcontinent.com, 2009
http://www.foodservicedirect.com
http://www.tofurky.com/faq_tempeh.html
http://cat.inist.fr/?aModele=afficheN&cpsidt=15273405
http://www.fda.gov/Food/NewsEvents/ConstituentUpdates/ucm407867.htm
http://www.edenfoods.com
http://www.vitasoy%E2%80%90usa.com
http://www.silksoymilk.com
http://www.nasoya.com
http://www.soyinfocenter.com
http://www.soyinfocenter.com
http://www.adm.com
http://www.adm.com
https://www
128 • CULINOLOGY®
Lead Author: Allen J. Freed , President, AJ Freed, L.L.C.
Technical Manager , Grain Processing Corporation
Contributor: Hinnerk von Bargen , CHE, Associate Professor in Culinary Arts , Culinary Institute
of America
Contributor: Jason R. Gronlund , VP Culinary , Smokey Bones Bar & Fire Grill, Johnson & Wales
Alumnus
8
Carbohydrate‐Based
Foods
What Are
Carbohydrates?
Carbohydrates are designed to be the primary source of
energy in the body. The word carbohydrate is made up of two
components that very clearly (at least on a chemical basis)
tell you about its structure. The carbo portion is derived from
the word carbon , and the hydrate portion comes from the
term for water (H
2
O), a molecule composed of two atoms of
hydrogen and one oxygen atom. Therefore, a carbohydrate is
a molecule that contains both carbon and water. A simple
carbohydrate, such as glucose , has the chemical formula
C
6
H
12
O
6
. For every 6 carbons there are 12 hydrogen atoms and
6 oxygen atoms, but the ratio of hydrogen to oxygen is still 2:1,
like water.
Foods we associate with carbohydrates are sugars, flours,
starches, grains, cereals, potatoes, rice, and foods made with
these ingredients: pasta, bread, French fries, rice pilaf, and so
on. In fact, the vast majority of carbohydrates come from
plant sources: fruits and vegetables, grains, and legumes.
These plants are the source materials from which carbohy-
drates, in the form of sugars, starches, gums, and fiber, are
extracted, purified, concentrated, and then used as ingredients
to create or enhance other foods. Some of the ingredients are
obvious, such as cornstarch and high‐fructose corn syrup
(HFCS), derived from a portion of the corn kernel. Other
ingredients, such as gum acacia, which comes from the sap of
the acacia tree, are less so.
Carbohydrates
as an Ingredient:
The Chef ’ s View
From a culinary standpoint, unlike the other two energy‐
containing macronutrients, which are primarily eaten directly
as foods themselves ( proteins ) or indirectly consumed as the
result of cooking (other than butter on bread or oil in salad
dressings, we generally don ’ t eat pure fats and oils directly),
carbohydrates fit into both camps: the starchy vegetables,
grains, and legumes that are eaten as is, and the functional
ingredients, such as starches, flours, and meals, that can be
extracted from them (potato starch, rye flour, wheat flour,
and cornmeal are just a few examples). In addition, carbohy-
drates make up the vast majority of foods consumed around
the world.
Grains, Legumes, and
Starchy Vegetables
Starchy vegetables, grains, and legumes are often regarded as
plate fillers, unglamorous foods that accompany the more
flamboyant main dish. Prepared correctly, however, they can
be extremely delicious, sometimes with the result that they
become the star on the plate.
Dan Putnam ,
H
O
H
H
H
H
OH
OH
OH
CH2OH
OH
FIGURE 8.1 Simple sugar molecule.
Source: Gum Technology Corporation.
c08.indd 128 1/22/2016 1:07:05 PM
CHAPTER 8 • Carbohydrate‐Based Foods • 129
Grains
The seeds of grass plants, known as grains, with their high
nutritional value, long shelf life, and comparatively low cost
of cultivation, are arguably the most important food crops for
the human diet. According to Jared Diamond in his book
Guns, Germs, and Steel, grains were domesticated as early as
9000 bc. Today, the three largest grain crops grown world-
wide are corn, wheat, and rice.
Realizing that many other animals relied on the seeds of
grass plants for sustenance, people considered them a possi-
ble alternative to the berries, nuts, roots, and other gathered
and hunted foods of the forest. However, early humans had to
figure how to locate, identify, cook, and, eventually, grow
these grains. They had to determine which ones were the
most nutritious and how they were best prepared. At first
there was no one to teach them that grains had to be husked,
ground more or less finely, and then cooked or baked with the
addition of some liquid. All of the unique preparations spe-
cific to each grain were discovered over time by trial and
error, and mostly by chance.
For example, it is likely that a fermented batch of rye
dough somewhere in northern Europe several thousand
years ago gave birth to the idea of sourdough. Whole‐grain
breads taste best when the dough is properly fermented,
either with yeast or a bit of previously made sourdough.
Nutritional science has revealed that phytates in the bran of
wheat or rye hinder zinc and iron absorption. Proper fermen-
tation of the dough destroys phytates, making the bread
more nutritious.
Hominy is another example of mankind’s resourcefulness
when it comes to getting the best out of a grain. In a tradi-
tional Native American process called nixtamalization, corn
is soaked in alkali (either sodium hydroxide or potassium
hydroxide). During this process, niacin and other B vitamins
are converted into a more absorbable form, and the availabil-
ity of amino acids is improved. When corn was introduced to
Spain and Italy in the early 1500s without the knowledge of
this traditional treatment, poor sharecroppers, who during
the cold winter months relied exclusively on corn for suste-
nance, suffered the four Ds: diarrhea, dermatitis, dementia,
and death by the end of winter. These are symptoms of pel-
lagra, a niacin deficiency.
Today, along with the staple crops of corn, wheat, and rice,
there are dozens of other grains, both modern and ancient
(“what’s old is new again”) such as barley, oats, quinoa,
freekeh, and buckwheat that are available to the culinary pro-
fessional in a wide variety of market forms.
Legumes
Second only to grains in importance to human nutrition are
legumes, the seeds of leguminous plants such as beans, peas,
and lentils. All legumes grow as seeds in pods. Rich in com-
plex carbohydrates, fiber, proteins, and minerals, they play an
important role in many diets all over the world. In order to
expedite their cooking, dried legumes are first soaked until
hydrated and then cooked in liquid until very tender. For
lentils, black‐eyed peas, pigeon peas, and split peas, soaking
is less critical; these legumes cook relatively quickly without
this step. For a more in‐depth look at the legumes, see the
write‐up in Chapter 7: Vegetable Sources of Protein.
Starchy Vegetables
Besides grains and legumes, starchy vegetables such as pota-
toes, plantains, and taro root (to name just a few) are an
important staple in many parts of the world. Relatively low in
moisture and with good shelf‐life properties, they share a
vital spot on the world’s plates.
Native to South and Central America, potatoes have made
their way into almost every culture. When first introduced to
the Old World by returning conquistadors, they were met
with great suspicion. In fact, at first they were used only as
animal feed. Potatoes are a member of the nightshade family,
and they have toxic leaves; if stored improperly, the spuds can
become toxic, too. When exposed to sunlight, solanine, a
toxic alkaloid, can develop on the skin and around the eyes of
the potato. It is commonly yet incorrectly believed that the
green color that develops on the exposed parts of the potato
is the toxin; actually, it is chlorophyll. However, the green
color serves as a convenient warning sign: wherever there is
chlorophyll, solanine is just below the skin. Both chlorophyll
and solanine are easily removed by peeling.
Potatoes are rich in vitamin C, which made them helpful in
preventing scurvy on long ocean voyages. Some nutritionists
have claimed that the enormous quantity of French fries con-
sumed in the United States prevents vitamin C deficiency here.
Growing well in moderate climates with mild summers
and cool winters, potatoes became the single most impor-
tant crop in Ireland. During the eighteenth and nineteenth
centuries, one‐third of the Irish population relied almost
exclusively on potatoes for sustenance. The great potato fam-
ine of the 1840s (due to a blight caused by a fungal infection)
brought about the death of as many as a million people due
to starvation and disease. This led to mass migration to the
New World. It is a curious fact of culinary history that pota-
toes, arriving in the United States via Ireland, were brought
here by the people who almost died because of them.
Potatoes are more adaptable to a wide range of cooking
methods than most other vegetables. Selecting the correct
type of potato for any given cooking method is the key to suc-
cess. Russet potatoes are a great choice for mashed potatoes
and French fries; the relatively high starch content makes the
purée fluffy and creamy and allows the fries to become crisp
on the outside while remaining creamy in the center. However,
they are less suitable for salads, boiled potatoes, and other
preparations where maintenance of the shape is desired. The
swelling of the starch granules during the gelatinization
process causes the fibers to break excessively, and the potato
falls apart during cooking. “Waxier” varieties with lower
starch content, such as Yukon Gold, Red Bliss, and yellow
potatoes, are better suited to these methods.
Another common problem in many kitchens and food‐
processing facilities is gooey mashed potatoes. When the
c08.indd 129 1/14/2016 7:22:28 AM
130 • CULINOLOGY®
potatoes are puréed properly with a food mill or a ricer
(which minimizes shear), less than 5 percent of the starch
cells are disrupted, resulting in fluffy and tender mashed
potatoes. Too much shear force caused by a food processor
or electric mixer (or even just excessive stirring) causes
many more starch cells to rupture. The released starch
absorbs all free water, creating mashed potatoes resem-
bling glue.
An often misunderstood potato category is new potatoes,
not to be confused with baby potatoes (which are simply
small potatoes). True new potatoes are available only at the
beginning of the new harvest, when the plant part above the
ground has not wilted yet. At this stage, the potatoes have
not converted all sugars into starch, and they maintain a
slightly sweet flavor. Their skin is very delicate and can be
removed by light brushing. Other types of potatoes include
fingerling potatoes and the colored (blue, purple, yellow, red)
potatoes.
Sweet potatoes are botanically unrelated to potatoes. The
pale, yellow type is dry and crumbly, and the darker, orange
variety is moister and sweeter. Culinary uses of the sweet
potato, however, are similar to those of potatoes. The orange
variety with the pointy ends is often referred to as yams. True
yams, however, are unrelated to sweet potatoes. Originating
in Africa, yams are slightly sweeter than sweet potatoes and
can grow up to 7 feet in length!
The plantain, looking much like a large banana, is actually
a starchy fruit and not a vegetable. Nevertheless, it cannot be
eaten raw. Depending on the desired dish, it is used either
ripe (maduro) or green (verde). Becoming very soft upon
cooking and tasting much like bananas, ripe plantains are
used in casseroles and purées or fried and served as a sweet
side dish. Green plantains, on the other hand, are mostly used
as a potato substitute in soups and stews, and are used in
many parts of the Caribbean and Latin America to make
tostones—thick slices of crispy fried plantain.
Other starchy vegetables, including taro root, cassava,
arrowroot, and jícama, are widely cultivated in many cultures.
Flours
All grains, legumes, and starchy vegetables can be dried and
ground into fine flours or more coarsely into meal. All grain‐
based flours (wheat, rice, rye, barley, corn, and so on) are
called whole‐grain flour when the grains are husked and then
ground into a fine powder. For more refined flours, only the
endosperm, containing the starch and protein, is ground.
For flours based on legumes such as soybeans, lentils, and
chickpeas (known as besan in Indian cuisine), the outer husk
or membrane skin is removed, and the dry legume is then
ground to a fine powder. For the less common flours based on
starchy vegetables, like potato flour, the item must be fully
dehydrated before it is ground.
A large variety of flours is available to culinary profession-
als. With distinct flavors and thickening and binding proper-
ties, a wide assortment of flours are used in ethnic cuisines.
Refined Starches
Pure starches are produced by separating the starch from the
surrounding insoluble cellulosic materials in the plant. Potato
starch, for example, can be obtained by grating a raw potato
with a fine grater into a cheesecloth‐lined bowl filled with cold
water. Squeezing the grated potato solids in the cheesecloth
presses out the majority of the water along with the potato
starch. After allowing the starch to settle, the water can be
decanted; the starch remains at the bottom of the bowl.
Any starchy vegetable is a potential source for starch;
the more common starches—cornstarch, potato starch,
arrowroot—are available in any grocery store. They are most
frequently used for thickening liquids and as a binding agent
for fillings.
FIGURE 8.2 Starch and moisture content of
potatoes, and corresponding cooking methods.
Types
Moisture
Content
Starch
Content
Cooking
Method
Idaho or
russet
Low High (mealy,
low sugar)
Bake,
purée, fry
Chef’s,
all‐purpose,
Yukon Gold
Moderate Moderate Boil, sauté,
steam, roast,
braise, stew
New
potatoes
High Low (waxy,
high sugar)
Boil, steam,
roast, stew
Red Bliss Moderate Low Boil, steam
Sweet
potatoes,
“yams”
Moderate
to high
High Boil, bake,
purée, roast
FIGURE 8.3 Basic makeup of a grain.
Bran
Germ
Endosperm
c08.indd 130 1/14/2016 7:22:28 AM
CHAPTER 8 • Carbohydrate‐Based Foods • 131
Each starch lends itself to specific applications. From a chef ’s
perspective, starches can be divided into two major groups:
cereal starches, such as corn and rice starch, and root starches,
such as arrowroot and potato starch. The makeup of the starches
lends distinct properties to each group. Root starches com-
monly have a higher content of amylopectin, whereas cereal
starches are generally richer in amylose. As a result, liquids thick-
ened with cereal starches end up a little cloudy but relatively
resistant to excessive boiling, long hot holding, and mechanical
shear. In addition, they retrograde; upon cooling, these liquids
form a sliceable gel, making them suitable for puddings and pie
fillings. The drawback is that retrogradation eventually results in
syneresis and, consequently, moisture loss.
In comparison, the use of root starches results in crystal
clear and smooth liquids that are unfortunately unstable to
excessive boiling, mechanical shear, and long hot holding.
Root starches do not retrograde, making them less suitable
for fillings, unless it is desired that the filling ooze out like a
ripe camembert cheese. Root starches are suitable if clarity
and a very smooth mouthfeel are desired.
Starches alone are often used to thicken sauces; the clar-
ity and smoothness of a jus lié (“thickened juice”) makes this
the perfect accompaniment for a standing rib roast. If how-
ever, a pan gravy is desired, a roux should be used. Pan gravy
is thicker than a jus lié and is expected to be velvety rather
than glaze‐like. A sauce thickened with starch to the same
viscosity as a roux‐thickened sauce has a slightly mucoidal
mouthfeel. In addition, a roux‐thickened sauce tends to
be more stable to shear, long heating, and freezing and
thawing.
Important in Far Eastern cooking, the more exotic starches
such as tapioca, rice, mung bean, and sweet potato are mainly
available in ethnic grocery stores. The exotic noodles, such as
sweet potato noodles in Korea and mung bean starch threads
in China and Vietnam, are evidence of the creative use of
starches beyond thickening and binding.
For most starch‐based noodles, a starch slurry is forced
through a perforated disk into boiling water, resulting in long
strands. Depending on the starch, these noodles can be crys-
tal clear, like those based on sweet potato starch, or opaque,
like rice noodles. For other varieties of starch‐based noodles,
the starch slurry is steamed into sheets that are cut into rib-
bon‐style noodles. Thai rice noodles and Chinese mung bean
noodles are good examples.
Another ingenious way to use starch is tapioca pearls.
Tapioca is not used as much for thickening; a liquid thick-
ened with pure tapioca starch is very clear but unpleasantly
mucilaginous. Instead, the starch is processed into pearls or
other shapes for textured beverages such as bubble tea or as
a textural thickening component for tapioca pudding or fresh
fruit pies.
Depending on geography, climate, and many other fac-
tors, the starches, flours, and starchy vegetables used in a
cuisine vary tremendously. People had to come up with
ingenious ideas to make food digestible and get the nutri-
tional and epicurean bang for the buck. This ingenuity
reflects the universal need to prepare a meal not only for sus-
tenance but also for pleasure. At times, it seems we invest an
unreasonable amount of energy in the preparation of food;
it’s much easier to just eat whole‐grain porridge. However,
thinking about all the good times spent at dining tables
makes one realize that a good meal is much more than the
sum of its nutrients.
FIGURE 8.4 Non‐wheat flours, their origin
and use.
Flour Origin Uses
Amaranth flour South America,
South Asia
Puddings,
gluten‐free
baking, salads
Buckwheat flour Eastern Europe,
Japan
Pancakes, hot
cereals, baking,
noodles
Chestnut flour Mediterranean,
Italy
Polenta in Corsica,
baking, pasta
Chickpea flour
(besan)
India Batters, breads,
thickener
Corn flour/meal South Asia,
Americas
Hot cereal,
bread, thickener,
cornbread, grits,
polenta/mush
Dried potato
flour (chuño)
Western South
America
Thickener
Rice flour Southeast Asia Rice paper, sweets,
desserts
Rye flour Central Europe Baking
Tapioca flour Central Africa Baking, pancakes,
fufu
Teff Ethiopia Baking, pancakes
Pease meal
(ground yellow
peas)
Great Britain Porridges, soups,
baking
Masa harina
(ground dried
hominy)
Mexico Tamales, corn
tortillas/tacos
FIGURE 8.5 Comparison of root and cereal
starches.
Root Starch Cereal Starch
• Thickens quickest at lower
temperatures
• Clear
• Thickest when hot
• Breaks down from mech-
anical shear, prolonged
cooking, and hot holding
• Stays viscous during cooking
• Requires higher
temperature to thicken
• Less clear
• Retrogrades
• Syneresis
• Stable during mechanical
shear, prolonged
cooking, and hot holding
c08.indd 131 1/14/2016 7:22:28 AM
132 • CULINOLOGY®
When we take in more carbohydrates than we can use
right away, initially the excess glucose is stored in the liver
and muscles as glycogen, which is a form of rapid‐release
energy accessed by the body when it has used up its free glu-
cose and needs replenishment. Glycogen is stored in
extremely limited amounts, however, and once it is used up
the body must replace its energy stores by consuming addi-
tional nutrients or by breaking down fat (the ultimate storage
form of energy) and, ultimately, protein (but only as a last
resort, after fat stores are depleted).
If more glucose is available beyond that which can be
stored as glycogen, additional consumed nutrients (includ-
ing carbohydrates) are turned into fat. If too much fat is
stored, the body becomes overweight or obese. An obese
person is someone who, as defined by the World Health
Organization (WHO), the U.S. Centers for Disease Control
(CDC), and the U.S. Department of Health and Human
Services (DHHS), has a body mass index (BMI) greater than
30. The body mass index is calculated by dividing weight in
pounds by height in inches times your height in inches again,
and multiplying that by 703. The odd number of 703 is calcu-
lated by converting the original metric formula, also shown
below.
O
OH OH
CH2OH
Mannose Mannose Glucose Glucose
O
OH OH
CH2OH
O
O
OH
OH
O
OH
OH
CH2OH
O OO
CH2 O C CH2
O
FIGURE 8.7 Polysaccharide.
Source: Gum Technology Corporation.
Carbohydrates
in Nutrition
Carbohydrates, along with proteins and fats, constitute the
three primary biomolecules from which humans get
calories.
Sugars are the simplest carbohydrates. They fall primarily
into the monosaccharides (glucose, galactose, and fructose,
often called simple sugars) and disaccharides (sucrose, malt-
ose, and lactose), which are made from the monosaccharides.
Larger molecules called polysaccharides, made of many sim-
ple sugars bound together, form the other major class of car-
bohydrates: starches, glycogen, fiber, and gums, known as
complex carbohydrates. An intermediate group of carbohy-
drates known as the oligosaccharides (a good source of prebi-
otics) are especially important in the gut, where they promote
the growth of good bacteria (probiotics).
Carbohydrates are essential to good human nutrition.
Most nutritionists feel that people should get 50 to 60 percent
of their daily caloric intake from complex carbohydrates.1
One gram of carbohydrate contributes 4 kcal (calories) to our
diet. The body uses glucose, the ultimate breakdown product
of virtually all digestible carbohydrates, to create energy for
cellular respiration and to control brain functions. Glucose is
the sugar we find in our blood. The word is from the Greek
word glykys, meaning “sweet,” and the ‐ose ending, which
means “sugar.” That said, the common term in the food indus-
try for glucose is dextrose. In truth, glucose and dextrose are
virtually identical.
The body breaks down all carbohydrates into monosac-
charides. It then further breaks those down through a proc-
ess called glycolysis to produce adenosine triphosphate
(ATP). ATP is the molecule that actually provides energy for
all vital processes in our bodies.
H
H
H
H
OH
OH
OH
CH2OH
OHO
FIGURE 8.8 Glucose molecule.
Source: Gum Technology Corporation.
H
H
H
OH
HO
OH
CH2OH
OC
C
H
C
C
OH
H
C
FIGURE 8.9 Fructose molecule.
Source: Gum Technology Corporation.
FIGURE 8.6 Disaccharide.
Source: Gum Technology Corporation.
H
O
H
H
OH
HO
OH
CH2OH
H
O
H
H
OH
OH
CH2OH
H
OH
H
O
c08.indd 132 1/14/2016 7:22:29 AM
CHAPTER 8 • Carbohydrate‐Based Foods • 133
BMI
Weight in Pounds
Height in Inches Height in Inches
( )
( ) ( )
703
or
BMI
Weight in Kilograms
Height in Meters Height in Meters( ) ( )
Obesity can lead to a wide range of health problems,
including diabetes, heart disease, cancer, stroke, sleep apnea,
and osteoarthritis.2 In fact, the CDC’s annual statistical
review of causes of death considers overweight and obesity
an increased risk factor in 10 percent of all deaths in the
United States.
Glucose is essential to brain functions, and so the amount
in the blood is carefully regulated by the pancreas, which
releases insulin in response. Insulin delivers glucose to the
cells in your body and also helps change it into glycogen for
storage in the liver and muscles. Insulin also helps convert
excess glucose to fat.
Diabetes is a disease that occurs when the body cannot
process the glucose because the pancreas does not produce
enough (or any) insulin and the body can’t convert the glu-
cose into glycogen, or because the body does not handle the
insulin properly and not all of the glucose is converted into
glycogen. If untreated, diabetes can lead to heart disease and
kidney damage as well as increased chances of hypertension
and high cholesterol. In extreme cases, blindness, amputa-
tion of the extremities, diabetic coma, and death can result.
Diabetes, if not too severe, can be treated successfully by diet
and exercise, but in more serious cases, sufferers must take
insulin shots on a daily basis.
Carbohydrates
as Ingredients
Carbohydrates are more than just a source of fuel for the
body. In food manufacturing, they are useful in creating struc-
ture, texture, and mouthfeel in foods, and when used in this
context are referred to as functional food ingredients. Some of
these can help build viscosity and maintain suspension of
particulate matter, such as herbs in salad dressings, or help
suspend flavor inclusions, such as fruit bits in batters or
yogurt. Other carbohydrates can help create and maintain an
emulsion (for example, mayonnaise or creamy salad dress-
ing). Carbohydrates can also help retain moisture, reduce
crystallization in frozen foods, prevent staling in baked
goods, and keep food fresh longer by holding onto water and
preventing the food from dehydrating. Sugar, of course, can
also add sweetness.
Some carbohydrates are used to add bulk, replacing high‐
calorie ingredients such as fats with lower‐calorie ingredients
such as fiber. Fiber itself provides valuable nutritional benefits
that aid in digestion and nutrient assimilation. Studies show
that soluble fiber helps reduce blood cholesterol and coro-
nary disease. Insoluble fiber absorbs and holds onto water in
the intestine, helping waste pass more quickly through the
digestive tract, which may help prevent colon cancer. Fiber
also adds to a feeling of satiety by making the stomach feel
full, leading to reduced intake of foods.
The carbohydrates can be divided into four primary ingre-
dient groups: sugars, starches, gums, and dietary fiber.
Starches, gums, and fiber fall into the complex carbohydrate
category, while sugars (monosaccharides, disaccharides, oli-
gosaccharides) may be simple or complex. Gums and fiber
are not digestible by humans and therefore provide no calo-
ries in the diet, nor are they a source of energy. They do have,
however, an enormous influence on food formulation and
health.
Sugars
The FDA defines sugars as monosaccharides (glucose, fruc-
tose, and galactose) and disaccharides (lactose, maltose, and
sucrose) in Title 21 Code of Federal regulations, Section 101.9(c)
(6) (ii).3 Monosaccharides are the smallest molecular units of
the carbohydrates; they cannot be hydrolyzed (broken down)
to smaller carbohydrates. A molecule consisting of two mono-
saccharides is classified as a disaccharide.
Sugars are used in food systems for a variety of functional
reasons, with sweetness being the most obvious. Each sugar
has its own level of sweetness and is compared to that of
table sugar (sucrose).
Sugars are also used in formulations to affect humectancy
(the ability of a product to resist loss of moisture)—an excel-
lent example is soft, chewy cookies. A sugar is considered
hygroscopic (able to absorb moisture) when it has a high
degree of humectancy.
Some sugars are known as reducing sugars; these are used
to promote browning and add flavor to foods. Reducing
sugars include all of the monosaccharides and two of the
disaccharides (lactose and maltose, but not sucrose). All have
a highly reactive end on their molecular structure that works
in conjunction with the proteins present in a food (and some
form of dry heat) to form compounds that produce distinc-
tive brown flavors, colors, and aromas. This reaction is called
Maillard browning (one form of non‐enzymatic browning);
examples include the browned crust in baked or toasted
breads and cakes, roasted coffee beans, chocolate, and the
color, flavor, and recognizable smells of grilled or roasted
meats.
Caramelization is another form of non‐enzymatic brown-
ing, but it does not rely on the presence of proteins or amino
acids. Rather, the browning is the result of pyrolysis (chemi-
cal breakdown of the sugars due to heating), and any sugar,
including sucrose (most often used for this purpose) can
participate in the reaction. Typical examples are flan,
crème brûlée, and the browning of vegetables by grilling or
sautéing.
Many formulations take advantage of the relatively small
molecular size of sugars. Water activity (aw) is a measurement
used to determine the amount of unbound or free water in a
food system. This should not be confused with the food’s
moisture content, or a
w
; it reflects only the water in a food
system available for spoilage reactions or microbial metabolic
c08.indd 133 1/14/2016 7:22:29 AM
134 • CULINOLOGY®
processes to occur. The more molecules of solute are dis-
solved in an aqueous system (the solvent), the more water is
bound to them and the less is available to take part in delete-
rious reactions such as hydrolytic rancidity in fatty foods,
moisture migration in baked goods, and microbial spoilage.
Sugar molecules are relatively small compared to most other
ingredients (such as starches, fats, fiber, and proteins), and
thus, considering equal weights of each, more sugar mole-
cules are present than molecules of any other ingredients.
With more molecules dissolved and tying up more water
molecules, less free water is available, resulting in signifi-
cantly reduced water activity. In general, reducing water
activity prolongs the shelf life of a food. (Increasing the salt
concentration yields a similar benefit but is appropriate for
savory foods only.)
Another important function of sugars is the role they play
as a substrate for fermentation in foods such as yeast‐raised
baked goods, alcoholic beverages, fermented sausages (like
pepperoni), cultured dairy, and pickled vegetable products.
Monosaccharides
Glucose (Dextrose)
The terms glucose and dextrose are used interchangeably,
with certain food industry segments or regions preferring
one over the other. Glucose is the most common hexose
(6‐carbon sugar) found in foods, and it is naturally present in
fruits, honey, and some vegetables. It also serves as the build-
ing block for virtually all other carbohydrates, from disaccha-
rides to gums to the enormously large and complex starches
and fiber. Commercially, glucose is produced by the complete
hydrolysis of starch, and in the United States is made almost
exclusively from corn starch. Glucose is a hygroscopic reduc-
ing sugar with lower sweetness than table sugar. The most
readily fermentable carbohydrate, it is used extensively in
commercial yeast‐raised baked goods. Glucose is a compo-
nent in regular and high‐fructose corn syrups, providing each
a significant portion of their characteristic functionalities of
humectancy (moisture holding) and browning. Commercial
glucose is available as a fine powder, in crystalline form, and
as a high‐solids syrup.
Fructose (Levulose)
Fructose is the second most common monosaccharide. This
reducing sugar is produced commercially by enzymatically
converting glucose. In the United States, it is commercially
derived from cornstarch. Fructose is found in nature in many
fruits, which explains its other name: fruit sugar. It is the
sweetest of the natural sugars, considered 1.5 times sweeter
than table sugar. Fructose is unique in that it is found with
two isomers (identical formulas but different shapes) that
coexist in solution. One shape has a sweetness rating at or
below that of sucrose, the other much higher. Temperature
and acidity affect which shape predominates and are the rea-
son hot tea or coffee sweetened with fructose is less sweet
than that sweetened with an equal amount of table sugar.
Fructose is considered an excellent humectant and provides
moisture retention for extended periods—soft, chewy cookies
being a prime example. Commercially fructose is available as
a crystalline solid, but the use of pure fructose is limited due
to cost and difficulty of storage and handling because of its
high degree of hygroscopicity (ability to absorb moisture from
the air) and resultant stickiness. Instead, fructose is most
commonly used in combination with dextrose in high‐fruc-
tose corn syrup.
The glycemic index (GI) is a measure of the effect of car-
bohydrates on blood sugar levels. A carbohydrate absorbed
into the bloodstream quickly, such as glucose, is considered
to have a high GI, and one absorbed into the bloodstream
more slowly, such as oat bran, is considered to have a low GI.
Recent scientific evidence shows that individuals who follow
a low‐GI diet over many years are at significantly lower risk
than others for developing both Type II diabetes and coro-
nary heart disease. High blood glucose levels or repeated gly-
cemic spikes following a meal may promote these diseases by
increasing stress to the blood vessels and also by the direct
increase in insulin levels.4 Fructose is not digested the same
way as other sugars (it is exclusively metabolized in the liver),
and as a result has a low glycemic index. For example, glucose
has a GI of 100, because it is quickly digested and released
into the blood. Sucrose has a GI of 65, because it is 50 percent
glucose. Fructose, in comparison, has a GI of only 19, lowest
of all the natural sugars. That said, recent evidence shows
that too much fructose in the diet may result in the develop-
ment of insulin resistance, liver disease, and a condition
known as metabolic syndrome that includes obesity, elevated
triglycerides and decreased HDL (good cholesterol) in the
blood, and elevated blood pressure. It is always better to eat a
varied diet and spread the risks rather than to restrict the diet
to a limited number of potentially harmful foods.
Galactose
Galactose together with glucose makes up lactose, or milk
sugar. Galactose is about one‐third the sweetness of sucrose,
but it does supply energy (calories), so it is considered a nutri-
tive sweetener. Its primary function in processed foods is as a
component of hydrocolloid gums (galactomannans such as
gum Arabic, agar‐agar, and guar gum), commonly used for
moisture management in a variety of foods.
Disaccharides
Maltose (Malt Sugar)
Maltose is a disaccharide formed from two units of glucose.
Its chief importance in food processing is as a fermentable
substrate for yeast in the production of alcohol (ethanol), par-
ticularly in the brewing industry.
During malting, cereals, especially barley, are soaked and
then allowed to germinate in order for amylase enzymes in
the grain to become activated and to start hydrolyzing the
c08.indd 134 1/14/2016 7:22:29 AM
CHAPTER 8 • Carbohydrate‐Based Foods • 135
starches into maltose. This conversion stage is known as
mashing. Once the complete conversion of starches into
maltose occurs, the grains are kilned (heated and dried), and
are then ready to be used in brewing.
Malted grains or flours are used to make, in addition to
beer, malt whiskey, and malted milk beverages (such as
Ovaltine), and to provide a malty flavor to candies (malted
milk balls) and baked products (such as bagels).
Lactose (Milk Sugar)
Lactose is derived from milk—hence its other name, milk
sugar. It is one of the few carbohydrates derived from an ani-
mal source. It is a reducing sugar formed from the combina-
tion of the two monosaccharides, glucose and galactose.
Lactose is commercially extracted from whey, a by‐product
of cheese manufacturing. It is most commonly added to food
formulations as a component of dried whey (up to 75 percent
lactose). Whey also contains highly functional milk proteins
that provide multiple benefits to the formulations into which
it is incorporated, including enhanced nutrition, enhanced
binding and foaming properties, and enhanced water‐ holding
properties, along with the browning and dairy flavor notes
from the lactose. Lactose is the least sweet of the commonly
used sugars.
There are large populations of lactose‐intolerant indi-
viduals in the United States and around the world, particu-
larly in or from areas where dairy animals are rare or
nonexistent. These people lack the lactase enzyme required
to break down the lactose molecule to its constituent mon-
osaccharides. This results in gastrointestinal distress, which
can be quite unpleasant. Over‐the‐counter enzyme replace-
ment supplements are available (for example, Lactaid), as
are pre‐treated milks and other dairy products. Fermented
dairy products, such as aged cheeses and yogurt, in which
the lactose is broken down by bacterial fermentation, are
also well tolerated.
Sucrose (Table Sugar)
Sucrose is the standard to which all other sugars are com-
pared. Sucrose is generally called sugar in the United States,
and until the advent of high‐fructose corn syrup (HFCS) it
was the most common sweetener used industrially. Today,
HFCS is preferred (especially in the bottled beverage indus-
try) due to its lower cost and instant solubility. Sugar is
commercially extracted and refined from both sugarcane
and sugar beets. It is a disaccharide consisting of one
molecule each of glucose and fructose; their respective
reactive ends are bound to each other, making this a non‐
reducing sugar—that is, it won’t support Maillard browning.
Sucrose is commercially available in granulated forms from
very large crystalline coarse sugar for high‐visibility coat-
ings, to medium granulation (household sugar), bakers’
special, superfine (or castor), and ultra‐fine particulates,
including confectioner’s sugar. Particle size affects the ease
with which the sugar becomes solubilized (dissolves). Thus
the finer bakers’ special sugar is used in low‐moisture/high‐
fat bakery items because it is incorporated more easily than
the larger granules of household sugar.
Powdered forms of sucrose, known as fondant or confec-
tioner’s sugar, are available in 10X (finest), 6X, and 4X (coars-
est). These products are made from ground crystalline sugar
to which 3 percent cornstarch is often added to inhibit cak-
ing (ground sucrose is strongly hygroscopic). These pow-
dered forms are essential in low‐moisture/high‐fat systems
where granular sugar does not dissolve. In frostings, icings,
whipped cream, and meringues, powdered sugar is used to
achieve a highly sweetened yet smooth texture not attainable
with coarse ground sugars, which cause a gritty mouthfeel.
Light, medium, and dark brown sugars are commercially
made by coating refined white sugar with molasses (3.5 to
6.5 percent, respectively). Molasses is the syrup remaining
after sucrose is extracted from cane or sugar beet juice dur-
ing the refining process. There are also naturally brown‐
colored sugars (turbinado, demerara, muscovado) that are
not refined but rather heated and allowed to crystallize in
the presence of the naturally occurring sugarcane juices
during the initial pressing of the cane. Because these sugars
are not whitened using bone char (the method of choice for
cane sugar), they are considered vegan, unlike standard
brown sugar.
During the sugar‐making process, juice extracted from
sugarcane or sugar beets is boiled until the sugars crystallize
and precipitate out. (As noted above, the syrup left after crys-
tallization is molasses.) Typically, sugarcane juice undergoes
three cycles of boiling and crystallization to extract as much
sugar as possible. With each successive cycle, the leftover
molasses contains less sugar:
• Light Molasses: This is the syrup left over after the first
boiling cycle of sugarcane juice. This molasses is the
lightest in color and has the highest sugar content and
the least viscous texture.
• Dark Molasses: Dark molasses is the by‐product of the
second boiling cycle of sugarcane. This molasses is
darker and more viscous than light molasses, and con-
tains less sugar.
• Blackstrap Molasses: This is the final by‐product of the
third boiling cycle in the sugar‐making process. Black-
strap molasses contains the least sugar and has the
highest concentration of vitamins and minerals. It has a
very dark color and is extremely viscous in texture.
Because this type of molasses is highly concentrated, it
has a deep, pungent flavor.
Molasses is also available as unsulfured and sulfured. The
sulfured variety tends to have a lighter color and flavor.
Sucrose is also commercially available in liquid form as
simple syrup (50 percent sucrose and 50 percent water) and
in custom blends of sucrose and dextrose and sucrose and
corn syrup. These are tailored to the functional needs of the
product or customer and bring a high degree of efficiency
with respect to ease of transport, storage, and use.
c08.indd 135 1/14/2016 7:22:29 AM
136 • CULINOLOGY®
Another liquid sweetener made from sucrose is invert
sugar, made by the hydrolysis of sucrose into glucose and
fructose. Invert sugar is sweeter than the parent sucrose due
to the presence of the sweeter‐tasting fructose, and it is sub-
stantially more hygroscopic, which aids in keeping products
like baked goods moist. It is also used to control crystalliza-
tion in syrups and liquid candy fillings. Invert syrup has been
replaced in most commercial applications by HFCS, which
has similar functionality and better economics.
Honey
Honey is a sweet, syrupy, distinctly flavored sweetener made
primarily by honeybees from the nectar of flowers. The bees
repeatedly ingest and regurgitate the nectar until it is par-
tially digested and takes on the sensory and functional quali-
ties of honey. At this point the honey is placed in unsealed
honeycomb cells, where it is fanned by thousands of bees’
wings until it dehydrates and concentrates to the point where
the water activity is so low (a
w
<0.6) that fermentation cannot
take place. The cells are then capped, and the honey remains
in the hive until needed.
Being a product of nature, the chemical makeup of honey
is variable and depends on the bee’s diet, the types of flowers
in the vicinity, the climate, and the time of year, among other
factors. However, honey is primarily a combination of fruc-
tose (38 percent), glucose (31 percent), and water (17 per-
cent), with several other minor constituents, mainly other
sugars and trace amounts of vitamins, minerals, and assorted
phytochemicals. Honey supplies approximately 3 calories per
gram and can be as much as 1.5 times sweeter than table
sugar, depending on the amount of fructose present. Due to
its low water activity and acidic pH of <4.0, honey has an
extremely long shelf life at ambient temperatures if capped to
keep out moisture. Honey should not be used in infant for-
mulas (under the age of one year) because heat‐resistant
botulism spores may be present that infant digestive systems
cannot defend against.
Interestingly, honey is not considered appropriate for
vegan diets for the same reason milk is prohibited: Both are
considered animal products.
Commercially, honey can be purchased by color, floral
source, and grade (A‐B‐C grading is based on total solids,
water content, flavor, aroma, and clarity). It is available pas-
teurized, whipped, and spray‐dried. It is popular as an ingre-
dient in baked goods, condiments, candies, lozenges, and
beverages, and as a common replacement for table sugar. It is
also used to make mead, a honey‐flavored wine.
Maple Syrup
Maple syrup is made by boiling and concentrating the sap
collected each spring from maple trees. It takes approximately
10 gallons of sap (the average total seasonal production from
one tree) to make 1 quart of syrup.
Canada is the largest producer of maple syrup, and the
United States is the largest consumer. Maple syrup is most
often eaten as a breakfast syrup, but it is also used as an
ingredient, flavoring, and sweetener in candies, baked goods,
hot cereals, ice cream, and other sweet applications.
Maple syrup is approximately 67 percent carbohydrates,
of which the majority is sucrose, and 30 percent water. It is an
excellent source of several minerals, including calcium, iron,
manganese, and zinc, and provides 2.6 calories per gram.
Commercially, maple syrup is available in several grades,
all based on color, density, and flavor intensity. The former
U.S. grades were: A (Light Amber, Medium Amber, and Dark
Amber), B (darker than Grade A Dark Amber), and C
(Commercial Grade). But following an initiative started in
Vermont (the largest producer in the United States) in 2014, in
2015 they were changed to Grade A Golden Color With
Delicate Taste, Grade A Amber Color With Rich Taste, Grade
A Dark With Robust Taste, and Grade A Very Dark With
Strong Taste. The last one is sold for strictly commercial
usage. The new standards are meant to signify more clearly to
consumers what they should expect to taste in a particular
bottle of maple syrup in a way that the previous grading
system didn’t.
Imitation maple syrup is also widely used as a cheaper
alternative. It is typically made from HFCS flavored with
sotolone, a major aroma and flavor component of the
fenugreek seed. At low concentrations, it smells like maple (at
higher concentrations it smells like curry; fenugreek is a
major spice component of curry powders). Syrups that use a
small amount of real maple along with maple‐like flavorings
can be labeled “maple flavored,” but syrups made with no
maple content at all cannot use the word maple in their
names. Instead, they must be labeled “pancake syrup,” “waffle
syrup,” or other fanciful names.
Agave Nectar
Agave nectar is a natural sweetener from the same Mexican
plant from which tequila is made. It comes in three varie-
ties: light, amber, and raw. While agave is thinner than
honey, it is slightly thicker than simple syrup. Agave syrup is
composed mainly of fructose, so it shares the same sweet-
ness level—approximately 1.5 times sweeter than sucrose. It
also shares the same relatively low glycemic index of fruc-
tose. Agave is not calorie‐free—it contains 20 calories per
teaspoon, 5 more than granulated sugar—but because it’s
sweeter than sugar, less is needed to achieve the same level
of sweetness.
Previously, agave syrup was the preferred sweetener of
the healthy eating crowd, but recent reports have suggested
that a diet high in fructose (and agave syrup has the highest
level of fructose of any sweetener, even more than high-
fructose corn syrup) can lead to an assortment of unhealthy
outcomes, including weight gain (especially belly fat) and
childhood obesity, insulin resistance, increased risk of meta-
bolic syndrome and heart disease, among others. Limiting
intake of fructose in such a concentrated form is now as
strongly recommended as reducing intake of high-fructose
corn syrup.
c08.indd 136 1/14/2016 7:22:30 AM
CHAPTER 8 • Carbohydrate‐Based Foods • 137
Polyols
Polyols, also known as sugar alcohols, are carbohydrates, but
they are neither a sugar nor an alcohol. Being sweet but not
being a sugar qualifies them as sugar‐free, and they are often
used in formulations using the “no sugar added/reduced sugar”
nutritional claim. Most polyols are less sweet than sucrose and
have a lower caloric content. Most of them are suitable for
products designed for diabetics because they cause low to no
insulin response on ingestion. Polyols uniquely have a negative
heat of solution, which explains why many of them evoke a
cooling sensation as they dissolve in the mouth. They are also
non‐cariogenic (do not cause cavities) and are therefore widely
employed by manufacturers of sugar‐free products. They are
only partially absorbed in the small intestine; hence, they enter
the large intestine intact. In the lower gut, polyols can cause
flatulence and laxation similar to non‐absorbed, fermentable
carbohydrates (in varying degrees, depending on the polyol
and differences in individual responses).5
Sorbitol is a naturally occurring sugar alcohol, although
most is commercially manufactured by hydrogenating dex-
trose. It has a sweetness intensity value of 60 (sucrose = 100),
high solubility in water, and can develop high viscosity in
solution. It provides mouth‐cooling and has a low propensity
to participate in browning reactions. Because of its hygro-
scopicity, sorbitol has good humectant properties, retaining
moisture in baked goods, which prolongs freshness, and
reduces water activity by binding free water molecules and
reducing their ability to participate in chemical reactions,
thereby extending shelf life. This is important in intermediate
moisture products, such as breads and flour tortillas, which
support mold growth when no humectants are used. Sorbitol
is suitable for incorporation into products for diabetics. It is
often used in reduced‐calorie or sugar‐free cakes, candies,
and chewing gum.
Erythritol is commercially produced from fermentation by
the yeast Monilliela pollinis. It has an extremely low caloric
value and a relative sweetness value of 60 to 70 percent of
sucrose. It is often used as a sugar replacer in candy and bak-
ery products where the bulk of the sugar must be replaced.
Much of it is absorbed in the small intestine and thus does not
promote the gastric distress seen with many of the polyols.
Erythritol does not crystallize, nor is it hygroscopic, and thus
it is often used in combination with other sugar replacers to
overcome these shortcomings. It has a cool, minty aftertaste.
Isomalt is used extensively in the manufacture of sugar‐
free hard candy due to its excellent crystallization properties
and its compatibility with traditional hard candy manufac-
turing lines. It has a sweetness intensity of 50 to 60 when
compared to sucrose, and no cariogenic potential. It has low
solubility in water, low tendency to brown when heated, and
a minimal cooling effect on dissolution.
Lactitol has a sweetness intensity of 30 to 40 when com-
pared to sucrose. It has no cariogenic potential, is highly suit-
able for diabetic products, and does not brown when exposed
to heat. It has little aftertaste, which makes it ideal for appli-
cations such as chewing gums, chocolates, and jams.
Maltitol has good heat stability and low hygroscopicity,
resists microbial breakdown, and displays moderate solubil-
ity in water. Its sweetness intensity is 90. Maltitol is used in
baked goods to retain moisture and in beverages and confec-
tionery products as a sweetener.
Mannitol is a naturally occurring sugar alcohol with a
sweetness intensity value of 50. It exhibits a pleasant, low
mouth‐cooling effect. It exhibits low water solubility and it
does not brown.
Xylitol is a naturally occurring sugar alcohol that finds its
main application in sugar‐free gums due to its negative cari-
ogenic potential and its sugar intensity value of 100. It dis-
plays high water solubility, low propensity to browning, and
high mouth‐cooling effect.
High‐Intensity Sweeteners
High‐intensity sweeteners, as their name suggests, provide
many times the sweetness of standard sucrose. Most are calo-
rie free, but even those that provide calories are used at such
low levels that their caloric effect is negligible. Because they
are used at low levels, they are often combined with malto-
dextrins or polyols to replace the bulk and functional gap cre-
ated by the absence of sugars.
These sweeteners can be divided into two camps: synthet-
ics and naturals. Of the synthetics, only Suralose (bulked and
sold at retail under the Splenda® brand in yellow packets) is
derived from a carbohydrate (chlorinated sucrose); it is about
600 times the sweetness of table sugar. It is heat‐ and low pH‐
stable, which makes it a good choice for heat-processed or
acidified foods.
Of the naturals, stevia, extracted from the leaves of an
herb in the sunflower family and native to Asia and South and
Central America, provides the highest intensity at 300 times
the sweetness of regular sugar. The taste of stevia is said to
have a slower onset and longer duration than that of sugar.
However, some of its extracts may have a bitter or licorice‐
like aftertaste at high concentrations. Stevia was approved by
the FDA for use in foods in 2008.
Luo han guo is a fruit native to southwestern China that
has been used for centuries as a natural sweetener, medicinal,
and longevity aid. It is about 250 times sweeter than sugar and
dissolves well in beverages. There are no reported incidents of
negative side effects. It is classed by the FDA as a GRAS prod-
uct, with no restrictions on consuming the fruit or its extracts.
The extract is currently used in this country as a sweetener in
natural cereals, herbal teas, and low‐calorie juice drinks.
Starches
When we consider starch as an ingredient, we think of
adding it to meat drippings and then stirring over heat
while waiting for it to thicken. We think of the gravy boat
in the refrigerator the day after—the gelatinous mass with
the split in the top surrounded by a pool of liquid. Have
you asked why?
c08.indd 137 1/14/2016 7:22:30 AM
138 • CULINOLOGY®
Have you ever wondered why the pie filling made from
fresh fruit is firm, dull, and opaque but that from a can or in a
store‐bought pie is flowing and shiny? The answer to this
question is starch.
Although starches are most often used for thickening, they
are also used to provide adhesion (batters and breading), film
formation (nut and snack coatings), moisture retention
(meats and bakery applications), glazes (toppings), dusting
(confections and bakery), and stabilization (fermented dairy
and frozen desserts). In most applications, starches are easy
to work with and inexpensive relative to the role they play in
a formulation.
Starches are generally white to off‐white in color. Most are
not soluble in water but rather form dispersions. Stirring a
spoonful of starch into a cup of water yields a white, opaque
liquid. On standing, the starch settles to the bottom. Starches
are considered tasteless, but “bland‐tasting” might be a bet-
ter description, and if not completely “cooked out,” they will
leave a powdery mouthfeel when consumed.
Starches are produced by green plants as a highly compact
means of storing energy. Depending on the plant, starches
can be found in the stem, leaves, root, fruit, or seeds. The pre-
dominant source in the United States is corn (seed), with
wheat (seed), rice (seed), and potato (root) having some
significance. Each source of starch may require a different
separation process to extract the starch portion for human
use from the remainder of the plant, but regardless of the
botanical source or the refinement process, starch is made
up of multiple glucose molecules joined in a linear fashion
like the links of a chain. Picture this chain coiled like a spring,
1000 to 10,000 glucose units in length, and you have the linear
starch molecule called amylose. A second molecular form of
starch is amylopectin, which is also helical, but it has much
longer chain lengths of 300,000 to 3,000,000 glucose units and
branch chains as well.
Starches from any source are made up of amylose and
amylopectin, and it is the ratio of these two molecules and
their different molecular structures that give each starch its
characteristic functional properties.6 In nature, the majority
of starchy foods contain a mixture of 75 percent amylopectin
and 25 percent amylose.
These two structures are synthesized by the plant and
built into a highly compacted form called a starch granule.
The size and shape of the starch granules are particular to
each plant source, but all are extremely small and measured
in microns. The length across the head of a pin is approxi-
mately 1 millimeter, which is 1000 microns. Potato granules
are the largest of the common food starches, and they meas-
ure only 100 microns. The smallest starch granules are from
rice and are measure 1 to 3 microns.
Starches are often compared to each other and their suit-
ability for a particular application by looking at their viscosity
profile: a measurement of their thickening power over a given
temperature range. A reproducible viscosity profile can be
H
O
H
H
H
OH
OH
OH
H
CH2OH
H
O
Glucose
(Dextrose)
Amylose
(linear)
Amylopectin
(branched)
H
H
OH
OH
H
CH2OH
H
OH
O
H
H
OH
OH
H
CH2OH
OH
O
H
O
H
H
OH
OH
H
CH2OH
H
OH
O
H
H
OH
OH
H
CH2OH
OH
H
H
OH
OH
H
CH2
OH
O
H
O
H
O
H
H
OH
OH
H
CH2OH
H
OH
O
O
FIGURE 8.10 Molecular structures of starch.
c08.indd 138 1/14/2016 7:22:30 AM
CHAPTER 8 • Carbohydrate‐Based Foods • 139
created from a starch‐water dispersion of known starch con-
centration as it is heated, cooled, and mixed at controlled
rates at targeted temperatures. The two instruments most
commonly employed for this job are the Brabender Visco-
amylograph (Brabender) and the Rapid Visco Analyzer (RVA).
Both measure and record the apparent starch viscosity via
its resistance to torque (a twisting force that tends to cause
rotation) generated by the instrument’s rotating paddle or
spindle. The profiles generated by both the Brabender and
the RVA show the initiation of viscosity as the starch slurry
reaches its gelatinization temperature.
Gelatinization is the point where starch granules begin to
hydrate and swell, and an increase in viscosity of the slurry is
observed. Some form of energy input is required to weaken
the crystalline structure of the granules in order for water to
enter the granule. Heat and mechanical shear (stirring is the
most common form of shear energy) are the usual forms of
energy applied to make the granules absorb water and begin
swelling.
During the heating phase, some amylose leaches from the
granules into the water because the amylose molecules are
relatively small and unbranched, and thus diffuse more easily
out of the granules. The combination of the water‐bonding
character of the leached amylose molecules and the increased
friction between the swelling starch granules gives starch
its thickening ability. Viscosity is a function of the internal
friction of a fluid—its tendency to resist flow. Increasing vis-
cosity thus is a result of an increase of internal friction due
to the reduction of free movement of the dispersed solids
from the enlarging granules and to the continuing seepage
of amylose into the liquid. As heating continues, more of the
starch granules swell and enlarge until the majority are fully
hydrated. On the viscosity profile, a point of peak viscosity
is noted.7
Confusion exists in the starch industry, as some people
use the term pasting temperature to refer to the gelatiniza-
tion temperature while others use it to indicate the point of
peak viscosity. Pasting actually starts as gelatinization of the
granule begins and continues until peak viscosity is reached.
At this point the starch is said to be pasted.
Many profile‐testing protocols are programmed to hold
at a high temperature for a prolonged time in order to evalu-
ate the starch’s stability under conditions of extended heat-
ing and mechanical shear. Appropriately enough, this is
called the hold phase; it mimics conditions of extended
thermal processing typically used in industry. Pasted, fully
swollen, hydrated granules are highly susceptible to shear.8
Starch granules from many of the native unmodified
starches begin to break from the constant agitation during
this phase. As the granules fracture, a resultant reduction of
viscosity is observed. Viscosity stability may be assessed by
comparing peak viscosity to the viscosity measured at the end
of the heating phase of the profile. A reduction of viscosity is
called breakdown.
A cooling phase usually follows the high‐temperature
hold. The term for the general increase of viscosity seen
during this phase is retrogradation or setback; the higher the
viscosity in the cooling phase, the more setback a starch
exhibits. The increase of viscosity is due to the reassociation
or realignment of the amylose molecules leached from the
granules during the heating phases. A good example of amyl-
ose retrogradation is the gravy boat in the refrigerator the
day after Thanksgiving, where the creamy‐smooth and flow-
ing gravy has solidified into a gelled mass. Amylopection mol-
ecules also reassociate, but at a much slower rate. An example
Heating
Cooling 50°C
Hold
95°C
Hold
Pasting
Viscosity
(cps)
0 10
50°C 95°C 95°C 50°C 50°C
20 30 40 50
Time (Minutes)
60 70 80 90 100 110 120
Peak Viscosity
Gelatinization Temp
Breakdown
Setback
FIGURE 8.12 Starch viscosity profile.
FIGURE 8.11 Starch granule hydration.
Pasting
Peak
Viscosity
G
e
la
ti
n
iz
a
ti
o
n
D
egradation
Viscosity
Temperature
c08.indd 139 1/14/2016 7:22:30 AM
140 • CULINOLOGY®
of amylopectin retrogradation is bread staling. After baking
and cooling, these branched chain molecules retreat from
their dilated state to a more compacted state as staling pro-
gresses, resulting the hardening of the crumb structure over
time.9
A microscope can be used as an excellent quality control
(QC) tool in a food manufacturing facility to evaluate starch
efficacy. A slide can be easily prepared from a starch slurry
or a food containing starch. A drop of 5 percent iodine solu-
tion on the sample dyes the starch granules, which can be
viewed using a microscope at 200 times magnification. It
becomes obvious if the granules are gelatinized, partially
swollen, fully pasted, or even to the point of fracturing.
Microscopy can be used during processing as an operations
tool to ensure the starch granules reach optimum hydration,
often referred to as being cooked out. This technique can also
be implemented later as a quality check on finished products.
With a little experience one can determine the source of the
starch by its characteristic size and shape as well as the stage
of granule hydration.
Native Starches
Starches are commercially available from numerous plant
sources, most commonly corn (maize), cassava (tapioca),
wheat, potato, and rice. Pure starches from all sources are
almost entirely digestible. Protein and fat content of starches
is negligible. Starches extracted directly from the plant
source without modification (chemical or physical treat-
ment) are simply termed starch, unmodified starch, or native
starch, and are considered a natural ingredient. Starches
from cereal grains have higher gelatinization temperatures
and correspondingly higher temperatures for peak viscosity
than those from roots or tubers. Potato and tapioca starches
cook out much faster than corn, wheat, or rice starches.
Starches from grains produce gels that are short‐textured,
firm, and opaque, while those of potato and tapioca are usu-
ally gummy and more pliable.
Native starches, although excellent for many food appli-
cations when prepared and consumed quickly, tend not to
have functional stability suitable for large-volume process-
ing, extended heated holding times, low-pH environments,
retorting, long‐term refrigerated storage, or freeze/thaw sta-
bility. Using native cornstarch to prepare gravy is a good
illustration. The gravy may be superb when prepared
immediately before serving, but hold it on a steam table and
over time the gravy thins. This change of viscosity is due to
the deterioration of the starch granules from the constant
input of heat energy. Starches with ruptured granules or even
intact granules that have leached a large degree of amylose
show lower viscosity than the same starch with intact, fully
swollen granules.
If the same gravy is refrigerated overnight, the next day it
appears as a gelled mass with perhaps a split or two in the gel
and water separating out. This is the result of starch
retrogradation—the realignment and reassociation of the
amylose molecules. Retrogradation of amylose molecules
reduces the spacing between the molecules, which actually
squeezes water out of the gel matrix. As the starch molecules
become more tightly packed, the system becomes denser,
more opaque, and less shiny, and syneresis (weeping) occurs.
Retrogradation of amylose can be reversed, although tem-
peratures as high as 239 to 248°F (115 to 120°C) may be
required. Retrograded amylopectin gels are softer and are
more easily reversed, with heating temperatures of only 122
to 185°F (50 to 85°C) required.10 The long, stringy texture of
retrograded amylopectin is relatively unappealing for most
food systems.
Culinologists developing products for foodservice or
retail applications must consider the conditions these foods
encounter not only in processing and packaging but at
every step in distribution, marketing, and consumption as
well. Consider the gravy described above. The gravy may be
heat‐processed in a steam‐jacketed kettle and pumped
across a factory floor to the filling area. Both heating and
pumping add stresses to the swollen starch granules, which
increases their propensity to fracture. This gravy might then
be hot‐filled and boxed without cooling. Once packaged,
stack heat, or heat retained in the product, can remain
entrapped for days and continue to stress the starch sys-
tem. Alternatively, this gravy might be pouched and
frozen—another temperature stress on the formulation.
FIGURE 8.13 Native starch granules.
Patato
Waxy Corn
Corn
Wheat
FIGURE 8.14 Microscopy of starch granule
hydration.
Uncooked
Fully
Hydrated
Partially
Hydrated
Starch
Fragments
c08.indd 140 1/14/2016 7:22:32 AM
CHAPTER 8 • Carbohydrate‐Based Foods • 141
High heating temperatures combined with refrigerated or
frozen storage actually induce retrogradation faster than
room‐temperature storage.
Thermal energy is not the only form of stress applied to a
starch system in a food formulation. Shear is a form of
mechanical energy input and can affect granule hydration
and, perhaps more importantly, granule fracturing. Shear is
introduced via the common industry practices of pumping,
mixing, and homogenizing.
Another form of stress is high acidity (low pH). Formula-
tions with high acid content affect granule hydration and
fracturing during processing as well. In cherry pie filling, for
example, the high acid content of the cherries might cause
the starch granules to fracture and thus induce a loss of thick-
ening at a temperature considered optimum for a more neu-
tral pH system, like the gravy. The functional limitations of
native starches in commercial food processing explain the
need to use modified starches, which are designed to survive
in these complex systems.
Chemically Modified Starches
All modified starches are not created equal, as each modifica-
tion is designed for a specific purpose and function. The most
common application for starch is thickening, and therefore
the most common modifications are those that enable the
starches to withstand the common stresses from processing—
heat, acid, freezing and shear—and retain thickening power.
Starch modifications are also made to improve the tempera-
ture of gelatinization, hot viscosity, and gel strength. Others
improve adhesion of one food to another or simply make the
starch appear whiter. Although any native starch can be
modified, the most commonly used in the United States is
corn. The chemicals used for food starch modification, their
respective levels, and their combinations thereof are out-
lined in Title 21 of the Code of Federal Regulations,
Section 172.892.
Cross‐Linked Starches
Cross‐linked starches are treated with a chemical that forms
bridges between the starch molecules. This cross‐linking
makes the granules more resistant to gelatinization as it
inhibits swelling. This inhibition is directly proportional to
the amount of cross‐linking. As a cross‐linked starch is
heated, peak viscosity can actually be higher than that of the
native starch as the starch granules are more resistant to
both fracture and amylose leaching throughout the stress of
continuous cooking. Therefore more granules remain intact
and do not fracture as others continue to swell and add vis-
cosity to the system. A highly cross‐linked starch may never
develop a peak viscosity on an RVA or Brabender viscome-
ter if the granules are over‐inhibited and resist swelling
under the viscometers’ limited operating temperatures.
This high degree of cross‐linking is designed for retorted
foods where temperatures of 250°F (120°C) are reached and
early viscosity development is a disadvantage because it
slows heat penetration.
Cooking starch in an acidic environment often results in a
rapid viscosity decline. Again, cross‐linking can ameliorate
this phenomenon by strengthening the bonds between the
starch molecules.
Viscosity stability is not the only benefit from cross‐ linking,
as paste texture is also affected. Keeping the starch granules
intact allows for a shorter, smoother texture versus a texture
considered long, gummy, or stringy.
Substituted Starches
Pasted native starches retrograde with time, changing the
texture from a paste to a firm paste or even a rigid gel, if the
particular starch has significant amylose content. As retro-
gradation progresses, the starch’s appearance changes from
clear or translucent to opaque. Starch manufacturers can
modify native starches using a substitution reaction. This
process binds a chemical to the starch molecules, creating a
larger, bulkier molecular structure. By doing this, the amylose
molecules can no longer reassociate as closely as before. A
good analogy is the live Christmas tree wrapped in twine as
it arrives at the store; remove the twine, and it is no longer as
compact as it was when shipped. Because the molecules
cannot realign as tightly, the paste retains a more smooth
and creamy texture versus the cuttable gel indicative of ret-
rograded amylose, and it retains the clarity seen in the pasted
sol (a semisolid dispersion). Along with the sustained clarity
comes a reduction of syneresis; the starch molecules are
unable to fit closely together, so water is not squeezed from
the system. Substitution gives long‐term stability to a starch‐
containing food—especially important in refrigerated and
frozen food applications (such as TV dinner gravies).
Substitution has a secondary benefit: The energy needed
to gelatinize these bulky molecules is reduced. Imagine a
wedge splitting firewood. As the wedge is driven into the
grain of the wood, initially the log separates only enough to
allow the wedge to penetrate—until that last tap with the
maul, when the entire log abruptly splits apart. Substitution
creates an internal force in the granule so less external energy
is needed for it to expand. The effect is that the substituted
starches begin to hydrate and reach optimum hydration (and
viscosity) earlier in the cooking process than would the native
starch from which it was made.
Stabilized Starches
The term stabilized starch is generally understood to mean
modified starches that are both cross‐linked and substituted.
Dual‐modified starch is another common term for the same
thing. Stabilized starches are most commonly used for appli-
cations where a clear, heat‐ and perhaps acid‐tolerant freeze‐
thaw–stable thickener is needed. A classic example is a
cherry pie filling. Compare two fillings made with different
starches:
c08.indd 141 1/14/2016 7:22:33 AM
142 • CULINOLOGY®
starch, sugar, and flavor) to be poured into molds, where they
cool and set back to a firm gel. Terms such as thin‐boiling and
acid‐thinned starches are also used for these products.
Physically Modified Starches
Native starches can be subjected to treatments, such as heat
and pressure, without the use of chemicals; these physical
treatments also affect gelatinization and pasting characteris-
tics. They enhance the functional stability of the starch but
have yet to match the results developed with chemically
modified starches. Another downside is that currently they
are about double the cost of chemically modified starches.
However, they should be considered when a food formulation
with a “clean” label is required, as these starches can be
labeled “natural.”
Pre‐Gelatinized Starches
The term pre‐gelatinized starch is synonymous with pre‐
gelled starch and instant starch, as they are commonly known.
Various methods may be employed to create a starch that
thickens, without heating, almost immediately as it makes
contact with water. The oldest and most common method is
drum‐drying. This process applies a starch slurry onto a
rotating cylinder (drum) where the starch is heated, pasted,
and then dried. The cooked, dry starch is scraped from the
drum and ground to a powder. Drum‐drying a stabilized
starch produces a starch with some degree of heat, acid, and
shear stability, but the process often fractures the pasted
granules and results in an instant starch of reduced viscosity
and stability compared to its parent cook‐up starch.
Other proprietary technologies are utilized to pre‐
gelatinize that leave the granules intact through the process.
One such procedure is a spray‐cook process whereby the
starch slurry is heated, swollen, and dried in seconds through
a special spray‐drying nozzle. Another technology utilizes
heating of the starch in a high concentration of alcohol.11
Both of these, which maintain the intact granule, are termed
cold‐water swelling starches (CWS) and tend to have viscos-
ity, texture, and stability similar to their cook‐up counter-
parts, but the cost can easily be double.
The most familiar use of an instant starch is instant pud-
ding mix. By adding milk to the dry mix, thickening is immedi-
ate. A less‐known application is a layer cake designed for frozen
storage. Selection of the appropriate CWS starch provides the
correct batter consistency to avoid the formation of large air
pockets yet offers moisture management in the cake through-
out its storage, reducing shrinkage and maintaining texture.
Starch Selection
When considering the number of primary native starches
commercially available as well as the number of modifica-
tions, degrees of modification, and potential combinations of
modifications, one begins to realize how many starch options
are available. The research chef or food technologist should
use the expertise of the starch supplier to help select the best
starch for each application. The supplier must know the
Cherry Pie Filling
Ingredients for Each Version
Water 90 g
63 DE corn syrup 110 g
Sugar 83 g
Lemon juice 5 g
Salt 1 g
IQF cherries 500 g
Prepared 9‐inch pie shells 2
For Version 1, use 31 g unmodified cornstarch; for Ver sion 2,
use 31 g stabilized, bake‐stable cornstarch.
Procedure:
1) Prepare 2 fillings by mixing 83 g sugar, 110 g corn syrup,
1 g salt, 5 g lemon juice, and 100 g cherries (including
liquid from cherries, if any) into each of two heating
vessels.
2) Prepare two slurries, mixing each starch in 90 g water.
3) Add one starch slurry to each pot and heat, stirring, to
190°F (87°C), or until a light boil is observed.
4) Add the remaining 400 g cherries to each vessel and
carefully blend into the mixture to prevent damaging the
fruit.
5) Fill one 9‐inch pie crust with each mixture.
6) Bake at 425°F (218°C) for 15 minutes. Reduce the heat
to 400°F (204°C) and bake another 30 minutes.
7) Compare after cooling. The pie with the native starch is
pasty and dull, with a high gel set. The pie with the
modified starch is shiny, and the syrup component has a
nice flow.
Oxidized Starches
Starches are subjected to oxidation reactions for two main
purposes: whitening and promoting adhesion. When a lower
level of oxidation is used to whiten, these starches are termed
bleached starches. A higher degree of oxidation is used to pro-
duce modified starches that have a lower gelatinization tem-
perature, reduced paste viscosity, and improved clarity
compared to the native starch from which it was made. The
largest food use is in the batter and breading industry, where
specialized oxidized starches are added to a batter formula-
tion to promote the adhesion of the batter to the substrate.
Acid‐Hydrolyzed Starches
Starches subjected to an acid treatment, such as hydrochlo-
ric acid, are called acid‐hydrolyzed starches. This process,
considered a form of starch conversion, cleaves the starch
molecules into shorter segments yet retains the granular
structure of the starch. Acid‐converted starches uniquely
have a low hot‐paste viscosity, which allows the use of higher
starch levels in a formula, yet set back to extremely firm gels.
The confection industry takes advantage of these properties
in the manufacture of gummy candies. The thinner hot
viscosity allows the high‐solids liquid (acid‐hydrolyzed
c08.indd 142 1/14/2016 7:22:33 AM
CHAPTER 8 • Carbohydrate‐Based Foods • 143
functional expectations needed from the starch as well as the
handling and processing parameters to which the food will
be subjected. Remember the conditions for gelatinization
and pasting: heat, water, mechanical action. Remember the
conditions of stress: heat, acid, shear, and time. Add to those
the fluctuations of temperature with freeze‐thaw.
Many suppliers offer a specialized starch for a particular
industry, such as bakery, meats, fruit fillings, or sauces. Many
manufacture specialty starches for a particular application,
such as emulsification, aqueous‐based coatings, retort appli-
cations, and confections. Ask the experts at your starch sup-
plier to select the best option for you.
Converted Starch Products:
Maltodextrins and Corn Syrups
Converted starch products is a collective term reserved for
those materials manufactured through the hydrolysis (decom-
position of a chemical compound by reaction with water) of
the starch molecules. Common methods of starch hydrolysis
(also known as depolymerization) are exposure to heat, acid,
and enzymes singularly or in combination.
Pyroconversion (conversion by heat) of an acidified starch
produces a group of converted products collectively called
dextrins. Older technology produces dextrins that are
strongly flavored and often yellow to brownish in color.
Newer technology employing a combination of heat, acid,
and enzymatic hydrolysis produces maltodextrins, which
generally form clear solutions in water, are not sweet relative
to sugar, and have little to no flavor.
Maltodextrins, and the more highly converted (hydro-
lyzed) corn syrup and corn syrup solids, are differentiated by
their dextrose equivalent (DE) value, which is the proportion
of reducing‐sugar content expressed as dextrose content on a
dry basis. DE is deduced via chemistry to provide a number
reflective of the degree of starch molecule hydrolysis—that is,
how much of the starch is broken down to glucose (aka dex-
trose) molecules. The higher the DE value, the greater the
degree of hydrolysis and therefore the greater the amount of
sugars present. Maltodextrins are between 5 and 20 DE and
are not sweet; corn syrup solids (powders) are 20 DE and
above and thus range from somewhat sweet to sweet. Corn
syrups (liquids) come in three grades: low DE is less than 30,
regular DE is 42 (about 40 percent the sweetness of table
sugar) and high DE is 68 (about 60 percent the sweetness of
table sugar).
Like starches, maltodextrins are chains of dextrose (also
called glucose) units, but they are much shorter in length. The
FDA defines maltodextrin as a “non‐sweet nutritive (digesti-
ble) saccharide (sugar) polymer consisting of glucose (syn-
onymous to dextrose) having a DE less than 20.” Maltodextrins
are prepared as a white powder or concentrated solution by
the partial hydrolysis of starch with safe and suitable acids or
enzymes. They have GRAS status as direct human food
ingredients at levels consistent with current good manufac-
turing practices (21 CFR 184.1444).
Maltodextrins are widely used to add body and mouthfeel
without additional flavors or sweetening. Often, adding sol-
ids to a formulation contributes to the body and texture of
that food. An excellent illustration is to compare the mouth-
feel of a regular sugar‐sweetened beverage, like lemonade, to
an artificially sweetened version. Absence of the sugar solids
changes the body of the diet drink to a more watery consist-
ency. Much of the technology for successfully marketed low‐
fat foods revolves around replacing the solids lost in the
formulation with maltodextrin. In so doing, the differences of
body and texture between the two formulations are mini-
mized, and the calories are greatly reduced (fat = 9 kcal/g;
maltodextrin = 4 kcal/g).
Maltodextrins are also used as carriers or fillers to bulk up
(dilute) highly concentrated flavors and colors, making them
easier to measure and disperse into a food system. They are
widely used as bulking agents for diluting high-intensity
sweeteners, so that, for instance, a packet of Nutrasweet® or
Chemical Modification Physical Modification
Function of
Modification
Acid
Hydrolysis Oxidation Crosslinking Substitution Stabilization Pregelatinization
Heat
Treatment
Gelatinization
temperature
X X X X X X X
Viscosity X X X X X
Stress
tolerance
X X
Inhibit
retrogradation
X X
Whitening X
Adhesion X
Film forming X
FIGURE 8.15 Functions of starch modification.
c08.indd 143 1/14/2016 7:22:33 AM
144 • CULINOLOGY®
Splenda® delivers the same level of sweetening as the same size
packet of sugar. If the packet was filled with pure aspartame or
sucralose, it would blow your head off ! They are also used as a
bulking agent in dry mixes to enable a number of recipes to fit
into a single standardized package size. Use of maltodextrins in
these dry blends also helps keep them free‐flowing, as they are
not as hygroscopic as higher‐DE corn syrup solids or sugars.12
Corn syrups are defined as concentrated aqueous solu-
tions of nutritive saccharides obtained from starch with a DE
of 20 or more; thus, their molecular size is smaller than
maltodextrins. The most common in commerce are 36 DE,
42 DE, and 63 DE. The higher the DE, the higher the free glu-
cose content, and therefore the sweeter the taste and the
lower the viscosity.13 Corn syrup solids are the dried equiva-
lents of the syrups from which they are derived. Most often
these products are blended with other sweeteners, such as
sucrose, to add solids to a formulation. This not only contrib-
utes to sweetness but also adds hygroscopicity, changes the
texture, lowers the freezing point, and increases viscosity.14
Functional properties and relative sweetness of the malto-
dextrins and corn syrup solids are shown in Figure 8.16.
The converted starch products with the most notoriety
today are the high‐fructose corn syrups (HFCS). In commercial
production, HFCS is made by first enzymatically hydro lyzing
cornstarch to glucose syrup and then, in a second enzymatic
process called isomerization, converting 42 percent of the glu-
cose to fructose. (Note that HFCS contains both glucose and
fructose—reducing sugars that can participate in Maillard
browning in the presence of amino acids and heat.)
Refining this 42 percent HFCS to obtain a 90 percent fruc-
tose HFCS and then blending it back with 42 percent HFCS
produces what has become the most commonly used HFCS:
55 percent fructose HFCS. The popularity is due to this
combination of traits: It has the same sweetness as cane or beet
sugar at half the cost. The most common usage for 55 HFCS is
in the soft drink industry. Other foods like baked goods, soups,
and salad dressings take advantage of the even less expensive
sweetness of the 42 percent HFCS. The controversy surround-
ing the HFCSs revolves around their high usage rate in foods
and its correlation to the increase of obesity, diabetes, and
other diet‐related health issues facing the public today.
Gums
While starches, flours, and sugars are commonly used in home
and restaurant kitchens, gums are not nearly as common.
Most gums are utilized in industrial food manufacturing.
Only recently have gums, primarily guar gum and xanthan
gum, been available in retail markets. One reason is that
gums are useful in a gluten‐free diet, specifically in making
gluten‐free breads. Many grains, including wheat, barley, and
rye, contain the protein gluten, which is essential to bread‐
making because it helps doughs develop by adding viscosity
and elasticity and, most importantly, by trapping the CO
2
gas
(carbon dioxide) developed during fermentation that allows
the dough to rise. When gluten is removed, dough loses this
ability. Gums can replace some of those attributes, allowing
for the baking of some fairly good gluten‐free breads.
Most gums are as natural as starches and sugars, and
come from plant sources. Just as wheat flour comes from the
endosperm (inside) of the wheat seed, guar gum comes from
the endosperm of the guar seed. (The guar seed and pod look
very much like a pea seed and pod.) Using gums is really not
much different from using starches in terms of naturalness
and health (except that unlike starches, they are virtually cal-
orie free). But the functionality of gums is quite different from
starches and adds a new repertoire of textures and function-
alities to your food creations.
Source: Gum Technology Corporation.
FIGURE 8.16 Functional properties of
carbohydrates as related to dextrose equivalence.
Binding
Browning Reaction
Hygroscopicity
Solubility
Sweetness
Viscosity
Freezing Point
Depression
Functional Property
Corn Syrup SolidsMaltodextrin
Starch Dextrose
20 1000
Dextrose Equivalence (DE)
Sweetener Relative Sweetness
Sucrose (sugar) 100
Fructose 110–170
Glucose/dextrose 60–90
90 HFCS 120–160
55 HFCS 100–110
42 HFCS 90–100
63 DE corn syrup 60–70
42/43 DE corn syrup 40–50
36 DE corn syrup 30–40
20 DE corn syrup solids 20–25
18 DE maltodextrin 18–22
15 DE maltodextrin 15
10 DE maltodextrin 10
5 DE maltodextrin 5
FIGURE 8.17 Relative sweetness of converted
sweeteners.
c08.indd 144 1/14/2016 7:22:33 AM
CHAPTER 8 • Carbohydrate‐Based Foods • 145
Gums are hydrocolloids. Hydro (like hydrate in carbohy-
drate) means “water,” and a colloid is a dispersion in which
one ingredient (the dispersed phase) is evenly distributed
throughout another ingredient (the continuous phase). In
this case, the gum is distributed throughout the water. The
gum particles stay suspended in the water (or other aqueous
medium) because they are so small they do not settle out. At
the same time, they are too large to simply go into solution,
as salt or sugar would. It is the relatively large size of these
molecules that allows them to develop viscosity and gel
systems.
Note that chewing gum is not a gum; it is a resin. Resins
are compounds, derived naturally or synthetically, that do not
dissolve or disperse in water but do dissolve in non‐aqueous
liquids such as oil and alcohol; pine sap is a common resin.
Chewing gum fails the first test of being a hydrocolloid: It
does not disperse in water. If it were really a gum, it would
melt in your mouth and would not provide a pleasant chew-
ing experience. By the same token, it is important to remem-
ber that true gums not only like water but require it or other
aqueous liquids to function. Gums are not soluble in liquids
other than water or those based on water. (Note: From this
point on we use the term soluble or gum solutions because
most people commonly refer to the dispersion of gums in
water as “being soluble.” However, they do not form true solu-
tions; they form colloidal dispersions or suspensions.)
In a true solution, the solute and solvent become one
homogenous material. In a colloidal dispersion there is no
solute and solvent because the solute (in this case the gum) is
immiscible (doesn’t dissolve) in the solvent (the water). If you
could look under a powerful microscope, you would see the
gum particles are actually floating (dispersed) in the water
and are not one with it. On the other hand, if you add gums to
oil, they eventually settle out and do not provide viscosity or
other functional benefit. The same holds true in alcohol, pro-
pylene glycol, and high‐solids sugar solutions. Gums need
water; without water, gums are essentially nonfunctional.
High‐solids sugar solutions, such as corn syrup, do contain
water, so why won’t gums work in them? The answer is that all
of the water molecules are already bound to sugar molecules;
no free water is available to bind with the gum. We can catego-
rize this concentrated sugar solution as having extremely lim-
ited water activity (aw). Most sugar syrups, and honey, are
good examples of foods in which water is present but not
available for microbial organisms to feed on (which is often
our main concern with water activity) or to react with ingredi-
ents that require water, such as gums. Although a typical syrup
might contain 35 percent water (and 65 percent sugar), every
molecule of water is attached to a molecule of sugar and
therefore not available. This binding is the reason honey, jams,
syrups, and strong, high‐salt pickling brines can remain on the
shelf without spoiling even without preservatives.
Please note that gums are not very useful in controlling
water activity. Many people assume that because gums
absorb quite a bit of water (some gums, such as konjac, hold
up to 200 times their weight in water), they control water
activity. This is not the case, as most of the water picked up by
the gum is not bound; it is still available to react with other
materials or for microbes to feed on.
Gums do have the advantage of being functional at very
low use levels. This is not only an important factor when
determining the costs involved in the choice of a stabilizer but
also means they do not mask flavors. Gums tend to be bland
when put into solution. This can be critical, particularly when
using expensive natural flavors. Other texturizing and stabi-
lizing agents, such as starches, can mask desirable flavors due
to their high usage levels. To overcome this flavor masking,
most developers simply add more flavor (and more cost) to
achieve the flavor impact they desire. What they might not
realize is that by adding more flavors they may actually be
changing what started out as a delicate and refined flavor
profile by overcompensating for the starch in their system.
How to Use Gums
Gums are not starches, and trying to use them the same way
leads to ineffective results and frustration. Gums are easy to
work with once you understand their characteristics.
The first thing to remember is that gums are typically used
at levels far below those of most starches. Most gums are effec-
tive at 0.05 to 0.50 percent, and some are even used at levels as
low as 0.001 percent in certain foods. Starches are often used
at levels in excess of 3.0 percent—often much higher. If you try
to use most gums at that level, the end result is disaster.
Because gums are hydrophilic, they start to swell (hydrate)
the moment they meet water. If too many gum particles are
FIGURE 8.18 Water activity of some common
foods.
Water Activity Typical Foods at That Level
0.99 Fresh meats, poultry, and fish
0.95 Milk, cooked sausage, bread,
cheese spread
0.93 Bean paste
0.92 Caviar
0.91 Swiss and Muenster cheese
0.85 Aged cheddar, fermented sausage
0.82 Semi‐moist foods, pet foods
0.80 Juice concentrates, maple syrup,
fruit cakes
0.75 Jams and marmalades,
marshmallows
0.70 Soy sauce
0.65 Jellies, dried fruits and nuts
0.60 Caramels, toffees
0.50 Dried pasta, spices
0.40 Whole egg powder
0.30 Crackers and cookies
Source: Gum Technology Corporation.
c08.indd 145 1/14/2016 7:22:33 AM
146 • CULINOLOGY®
in contact with one another when they contact water, they
lump or ball up, causing processing difficulties. The key is to
make sure each grain of gum has the opportunity to start
hydrating before it touches another grain of gum. Fortunately,
there are many ways to achieve this.
Remember that gums are not soluble in oil, alcohol, or pro-
pylene glycol, or in high sugar solids solutions. This fact can be
used to advantage in handling the gum. If we add gum to oil with
stirring, it forms a slurry (similar to a slurry of raw starch and
water). As a result, the gum grains each get a coating of oil.
When this slurry is added to water, the grains do not hydrate
immediately; the oil on the grain’s surface inhibits the rapid
exposure to water. This gives the grains time to get away from
each other so when they do hydrate, they remain separated;
therefore they do not stick together or ball up. The same result
can be achieved using alcohol, propylene glycol (PG), or high
solids sugar solutions. Just make sure the slurry is well mixed
right before using so the gum particles don’t fall to the bottom
(exactly like starch in a water slurry). Note: Many of the liquid
flavors used in food manufacturing use alcohol, oil, or PG as a
diluent, so these can be handy mediums for incorporating gums.
Another method of gum dispersion is to pre‐blend the
gum with another granular substance, such as salt, sugar, or
citric acid. When the gums enter the water, each grain is sur-
rounded by a grain of something to which the gum does not
adhere. If we make a blend of 5 parts table sugar to 1 part
gum, each grain of gum is surrounded by 5 grains of sugar,
and when the blend enters the water and the gum starts to
hydrate, it is touching grains of sugar, not other grains of gum.
Types of Gums
The world of gums is filled with diverse products derived from
a wide range of sources. Just as the gums are extracted from
many materials, their functionalities have a broad range as well.
Some gums get viscous, some get very viscous, and some have
little viscosity. Some gums form gels; some do not. Many gums
do not require heat to become functional, but other gums do.
Gels may be soft or firm; some can be melted back into a liquid
by heating (thermo‐reversible) or stay gelled when subjected to
high heat (non‐thermo‐reversible). Some form gels when the
gums are heated (thermogelation); some require the addition of
other ingredients, such as calcium salts or proteins, to form a
gel. Some gums thrive in a low-pH (acidic) environment; others
degrade in that environment. Some gums require high shear to
be effective; others are broken down by high shear.
Some gums, such as xanthan and guar, have long or so‐called
“snotty” textures. Some, such as konjac, have short textures, and
some, such as sodium alginate and some CMCs (carboxymethyl-
cellulose) are in‐between. A long texture forms a thin stream
when poured out of a container; a short texture forms a wider,
less flowing cascade. Starch, after heating, forms a short texture;
corn syrup has a short texture as well. Pancake syrup has a long
texture, as do many mid‐viscosity salad dressings.
Gums can also be synergistic with one another and with
certain other ingredients. Think of synergism as 1 + 1 = 3,
where the sum is more than the total of the parts. If you mix
guar gum that at 0.5 percent yields 500 cps (centipoise) vis-
cosity (see Tech Tip) and blend it with xanthan gum that at
0.5 percent yields a 600 cps viscosity, you would expect the
two together to yield a 550 cps viscosity if each is used at 0.25
percent to create a 0.5 percent solution (250 cps contributed
by the guar and 300 cps contributed by the xanthan). In fact,
the viscosity is higher—about 1400 cps, or about 2.5 times
higher than expected and about 2.8 times the viscosity of the
guar by itself and about 2.3 times the viscosity of the xanthan
by itself. This is synergism at work; the combination is
stronger than either of the two by themselves. (Note: Gum vis-
cosity is not linear at all. The guar used in the above example is
4000 cps at 1 percent but only 500 cps at 0.5 percent. The xan-
than is a bit more linear; at 1 percent it is about 1500 cps, and
at 0.5 percent it is 600 cps.) A heated solution of locust bean
gum flows, as does a heated solution of xanthan gum. But if
you heat the locust bean gum and xanthan gum together, they
form an elastic gel. This is another example of synergism.
The choice of gum is determined by the properties wanted
in the final product, the types of processing conditions the
gum will encounter, and the unique characteristics of the
gum. Talk to your gum supplier before you get started!
Seed Gums
All of the most commonly used seed gums—guar, locust bean
(also known as carob bean), tara, fenugreek, and cassia
gums—are members of the galactomannan family. A galacto-
mannan is a molecule composed of mannose and galactose,
two sugars. The gums and their properties vary due to differ-
ences in the ratio of galactose side chains to mannose back-
bone segments in their molecular structures.
Guar Gum
Guar gum is made from the endosperm of the guar bean.
Guar is grown commercially in many areas of the world,
including Australia and the United States. However, the great
majority of guar gum used in the food industry comes from
India and Pakistan.
Gums
Gum
Sugar
(or other granular material)
FIGURE 8.19 Gum grain surrounded by other
granular material.
Source: Gum Technology Corporation.
c08.indd 146 1/14/2016 7:22:34 AM
CHAPTER 8 • Carbohydrate‐Based Foods • 147
FIGURE 8.20 Considerations in using gums.
Source: Gum Technology Corporation.
Kosher
Halal
Natural
Organic
GMO Free
Price Sensitivity
Process
Hot
Cold
Environment
Neutral
Acid
Final Food Form
Refrigerated
Dry Shelf
Frozen
Equipment Used
High Shear/Low Shear
HTST Processing
Homogenizer/Colloidmill
Other Reactive Ingredients
Salts
Dispersion Helpers
Oil
Alcohol
Sugar
Propylene Glycol
Functionality
Emulsion
Suspension
Gel
Viscosity
Thickening
Parameters
influencing
choice
FIGURE 8.21 Brookfield digital viscometer.
Source: Brookfield Engineering.
Tech Tip
The abbreviation for centipoise is cps, sometimes written cP.
This is a measurement of viscosity or, in simple terms, the
thickness of a fluid. (Viscosity can also be defined as mPas,
or millipascal seconds; 1 cps = 1.0 mPas.) Purified water is
considered 0 cps, tomato juice 100 to 200 cps, creamy
salad dressing 2000 to 5000 cps, yogurt 10,000 to 20,000
cps, corn syrup 45,000 cps, as examples. When we discuss
viscosity, we almost always use a 1 percent aqueous solution
at 77°F (25°C) as the standard. There are exceptions, but,
unless noted, it is safe to assume those parameters.
In the gum industry, viscosity is commonly measured
using Brookfield viscometers, Bostwick consistometers, Bra-
bender consistometers, and visco‐amylographs, although
many other instruments are available. Viscosity is dependent
on temperature and shear (friction between layers) as well as
certain other factors such as time in solution, the amount of
time that shear is applied as well as shear force, and, of course,
the concentration of gum in the solution.
Most Brookfield viscometers measure the amount of
torque needed to keep a spindle moving through a liquid at
a constant speed. The measurement is read in centipoise
(cps). The speed at which the spindle rotates can be con-
trolled to show how the viscosity is, or is not, affected by
changes in shear, which increases as the spindle rotation
speed is increased.
A Bostwick consistometer uses resistance against flow
as a means of measurement. The consistometer measures
the distance in centimeters that a liquid flows down a sloped
trough in a prescribed amount of time (or the amount of
time it takes the liquid to flow to maximum distance).
Brabender visco‐amylographs are most often used to test
starch. They work in a similar manner to the Brookfield; how-
ever, the liquid can be heated and measurements recorded
through a heating and cooling cycle, showing the change in
viscosity with temperature.
These are a few of the more common viscometers used in
the food industry. Of course, each manufacturer makes
models that incorporate any combination of the above
methods.
c08.indd 147 1/14/2016 7:22:40 AM
c08.indd 148 1/14/2016 7:22:40 AM
FIGURE 8.22 Gum attributes.
Product Category
Typical Viscosity
Ranges at 1%
(Temp)
Typical Food
Applications
Typical Use
Level (%) Synergies
Freeze/
Thaw
Stability
Retort
Stability
Stable
pH Range
Water
Solubility
Type of
Emulsifier
Natural or
Synthetic
Agar Marine
gum
5–30 cps
(158°F)
Pie fillings, icings,
and glazes
0.05–0.25 LBG Fair Excellent 2.5–10 Yes
(212°F)
None Natural
Carrageenan
(iota)
Marine
gum
5–400 cps
(167°F)
Custards, dairy
beverages, and
protein beverages
0.02–0.50 Starch Good Good
(pH >4.0)
4.0–10.0 Yes
(>160°F)
None Natural
Carrageenan
(kappa)
Marine
gum
5–400 cps
(167°F)
Water gel desserts,
chocolate
beverages, meats
and meat analogs
0.02–0.75 LBG, Konjac None Fair 4–10 Yes
(>160°F)
None Natural
Carrageenan
(lambda)
Marine
gum
10–1000 cps
(77°F)
Syrups, pizza
sauces, smoothies,
and instant beverages
0.02–0.75 Fair Fair 4–10 Yes
(77°F)
Pseudo‐
emulsifier
Natural
CMC Cellulose
gum
20–20,000
cps (77°F)
Syrups, cake
batters, and instant
drink mixes
0.05–0.75 Locust bean gum,
starch
Excellent Fair 4–10 Yes
(77°F)
None Synthetic
Fenugreek
gum
Seed
gum
1000–3400
cps (77°F)
Salad dressings,
baked goods and
sauces
0.10–0.50 Xanthan Good Fair 2–10 Yes
(77°F)
Pseudo‐
emulsifier
Natural
Gellan Bio‐gum <100 cps
(194°F)
Water gel desserts,
fruit pie filling, and
fruit juice drinks
0.05–0.80 Xanthan Fair Good 1–13 Yes
(≥194°F)
None Natural
Guar Seed
gum
2000–7000
cps (77°F)
Baked goods,
sauces, ice creams,
and popsicles
0.05–0.50 Xanthan, locust
bean gum
Good Poor 4.0–10.5 Yes (77°F) None Natural
Gum arabic Exudates <10 cps
(77°F)
Flavor emulsions,
seasoning adhesions,
and icings
0.50–10.00 Tragacanth Good Good 2–10 Yes
(77°F)
True
emulsifier
Natural
Hydroxypropyl-
methylcellulose
Cellulose
gum
10–100,000
cps (68°F)
Pie fillings, glazes,
and baked goods
0.10–1.00 CMC Good Good 3–10 Yes
(<77°F)
Pseudo‐
emulsifier
Synthetic
Karaya Exudates 200–700 cps
(77°F)
Sauces, cheese
spreads, whole
grain breads
0.05–0.75 Locust bean gum Good None 4–10 Yes (77°F) None Natural
c08.indd 149 1/14/2016 7:22:41 AM
Konjac Plant
gum
20,000–36,000
cps (77°F)
Pasta, meat
analogs, bagels
0.10–1.00 Xanthan,
carrageenan, starch
Good Good 2–10 Yes (77°F) None Natural
Locust bean
gum
Seed
gum
2400–3500
cps(77°F)
Fruit variegates,
cream cheese,
ice cream
0.10–0.50 Xanthan, guar,
carrageenan,
cellulose gum,
agar
Good Good 4–10 Yes
(>140°F)
None Natural
Methylcellulose Cellulose
gum
10–10,000
cps (68°F)
Fried products,
pie fillings, and
meat analogs
0.10 ‐ 1.00 Starch Good Good 2–13 Yes
(<68°F)
Pseudo‐
emulsifier
Synthetic
Microcrystalline
cellulose
Cellulose
gum
<10 cps
(77°F)
Low-fat salad
dressings and dips
0.50–2.00 Cellulose
gum
Good Good 2–10 Yes (77°F) None Natural
Pectin‐HM Pectin 5–200 cps
(77°F)
Jams, jellies,
protein drinks
0.10–0.60 Sodium
alginate
Good Good 2–7 Yes (160°F) None Natural
Pectin‐LM Pectin 5–100 cps
(77°F)
Low-sugar jams
and fillings
0.5 –1.00 Sodium
alginate
Good Good 2–7 Yes (77°F) None Natural
PGA Marine
gum
50–500
cps (77°F)
Salad dressings,
sauces, dips
0.10–1.00 Xanthan Good Fair 3–10 Yes (77°F) True
emulsifier
Synthetic
Sodium
alginate
Marine
gum
10–1000
cps (77°F)
Restructured onion
rings, pimiento filling,
and cheese sauce
0.25–1.00 Xanthan Fair Good in
presence
of calcium
3.5–10 Yes (77°F) None Natural
Tahla Exudates <10 cps
(77°F)
Seasoning adhesions,
added fiber
1.0–20 Good Fair 4.0–10.0 Yes (77°F) None Natural
Tara Seed
gum
3000–7000
cps (77°F)
Ice cream,
cheesecake, and
sauces
0.10– 0.50 Xanthan,
carrageenan
Good Good 2–10 Yes (77°F) None Natural
Tragacanth Exudates 200–4600 cps
(77°F)
Salad dressings,
icings, and flavor
emulsions
0.05–0.40 Gum arabic Good Good 2–10 Yes (77°F) True
emulsifier
Natural
Xanthan Bio‐gum 1100–1700
cps (77°F)
Salad dressings,
baked goods,
and sauces
0.05–0.40 Guar, locust bean
gum, konjac, CMC,
microcrystalline
cellulose, starch,
gellan
Excellent Good 1–12 Yes (77°F) Pseudo‐
emulsifier
Natural
Source: Gum Technology Corporation.
150 • CULINOLOGY®
Guar is one of the more familiar gums to food scientists
and research chefs. One reason is that it is historically
inexpensive, usually the least costly of all the major gums,
and fairly viscous. If viscosity is your main concern, guar gum
gives you a good bang for the buck.
Typical guars have viscosities in the 4500 to 7000 cps range
(the creamy salad dressing range, or slightly higher), depending
on the quality of the bean and how it is processed. Guar gum
can be purchased in many viscosity ranges and in many mesh
sizes. Mesh size of guar and other gums is a measurement of
how fine or coarse the powder is. Mesh is usually referenced in
particles per square inch; a larger number (200 mesh as
opposed to 40, for instance) means a finer powder. The mesh
size is determined by sifting through standard screens (sieves).
Coarser meshes are more easily dispersed in water; there is less
chance of lumping or balling up. On the other hand, finer
meshes hydrate and create viscosity more rapidly.
Guar hydrates and gets viscous in cold water; it does not
require heat to become effective. This is a great energy savings
and very useful for instant powdered beverages and other
items where quick, inexpensive viscosity is desired. It also
maintains moisture in baked goods, especially sweet baked
goods. Although guar eventually gives up some of its water to
the environment, it holds on long enough to be an effective
shelf life extender in baked goods. One disadvantage is its
slightly beany odor. In baked goods and at low usage levels,
the odor or taste is not apparent. Guar can also be slimy or
snotty in liquid applications if used at too high a level.
Guar is synergistic with xanthan gum—that is, the two
together form a more viscous solution than expected based
on their individual thickening abilities. Choosing whether to
use the gums as a single entity or in combination depends on
a number of factors including, but not limited to, cost, final
texture desired, simplification of process, purchasing, and
labeling. To simplify processing, the gums can often be pur-
chased as blended stabilizing systems; however, the label dec-
laration must still list each gum used in the blend separately.
In the following recipe, rice flour and potato starch replace
wheat flour to make the recipe gluten free. Guar gum and
xanthan gum are used to replace the elasticity and structure
the gluten found in wheat flour normally provides.
Gluten‐Free Chocolate Brownies
Ingredients Percent
White rice flour 7.00
Cocoa powder 6.00
Potato starch 3.50
Guar gum 0.30
Xanthan gum 0.45
Vanilla extract 0.75
Sugar 36.75
Vegetable oil 19.00
Eggs 17.50
Chocolate chips 8.75
Total 100.00
Procedure:
1) Sift together the white rice flour, potato starch, cocoa
powder, and gums. Set aside.
2) Whisk together the vegetable oil, eggs, sugar, and vanilla.
3) Combine the wet and the dry ingredients. Stir in chocolate
chips.
4) Pour into greased pans and bake at 325°F for 25 to
30 minutes, or until set in center.
5) Allow to cool completely before cutting.
Locust Bean Gum
Locust bean gum (LBG) comes from the seed of the carob pod,
from the carob tree (Ceratonia siliqua); it is also known as carob
bean gum. The carob pod is also called St. John’s bread and has
been used as a food ingredient in the Mediterranean for centu-
ries and, more recently, as a chocolate substitute. LBG is grown
in Portugal, Morocco, Spain, and Italy. To get the gum, the seed
is split open and then mechanically ground and sieved to sepa-
rate the endosperm from the germ and the husk.
Locust bean gum is only slightly soluble in cold water and
must be heated to at least 140°F (60°C) in order to start swell-
ing and reach full hydration (heating to 185°F [82°C] is rec-
ommended). When LBG is heated and subsequently cooled,
it forms a smooth, flowing solution with a shorter texture
than most other galactomannans; it has been described as a
FIGURE 8.23 Galactomannan ratios. Fenugreek
gum has one galactose side chain for every
mannose molecule on the backbone, whereas guar
gum has one galactose for every two mannose
molecules, tara gum has a 1:3 ratio, and so on.
Source: Gum Technology Corporation.
Germ
Endosperm
Grain
Pod
Guar Hull
FIGURE 8.24 Guar seed, expanded illustration.
Source: Gum Technology Corporation.
c08.indd 150 1/14/2016 7:22:41 AM
CHAPTER 8 • Carbohydrate‐Based Foods • 151
pseudo‐gel. At 1 percent, after heating and cooling, LBG has a
typical viscosity of about 2500 cps, although, as with most
gums, this can vary.
LBG is synergistic with other gums, including carra-
geenan, xanthan, and agar. A heated 1 percent solution of
xanthan gum is pourable and a heated 1 percent solution of
LBG is pourable, but if you combine them before heating
(0.50 percent each for a total of 1 percent), the result is a very
elastic gel after heating and cooling—a gummy candy type of
texture. LBG is also helpful in controlling syneresis and, as
such, is often used in dairy products such as cream cheese
and puddings to help prevent and control excess water from
weeping out of the product. Locust bean gum is often used in
gelatin‐free vegetarian dessert gels created with kappa
carrageenan or agar, as both of these gums form gels that
have a relatively high degree of syneresis. LBG also has the
effect of softening gels—that is, making them less brittle and
more elastic. This is useful in products such as flans and
crème brûlée. Many ice cream stabilizers also use LBG for its
ability to hold water and prevent ice crystal formation, mak-
ing frozen desserts feel smoother and creamier.
Locust bean gum also finds application in fruit variegates—
the fruit‐and‐sugar preparations mixed into ice creams,
yogurts and pie fillings. Its short texture looks like starch but,
unlike starch, LBG does not mask flavors.
Tara Gum
Tara gum is closely related to locust bean gum and guar gum.
It is derived from the endosperm of the seed of the tara tree
(Cesalpinia spinosa lin), also known as Peruvian carob. It is
harvested and processed much like LBG gum.
Tara has a 1:3 ratio of galactose to mannose. Guar gum has
a 1:2 ratio, and locust bean gum has a 1:4 ratio. It is not sur-
prising, then, that tara gum has properties somewhat like
LBG and somewhat like guar gum. Tara is partially soluble in
cold water (guar is totally soluble in cold water) but com-
pletely solubilizes in hot water, as does locust bean gum
(which does not dissolve in cold water).
The viscosity of a cold‐water solution of tara falls between
that of guar and locust bean gums, and pricing historically is
also in the middle. After heating, tara gains viscosity. Thus,
tara makes a good substitute for LBG and a replacement for
guar if guar prices rise or supplies become limited.
One of the more common uses for tara gum is in combina-
tion with xanthan to create the soft gels commonly found on
top of canned pet food. This was traditionally made with
locust bean gum and xanthan; however, the lower cost of tara
and the unimportance of clarity in this product make tara
gum a good option.
Tara gum has not achieved the popularity of guar gum and
locust bean gum in part because of its somewhat limited
availability in the past. However, this has changed in recent
years as more growers and processors, particularly in Peru,
have seen it as an economically feasible product; production
and availability have increased as a result.
Fenugreek Gum
Fenugreek gum is a relative newcomer to the world of com-
mercial hydrocolloids. Fenugreek is an herb or spice that is
commonly used in Indian curries as well as in many African
and Mediterranean cuisines. In addition, sotolone, a potent
aroma chemical extracted from fenugreek, is commonly used
to flavor imitation maple syrup. As with all the other seed
gums, the gum portion of fenugreek comes from the
endosperm of the seed.
Fenugreek gum has a viscosity similar to that of xanthan
gum (about 1500 cps at 1 percent in water) and a smooth,
flowing texture. It can have a slight maple odor; however,
new advances in production yield powders with almost no
odor.
Besides thickening and texturizing, fenugreek also exhib-
its exceptional nutraceutical qualities, such as the reduction
of blood cholesterol and a lowering of the glycemic index of a
food. Although usually not enough gum is used in any one
product to substantiate a claim, it is useful as a part of a total
functional food system to be able to claim that the texturant
used has functional benefits.15
Tree Exudates
Tree exudate is another term for sap. Tree exudates were
probably the first form of gums to be used for foods, as they
are easy to harvest and process. Maple syrup is condensed
sap from the maple tree. The primary difference between
maple sap and gum exudates is that the latter, oozing from
the tree, harden as they comes into contact with oxygen and
the ambient environment.
Acacia (Gum Arabic)
The term gum arabic is used with varying degrees of preci-
sion. In the context of food additives, the most recent inter-
national specification published by the Food and Agriculture
Organization (FAO) defines gum arabic as the “dried exuda-
tion obtained from the stems and branches of Acacia senegal
(L) Willdenow or closely related species.”16 In Sudan, the term
gum arabic refers to two types of gum that are clearly sepa-
rated in both national statistics and trade: hashab (from
A. senegal) and talha (from A. seyal). In a still wider sense, gum
Germ
Endosperm
Grain
Pod
Carob Hull
FIGURE 8.25 Locust bean pod, expanded.
Source: Gum Technology Corporation.
c08.indd 151 1/14/2016 7:22:42 AM
152 • CULINOLOGY®
arabic is often taken to mean the gum from any acacia species
(and is sometimes referred to as acacia gum).17
The above quote explains the frequent confusion in the
use of the term. Only the A. senegal form can be used as an
emulsifier, and we refer to that variety only as gum arabic.
Gum arabic has a long history of use in the food industry
as well as in many non‐food applications, including as an
adhesive on envelopes and stamps, as a thickener in inks,
and for sizing on cloth. There are many reasons why gum
arabic has been used for such a long time and why it contin-
ues to be so popular. First is that it becomes sticky when
wet. This is obvious even as it is hanging as a teardrop on
the tree; touching the sap before it dries makes your fingers
feel sticky.
The second is that it can be made usable simply by adding
it to water; even a large piece of gum arabic dissolves in water
relatively quickly. It is the only gum that is truly soluble in
cold water. Processing the gum is easy; it is mechanically
ground into a powder for faster dispersion and hydration.
As previously stated, gum Arabic senegal is the true emul-
sifier; its lipophilic (fat‐loving) and hydrophilic (water‐ loving)
receptors are found in its arabinogalactan protein (AGP). For
emulsifying properties, use this species.
One of the more interesting aspects of gum arabic is its
low viscosity. For the most part, gums are thickening agents.
Gum arabic exhibits very low viscosity when put into solu-
tion. A 30 percent gum arabic solution is only about 1200
cps18; compare that to a 1 percent solution of xanthan, which
is about 1500 cps. In fact, it is possible to put gum arabic into
solution as high as 55 percent. This makes it useful for a num-
ber of applications, one of which is spray‐drying.
To spray‐dry a flavor, for example, first make a solution
from a soluble solid such as gum arabic and add some type of
liquid, oil‐based extract, or flavorant to that solution. To turn
this into a dry flavor, the liquid must be removed. One way to
do that is spray‐drying, which consists of passing the flavor
solution through a very fine spray nozzle (like a misting sys-
tem) into a chamber with circulating heated air, hot enough
to instantly evaporate the water. Usually spray‐dryers are ver-
tical and the product is sprayed into the chamber at the top.
By the time it reaches the bottom, the hot air has evaporated
all of the liquid, leaving behind only the solids (in this case
gum arabic) on which the flavorant is adsorbed (coated).
Because gum arabic can be put into solution as high as 55
percent and still be thin enough to pass through the spray‐
dryer’s nozzles, 55 pounds of powdered flavor can be pro-
duced for every 100 pounds of liquid sprayed. If guar gum
were used instead, it would probably be too viscous to spray,
even at just a 0.5 percent solution, meaning that every 100
pounds of solution would yield less than half a pound of dry
powder. In addition, even at high use levels, gum arabic has
no off‐taste to mask the flavor being spray‐dried.
In addition, many flavors are oils or use oil carriers. Gum
arabic is a true emulsifier and therefore holds onto the flavor
oils better than many other solids, such as sugars and
dextrins. This is one reason why gum Acacia senegal is used
for so many flavor emulsions, especially in beverages such as
colas that use oils as a flavor base. It is also useful in dressings
and sauces where emulsification is required, but not much
added viscosity.
Gum arabic is also used in candies to prevent sugar crystal-
lization19 and in confections as a coating. It is used as a glaze
on baked goods and to stabilize foams, beers, and lagers.20
Gum arabic was for many years used as an adhesive for
envelopes and stamps, in part because it can be made into a
high-concentration solution and in part because of its adhe-
sive qualities. Gum arabic glue was produced by making a
solution with a high concentration of gum arabic. As it dried,
the solution left a film of gum arabic—which, when rewetted,
became sticky, and as it dried again, adhered to whatever it
touched. More efficient adhesives are used today, but gum
arabic is still useful in making flavored edible films, such as
breath strips.
Gum arabic is also an excellent choice for incorporating
into high‐fiber foods. Because it is about 85 percent soluble
fiber on a dry weight basis (DWB) (the weight of the material
if all water were eliminated)21 and because it has little impact
on viscosity or texture, it is an excellent way to add fiber to a
beverage, baked good, or nutritional bar.
In the following recipe, just a small amount of gum arabic
helps create a true emulsion; the xanthan and lambda car-
rageenan impart body and help create a pseudo‐emulsion.
Honey Mustard Vinaigrette
Ingredients Percent
Vegetable oil 55.15
White vinegar (5 percent acidity) 31.10
Honey 5.50
Dijon mustard 3.95
Salt 3.15
Garlic powder 0.40
Powdered gum arabic 0.15
Xanthan gum 0.15
Lambda carrageenan 0.15
Black pepper 0.25
Hot sauce 0.05
Total 100.00
Procedure:
1) Blend all dry ingredients.
2) Add dry mix slowly to one‐quarter of the oil using high
shear—that is, in a high‐speed lab mixer.
3) Mix for 5 minutes to assure maximum viscosity.
4) Mix hot sauce, Dijon mustard, white vinegar, and honey
together.
5) Add honey mixture to gum/spice/oil mixture and continue
mixing for 5 more minutes.
6) Add the remaining oil and mix for 2 minutes.
7) Pour into a container and shake for 30 seconds.
8) Over the next few days, watch the dressing for signs of
separation or thinning.
c08.indd 152 1/14/2016 7:22:42 AM
CHAPTER 8 • Carbohydrate‐Based Foods • 153
Talha (Acacia Seyal )
The other popular form of gum arabic is A. seyal, frequently
called gum talha.
Essentially, gum talha can be used in the same applica-
tions as gum arabic, with the one very important exclusion of
emulsions. Gum talha does not help stabilize an emulsion. It
is a good choice for creating films, adding bulk, and adding
fiber, just as gum arabic is. Gum talha is generally less expen-
sive than gum arabic and, because it grows in a far wider
region than gum arabic, more available. So unless emulsifica-
tion is needed, gum talha is a better choice.
Tragacanth
Gum tragacanth (often called trag or gum trag) is the dried
exudate of the shrub Astragalus (Leguminosae), including
the species A. adscendens, A. gummifer, A. tragacanthus, and
A. microcephalus. Gum tragacanth is grown in the Middle
East, with the majority coming from Iran and Turkey.
Gum tragacanth sap grows as ribbons or flakes after the
bark and taproots of the plant are scored with hatchets and
knives.
Gum tragacanth is hand‐picked and sorted into grades,
the highest grade (ribbon #1) being white and free of extrane-
ous materials such as sand and bark. Because the sap starts
as a liquid, the ribbon or flake can pick up blown sand,
insects, and other foreign matter as it is drying. The ribbons
with the most sediment are graded #5. Some tragacanth exu-
dates dry as flakes and are generally graded lower than
ribbons.
Unfortunately, gum tragacanth is not widely available.
Harvesting and sorting is labor‐intensive and expensive, and
the “interventions by the Iranian government effected a
sharp rise in export price”22 during the 1980s. The introduc-
tion of xanthan gum also diminished the demand for gum
tragacanth, and so it is not widely used today. The price is
currently between 4 and 15 times higher than that of xan-
than, depending on grade.
Gum tragacanth is acid‐resistant and, like A. senegal, a
true emulsifier. This makes the gum ideal for use in salad
dressings and some sauces. In fact, prior to the introduction
of xanthan gum, gum tragacanth was probably the most
widely used gum for this purpose. Today, one of the most
common uses of gum tragacanth is in sugarcraft, the art of
making sugar pastes into decorations, usually for bakery
items. The tragacanth forms an easily workable and pliable
material when mixed with sugar and water, and it retains its
consistency long enough, without drying and cracking, to
remain fresh‐looking on the cake. Another common use is as
the adhesive holding the outer leaf wrapper around a cigar
filler. Both of these exploit the adhesive properties of traga-
canth as a paste at 2 to 5 percent. Because of its excellent
resistance to acid conditions, gum tragacanth is also used in
relishes and some pickling solutions. Again, its cost and rela-
tively limited production hinder its wider use in the food
industry.
Karaya
Karaya, also known as sterculia gum, is the dried exudate of
Sterculia urens trees.23 Gum karaya does not fully dissolve in
water; the granules swell to form a viscous solution. Karaya is
used in food products such as barbecue sauces and spice
rubs and was, until the development of synthetic polymers,
used as a denture adhesive. It is still used in conjunction with
glycerin as an adhesive for colostomy bag sealing rings.
Karaya passes through the body without being digested, so it
is also used as a laxative.
Most karaya comes from India; other sources include
Senegal, Mali, and Pakistan. Like gum arabic and gum traga-
canth, the karaya tears are collected by hand from cuts made
in the bark of the tree, and it is sorted, cleaned, and mechani-
cally processed into a powder or granular form.
Karaya, at higher pH levels, exhibits a ropy texture. For
example, after hydration, raising the pH above 6.0 increases
viscosity, and the solution develops ropiness. This can be useful
in products where dripping must be controlled—for example,
in a sauce placed in a pump dispenser in a foodservice set-
ting. Karaya reduces the amount of drip as the ropiness pulls
the sauce back up into the dispenser.
Gum karaya can also be used to prevent bleed‐out of color
in frozen desserts, including sherbet and ice pops.24
Bio‐Gums
Bio‐gum is the term for gums produced through fermenta-
tion or that start with living components. A number of bio‐
gums are produced, including xanthan, gellan, dextran,
pullulan, and curdlan; however, the only two currently used
with any regularity in the food business are xanthan and gel-
lan, and the use of gellan gum is minor at this point.
Xanthan
Xanthan gum was developed by the USDA in 1959 and
licensed to the Kelco division of Merck and Company, which
produced it commercially starting in 1961. It was approved
for food use in 196925 and, since then, has become one of the
most widely used gums in the food industry.
Xanthan gum is produced through the growth on sugars
of the Xanthomonas campestris bacterium, which is often
found on cabbage. Xanthan gum is the extracellular polysac-
charide produced by the bacteria during fermentation. After
the bacteria are pasteurized to stop fermentation, the poly-
saccharide is precipitated by alcohol. The result is dried and
ground into a powder. Xanthan gum is considered a natural
gum.26
Xanthan is a popular choice in many foods. It is acid‐
stable, temperature‐stable, relatively unaffected by salts,27 and
synergistic with galactomannans (the seed gums). Xanthan
gum is also a good choice in frozen foods; it is fairly freeze‐
thaw resistant. On its own, it is non‐gelling, but in combina-
tion with locust bean gum forms a soft, elastic gel. Xanthan
gum is pseudoplastic, meaning that at high shear it thins out,
c08.indd 153 1/14/2016 7:22:42 AM
154 • CULINOLOGY®
but that at low or no shear it returns to its thickened state.
This property makes it useful in products such as salad dress-
ings; when poured, the shear created by the pour makes the
dressing thin out; however, when it comes to rest on the salad
and there is no longer any shear, it thickens and clings.
Xanthan gum is relatively inexpensive and, because it is
manufactured rather than harvested, not subject to environ-
mental or geopolitical considerations. Some other gums
become less available due to droughts, plant disease, political
embargoes, and other factors that can affect distribution.
Because xanthan is not subject to these, its availability and
pricing remain stable from year to year.
One problem is that its texture in solution can be snotty.
This is addressed by adding other gums, such as guar. Also,
this negative attribute of xanthan does not manifest in dry
products, such as baked goods.
Xanthan is generally sold in two mesh sizes, 80 and 200.
The 200 mesh size hydrates more quickly but is a bit more dif-
ficult to disperse. The 80 mesh is easier to disperse but
hydrates less quickly. Other available forms are agglomerated
(individual particles stuck together into large, irregularly
shaped clumps that rapidly disperse in water and are less
dusty and easier to handle than standard powders) and easily
dispersing (usually coated with a small amount of oil), as well
as transparent and brine‐tolerant grades. An agglomerated or
easily dispersible xanthan may be best if the equipment used
does not produce much shear or water movement, as some
swept‐surface kettles. Brine‐tolerant xanthan is an excellent
choice for products high in salt, such as pickle brines and
some marinades.
Xanthan gum is widely used in salad dressings to form a
pseudo‐ or commercially acceptable emulsion. In fact, many
gums possess this emulsion‐like property.
A true emulsifier contains a lipophilic (attracted to oil)
and hydrophilic (attracted to water) receptor. Gum arabic
and gum tragacanth have molecules with these receptors.
By holding onto both the water and the oil, they create an
emulsion where the water and the oil do not separate; they
are held together by a common molecule to which they
are both bound. A commercially acceptable or pseudo‐
emulsion is different; it looks like an emulsion, but it is not
really emulsified.
An emulsion has two parts: an internal, dispersed phase
and an external, continuous phase. In a soda pop illustration,
the bubbles are the internal phase and the surrounding water
is the external phase. Of course, when the bottle or can is
opened, the small bubbles (the internal phase) rise to the top
because they are lighter than the external phase. The same
happens when you mix oil and water; when you shake the
mixture, the oil slick breaks into little globules or droplets and
becomes dispersed in the external phase. However, because
oil is lighter than water, the oil droplets rise and aggregate
(come together and form larger globules) and eventually coa-
lesce (form a solid, unified layer of oil). Now, think of a thick
shake; there are still small bubbles (internal phase) dispersed
in the shake, but they are not moving. Why? Because the
external phase, the semi‐frozen solution around them, is too
thick. Eventually, as the shake melts and thins, the bubbles
rise; for the moment, though, they are distributed throughout
the external phase.
That is essentially how a pseudo‐emulsion or commer-
cially acceptable emulsion works. For example, a salad dress-
ing containing oil is passed through a homogenizer or colloid
mill that makes the oil drops very, very small (less than 10
microns). Xanthan gum makes the external phase, or water‐
vinegar solution, thick. This makes it hard for the very small
oil droplets to move and rise to the top—a commercially
acceptable emulsion. It is called commercially accept able
because it is not a true emulsion; there is no emulsifier to
hold the two components together, and it will eventually
break (separate into oil and water phases again), but it will
remain as an emulsion long enough to be sold and consumed
by the end user.
As mentioned, xanthan is probably the most widely used
of all gums due to its effectiveness across a wide range of cri-
teria. However, while it can be considered a Swiss Army knife
that performs many functions adequately, it may not offer
optimal performance in every case. That is one reason why
xanthan is often used in conjunction with other gums. Many
salad dressings in the market contain xanthan gum and
Propylene Glycol Alginate (PGA). This combination, typically
90:10 xanthan:PGA, is one of the most common stabilizer
systems used in the food industry.
The following salsa recipe contains xanthan and guar gum,
a good combination due to their synergism. The combination
allows for reduced costs and lower overall use of gum, result-
ing in a nice, smooth texture. The use of two gums that are
efficacious in a cold system makes possible a non‐cooked
salsa.
Salsa
Ingredients Percent
Tomatoes, canned diced 71.85
Red onion, diced 13.10
Green pepper, diced 11.05
Vegetable oil 1.30
Vinegar, white 5 percent acidity 1.30
Salt 0.55
Garlic powder 0.30
Guar gum 0.10
Xanthan gum 0.15
Ground cumin 0.20
Black pepper 0.10
Total 100.00
Procedure:
1) Dry blend the salt, garlic powder, gums, cumin, and black
pepper.
2) Puree or coarsely chop (depending on desired con-
sistency) the tomatoes, onions, and green peppers. Com-
bine with the oil and vinegar.
c08.indd 154 1/14/2016 7:22:42 AM
CHAPTER 8 • Carbohydrate‐Based Foods • 155
3) Add the dry ingredients to the tomato mixture and blend
until fully dissolved.
4) Look for signs of separation over the next few days.
Gellan Gum
Gellan gum, discovered in 1977,28 is manufactured via fer-
mentation similar to xanthan gum. The bacteria used is
Sphingomonas elodea, which has been considered, in Japan, a
natural food additive since 1988 and approved by the FDA for
use in foods (21 CFR 172.665).
Gellan gum can form weak gels at low usage levels, excel-
lent for the suspension of particulates, such as fruit particles
and pulp, in beverages including ades, smoothies, and iced
teas.
Gellan gum is available as high acyl or low acyl. High‐acyl
gellan gum yields a soft, elastic gel, while low‐acyl gellan gum
forms hard, brittle gels in the presence of cations (molecules
with a positive charge—that is, with more protons than elec-
trons), including calcium, magnesium, sodium, and potas-
sium.29 This makes gellan gum an excellent choice for creating
gels because, with the right choice (high acyl or low acyl) and
by varying the amount and type of cationic salt (such as
potassium chloride or magnesium chloride) used, a wide
range of gel textures is possible.
Another unique property of gellan is its ability to control
its setting point (the temperature at which it goes from liquid
to gel) and its melting point. These factors are controlled,
again, by the choice of cation; gels made with calcium set
between 77 and 113°F (25 and 45°C), while those made with
sodium set between 104 and 122°F (40 and 50°C). At lower
levels of cationic salts, gels are thermo‐reversible—that is,
they remelt when heated; at higher ion levels, they are non‐
thermo‐reversible—that is, they do not remelt when heated.30
Non‐thermo‐reversible products are desired when a prod-
uct’s texture should remain firm after heating, as in an analog
(soy or vegetable) sausage. The gum creates a gel that gives
the product structure; melting is not desirable. On the other
hand, a thermo‐reversible product is perfect for coating a tur-
key with a glaze that adheres to the surface but that, when
heated, melts to form a gravy or jus.
Gellan gum can substitute for agar or gelatin in dessert
gels; it can form a softer gel than agar can.
Because it is not derived from animals, as gelatin is, gellan
can be used in vegan and meat‐restricted diets. It is suitable
in kosher and halal foods for the same reason.
Gellan is relatively expensive compared to other gums.
However, it is growing in popularity and, with increased sales
volumes, prices are likely to drop.
Marine Gums
Marine gums come from the oceans; more specifically, they
are derived from seaweeds.
There are many types of marine gums, and within each
category are many variations. This is primarily due to the
wide assortment of seaweeds and their individual properties
but also because of the choice of processing methods and
chemical reagents used in their manufacture.
This makes it difficult to place marine gums in any one
category—some form gels, some do not; some require heat to
gel, others do not; and so on. However, the extremely wide
range of attributes is also what makes marine gums so useful;
no matter what type of system is in development, chances are
that a marine gum will work.
In fact, variables are so numerous they would fill an entire
book. Here we list the most common properties and uses. As
an example, the presence of calcium, sodium, and potassium
ions affects hydration temperatures and, therefore, the set-
ting and remelting temperatures of the carrageenan gels. Iota
carrageenan hydrates at ambient temperature in water and
becomes a viscous solution. However, if salt is added, the
solution is converted into a slight gel with a distinct yield
point (essentially a measure of resistance to flow ; true solu-
tions do not have a distinct yield point). The new yield point
makes the gum useful in products such as cold prepared
salad dressings, as the slight gel suspends particulates, such
as herbs, while retaining a pleasantly fluid mouthfeel.31 This
also helps the dressing cling to the salad vegetables rather
than quickly drain to the bottom of the bowl. The most com-
monly used parameters presented here are safe and yield an
effective product.
Carrageenans
Carrageenans come from a class of seaweed called
Rhodophyceae, or red algae. Within this class of seaweeds are
the Chondrus crispus, Euchema cotonnii, Euchema spinosum,
Gigartina skottsbergi, and Iradaea laminarioides species.32
Carrageenans are classified by their primary molecular
structure as either kappa (κ), iota (ι), or lambda (λ). Each type
acts differently; however, it is very rare for a carrageenan to
be pure iota or lambda or kappa. In fact, each seaweed usu-
ally contains two of the types. Chondrus crispus, which is also
known and sold in health food stores as Irish moss, contains
kappa and lambda carrageenans. The Euchema species con-
tains kappa and iota carrageenans, and the Gigartina species
yields kappa and lambda carrageenans.33 When we say a car-
rageenan is a kappa carrageenan, we mean it is predomi-
nantly kappa.
The seaweeds are either harvested from the ocean or
farmed. Farming is becoming preferable because it can be
closely controlled. In the Philippines, the government gives
families starter stock that they plant along the coast, under
water, about waist high in a manner similar to a vineyard,
with lines strung between stakes planted in the sand and the
seaweeds suspended from the lines.
The weeds are picked when they reach a predetermined
length, and are cleaned and then placed in extraction
tanks. They are processed with various alkalis (potassium
hydroxide, sodium hydroxide, and so on) to break down the
cellulose. The mixture is clarified by passing through filters,
c08.indd 155 1/14/2016 7:22:42 AM
156 • CULINOLOGY®
concentrated, precipitated (usually with alcohol), and then
dried and ground. Because there are so many seaweeds and
combinations of variables, from the ratio of kappa to iota to
lambda, to the choice of alkalizing agent, to the choice of
precipitating agent, to the manner in which the product is
dried and ground, to the naturally occurring mineral salts,
several hundred, if not thousands, of variations of carra-
geenan are possible. Think of choosing a carrageenan like
you would choose paint. First, is it latex, acrylic, or water‐
based? Second, is it matte, gloss, or semi‐gloss? Third, what
color do you want? Is it blue? If so, robin’s‐egg blue, sky blue,
navy blue? Just as paints can be as different as a glossy
Chinese red is from a matte beige, and just as they can be as
close as two adjacent color chips, carrageenans can be that
different and that similar. They provide the food developer a
very wide range of tools for use in a very wide range of
conditions.
Kappa (κ) Carrageenan
Kappa carrageenans gel on heating to at least 176°F (80°C)
and cooling. In water, they form a clear gel that, depending on
the concentration of carrageenan, can be soft or brittle. As
with gellan gum, the presence of ions (such as sodium and
calcium) alters the setting and melting temperature within a
range of 104 to 158°F (40 to 70°C). These gels are thermo‐
reversible when heated to 41 to 50°F (5 to 10°C) above their
gelling temperature. Kappa carrageenans react nicely with
potassium ions, forming stronger gels when potassium is
present in the system.
Kappa carrageenans are not very freeze‐thaw stable, and
they exhibit a fair amount of syneresis. However, the addition
of other gums, especially locust bean, inhibits the syneresis
and yields a softer, more gelatin‐like gel. When used in this
manner, kappa carrageenans are used to make a dessert gel
that is shelf‐stable when filled and packed aseptically (in a
sterile environment in sterilized packaging materials).
Because the melting point of the carrageenan is around 140°F
(60°C), it is unlikely to melt when stored in a non‐refrigerated
state.
Kappa carrageenans also react with milk proteins. They
not only form a weak gel in the water portion of milk but also
interact with the casein micelles (typical casein structures)
that make up 80 percent of the milk proteins, and they act as
an emulsifier due to being both hydrophilic and lipophilic.34
This makes kappa carrageenans an ideal choice for products
such as flans and crème brûlée. Because the carrageenan
incorporates the casein micelles of milk into its gel structure,
the result is about 5 times as firm as a water gel.35 In other
words, a milk gel made with 0.2 percent carrageenan is as
firm as a water gel at 1.0 percent carrageenan. The econo-
mies of use make kappa carrageenan an especially good
choice in products containing milk components.
One of the more popular uses is as a water binder in meat
products. The meat processor injects a solution of water and
kappa carrageenan into the meat muscle, thereby adding
moisture and weight. The carrageenan helps prevent purge,
or water loss, by forming a gel inside of the protein that effec-
tively traps the added water.
Iota Carrageenan
Iota carrageenan tends to form soft, elastic gels. It is very
reactive with calcium, making it another excellent choice for
foods containing milk or cheese. Iota carrageenan exhibits
better freeze‐thaw stability than kappa. Like kappa, heating is
an effective mechanism for creating a gel, although iota car-
rageenan also forms a gel in cold milk because of its interac-
tion with calcium. With a very small amount of carrageenan
(0.01 to 0.05 percent), a very weak gel structure forms that
acts as a web in the solution, trapping particles that would
otherwise settle. This makes iota (and also kappa) carrageen-
ans excellent choices for beverages such as chocolate milk
and bottled cappuccinos, where the cocoa particles would
otherwise settle to the bottom of the container.
Iota carrageenans are often used in conjunction with
kappa carrageenans. The combination offers a wide range of
gel structures; adding more iota makes the gel less brittle and
softer, while adding more kappa makes it a bit stronger and
more firm. As the iota does not exhibit as much syneresis as
the kappa, watering out is reduced when iota is added to a
kappa gel. Freeze‐thaw stability in kappa is improved by
adding iota, and as both are reactive with milk products, com-
bining them yields a wide array of gels—from firm custard to
soft pudding and even pourable yogurt. Iota carrageenans are
also synergistic with starch; a starch solution increases in vis-
cosity approximately four times when iota is introduced.36
This combination is often used to give texture to bakery
cream fillings.
In the following recipe, the iota carrageenan helps keep
the sauce consistent by creating a slight gel matrix with the
cream and butter. The xanthan adds body, and the low‐
methoxyl pectin helps prevent protein denaturation (cur-
dling) on reheat.
Make two batches, one containing the gums, the second
without as a control. Then do the comparison tests recom-
mended below.
Alfredo Sauce
Ingredients Percent
Heavy cream 63.50
Parmesan cheese, grated 22.70
Black pepper 0.18
Salt 0.36
Granulated garlic 0.27
Iota carrageenan 0.15
Low‐methoxyl pectin 0.07
Xanthan 0.07
Plugra butter 12.70
Total 100.00
c08.indd 156 1/14/2016 7:22:42 AM
CHAPTER 8 • Carbohydrate‐Based Foods • 157
Procedure:
1) Scale the dry ingredients; mix together thoroughly to
ensure even distribution of all the ingredients.
2) Place the heavy cream in a saucepan and begin to heat.
While heating, rapidly whisk in the dry ingredients until
evenly blended and well incorporated.
3) Once the cream mixture has come to 180°F (82°C),
whisk in the cheese until it melts and becomes fully
dissolved and blended with the cream.
4) Bring to a simmer and continue simmering for 5 minutes.
While doing so, break the butter into small pieces and
whisk it in, a few pieces at a time, until the butter is
melted in and evenly blended.
5) Remove from the heat. Chill.
What you should notice:
• The cook time for the gum sample is 5 minutes.
• The control takes 15 minutes to reach final viscosity,
which is thinner than the gum sample.
• The gum sample requires 66 percent less heating time
than the control to achieve full viscosity.
• The yield for the gum sample is 471 g (85.5 percent
yield); the control with no gums yields 400 g (72.5
percent) due to evaporation of the liquids in order to
achieve viscosity.
Test the two sauces by adding 200 g sauce to 300 g cooked
linguine. Freeze the samples and reheat in a microwave after
freezing. Notice that the sample with gums has a better
consistency—but, more important, the emulsion remains
intact, with very little separation of fat.
Lambda Carrageenans
Unlike kappa and iota carrageenans, lambda carrageenans
do not gel, but they do become viscous when dispersed in an
aqueous solution. Lambda does not require heating to
become effective; it disperses and hydrates in cool water.
Lambda carrageenans exhibit good salt tolerance. This
makes them a good choice for salad dressings and light
brines where suspension of spices is desired. Lambda can be
a good substitute for other thickening gums, such as xan-
than and guar, especially when a less snotty or gummy
mouthfeel is desired. Lambda has a clean mouthfeel and is
smooth‐flowing. The predominant use for lambda carra-
geenan in the food industry is as a thickener for dairy prod-
ucts, adding texture, mouthfeel, and suspension properties.
It is also used in products such as retail dry mix salad
dressings.
Alginates
The alginate used in the food industry comes from brown
algae (Phaeophyceae), of which there are many varieties,
including Laminaria hyperborea (perhaps the most com-
mon), Macrocystis pyrifera, and Laminaria japonica.
The most common form is sodium alginate, so much
so that when people in the food industry talk about sodium
alginate they usually just say “alginate.” Other forms exist
(potassium alginate, calcium alginate), but the sodium form
predominates.
Alginate is extracted from seaweed by first making a pre‐
extraction with hydrochloric acid. This yields alginic acid,
which has limited stability37 and so is quickly processed with
sodium carbonate or sodium hydroxide to yield sodium algi-
nate. The actual process is far more complex.
Alginate is composed mostly of β‐D‐mannuronic acid (M)
and α‐L‐guluronic acid (G),38 and the combination and
arrangement of these molecules determine the properties of
the alginate—most importantly, how firm or how soft and
elastic a gel is formed.
Sodium alginate forms a fairly viscous solution in water,
typically 600 to 800 cps at 1 percent, and so it is sometimes
used as a thickening agent. Alginate does not require heat to
become viscous; it hydrates easily in a cold system and so it
can be used in products like instant beverage mixes.
The most commonly utilized attribute of alginate is its
ability to set instantaneously to a gel in the presence of cal-
cium, without applying heat. One of the most widespread
uses of sodium alginate is in formed foods, particularly onion
rings. As onions are processed into a paste or puree, sodium
alginate is added to it. It is then passed through an extruder
(like a doughnut extruder) that forms an onion cylinder,
which is sliced into rings. The rings drop into a bath contain-
ing a calcium solution, typically calcium lactate. The moment
the ring hits the calcium bath, it solidifies into a solid gel.
That gel is then battered and later fried.
This method of manufacture has many advantages. First,
there is no waste; when making traditional onion rings, the
ends are too small to use and are discarded. Second, each
onion ring is the same size as the next; this is especially
important in fast food restaurants, where portion control is
critical to cost savings. The onion paste can even be com-
bined with raw or frozen pieces of onion to give the onion
ring a more authentic feel. This treatment, with some varia-
tion in the machinery, is also used to make the pimientos
stuffed into green olives, the blueberries in muffin mixes, and
many other items. Surimi often uses sodium alginate to give
structural integrity to the formed fish items. Alginate gels are
not thermo‐reversible; they remain gelled even when heated.
This is advantageous when creating products that must
remain stable and hold together at elevated cooking temper-
atures (such as deep‐fried onion rings).
Another advantage to the calcium reaction is that the
time it takes to set the gel can be regulated through the addi-
tion of a sequestrant such as sodium tripolyphosphate, which
ties up the calcium for a while. When a dentist takes a dental
impression by placing a bite plate filled with gel in your
mouth, that gel might be a sodium alginate mixture. A
sequestrant is added so that, instead of gelling immediately
on contact with the calcium, the reaction is delayed long
c08.indd 157 1/14/2016 7:22:42 AM
158 • CULINOLOGY®
enough for the dentist to mix the powder with water, place it
in the bite plate, and get it into your mouth. Generally, it sets
to a solid gel within 2 minutes in your mouth.
This reaction is also used to great advantage in restau-
rants known for molecular gastronomy, where sodium algi-
nate is used to make such things as fruit caviars. The recipe
below uses sodium alginate in a similar fashion to create so‐
called “balls of energy” that can be floated on top of a drink.
Balls of Energy Cocktail
Ingredients
Alizé Gold Passion liqueur 50 mL
Charbay lemon vodka 50 mL
Non‐carbonated concentrated energy drink,
such as AMP
100 g
Sodium alginate 1 g
3 percent solution of calcium chloride 400 g
1 percent solution of carboxymethylcellulose
(CMC) in water
400 g
Sugar 5 g
Note: Not all energy drinks work here due to their ingredient
inclusions.
Procedure
1) Blend 1 g sodium alginate with 5 g sugar.
2) Mix the alginate/sugar blend into the energy drink with a
whisk. Let sit for 3 minutes.
3) Draw the alginate solution into an eyedropper or Pasteur
pipette. Squeeze drops into the calcium chloride solution.
Note: Hold the dropper above the solution, not immersed
in it.
4) When the alginate mixture hits the calcium chloride
solution, small balls of the energy drink immediately form.
Let them sit in the calcium chloride for about 10 minutes.
5) Use a fine sieve to remove the balls from the calcium
chloride solution. Rinse them off with fresh water.
6) Pour 50 mL Alizé into a martini glass. On top of that, pour
50 mL 1 percent CMC solution.
7) Place about 5 g energy balls on top of the CMC solution.
8) Pour 50 mL lemon‐flavored vodka on top of the CMC
solution. The balls float within the CMC layer and remain
there as the drink is consumed.
Propylene Glycol Alginate (PGA)
PGA is the result of reacting alginic acid with propylene
oxide. The single most distinctive attribute of PGA is that it is
a true emulsifier.
The most common use in the food industry is in salad
dressings and bottled marinades, where it usually paired with
xanthan gum. Xanthan gum provides body and texture and
acts as a pseudo‐emulsifier, but it is not a true emulsifier.
PGA provides true emulsification but is not as viscous as
xanthan and does not add as much body to a system.
Furthermore, PGA is far more expensive than xanthan, so
using it alone is not economical. Most salad dressings do well
with a 90:10 blend of xanthan and PGA. This combination,
used between 0.25 percent and 0.50 percent, contributes
body and mouthfeel and offers excellent emulsion stability.
PGA is always a good choice for an emulsion. However, it
cannot be declared natural, so its use in some products is
restricted.
The following recipe for taco meat uses PGA to help emul-
sify the water and fat from the meat. The konjac provides
body and helps hold in some of the water that is purged dur-
ing cooking. Prepare two samples, one with gums and a con-
trol without gums for comparison.
Taco/Burrito Filling
Ingredients
Ground beef chuck, 80
percent lean
500 g
Water 200 g
Chili powder 13 g
Dehydrated minced onion 10 g
Cumin, ground 4 g
Salt 3.5 g
Granulated garlic 2 g
Konjac gum 2.5 g
Propylene glycol alginate 1.5 g
Vegetable oil 15 g
Procedure
1) Put the beef in a pot. Add the water, chili powder, onion,
cumin, salt, and garlic to the beef.
2) Add the gums to the oil and stir to disperse.
3) Start heating. Whisk the gum/oil slurry into the beef.
4) Heat to 190°F (88°C) and hold for 15 minutes.
Because the sample using the gums is cohesive, it can be
accurately portioned out or flow evenly through machinery,
such as an extruder or piston filler. Going through an extruder,
the product with the gums is consistent in ingredient ratio
and weight; the control varies due to the water purge.
There is, of course, the added benefit of lower cost due to
the water being kept in the system and adding weight to the
serving portion.
Agar
Agar is the most ancient phycocolloid (a colloid extracted
from seaweed) still used as a gelling, thickening, and stabiliz-
ing food additive.39 It most likely started in Japan centuries
ago, and it is still used there as a primary food ingredient.
Agar comes from the class of seaweeds called Rhodophyceae,
as does carrageenan; however, the weeds used for agar are
primarily from the genera Gelidium and Gracilaria.
Agar was originally processed simply by boiling the sea-
weed. The result was an edible gel. An improved method of
preparation was, according to legend, discovered acciden-
tally when an innkeeper threw the leftovers of an agar jelly
outdoors, where it froze during the night and thawed the
c08.indd 158 1/14/2016 7:22:42 AM
CHAPTER 8 • Carbohydrate‐Based Foods • 159
next day. This resulted in a dry substance that, when recon-
stituted in water, resulted in a clearer jelly of better quality.40
Today dry agar strips are sold in stores.
Initially, agar should be heated to boiling (212°F [100°C])
for the greatest efficacy.
One of the major differences between agar and other gums
that form gels is that gelation, the point at which the liquid
becomes a solid gel, occurs at a temperature far below the gel
melting temperature. The gel does not set until it has cooled
down considerably, usually to about 86 to 95°F (30 to 35°C).
Once formed, the gel does not melt again until heated to 194
to 203°F (90 to 95°C). This makes agar ideal for one of its
widely used applications—cake and doughnut glazes.
Because the gel resets at a much lower temperature than
it melts, as long as the glaze temperature is kept fairly warm
(above 95°F [35°C]), it remains liquid. This allows glaze to be
used without fear of it setting too quickly, so doughnuts can
be dipped or cakes glazed under a cascade drip. With most
other gums, the gels reset at a much higher temperature
(closer to the melting point), so unless temperature is criti-
cally controlled, the gel may reset too soon. Furthermore,
because of its higher melting point, the glaze does not melt as
easily under high-heat conditions.
Other uses for agar include imitation fruit gels, water gel
desserts (it is not of animal origin and so can be used in
vegan, kosher, and halal applications), and the fining of wines
(the removal of suspended particles before bottling).
Agar from the Gelidium seaweed reacts synergistically
with locust bean gum to form a stronger and more elastic gel.
Gracilaria agar does not have this synergy; in fact, locust
bean gum may weaken the gel. On the other hand, gels made
with Gracilaria react better with sugars than those made
with Gelidium. The Gracilaria form is most often used for
bakery glazes and candy fillings.
In Asia, agar is used in much the same manner as gelatin
in the United States. Recently, a diet was introduced in Japan
that utilized the swelling properties of agar to create a feeling
of satiety. The diet was so successful that for some time after-
ward, agar was difficult to procure; Japan was buying up
almost all the product manufactured. Unlike pectin, agar
does not require sugar to create a gel, so sugarless jams and
jellies can be produced for dietary purposes.
Agar has also been used for years as the standard gelling
agent in solid media used to grow and identify bacteria,
yeasts, and molds, as well as a sterile propagation medium for
agriculturally important plants and plant experimentation.
Plant Gums of Terrestrial
Origin
Konjac
Konjac flour and konjac gum have been used as ingredients
in Asia for centuries; in Japan, they are often seen on a menu
as konnyaku. In Asia, noodles are produced from konjac
alone. Because they are made from a gum, konjac noodles are
much more elastic than traditional pasta.
The konjac plant is a tuber (like potatoes); however, it can
grow quite large, and it grows for at least three years before it
is harvested. The tuber is cut into slices and air‐dried to form
chips. It is then ground to a powder, and the starch and gum
are separated via either air classification or wet milling. In air
classification, the powder is blown on a fluid bed—a system
where air is blown under a screen so the powder is suspended
and the heavier particles (the gum) fall and the lighter parti-
cles (the starch) rise. In a wet system, the powder is made
into a slurry and passed through a centrifuge. The centrifuge,
like a clothes washer basket, has a liner in which the holes are
large enough to allow the smaller starch particles to pass
through but not the larger glucomannan (gum) particles.
After spinning, a cake of gum is left behind; this is dried and
sifted back into a powder.
Konjac is a glucomannan, similar to the galactomannans
(seed gums) but with a different monosaccharide (glucose
instead of galactose) on the side chains of the mannose
backbone.
Konjac has many characteristics, some unique, to
exploit. When dispersed into a solution with no heat, it
forms an extremely viscous solution (up to 36,000 cps at
1 percent—compare that to xanthan at 1500 cps at 1 per-
cent) with a short texture—that is, it pours much as a thick-
ened starch solution does. When poured, it does not have
the long, ropy tail often seen with other gums, and it exhib-
its a less gummy mouthfeel. However, unlike starches, which
it closely resembles in appearance, konjac does not mask
flavors. Also, because it is non‐ionic, it is relatively unaf-
fected by salts and is stable to a pH below 3.8, in some cases
as low as pH 2.8.41
When heated with xanthan or carrageenan, konjac forms
a gel that uniquely can be either thermo‐reversible or non‐
thermo‐reversible. After heating, if the pH is raised above
9.5 to 10.0, the gel is non‐thermo‐reversible. To create a
thermo‐reversible gel, simply do not raise the pH above 9.5.
Note that once the high pH is attained, the solution can be
brought back down to neutral or even acidic conditions. This
is important because so many packaged foods are acidified
or naturally acidic. Even milk is slightly acid, about pH 6.7.
Most fruits and vegetables are naturally acid—for example,
citrus fruits, tomatoes, and grapes. Some meats have a slightly
alkaline value, between pH 7.0 and 8.0, but again, nowhere
near the level needed to make a non‐thermo‐reversible kon-
jac gel. This is why we stress that the pH can be brought
down after it is raised. Most manufacturers add an alkali,
such as potassium hydroxide, to raise the pH of the konjac
solution. The pH is brought back down one of two ways. One
is through the addition of an acid, such as citric acid or phos-
phoric acid. More often, a small batch of konjac solution is
made, its pH raised, and it is added to a larger batch (a kettle
full of the remaining ingredients, for example) of low pH
product. Generally, the amount of konjac solution added to
the full kettle is so small that the pH of the total system is
relatively unchanged; thus, the konjac solution is now at the
same low pH as the entire system.
c08.indd 159 1/14/2016 7:22:42 AM
160 • CULINOLOGY®
Konjac absorbs a lot of water; it can pick up about 200
times its weight. (Xanthan and guar typically pick up about
40 times their weight.) This makes konjac a good choice
when moisture must be controlled.
Because it forms a non‐thermo‐reversible gel, konjac is an
excellent choice for products that must maintain their struc-
ture when heated, such as a vegetarian sausage and meat
analogs. Surimi utilizes konjac as a texturizing agent.
Like most thickeners that do not require heat, konjac is a
good choice for use in instant cold mix drinks. Because of its
short texture, which mimics starches, it can be used for
instant gravies and sauces as well.
When added to pasta dough (and treated to be non-
thermo-reversible), konjac helps maintain structure and
firmness when the pasta is placed on a steam table. It can
also be made into a reversible gel and used for frozen gravies
and items that require a slight gel for packing but must
reverse to a liquid when heated.
Konjac also has nutraceutical benefits, making it an
excellent choice for functional foods; it helps lower glyce-
mic index, cholesterol, and triglycerides. Konjac is just
becoming popular as an ingredient in the United States.
Some health food stores sell konjac in capsules; it is usually
labeled glucomannan. Asian supermarkets sell blocks of
gelled konjac for use in recipes. The Japanese version is cut
into strips and used as a noodle and topped with a sauce, or
made into cubes and used in a manner similar to tofu, in
salads or stir‐fried dishes.
The following recipe is a little tricky. The key is to use a flat
wooden spatula once the product starts to thicken and just
slowly push the paste as it boils. Do not mix fast, or the devel-
oping structure will break. Move it just enough to keep it from
burning.
Asian shops sell noodle presses into which a cube of fin-
ished gel is inserted and pressed to extrude noodles. If these
are unavailable, simply cut the finished gel into strips.
Calcium hydroxide is sold to the food industry as pickling
lime or hydrated lime.
Konjac Noodles
Ingredients Percent
Water 96.65
Konjac 2.35
Rice flour 0.85
Calcium hydroxide 0.15
Total 100.00
Procedure:
1) Add the calcium hydroxide to the water and mix until it is
in solution.
2) Blend the konjac and the rice flour together.
3) Whisk the konjac/rice flour blend into the water/lime
solution.
4) Heat to boiling while pushing the paste that forms so it
does not burn. Do not beat or whisk it; just keep it moving.
5) Once boiling, let the mixture boil for about 3 minutes
and then cover it and heat at low heat for another
5 minutes.
6) Place the resulting gel on a cutting board or cookie sheet
and refrigerate, preferably overnight.
7) Cut into ¼‐inch‐wide strips.
8) Steam the fresh noodles until hot and serve with an
Asian‐flavored sauce, such as a sesame peanut sauce,
on top.
Note that the noodles are non‐thermo‐reversible. That is
because using the calcium hydroxide brought the pH up to
about 9.5. Try the recipe without the calcium hydroxide and
see what happens when you steam the noodles.
Also note: The strong smell while preparing is typical. The
odor and taste disappear once the noodles are gelled and
steamed.
Cellulose Gums
Cellulose gums are chemically modified derivatives of cel-
lulose, the primary structural material in plant cell walls
and one of the most abundant compounds on earth. Most
cellulose derivatives start off as wood pulp or are derived
from cotton linters (the short fibers from the cotton boll).42
Depending on the chemicals used in processing, cellulose
can be changed into a wide variety of derivatives; in the food
industry, CMC is one of the most widely used of these
derivatives.
Carboxymethylcellulose (CMC)
One of the distinctive characteristics of CMC is its almost
crystal clear appearance when put into solution. It thickens
in cold water and does not require heat to be effective. CMC
is available in a very wide range of viscosities; from 200 cps at
1 percent up to 20,000 cps at 1 percent.
CMC is often used in frozen dairy‐based desserts because
it helps reduce ice crystal size and melting and dripping. CMC
is very widely used in frozen push pops and water ices for the
same reason. Without CMC, popsicles would be more like
solid ice cubes than softer pops that can be broken by biting.
Because of its clarity, CMC is often used to provide viscos-
ity in sugar‐free syrups. Without sugar, syrup has little or no
viscosity. CMC can replace the viscosity, and the resulting
solution is still clear.
CMC can also be used in baked goods to help stop spread.
Thickening pancake batter with CMC prevents it from
spreading on the griddle when poured. As improved water
binding is believed to inhibit fat absorption43, and CMC
is very good at binding water, it is often used for fried
doughnuts.
Try making the following ice pop recipe with and without
the CMC. Notice that after freezing, the control with no gum
c08.indd 160 1/14/2016 7:22:42 AM
CHAPTER 8 • Carbohydrate‐Based Foods • 161
is hard, like an ice cube, while the ice pop with the gum has
smaller crystals that can be bitten off.
Push‐up Frozen Ice Pop
Ingredient Percent
Sugar 12.00
CMC 0.10
Water 87.40
Citric acid 0.25
Flavor 0.25
Total 100.00
Procedure:
1) Blend the sugar and CMC.
2) Add the sugar mixture to the water; allow to mix and
hydrate for at least 5 minutes.
3) Add the remaining ingredients.
4) Place in small, freezable pouches and fold so the liquid
freezes into pops about 1 inch wide.
5) Freeze overnight.
Methylcellulose (MC) and
Hydroxypropylmethylcellulose (HPMC)
Two of the more unusual gums in this group are methylcel-
lulose (MC) and hydroxypropylmethylcellulose (HPMC),
which both form gels at high temperatures, whereas most
gums tend to become thinner at high temperatures. Some
grades gel as low as 120°F (50°C); some require greater than
195°F (909°C). Gelling at high temperature is called thermal-
gelation. These gums are useful as an additive to breading
and batters for products to be fried. When a batter contain-
ing them hits the hot oil, the gums immediately form a gel.
This barrier inhibits the oil from being absorbed into the bat-
ter or breading. The advantage is threefold: the batter is
crisper because it picks up less fat: it is less caloric, for the
same reason; and costs are lower because less oil is pulled out
of the fryer with the food.
Another use is in fruit fillings for pies; the thermalgelation
helps prevent boil‐out from the pie shell during baking;
boil‐out results in loss of product, a sloppy appearance, and
messy equipment.
In addition, CMC is a true emulsifier, with both hydro-
philic and hydrophobic receptors. This makes it useful for
sauces and salad dressings, much like PGA.
Neither MC nor HPMC can be used in products that claim
to be “all natural,” because they are derived through a chemi-
cal process.
Microcrystalline Cellulose (MCC)
Microcrystalline cellulose (MCC), or cellulose gel (also called
colloidal cellulose), is created by heating cellulose pulp in the
presence of a weak acid such as dilute hydrochloric acid. It is
then dried and ground. In this form it is called powdered
MCC. If it is blended with CMC prior to drying, it is called col-
loidal CMC.44
MCC is often used in low‐fat and fat‐free salad dressings.
After being subjected to high shear, the MCC particles form a
three‐dimensional network of crystals that gives foods a
short, gelled texture like that of fat to the tongue; it is func-
tionally similar to the structure of dispersed oil droplets in an
emulsion.45 If used at about 1.5 percent, the oil in a salad
dressing can be reduced by about half, and the result is simi-
lar in mouthfeel to a full‐fat type of dressing.
Pectin
Pectin is a familiar product to many chefs, bakers, and home
jelly‐makers. It is sold in supermarkets and used to make
homemade jams, jellies, and pie fillings. Most of the commer-
cial pectin used in the United States comes from citrus peels
or apple pomace (press‐cake remaining after juice is extracted
from the pulp), but pectin is found in most fruits and many
other plants. The two primary types of pectin, high methoxyl
(HM) and low methoxyl (LM), are sometimes referred to as
high ester and low ester.
High‐Methoxyl (HM) Pectin
High‐methoxyl (HM) pectin is the traditional, use‐at‐
home pectin. HM pectin requires high levels of sugar and
acid to form a gel. This is why it is used to create jellies
and jams, as well as fruit fillings for pies and other fruit
variegates.
HM pectins can form gels as wide‐ranging as a solid juice‐
based jelly to a soft marmalade. To set properly, pectins must
be used at the correct pH and with the correct level of sugar
solids. The three types of HM pectins are slow set (SS),
medium set (MS), and rapid set (RS), and the choice is based
on production needs and type of product. Because RS pectin
sets to a gel quickly, it might trap air bubbles created during
filling. To achieve a clear, bubble‐free product, use SS pectin
so the trapped air has time to escape before the gel sets. That
said, RS pectin is preferred in preserves, where fruit has a
tendency to float, to keep the fruit more evenly dispersed
throughout the jam matrix.
When using SS pectin, the pH must be between 3.4 and
3.8, depending on sugar content; in a rapid set it can go down
to pH 2.8. These variables make using pectin a bit tricky, and
in large production, very close attention must be paid to the
sugar solids and pH of incoming raw materials (such as fruits)
so adjustments in solids or acidity can be carefully calculated.
Pectin requires boiling in order to form a gel. In addition,
because HM pectin is not soluble above 20 percent solids but
requires 65 percent solids in order to gel, the process must be
designed so the pectin is introduced to the kettle when the
solids are low, but they must later be brought above 65 per-
cent. This is why the sugar is added after the pectin is already
dissolved and at full boil. Careful planning yields good
c08.indd 161 1/14/2016 7:22:43 AM
162 • CULINOLOGY®
results.46 The exact requirements for a particular product
should be discussed with the supplier.
Pectin has a protective effect on protein below its isoelec-
tric point (where it normally denatures, or starts curdling and
precipitating). This makes pectin useful in acidic milk‐based
products, such as pourable yogurt, and in soy‐based acidic
drinks, such as flavored soy milks, in that the addition of pec-
tin helps prevent the denaturation of the proteins. Acid dairy
and soy‐based products that go through high‐heat processing
such as HTST (high temperature, short time) thermal proc-
essing also benefit from the protection of HM pectin.
Low‐Methoxyl (LM) Pectin
Low‐methoxyl (LM) pectins do not require high sugar solids
content, nor do they require a low pH. To set to a gel they are
dependent on the amount of calcium available in the for-
mula; this can come from the food itself or be added as an
ingredient, such as calcium citrate. As a general rule, at least
60 mg calcium per gram of pectin is needed for the product to
set to a gel. However, this can vary, and amidated LM pectins
(those processed with ammonia) can set with lower levels of
calcium. Unlike HM pectins, LM‐amidated (LMA) pectins
create a thermo‐reversible gel.
In the case of LM pectins, calcium should not be present
until it’s time for the product to set, but it must be added at
some point prior to filling. The water used must be carefully
monitored for hardness (natural mineral content, including
calcium) to avoid unintended gelling.
That LM pectin does not require a high sugar content
makes it useful for low‐calorie applications. The gel is typi-
cally softer and more spreadable than that made with HM
pectin. The softer set of the LM pectin also makes it useful in
products such as fruit centers for confections and baked
goods. Like many other hydrocolloids, LM pectin also reduces
crystallization in frozen foods.
Inulin
Inulin is a type of long‐chain sugar molecule called a fructo‐
oligosaccharide, and it belongs to a category of fibers called
fructans. It is usually sourced from chicory or Jerusalem arti-
choke (which is a sunflower, not really an artichoke). Inulin
acts as a prebiotic—a substance that helps promote the
growth of useful bacteria (probiotics) in the gut. As it is not
digested in the mouth, stomach, or small intestine, by the
time inulin gets to the large intestine it is basically unchanged;
as such, it imparts only about 1.5 calories per gram.47 Because
it can be used at very high concentrations (up to 30 percent),
it is possible to use enough in a food to allow a nutritional
claim of active prebiotic. In fact, it is commonly added to
yogurts containing active cultures to add to the digestive
benefits of these products.
Inulin can be added as a cold solution; at about 7.5 per-
cent in water it remains a clear, low‐viscosity solution. At
about 15 percent, inulin begins to form a gel when heated and
cooled, and this increases in strength with the application of
shear, additional heat, and increased concentration. Another
method is known as seeding, in which a dilute inulin solution
is heated to a high temperature and, as it cools, more inulin is
added. This procedure, along with high shear, results in the
firmest type of inulin gel.48
The inulin gel is creamy and can mimic fat in low‐fat foods
such as dairy spreads and baked goods. It is also useful in
bakery fillings and fruit variegates when used at 5 to 6 per-
cent and mixed under high shear, which yields a smooth gel.
Less Used Gums
Many more gums are available for use in foods. Although
some have properties like the more familiar gums, they are
not widely used.
Furcelleran
Furcelleran is a marine gum from red seaweed of the
Rhodophycae family, like the carrageenans. It is sometimes
called Danish agar. As with many carrageenans, furcelleran
forms a gel when heated and cooled. It is most similar to
kappa carrageenans but often slightly darker in appearance.
At first the European Community (EC) regulators gave it its
own classification, E408, but later reclassified it as a carra-
geenan, E407, because it is so closely related. Note: “E Numbers”
are codes for substances that are approved for use as food
additives within the European Union and Switzerland. The
“E” stands for “Europe.” They are commonly found on food
labels throughout the European Union to help consumers
understand what ingredients are added to their foods. Having
a single unified list for food additives was first agreed upon in
1962 with food coloring. Any food item can be looked up by its
E Number. E100 to E199, for example, are colors, E200 to E299
are preservatives, and so on. Gums and emulsifiers fall into
the E400 to E499 range. This system saves label space because
the manufacturer can list numbers instead of ingredient
names.
Ghatti
Gum ghatti comes from trees grown in India and is similar to
exudates such as gum arabic and karaya, with a viscosity
range between those two. It is only about 90 percent soluble
and forms a dark solution. A true emulsifier, it is also con-
sidered a prebiotic because it arrives in the large intestine
largely unchanged and provides food for beneficial gut flora.
Gum ghatti is often used in syrups for buttering or adding
smoothness.
Cassia Tora
Cassia tora is an interesting weed, grown in India; a gum is
made from the endosperm of its seed. Like LBG and guar, this
gum is a galactomannan. The plant itself is used as a natural
pesticide on organic farms, and the seed can be roasted to
make a coffee substitute. Like many of the galactomannans,
cassia forms a gel when heated in the presence of xanthan
and certain carrageenans. Its widest use in the United States
is in pet food gels.
c08.indd 162 1/14/2016 7:22:43 AM
CHAPTER 8 • Carbohydrate‐Based Foods • 163
Stabilizer Blends
Gums can be very useful when combined with one another or
with other ingredients—such as calcium chloride, in the case
of sodium alginate. They exhibit a wide range of synergies
with other gums.
Vendors sell spice and seasoning blends, and flavor houses
sell compounded flavors. Similarly, suppliers make pre‐
blended stabilizing systems—although blends can certainly
be made in‐house. Keep in mind that more tools increase
your chances of success but also increase the amount of test-
ing that must be done. Most vendors carry several types of
guar, several of xanthan, dozens, if not more, of carrageenan,
and so on. These can be similar to one another or as far apart
as red and blue are on a color chart. Blending just the right
mixture yields the best texture at the best cost. Just as a flavor
chemist knows how to blend aroma chemicals and oils to
make up a particular flavor, a hydrocolloid specialist can cre-
ate a custom stabilizing blend for any application.
By law, each ingredient used in the stabilizer blend must be
listed separately on the label of the food product. This is
sometimes a consideration when a label is required to look as
clean as possible. Consumers have accepted gum ingredients,
for the most part, but too many can convey an over‐manufac-
tured impression. One other factor to consider when using
blends is the ratios are predetermined. Having a preset blend
may limit the number of food items that blend can be used in.
However, just as with unblended gums, blends can be used as
part of a larger stabilizing system and their use levels adjusted
accordingly.
Gelatin
Gelatin (spelled gelatine outside the United States) is not a
carbohydrate, it is a protein. Then why include it in the car-
bohydrate chapter? Although gelatin is a protein, it acts
much more like a carbohydrate hydrocolloid, and in food
products, it is almost always used for its hydrocolloid
functionality.
In fact, although gelatin is an animal‐derived protein, it is
not a complete protein as far as nutritive value is concerned.
While gelatin does contain 18 amino acids, a good percent-
age of the essential amino acids are missing or present in
inconsequential amounts. Gelatin is completely lacking in
one (tryptophan) and has almost none of another (methio-
nine). It is also low in isoleucine and threonine. This makes it
a poor choice to add protein to the diet.
Gelatin is made by breaking down collagen, a protein
found in the connective tissue of animals. It can be derived
from any animal, including fish, but most gelatin used in the
United States comes from either swine or cattle. It is not, as
commonly thought, commercially derived from hooves but
rather from hides and often bones as well.
Most gelatins used in the food industry are classified as
Type A or Type B. Type A is manufactured from swine and is
converted from collagen in a process involving acids. Type B
is manufactured from cattle using an alkaline conversion.
Typically, at equal concentrations Type A gelatin has a
stronger gel than Type B,49 but other textural issues, such as
elasticity, determine which type is used. The gel strength of
gelatin is measured as bloom strength. The bloom test
involves adding weight to a standard‐size plunger and meas-
uring the weight in grams required to make that plunger
depress a 6.66 percent gelatin gel to a depth of 4 mm at 50°F
(10°C). Typical bloom strengths are between 125 and 300,
with 300 bloom being relatively expensive due to its greater
gel strength.
One concern of many people when choosing gelatin as a
texturizing agent is that because it is derived from animals, it
cannot be considered for use in vegetarian or vegan foods. In
the same vein, as Type A is derived from swine and the source
of cattle for Type B cannot always be traced, the gelatin can-
not technically be considered kosher or halal. However, this
determination is usually left to the interpretation of the certi-
fying agency. Many vegetarian and some kosher and halal
supervisory organizations consider the process of heating
and alkalinization or acidification so severe that in the end
the gelatin has no relation physically to its source. For this
reason, some certifying agencies allow gelatin to be used in
kosher, halal, or vegetarian foods.
Although far less available and far more expensive, gelatin
from fish that are considered kosher is also available. It is
typically weaker than a Type A or Type B gel. Also available is
gelatin from kosher cattle herds that have been traced by a
supervising agency from birth through slaughter. Gelatin
made from these animals is approved kosher by all agencies.
Gelatin has been used for a very long time in the prepara-
tion of foods. Sometimes it appeared as the result of long
Tech Tips
Many, many variables are associated with the properties of
gums. To select the best gum sample for a specific applica-
tion, the gum supplier needs the following information:
1. What is the intended end use of the gum? That is, what is
the final product application?
2. What properties are expected of the gum? For example,
gelling, suspension, thickening?
3. What is the final food form? Is it frozen, retort, shelf‐stable,
refrigerated, or other?
4. Is it a high‐acid product (pH <4.5) or a low‐acid product
(pH >4.5)?
5. Will any ingredients or materials be available to help with
the dispersion of the gums? For example, oil, propylene
glycol, alcohol, salt, sugar, corn syrup?
6. Does the product need to be certified kosher, organic, all‐
natural, or any other profile?
7. Is the product heated, and, if so, to what temperature?
8. What machinery is available for dispersion and hydration?
For example, Scott Turbo® Mixer, Bredo Likwifier®,
Lightnin’® Blender, Eductor?
Having the answers to these questions enables the supplier
to home in on the best possible choices for the product.
c08.indd 163 1/14/2016 7:22:43 AM
164 • CULINOLOGY®
boiling of bones or skin or flesh with connective tissue. One
example is pot au feu, a beef stew where the meat contains
quite a bit of cartilage, such as oxtail. By cooking in water for
a long time, the collagen is converted to gelatin, which thick-
ens the stew. If left to cool, the stew forms a jelly (aspic, in
culinary terms). “Gefilte fish” is poached and then left to cool
in the broth in which it was poached. The broth, after chill-
ing, gels from the fish gelatin created by the poaching.
In the United States, gelatin is most frequently used in
powdered form, although it is available in sheets as well. It is
thermo‐reversible; it forms a gel if it is heated and left to cool,
but that gel breaks down at a lower temperature than most
other hydrocolloids. While this makes gelatin unsuitable for
items such as shelf-stable dessert gels (which must be made
with gums), it does have the beneficial characteristic of melt-
ing at below body temperature (less than 95°F [35°C]), caus-
ing the gel to melt in the mouth when eaten and thereby
providing a very cool and refreshing mouthfeel.
Gelatin is commonly used in yogurts for body and is the
main ingredient in many gummy bear–type candies. Of
course, when mixed with sugar, an acidulant, and flavor, it
becomes the water gel dessert commonly referred to by the
brand name Jell‐O®. Gelatin is also useful, when not made
into a gel, for maintaining foams in beverages. Marshmallows
rely on gelatin for just the right set and to hold and entrap the
air incorporated during their manufacture.
Gelatin is also commonly used for glazes and aspics, with
the gel strength determined by the amount of gelatin in solu-
tion (typically between 1 and 5 percent). Gelatin does break
down in the presence of certain proteases (protein enzymes),
so when using it in fruit‐based products, avoid fruits such as
pineapple, kiwi, and papaya.
Gelatin is also used as a protein encapsulant for an assort-
ment of functional ingredients to protect them from exposure
to harsh processing or storage environments or to provide
time‐release functionality. Protein encapsulation of oxygen‐
sensitive flavor oils prior to spray‐drying is one example.
Conclusion
The world of carbohydrates offers enormous possibilities
for the food developer. From moisture management to vis-
cosity enhancement; from freeze‐thaw stabilization to
emulsification, the list of functional capabilities is nearly infi-
nite. The keys to using carbohydrates successfully are to
clearly identify the desired attributes of the finished product
and to understand the properties of each carbohydrate ingre-
dient. As it is nearly impossible for any one individual to pos-
sess this encyclopedic knowledge, it is strongly recommended
that developers take advantage of their ingredient suppliers
to provide this critical information throughout the develop-
ment cycle, from project inception to product launch.
1USDA Dietary Guidelines for Americans 2005, retrieved from http://www
.health.gov/dietaryguidelines/dga2005/document/html/chapter2.
2C. L. Ogden, M. D. Carroll, M. A. McDowell, and K. M. Flegal, “Obesity Among
Adults in the United States: No Change Since 2003–2004,” NCHS Data Brief
No. 1 (Hyattsville, MD: National Center for Health Statistics, 2007). U.S.
Department of Health and Human Services, Healthy People 2010, 2nd ed.,
with Understanding and Improving Health and Objectives for Improving
Health, 2 vols. (Washington, DC: U.S. Government Printing Office,
November 2000).
321CFR101.9(c)(6)(ii).
4T. S. Temelkova‐Kurktschiev, et al., “Postchallenge Plasma Glucose and
Glycemic Spikes Are More Strongly Associated with Atherosclerosis Than
Fasting Glucose or HbA1c Level,” Diabetes Care 12 (December 23, 2000):
1830–1834.
5J. BeMiller and R. Whistler, Starch, 3rd ed., Chemistry and Technology. Food
Science and Technology, 2009.
6A.-C. Eliasson, Starch in Food: Structure, Function, and Applications (Cambridge,
England: Woodhead, 2004).
7R. Whistler, J. N. BeMiller, and E. F. Paschall, Starch: Chemistry and
Technology, 2nd ed. (Orlando, FL: Academic Press, 1984).
8BeMiller and Whistler, Starch.
9E. J. Pyler, Baking Science and Technology (Kansas City, MO: Sosland,
1988).
10Eliasson, Starch in Food.
11D. J. Thomas and W. A. Atwell, Starches (Eagan, 1999).
12G. Eggleston and G. Côté, “Oligosaccharides in Food and Agriculture,”
American Chemical Society, ACS Symposium Series, 2003.
13O. R. Fennema, S. Damodaran, and K. L. Parkin, Fennema’s Food Chemistry,
4th ed. (CRC Press, 2007).
14G. M. A. van Beynum and J. A. Roels, Starch Conversion Technology (Dekker,
Marcel, 1985).
15A. Srichamroen, C. J. Field, A. B. R. Thomson, and K. B. Tapan, “The
Modifying Effects of Galactomannan from Canadian‐Grown Fenugreek
(Trigonella foenum‐graecum L.) on the Glycemic and Lipidemic Status in
Rats.”
16Food and Agriculture Organization of the United Nations (FAO) Corporate
Document Repository, “Gums, Resins, and Latexes of Plant Origin: Gum
Arabic, Gum Talha, and Other Acacia Gums” (1995), retrieved from http://
www.fao.org/docrep/v9236e/v9236e05.htm.
17FAO, “Gums, Resins, and Latexes.”
18G. Phillips and P. Williams, Handbook of Hydrocolloids (Woodhead, 2000).
19S. Cui, Food Carbohydrates (CRC Taylor and Francis, 2005).
20A. Nussinovitch, Hydrocolloid Applications (Blackie Academic and
Professional, 1997).
21Y. Hui, Handbook of Food Science, Technology, and Engineering, vol. 3 (CRC
Press, 2005).
22G. Phillips and P. Williams, Handbook of Hydrocolloids (Woodhead,
2000).
23Nussinovitch, Hydrocolloid Applications.
24A. Imeson, Thickening and Gelling Agents for Food, 2nd ed. (Aspen
Publishers, 1999).
25I. Goldberg and R. Williams, Biotechnology and Food Ingredients (Springer‐
Verlag, 2007).
26United States International Trade Commission Rulings and Harmonized
Tariff Schedule, HQ 951034, July 6, 1992.
27Phillips and Williams, Handbook of Hydrocolloids.
28Cui, Food Carbohydrates.
29Ibid.
30Nussinovitch, Hydrocolloid Applications.
31Phillips and Williams, Handbook of Hydrocolloids.
32Cui, Food Carbohydrates.
33Nussinovitch, Hydrocolloid Applications.
c08.indd 164 1/14/2016 7:22:43 AM
CHAPTER 8 • Carbohydrate‐Based Foods • 165
34Ibid.
35A. Hoefler, Hydrocolloids (Eagan, 2004).
36Imeson, Thickening and Gelling Agents for Food.
37Ibid.
38Phillips and Williams, Handbook of Hydrocolloids.
39Imeson, Thickening and Gelling Agents for Food.
40Ibid.
41Ibid.
42Phillips and Williams, Handbook of Hydrocolloids.
43Ibid.
44Imeson, Thickening and Gelling Agents for Food.
45Ibid.
46Nussinovitch, Hydrocolloid Applications.
47Phillips and Williams, Handbook of Hydrocolloids.
48Ibid.
49A. Salvador and S. M. Fiszman, Journal of Dairy Science 81, no. 6, 1998.
c08.indd 165 1/14/2016 7:22:43 AM
9
166 • CULINOLOGY®
Lead Author: Marilynn Schnepf, Ph.D., R.D. , Professor, Nutrition and Health Sciences , University
of Nebraska
Lynne Morehart, Technical Service Manager , Cargill Oils and Shortenings
Brian K. Yager, Chef/Owner, Cuisine 256, LLC
Lipid‐Based Foods
Introduction
Lipid is the overall name for the class of nutrients known as
fats and oils . They are found in animal, marine, and plant
sources, where they provide insulation, energy, and nutrient
storage. In recent years, fats have received a lot of attention in
the press, much of it focused on limiting or eliminating their
consumption in general and certain fats in particular. Product
developers must understand the use of fats in products (and
diets) and how to select and utilize the proper fat for each
application. Not all fats are created equal.
Historical Perspective
Fats and oils are dense sources of calories in the human diet,
contributing 9 calories per gram, while proteins and carbohy-
drates provide only 4 calories per gram. The fats and oils in
earliest times came mainly from nuts and seeds, with occa-
sional animal sources. When finding and consuming enough
calories was a challenge, the energy density of fats and oils
was a positive characteristic.
Eventually, through trial and error, early people discovered
how to extract fats and oils from their sources to use in cook-
ing and food processing. However, as enhanced shelf life and
organoleptic properties were desired, lipids went through
new processing techniques to provide greater resistance to
rancidity and improved texture and functionality.
During the last century, research has shown how the
sources, processing, and quantities of fats and oils in the diet
affect health and product development. In many countries
where an abundance of calories is available for consumption,
the high number of calories in fat is no longer an advantage
and now is often seen as a liability. As a result, significant
changes have been made to fats and oils to provide the con-
sumer with better‐tasting and more healthful products.
Fat Sources
Lipids come from animal, marine, and plant sources. The
most notable animal fats are lard (from pigs), tallow (from
beef cattle), and butter; fish provide marine oils, and plants
provide corn, soy, olive, peanut, sunflower, cotton, and
numerous specialty oils such as sesame, nut, and other seed
oils. Most of the plant sources are classified as polyunsatu-
rated, while the land animal sources are more saturated. The
exceptions are the tropical oils—coconut, palm, and palm
kernel—which are from plant sources but contain saturated
fatty acids. Although fish oils are from animals, they tend to
be more unsaturated. All animal fats, including marine
sources, contain cholesterol; plant‐derived fats do not. A vast
array of choices are available to product developers, who
must choose among them carefully.
Vegetable Oils
Vegetable oils are the lipid materials derived from plants,
primarily their seeds. Most are composed primarily of unsat-
urated fatty acids, either mono‐ or polyunsaturated, and as
such are liquid at room temperature and tend to have higher
smoke points than animal fats. Examples include soy, corn,
canola (rapeseed), peanut, cottonseed, avocado, safflower,
sunflower, almond, olive, and sesame seed oils. All of these
oils have smoke points at or above 400°F (204°C). The animal
fat with the highest smoke point is lard, at 375°F (191°C).
This makes vegetable oils the preferred medium for deep
frying.
Tropical oils (those extracted from coconut or oil palms)
and cocoa butter are composed primarily of saturated fatty
acids, and as such are solid at room temperature. They are
excellent in baking applications as a replacement for animal
fats and hydrogenated vegetable shortenings, and although
c09.indd 166 1/22/2016 1:09:13 PM
CHAPTER 9 • Lipid-Based Foods • 167
they are a source of saturated fat in the diet, they contain
neither cholesterol nor trans fats.
As previously noted, nutritionists agree that a healthy diet
should contain a ratio of omega‐6 to omega‐3 fats of no more
than 4:1. The average modern American diet has been esti-
mated to have a ratio of between 10 and 20:1. Recent findings
indicate that a diet overly high in omega‐6 fatty acids negates
the positive effects of omega‐3 fatty acids, and in fact may
increase the incidence of a number of diseases.
Unfortunately, nearly all vegetable oils contain an over-
abundance of omega‐6 fatty acids, and a shortage of omega‐3
fatty acids. For example, cottonseed oil, a common ingredient
in cooking oils, has a ratio of 234:1 omega‐6:omega‐3; corn oil
has a ratio of 46:1 omega‐6:omega‐3; even soybean oil has an
8:1 ratio. This is of concern because there are so few good
sources of omega‐3 fatty acids in the diet (primarily seafood,
and to a much lesser extent, walnuts and flax seeds) to help
balance the ratio between the two, and vegetable oils (and
foods prepared from them) have become such a large part of
the diet in developed countries.
On the healthier side, canola oil has a ratio of 2:1 omega‐
6:omega‐3. Clearly, new product developers for companies
interested in healthier foods should opt to use canola oil
whenever a vegetable oil is called for.
Animal Fats
Fats associated with beef, pork, lamb, turkey, and chicken are
more saturated and are solid at room temperature. These fats
are made up of many fatty acids, as are all fats and oils, but
palmitic acid (C‐16) and stearic acid (C‐18) tend to be present
in high amounts. Lamb fat is the most saturated, followed by
beef, pork, and chicken. Nutrition studies show that not all
saturated fatty acids have negative health consequences. For
instance, stearic acid tends to be neutral; it does not raise
LDL cholesterol or lower HDL cholesterol levels like other
saturated fatty acids, such as palmitic acid.1
Animal fats do impart a flavor (often mild) to the foods
they are added to and when they are used for frying. Pie crust
made with lard is described as having a nutty (not porky) fla-
vor. (Lard contains the optimum ratio of saturated and
unsaturated fatty acids to create a pie crust that is both flaky
and tender.) Lamb fat and turkey fat, in particular, are very
strong and characteristic of the source animal. Animal fats
are often added to formulated meat products like meatballs,
hot dogs, chicken nuggets, sausages, and deli meats to
enhance flavor, moisture, and texture, and to carry other fat‐
soluble flavor compounds.
Fish oils contain highly unsaturated fatty acids, often with
four or five double bonds. Because many fish live in cold
water, their fat must remain fluid at cold temperatures.
Highly unsaturated fats do not solidify at cold temperatures.
Unfortunately, the numerous double bonds in fish oils allow
rapid oxidative rancidity once extracted, which means a very
short shelf life. The omega‐3 fatty acids in fish oil are valued
for their important nutritional properties, but it is difficult to
incorporate them into food products because of their limited
shelf life and fishy flavor. However, suppliers of fish oils are
working on deodorizing the oils and encapsulating them to
prevent oxidation while maintaining their beneficial nutri-
tional aspects.
Dairy Fat (Butter)
The natural fat content of milk varies from 3.5 to 5.2 percent
according to the breed of cow. Milkfat is obtained by taking
advantage of the difference in density between fat and water.
First the cream is separated from whole milk with a centrifugal
cream separator. The result is added back to skim milk at vari-
ous levels to make milk products with different fat contents.
The cream, which is an oil‐in‐water emulsion, is then
churned to make butter (a water‐in‐oil emulsion). This process
uses mechanical energy to rupture the milk membranes
surrounding the fat droplets in the emulsion, allowing them
to coalesce into ever‐larger clumps of fat (butter grains). To
maximize process efficiency, the cream is chilled first, as the
fat droplets coagulate more easily when they are cold (think
about refrigerated butter). Once churning is complete, the
solid butterfat is separated from the fat‐free ‘buttermilk’ and
is kneaded to remove as much of the remaining liquid as pos-
sible; kneading also aligns its crystalline structure for a
smooth texture. The buttermilk that results bears no resem-
blance to commercial buttermilk, which is normally cultured
(like yogurt) prior to bottling for sale.
Salt is commonly added for flavor and to help preserve the
butter by preventing the growth of microorganisms. Sweet or
unsalted butter is also widely available but must be kept refriger-
ated for maximum shelf life. It is also interesting to note that
sweet butter normally has a natural flavor added to it (starter
distillate) to enhance its flavor; salted butter does not. European
“cultured butter” is made with cream that is fermented first to
give it a slightly tangy taste. European‐style butter is 82 percent
fat—slightly higher than standard American butter. (The U.S.
FIGURE 9.1 Fat percentage by weight of milk
and cream.
Milk or Cream Product Fat Percentage by Weight
Whole milk 3.25 minimum
Low-fat milk 1
Reduced-fat milk 2
Sweetened condensed milk 8 to 10
Half-and-half 12
Light cream 18 to 30, usually 20
Light whipping cream 30 to 36
Heavy cream 36 to 40
Double cream 42 to 48
Clotted cream 55 to 60
Source: Brian Yager.
c09.indd 167 1/14/2016 7:26:50 AM
168 • CULINOLOGY®
Standard for butter calls for a minimum of 80 percent milkfat,
with the remainder made up of approximately 15 percent water
and 5 percent milk solids—proteins and lactose.)
The fatty acids in butter are a mixture of short‐ , medium‐ ,
and long‐chain fatty acids, mostly saturated, except for a
fairly large slug (about 30 percent) of oleic (monounsatu-
rated). This unique combination of mostly saturated fatty
acids makes butter a solid (yet soft) fat at room temperature
and gives it a melting point close to body temperature; it
melts in the mouth, releasing its flavor compounds instanta-
neously. The narrow melting range for butter makes it diffi-
cult to incorporate into baked products: straight out of the
refrigerator it is too hard, and at room temperature it can eas-
ily become too soft. It does not have the broad range of plas-
ticity of synthetically made margarines and shortenings. On
the other hand, butter gives products a readily identifiable
mouthfeel and flavor that is difficult to replicate, a difficult
challenge facing margarine manufacturers.
Margarines were first developed as a more stable and less
expensive substitute for butter by a French chemist in 1869
and called oleomargarine after the Latin word for oil, oleo, and
its major fatty acid constituent, margaric acid (which today we
know is actually a combination of stearic and palmitic acids).
Margarines were typically made by blending hydrogenated
oils with liquid oil and adding milk solids and water to get the
desired texture, flavor, and functional properties. Unfortun-
ately, as we now know, the process of partial hydrogenation
causes trans fats to form, so many margarines today are for-
mulated without partially hydrogenated fats. Most margarine
is made from soybean oil, but some are made with corn oil or
blends of other plant oils. Plant oils contain long‐chain unsat-
urated fatty acids, unlike butter; thus, the texture and mouth-
feel of margarines are not the same as butter. However,
because they are more plastic, with a wider melting range, it is
DIETARY FAT Comparison of Dietary Fats
1
16
71
57
9
54
54
9 1
10
3 1
33
Flaxseed oil
Sunflower oil
Corn oil
Olive oil
Soybean oil
Peanut oil
Cottonseed oil
Lard
Palm oil
Butter
Coconut oil
*Trace Fatty acid content normalized to 100%
SATURATED FAT POLYUNSATURATED FAT
linoleic acid
(an omega-6 fatty acid)
alpha-linolenic acid
(an omega-3 fatty acid)
oleic acid
(an omega-9 fatty acid)
MONOUNSATURATED FAT
9
12
13
15
15
19
27
43
51
68
91
Canola oil 21 117 61
114Safflower oil 8 77
18
16
29
75
238
1
1
57
48
19
47
39
S
O
U
R
C
E
:
P
O
S
P
IL
O
T
P
L
A
N
T
C
O
R
P
O
R
A
T
IO
N
28
72
FIGURE 9.2 Comparison of dietary fats.
Source: Printed with permission from the Canola Council of Canada.
FIGURE 9.3 Lipid comparison.
Lipid Source
%
Saturated
% Mono‐
unsaturated
% Poly‐
unsaturated
Fats
Lard Animal 40.8 43.8 9.6
Butter Animal 54.0 19.8 2.6
Tallow Animal 50.0 43.0 4.0
Oils
Soy Plant 14.5 23.2 56.5
Coconut Plant 85.2 6.6 1.7
Corn Plant 12.7 24.7 57.8
Sunflower Plant 11.9 20.2 63.0
Safflower Plant 10.2 12.6 72.1
Olive Plant 14.0 68.7 11.2
Palm Plant 45.3 41.6 8.3
Wheat
germ
Plant 18.8 15.9 60.7
Source: Brian Yager.
c09.indd 168 1/14/2016 7:26:51 AM
CHAPTER 9 • Lipid-Based Foods • 169
easier to incorporate them into baked products. Modern tech-
nology allows lipid chemists to develop a range of specialty
margarines custom designed for virtually every commercial
application that would have previously used butter, without
butter’s “shortcomings.”
Criteria for selecting the best fat for any new product include
cost, animal or vegetable source (which also affects price), fla-
vor requirements (or lack of flavor), functional requirements,
labeling requirements, and nutritional considerations. The
decision process can be a daunting one that must be based on
experience and knowledge. Suppliers of fats and oils are profi-
cient at providing numerous custom‐designed products that
meet the needs of developers and can be enormously helpful in
making optimal fat choices.
Lipid Structure
The primary difference between fats and oils is whether they
are liquid or solid at room temperature. Oils are liquid at
room temperature, whereas fats are solid. One determinant
depends on the degree of saturation around the carbon
bonds in the fatty acids. Oils are liquid at room temperature
because the fatty acids have more unsaturated bonds; the
reverse is true of fats. Shortening is oil that has been partially
hydrogenated (had hydrogen added to it) to make it solid and
more stable. In this process, double bonds are lost and
the degree of saturation is increased. In general, the longer
the fatty acid chain, the more solid the fat and the higher the
melting point. Fatty acids with very short chains are more
volatile (vaporize at relatively low temperatures and are
highly aromatic).
Of all the lipids in nature, the most important in the devel-
oper’s daily work are the triacylglycerides, also known as
triglycerides. Most fats in food are in the form of triglycerides.
FIGURE 9.4 Fatty acids in various food products.
Name
# of
Carbons
# of Double
Bonds Source
Melting
Point (°F)
Butyric 4 0 Butter 18
Palmitic 16 0 Palm and
coconut
145
Stearic 18 0 Red meat 157
Oleic 18 1 Plant 55
Linoleic 18 2 Plant 23
Linolenic 18 3 Plant 12
Source: Brian Yager.
FIGURE 9.5 Fatty acid comparison by source of lipid.
% of Fatty Acid Found in Different Fat Types
Mono‐
unsaturated
Polyunsaturated
Fat Type
Smoke
Point
(°F)
Saturated
Linoleic Linolenic
Eicosa-
pentaenoic
(EPA)
Docosa-
hexaenoic
(DHA)Butyric Lauric Myristic Palmitic Stearic Oleic
C4:0 C12:0 C14:0 C16:0 C18:0 C18:1 C18:2 C18:3 C20:5 C22:6
Butter oil 350 3.6 2.9 10.8 26.9 12.1 28.5 3.2 0.4
Canola 400 4.1 1.8 60.9 21.0 8.8
Cocoa butter 26.3 33.8 34.3 3.1
Coconut oil 350 47.1 18.5 9.1 2.8 6.8 1.9
Corn oil 450 10.9 2.0 25.4 59.6 1.2
Fish oil 235 9.3 17.1 2.8 11.4 1.5 1.6 15.5 9.1
Lard (pork fat) 370 1.5 26.0 13.5 43.9 9.5
Palm oil 420 1.0 44.4 4.1 39.3 10.0 0.4
Peanut oil 440 11.1 2.4 46.7 32.0
Safflower oil 450 6.8 2.3 12.0 77.7 0.4
Soybean oil 450 10.6 4.0 23.3 53.7 7.6
Sunflower oil 440 7.0 4.5 18.7 67.5 0.8
Tallow (beef fat) 420 3.2 24.3 18.6 42.6 2.6
Source: Brian Yager.
c09.indd 169 1/14/2016 7:26:51 AM
170 • CULINOLOGY®
Their basic structure consists of a glycerol backbone with
three fatty acids connected (“esterified”) to the glycerol back-
bone by ester linkages. The specific fatty acids arranged on the
glycerol backbone, their chain length, their degree of satura-
tion, and the presence or absence of cis or trans hydrogens
give each triglyceride its physical, chemical, and functional
characteristics. Generally, the triglyceride is laid out like a
three‐pronged fork. The fatty acid chains (the three tines on
the fork) are attached to a glycerol backbone. Each chain can
be the same fatty acid, or they each can be different.
The backbone of fatty acids found in foods is made up of 4
to 18 carbons (some found in fish are 20 to 24 carbons long).
Fatty acid chains usually contain even numbers of carbons.
For stability, each carbon atom prefers to have four other
molecules attached to it. With four molecules attached, the
carbon atom is at its most stable and less likely to react with
other molecules. In the case of saturated fatty acids, each
interior carbon in the chain is attached to two other carbon
atoms and two hydrogen atoms. If the carbon atom is located
at one end of the fatty acid chain, it is attached to one other
carbon and three hydrogen atoms; if it is located on the oppo-
site end of the chain, it is attached to one other carbon and a
carboxyl (COOH) group.
When the carbon chain that makes up a fatty acid has all
of its binding sites filled, it is termed saturated. However,
when some of the hydrogen is removed, double bonds form
between adjacent carbon atoms and the fat is termed
unsaturated. These unsaturated fatty acids can be further
classified as monounsaturated (chains containing only one
unsaturated double bond) or polyunsaturated (chains con-
taining two or more unsaturated double bonds).
The remaining two hydrogen atoms associated with the
double‐bonded carbons are located either on the same side
of the chain (cis fatty acid) or opposite from each other (trans
fatty acid). Although natural sources of trans fats do exist,
most dietary trans fats are a result of processing—either
through hydrogenation (the chemical process of adding
hydrogen atoms to unsaturated double bonds in order to
saturate them) or as a result of the deodorization process.
Structure‐wise, trans fatty acids are straight and can pack
together tightly (allowing them to behave more like saturated
fats), whereas cis fatty acids contain kinks that prevent them
from packing tightly together.
As a result, trans fats are solid at room temperature. This is
one reason vegetable shortenings are solid at room tempera-
ture. Recent scientific evidence associates the consumption of
trans fats with an increased risk of developing heart disease
(among other findings). Therefore, since 2006, the government
has mandated that the amount of trans fat in a product be
labeled on the nutrition facts panel on all packaged foods.
Many companies have worked diligently to remove trans fats
from their products now that they are widely recognized as
harmful to health (and harmful to sales!). The U.S. Food &
Drug Administration is considering banning trans fatty acids
completely.
All lipids are made up of a variety of fatty acids, each hav-
ing different degrees of saturation and different chain lengths
and therefore different properties, including melting point.
The ratio of saturated to unsaturated fatty acids in any lipid
determines its melting point. A lipid with more unsaturated
fatty acids tends to be more liquid at room temperature; one
with more saturated fatty acids tends to be more solid.
The Solid Fat Index (SFI) and Solid Fat Content (SFC) of
any given lipid provide the percentage of fat in that lipid that
is in crystalline (solid) form compared with total fat (the
remainder being in liquid form) at a series of standardized
temperatures. The resulting data gives the product developer
Saturated
CH3-CH2-CH2-CH2-CH2-CH2-CH2-CH2-CH2-CH2-CH2-CH2-CH2-CH2-CH2-CH2-CH2-COOH
Stearic Acid (18:0)
Monounsaturated
Oleic Acid (18:1)
Polyunsaturated
Linoleic Acid (18:2)
CH3-CH2-CH2-CH2-CH2-CH2-CH2-CH2-CH CH-CH2-CH2-CH2-CH2-CH2-CH2-CH2-COOH––
CH3-CH2-CH2-CH2-CH2-CH2-CH2-CH2-CH CH-CH2CH CH-CH2-CH2-CH2-CH2COOH
–– ––
FIGURE 9.6 Saturates versus unsaturates.
Source: Brian Yager.
cis
trans
C
H
H
C
H
H
C
H
C
H
H
C
H
C
H
H
C
H
H
C
H
H
C
H
H
C
H
H
C
H
H
C
H
H
C
H
H
C
H
H
C
H
C
H
FIGURE 9.7 Cis versus trans bonds.
Source: Brian Yager.
c09.indd 170 1/14/2016 7:26:51 AM
CHAPTER 9 • Lipid-Based Foods • 171
a “snapshot” of the lipid’s physical and chemical make‐up and
behavior, allowing for better decision making during the
ingredient selection process when new lipid‐containing
products are being developed. The primary difference
between the two measurements is the methodology and
equipment used to determine the data, which is statistically
the same via either method.
Figure 9.8 shows the properties of the various lipids: satu-
ration, chain length, and orientation of double bonds. The oil
processor can predict the physical and chemical behavior of a
fat based on the types and ratios of the fatty acids present in
its composition.
Processing of Fats
and Oils
The initial processing of fats and oils involves their extraction
from the natural food source. Lipids from animal sources are
melted by heating with steam and then separated by centrif-
ugation. This process, called rendering, is used to extract lard
and tallow. Milkfat (butter) is separated from cream by
churning, which divides the skim milk (buttermilk) from the
fat by mechanical action and turns an oil‐in‐water emulsion
(cream) into a water‐in‐oil emulsion (butter).
The lipid component from plant sources is extracted by
pressing or solvent extraction. Pressing is a mechanical pro-
cedure that involves crushing the oil source (typically seeds)
and separating the fats by direct compression, then filtering
to remove impurities. Depending on the oil, heat may be
applied to inactivate enzymes such as lipases or lipoxygen-
ases that may promote lipid rancidity. This method is not as
efficient as solvent extraction; consequently, pressing is more
expensive. However, pressing is often preferred by people
concerned about organic solvents and the potential for resid-
ual chemicals to be left in solvent‐extracted oils. Terms found
on labels of pressed oils may include “expeller pressed ” and
“cold pressed.” Many oils sold in health food stores are
extracted using this method.
Solvent extraction involves the use of an organic solvent,
such as hexane, to dissolve the lipid. The solvent is then
separated from the lipid by distillation (the solvent has a
lower boiling point than the oil). At this point the oil is termed
crude oil. The crude oil contains mainly triglycerides but also
phospholipids, free fatty acids, and pigments, which are
impurities that can decrease the shelf life. The removal of
those impurities is termed refining.
The goal of refining is to remove compounds that may
cause undesirable odors, colors, or flavors and may shorten
shelf life. The first step is often degumming, which is neces-
sary for oils high in phospholipids (such as soybean, corn,
and sunflower oils). In the degumming process, 3 to 5 percent
water is mixed with the oil, the water‐soluble heads of the
phospholipids bind with the water, and the hydrated phos-
pholipids are separated from the oil by centrifugation.
Phospholipids are similar to triglycerides except one fatty
acid side chain is replaced by a phosphate‐containing hydro-
philic molecule. This makes it an “amphiphilic compound,”
i.e., a true emulsifier with both a hydrophilic head and a lipo-
philic tail. Besides lecithin’s ability as an emulsifier, having
both water‐loving and fat‐loving parts also makes it the ideal
molecule to form the membranes (lipid bilayers) that
surround every living plant and animal cell. Phospholipids
make up 75 percent of every cell membrane’s composition.
Neutralization removes free fatty acids from the crude oil.
Caustic soda (a strong alkali solution) is mixed with the
heated fat and allowed to stand to separate out the aqueous
phase, which traps the free fatty acids. This aqueous phase is
called foots or soapstock and in fact is used for making soaps.
Soapstock is removed from the triglycerides by adding water
and subjecting the mixture to centrifugation.
The oil is then bleached by passing it through diatoma-
ceous earth—clays or activated charcoal—that absorbs the
colored compounds. These include carotenoids (yellow to
red) and chlorophylls (green). By manipulating the bleaching
step, the processor can determine how much pigment to leave
in the oil. Corn oil traditionally has a deeper yellow color than
soybean oil. Olive oil may have more green pigments.
Small odor‐causing compounds (usually ketones, alde-
hydes, or free fatty acids) are removed by a process called
deodorization. In this step, steam heat in a vacuum chamber
strips away these low‐molecular‐weight compounds. The
FIGURE 9.8 Solid fat content comparisons.
Temperature Butter Cocoa Butter Coconut Oil Lard Palm Oil Palm Kernel Oil Tallow
SFC 10°C (50°F) 52 84 48 53 72 66
SFC 21.1°C (70°F) 22 70 34 27 24 39 43
SFC 26.7°C (80°F) 10 0 17 13 10 31
SFC 33.3°C (92°F) 4 NA 5 6 NA 19
SFC 40°C (104°F) NA NA NA 2 NA NA 9
Melting point 100°F 88–92°F 80°F 100°F 103°F 85°F 115°F
Lard and tallow SFCs will vary based on diets of animals.
Source: Brian Yager.
c09.indd 171 1/14/2016 7:26:51 AM
172 • CULINOLOGY®
resulting refined oil contains mainly triglycerides plus resid-
ual pigments and tocopherol (vitamin E). The tocopherols
that survive the refining process help preserve the oil and
delay the onset of rancidity. The oil should have almost no
odor, as triglycerides are large compounds and not volatile,
therefore not aromatic.
Refined oil can be further processed to create tailor‐made
fats and oils with specific functional properties. Solid fats are
often preferred for optimum texture and shelf life in many
baked products; traditionally, bakers used lard. However, the
negative health effects of consuming saturated animal fats
containing cholesterol created a demand for a healthier,
plant‐based substitute that retained solid fat functionality.
Hydrogenation is a method for achieving both objectives. It is
a common processing technique used to turn oils (mono‐ or
polyunsaturated) into solid fats by adding hydrogen atoms
wherever there are unsaturated carbon atoms. This reduces
the number of unsaturated fatty acids prone to lipid oxida-
tion and rancidity, thereby increasing the shelf life. A nickel
catalyst is usually used to facilitate the reaction.
Partial hydrogenation of oils yields a softer, smoother fat
(shortening) of the right texture and flexibility required in a
baking fat. During this process, some of the double bonds are
not removed but merely changed from the cis form to the
trans form. Partially hydrogenated fats are the number‐one
source of trans fats in the diet—and, as we now know, con-
sumption of trans fats should be reduced. Complete hydro-
genation, whereby all of the carbon‐carbon double bonds are
eliminated and replaced with hydrogen atoms, yields a fully
saturated fat. This type of complete hydrogenation yields a
hard fat that can be combined with oil to yield a more pliable
solid fat that is trans‐fat free.
Another processing technique is winterization. The oil is
refrigerated to between 32 and 35.6°F (0 and 2°C), causing the
more saturated long‐chain fatty acids (LCFA) present to crys-
tallize or precipitate out of the oil. When the LCFAs are
removed, the winterized oil remains clear at refrigerator tem-
peratures. This process is important for oils used in refriger-
ated products, such as salad oils and mayonnaise. If a
non‐ winterized oil is used in these products, the texture may
change when the product is refrigerated. In vinaigrettes, for
example, the appearance may become cloudy and mouthfeel
may become gritty. The term winterization comes from the
practice of letting cottonseed oil stored in barrels remain out-
doors during the winter in the South. In the cold temperatures,
the liquid fraction separated from the solid fraction due to dif-
ferent unsaturated and saturated fatty acids in the cottonseed
oil. The solid fraction was more saturated and could be used in
baked products where a more solid fat was needed, and the
liquid fraction was used in any application calling for liquid oil.
Fractionation, a process much like winterization, also cre-
ates different types of oil by separating fractions with differ-
ent melting points. It separates solid and liquid phases by
subjecting the oil progressively to different controlled tem-
peratures and removing each of the phases by vacuum or
pressure filtration. For example, milkfat (butter) is hard and
difficult to work with at refrigerator temperatures. The fat
can be separated into fractions based on the melting points
of the various fatty acids normally present in it, and the frac-
tions can then be recombined to create a butter that is
spreadable at refrigerator temperatures.
The location and types of the three fatty acids on the glyc-
erol backbone determines how the triglycerides interact,
resulting in crystal formation. The number and size of crys-
tals formed determine how smooth and creamy a fat is. Many
types of fat crystals can co‐exist in solid fats because
of the many fatty acid chain lengths and their positions on
the glycerol backbone. This is known as polymorphism.
However, three main forms are alpha (a), beta‐prime (bʹ), and
beta (b). The melting point of fats rises as the crystal sizes
increase from alpha to beta‐prime and eventually to beta.
Alpha crystals are extremely fine but not very stable. Beta
crystals are large and stable but give the fat a coarse, grainy
texture. Beta‐prime crystals are intermediate in size and rea-
sonably stable. Beta‐prime crystals are best for food prepara-
tions because they yield fine‐textured baked goods and
smooth‐surfaced hydrogenated vegetable shortenings.
To change the natural crystal formation of solid fats, fatty
acids can be moved around on the glycerol backbone. This
interesterification process is done by using catalysts and
lipases (enzymes that target fats) to remove the fatty acids
from the glycerol backbone and move them to another posi-
tion on the glycerol molecule. Unprocessed lard is a good
example of a fat with primarily beta crystals, which give it a
waxy, lumpy, grainy texture. Lard is often interesterified to
change the natural beta crystals into beta‐prime crystals.
Once it is interesterified, lard is whiter, creamier, smoother,
and more uniform in texture. Functionally, beta‐prime crys-
tals help with the incorporation of an abundant quantity of
small air bubbles in batters for good volume, texture, and ten-
derness of baked goods.
Plasticizing refers to softening a hard fat, resulting in a
change in its consistency due to a change in crystal structure.
When a solid fat is melted, the rate of cooling and the amount
of stirring affect how the fatty acid chains can interact to
form crystals. This process is used to achieve the proper
texture of tub margarines. Again, the desirable crystals are
beta‐prime, which are achieved by proper tempering and
temperature control.
A defect known as bloom appears as a whitish film on the
surface of chocolate when the crystal structure changes from
small, fine alpha crystals to large, coarse beta crystals. The
tempering process used in chocolate‐making delays this
change. One form of tempering is done by melting the choco-
late (which must contain cocoa butter) to 110°F (43°C) and
then mixing in a portion of unmelted chocolate pieces.
Cutting the chocolate into small pieces allows it to melt
evenly to ensure proper beta‐prime crystal formation.
Trans Fat and Hydrogenation
Dietary trans fats have been linked to increased risk of cardio-
vascular disease, hypertension, diabetes, and possibly neuro-
degenerative disorders. Because of the overwhelming scientific
c09.indd 172 1/14/2016 7:26:51 AM
CHAPTER 9 • Lipid-Based Foods • 173
evidence linking trans fats to cardiovascular diseases,
beginning in January 2006, the FDA required all food labels to
disclose the amount of trans fat per serving in the nutrition
facts panel. In the United States, if the amount is less than 0.5 g
per serving it still must appear on the nutrition facts panel, but
it can be reported as 0. Some municipalities and states ban the
use of trans fats in restaurants, and more states are considering
similar bans. Trans fats are an unfortunate consequence of the
hydrogenation process.
For example, the linolenic fatty acid (ALA) (an omega‐3
essential fatty acid containing three double bonds) content of
soybean oil must be reduced to increase the oil’s stability if it
is to be used as a frying oil. This is because ALA is a polyun-
saturated fatty acid and very susceptible to oxidative rancid-
ity. When this happens, the soybean oil initially develops
green‐beany or grassy flavors, and in later stages strong fishy
or painty off flavors. It also darkens, and starts to smoke pre-
maturely. ALA cannot be removed from the rest of the oil
easily or cheaply (it makes up about 10 percent of the volume
of the oil), but hydrogenation can “fill in the blanks,” turning
the unsaturated double bonds missing hydrogens into saturated
single bonds with a full complement of hydrogens, stabilizing
the oil by building in resistance to oxidation. Unfortunately,
unless the fatty acid is fully hydrogenated, with every unsatu-
rated double bond converted to a saturated one, trans fats are
created.
The use of nickel as a catalyst during the hydrogenation
process promotes the conversion of the natural cis configura-
tion (kinked, with both of the attached hydrogens on the
same side of the carbon backbone) of unsaturated fatty acids
to the trans form (straight, with the two attached hydrogens
on opposite sides of the carbon backbone), allowing for closer
packing of the fatty acids and a more solid consistency of the
fat. Changing the nickel catalyst to another metal, such as
gold, reduces the formation of trans fatty acids during hydro-
genation. This costly solution is not economically practical.
Researchers at the U.S. Department of Agriculture (USDA)
developed a method that increases the pressure and temper-
ature of the hydrogenation process. Using soybean oil, they
created a product containing 17 percent trans fatty acids by
weight, compared to the usual process, which contains
40 percent. When blended with liquid oil to make margarine,
the level of trans fatty acids falls below the level that must be
declared on the nutrition label.2
Many other solutions have been developed to reduce the
level of trans fatty acids. Low‐linolenic soybean varieties have
been developed and are being processed into soybean oil that
does not need to be hydrogenated to increase stability. This
oil is stable enough to be used in frying applications because
the unstable linolenic acid levels are reduced. Another tech-
nique is to completely hydrogenate an oil to change the
18‐carbon unsaturated fatty acids (oleic, linoleic, and lino-
lenic fatty acids) into stearic acid, a fully saturated 18‐carbon
fatty acid. This fully saturated, solid fat can then be blended
with oil to create the texture desired.
The use of palm or coconut oil in place of hydrogenated oil
is another option. These are naturally trans fat–free vegetable
oils that are solid or semisolid at room temperature without
the need for hydrogenation. They can be fractionated to pro-
duce a range of products with many physical properties
appropriate for baking applications. The downside of these
tropical oils is that although they are trans fat– and choles-
terol‐free, they are saturated and pose many of the same
health concerns as saturated animal fats.
Nutrition and Essential
Fatty Acids (EFAs)
Fats and oils are an abundant source of energy. They provide
9 calories per gram compared to 4 calories per gram from car-
bohydrates and proteins. While the Western diet is typically
not deficient in calories (in fact, quite the opposite), this is
not true of other parts of the world. As with any diet, a bal-
ance of calories from carbohydrates, protein, and fats and oils
must be maintained for weight control and overall health.
A minimum of 6 to 15 percent of total calories from fat is
believed necessary in order to promote:
• Proper central nervous system balance
• Cell membrane formation and function
• Proper immune response
• Proper skin, hair, and nail function
• Proper hormonal function
Fats and oils are also carriers for the fat‐soluble vitamins
A, D, E, and K. These are essential for proper body function.
Because they cannot be produced by the body, dietary
sources are essential. However, without fats and oils in the
diet, these vitamins cannot be absorbed; they would pass
right through the digestive tract.
The nutritional impact of fats and oils may outweigh other
desirable functions that they bring to food. All fats and oils
are made up of a combination of saturated, monounsatu-
rated, and polyunsaturated fatty acids. Even fats that are cate-
gorized as being saturated or unsaturated are never 100 percent
of either. Lard, for instance, has more unsaturated fatty acids
(60 percent) than saturated ones (40 percent)! And with
90 percent saturated fatty acids, coconut oil is even more sat-
urated than lard! The percentage of each of these varies with
the source of the fat or oil and the types of refining or other
processing it is subjected to. Each type of fat is identified
based on the level of each of these components. Animal fats
contain cholesterol and (except for fish oils) predominantly
saturated fats, both of which are implicated as causative
agents of human disease. In addition, fats that are partially
hydrogenated or deodorized also contain trans fats.
Others fats contain essential fatty acids (EFAs), which are
necessary for good health. EFAs cannot be made in sufficient
quantity by the human body and therefore must be consumed
via the diet. The two EFAs for humans are linoleic acid (LA)
(an omega‐6 fatty acid) and alpha‐linolenic acid (ALA) (an
omega‐3 fatty acid). These molecules are identical in carbon
chain length (18 carbons each); they differ in the number and
c09.indd 173 1/14/2016 7:26:51 AM
174 • CULINOLOGY®
location of the double bonds. LA has two, one at the #6 carbon
(hence omega‐6 fatty acid) and one at the #9 carbon, while
ALA has three, one each at the #3 carbon (omega‐3 fatty acid)
and the #6 and #9 carbons. It is believed that these EFAs are
involved in regulating inflammation and ALA in particular
may decrease the risk of diseases including heart disease, can-
cer, and arthritis. Essential fatty acids are also important for
cognitive function and maintenance of good vision. That said,
the two EFAs are dependent on each other and must be pres-
ent in the diet in the right ratio in order to maintain optimum
health. In an optimum diet, the ratio is no more than 4:1
omega‐6:omega‐3. In the typical American diet, however, the
ratio is greater than 10:1 omega‐6:omega‐3. Unfortunately,
many of the benefits of the omega‐3 fatty acids are inhibited
when the level of omega‐6 fatty acids is high. This is one rea-
son why nutritionists strongly recommend taking fish oil sup-
plements, which are especially high in omega‐3 fatty acids,
and reducing the consumption of vegetable oils such as corn,
sunflower and cottonseed, all of which contain extremely high
levels of LA and virtually no ALA.
ALA is particularly important because it is the source in
the body of two other near‐essential omega‐3 fatty acids
credited with most of the good health attributes: DHA (doco-
sahexaenoic acid), a 22‐carbon fatty acid with six double
bonds, and EPA (eicosapentaenoic acid), a 20‐carbon fatty
acid with five double bonds. They are near‐essential because
they can be synthesized in the body from alpha‐linolenic
acid. However, this inefficient process does not yield suffi-
cient quantities of DHA and EPA for optimum health.
Therefore, it is commonly accepted that additional dietary
sources are needed. The best dietary source of EPA and DHA
is fatty fish such as salmon, herring, sardines, mackerel, hali-
but, and, to a lesser extent, tuna. While vegetable sources
(flaxseed oil, hemp oil) of these omega‐3s do exist, absorp-
tion of them by the body and accumulation in the tissues is
much greater from animal sources. In 2004, the FDA
approved the following qualified health claim: “Supportive
but not conclusive research shows that consumption of EPA
and DHA [omega‐3] fatty acids may reduce the risk of coro-
nary heart disease.”
Cholesterol and Saturated Fat
Cholesterol is an essential structural component of all mam-
malian cell membranes (makes up approximately 20% of the
membrane). It is required to establish proper membrane per-
meability and fluidity and is thus manufactured by every cell.
Within the cell membrane, cholesterol also functions in
intracellular transport, cell signaling, and nerve conduction.
Cholesterol is essential for all animal life.
For a man of about 150 pounds, the body synthesizes
about 1 gram of cholesterol per day, and total body content is
about 35 g (a little over an ounce), primarily located within all
the membranes of all the cells of the body.
Typical dietary intake of additional cholesterol in the
United States is 200–300 mg per day. All foods containing ani-
mal fat contain cholesterol to varying extents. However, most
ingested cholesterol is poorly absorbed, and the body
compensates for any absorption of additional cholesterol by
reducing internal cholesterol synthesis. For these reasons,
cholesterol intake in food has little, if any, effect on total body
cholesterol content, or concentrations of cholesterol in the blood.
Instead, it is the intake of excessive amounts of saturated fats
that can increase blood cholesterol levels.
Since cholesterol is insoluble in blood, it is transported in
the circulatory system within lipoproteins. There are several
types of lipoproteins in blood; the most well known are
low‐density lipoprotein (LDL) and high‐density lipoprotein
(HDL). Higher concentrations of LDL combined with lower
concentrations of HDL are strongly associated with cardio-
vascular disease because this combination promotes ather-
osclerosis (so‐called “hardening of the arteries”). This disease
leads to heart attack, stroke, and peripheral vascular disease.
Since higher blood LDL contributes to this process more
than the HDL particles, LDL particles are often termed bad
cholesterol.
On the other hand, high concentrations of HDL, which
can remove cholesterol from cells and atheroma (arterial
plaques) and transport cholesterol back to the liver for dis-
posal, offer protection and are sometimes referred to as good
cholesterol. Having large numbers of HDL particles correlates
with better health outcomes.
Based on Mayo Clinic recommendations, the total blood
cholesterol level should be: <200 mg/dL for normal blood
cholesterol; 200–239 mg/dL is borderline‐high, and >240 mg/dL
is high cholesterol.
Most people should aim for an LDL level below 130 mg/dL.
If you have other risk factors for heart disease, your target
LDL may be below 100 mg/dL. If you’re at very high risk of
heart disease, you may need to aim for an LDL level below
70 mg/dL. In general, the lower your LDL cholesterol level is,
the better. There is no evidence that very low LDL cholesterol
levels are harmful.
Regarding HDL, the recommended level should be above
50 mg/dL, with higher concentrations providing even better
protection. Levels less than 40 mg/dL are a danger signal.
Several human studies have shown a correlation
between trans fat consumption and elevated low‐density
lipoprotein (LDL) bad cholesterol and lowered high‐
density lipoprotein (HDL) good cholesterol. In that way, it
behaves like saturated fats.
Both saturated fats and trans fats actually raise total
serum cholesterol levels, and are especially concerning as
they also lower the level of “good” cholesterol (HDL) while
raising the level of “bad” cholesterol (LDL).
Polyunsaturated fat lowers total cholesterol, a good thing,
and does lower the LDL levels. However, it also lowers the
level of HDL at the same time.
Monounsaturated fat (as found in olives and olive oil, avo-
cados, almonds, and peanuts and peanut butter) is neutral in
respect to total cholesterol, but instead raises the good
cholesterol and lowers bad cholesterol at the same time. This
has been suggested as a key reason for the healthiness of the
so‐called Mediterranean diet.
c09.indd 174 1/14/2016 7:26:51 AM
CHAPTER 9 • Lipid-Based Foods • 175
Functions of Fats
and Oils in Food
and Cooking
Fats and oils are added to foods for a variety of reasons. They
provide a richness of flavor, a creamy mouthfeel, and even a
flaky texture. They also serve as a cooking medium and pro-
vide a feeling of satiety, or fullness.
Lubricity
Fats and oils can be used as lubricants during machining,
processing, and mastication (chewing) and swallowing of
foods—think about the difference in chewing and swallowing
a juicy hamburger prepared with 80:20 ground beef (80%
lean:20% fat) vs. one made with lean or extra lean. Have you
ever thought about what allows a loaf of bread to be sliced?
The presence of just a small amount of oil or fat in bread gets
into the gluten fibers that form during mixing and set during
baking, giving just enough lubricity for a slicing machine to
work effectively. Breads that do not typically contain fat, like
Italian and French breads, are not typically sliced.
Fats and oils act as a lubricant on the surface of a grill,
griddle, or frying pan to keep food from sticking to the sur-
face. They are also used to grease pans used for baking (muf-
fin tins, cake pans, bread pans, and so on).
Intrinsic Flavor
Each type of unprocessed fat and oil possesses a characteris-
tic flavor. The refining process removes volatiles (aromatic
molecules with low boiling points) from the oil as well as any-
thing that causes off flavors or off odors to develop. Because
of this, the characteristic flavors of processed fats or oils are
often not what you would expect. For instance, peanut oil
does not taste nutty; it tastes bland and allows the flavor of
the food to come through rather than be masked. Processed
lard does not make pie crusts taste like pork. Refined fats are
selected for processing foods transparently, leaving nothing
of their own flavor behind. On the other end of the spectrum
are fats and oils that are not typically deodorized and main-
tain their natural, or characterizing, flavors. These include
butter, bacon fat, toasted sesame oil, olive oil, and infused oils
(garlic, herb, lemon, truffle, etc.), which are chosen based on
the flavor they bring to the food. The flavor of the oil is an
important consideration that naturally depends on the type
of finished product desired.
Carrier/Promoter of Flavors
and Colors
Because deodorized fats and oils are bland, they can be an
excellent delivery medium for oil‐soluble flavors and colors.
Common flavored oil applications include:
• Air‐popped popcorn
• Croutons
• Ready‐to‐eat cereals
• Coatings for savory snacks (for seasoning adhesion)
• Topical spray oils for nuts
Many flavor compounds, herbs, and spices are fat soluble.
These can be carried into the food by being dissolved in the
fat or oil used in the food formulation. One of the conse-
quences of removing fat from food is the loss of the carrier for
fat‐soluble flavor compounds. In addition, the process of sau-
téing certain foods in oil, such as onions and garlic, helps
develop and smooth out their flavor, appearance, and aroma.
Similar flavor development takes place when meats are
seared in hot fat.
Several food colorants are also fat‐soluble and require oils
not just as a carrier but also as a diluent. Natural examples
include annatto and the carotenes, and synthetics including
the lake pigments made from FD&C dyes. In addition, fat‐
soluble colors are responsible for the yellow pigment in
chicken skin and the orange pigment in salmon flesh.
Mouthfeel
Mouthfeel is related to the initial textural perception felt as
the food enters the mouth, and it continues through chewing
and swallowing. The flavors remaining in the mouth also
impact the overall mouthfeel. The presence (or absence) of
fats and oils in the food greatly influences mouthfeel sensa-
tions. Decisions about the amount and type of fat (solid or
liquid) greatly affect every aspect of taste perception.
Consider two examples: high‐quality chocolate and vegetable
shortening. The chocolate contains fat (cocoa butter) that
has a melting point very close to that of our body tempera-
ture. Eating high‐quality chocolate conveys a creamy, melt‐
in‐the‐mouth experience. However, the contrary is true of
shortening, which is often made from fats with a much higher
melting point. That unpleasant, waxy, mouth‐coating feeling
you get from eating an icing made only of shortening is
another sort of mouthfeel, in this case a negative one.
Emulsions
An emulsion is a liquid dispersed in another liquid with
which it is immiscible—that is, two liquids that cannot form
a homogenous mixture without mechanical or chemical
assistance (examples include milk, oil and vinegar, cake
batter, mayonnaise, and ice cream). The two parts of an
emulsion are the continuous (or external) phase and the
discontinuous (or dispersed or internal) phase. For example,
in butter (a water‐in‐oil emulsion), the fat is the continuous
phase and the water is the dispersed phase. In cream (an oil‐
in‐water emulsion), the water is the continuous phase and
the fat is the dispersed phase.
Fats are incorporated into many water‐based foods, like
salad dressings and sauces. Because oil and water are immis-
cible, they separate unless physical energy or chemical emul-
sification is used to prevent it. Emulsifiers are compounds
with both fat‐soluble and water‐soluble components. The
fat‐soluble (lipophilic) end of the emulsifier binds to oil, while
c09.indd 175 1/14/2016 7:26:51 AM
176 • CULINOLOGY®
the water‐soluble (hydrophilic) end binds to water. The result
is that when the two liquids are mixed, the emulsifier stabi-
lizes the emulsion, preventing fat globules from coalescing.
Mayonnaise, a water‐in‐oil emulsion, exploits the naturally
occurring emulsifier in the egg yolk (lecithin). This results in
a permanent emulsion.
A temporary emulsion results when energy alone is used
to bring the two phases together—for example, shaking a
mixture of oil and vinegar to make vinaigrette. The two
phases separate when the energy is no longer forcing the
compounds together.
A semi‐permanent emulsion is one where the two com-
ponents stay together for a while but eventually separate.
Dressings containing a viscous component such as honey or
simple syrup remain intact because the increased viscosity of
the emulsion inhibits, but does not prevent, oil droplets from
coalescing over time.
Common natural emulsifiers include lecithin, a phospho-
lipid naturally present in egg yolk and soybeans, and mono‐
and diglycerides derived from an assortment of lipids.
Powdered herbs and spices such as mustard, paprika, and
finely ground black pepper also provide some emulsification
by inhibiting coalescence of the fat globules. Synthetic
emulsifiers include propylene glycol and Tween, a family of
polysorbate emulsifiers.
Heat Transfer
Of the three macronutrients in the diet—proteins, carbohy-
drates, and fats—only fats can be used as a cooking medium.
That is because they are excellent at heat transfer. Fats are
used in deep‐fat frying, wok stir‐frying, pan‐frying, sautéing,
nut‐roasting, and baking. Fats and oils absorb large amounts
of heat before changing their physical state, which makes
them very effective at transferring heat to food. As soon as
water reaches 212°F it turns to steam. Oils withstand tem-
peratures in excess of 400°F.
Consider fried chicken. After the raw chicken is seasoned
and dredged in flour, it is a soft, pliable, unappetizing lump.
When it is put in the fryer and heat is transferred to it, color
and flavor begin to develop. The hot fat heats the moisture in
the chicken and breading. As the moisture (in the form of
steam) escapes the chicken, the breading begins to dehydrate
and the meat begins to cook. Dehydration of the surface and
fat absorption during frying impart desirable taste, texture,
mouthfeel and crust development to the coating. The high
heat in the presence of proteins and reducing sugars in the
breading promote Maillard browning and fried flavor develop-
ment. As heat moves into the interior of the chicken via con-
duction, the raw flesh goes from soft and flaccid to cooked and
succulent. What comes out of the fryer is a masterpiece of culi-
nary delight that cannot be duplicated in a pot of boing water!
Baking
One of fat’s primary uses in baked goods is to tenderize. Fat
coats flour particles to inhibit water from hydrating the
gluten‐forming proteins, glutenin and gliadin, thus reducing
the firm and chewy texture that gluten provides. Shortening
gets its name from its ability to shorten gluten strands. The
more fat in a product, the more tender it is.
For example, lean bread dough, like a French baguette, is
chewy due to gluten formation. Compare this to brioche,
another traditional French bread, which has a fairly rich
dough and therefore a softer texture. The type of fat used and
how it is incorporated also affect the texture of the final
product. Compare the textures of a biscuit (fat is cut in), puff
pastry (fat is layered in), and brioche (fat is thoroughly
incorporated in the dough).
Solid fat is usually used in shortened cakes (such as pound
cake). The first step is to cream the fat and sugar. The angular
sugar crystals form pockets in the fat in which air is then
entrapped. The hardness of the fat determines how many and
how fine the air pockets are. It is easier to incorporate the sugar
into shortening than butter because shortening is a plastic fat,
and the result is a cake with fine, small air pockets. Butter has
a narrow melting range, making it more difficult to incorpo-
rate the sugar because the fat quickly goes from being too
hard to trap air cells effectively to being too soft. Butter pro-
vides a more desirable flavor though, so the two fats are often
used together for optimum texture and flavor. Shortening
also contains emulsifiers that help incorporate liquid ingredi-
ents and may result in a finer texture.
In addition to tenderness, many baked products also need
flakiness. Examples include pastries, croissants, phyllo
dough, pie crust, and biscuits. Flakiness is achieved by cut-
ting solid fat into flour to maintain discrete fat globules. In
the case of croissants and breakfast Danish, the fat is literally
rolled into the dough in sheets, creating layers of fat and
dough. The dough is then folded back on itself, chilled,
and sheeted many times until there are literally hundreds of
FIGURE 9.9 Dessert tray.
Source: Cargill, Incorporated.
c09.indd 176 1/14/2016 7:27:04 AM
CHAPTER 9 • Lipid-Based Foods • 177
interleaved dough and fat layers. When this layered pastry is
baked, the fat melts and the moisture turns to steam, provid-
ing leavening in the baked product, while all of the sheeting
and folding provides the flaky layers. The more fat and the
more care taken incorporating it into the product, the flakier
the product is. The key is to use a solid fat. A liquid oil coats
too many flour particles and results in a soft product rather
than a flaky one.
Frying
Frying is one of the most important uses for fats. The crisp
texture of fried foods is highly desirable and difficult to repli-
cate by other cooking methods. Fat absorbs large amounts of
heat and transfers it to food. Typical frying temperatures are
between 350 and 400°F (177 and 204°C). Compare this to
water, which can reach only 212°F (100°C) at normal pressure
unless other solutes (such as salt or sugar) are added to it.
Food can be fried in different amounts of fat, as in deep‐fat
frying (submerged food), pan‐frying (medium amount of fat),
and sautéing (small amount). When food is placed in hot fat,
its surface dries immediately as the water turns to steam. This
contributes to the crisp texture of fried foods and is also
responsible for what looks like the boiling of the oil around the
food item as steam bubbles up through the fat. The interior of
the food is cooked by conduction, as heat is transferred from
the heated surface to the cooler interior. One of the challenges
in frying food is cooking its interior without overcooking the
exterior. That is one reason why deep‐frying is better suited
for smaller pieces of food rather than large chunks (the recent
fad of deep‐frying whole turkeys notwithstanding). French
fries use a two‐step method, first a blanching step, to cook the
interior of the food at a lower temperature; then a final cook-
ing at a higher temperature, to develop the crisp texture.
While everyone likes the texture of fried food, we do not
like it to look, feel, or taste greasy. The amount of fat absorbed
depends on several factors: the length of time the food
FIGURE 9.10 Approximate smoke points
of common cooking oils.
Fat
Smoke
Point °F
Smoke
Point °C
Unrefined canola oil 225°F 107°C
Unrefined flaxseed oil 225°F 107°C
Unrefined safflower oil 225°F 107°C
Unrefined sunflower oil 225°F 107°C
Unrefined corn oil 320°F 160°C
Unrefined high‐oleic sunflower oil 320°F 160°C
Extra virgin olive oil 320°F 160°C
Unrefined peanut oil 320°F 160°C
Semirefined safflower oil 320°F 160°C
(continued)
Fat
Smoke
Point °F
Smoke
Point °C
Unrefined soy oil 320°F 160°C
Unrefined walnut oil 320°F 160°C
Hemp seed oil 330°F 165°C
Butter 350°F 177°C
Semirefined canola oil 350°F 177°C
Coconut oil 350°F 177°C
Unrefined sesame oil 350°F 177°C
Semirefined soy oil 350°F 177°C
Vegetable shortening 360°F 182°C
Lard 370°F 188°C
Macadamia nut oil 390°F 199°C
Refined canola oil 400°F 204°C
Semirefined walnut oil 400°F 204°C
High-quality (low-acidity)
extra virgin olive oil 405°F 207°C
Sesame oil 410°F 210°C
Cottonseed oil 420°F 216°C
Grapeseed oil 420°F 216°C
Virgin olive oil 420°F 216°C
Almond oil 420°F 216°C
Hazelnut oil 430°F 221°C
Peanut oil 440°F 227°C
Sunflower oil 440°F 227°C
Refined corn oil 450°F 232°C
Palm oil 450°F 232°C
Palm kernel oil 450°F 232°C
Refined high‐oleic sunflower oil 450°F 232°C
Refined peanut oil 450°F 232°C
Refined safflower oil 450°F 232°C
Semirefined sesame oil 450°F 232°C
Refined soy oil 450°F 232°C
Semirefined sunflower oil 450°F 232°C
Olive pomace oil 460°F 238°C
Extra light olive oil 468°F 242°C
Soybean oil 495°F 257°C
Safflower oil 510°F 266°C
Avocado oil 520°F 271°C
Source: Courtesy of Cooking for Engineers (www.cookingforengineers
.com).
FIGURE 9.10 (continued)
c09.indd 177 1/14/2016 7:27:04 AM
http://www.cookingforengineers.com
http://www.cookingforengineers.com
178 • CULINOLOGY®
remains in contact with the oil, the age of the oil, the tem-
perature of the oil, the amount of food in relation to the size
of the fryer, and the ingredients in the food. The food should
be cooked at the highest temperature possible for the short-
est time to achieve a product that is done on the inside and
not too brown on the outside. Foods high in fat and sugar
increase fat absorption, while foods high in protein prevent
the absorption of fat. Doughnuts containing a high percent-
age of sugar and fat absorb more fat while frying.3
The desirable crisp texture of fried foods does not last long
because the remaining moisture in the interior of the food
eventually migrates to the drier surface. To make the most of
the crisp texture, fried food should be consumed as soon as
possible after cooking.
The choice of a fat for frying depends on the desired out-
come. Butter contributes a very desirable flavor. However, it
is hard to fry with because of its water content (15 percent)
and the presence of milk solids, which brown and burn at
lower temperatures than those required for optimum frying.
To use butter as a frying fat, it must first be clarified—that is,
its water and milk solids must be removed from the butterfat.
Even then, butterfat has a fairly low smoke point, so it can be
used only in quick frying applications, such as sautéing. (Note:
In India, clarification of butter is a common practice because
removal of water and milk solids extends the unrefrigerated
shelf life of the finished product, which is called ghee.)
Beef tallow and pork fat (lard) also impart a desirable fla-
vor to fried food, but both contain cholesterol, which many
consumers find undesirable. As a result, for many years fast
food companies had replaced animal fats in their fryers with
tallow‐flavored partially hydrogenated vegetable oils. With
the discovery that trans fats are present in partially hydro-
genated fryer oils, there has been a general movement to
other, non‐partially hydrogenated oils that have been created
specifically for frying using technologies (such as interesteri-
fication) that do not create trans fats.
Most fats and oils can be heated to fairly high tempera-
tures (in excess of 300°F [149°C]) before they begin to break
down. However, the bonds that attach the fatty acids to the
glycerol backbone begin to break with continual heating,
resulting in smoke. The temperature at which this occurs is
the smoke point, which is different for each fat or oil. The
level of saturation is the main factor influencing smoke point.
The more unsaturated a fat, the higher its smoke point. For
example, heavily saturated lard begins to smoke around
375°F (190°C), but unsaturated peanut oil doesn’t begin to
smoke until 450°F (232°C) and safflower oil until 510°F
(265°C). If heated past the smoke point, the flash point is
reached and the fat or oil ignites.
The antioxidants BHA, BHT, and TBHQ are used in fats to
extend shelf life and increase the number of times the fat can
be reused. This is an important feature because fryer fat is an
expensive ingredient, and it is often reused. While fryer fats
won’t last forever, effective frying life can be maximized by
regular filtering to remove burned particulates, avoiding the
introduction of water and salt, and not overloading the fryer.
Rancidity
One of the major problems associated with fats and oils is
rancidity. In addition to the unpleasant flavor and aroma
associated with rancid fat (described as paint‐ or varnish‐
like), it loses heat transfer efficiency, darkens considerably,
and becomes viscous (thickens). Once fats become rancid,
neither the fats themselves nor the foods made with them
can be salvaged; they must be discarded. The two major types
of rancidity are hydrolytic and oxidative.
In hydrolytic rancidity, the fatty acids are removed from
the glycerol backbone by the addition of water. This reaction
is caused by extended high-heat exposure in the presence of
water (as in deep frying, where water is constantly expelled
from frying foods) or by the presence of lipolytic enzymes.
Glycerol, the three‐carbon molecule that serves as the
backbone of fatty acids, decomposes during extended high‐
temperature exposure and becomes acrolein, a volatile and
irritating compound with a piercing, disagreeable, acrid smell
of burning grease.
Another consequence of overheating is polymerization.
As heating continues, the free fatty acids link together into
long chains, leading to formation of a gummy dark residue on
pots and pans, and an oil that is more viscous and prone to
foaming. Additionally, the oil loses heat transfer efficiency.
Polymerization thus leads to increased fat absorption, result-
ing in greasy fried foods with darker color and bitter flavor.
This is why frying oil can only be used a limited number of
times before the onset of hydrolytic rancidity and explains
the gummy buildup inside a fryer. Regular thorough cleaning
of the fryer and filtering of the oil can help extend the life of
the oil, but ultimately every oil will break down past the point
of usefulness.
Oxidative rancidity is even more of a problem because it
can occur without the addition of heat (although heating
exacerbates the reaction). As the term implies, oxygen is
involved. It may also be referred to as auto‐oxidation because
once started, it is self‐perpetuating. The three steps in oxida-
tive rancidity are initiation, propagation, and termination.
In the initiation stage, a free radical is created. (A free
radical is any atom or molecule with a single unpaired elec-
tron. It is highly unstable and therefore highly reactive.) One
of the hydrogen atoms on the fatty acid chain breaks away
from an unsaturated (double‐bonded) carbon atom. Because
free radical formation always involves a hydrogen located at
an unsaturated double bond, polyunsaturated fatty acids are
the most prone to oxidative rancidity. Common causes
are exposure to ultraviolet light (sunlight), table salt, or min-
erals such as copper or iron. With the hydrogen removed, the
carbon with only three bonds becomes an unstable, highly
reactive free radical that readily binds with oxygen (becomes
oxidized) to become a peroxide free radical. This is called the
propagation stage. The peroxide free radical “attacks” and
removes another hydrogen from an adjacent fatty acid,
making a hydroperoxide. This creates another peroxide free
radical—and the reaction continues until all of the double
bonds on the molecule are transformed and the fatty acids
c09.indd 178 1/14/2016 7:27:04 AM
CHAPTER 9 • Lipid-Based Foods • 179
are broken down into highly aromatic and malodorous
smaller compounds (acids, aldehydes, ketones, and alcohols),
resulting in rancid off odors and flavors. This is the termina-
tion stage.
The level of auto‐oxidation in an oil is measurable before
obvious signs of rancidity are present (to predict its working
lifespan) by a number of chemical tests, including peroxide
value, which measures the formation of peroxides, and the
thiobarbituric acid (TBA) test, which measures the formation
of malonic dialdehyde, an end product of oxidation (but not a
cause of off flavors). Gas‐liquid chromatography (GLC) and
high‐pressure liquid chromatography (HPLC) are also used to
follow the formation of specific volatile compounds that are
a more direct measure of lipid oxidation, but these are rarely
used outside of the laboratory.
The most common way to prevent oxidative rancidity is to
reduce exposure of the lipid to oxygen. Putting an inert gas
such as nitrogen in the headspace of bottled oils works well to
prevent oxidation from occurring in an unopened bottle of oil.
Keeping oil away from reactive metals is also important (this
is why deep fryers are made of inert stainless steel). Chelating
agents such as EDTA (ethylenediaminetetraacetic acid) are
often used to bind and remove iron or copper from oils. The
use of antioxidants also delays lipid oxidation. Common natu-
ral antioxidants used with oils include vitamins C (ascorbate)
and E (tocopherols). Synthetic antioxidants include butylated
hydroxytoluene (BHT), butylated hydroxyanisole (BHA), and
tertiary butylated hydroquinone (TBHQ). These work by
donating a hydrogen atom of their own to the peroxide free
radical carbon, stabilizing it so it doesn’t steal one from its
neighboring carbon on the chain, thus stopping the propaga-
tion of free radicals. In effect, the antioxidant becomes oxi-
dized instead of the fatty acid. Eventually the antioxidant loses
its ability to donate hydrogen, but the oxidation process is
delayed and the shelf life of the oil extended.
Keeping the oil in a cool, dark environment also increases
shelf life by limiting its exposure to heat and ultraviolet light.
Furthermore, reducing exposure of frying fats to table salt by
delaying the seasoning of fried products until after they come
out of the fryer is highly recommended.
Since unsaturated fatty acids are most susceptible to oxi-
dative rancidity, removal of double bonds by hydrogenation is
also done. Although this process results in a more stable oil, it
also creates trans fatty acids, which have negative health
consequences.
Conclusion
Fats and oils are important for both their nutritional and
functional characteristics. They are also components of
many foods, imparting qualities hard to replicate with other
ingredients. The role of fats and oils in the diet continues to
be debated by nutritionists and food scientists. With new
technology, new lipid ingredients will be available to future
chefs and product developers. These developments will
assure the continued important role fats and oils play in the
food supply.
1T. Carr and E. Jesch, “Food Components That Reduce Cholesterol Absorption,”
Advances in Food and Nutrition Research 51 (2006):165–204.
2F. Eller, “Preparation of Spread Oils Meeting U.S. Food and Drug
Administration Labeling Requirements for Trans Fatty Acids via Pressure‐
Controlled Hydrogenation,” Journal of Agricultural and Food Chemistry 53,
no. 15 (2005):5982–5984.
3M. Bennion and B. Scheule, “Fats, Frying, and Emulsions,” in Introductory
Foods, 11th ed. (Columbus, OH: Prentice Hall, 2000), 395.
c09.indd 179 1/14/2016 7:27:04 AM
10
180 • CULINOLOGY®
Egg‐ and Milk‐Based
Foods
Lead Author: John U. McGregor, Ph.D. , Professor, Food, Nutrition, and Packaging Sciences
Department , Clemson University
Julie K. Northcutt , Ph.D. , Professor, Food, Nutrition, and Packaging Sciences Department ,
Clemson University
Michelle Parisi , Ph.D. , Food, Nutrition, and Packaging Sciences Department , Clemson University
Tonya C. Schoenfuss, Ph.D. , Associate Professor, Department of Food Science and Nutrition ,
University of Minnesota
Christian Thormose , Chef/Food Production Manager , ARAMARK
Working with Dairy
and Egg‐Based
Products
Whether you characterize yourself as a chef, food scientist, or
Culinology® professional, the functional properties of dairy
and egg‐based products offer exceptional opportunities as
components and ingredients in formulated foods. Classically
trained chefs have long considered these products funda-
mental elements in dishes that deliver a wide range of eating
experiences, from breakfast to dinner, from snacks to bever-
ages to entrées to desserts. Product developers who focus on
delivering innovative, nutritious, and wholesome foods often
employ egg and dairy components to achieve myriad func-
tions in the manufacture of formulated foods. The new gen-
eration of Culinology® professionals are combining scientific
knowledge and culinary training to use eggs and dairy‐based
products in the creation and manufacture of new products
and applications that are defining the future of food.
Eggs
Composition of Eggs and
Egg Components
Eggs comprise a shell, yolk, latebra (white yolk), germinal disc
(small, circular white spot on the yolk where the nucleus is
and where embryos develop if the egg is fertilized), vitelline
(yolk) membrane, chalazae (spiral bands of protein that
suspend the yolk in the middle of the white), air cell, shell
membranes, chalaziferous layer (thin layer of albumen
enclosing yolk membranes), thin albumen (white), and thick
albumen (white).
Egg white ( albumen ), which constitutes 60 to 63 percent
of a fresh egg, is made up of four alternating layers of thick
and thin consistencies. It is approximately 90 percent water;
the remaining components are about 10 percent protein plus
small amounts of fat (0.03 to 0.07 percent) and carbohydrate
(0.4 to 1.3 percent). The carbohydrates of albumen are a com-
bination of free forms (usually glucose) and combined forms
( glycoproteins , which are proteins with carbohydrates
attached) containing the sugars mannose and galactose.
Albumen also contains varying levels of sulfur, potassium,
sodium, phosphorus, calcium, magnesium, iron, and the vita-
mins niacin and riboflavin. Albumen contains some dis-
solved carbon dioxide, which affects the pH of the egg. As
carbon dioxide dissipates from the egg during storage (the
shell is porous), the pH of the albumen correspondingly
increases. In a fresh egg, pH is between 7.6 and 8.5 and can
increase to 9.4 during storage of 21 days. 1
Albumen contains more than 40 proteins in addition
to the glycoproteins. Ovalbumin (54 percent), conalbumin
(12 percent), and ovomucoid (11 percent) make up almost 80
percent of the total protein found in albumen. 2 The proteins
of most interest to food product developers are ovalbumin
(foaming and coagulation), conalbumin (foam stabilization
through binding with metal ions), ovomucin (viscosity
enhancement, thickening), lysozyme (antibacterial agent),
and globulins (foaming and viscosity enhancement).
c10.indd 180 1/22/2016 1:10:44 PM
CHAPTER 10 • Egg- and Milk-Based Foods • 181
Egg yolk makes up 27.5 percent of a fresh egg. The natural
oil‐in‐water emulsion is approximately 50 percent water,
17 percent protein, and 33 percent lipid. There are four classes
of proteins in egg yolk: lipoproteins, lipid‐free globular pro-
teins, phosphoproteins, and minor proteins. More than 90
percent of the lipids in egg yolk are in the form of lipoproteins
(conjugated protein having a lipid component, either low‐
density lipoproteins [LDL] or high‐density lipoproteins
[HDL]). Egg yolk also contains varying levels of iron, phospho-
rus, calcium, manganese, iodine, copper, zinc, and the vita-
mins A, D, B12, E, choline, folate, inositol, panthothenic acid,
pyridoxine thiamin, riboflavin, and biotin. The orange‐yellow
COMPOSITION
Shell
• Outer covering of egg,
composed largely of
calcium carbonate
• May be white or brown
depending on breed of chicken
• Color does not affect egg
quality, cooking characteristics,
nutritive value, or shell thickness
Air Cell
• Pocket of air formed
at the large end of egg
• Caused by contraction
of the contents during
cooling after laying
• Increases in size as
egg ages
Shell Membranes
• Two membranes—inner
and outer shell
membranes surround
the albumen
• Provide protective
barrier against bacterial
penetration
• Air cell forms between
these two membranes
Thin Albumen (White)
• Nearest to the shell
• Spreads around thick
white of high-quality
egg
Thick Albumen (White)
• Major source of egg
riboflavin and protein
• Stands higher and
spreads less in higher-
grade eggs
• Thins and becomes
indistinguishable from
thin white in lower-
grade eggs
Yolk
• Yellow portion of egg
• Color varies with feed
of the hen, but doesn’t
indicate nutritive
content
• Major source of egg
vitamins, minerals,
and fat
Germinal Disc
Chalazae
• Twisted, cordlike strands
of egg white
• Anchor yolk in center of egg
• Prominent chalazae
indicates freshness
Vitelline (Yolk)
Membrane
• Holds yolk
contents
FIGURE 10.1 General egg structures.
Source: American Egg Board.
Thick white
Thin white
FIGURE 10.2 Thick and thin egg white.
Source: Julie Northcutt.
Membrane
Germinal disk
FIGURE 10.3 Egg membrane and germinal disk.
Source: Julie Northcutt.
c10.indd 181 1/14/2016 7:28:46 AM
182 • CULINOLOGY®
color of egg yolk comes from the carotenoid pigments lutein
and zeaxanthin. The pH of egg yolk in fresh eggs is around 6.0,
gradually increasing during storage to 6.4 to 6.9 depending on
the rate of carbon dioxide loss, length of storage, and storage
temperature.3
Lipoproteins are the primary functional component of egg
yolk and are therefore of most interest to the food product
developer. The structure of lipoproteins has regions that ori-
ent in the water phase of a food and regions that orient in the
lipid portion. This is how an oil‐in‐water emulsion, such as
mayonnaise, is formed. The proteins (with phosphorus‐ or
sugar‐containing portions) orient in the water side of a fat–
water interface, while lipid‐containing portions orient in the
fat side. The ability of the lipoproteins in egg yolk to adsorb to
the interface during whipping allow for the creation of small,
stabilized fat droplets in the water that do not separate and
rise. LDL lipoproteins are believed to be the primary contrib-
utor to egg yolk emulsification properties.
Nutrition
For only 70 calories each, eggs are rich in nutrients. They con-
tain, in varying amounts, almost every essential vitamin and
mineral needed by humans as well as several other beneficial
food components. Egg protein is the standard by which other
protein sources are measured. Each egg provides 6 g of high‐
quality, complete protein—that is, containing all of the
essential amino acids required in the human diet for optimum
health. This makes up 34 percent of the total calories in one
egg. Essentially all of the protein in an egg lies in the white
portion, which is used in myriad food functions, including
emulsifying and browning. When adding eggs to a food prod-
uct for function, nutritional benefits are a bonus.
Eggs also provide a rich source of lipids, including fatty
acids, phospholipids, triglycerides, and cholesterol. Lipids
function in the body to store energy, maintain cell membrane
integrity, regulate body temperature, insulate and cushion
internal organs, and produce hormones. Nutritionally, one
large egg contains about 5 g total fat and 1.5 g saturated fat.
Because egg yolks contain some saturated fat and cholesterol
(one large egg has about 186 milligrams of cholesterol),
scientists and consumers have been concerned about the rela-
tionship between egg consumption and risk of heart disease.
However, scientists recently concluded that the health bene-
fits4 of the protein and micronutrient content of eggs greatly
outweigh these concerns.5 As a result, recommendations for
limiting the number of egg yolks consumed each week were
eliminated from the American Heart Association’s Dietary
Guidelines for Americans. The latest edition (2010) states:
“[E]vidence suggests that one egg (i.e., egg yolk) per day does
not result in increased blood cholesterol levels, nor does it
increase the risk of cardiovascular disease in healthy people.”6
Lipid‐related trends in the egg industry further support egg
consumption with the development of specialty eggs like those
enriched with omega‐3 fatty acids. Consumption of these eggs
decreases blood cholesterol levels, a risk factor for the develop-
ment of heart disease.7 They are produced by supplementing
the feed of egg‐laying hens with fish oil or flaxseed (rich sources
of omega‐3s), which results in a higher concentration of
omega‐3s in the eggs. These specialty eggs are considered a
functional food (one that provides a health benefit beyond
meeting basic nutritional needs) because the fatty acid
composition actually enhances heart health. If used in mixed
dishes and baked products, these eggs improve the overall
nutritional value of these food items.
Proteins
Lipid
Water
FIGURE 10.4 Example of denatured and globular
proteins at the fat–water interface.
Source: Tonya Schoenfuss.
Source: Adapted from the American Egg Board.
FIGURE 10.5 Product composition of egg ingredients.
Product Moisture Protein Lipid Carbohydrate Ash
%
Whole egg 75.2 12.0 9.7 2.2 0.8
Yolk 56.8 15.3 23.0 3.6 1.4
Sugared yolk 51.2 13.9 20.8 13.0 1.1
Salted yolk 50.9 14.1 20.9 3.8 10.4
Salted whole egg 67.8 11.0 9.2 1.7 10.3
White 89.0 9.3 0.1 1.3 0.4
Dried whole egg 3.7 48.4 39.2 5.4 3.4
Dried yolk 2.7 33.7 52.9 7.3 3.3
Dried stabilized white 6.5 84.6 0.4 4.8 3.6
c10.indd 182 1/14/2016 7:28:46 AM
CHAPTER 10 • Egg- and Milk-Based Foods • 183
Another way to manipulate the nutritional makeup of a
food product using eggs is to utilize the egg whites only. This
reduces the fat content almost entirely. In baked products, for
instance, two egg whites can be substituted for one whole egg
with relatively small variation in the product’s quality while
providing big changes in its nutritional makeup.
Omitting the yolks, however, also omits valuable micro-
nutrients. Egg yolks are notably high in fat‐soluble vitamins
A and D, as well as lesser amounts of E and K. Vitamin A
(retinol) functions in maintaining normal vision, cell growth,
reproduction, and immunity. Beta‐carotene, a carotenoid
that is converted to vitamin A in the body, is also found in
the egg yolk. Beta‐carotene shows promise as a cancer‐fight-
ing agent. Vitamin D works closely with calcium and phos-
phorus (also found in eggs) to maintain blood calcium levels
and bone health. It is also related to neurological develop-
ment and is being evaluated as a treatment for depression
and multiple sclerosis.8 Deficiencies in these vitamins can
lead to a variety of physiological conditions, including night
blindness and lack of epithelial cell repair (vitamin A), as
well as an increased risk of bone disease, such as rickets in
children and osteoporosis in adults, and prostate cancer in
men (vitamin D).
Water‐soluble vitamins niacin, thiamine, riboflavin, pan-
thothenic acid, folate, B
6
, B
12
, biotin, and choline are found in
egg whites, as are minerals such as zinc, copper, and manga-
nese. The vitamins function mainly as coenzymes while the
minerals act as enzyme cofactors, both of which work with
enzymes in the body to catalyze (speed) chemical reactions
like those needed for carbohydrate, protein, and fat metabo-
lism. Deficiencies in these vitamins and minerals can result
in abnormal nervous system function, skin conditions, and
impaired cell repair. Since neither vitamins nor minerals are
synthesized in the body, they must be acquired from the
foods we eat, and eggs are an excellent source of both.
Additionally, a deficiency in choline (generally grouped in
with the B‐complex vitamins) may lead to inadequate fetal
and infant brain and neurological development. This suggests
an increased need for choline in the diet of pregnant and
lactating women. Eggs contain significant amounts of choline;
one egg per day provides sufficient amounts of choline to
meet daily recommendations for this high‐risk population.
Iron is also worth mentioning in the discussion of egg
nutrition. It is important for blood health and is a compo-
nent of hemoglobin, which transports oxygen through the
blood and delivers it to cells. A lack of iron results in low
blood oxygen levels and, if the condition persists, iron‐
deficiency anemia. Symptoms of anemia are directly related
to the lack of oxygen available for cellular metabolic
processes and cause fatigue and decreased cognitive
function. Additionally, the heart must work much harder to
pump oxygen‐deficient blood through the body, resulting in
irregular heart rhythms or heart enlargement, which can
ultimately lead to heart failure and death. Women consist-
ently do not consume enough iron to meet requirements for
the prevention of anemia, making iron‐rich food sources an
important aspect of their nutrition in particular. While meat,
poultry, and fish are excellent sources of iron, eggs are an
inexpensive alternative.
The Value of Egg
Products in Food
Product Development
Egg ingredients can be sourced as refrigerated liquid, frozen
liquid, dried, and specialty products. Refrigerated liquid
products include whole eggs, whites, yolks, sugared egg
yolks, salted whole eggs or yolks, scrambled egg mix, cooked
scrambled eggs, and extended–shelf life whole eggs, whites,
or scrambled egg mix. Frozen liquid egg products include
whole eggs, whites, yolks, scrambled egg mix, salted whole
egg or yolks, sugared egg yolks, whole eggs and yolks with
corn syrup, whole eggs with citric acid, and whole eggs with
corn syrup. Dried egg products include whole egg or yolk
solids, egg or scrambled egg mix, whole egg solids, free‐flow-
ing whole egg or yolk solids, glucose‐free stabilized whole
egg or yolk solids, and blends of whole egg or yolk with car-
bohydrates. Specialty egg products include diced hard‐
cooked peeled eggs, refrigerated whole hard‐cooked, peeled
eggs plain or pickled, frozen hard‐cooked egg rolls or long
eggs (envision a long center tube of hard‐cooked egg yolk
enwrapped in a thick layer of cooked egg white and pack-
aged like rolls of cookie dough, such that dozens of perfect
slices of hard‐boiled egg can be cut from one end of the “log”
to the other), frozen quiche mixes, frozen scrambled egg
mix, dried scrambled egg mix, and ultra‐pasteurized liquid
egg products.
USDA guidelines for pasteurizing fluid egg products
(140°F [60°C] for 3½ to 4 minutes) are designed to achieve
a 9‐D reduction in the pathogen Salmonella seftenberg.
Pasteurization at this temperature is below the albumen (egg
white) denaturation temperature range in order to keep the
eggs from curdling, but is lethal to Salmonella. Testing for
the presence of α‐amylase activity is used as a convenient
chemical method for confirming the effectiveness of egg
pasteurization. The α‐amylase is more heat resistant than
Salmonella; if enzyme activity is eliminated, that is indicative
of sufficient heating to kill Salmonella.
Egg products are typically not sourced from non‐chicken
eggs; however, in‐shell duck, quail, guinea hen, turkey, and
ostrich eggs are sold and consumed (descending order of
popularity). Very few in‐shell pheasant eggs are sold and
consumed.
Coagulation, Thickening,
Coating, and Binding
Egg products provide excellent natural thickening, coating,
and binding properties because they create stable structures
through heat coagulation of proteins. Egg whites are typically
best for this because they are virtually colorless, impart little
c10.indd 183 1/14/2016 7:28:46 AM
184 • CULINOLOGY®
flavor, and contain only trace amounts of lipids. Gelling
through protein denaturation involves the rearrangement of
the protein’s physical and chemical structure and is therefore
influenced by temperature, time, dilution, salt and sugar,
starch, and pH. All of these factors influence the protein
network—porosity, strength and placement of bonds, and
inclusions of non‐protein molecules. During heating, proteins
begin to unfold, eventually denature, and coagulate into a
protein network. Heat coagulation of egg proteins begins
around 143.6°F (62°C). At 158°F (70°C) the coagulum becomes
firm and increases with time and temperatures above 158°F
(70°C). Coagulation at these optimum temperatures makes
them an excellent adhesive ingredient for coatings in baked
and fried foods.
When egg proteins are blended with milk, water, or other
liquids, the dilution increases the physical space between pro-
tein molecules. This slows the rate of thickening and raises
the coagulation temperature and exposure time. This effect is
demonstrated in the production of egg‐ and milk‐based cus-
tards, which must be stirred and brought to 159.8°F (71°C)
before coagulation. One whole egg or two egg yolks just barely
sets 1 cup of milk. A firm gel requires the presence of more egg
protein, usually three whole eggs for each 2 cups of milk.
Other factors that affect coagulation rate:
• Homogenized whole milk conducts heat more slowly
than skim milk, so scrambled eggs or custards made
with whole milk coagulate more slowly.9
• Mineral salts such as calcium, phosphorus, and sodium
affect the electrostatic charge of egg proteins and
increase the speed and firmness of coagulation, which
is why it’s common to add a small amount of table salt
when making custard. This phenomenon also occurs
when using natural ingredient sources of mineral salts,
as when adding milk to egg‐containing mixtures such as
muffin or scone batters. Although the protein in milk
does contribute somewhat to the coagulation in custard
(less than 1 percent), milk protein is not essential for
this function. However, the calcium phosphate in milk is
essential to the coagulation of egg protein in custard.
• Sugars have an affinity for water and therefore influence
the hydrophobic interactions within and between pro-
tein molecules. When sugars are added to egg protein
systems, the coagulation temperature is increased, ena-
bling the food product developer to produce softer gels.10
• Cream fillings in pastries and desserts are meant to be
fairly thick and therefore often contain starch. Starches
can prevent protein coagulation through alteration of
the physical properties of the protein structure. In fact,
starch is sometimes added to cooked egg products to
raise the coagulation temperature and avoid curdling.
(Arrowroot is often used because it works at a lower
temperature than cornstarch and flour.) This practice
provides insurance against curdling when preparing
items such as custard or egg‐based emulsion sauces
(such as hollandaise) that will not be used immediately.
• Egg yolks contain alpha‐amylase, an enzyme that hydro-
lyzes starch molecules. Therefore, starch‐based gels
that contain egg yolks (such as custard) must be boiled
1 to 2 minutes with stirring to inactivate the enzyme.
Otherwise, they will transform the custard into liquid
overnight.11
FIGURE 10.6 Common GRAS (generally
recognized as safe by the FDA) non‐egg ingredients
added to further-processed egg products.
Ingredient Function
Salt Stabilizes proteins before
pasteurization and/or freezing
(lowers freezing point to prevent
gelling during storage to improve
shelf life).
Sugar Adds sweetness and stabilizes
proteins before freezing (lowers
freezing point to prevent gelling
during storage to improve shelf life).
Gums (guar gum,
xanthan gum,
locust bean gum)
Increases foam (whipping) stability
of whites, and increases water
retention and viscosity.
Water Improves foam (whipping) volume
of egg whites.
Yeast Added to egg whites to reduce
sugar content before spray drying
(sugars react with proteins during
drying to produce a brown color).
Hydrogen peroxide Added to egg products to lower
the pasteurization temperature.
Enzymes (glucose
oxidase)
Removes glucose before spray
drying or pasteurization to reduce
brown color formation.
Com syrup Provides sweetness, flavor and
increases the solids.
Citric acid Stabilizes color, improves water
retention and coagulation. Usually
0.1% is added.
Sodium lauryl sulfate
(surfactant)
Improves foam ability of dried eggs
whites. Usually <0.1% is added.
May also be added to hard boiled
and peeled eggs.
Triethyl citrate Improves foam (whipping) of egg
whites. Usually 0.25% is added.
Sodium benzoate
or potassium sorbate
Mold inhibitors in hard boiled and
peeled eggs. Usually 0.1% is added.
Sodium
silicoaluminate
Anticaking agent in dried egg
products at <2.0%. Serves as a
foaming or whipping aid in dried
white at 0.1%.
Source: Adapted from the American Egg Board.
c10.indd 184 1/14/2016 7:28:46 AM
CHAPTER 10 • Egg- and Milk-Based Foods • 185
Protein‐based gels, such as egg custards, are greatly
affected by the pH of the system. Hydrogen ions influence the
net charge of a protein and therefore influence the protein’s
solubility. As the pH of the system approaches a protein’s iso-
electric point (pI), the protein becomes less soluble. This pro-
motes aggregation and eventual coagulation. pI varies by
protein; therefore, the effect of pH varies depending on the
source of egg protein (whites versus yolks versus whole eggs).
An acidic pH of 5 produces the greatest gel strength and per-
centage of water release as protein molecules move closer
together, squeezing water out of the gel. In general, as pH
increases (becomes more alkaline), gel strength decreases
but water‐holding capacity of the system increases. Acid
added to egg white foam (meringue) in the form of cream of
tartar prevents over‐coagulation and keeps the foam elastic.
Acid added to whole egg lowers the coagulation temperature,
speeding coagulation. Custards made with acid‐containing
fruits or vegetables set faster.12
Egg proteins are also used in forming films to help adhere
batters and coatings to fried foods. Film‐forming is similar to
coagulation and gelling in that the interaction of protein
molecules produces stability. Disulfide bonding between egg‐
white proteins is important to this process. Unlike forming
gels by acidifying to the isoelectric point, film formation is
enhanced by raising the pH and heating to denature the pro-
tein and increase disulfide bonding. Water, sugars, and
starches can be added to increase the flexibility and elasticity
of the film so it is workable and not too brittle. Using whole
eggs instead of whites also decreases the strength of the film
and improves flexibility. Dried egg products and fresh liquid
eggs typically do not differ in their binding properties.
Many types of egg‐based coatings are applied to baked
goods, particularly breads and rolls. An egg‐based glaze typi-
cally prevents the crust from drying and becoming tough
while also enhancing the appearance. A glaze can be pre-
pared using egg white only or combinations of egg white,
yolk, or whole egg with salt, milk, water, or cream. Salt and
milk give a shiny surface to the product, while water and
cream yield a golden or brown appearance and softer texture.
Glazes are traditionally applied just before the product is
heated, but sometimes not until 15 minutes before the end of
cooking to prevent the crust from becoming too dark.
Emulsification
Surface active agents (surfactants) that enable immiscible
liquids like oil and water to form an emulsion are called
emulsifiers. The phospholipids, lipoproteins, and proteins
found in egg yolks make yolks excellent emulsifying ingredi-
ents. Low‐density lipoproteins (LDL), not lecithin (as is mis-
takenly believed), are the primary components that make egg
yolk such a strong emulsifying agent. In fact, the emulsifying
activity of whole egg and egg white are only one‐half and one‐
quarter, respectively, that of egg yolk alone.13 Factors that
influence the emulsification capability of egg yolk are freez-
ing, pasteurization temperature, drying temperatures, and
the presence of salts and acids.
Freezing and thawing of yolks results in denaturation and
irreversible gelation of yolk proteins, causing decreased
emulsifying capacity and stability. The addition of 10 percent
salt or sugar to yolks before freezing protects against gelation
and improves emulsifying capacity. Mayonnaise manufactur-
ers commonly use salted yolk as an emulsifier. Drying yolks
decreases emulsifying capacity because lipoproteins are
released from cell membranes during the drying process. The
addition of 5 to 10 percent sucrose before drying maintains
the emulsifying capacity of the yolk. In general, the addition
of salt or sugar increases emulsion stability by preventing gel-
ling before freezing or drying, while acids decrease emulsify-
ing capacity.14
Foaming
Foams are colloidal dispersions in which gas bubbles are dis-
persed in a liquid or solid phase. Food foams are created
through the action of surfactants that stabilize the air cells.
Eggs have an abundance of surfactants, so they are especially
effective in foam creation and stability. The production of
cakes, confections, and foamy egg dishes relies on this.
Examples include meringues (highly aerated egg whites con-
taining sugar and coagulated by heat), angel food cakes (also
called sponge or foam cakes), and soufflés. Egg whites and
yolks are both capable of producing foams; however, whites
are typically preferred because they contain insignificant lev-
els of lipids and therefore produce more stable foams. They
are also colorless and impart little flavor compared to egg
yolks or whole eggs.15 One example of an egg yolk foam is
zabaglione (Italian), or sabayon (French), a thick dessert
sauce made of egg yolks, sugar, and wine heated slowly under
constant beating. Zabaglione is eaten as is, used as a dessert
topping, and also used as an ingredient in the filling for
tiramisu.
Egg foams can be affected by many things, including the
method and degree of beating/blending/homogenization,
temperature, pH, fat, oil, salt, water, sugar, heating, addition
of surfactants (sodium lauryl sulfate, sodium oleate, and
others), esters (sucrose stearate, sucrose palmitate, and
others), addition of emulsifiers (lecithin, mono‐ and diglycer-
ide of fatty acids esters, and others), or stabilizers (citric acid,
guar gum, xanthan, and others). For example, up to a point
the volume of meringue foam increases with time of beating.
Maximum stability occurs at the point where the volume is
greatest. Continued beating past this point causes the foam
to lose its elasticity and become stiff and brittle, and eventu-
ally results in a separation of the foam’s structure, followed by
collapse.16
Surface tension of food systems is lowered by increasing
temperatures. Therefore, foaming rate and volume increase
in egg whites when beaten at room temperature versus refrig-
eration temperatures.17 Acid ingredients (cream of tartar,
lemon juice) help stabilize the foam by making it less prone to
over‐coagulation. Increasing the acidity of egg whites loosens
the protein structure. This keeps the foam elastic and stable
enough to entrap the air cells, and allows them to expand
c10.indd 185 1/14/2016 7:28:46 AM
186 • CULINOLOGY®
when heated, resulting in better volume. Acids also tend to
enhance the whiteness of egg‐based foams.
On the other hand, even the smallest amount of fat inhib-
its foaming and reduces volume. Egg whites containing less
than 0.05 percent yolk exhibit reduced foaming properties
and volume. This may be counteracted by adding freeze‐
dried egg whites. Salt decreases foam’s stability by weakening
the protein structure by tying up free water, while adding
water increases volume but decreases stability when dilution
reaches 40 percent or more.
The addition of sugar prevents overbeating by delaying
foam formation. Naturally, this increases whip time as well.
Sugar separates protein molecules and slows the bonding
process. It also stabilizes foam during heating by delaying
water evaporation until the protein structure has had time to
form. If sugars are added prior to heating, the functional
properties of the whites are protected against the effects of
heat. Heat gives permanence to (sets) the foam by inducing
the coagulation of the egg protein’s structure surrounding the
air cells trapped in the foam.
Surface tension is the property of a liquid’s surface that
allows it to resist an external force. It is the reason why oil
and water do not stay permanently blended in a solution
without the addition of an emulsifier or surfactant, which
decreases surface tension (or interfacial tension) between the
two liquids. The action of surfactants is not restricted to liq-
uids; they can also be used to assist in foam formation and
stabilization between a mixture of a liquid and a gas such as
meringue (egg white and air) or yolk‐containing foams.
Surfactants such as sodium lauryl sulfate (0.1 percent) are
used as a whipping aid in dried egg white. Triethyl citrate reduces
whipping time and improves volume in cakes containing yolks.
Hydrocolloids can also help stabilize foods that depend
on egg foams. Carboxymethyl cellulose prevents the collapse
of frozen soufflés and meringues as well as reduces syneresis,
and guar gum can be used to improve meringues in foods
cooked by microwave heating.18 Hydrocolloids provide thick-
ening and gelling of aqueous solutions, stabilizing foams,
emulsions, and dispersions, and preventing crystallization of
saturated sugar solutions.
Controlling Crystallization
Whole eggs, whites, yolks, or egg yolk solids are used in con-
fectionery products and ice creams to control ice crystal for-
mation and promote smooth texture and desirable mouthfeel.
Egg yolks in ice cream control density, hardness, and texture
by encouraging the formation of small ice crystals. With their
LDL content, they are excellent emulsifiers that help disperse
fat throughout the ice cream mix and prevent it from clump-
ing. Egg yolk also improves whipping properties of ice cream
mix to result in a desirable dry and stiff ice cream.
In candies, which are supersaturated sugar solutions, egg
whites act as interfering agents to retard sugar crystallization
and to form many fine sugar crystals for a smooth texture. Egg
white products improve whipping quality in aerated confections
and help interfere with sucrose crystal formation, which can
cause grainy texture and poor mouthfeel.19 Lipids in yolks add
flavor, tenderizing, and lubricating qualities in all confections.
Milk
Composition of Milk and
Milk‐Based Products
Milk is defined by the U.S. Food and Drug Administration
(FDA) as “the lacteal secretion (fluid from the mammary
gland of mammals who have recently giving birth), practi-
cally free from colostrum (the milk produced within the first
days of lactation), obtained by the complete milking of one
or more healthy cows.”20 Fresh raw milk contains 85 to 88
percent water, 3 to 4 percent protein, 4.6 to 5.2 percent
carbohydrate, 3 to 5 percent fat, and 0.7 to 1.0 percent miner-
als21 and provides amino acids, carbohydrates (mainly
lactose), calcium, and phosphorus. Because of milk’s unique
composition, it can be converted to manufactured products
such as cheese, fermented milk products, butter, and many
others.
Milk contains two primary classes of proteins, casein
and whey. Both are at the top of the Protein Digestibility
Corrected Amino Acid Score (PDCAAS) scale, with perfect
scores of 1.0 (along with egg white and soy protein). Caseins
are about 80 percent of the total protein in milk. Casein pro-
teins in their native form are not water soluble and not easily
coagulated by cooking. In addition, they precipitate at pH
4.6 and are the primary proteins that coagulate into curds
during cheese manufacture. Casein is actually insoluble in
milk. Rather, it exists as a stable colloidal dispersion of parti-
cles called casein micelles (see Figure 10.7), with the hydro-
philic (water‐loving) parts residing at the surface closest to
the milk and the hydrophobic (water‐fearing) parts oriented
in the micelle’s center. Calcium and phosphorus in milk are
normally present in an insoluble form that helps stabilize
the micelles. Reducing the pH of milk by adding acids like
lemon juice or vinegar, or as a result of microbial fermenta-
tion, solubilizes the calcium in the micelles; this, along with
the direct effects of acid on the protein, causes the micelles
to precipitate out of suspension and the milk to coagulate
and form curds. This reaction is commonly utilized in
cheese‐making.
Whey proteins make up approximately 20 percent of the
total protein in milk.22 Unlike casein, whey proteins remain
soluble at pH 4.6 (in fact, they are always soluble, regardless of
pH) and are in the whey fraction that remains after curd for-
mation during cheese‐making. They are easily coagulated by
heating and are responsible for the film that forms (and often
burns) on the bottom of the pan when milk is heated. They
are also responsible for initiating the formation of the skin
that develops on the surface of milk during extended uncov-
ered heating above 158°F (70°C). As whey proteins denature,
they become sticky and start to bind with casein and other
milk proteins. At the same time, the water in the milk starts
to evaporate. The proteins eventually become concentrated
enough to create a thin film at the top.
c10.indd 186 1/14/2016 7:28:47 AM
CHAPTER 10 • Egg- and Milk-Based Foods • 187
The carbohydrate in milk is the reducing sugar lactose, a
disaccharide composed of D‐glucose and D‐galactose. It is
the only carbohydrate of any significance in milk; for that
matter, it is the only carbohydrate of nutritional significance
in any food of animal origin. It is only one‐sixth as sweet as
sucrose and imparts only slight sweetness to fresh milk.
Being a reducing sugar, it reacts with amino acids to cause
non‐enzymatic browning through the Maillard reaction,
leading to color and flavor changes. Caramelization of the
lactose also leads to brown color and caramelized flavors.
FIGURE 10.7 Product composition of dairy ingredients.
Product Moisture Protein Lipid Carbohydrate Ash
%
Whole milk 88.0 3.2 3.5 4.6 0.7
Ultrafiltered milk 70.0–75.0 10.0–12.0 11.0–14.0 <5.0 <2.5
Ultrafiltered nonfat milk 80.0–85.0 10.0–12.0 <0.5 <5.0 <2.5
Ultrafiltered (diafiltrated) nonfat milk 80.0–82.0 16.0–17.0 <0.5 <1.0 <1.5
Sweetened condensed whole milk 27.0 7.8 8.0 55.2 1.8
Evaporated whole milk 74.0 6.5 7.5 9.8 1.4
Half‐and‐half 80.2 3.1 11.5 4.5 0.7
Light cream 74.0 2.9 18.3 4.2 0.6
Light whipping cream 62.9 2.5 30.5 3.6 0.5
Heavy cream 57.3 2.2 36.8 3.2 0.5
Plastic cream 18.2 0.7 80.0 1.0 0.1
Cultured sour cream 71.0 3.2 21.0 4.3 0.7
Butter 16.0 0.9 >80 0.1 2.1
Anhydrous milkfat <0.1 0.0 >99.8 0.0 0.0
Butter oil <0.2 0.3 >99.5 0.0 0.0
Dry butter powder <4.0 11.0 72.0 15.0 2.5
Dry whole milk powder 3.2 26.3 26.7 38.4 6.1
Dry nonfat milk powder 3.2 36.2 0.8 52.0 7.9
Dry buttermilk powder 3.0–3.5 32.0–34.5 5.5–6.0 49.5–50.5 7.5–8.0
Dry yogurt powder 3.0–5.0 33.0–36.0 1.25–2.0 50.0–51.5 7.0–8.0
Dry cultured dairy solids 3.0–5.0 22.0–33.0 0.0–2.0 52.0–66.7 7.0–8.0
Dry sweet whey 3.2 12.9 1.1 74.4 8.4
Dry acid whey 3.5 11.7 0.5 70.0 10.8
35% Whey protein concentrate 4.6 36.2 2.1 46.5 7.8
80% Whey protein concentrate 4.0 81.0 7.2 3.5 3.1
Dry whey protein isolate 3.7 91.5 0.5 0.8 3.7
Dry milk protein concentrate (MPC42) 3.5 42.0 1.0 46.0 7.5
Dry milk protein concentrate (MPC75) 5.0 75.0 1.5 10.9 7.6
Dry milk protein concentrate (MPC85) 4.9 85.0 1.6 1.0 7.1
Crystallized lactose (food grade) 4.5–5.5 0.1–0.6 0.0 99.0 0.1–0.3
Source: Adapted from Commercial Industry Specifications and M. Walsh, D. McMahon, and S. E. Duncan, "Milk and Dairy Products," Ch. 19
in Food Chemistry: Principles and Applications, ed. Y. H. Hui (West Sacramento, CA: Science Technology Systems, 2007), 19.1–19.23.
c10.indd 187 1/14/2016 7:28:47 AM
188 • CULINOLOGY®
Fat exists in milk as an oil‐in‐water emulsion, with the fat
globules or droplets dispersed throughout the milk serum.
Triacylglycerols (triglycerides) make up 96 to 98 percent of
the lipids in milk, the rest being phospholipids, cholesterol,
monoacylglycerols, and diacylglycerols. The fatty acid com-
position of the triacylglycerol depends on the cow’s diet and
the reactions that occur during digestion. Thirteen fatty
acids are present in milkfat at levels greater than 1 percent.
Close to 75 percent of the fatty acid content in milk is com-
prised of saturated fats, with the remaining 25 to 30 percent
unsaturated. Milk contains significant levels of short‐chain
fatty acids—butyric (C4), caproic (C6), caprylic (C8), and
capric (C10).23 Those that have the smallest molecular
weight are both the most volatile and the most flavorful.
When they are cleaved from the glycerol backbone by the
action of native and microbial lipases (enzymes that remove
lipids from glycerol), they cause undesirable rancid flavors in
milk but desirable aged flavors in cheeses such as Parmesan
and Romano.
In its fresh state, milk triacylglycerol is protected from the
action of lipases by a milkfat globule membrane coat.
However, if fresh raw milk is subjected to excessive agitation
or foaming, this protective coat is disrupted, rendering the fat
susceptible to lipase activity. In milk, this is termed hydrolytic
rancidity, and the short‐chain fatty acids give the milk a fla-
vor described as baby vomit. This is typically not a problem in
pasteurized homogenized milk products because the native
lipase is easily inactivated by the heat of pasteurization.
However, as milk is stored, spoilage microorganisms can
grow and their enzymes can also produce this defect, so
proper temperature control is necessary.
Nutrition
Milk is highly nutritious yet grossly underutilized in the
American diet. It provides a compositionally balanced
makeup of the macronutrients carbohydrate, protein, and fat,
and contains 10 to 30 percent of daily values (DV) for nutri-
ents including calcium, phosphorus, vitamin D, potassium,
magnesium, vitamins A, B
12
, riboflavin, and niacin. For every
8 ounces of milk there are 12 g carbohydrate and 9 g protein
(contributing 16 percent of the DV of protein). The fat content
depends on whether the milk is whole (4 percent), 2 percent,
1 percent, or fat free, and calories vary accordingly (from 83
calories per cup for skim milk to 146 calories per cup for
whole milk).
Carbohydrate in milk is almost entirely in the form of
lactose, which requires the enzyme lactase for digestion. If
lactase is not available, the disaccharide moves through the
large intestine intact, where gut microbiota access and
metabolize it. This can result in excessive gas production and
possible diarrhea, symptoms of lactose intolerance. Lactose
intolerance is not a rare occurrence; it is estimated that
65 percent of the global population is affected (especially
Asians and Africans). Lactose‐free milk products are prepared
by adding lactase enzyme to the milk during processing to
break the disaccharide into individual glucose and galactose
molecules, allowing absorption to occur and relieving symp-
toms of lactose intolerance. Because lactose is water soluble,
low‐fat and skim milks have higher levels of lactose than full-
fat milks or creams, in which there are higher levels of milkfat.
Even butter contains trace amounts of lactose unless it is cul-
tured or clarified. Lactose levels in hard cheeses made from the
curds after the whey has been removed are extremely low.
Levels in soft‐ripened, or well‐aged cheeses are also quite low
since the lactose has been broken down by microbial fermen-
tation. In addition, fermented milk‐based products such as
yogurt are well tolerated because yogurt contains lactase
generated by the bacterial cultures used to produce it. It
should be noted that dried milk solids and whey powders
commonly used for protein enhancement or as bulking
agents in many processed foods contain large quantities of
lactose as the water‐soluble components in fluid milk
become concentrated during the drying process.
Other star nutrients in milk are calcium, phosphorus, and
vitamin D. These micronutrients function in bone growth
and maintenance. Bone is in a constant state of turnover,
depositing and withdrawing calcium based on bone cell age,
blood calcium, phosphorus, and parathyroid hormone con-
centrations. Vitamin D is naturally present in small amounts
in milk but beginning in 1933 was augmented to combat
rickets (bone disease due to lack of vitamin D; it causes poor
bone development, particularly in the pelvis and legs, leading
to deformity, fractures, and difficulty walking). This was the
second food fortification to occur in the United States, after
iodine in 1924 was added to salt to prevent goiter (enlarge-
ment of the thyroid gland). Vitamin D enhances the absorp-
tion of calcium from the gastrointestinal tract. When blood
calcium levels fall, parathyroid hormone is produced, signal-
ing the release of vitamin D, which interacts with gut epithe-
lial cells to increase calcium absorption. Without adequate
vitamin D, this cycle continues, but the calcium is released
from the bones. Eventually, this leads to excessive or incom-
plete bone turnover, resulting in osteodystrophy (abnormal
development and loss of bone). Milk is the largest contributor
of calcium in the diet.24 One 8‐ounce glass provides 30 percent
of the DV of calcium and 25 percent of the DV for vitamin D
and phosphorus.
Calcium can, however, interfere with the ability to absorb
iron, thereby increasing the risk for iron‐deficiency anemia.
For this reason, addition of milk to high‐iron foods may limit
the bioavailability of the iron. This should not prohibit the
use of milk for building a mixed dish or food item, however, as
there are plenty of opportunities to obtain iron from other
sources in the diet.
Additional nutrients in milk include blood builders like
potassium and magnesium, which work to maintain fluid
and electrolyte balance in the blood. Adequate amounts of
these micronutrients are shown to aid in the management
of blood pressure.25 Finally, vitamin B
12
in milk is involved in
the production of red blood cells, further supporting blood
health.
c10.indd 188 1/14/2016 7:28:47 AM
CHAPTER 10 • Egg- and Milk-Based Foods • 189
The Value of Dairy
Products in Food
Product Development
An enormous range of dairy ingredients is available to the
food product developer. These include:
Butter ingredients, including whipped, cultured, light, salted,
and unsalted; and anhydrous milkfat, butter oil, lipolyzed
butter, butter powder, and buttermilk powder.
Cream ingredients, including half‐and‐half (10.5 to 18 percent
fat), light cream (18 to 30 percent fat), light whipping cream
(30 to 36 percent fat), heavy cream (>36 percent fat), plastic
cream (cream separated a second time to yield 80 percent fat,
which is crystallized and plastic), cultured sour cream, acidi-
fied sour cream, and dry cream (40 to 75 percent fat).
Milk protein concentrate ingredients, including whole milk
powder, milk protein concentrate, nonfat dry milk, and ultra‐
filtrated milk. Milk protein concentrate ingredients are avail-
able in protein levels from 42 to 85 percent and are made from
milk, so they contain the normal ratio of milk casein and whey
proteins. Nonfat dry milk ingredients come in three classifica-
tions: high heat, medium heat, and low heat. The variation in
heat treatment occurs during the concentration step, before
spray‐drying. This affects the color, flavor, and solubility of the
ingredients. High‐heat nonfat dry milk is typically used in bak-
ing applications because of its color and reduced solubility
and because it does not detrimentally affect loaf volume (milk
can have a reducing effect on gluten if not strongly heated).
When milk powder is reconstituted for beverages or fer-
mented dairy products, low‐heat nonfat dry milk is used
because of its mild flavor and ease of reconstitution. Ultra‐fil-
trated milk ingredients come in whole and skim milk concen-
trates as well as reduced‐lactose products. Ultra‐filtration is a
membrane process where compounds of different sizes are
selectively removed. At its most basic, water and minerals are
removed to concentrate the milk without heating (which is
required in making condensed milk). With increased mem-
brane pore sizes, lactose can also be removed.
Whey ingredients, including sweet whey, acid whey, 35 percent
whey protein concentrate, 80 percent whey protein concen-
trate, and whey protein isolate with 90 percent protein. The
designator “sweet” refers to whey produced from rennet‐
coagulated cheeses, such as cheddar and Swiss. Acid whey is
the dried product of acid‐coagulated cheeses, like cottage
cheese. Both whey products contain lactose; their main differ-
ence is in mineral composition. Acid whey has a higher level of
calcium. Lactose and minerals are further removed when
whey is concentrated to produce isolates.
Yogurt powdered products, including yogurt powder and cul-
tured dairy solids.
Cheese ingredients, including natural, pasteurized process
cheese, cheese powders, enzyme‐modified cheeses, and
cheese analogs.
Casein ingredients, including sodium and calcium caseinate,
water‐soluble versions of casein used as a high‐quality
protein supplement in a wide variety of processed foods and
baked goods, as a bulking agent in instant soup mixes
and coffee creamers, and as an emulsifier in processed meats
and dairy beverages.
Milkfat Function
Milkfat can be sourced in liquid (full‐fat milks and creams),
solid (butter), and dried forms (butter and whole milk
powders). The function of milkfat is a result of its fatty
acid composition and other constituents (phospholipids).
Applications include baked products, confections, sauces
and soups, and frozen products. The unique dairy flavor
notes of milkfat enhance the richness of foods when com-
pared to other fats. Milkfat ingredients are also associated
with the production of caramel, praline, and toffee flavors. It
is highly compatible with cocoa butter and therefore can be
used to reduce cost in the formulation of confections. Heat
can produce varied flavor notes from milkfat in the produc-
tion of different sauces; lightly melted whole butter is typi-
cally used in cream and white sauces, clarified butter is used
as the basis for emulsified sauces such as hollandaise and
béarnaise to provide rich dairy notes; slightly overheated but-
ter provides roasted, cooked notes that complement brown
sauces and gravies; and browned butter contributes flavor
notes that complement barbecue, traditional Cajun, and
smoke‐flavored sauces.
Milkfat works well as a flavor carrier for spices, sweet and
savory flavors, herbs, and other fat‐soluble ingredients. The
narrow melting range of butter ensures the quick release of
oil‐soluble flavors in a meltaway effect that imparts smooth
mouthfeel properties.26 Milkfat enhances the structure of
cakes and pastries, helps retard fat bloom in chocolates, and
enhances aeration in icings and cakes. It has excellent layer-
ing properties that disrupt the formation of three‐dimen-
sional gluten networks in laminated dough products,
resulting in desirable flaky (rather than chewy) crusts.27
In frozen products, milkfats provide functions that cannot
be duplicated by vegetable oils, including their ability to form
foams when whipped and their unique texture when frozen.
This is due to the variability of the fatty acid composition of
the triacylglycerides, which confers a range in the crystalliza-
tion properties of the fat.28 Highly unsaturated fats of vegeta-
ble oils have a lower melting temperature than saturated fats.
This means they do not crystallize unless the temperature
is very low. Dairy fats have a mix of fatty acids, including
saturated fat, which allows for some fat crystallization at
refrigeration temperature after pasteurization. When the
tem perature is lowered further, the lower melting fatty acids
will further crystallize. This property is important when mak-
ing whipped cream and is the reason the cream must be very
cold during whipping. The crystallized fat helps stabilize the
air cells in the whipped product. When freezing dairy bases
to make ice cream, the pasteurized mix is refrigerated for at
c10.indd 189 1/14/2016 7:28:47 AM
190 • CULINOLOGY®
least 4 hours to allow some of the fat to crystallize prior to
freezing and whipping in air. After emerging from the ice
cream freezer, about 40 percent of the water is frozen and the
ice cream is like soft‐serve.29 Water and fat further crystallize
in the freezer during the process known as hardening. The
texture of ice cream can be contrasted to that of gelato, which
contains some nondairy fat and thus is stickier and softer.
A point to note for the Culinology® professional making ice
cream using purchased whipping cream: Whipping cream is
typically not homogenized because this reduces its ability to
make a high‐quality whipped cream. When making ice cream,
however, it is important to homogenize the ice cream base
prior to freezing to prevent a churned effect in which the ice
cream has a greasy, buttery texture. This might not be possible,
however, so care should be taken to not over‐mix the product.
Water‐Holding Capacity
Water‐solute interactions have a significant impact on the
properties of foods. Methods or ingredients that bind or
entrap water in foods can be used to lower cost by increasing
yield and reducing syneresis. This also improves product
quality in that moisture and mouthfeel are enhanced by
increasing the viscosity or perceived moistness of a product.
Water‐holding capacity is the term for the ability of a matrix
of molecules to physically entrap large amounts of water in a
manner that inhibits exudation under the application of an
external force.30 Dry milk ingredients bind a certain amount
of water when rehydrated, and when heated, the proteins
denature and unfold, increasing the viscosity and water hold-
ing capacity. This is a factor in the production of viscous food
products such as beverages (chocolate milk and fermented
milk drinks), thick soups and custards, baked goods (bread
and cakes), confections (puddings, custards, and flan), dairy
products (yogurt), meat products (sausages and other formu-
lated meat products), salad dressings, and sauces.
Adding dry milk ingredients to bakery formulations
results in softer doughs because the ingredients bind water
and interfere with gluten interactions. The results are cookie
or cake doughs that are easier to machine and extrude and
baked products with a softer texture. Dairy proteins provide
structural support in salad dressings, especially creamy‐style
products. Their ability to bind water is also important in
formulating reduced‐fat dressings because they contribute
fat‐like attributes, such as lubricity and creamy mouthfeel.31
High‐heat dairy powders do not depress bread loaf vol-
ume because they are less soluble than low‐heat powders
and compete less with gluten for the available water. They
also reduce water separation and improve the textures of
meat products like bologna and sausage. Medium‐heat dry
dairy ingredients produce the firm, chewy texture of many
confections and increase yield through improved water hold-
ing. Low‐ and medium‐heat dry dairy ingredients help stabi-
lize the viscosity of pasteurized process cheese and increase
the firmness of the coagulum in fermented milk products like
yogurt. They also provide structural support to extruded fro-
zen products like ice cream.
Color and Browning
Dairy protein and carbohydrate ingredients can contribute
to the color of foods and participate in Maillard browning.
During baking or cooking, the protein’s amine groups react
with lactose and other reducing sugars to create brown color
in baked goods and sauces. Milkfat contributes creamy color
that balances the color of thickened products such as sauces,
soups, salad dressings, and beverages. The color contributed
can be due to carotenoid pigments in the milkfat that come
from the cow’s diet and/or to the light‐scattering effects of fat
in the milk. When fat is removed from milk, the milk appears
slightly green or blue because it doesn’t scatter the light as
well. This is commonly seen in the bluish appearance of skim
milk. (The casein micelle tends to scatter blue wavelengths,
and the riboflavin in milk has a greenish tint.) Dairy ingredi-
ents also contribute opacity, which helps give products the
appearance of a richer texture, especially in reduced‐ fat
items.32
Emulsification
Dairy proteins have both hydrophilic and hydrophobic
groups; therefore, they are used successfully at oil‐water
interfaces to form and stabilize emulsions. Emulsification
properties are enhanced by controlled protein denaturation.
Heating promotes the unfolding of the dairy proteins to
expose hydrophobic amino acid residues that encourage
them to orient at the oil‐water interface. Emulsification func-
tionality improves when these interfaces are further pro-
tected by adsorption of added surfactants (such as sorbitan
esters, monoglycerides, lecithins, and hydrocolloids) around
the oil droplets, and partially lost again by re‐coalescence of
those oil droplets that are not protected quickly enough. Re‐
coalescence of the fat droplets leading to emulsion break-
down is an important phenomenon in emulsions stabilized
by proteins because of the relatively slow development of the
protective surfactant film around new emulsion droplets.
Dairy ingredients are used to stabilize emulsions in appli-
cations for baked goods, beverages, dairy products, meats,
salad dressings, soups, and sauces.33 Their ability to stabilize
oil‐water emulsions is affected by pH, temperature, the
extent of protein denaturation, the ionic strength of the aque-
ous phase (especially the presence of calcium), and the pres-
ence of other emulsifiers. The caseins in milk are disorganized
and flexible molecules, whereas the whey proteins are globu-
lar and compact, and both act as emulsifiers. Lecithin in
milkfat also contributes to emulsification because it improves
fat dispersion. The high phospholipids and 4 percent fat con-
tent of buttermilk powder (the product of butter‐making, not
the fermented milk product) make it an excellent choice for
emulsifying and adding milk solids to food products.
Gelation and Thickening
Milk proteins have several mechanisms that can be used
to form gels and increase viscosity. Caseins form enzyme‐
induced gels by the action of chymosin (also known as
c10.indd 190 1/14/2016 7:28:47 AM
CHAPTER 10 • Egg- and Milk-Based Foods • 191
rennin), an enzyme in rennet that acts on kappa casein. The
enzyme cleaves the carbohydrate portion of the molecule
and causes the casein micelle to become unstable and to
coagulate. This coagulation method is used in the produc-
tion of rennet‐coagulated cheeses such as cheddar, Swiss,
and mozzarella. Caseins also gel if the pH is reduced to its
isoelectric point (pH 4.6). If this acidification is done slowly
and without agitation, the resulting gel can produce a prod-
uct such as yogurt, or it can be cut, drained, and pressed to
produce acid‐coagulated cheeses, like cottage cheese and
cream cheese.
Whey proteins form gels when heated to high tempera-
tures (approximately 180°F [82°C]) with the addition of acid,
or at room temperature if acid is gradually added after the
protein is treated by high temperatures. Gelation is a two‐
step mechanism that involves an initiation step involving the
unfolding or dissociation of protein molecules by the mecha-
nisms just described, followed by an aggregation step in
which association or aggregation occurs between protein
molecules, resulting in gel formation. For the formation of a
highly ordered gel, such as in yogurt, it is essential that the
aggregation step proceed slowly or else a precipitate, rather
than an ordered gel, will form. Ricotta cheese is an example of
aggregation occurring under stirring, so instead of a gel, a
granular precipitate is formed that does not hold water,
which is removed as whey.
Dry dairy ingredients are used in confection applications
to form rigid, heat‐induced irreversible gels that hold water
and fat and provide structural support to confections such as
nougat, frosting, and creams. The type of aggregation affects
the gel’s opacity, an important property in many candies and
confections. Medium‐heat dairy ingredients provide better
flavor than high‐heat dairy products, which have a strong
cooked taste. Dry dairy ingredients also help improve viscos-
ity in processed cheese and create stable gels in fermented
milk products. Low‐heat products provide better gelation for
products such as yogurt without impacting flavor profiles.
The texture, firmness, and chewiness of many processed
meat products are enhanced by heat‐induced gels from high‐
heat dairy proteins because meat flavors mask the more
intense cooked flavors of high‐heat dairy ingredients.
In beverage applications, dry milk ingredients add to the
nutritional profile and help thicken shake‐type drinks and
nutraceutical beverages. Low‐heat dairy powders help thicken
while providing a mild flavor profile that blends well with
other flavors. For UHT (Ultra High Temperature)‐ processed
beverages, product formulators can utilize low‐heat products
to prevent gelation during extended room‐temperature
storage.34
Whipping and Foaming
An important attribute of milk proteins is their surface‐active
behavior. Caseinates and whey protein concentrates are excel-
lent at enhancing the whipping and stabilizing of foams. They
easily adsorb to fat globule–water interfaces during homogen-
ization of milk and to the air bubble–cream interface during
whipping. A rapid diffusion of protein to the air‐water interface
reduces surface tension, followed by partial unfolding of the
protein; this is essential to forming protein‐based foams, like
the caseinate foams used as dessert toppings. As dairy protein
concentration increases, foams become denser, with finer,
more uniform air bubbles.
Whipping properties are affected by protein concentra-
tion, protein state (denatured or not), pH, presence of cal-
cium or other salts, heat treatment (if any), and the presence
of lipids. In the creation of a protein‐based foam, lipids reduce
the volume and stability (such as when whipping egg whites,
where any lipid from the yolk is detrimental). In baking appli-
cations, such as cakes, the addition of either low‐ or high‐heat
nonfat dry milk creates a desirable light, even texture by
forming dense, stable foams with finer, more uniform bubble
distribution.35 Foaming beverages, such as eggnog and shake‐
type drinks, benefit from low‐heat dairy powders to increase
the solids and viscosity so air cells are retained when the
product is shaken or whipped. Milk proteins are also a major
help in stabilizing the foams of frozen desserts like ice cream,
whipped toppings, and mousses.36
Cheese Applications
Cheese ingredients are commonly used to add flavor to for-
mulated food products. Cheese is generally defined as “the
fresh or matured product obtained by draining the whey after
coagulation of casein.” Casein is coagulated through the
application of acid (produced by the fermentation of lactose
by microbial cultures or directly added) or the proteolytic
enzyme chymosin, found in rennet. Cheese can be produced
from whole, 2 percent, 1 percent, and nonfat milk. Different
ingredients and processes during the manufacture and aging
result in a wide variety of cheeses with distinctive texture, fla-
vor, and functional properties. More than 200 varieties of
cheese are produced in the United States, and more than
1400 varieties are catalogued in the World Cheese Exchange
Database. Cheese is categorized as natural or processed, rip-
ened or fresh, and soft to hard.
Natural cheese is a general classification for cheese made
directly from milk. In fresh, unripened (unaged) cheese, the
curd is separated from the whey and formed into cheese
immediately for use or consumption within a few weeks.
Ripened cheeses are aged to allow flavor development from
the enzymatic breakdown of fats and proteins. Ripened
cheeses may also use adjuncts such as enzymes, secondary
bacterial flora, yeast, and molds to create unique flavors for
specific cheese varieties. Natural cheeses are categorized
according to their moisture content and degree of hardness.
Soft cheeses include Brie, Camembert, ricotta, and cottage
cheese. Semisoft cheeses include blue, brick, feta, Havarti,
Monterey Jack, mozzarella, Muenster, and provolone. Hard
cheeses include cheddar, Colby, Edam, Gouda, and Swiss.
Very hard cheese varieties include Parmesan and Romano.
When choosing natural cheese as an ingredient, it is impor-
tant to understand how it will perform in a finished product
based on its age and storage conditions.
c10.indd 191 1/14/2016 7:28:47 AM
192 • CULINOLOGY®
Processed cheeses are made by blending one or more nat-
ural cheeses into a homogenous mass, heating the mix, and
adding water, flavors, and emulsifying salts—typically sodium
citrate and a variety of sodium phosphates. These reduce the
calcium interactions with the milk proteins and improve
emulsification of the fat by the protein. Processed cheeses
typically maintain smooth, creamy, homogenous melting
characteristics during heat processing, unlike most natural
cheeses. Pasteurized processed cheeses include American
cheese, cheese spreads, and cheese foods. Cold‐pack cheese
is a blend of natural cheeses processed without heat, but
often with added flavorings and seasonings.
Cheese powders, or dehydrated cheeses, are prepared
using a single cheese variety or a blend of cheeses to produce
standardized flavor characteristics. Products may be all
cheese or a blend of cheese with other dairy ingredients such
as whey or milk powder, starches, or other carbohydrates, fla-
vors, or colors. Typical applications for cheese powders
include prepared dry mixes, sauces, and topical seasonings
for snack foods.37
Enzyme‐modified cheeses are industrial flavor ingredi-
ents that blend food‐grade lipases or proteases with natural
cheese to intensify cheese flavor development. These
enzymes accelerate the breakdown of fats to produce
increased levels of more flavorful free fatty acids and break
down proteins to produce increased levels of more flavorful
peptides and amino acids. They are much stronger in taste
then the natural cheeses they are made from and more eco-
nomical to use than natural cheese due to their intense fla-
vor. They are available in paste or powder form. Applications
include flavor enhancement of pasteurized process cheese
and cheese sauces, salad dressings, and snack foods.
Applications
Culinology® Case Study
in Product Development
Using Egg Products
Whether you describe it as Culinology® or molecular gas-
tronomy, Chef Wylie Dufresne’s creation of Eggs Benedict at
the chef/owner’s wd~50 restaurant is a wonderful study of
how combining science knowledge and culinary skill can
advance food innovations. Dufresne’s Eggs Benedict recipe
uses the coagulation chemistry of proteins and hydrocolloids
to create a dish of deep‐fried hollandaise sauce, egg yolk
“morsels,” and a crisp wafer of Canadian bacon.38
In an ABC Nightline interview, Dufresne described how his
search for understanding the science of food and its prepara-
tion fueled his fascination with eggs and their use as a food
and ingredient:
Every now and then I find a new approach to working with
eggs, and it’s just really exciting.. . . [T]hey’re fascinating from
a technical standpoint. The way an egg behaves and what
you can do to an egg and the various textures you can get.
The fact that there are two parts to it, basically the yolk and
the white. It gives you an incredible repertoire of things to
work with.39
Eggs Benedict Recipe
Chef/owner Wylie Dufresne, wd~50, New York
ABC Nightline Platelist and adapted by Starchefs.com
(Dufresne, 2008)
Yield: 4 servings
Egg Yolks
Raw egg yolks 10
Salt to taste
Cayenne, ground to taste
Preparation
Season the unbeaten yolks with salt and cayenne to taste.
Fill a 1 × 12‐inch plastic sleeve with the yolks and tie the top
with string to secure. Let stand upright for 2 hours to allow
any air to rise to the top. Cook the yolk‐filled sleeves upright
in a water bath for 17 minutes at 158°F (70°C). Then ice
down the sleeves and portion the cooked yolk “tubes” into
1‐inch‐long cylindrical portions.
FRIED HOLLANDAISE
Seasoned Base
Raw egg yolks 10 g
Salt to taste
Lemon juice to taste
Preparation
Mix the egg yolks, salt, and lemon juice well in a blender. Set
aside in the blender.
Part I
Sodium hexameta phosphate 2 g
Citric acid 0.6 g
Gellan gum or low‐acyl gellan gum 3.5 g
Water 170 g
Preparation
Blend sodium hexameta phosphate, citric acid, gellan gum,
and water together and bring to a boil in a small pot. Pour
into a container and cool until completely set.
Part II
Water 110 g
Ultrasperse M (modified starch) 10 g
Preparation
Mix water and Ultrasperse M together well and reserve.
Part III
Gelatin, bloomed 60 g
Butter, melted 640 g
Preparation
Dissolve the gelatin in the melted butter.
c10.indd 192 1/14/2016 7:28:47 AM
CHAPTER 10 • Egg- and Milk-Based Foods • 193
melting cheeses such as mozzarella, cheddar, Colby, and
Monterey Jack); and low pH (around 4.6—these acidic cheeses
have a pH around casein’s isoelectric point, which causes
them to soften but not flow, such as feta, cream cheese, and
cottage cheese). The goal was to develop methods to manipu-
late the melt characteristics of the mid‐ and low‐pH natural
cheeses to make them more suitable for applications that
require high‐temperature cooking (for example, deep frying
and microwaving).
The researchers were able to modify existing cheese‐man-
ufacturing processes to strengthen protein bonds, which
reduced their melt characteristics. Modifications included
higher pasteurization temperatures and homogenization of
the milk prior to the cheese‐manufacturing process. The
higher heat treatments increased protein denaturation and
cross‐linked structures, which inhibited the flow properties
of the natural cheese. Homogenization caused fat globules to
be coated with proteins, which resulted in a more rigid struc-
ture that decreased melt and flow. Cheese manufacturers can
now use pH control, high‐temperature pasteurization, and
homogenization to control melt in natural cheeses, turning a
natural high‐melting cheese with a mid‐range pH, like
mozzarella, into a non‐melting product for applications like
deep‐fried cheese sticks.40
Development of Gourmet Butter
for Foodservice Applications
More than 120 compounds give butter its unique flavor ;
however, the five biggest contributors are short chain fatty
acids, lactones, methyl ketones, diacetyl, and dimethyl
sulfide. Of these, lactones and methyl ketones are the
main components responsible for the flavor of butter in
heated applications. Although initially present at levels
below their flavor threshold value (FTV), on heating, the
total concentration of both lactones and methyl ketones
exceeds their FTV. The two compounds also react in a syn-
ergistic manner, providing a full, rich butter flavor. Methyl
ketones and lactones also interact with the flavors devel-
oped through the Maillard reaction during roasting and
baking. But it is the combination of all the flavor com-
pounds in butter that creates its overall appeal. Butter is
also an excellent flavor carrier for spices and other fat‐sol-
uble ingredients—a good way to bring added flavor to fin-
ished products.
Gourmet butter, also known as compound butter or beurre
composé, is a flavored butter with a variety of uses. Compound
butters have been made for centuries; two of the better
known are maître d’ hôtel (parsley and lemon) and chivry
(herbs). Butter is softened at room temperature and mixed
with supplementary ingredients. It can be made inexpen-
sively by adding just a few chopped herbs, or more expen-
sively by using ingredients such as black or white truffles.
After the butter is flavored, it can be used as is or reformed by
rolling in parchment paper or in a plastic tube and refriger-
ated until firm enough to slice.
Fried Hollandaise Preparation
Slowly add half of Part III (gelatin–melted butter mixture) to
the seasoned egg yolks in the blender as you would a classic
hollandaise. Then, using a bowl and whisk, blend Part II
(water and Ultrasperse M mixture) into the remaining half of
Part III (gelatin–melted butter mixture). Add the ingredients
from the bowl to the ingredients in the blender. The blender
should be running throughout all of the steps. At this point a
thick and creamy sauce should have formed. Adjust the
seasoning with salt and lemon juice. Pour the sauce into a
shallow baking tray lined with plastic and allow to chill
overnight. Cut the hollandaise into cubes and bread them
using a classic three‐step process: flour, egg wash, and
English muffin crumbs. Reserve the breaded cubes in the
freezer.
CANADIAN BACON
Canadian bacon 100 g
Oil for frying As needed
Preparation
Thinly slice the bacon on a deli slicer and fry in oil at 375°F
(191°C). Pat dry and reserve.
Dish Assembly
Gently warm egg yolk portions (prepared in step 1) in oven
(low temperature). Pan‐fry cubes of hollandaise and then
place in oven to warm through. Place 3 pieces each of egg
yolk morsels and fried hollandaise cubes on plate and
garnish with bacon, chives, and salt.
Culinology® Case Studies
in Product Development
Using Milk Products
Creating Natural Cheeses with Varied
Melting Characteristics
Many product developers turn to processed cheese products
when they need a cheese ingredient with controlled melting
characteristics. The melting characteristics can be engineered
by manufacturers through the use of emulsifying salts and
other ingredients that aid or hinder melting characteristics in
formulated foods. However, increasing demand from consum-
ers for manufactured food products with more natural ingredi-
ents has led product developers to search for sources of natural
cheese products with controlled melting characteristics. In
response to this demand, researchers at the Wisconsin Center
for Dairy Research are developing manufacturing methods for
controlling the melt characteristics of natural cheeses.
The pH balance of natural cheeses affects protein binding,
which directly affects melt characteristics. Natural cheeses
fall into three basic pH categories: high pH (around 6.4—
these are low‐melting cheeses such as Hispanic queso fresco
and queso blanco); mid pH (around 5.3—these are excellent
c10.indd 193 1/14/2016 7:28:47 AM
194 • CULINOLOGY®
These butters can be melted on top of meats and vegeta-
bles, used as a spread, or used to finish various sauces. Using
compound butters to finish a sauce or soup not only adds
flavor, but also phospholipids and lipoproteins that act as
natural emulsifying agents, contributing to a smooth, creamy
mouthfeel. In addition, gourmet butters can be used for sau-
téing, in baked goods, and for stuffing or basting. Many
upscale restaurants place a variety of flavored butters on
their tables for spreading on bread.
In addition to restaurant applications, gourmet butters
also provide food manufacturers with a wide variety of
options for new product development. New dishes may be
invented or a facelift given to old favorites.
Conclusion
Unmatched for nutritional value, affordability, and availability,
and with a wealth of functional capabilities in both food and
beverage applications, egg‐ and dairy‐based ingredients are
essential components in the Culinology® professional’s tool-
box. Learning to use them effectively takes experimentation
and practice, but the benefits far outweigh the investment
in time.
1E. C. Y. Li‐Chan, W. D. Powrie, and S. Nakai, “The Chemistry of Eggs and Egg
Products,” in Egg Science and Technology, W. J. Stadelman and W. J.
Cotterill, eds. (Binghamton, NY: Food Products Press, 1995), 105–175;
Y. Mine and M. Yang, “Eggs,” in Food Chemistry: Principles and Applications,
Y. H. Hui, ed. (West Sacramento, CA: Science Technology Systems, 2007),
26.1–26.20.
2American Egg Board, Egg Science and Technology Lesson Plan, vol. 2 (Park
Ridge, IL: American Egg Board, 2008).
3Li‐Chan, Powrie, and Nakai, “The Chemistry of Eggs and Egg Products,”
105–175. Mine and Yang, “Eggs,” 26.1–26.20.
4Y. Mine and M. Yang, “Eggs,” in Food Chemistry: Principles and Applications,
Y. H. Hui, ed. (West Sacramento, CA: Science Technology Systems, 2007),
26.1–26.20.
5F. B. Hu et al., “A Prospective Study of Egg Consumption and Risk of
Cardiovascular Disease in Men and Women,” Journal of the American
Medical Association 281 (1999): 1387–1394; D. J. McNamara, “The Impact of
Egg Limitations on Coronary Heart Disease Risk: Do the Numbers Add
Up?” Journal of the American College of Nutrition 19 (2000): 540S–548S; R. M.
Krauss et al., “A Review of Scientific Research and Recommendations
Regarding Eggs,” Journal of the American College of Nutrition 23 (2004):
596S–600S; L. M. Barraj et al., “A Comparison of Egg Consumption with
Other Modifiable Coronary Heart Disease Lifestyle Risk Factors: A Relative
Risk Apportionment Study,” Risk Analysis 29, no. 3 (2008): 401–415;
A. Mente, L. de Koning, H. S. Shannon, and S. S. Anand, “A Systematic
Review of the Evidence Supporting a Causal Link Between Dietary Factors
and Coronary Heart Disease,” Archives of Internal Medicine 169, no. 7
(2009): 659–669.
6Krauss et al., “A Review of Scientific Research and Recommendations
Regarding Eggs,” 596S–600S; A. H. Lichtenstein et al., “Diet and Lifestyle
Recommendations Revision 2006: A Scientific Statement from the American
Heart Association Nutrition Committee,” Circulation 114 (2006): 82–96.
7S. Y. Oh et al., “Eggs Enriched in Omega‐3 Fatty Acids and Alterations in
Lipid Concentrations in Plasma and Lipoproteins and in Blood Pressure,
American Journal of Clinical Nutrition 54 (1991): 689–695; Z. Jiang and
J. S. Sim, “Consumption of n‐3 Polyunsaturated Fatty Acid–Enriched
Eggs and Changes in Plasma Lipids of Human Subjects,” Nutrition 9, no.
6 (1993): 561–562; L. K. Ferrier et al., “Alpha‐Linolenic Acid– and
Docosahexaenoic Acid–Enriched Eggs from Hens Fed Flaxseed:
Influence on Blood Lipids and Platelet Phospholipid Fatty Acids in
Humans,” American Journal of Clinical Nutrition 62 (1995): 81–86; D. J.
Farrell, “Enrichment of Hen Eggs with n‐3 Long‐Chain Fatty Acids and
Evaluation of Enriched Eggs in Humans,” American Journal of Clinical
Nutrition. 68 (1998): 538–544.
8A. S. Raghuwanshi, S. S. Joshi, and S. Christakos, “Vitamin D and Multiple
Sclerosis,” Journal of Cellular Biochemistry 105, no. 2 (2008): 338–343; M. F.
Holick and T. C. Chen, “Vitamin D Deficiency: A Worldwide Problem with
Health Consequences,” American Journal of Clinical Nutrition 87, no. 4
(2008): 1080S–1086S; A. Ascherio, K. L. Munger, and K. C. Simon, “Vitamin
D and Multiple Sclerosis,” Lancet Neurology 9, no. 6 (2010): 599–612.
9American Egg Board, Egg Science and Technology Lesson Plan.
10Ibid.
11Ibid.
12Ibid.
13Mine and Yang, “Eggs,” 26.1–26.20.
14American Egg Board, Egg Science and Technology Lesson Plan.
15Mine and Yang, “Eggs,” 26.1–26.20.
16American Egg Board, Egg Science and Technology Lesson Plan.
17Mine and Yang, “Eggs,” 26.1–26.20.
18American Egg Board, Egg Science and Technology Lesson Plan.
19Ibid.
20U.S. Food and Drug Administration, Department of Health and Human
Services, Code of Federal Regulations, Title 21: Food and Drugs; Part 131:
Milk and Cream.
21M. Walsh, D. McMahon, and S. E. Duncan, “Milk and Dairy Products,” in
Food Chemistry: Principles and Applications, Y. H. Hui, ed. (West
Sacramento, CA: Science Technology Systems, 2007), 19.1–19.23.
22Walsh, McMahon, and Duncan. “Milk and Dairy Products,” 19.1–19.23.
23Walsh, McMahon, and Duncan. “Milk and Dairy Products,” 19.1–19.23.
24P. A. Cotton et al., “Dietary Sources of Nutrients Among U.S. Adults, 1994 to
1996.” Journal of the American Dietetic Association 104, no. 6 (2004): 92–930.
25P. K. Whelton et al., “Effects of Oral Potassium on Blood Pressure: Meta‐
Analysis of Randomized Controlled Clinical Trials,” Journal of the American
Medical Association 277, no. 20 (1997): 1624–1632; L. J. Appel et al. for The
DASH Collaborative Research Group, “A Clinical Trial of the Effects of
Dietary Patterns on Blood Pressure,” New England Journal of Medicine
336 (1997): 1117–1124.
26Dairy Management, Inc., “Facts About Cheese: Ingredient Applications,”
www.innovatewithdairy.com (Rosemont, IL: Dairy Management, Inc.,
2009b).
27K. J. Burrington, “Dairy Ingredients in Bakery Products,” American Institute
of Baking Technical Bulletin 27, no. 3 (2005).
28H. D. Goff, “The Impact of Dairy Ingredient Functionality on the Properties
of Ice Cream,” AgroFOOD Industry Hi‐Tech 20, no. 4 (2009): 43–45.
29M. D. Eisner, H. Wildmoser, and E. J. Windhab, “Air Cell Microstructuring in
a High Viscous Ice Cream Matrix,” Colloids and Surfaces: Physicochemical
and Engineering Aspects 263, no. 1–3 (2005): 390–399.
30D. S. Reid and O. R. Fennema, “Water and Ice,” in Fennema’s Food Chemistry,
S. Damodaran, K. Parkin, and O. R. Fennema, eds. (Boca Raton, FL: CRC
Press, 2008), 17–82.
31Dairy Management, Inc., “Exploring the Functionalities and Applications of
Dry Dairy Ingredients,” Think Dairy College Curriculum Program, Food
Science Curriculum Enhancement (Rosemont, IL: Dairy Management,
Inc., 2000).
32Ibid.
c10.indd 194 1/14/2016 7:28:47 AM
CHAPTER 10 • Egg- and Milk-Based Foods • 195
33Ibid.
34Ibid.
35E. J. Pyler, “Dairy Products and Blends,” in Baking Science and Technology,
vol. 1, 3rd ed. (Kansas City, MO: Sosland, 1988).
36Dairy Management, Inc., “Exploring the Functionalities and Applications of
Dry Dairy Ingredients.”
37Dairy Management, Inc., “Facts About Cheese.”
38W. Dufresne, “The Evolution of a Dish: An Interpretation of Eggs Benedict,”
Innovations at wd~50, Savory Workshop, StarChefs.com, International
Chefs Congress, NY, 2008.
39Sarah Rosenberg and Christina Caron, “Wylie Dufresne, Egg Man, Keeping
wd~50 Afloat in Tough Economy: Why Dufresne Adores Eggs,” retrieved
April 10, 2009, from http://abcnews.go.com/Nightline/Platelist.
40Dean Sommer, “Restricting Melt for Performance and Value,” Food Product
Design (December 2008).
c10.indd 195 1/14/2016 7:28:47 AM
http://abcnews.go.com/Nightline/Platelist
11
196 • CULINOLOGY®
Lead Author: James R. Adams, CCS®, CFS , Director of Process Optimization, Tyson Prepared
Foods Operations
Charles Hayes , CRC®, CEC , Director of Research and Development, National Accounts,
JMH Premium
Fermentation
Introduction
Culinology® denotes a blending of culinary arts and food
science; fermentation has long been regarded the same way.
In most cases, it refers to the conversion of carbohydrates
(also known as fermentable substrates ) under anaerobic
(without oxygen) conditions into carbon dioxide gas, organic
acids, and alcohol by the actions of microorganisms, primar-
ily bacteria and yeast. Two major exceptions occur: when
vinegar is produced from ethanol, and when molds (aerobic
organisms) are involved, as in mold‐ripened cheeses. In both
of these cases, oxygen is required. Many types of microorgan-
isms, alone or in combination, act on an assortment of fer-
mentable substrates to produce the vast number of fermented
foods and beverages consumed around the world every day.
These include well‐known alcoholic beverages such as beer,
ale, wine, and most of the hard liquors, as well as pickles, sau-
erkraut, pepperoni, yogurt, cheeses, yeast‐raised breads, and
more. In addition to its major impact on the flavor, aroma,
appearance, digestibility, and nutritional profile of foods, fer-
mentation is also one of the oldest methods of food preserva-
tion, dating to prehistoric times.
History of
Fermentation
It will never be known exactly when the first fermentation
took place, but most of the scientific community accepts the
premise that alcoholic fermentation was the first chemical
reaction ancient humans observed. This is known as the
Paleolithic hypothesis , a set of postulates or suppositions
attempting to explain the origin of fermentation in pre‐
history. 1 In 2004, it was confirmed by chemical analysis of
ancient organic compounds that were absorbed and pre-
served in pottery jars that a mixed fermented beverage of
rice, honey, and fruit was produced as early as 9000 years ago
in the area known today as China.
The earliest evidence of yeast fermentation used to con-
vert the natural fruit sugar in grapes to alcohol was found in
the Middle East and dates back 8000 years. The ancient
Mesopotamians and Sumerians were brewing as early as
10,000 bc. However, Babylonian clay tablets with a recipe
for beer, from approximately the year 6000 bc, provide the
first documented evidence of beer‐making. This recipe used
underbaked bread made from germinated barley as the
yeast source. When the bread was cut into small pieces and
placed in a large jug with water, the yeast fermented the
malt produced by the barley (as takes place in “malting” in
the brewing process) when the dough was par‐baked.
This pre‐inoculated malt‐water fermented into a kind of
beer. The common people considered this beverage ready to
drink; however, the elite usually filtered it first. Dates, herbs,
and honey were sometimes added for flavoring. It is inter-
esting to note that early beer formulas did not include hops,
which were not introduced until centuries later. As a matter
of fact, the beer of that period, while classified as such, does
not resemble anything we know as beer today.
Ancient Egyptian writings and drawings found on the
walls of tombs indicate that fermentation was used to pre-
serve vegetables, including cucumbers, circa 3000 bc. 2 It was
widely known in ancient Egypt that Cleopatra believed con-
suming fermented vegetables greatly enhanced her beauty. 3
The Hebrew Bible refers to yeast‐leavened bread. Evidence
found on Egyptian tombs suggests that using starter dough
(containing yeast from a previous batch) was a common prac-
tice. That continues today, notably in San Francisco sour-
dough bread manufacture. Breads made from a starter have a
distinctive aroma and sour flavor and are leavened naturally.
Archaeological evidence from other cultures indicates that
a similar process was used to make dough rise; a beer‐type
liquid—the frothy residue of the brewing process—was added
c11.indd 196 1/22/2016 1:11:54 PM
CHAPTER 11 • Fermentation • 197
to dough. This liquid contained the yeast that had been used
in the alcoholic fermentation process and acted as a starter
culture for bread‐making.4
Evidence of milk fermentation has been found in the area
of ancient Babylon (a fermentation hot spot!) from circa
3000 bc.5 It more than likely developed when milk soured or
started to form curds caused by naturally occurring bacteria
converting lactose (milk sugar) into lactic acid, or by rennin
(also known as chymosin), a naturally occurring enzyme
found in the stomach lining of calves that causes milk protein
(casein) to coagulate and produce a sweet curd. Rennin is still
used in cheese‐making.
As the frequency of these experiences increased over the
millennia, the art of fermenting foods and beverages evolved
into a better understanding of wine‐making, brewing, pick-
ling, and bread‐making. Early humans’ limited knowledge of
the fermentation arts was passed down through centuries of
practitioners, called artisans. This slow and deliberate trans-
fer of knowledge, along with repeated observation, led ulti-
mately to our scientific understanding of the fermentation
process, thousands of years later.
The gradual progression of the fermentation arts contin-
ued without scientific understanding until Louis Pasteur,
a French chemist, connected fermentation to yeast in 1854.
Until then, it was believed that fermentation was a result of
chemical activity and changes brought about by enzymes,
and did not involve any living organisms. In 1680 Antonie van
Leeuwenhoek, the inventor of the microscope, first observed
yeast cells, which he regarded as “inanimate spheres,” as he
saw no apparent movement or other indication they were
alive. Pasteur’s work proved the existence of living microbes
was integral to the fermentation process. He called these
microbial agents ferments. Using a microscope, he observed
that the same ferments were present each time he allowed
fermentation of sterilized sugar water inoculated with sedi-
ment from previous wine fermentations. Pasteur is quoted in
the research paper “Memoire sur la fermentation alcoolique”: “I
have attempted to prove that brewer’s yeast placed into sugar
water lives . . . I am of the opinion that alcoholic fermentation
never occurs without simultaneous organization, develop-
ment, and multiplication of cells.”6
Fermentation was now much more than just an art: It was
a science. The science of fermentation continues today and is
called zymology.
Preservation of
Perishable Products
by Fermentation
According to the Food and Agriculture Organization of the
United Nations (FAO), approximately 800 million people
around the world do not have enough food to eat. This num-
ber becomes much larger, approximately 1.2 billion, if the
group “not free from hunger” is included. When one takes
into account that over 40 percent of available food ends up as
waste in the United States alone, it is vitally important to find
a way of turning this loss into edible food. Fermentation is
one answer to the problem. It is a cheap and energy‐efficient
means of preserving perishable raw materials, especially
freshly harvested fruits and vegetables. Fermentation is via-
ble on a small scale and does not require extensive knowl-
edge of or the equipment needed for canning, refrigeration, or
freezing, or any of the more capital‐ and knowledge‐intensive
methods we take for granted in the more developed world.
Fermentation can provide safe and nutritious food products,
made using relatively simple and affordable means, that can
supply the nutritional staples absent in marginalized and
vulnerable cultures.7
The aim of food preservation is to treat perishable food in
such a way as to increase its shelf life by slowing or stopping
the natural process of spoilage or decay, and to render the
food safe and nutritious for human consumption. The
method employed depends on the type of food to be pre-
served and on whether changes in the quality and sensory
attributes resulting from the preservation process are accept-
able. In many cultures, fermentation is coupled with drying
as a preservation technique.
Fermentation can also utilize foods otherwise considered
waste, such as fish heads and intestines (and other viscera)
and press‐cakes left from the processing of other foods—such
as soy pulp left over from making tofu, peanut press‐cake from
peanut oil production, and coconut press‐cake from coconut
milk extraction—and convert them into edible food by chang-
ing their consistency, flavor, and texture to produce a product
that is more palatable and digestible. Even ground bones are
fermented and used to make survival food (dodery) in Sudan.
In addition to the more exotic subsistence foods eaten
around the world, foods that are a standard part of the
American diet, such as bread, butter, cheese, yogurt, pepper-
oni, pickles, olives, sauerkraut, wines, beers, coffee, tea, black
pepper, and soy sauce, and many more are all prepared to
some degree through the process of fermentation.
Process of
Fermentation
While fermentation seems a simple way to preserve food, it
actually encompasses the interaction and understanding of
many scientific disciplines, including microbiology, biochem-
istry, bioengineering, genetics, agriculture and horticulture.
Fermentation microorganisms can be divided into five
distinct groups:
1. lactic acid–producing bacteria
2. acetic acid–producing bacteria
3. alkaline‐fermenting bacteria
4. fungi
5. alcohol‐producing yeast and mold
All of these are highly specialized catabolic organisms,
meaning they alter organic material just enough to facilitate
c11.indd 197 1/13/2016 7:54:51 AM
198 • CULINOLOGY®
FIGURE 11.1 Fermented foods from around
the world.
Name and Region
Type of
Product
Indian subcontinent
Acar, Achar, Tandal achar, Garam
nimboo achar
Pickled fruits
and vegetables
Gundruk Fermented
dried
vegetables
Lemon pickle, Lime pickle, Mango pickle
Southeast Asia
Asinan, Burong mangga, Dalok, Jeruk,
Kiam‐chai, Kiam‐cheyi, Kong‐chai, Naw‐
mai‐dong, Pak‐siam‐dong, Paw‐tsay,
Phak‐dong, Phonlami‐dong, Sajur asin,
Sambal tempo‐jak, Santol, Si‐sek‐chai,
Sunki, Tang‐chai, Tempoyak, Vanilla
Pickled fruits
and vegetables
Bai‐ming, Leppet‐so, Miang Fermented tea
leaves
Nata de coco, Nata de pina Fermented fruit
juice
East Asia
Bossam‐kimchi, Chonggak‐kimchi,
Dan moogi, Dongchimi, Kachdoo
kigactuki, Kakduggi, Kimchi, Mootsanji,
Muchung‐kimchi, Oigee, Oiji, Oiso baegi,
Tongbaechu‐kimchi, Tongkimchi, Totkal
kimchi
Fermented in
brine
Cha‐ts’ai, Hiroshimana, Jangagee, Nara
senkei, Narazuke, Nozawana, Nukamiso‐
zuke, Omizuke, Pow tsai, Red in snow,
Seokbakji, Shiozuke, Szechwan cabbage,
Tai‐tan tsoi, Takana, Takuan, Tsa Tzai,
Tsu, Umeboshi, Wasabi‐zuke, Yen tsai
Pickled fruits
and vegetables
Hot pepper sauce
Africa
Fruit vinegar Vinegar
Hot pepper sauce
Lamoun makbouss, Mauoloh, Msir,
Mslalla, Olive
Pickled fruits
and vegetables
Oilseeds, Ogili, Ogiri, Hibiscus seed Fermented
fruit and
vegetable
seeds
Wines Fermented
fruits
Name and Region
Type of
Product
Americas
Cucumber pickles, Dill pickles, Olives,
Sauerkraut
Pickled fruits
and vegetables
Lupin seed, Oilseeds Pickled oilseed
Vanilla, Wines Fermented
fruits and
vegetables
Middle East
Kushuk Fermented
fruits and
vegetables
Lamoun makbouss, Mekhalel, Olives,
Torshi, Tursu
Pickled fruits
and vegetables
Wines Fermented
fruits
Europe and World
Mushrooms, Yeast Molds
Olives, Sauerkohl, Sauerruben Pickled fruits
and vegetables
Grape vinegar, Wine vinegar Vinegar
Wines, Citron Fermented
fruits
Source: Food and Agriculture Organization of the United Nations.
growth, not completely metabolize the material. This break-
ing down of complex molecules to simpler molecules results
in energy release used by the microorganisms for growth.
Important factors that affect microbial growth are salinity
(percent salt), acidity (pH), temperature, water activity
(moisture available to sustain growth of microorganisms),
and the amount of oxygen present.
Different fermentation microbes utilize oxygen differently
and are characterized into specific groups. Aerobic microor-
ganisms, such as some bacteria and all molds, thrive in the
presence of oxygen. Anaerobic microorganisms, such as many
bacteria, thrive in the absence of oxygen. Facultative anaer-
obes, such as yeast, can thrive in the presence or absence of
free oxygen (but produce alcohol and carbon dioxide only via
anaerobic respiration). Micro‐aerophilic organisms can grow
in the presence of minute amounts of free oxygen.8
Biochemical pathways associated with fermentation begin
with the dissimilation, or breakdown, of sugar, in most cases
glucose. This process, known as glycolysis, is a series of ten
steps (known as the Emden‐Meyerhof pathway) that convert
one molecule of glucose to two pyruvate molecules. Although
dissimilation can take place along several pathways, the
Emden‐Meyerhof pathway is the one most representative of (continued )
FIGURE 11.1 (continued)
c11.indd 198 1/13/2016 7:54:51 AM
CHAPTER 11 • Fermentation • 199
lactic, alcohol, and acetic acid fermentations.9 Pyruvate serves
as the junction in the glycolysis cycle where either lactic acid
or ethanol can be produced, depending on the type of organ-
ism responsible for the fermentation. As glycolysis continues,
pyruvic acid is formed. Lactic acid bacteria secrete enzymes
that reduce the pyruvic acid to lactic acid, while yeasts reduce
the pyruvic acid to alcohol. Acetobacter bacteria oxidize the
alcohol into acetic acid (the primary acid in vinegar).
Lactic Acid Bacteria
By far the largest groups of fermented foods and beverages
are produced through lactic acid bacterial fermentation.
Lactic fermentations are used to produce summer sausage,
pickles, sauerkraut, olives, yogurt, some cheeses, cultured
butter, and cultured buttermilk. Although several bacteria
are closely associated with lactic acid fermentations, the
most important is Lactobacillus, a rod‐shaped gram‐posi-
tive bacterium. (Gram staining is a primary method for dif-
ferentiating bacterial strains into two distinct families:
gram‐positive and gram‐negative.) Lactobacilli, naturally
occurring bacteria widely distributed in animal feeds,
manure, milk, and milk products, can be grown into pure
cultures for industrial use in a variety of food applications.
These starter cultures may be stored frozen in liquid nitro-
gen or freeze‐dried for convenience.
glucose
glucose-6-phosphate
fructose-6-phosphate
fructose-1,6-diphosphate
glyceraldehyde-3-phosphate glyceraldehyde-3-phosphate
ATP
ADP
ATP
ADP
1,3-diphosphoglyceric acid
3-phosphoglyceric acid
2-phosphoglyceric acid
phosphoenolpyruvic acid
pyruvic acid
–H2O
+Pi
ADP
ATP
ADP
ATP
NAD
NADH2
1,3-diphosphoglyceric acid
3-phosphoglyceric acid
2-phosphoglyceric acid
phosphoenolpyruvic acid
pyruvic acid
–H2O
+Pi
ADP
ATP
ADP
ATP
NAD
NADH2
FIGURE 11.2 Embden‐Meyerhof pathway.
Source: Dr. Kenneth Todar, University of Wisconsin.
glucose
2 phosphoglyceric acid 2 triose phosphate
2 pyruvic acid 2 lactic acid
fructose diphosphate(a) Lactobacillus
2 ADP2 ATP
2 ADP
2 ATP
2 NAD2 NADH2
(b) Saccharomyces glucose
2 phosphoglyceric acid 2 triose phosphate
2 pyruvic acid 2 alcohol
fructose diphosphate
2 ADP2 ATP
2 ADP
2 ATP
2 NAD
2 CO2
2 NADH2
FIGURE 11.3 Bacteria and yeast pathway.
Source: Dr. Kenneth Todar, University of Wisconsin.
c11.indd 199 1/13/2016 7:54:51 AM
200 • CULINOLOGY®
Lactobacillus plantarum is a homo‐fermentative bacte-
rium, meaning it produces lactic acid and little or no other
by‐products (including no carbon dioxide gas). Lactobacillus
brevis is characterized as hetero‐fermentative because it pro-
duces lactic acid along with large amounts of carbon dioxide
gas. Most fermentations use homo‐fermentative strains be-
cause the intent is to produce as much lactic acid as possible
(to preserve the food by lowering the pH) in a relatively short
time. Carbon dioxide gas may be detrimental to the product
being fermented, as will be seen in the discussion on cucum-
ber pickle fermentation.
L. plantarum is commonly used in the production of fer-
mented dried meats, pickles, and sauerkraut, producing the
largest amount of lactic acid and giving these foods their char-
acteristic tangy taste. L. brevis is one of the main organisms
used to produce kimchi, the Korean version of sauerkraut.
L. mesenteroides is a hetero‐fermentative halophilic (salt‐
tolerant) bacterium known for its role in the fermentation of
fresh cabbage to sauerkraut. Because it is hetero‐ fermentative,
like L. brevis, it metabolizes sugar to carbon dioxide in addi-
tion to lactic acid. L. mesenteroides is the organism of choice
for sauerkraut fermentation because it tolerates the high salt
concentration used in this process. In making sauerkraut,
raw cabbage is dry‐salted in layers within the fermentation
tank to dehydrate (draw water out of ) the cabbage cells by
osmosis; this creates a concentrated salt brine.
In the case of cucumber pickles, a concentrated salt brine,
5 to 7 percent weight/volume, is added to fresh cucumbers. It
is several days before the brine becomes diluted to around
4 percent salt due to equalization with water coming from
the fresh cucumbers, which contain no salt. In both cases, the
salt level is at least inhibitory, if not downright toxic, to most
bacteria other than L. mesenteroides.
Another lactic acid bacterium commonly used in com-
mercial applications is Pediococcus cerevisiae, a homo‐
fermentative, sphere‐shaped, gram‐positive bacterium.
P. cerevisiae is one of the predominant organisms used in
fermenting meats like sausage, pepperoni, and salami.
Lactic acid bacterial growth follows a well‐studied and
understood pathway known as the bacterial growth curve,
which is divided into four phases:
1. Lag phase: The lactic bacteria are becoming accustomed
to their new environment and are starting to metabolize
sugars.
2. Log phase: The bacteria undergo exponential growth due
to binary fission. Large amounts of lactic acid are pro-
duced along with by‐products such as carbon dioxide gas,
acetic acid, and alcohol, depending on the microorganism
involved.
3. Stationary phase: The bacteria use up most of the nutrients
in their surroundings (now contaminated with a buildup
of their waste products) and stop reproducing.
4. Death phase: All growth ceases, and the microorganisms
die off rapidly from a combination of lack of nutrients and
a buildup of toxic waste.
Lactic Fermentations: Pickles
First, it is important to understand the pickle category.
Pickled cucumbers are produced via three preservation
methods, and the corresponding products exhibit signifi-
cantly different attributes. The three methods are fermenta-
tion pickles (referred to in the pickling industry as processed),
fresh pack, and overnight/refrigerated. In terms of market
share, fermentation pickles are the largest category, with
approximately 56 percent of the market. They are popularly
used as hamburger slices for the foodservice business. Fresh‐
pack pickles are the next largest segment, with approximately
24 percent of the cucumber crop. These pickles are packed as
fresh cucumbers in flavored salt brine and then pasteurized
to render the product commercially sterile. Overnight/refrig-
erated pickles are packed fresh in seasoned brine and then
immediately refrigerated for warehousing and storage. This
type of pickle is sold in the refrigerated case of grocery stores
and delis. They are also called deli dills (full sour and half
sour), and they have been sold in delicatessens for over 100
years, especially in larger U.S. cities, like New York and
Chicago, with substantial Jewish‐German and Polish popula-
tions. These pickles comprise approximately 20 percent of
pickle sales. According to data compiled by Pickle Packers
International, a trade association for the pickled vegetable
industry, U.S. consumers eat over 2 billion pounds of pickles
each year, or about 9 pounds per person.10 Here we examine
the first category: fermented pickles.
Fresh cucumbers are harvested by hand or by machine.
Once harvested, they are transported to the processing site,
where they are inspected, sized, and loaded into fermenta-
tion tanks at a pre‐determined percent ratio of solids to liq-
uids. Most often this ranges from 60:40 to 70:30, where the
larger number represents the percent of solids (pounds of
cucumbers) per tank. The liquid portion of the ratio is brine
made up of salt, water, and calcium chloride, which is used to
prevent enzymatic softening of the cucumbers during the
fermentation.11
The salt concentration approximates 5 percent, or 19–20
degrees Salometer. The concentration of salt in the brine
is expressed as degrees Salometer, which represents the
LOG
PHASE
STATIONARY
PHASE
DEATH
PHASE
LAG
PHASE
Time in Hours
L
O
G
N
U
M
B
E
R
FIGURE 11.4 Bacterial growth curve.
Source: James Adams.
c11.indd 200 1/13/2016 7:54:51 AM
CHAPTER 11 • Fermentation • 201
saturation level of sodium chloride by weight. Salometer
readings range from 0 to 100, where a reading of 100 equals
100 percent saturation of sodium chloride by weight (this is
equal to 26.4 percent concentration of sodium chloride in
water). During the fermentation, Salometer readings are
taken at regular intervals to ensure the brine stays at the
proper salt concentration.
Pickling from the early 1900s through the late 1970s used
authentic redwood or cypress tanks. Over long periods, these
tanks began to leak brine, contributing to problems with con-
tamination of salt brine into ecologically sensitive areas. The
solution to the problem has been to replace wood with tanks
made of fiberglass.
Because cucumbers are buoyant, they must be kept sub-
merged by using a device called a head. The head is held in
place by anchors that are attached to the tanks. After the
tank is filled and headed, as part of the controlled fermenta-
tion process chlorine is added to the tank to stop the growth
of competitive organisms (especially coliform bacteria).
Within 18 hours, glacial acetic acid (25 times the acidity of
white table vinegar) is poured into the tank to adjust the pH
of the brine to ideal growth conditions. L. plantarum starter
culture is added next, usually within 12 hours. The pure cul-
ture added is a homo‐fermentative bacterium used to limit
the production of carbon dioxide and build acid quickly. Pure
culture fermentation allows the L. plantarum, which is highly
concentrated in the pure culture media, to outrun the natural
bacteria, which are predominantly hetero‐fermenters (gas
producers), which may be present in the fermentation.
The fermentation starts slowly and then becomes more
vigorous in the next two to three days, depending on the tem-
perature of the brine (in colder climates, the brine must be
heated to prevent a stuck fermentation). The optimum brine
temperature is found by consulting the supplier of the starter
culture or reference books on microbiology, such as Bergey’s
Manual of Determinative Bacteriology.12
The tanks are monitored daily to ensure the fermentation
is following the bacterial growth curve and all the residual
sugar in the cucumbers is converted to lactic acid. Measuring
turbidity of the brine is a quick qualitative method of assess-
ing the progress of fermentation. This is done by placing a
sample tube of brine against an embossed chart with four
lines; the top line, number 1, is the thinnest line, and the bot-
tom line, number 4, is the thickest and boldest line on the
card. The technician records the lowest number line that can
easily be read through the brine. At the start the brine is clear,
thin, and without cloudiness. As the fermentation advances,
the brine becomes opaque and starts to thicken slightly. As
the fermentation gets more active, the brine’s opacity
increases and even the bottom line cannot be seen. At this
point, the brine is recorded as 4+, indicating a vigorous fer-
mentation is taking place. Most fermentations are completed
within one to two weeks, depending on the temperature.
Carbon dioxide produced as a result of hetero‐fermentation
can become supersaturated in the brine if not removed by
mechanical means (purging). In purging, the brine is scrubbed
of accumulated gas by injecting nitrogen (an inert gas) into the
tank; this causes the carbon dioxide gas to rise to the tank’s
surface and release into the atmosphere, thus preventing its
infiltration into the cucumbers, which would make them taste
fizzy (like soda). Purging was developed in the late 1970s by
scientists and engineers at Michigan State University working
with researchers at the Agricultural Research Station, part
of North Carolina State University’s agricultural extension
service.
After cucumber pickles are fermented and the residual
sugar completely converted, they are de‐salted and further
processed into sweet gherkins, hamburger chips, dill pickles,
or sweet and dill relish.
Acetic Acid Bacteria and the
Production of Vinegar
Acetic acid bacteria are able to convert ethanol (drinking or
grain alcohol) into acetic acid and are commonly known as
acetobacters. Acetobacter aceti is the most common of the
½” Gap to Allow CO2 to Escape
4”X4” Cross Tie – Perpendicular to
Head Boards to Hold Head in Place
Pump Box – Allows Circulation of
Brine and Side Arm Purging
Hold Down Hooks – Metal Hooks
That Hold Cross Ties in Place
Tank Hoops – Metal Bands That
Hold Tank Together
Wooden
Fermentation Tank
Head Boards to Hold Cucumbers
Under Brine
FIGURE 11.5 Open‐top cucumber fermentation
tank.
Source: James Adams.
Tank – Fabricated out of
Blown Fiberglass
“Closed”
Fermentation Tank
Perforated Plastic to Hold
Cucumbers Under Brine
Cucumbers Under Brine
Brine Surface
Brine Sample Tube – ¾” PVC pipe
FIGURE 11.6 Closed‐top cucumber fermentation
tank.
Source: James Adams.
c11.indd 201 1/13/2016 7:54:53 AM
202 • CULINOLOGY®
organisms used in the production of vinegar. They are gram‐
negative, rod‐shaped, aerobic bacteria that derive energy
from the oxidation of ethanol to acetic acid. This is a
two‐stage process. The first stage occurs when yeast
(Saccharomyces cerevisiae) is added to a fermentation tank
containing a sugar solution (most commonly fructose,
derived from cane or corn sugar) at 80 to 85°F (27 to 29°C),
the optimum growth temperature. The yeast are able to
convert approximately 90 percent of the sugar into alcohol.
The remaining 10 percent are volatile components, such as
aldehydes, ketones, and esters, which are removed via dis-
tillation if the intent is to produce purified spirits. However,
in the commercial production of vinegar, acetobacter bac-
teria (available as a starter culture known as the mother of
vinegar) are added and the liquid vigorously aerated, allow-
ing the bacteria to grow and oxidize the alcohol to acetic
acid. The yeast and bacteria coexist in the tank in a biologic
relationship known as commensalism, where two organ-
isms live in close proximity with at least one benefiting by
the other’s presence. In vinegar‐making, the acetobacter
bacteria depend on the alcohol produced by the yeast as a
substrate for conversion to acetic acid.13
This type of vinegar contains 4 to 5 percent acetic acid and
is referred to as 40–50 grain. Grain is calculated by multiplying
the percent acetic value (obtained through titration against a
known concentration of sodium hydroxide solution) by 1000.
Common white vinegar is approximately 40 grain. Industrial
vinegar used in food manufacture is 120 grain, or 12 percent
acetic, and is manufactured by the distillation of naturally fer-
mented white vinegar. It must be diluted in the final product
in order to meet FDA labeling guidelines. According to the U.S.
Food and Drug Administration (FDA), there is no standard of
identity for vinegar; however, for labeling purposes all vine-
gars used in foods must contain 4 percent acetic acid, which
must be prepared by the alcoholic and subsequent acetous
fermentations of whichever fruit or grain the vinegar is made.
Glacial acetic acid has a value of 1050 grain and is pure acetic
acid. While purified acetic acid is itself safe for use in foods, the
FDA says, “This Administration, in the enforcement of the
Federal Food, Drug, and Cosmetic Act, has maintained the posi-
tion that diluted acetic acid is not a vinegar of any kind and has
advised against its use in food products customarily expected
to contain vinegar. When used as an ingredient in food, it should
be declared by its name, acetic acid or diluted acetic acid.” Glacial
acetic acid is used primarily as a reagent for the production of
chemical compounds. Today, most industrial production is
synthetic, using the BP Cativa process, involving methanol
(wood alcohol) and carbon monoxide reacted together at high
temperatures and under extremely high pressure.
Most vinegars are filtered to improve clarity and pasteur-
ized to prevent potential outgrowth problems during ware-
housing or storage. It is important to note that if the vinegar
bottle’s hermetic seal is broken, the vinegar can become oxi-
dized to water and carbon dioxide.
$0
$20,000,000
$40,000,000
$60,000,000
$80,000,000
$100,000,000
$120,000,000
White Distilled
Vinegar
Apple Cider
Vinegar
Malt Vinegar Balsamic Vinegar Wine Vinegar Rice Vinegar
Calendar Year 2008
Calendar Year 2009
Calendar Year 2010
Calendar Year 2011
CURRENT 52 WEEKS THRU Jan. 2013.
Vinegar by $ Sales
FIGURE 11.7 Vinegar by dollar sales.
Source: The Vinegar Institute (data obtained from Nielsen), www.versatilevinegar.org.
c11.indd 202 1/13/2016 7:54:54 AM
CHAPTER 11 • Fermentation • 203
The type of vinegar produced depends on the substrate
being fermented. The substrate is the substance being acted
on by microorganisms or enzymes—in some cases, both.
White vinegar uses grain alcohol as the substrate. Fruit vine-
gars are produced by fermenting apples or other fruits, but
cider vinegar can only be prepared from “the juice of apples,”
according to the FDA. Some exotic fruit vinegars are pro-
duced in Asia—for example, persimmon, yam, and black
plum. Rice vinegars are also an Asian specialty. Fruit‐flavored
vinegars are produced by adding fruit flavors (and colors) to
white vinegar. Malt vinegar is made from malted barley and is
especially popular in England, where it is used liberally to fla-
vor fish and chips. Wine vinegar is made from red or white
wine and is less acidic than regular white vinegar, as the wine
used as a substrate contains less alcohol than grain alcohol.
Some upscale varietal wine vinegars are also available, includ-
ing sherry vinegar and champagne vinegar. Balsamic vinegar
(commonly from Modena, Italy) is made by aging vinegar, fer-
mented from white grapes, in wooden casks for 3 to 12 years
or longer. The older vintages are extremely concentrated and
expensive. Balsamic is unlike other wine vinegars in that
there is no starting alcohol fermentation before the acidifica-
tion begins. The fermentation process converts the grape
must ( juice of just‐harvested white Trebbiano grapes, boiled
down to approximately 30% of the original volume to create a
concentrate) into alcohol and acid at the same time using a
mixture of yeast and bacterial cultures.14
In a 2007 report presented at the 2007 annual meeting
of the Vinegar Institute (an international trade association
managed by the Kellen Company [www.versatilevinegar
.org]), the Nielsen Company noted that white distilled vine-
gar has 68 percent of the unit share of vinegars. Cider vinegar
accounts for 20 percent, and specialty vinegars account for
12 percent. Of the specialty vinegar category, 39 percent is red
wine, 30 percent balsamic, 13 percent all other wine, 12 per-
cent rice vinegar, and 6 percent all others.
According to the Vinegar Institute, although bottled vine-
gar makes up the majority of sales at the retail level (33.7 per-
cent), it is also a key ingredient in a number of foods used
daily in the United States. These foods include dressings and
sauces (16.8 percent), pickles (14.8 percent), mustard (11.5
percent), and other processed foods including tomato prod-
ucts such as salsa, ketchup, and pasta sauces (23.2 percent).
Alkaline Fermenters
Although alkaline fermenters are important in the produc-
tion of fermented foods around the world (especially in Third
World countries), the number of foods produced using this
method pales in comparison to the vast number produced by
lactic or acetic acid fermentations. Bacillus licheniformis,
Bacillus pumilis, and Bacillus subtilis (most predominant) are
associated with this type of fermentation. All are gram‐posi-
tive spore‐forming rods. These bacteria can hydrolyze, or
split protein into amino acids and peptides, with ammonia as
a by‐product. As the concentration of ammonia builds, the
pH of the material is raised above 7.0 (as high as 9.0 to 11.0
pH), inhibiting the growth of spoilage organisms.15
Thua‐nao and kinema are two examples of alkaline‐
fermented foods from Thailand that are made from cooked
soybeans. Dawadawa, from Africa, is made from fermented
locust beans. Puto, from the Philippines, is an alkaline‐
fermented poultry egg product. Natto is a popular fermented
soybean product from Japan. All of these are extremely pun-
gent and slimy due to the putrefactive end‐products of
proteolysis (breakdown of proteins).
Molds
Molds are aerobic, filamentous organisms that are salt‐
tolerant members of the fungi family. Molds have tubules
(filaments), called hyphae, that grow and intertwine. As these
tubules clump together, they form a mass of cells known as
mycelia. Molds are visible without magnification, unlike bac-
teria and yeasts, and they can reproduce sexually or asexually.
They are used in fermentation of vegetable products (a good
example is tempeh, a mold‐fermented soybean cake of
Indonesian origin; another is the popular Japanese condi-
ment and soup base miso), dairy (particularly mold‐ripened
cheeses such as bleu, Camembert, and Brie), and meat prod-
ucts (especially dry‐fermented sausages).
Yeast
Yeast are single‐celled facultative (able to live in the presence or
absence of oxygen) microorganisms that are members of the
fungi kingdom, along with mushrooms and molds. They can
convert sugar to alcohol and carbon dioxide gas, but only under
anaerobic conditions. Under aerobic conditions, yeast simply
make more yeast! They are the primary engines in the produc-
tion of beer, wine, and alcoholic spirits as well as leavened breads.
Yeast anaerobically convert one glucose molecule into two
ethanol molecules and two carbon dioxide molecules:
C H O C H OH CO6 12 6 2 5 22 2
1 2 2glucose ethanol carbon dioxide
Both alcohol and carbon dioxide are produced during fer-
mentation; however, the alcohol flashes off during baking,
and the carbon dioxide bubbles dissipate out of wine but are
trapped in beer.
Alcohol Production
It may be difficult to see the similarities of beer, wine, and liq-
uor (and other alcoholic beverages), but they all start off the
same way: the fermentation of carbohydrate substrates
through the actions of yeast. Yeast is ubiquitous in our environ-
ment. Because yeast spores are widely dispersed throughout
the environment (including on the surface of grapes and other
fruits and vegetables, and even in the air we breathe), fermen-
tation occurs in nature all the time. The art of brewing and
winemaking is the controlled application of fermentation to
selected raw ingredients in order to produce alcoholic bever-
ages with specific flavors, colors, aromas, and alcohol levels.
The production of alcohol occurs in the absence of oxygen,
which inhibits the growth of competing aerobic microorgan-
isms in the fermentation tank. However, from the yeast’s
c11.indd 203 1/13/2016 7:54:54 AM
204 • CULINOLOGY®
point of view, alcohol and carbon dioxide are waste products,
and as the yeast continues to grow and metabolize, the accu-
mulation of alcohol becomes toxic when it reaches a concen-
tration between 14 and 18 percent, killing the yeast cells. This
is the reason the maximum alcohol achievable in wine and
beer cannot exceed 18 percent. To produce higher concentra-
tions of alcohol, the fermented products must be distilled to
remove excess water. Beer and wine made and sold in the
United States are highly regulated by both the federal and
state governments. The maximum allowable percentage of
alcohol in each beverage varies by state.
Generally speaking, any alcoholic beverage created from
fermenting fruit juice can be called wine (hard cider notwith-
standing). However, commercially speaking, wine is fer-
mented grape juice from the wine grape, Vitis vinifera. Other
wines are specifically referred to by the name of the fruit from
which they are fermented—for example, elderberry wine and
blueberry wine. Other wines, such as dandelion and honey
wine (mead), are not derived from fruit juices at all.
Beer, as developed in Western cultures and as it is most
commonly recognized today, is created from the fermenta-
tion of malt, which comes from the digestion of germinated
barley grains. Many other grains (such as wheat, rice, millet,
and sorghum) are used in other brews and cultures. In the
United States, these alternative grains are occasionally seen
in specialty microbrews, where the artisanal brewer exercises
poetic license to create unique and distinctive flavors.
Besides the substrate being fermented, the biggest differ-
ence between beer and wine is the fermentation process and
how it is manipulated to produce the desired result. Brewing
beer is essentially a steeping process, like making tea, where
the ingredients (malted grains and hops) are soaked in water
(steeped) and then filtered out. Selected brewer’s yeasts are
added to the filtered liquid (called wort), and fermentation
begins. The wort is like a beer starter in that it is unfermented
beer containing malt extract and water and is used in combi-
nation with yeast to start the fermentation. Winemaking, on
the other hand, involves crushing the entire fruit (typically
grapes) and fermenting the juice that results.
Beer‐Making Ingredients
The basic ingredients of beer are water; a fermentable (con-
vertible into alcohol) sugar source, such as malted barley; a
brewer’s yeast, to produce the fermentation; and a flavoring,
such as hops. Beer originated as a flat, slightly sweet malt‐
and grain‐flavored beverage that spoiled quickly. In the eighth
century, however, brewers in central Europe found the addi-
tion of hops flowers preserved the beer and gave it a more
palatable, slightly bitter taste. Even with hops, early beer was
not necessarily pleasant‐tasting by our standards. It became
the beverage of choice, however, due to the scarcity of good‐
quality drinking water, not to mention the buzz generated by
the alcohol.
Hops are the female flower clusters (commonly called seed
cones or strobiles) of the hop species, Humulus lupulus. They
are used primarily as a flavoring and stabilizing agent in beer,
to which they impart a bitter, tangy, often citrus‐like flavor
and aroma. Hops are used extensively in brewing to balance
the sweetness of the malt with bitterness; they contribute a
variety of desirable flavors and aromas and have an antibiotic
effect that favors the activity of brewer’s yeast over less desir-
able microorganisms.
Hops are normally dried in an oast house or hop kiln
before they are used in the brewing process. Hop resins are
composed of two main acids: alpha and beta acids. Alpha
acids are responsible for the bitter flavor in the beer and have
a mild antibiotic/bacteriostatic effect against gram‐positive
bacteria, which favors the activity of the brewer’s yeast in the
fermentation tank. Beta acids contribute to beer’s bitter
aroma, and high–beta acid hop varieties are often added at
the end of the wort boil for aroma.
When sourcing hops, a brewmaster seeks hops with a spe-
cific level of alpha acid. This is referred to as the alpha acid
percent, or AA percent, which is a measure of the percentage
of the flower’s weight composed of the alpha acid resin. This
is a key measure of bittering potential. All merchants provide
an AA percent to assist in selecting hops. That said, the
aroma hops provide comes from essential oils from hops high
in beta acids. The blending of the two types is where the art
of the brewmaster becomes essential.
Water is another key component in brewing beer. It con-
stitutes more than 90 percent of beer and has a natural effect
on how the beer ferments and tastes. Levels of dissolved min-
eral ions in the water affect the activity of several starch‐
degrading enzymes in malt. The levels of these ions directly
affect the water’s pH, which in turn affects the enzyme’s effec-
tiveness in converting starch to maltose, the primary sugar
the yeast uses to ferment. Having a correct ion balance greatly
influences the extraction yield of the malt and the resulting
sensory attributes of the beer.
The calcium ion is by far the most influential mineral in the
brewing process. Calcium reacts with phosphates, lowering
the pH of the mash. This lowering of the pH is critical in that
it provides an ideal environment for starch enzymes alpha‐
amylase and beta‐amylase as well as proteolytic enzymes.
Other important mineral ions include magnesium, which is
most important as a yeast nutrient during fermentation;
sodium, which contributes to the perceived flavor of beer by
enhancing its sweetness; potassium, which creates a salty fla-
vor effect and is required for yeast growth; and phosphates,
which are important pH buffers in brewing and useful for
reducing the pH in mashing and during the hop boil. Calcium
and magnesium chlorides give body, palate fullness, and soft‐
sweet flavor to beer. On the other hand, the presence of car-
bonate ions raises the pH, resulting in less fermentable worts,
unacceptable wort color values, difficulties in wort filtration,
and less efficient separation of protein and protein‐tannin
complexes. They are to be avoided or reduced if at all possible.
Before brewing, the water is checked to make sure all min-
erals are in balance so the yeast performs to its maximum
potential.
In all cases, the fermentable substrate for beer‐making is
a carbohydrate. Fats and proteins cannot be fermented
c11.indd 204 1/13/2016 7:54:54 AM
CHAPTER 11 • Fermentation • 205
without the presence of a carbohydrate. Although it is pos-
sible to use any grain to make a beer‐like beverage, the most
commonly used fermentable substrate is barley, as most of
the other grains (corn, wheat, rice) are eaten more directly as
foods, and barley has a higher content of the enzymes essen-
tial for breaking down starch into sugar. Barley in its native
form contains 65 percent starch, and this must first be bro-
ken down to simple sugars for the yeasts to metabolize into
alcohol.
The multi‐step process of preparing the barley for brewing
is called malting. First, the barley is soaked in water until it
reaches a moisture content of 45 percent; then it is allowed to
germinate (begin to sprout). At that point, starch content is
at its maximum and starch‐digesting enzyme activity is at its
peak. Sprouting is halted by gently heating the barley (kiln-
ing) to stop further growth (the sprout must not be allowed
to use up the starch, the source of sugars for fermentation).
At the same time, the enzymes must not be killed, so tem-
perature control and exposure time are critical. When kilning
is complete, the malted barley is ground, or milled, to crack
open the grains. Milling the grains makes it easier for them to
absorb the water with which they are mixed in the mashing
process, during which the starches are broken down to sug-
ars, primarily maltose, a disaccharide consisting of two glu-
cose subunits. Maltose is used by the yeast for fermentation.
The final ingredient required for brewing beer is, of course,
the yeast. The yeast used for brewing is the same species used
for baking, Saccharomyces cerevisiae, but they are different
strains, cultivated to possess specific abilities: baking yeast
strains are more active, to leaven dough in the shortest
amount of time possible; brewing yeast strains act more
slowly but tend to produce fewer off flavors and tolerate
higher alcohol concentrations (with some strains, up to
22 percent). Many pure strains of brewing yeast have been
developed to provide the sensory characteristics of the many
styles of beer commonly produced: ales, lagers, stouts,
pilsners, and so on.
The Science of Beer‐Making
Beer is consumed in vast amounts all over the globe. In the
United States alone, over 200 million barrels (6,200,000,000
gallons) are consumed annually. Today beer making is largely
automated, like all areas of food manufacturing. But even
though many breweries use sophisticated brewing equip-
ment, the process remains essentially the same as it has for
centuries. Advances in understanding the science behind the
process have led to huge improvements in quality and
consistency.
After the barley is malted, mashing is the next step in the
beer‐making process. This process converts the starches
released during the malting stage into sugars that can be fer-
mented. The malted barley is mixed with hot water in a large
vessel known as a mash tun to create a cereal mash. During
the mash, naturally occurring enzymes present in the malt
convert the starches in the grain to simple sugars (saccharifi-
cation). The wort (pronounced “wert”) is the result of the
mashing process; it is a sugar‐rich liquid that is strained
through the bottom of the mash tun in a process known as
lautering.
The wort is moved into a large tank, known as a copper
or kettle, where it is boiled with hops and sometimes other
ingredients, such as herbs or spices. Boiling deactivates
enzymes, precipitates proteins, solubilizes hop resins, and
concentrates and sterilizes the wort. At the end of the boil,
the hopped wort undergoes clarification in a vessel called a
whirlpool, where the more solid particles in the wort are
removed.
After the whirlpool, the wort is quickly cooled (typically
using high‐efficiency heat exchangers) to a level where yeast
can be added safely (yeast is killed at temperatures higher
than 120°F [50°C]). Once the wort has cooled sufficiently, it
goes into a fermentation tank. At this point the yeast is added,
or pitched, into the fermentation tank. Now fermentation
begins, and the sugars turn into alcohol, carbon dioxide, and
other minor components. The fermentation process takes
from 2 to 6 weeks, depending on the type of beer being made.
Because fermentation produces a substantial amount of
heat, the tanks must be cooled constantly to maintain the
proper temperature.
The fermentation vessel (or fermenter) is sealed off from
the air except for a long, narrow vent pipe that allows carbon
dioxide to escape. Due to the constant flow of carbon dioxide
through the pipe, outside air is prevented from entering the
tank, reducing the threat of contamination by stray yeasts or
other contaminants. While fermentation is still actively
occurring, and when the specific gravity has reached a prede-
termined level (usually between 1.020 and 1.025, which is
equivalent to 2.25 to 2.875 percent alcohol), the carbon diox-
ide vent tube is capped. Now the vessel is sealed, so as fer-
mentation continues, pressure builds as carbon dioxide
continues to be produced. This is how beer gets most of its
carbonation; the rest is added later. (In the case of most keg
beer, the beverage is additionally carbonated when it is con-
nected to the tap in the bar.) From this point on, the beer
remains under pressure (except for a short time during
bottling).
When the fermentation is finished, the beer is cooled to
about 32°F (0°C). This helps the remaining yeast settle to the
bottom of the fermenter, along with other undesirable pro-
teins that precipitate out of solution at this lower tempera-
ture. The bottom of the fermenter is cone shaped; this makes
it easy to capture and remove the yeast, which may be saved
and used in the next batch of beer. The yeast can be reused a
number of times before it must be replaced. This is when it
has mutated to the point where it produces a different taste—
remember, commercial brewing is all about consistency.
Cataloging the number of times the same batch of yeast is
used for fermentation allows the brewmaster to calculate
when the yeast must be replaced. Sensory evaluation also
ensures the yeast is not producing off flavors.
Once the fermentation process is complete, the beer is
slowly pumped from the fermenter and filtered to remove any
remaining solids. From the filter, the beer goes into a bright
c11.indd 205 1/13/2016 7:54:54 AM
206 • CULINOLOGY®
beer tank, its last stop before bottling or kegging. Here, the
level of carbon dioxide is adjusted by introducing a little extra
gas into the beer through a porous carbonating stone.
The two primary types of beer are the heavier and darker
ales (including stout and porter) and the lighter lagers
(including pilsner, the most popular beer consumed in the
United States). There are two major differences in the brew-
ing process. First, ales are fermented at higher temperatures:
65 to 75°F (18 to 24°C), and lagers are fermented much colder
at 46 to 55°F (8 to 13°C). The second distinction is the type of
yeast used in the fermentation process. Ales generally use
top‐fermenting yeast. This means the yeast floats on the sur-
face for the first few days and then settles on the bottom.
Lagers use bottom‐fermenting yeast, which does not float to
the surface before settling.
A third type of beer is far less common than ales or lagers:
lambics. True lambics are brewed in Belgium only, and typi-
cally are blends of two or more beers, sometimes with the
addition of sugar or syrups as they tend to be quite tart. In
addition, fruit lambics are very popular, with cherry, rasp-
berry, peach, black currant, grape and strawberry versions
commonly available. Unlike ales and lagers, which use pure
cultures of yeast that are specially cultivated for their fermen-
tation, lambics are fermented by wild yeasts.
Making Wine
Winemaking, in its simplest form, involves crushing grapes,
collecting the juice in a vat, and allowing it to ferment. As
yeasts responsible for fermenting the sugars in the grapes are
normally present on the skin of the fruit, fermentation can
begin as soon as the yeasts come into contact with the juice. Of
course, it is not that simple. Selecting the correct varieties of
grapes for their particular flavor characteristics is truly an art,
as is selecting the correct pure strain of yeast. Understanding
the Brix (sugar content) and pH (acidity) of the juice and how
it affects the fermentation of the beverage is also essential, as
is maintaining proper temperature and sanitation.
Because of the large number of types of wine that are pro-
duced, we are restricting our discussion to grape wine. The
species of grapes used in most wines is Vitis vinifera, known
to have been domesticated before 4000 bc.
Wine is made today much the same way it was centuries
ago. Still wines (non‐effervescent) come in primarily three
colors: red, white, and rosé. If fermentation is stopped before
the sugars are all metabolized by the yeast, the finished prod-
uct is sweet wine. If fermentation is allowed to go to comple-
tion and all the sugars are metabolized, the wine is dry. The
winemaker takes deliberate steps throughout the fermenta-
tion process to control the final product.
The grapes from which the wine is to be made are first
stemmed and then crushed in order to release the juice. The
combination of the skin, juice, and seeds is called the must.
Grapes may be crushed by various means, from stomping on
them with bare feet to pressing with sophisticated electric
machinery.
If the desired product is a white wine, the free juice is
transferred to a fermentation tank, one that is typically made
of stainless steel, which is much easier to clean and sanitize
and much longer lasting than wooden barrels. In addition,
unlike wood, no flavors will leach out to potentially contami-
nate the wine. The peels and stems are then removed and
pressed again. The juice of the second press can be added to
the original juice or used to make a lower‐grade wine. It is
important to note that the color of the grape does not neces-
sarily dictate the color of the wine. The juice of both red and
white grapes has relatively little pigment. The color of red
wine comes from the red pigment in the grape skin, not the
juice. If red wine is the desired product, the skins of the grape
must go into the fermentation tank along with the juice. In
fact, some well‐known white wines are made from red‐ or
black‐skinned grapes. Champagne, for instance, uses Pinot
Noir (red‐skinned) grape juice, and the popular white wine
Pinot Grigio is also made from a red‐skinned grape. It is criti-
cal with white wines, however, that the skins be removed
from the juice as soon as possible after crushing so as not to
tint the wine pink.
Once the juice is in the fermentation tank, preferred pure
strains of yeast are usually added but are not absolutely
required, because the skin of the grapes has adequate yeasts
on it already. However, as the wild yeasts that grow on the
grapes vary by vineyard and especially by country, the quality
of the finished wine also varies. Therefore, the addition of a
specially cultivated pure yeast strain gives much greater
measure of control and consistency to the end product. Most
wine yeasts are strains of Saccharomyces cerevisiae, the same
family of yeasts from which both baker’s and brewer’s yeast
originate.
Sulfur dioxide is normally introduced into the juice at this
time to kill any bacteria that may spoil the taste of the final
product. It also acts as an antioxidant, and in red wine it can
help to stabilize color. Sulfite sensitivity is an asthma‐like
reaction caused by exposure to sulfites; the FDA estimates it
affects 1 percent of the population. Because of this, the use of
sulfur dioxide as a preservative in any food or beverage
FIGURE 11.8 Tuscan vineyard.
Source: Charles Hayes.
c11.indd 206 1/13/2016 7:55:04 AM
CHAPTER 11 • Fermentation • 207
(including wine) must be reported on the label if it is present
at greater than 10 ppm (parts per million) in the finished
product to warn individuals who may have breathing difficul-
ties when exposed to it.
Fermentation is allowed to continue for 8 to 10 days, after
which the initial wine is drawn off of the skins, if they are still
present. Any juice pressed from these skins after their removal
is considered to be of lower quality and may be used in bulk
wines or for vinegar production. Following the initial fermen-
tation, the liquid is allowed to ferment for an additional 20 to
30 days. During this time, the dead yeast cells and other par-
ticulate matter settle to the bottom of the tank. When this
process is complete, the wine is separated from the sediment
and transferred to an aging tank. As the aging process contin-
ues, more sedimentation occurs, and the wine is often trans-
ferred across a series of tanks during aging. This process is
known as racking.
After the wine is racked, it may be bottled or transferred to
wooden barrels for additional aging, depending on the wine-
maker and the type of wine being made. Wine barrels, espe-
cially made of oak, have long been used to age wine. Aging in
oak typically imparts desirable vanilla, butter, spice, and
“woody notes” to wine, as well as color and tannins, which
affect mouthfeel and perceived texture. Barrels may be made
of French or American oak, and are typically “toasted” before
use. Toasting involves charring the inside of the barrel with
an open flame, and barrels are available in light, medium, and
heavy toasting, depending on the type and style of wine the
winemaker is after. The most common barrels hold 59 U.S.
gallons (220 L). New barrels impart more flavors than previ-
ously used barrels because over time many of the oak proper-
ties leach out—to the point where, after three to five years,
little or no oak flavors may be imparted to the wine. Due to
the expense of barrels, several techniques for reusing them
have been devised in an attempt to save money. One is to
shave the inside of used barrels and insert new, thin inner
staves.
The porous nature of an oak barrel allows some evapora-
tion and oxygenation to occur during aging. Oxygen enters a
barrel when water or alcohol is lost due to evaporation, a por-
tion known as the angels’ share. Most wines are topped up
from other barrels to prevent significant oxidation. In a year,
the typical 59‐gallon barrel can lose around 5 to 6 gallons of
wine through evaporation. This evaporation (of mostly alco-
hol and water) allows the wine to concentrate its flavor and
aroma compounds.
The length of time a wine spends in the barrel depends on
the grape varietal and the style of wine the winemaker wishes
to make. The majority of oak‐derived flavors are imparted in
the first few months that the wine is in contact with oak, but
longer exposure can affect the wine through the light aera-
tion the barrel allows, which helps develop intrinsic flavors
coming from the wine itself. Many wineries now use oak
wood chips for aging wine more quickly. Oak chips can be
added during fermentation or during aging. In the latter case,
they are generally held in fabric sacks that are placed into the
aging wine. Oak chips have the benefit of imparting intense
flavoring in a matter of weeks, while traditional oak barrels
need a year or more to convey similar intensity. They are also
much cheaper than barrels.
The length of time a wine ages depends on the judgment
of the winemaker. For white wines, it is usually 12 to 18
months, but red wines can age for five years or longer. During
aging, the wine is sampled and assessed regularly by the
winemaker. It may be bottled after aging is complete or used
as part of a blend. While today most wines are made to be
consumed immediately after bottling, some white wines ben-
efit by additional aging under controlled temperature and
humidity storage for up to five years, after which they deterio-
rate. Certain red wines, on the other hand, continue to
improve for 30 years or more.
Champagne and Other
Sparkling Wines
Thus far discussion has covered still wines, which are fer-
mented in open tanks or vented closed tanks to avoid carbon
dioxide buildup and subsequent carbonation. But cham-
pagnes and sparkling wines are produced using a different
method, known as the champagne process. To develop car-
bonation, extra sugar is added to still wine in the bottle while
the yeast is actively fermenting. The bottle is then tightly
capped. As this secondary fermentation continues, carbon
dioxide builds up in the bottle and carbonates the wine. This
is somewhat tricky because if too much fermentation occurs,
the tightly sealed bottle can explode. This is why champagne
bottles are so much thicker and heavier than bottles used for
still wines. The secondary fermentation takes a minimum of
one year but can continue for three years or longer.
Riddling racks are critical to the process of making
Champagne. After the sparkling wine has completed its sec-
ondary fermentation, it is ready to be finished. Remuage or
“riddling” is the process that collects the yeast and sediment
and concentrates it near the mouth of the bottle. The riddling
rack is made up of two rectangular boards hinged at the top.
Each side consists of rows of evenly spaced holes, totaling six
across and ten rows down, each able to hold the neck of a
champagne bottle. This means each riddling rack can hold
120 bottles. The “riddler” (a specialist experienced in the art of
riddling) puts the neck of a bottle of Champagne in each of the
holes, inclined at a 45‐degree angle. A mark placed on the bot-
tom of each bottle acts as a reference marker, with all marks
on all bottles in the rack pointing in the same direction.
Over the next few weeks on a daily basis, the riddler
rotates every bottle a few degrees. At the same time he raises
the bottle’s bottom slightly, lowering the neck a centimeter or
two each week. After a few weeks bottles that started at a
45‐degree angle are now slanted to a 60‐degree angle and are
neck‐down in their holes. After riddling is complete, the
Champagne bottles are placed upside down with their necks
submerged in a sub‐zero solution for several minutes, form-
ing an ice plug made of the spent yeast and sediment in the
necks. They then quickly undergo disgorgement, which
means the crown caps are removed from the bottles, resulting
c11.indd 207 1/13/2016 7:55:04 AM
208 • CULINOLOGY®
in the pressure within the bottles shooting out (disgorging)
the ice plug (with the frozen sediment trapped within it).
The bottle is then quickly topped up with a dosage of
champagne or sugar syrup, depending on the level of sweet-
ness desired, and corked. A wire cage is usually wrapped
tightly around the cork, securely binding it to the top of the
bottle to ensure the cork stays put.
Like still wines, sparkling wines come in different varieties.
Blancs de blanc (white from white) are made exclusively of
white wine grapes; blancs de noir (white from black) are made
from red wine grapes. True French Champagne is made from
a combination of both. There are also pink or rosé cham-
pagnes. By law, the term Champagne can be used only for
wines made in the Champagne region of France. Many other
very good sparkling wines are made by the same process in
other parts of France—in fact, all over Europe and in the
United States, but none of them can legally be called
Champagne. Less expensive sparklers are also widely availa-
ble, typically mass‐produced in large vats (the Charmat or
bulk process) rather than in individual bottles. Champagne is
available with varying levels of residual sugar. The most com-
mon label descriptions are somewhat confusing: Brut wines
are drier (have less sugar), while extra‐dry wines are sweeter.
Wine Fermentation
Fermentation is at the heart of winemaking. The goal is to
convert sugar into ethanol in a way that produces a mini-
mum of undesirable by‐products while preserving the maxi-
mum natural aroma and flavor of the fruit.
Theoretically, 180 g sugar produces 92 g ethanol, which is
51 percent of the sugar weight. About 5 percent of the sugar is
consumed to produce by‐products such as glycerol, succinic
acid, lactic acid, 2,3‐butanediol, acetic acid, and other com-
pounds. About 2.5 percent of the sugar is consumed by the
yeast as a carbon source, and about 0.5 percent is left over as
unfermented residual sugars. Consequently, about 8 percent
(14.4 g) of sugar is not converted into ethanol. The remaining
165.6 grams therefore produce 84.5 g ethanol. From these cal-
culations, a winemaker can determine the alcohol potential
of grape juice by knowing how much sugar it contains.
Through extensive research, favorable yeast cultures for
wine production have been identified and grown in pure cul-
ture. Although about 150 yeast species are known to relate to
winemaking, the most important ones are Saccharomyces
cerevisiae and Saccharomyces bayanus. These two species are
the source material for many strains of yeast developed spe-
cifically for every type of wine, grape, and process in commer-
cial use today.
Commercial yeast strains are sold dry in vacuum‐sealed
packs or as liquid cultures. The recommended dose is about
200 g yeast per 1000 L of must to start fermentation. At this
concentration, the added yeast cells are dominant, and very
soon they take over fermentation from any wild yeasts that
are present. The use of one dominant yeast strain is most
beneficial, making the fermentation more consistent, faster,
and tailored to the winemaker’s needs.
This is in contrast to the Old World tradition of relying on
wild yeast found in the vineyard and on the winemaking
equipment. Such fermentation is commonly used in France
and some California wineries. It is believed that by ferment-
ing with an assortment of naturally occurring yeast strains,
the wine is more complex and more “natural.” When using
wild yeast fermentation, a certain measure of control over
the process is lost. One of the most abundant strains found in
the so‐called natural yeast technique belongs to another spe-
cies, Kloeckera apiculata, which is more active in low alcohol
concentrations (4 to 5 percent alcohol). This can cause
incomplete fermentation, as the yeasts die before the wine
reaches typical alcohol levels of 10 to 12 percent. This results
in a very sweet low‐alcohol wine and is why most winemak-
ers opt for using pure strains of well‐researched and docu-
mented yeast cultivars.
Dairy Fermentations
Milk has been called nature’s perfect food. Milk contains vita-
mins, fats, minerals, carbohydrates, and proteins all in one.
This makes it an excellent source of food for both humans and
microorganisms, both pathogenic and nonpathogenic. Louis
Pasteur was the first to define the process that bears his name
(pasteurization) for heating milk to kill pathogens.
If milk is allowed to sit at room temperature, naturally
occurring lactic acid bacteria start to ferment the primary
carbohydrate, the disaccharide lactose, into lactic acid. This
reduces the pH, causing the milk protein, casein, to coagulate
or curdle and form two phases: curds and whey. Curds are
semisolid lumps consisting primarily of casein and milkfat.
Whey is watery liquid composed of whey protein, lactose,
and other water‐soluble materials. This separation is the first
step in producing many fermented dairy products.
The Streptococcus bacteria Streptococcus lactis and
Streptococcus cremoris are gram‐positive spherical bacteria
capable of fermenting lactose to lactic acid. These two species
are used as starter cultures in conjunction with Leuconostoc
cremoris to make cultured buttermilk, produced by inoculat-
ing pasteurized skim milk and allowing the fermentation to
proceed until the desired acidity, flavor, and aroma character-
istics are reached. The bacteria convert lactose to lactic acid,
acetic acid, ethyl alcohol, carbon dioxide, and two additional
compounds, acetylmethylcarbinol, and diacetyl, all of which
give buttermilk its characteristic flavor and aroma.
Butter is produced by churning (agitating) cream until the
butterfat separates out to form solid butter and liquid but-
termilk (which differs from cultured buttermilk). It can also
be made by first souring the cream to develop the more
intense taste and aroma of cultured butter. This is done com-
mercially by allowing the cream to ferment (using lactic
starter cultures) for approximately 12 hours under controlled
temperature prior to churning. After churning, the butter
may be salted or not, packaged, and refrigerated to allow the
butterfat to crystallize and the butter to harden.
The many other well‐known fermented dairy products
include sour cream, kefir, yogurt, and of course all of the
c11.indd 208 1/13/2016 7:55:04 AM
CHAPTER 11 • Fermentation • 209
hundreds of types of natural cheeses that are distinguished
by their end‐product attributes, by the type of milk used for
the fermentation, and by the microbial agents involved.
Cheese‐making is as much a mixture of art and science as
is fermentation itself. One reason is the natural variations in
milk produced by different breeds of dairy cattle. Milkfat var-
ies extensively between breeds of dairy cows, with Jersey and
Guernsey having the highest amount of milkfat and Holstein
the lowest. The type of feed given to cows can also affect the
overall composition of the milk, with grain‐fed livestock pro-
ducing higher milkfat. After birthing, the cow produces milk
for her young calf. The milk composition changes based on
how recently she gave birth and how long since she was last
milked, the age and overall health of the cow, seasonal differ-
ences (percentage of milkfat is higher in the cooler part of the
year, especially winter, and lower in the summer, due to the
quality and quantity of the pasture grass available to use as
feedstock), and nutrition and feeding management, which
affect the volume yield in addition to composition. Last but
certainly not least is the effect of pasteurization on the qual-
ity of the milk.16
There are approximately 18 types of natural cheeses, cat-
egorized by the different processes used to make them. These
include brick, Camembert, cheddar, cottage, cream, Edam,
Gouda, hand, Limburger, Neufchâtel, Parmesan, provolone,
Romano, Roquefort, sapsago, Swiss, Trappist, and whey
cheeses (such as ricotta).
Unlike most cheeses, which are prepared from curds,
ricotta cheese is a fresh, soft, creamy, and lightly flavored
cheese produced from the lactic fermentation of whey, which
is the major by‐product in all cheese manufacture. (After the
curds are separated from the whey, it is the whey itself that
becomes the source material for ricotta.) For every 5 gallons
of milk made into cheese, there is enough whey to produce
approximately 2 pounds of ricotta. As the whey contains lac-
tose, lactic acid bacteria are added to convert it to lactic acid,
FIGURE 11.9 Dairy fermentation product matrix.
Fermented Product Dairy Substrate Principal Microbe Process
Bulgarian buttermilk Pasteurized milk Lactobacillus bulgaricus Incubate inoculated milk @
98.6°F; follow same process
as cultured buttermilk.
Yogurt Concentrated skim milk Lactobacillus bulgaricus &
Streptococcus thermophilus
Incubate inoculated substrate
for 3–4 hours at 105°F, then
cool to 40°F.
Butter Churned milk to collect
cream solids
Streptococcus cremoris &
Leuconostoc citrovorum
Incubate inoculated substrate
@ 86°F until acid spec is
reached.
Cultured sour cream Cream Streptococcus cremoris &
Leuconostoc cremoris
Incubate inoculated substrate
@ 86°F until acid spec is
reached.
Acidophilus milk Pasteurized milk Lactobacillus acidophilus Incubate inoculated milk
@ 98.6°F and ferment
until acidity of 0.6–0.7% is
developed.
Kefir; native to Eastern
Europe and Western Asia;
means pleasant taste
Milk from cow, goat,
or sheep
Streptococcus lactis,
Lactobacillus bulgaricus, &
lactose-fermenting yeast;
uncontrolled fermentation
Milk is allowed to ferment in
bags made of goat skin, and
fresh milk is continually added
after the fermentation is
active. Grains are formed
of bacteria, yeast, and
protein. Contains 1% acid
and 1% alcohol and carbon
dioxide gas, which gives it a
fizzy taste.
Kumiss; native to Western
Asia; sometimes called
milk‐wine or milk beer
Horse milk Streptococcus lactis,
Lactobacillus casei,
& lactose-fermenting yeast
Mare’s milk is allowed to
ferment in bags made of
goat skin. Contains 0.7–1.3%
acid and >1% alcohol in
conjunction with carbon
dioxide gas.
Source: James Adams.
c11.indd 209 1/13/2016 7:55:04 AM
210 • CULINOLOGY®
causing the pH of the solution to drop. This acidic solution is
then heated to near boiling, which denatures the whey pro-
tein, causing it to precipitate into small curds. The ricotta is
then strained and packed into containers and refrigerated.
Ricotta can also be frozen for extended periods to extend its
shelf life.17
Cheeses are classified as hard, soft, or semi‐hard depend-
ing on their moisture content and on whether they are rip-
ened or unripened. Unripened cheeses (such as cottage
cheese, mascarpone, and goat cheese) are fermented in one
step and generally consumed fresh. After the initial fermenta-
tion step, ripened cheeses are aged for various amounts of
time, during which additional fermentation and flavor devel-
opment takes place.
As an example of the cheese‐making process, the focus here
is on production of the most popular natural cheese in the
United States, Canada, and some parts of Europe: cheddar.
Cheddar curd is formed as a result of controlled fermenta-
tion, where the type, temperature, and amount of milk, bacte-
rial culture used, concentration of rennin (an enzyme that
coagulates soluble milk casein into insoluble protein to pro-
duce the curds), humidity, and amount of acid produced are
all closely monitored. Although the cheese can be made from
either raw or pasteurized milk, if raw milk is used the cheese
must ripen for 60 days or more by U.S. law as a safeguard
against the development of pathogenic organisms such as
E. coli, salmonella, and listeria.18 Most cheddar cheese made
today uses pasteurized milk.
To form the separate curd and whey fractions, pasteurized
milk is pumped into a stainless steel, open‐topped trough‐like
tank (referred to as a cheddaring vat) and heated to approxi-
mately 86°F (30°C). A starter culture of the lactic acid bacte-
rium Streptococcus lactis is added to the tank, along with
yellow‐ or orange‐pigmented natural coloring agents (an natto,
turmeric, or carotenoids, like beta carotene) to produce the
familiar orange cheddar color. (White cheddar is made with-
out these natural colors.) An agitator completely mixes the
color and the bacterial inoculum evenly in the milk. Within 30
to 45 minutes, lactic acid is produced at a low concentration
(acidity rises 0.01 to 0.02 percent) and rennin in a dilute solu-
tion is added (to coagulate the protein and form the curd).
After the enzyme is added, the agitation is turned off. In the
cheese business, this is referred to as setting the milk.
After about 30 minutes, a curd similar to soft custard starts
to form in the tank, and with more acid being produced as fer-
mentation progresses, the curd reaches the desired firmness.
The next step is to cut the curd with a curd knife, made of wires
strung across a frame in a grid pattern similar to a square ten-
nis racket. This takes some skill. By drawing the curd knife the
length of the tank and then with a back‐and‐forth movement
the width of the tank, the cheese curd is cut into small cubes,
promoting the separation of the whey from the curd. After cut-
ting the cubes, gentle agitation is turned back on and heat is
applied to the tank to bring it back up to 86°F (30°C) over
approximately 45 minutes. This causes the cubes to shrink,
which helps squeeze out the whey, thus firming up the curds,
the whole mass taking on the appearance of cottage cheese.
After heating, the agitation is stopped and the curds are
allowed to settle in the tank. They are trenched along the sides
of the tank using a trowel, which helps the whey to drain. The
whey may then be drained off and captured for use in making
other dairy products, such as ricotta cheese.
The curds are allowed to knit together for about 15 min-
utes, forming a solid mass with a rubbery consistency. They
are then cut into large blocks, approximately 40 pounds each,
and stacked on top of each other to allow additional whey to
drain. The blocks are turned every 15 minutes; this handling
and movement squeezes more whey from the curds. The
process is referred to as cheddaring and is unique to the pro-
duction of cheddar cheese.19
The cheddaring step is completed in about two hours,
when chemical analysis of the expressed whey is between 0.5
and 0.7 percent acidity, indicating the curd blocks are ready
for milling. Milling is the process of shredding the blocks into
irregular pieces approximately 0.5 inch in size, which is nec-
essary to facilitate uniform salting of the cheese. The salt—
between 1 and 3 percent by weight—is mixed throughout the
milled curd. It is added to draw out any remaining whey and
reduce moisture content even further, and to add flavor to
the cheese. After salting, the milled curds are placed in hoops
lined with cheesecloth. These are placed in a press, where
they are pressed overnight to reach a specified moisture, usu-
ally 30 to 40 percent.
After the pressing, the hoops are removed and the cheeses
placed on racks in a temperature‐controlled room to chill for
FIGURE 11.10 Classification of cheeses.
SOFT
Unripened:
Low-fat—cottage, pot, baker’s
High-fat—cream, Neufchâtel (U.S. made)
Ripened: Bel Paese, Brie, Camembert, cooked, hand,
Neufchâtel (French made)
SEMISOFT
Ripened: principally by bacteria: brick, Muenster
Ripened: by bacteria and surface microorganisms:
Limburger, Port du Salut, Trappist
Ripened: principally by blue mold in interior: Roquefort,
Gorgonzola, blue, Stilton, Wensleydale
HARD
Ripened: by bacteria, without eyes: cheddar, granular,
caciocavallo
Ripened: by bacteria with eyes: Swiss, Emmentaler,
Gruyère
VERY HARD
Ripened: by bacteria: aged Asiago, Parmesan, Romano,
sapsago, Spalen
PROCESS CHEESES
Pasteurized, cold‐pack, related products
WHEY CHEESES
Mysost, Primost, ricotta
Source: G. P. Saunders, 1953.
c11.indd 210 1/13/2016 7:55:04 AM
CHAPTER 11 • Fermentation • 211
the next three to four days. The cheese is now ready for the
ripening process, wherein the bacteria and the enzymes con-
tinue to modify the texture, flavor, and color of the cheese by
continuing to ferment leftover lactose into acids and aroma
compounds. (In addition to lactose, which is mostly metabo-
lized early in the process, there is also some breakdown of
fats and proteins, which leads to the development of many of
the flavor and aroma compounds later in the aging process.)
The organoleptic changes (taste, texture, appearance, and
aroma) are quite considerable during the aging or ripening
period, which lasts for a minimum of 60 days (mild cheddars)
and can continue for a year or longer (extra‐sharp varieties).
Bread
It is believed that yeast‐leavened bread, often called the “staff
of life,” has been around since the Neolithic Age (10,000 bc).20
Initially, the yeasts involved were wild species that lived on the
surface of cereal grains or were present in the air. But over the
centuries, civilizations learned to use the dregs from making
beer or the fermenting grape juice from winemaking to start
the fermentation process that leavens dough. It also became
commonplace to use a bit of leftover dough from the previous
day’s bread‐making to use as a type of sourdough starter.
To make good bread, the dough must be extensible
(stretchable when pulled) so it can expand while rising, elas-
tic (having the strength and flexibility to hold the gases pro-
duced by the yeasts during fermentation), and stable enough
to hold its shape and cell structure. Gluten gives dough all of
these properties. That said, gluten as such does not exist in
wheat flour. Instead, two proteins present in the flour, glu-
tenin and gliadin, are the precursors that form gluten when
flour is mixed with water and then kneaded. Other grains
may be used to make bread, but most lack sufficient gluten‐
building ability to produce a well‐risen, light, and airy loaf.
Therefore, they must be blended with a percentage of wheat
flour (or mixed with pure gluten) to make breads that will rise
and have a delicate, porous structure when baked.
The other important event that occurs when the flour is
wetted is that starch‐digesting enzymes are activated. These
begin to break down the starch into sugars, which in turn
provide food for the yeast, which ferments the sugars into the
carbon dioxide that allows the dough to rise.
Yeast’s role in baking is to provide leavening through the
production of carbon dioxide, which provides texture
improvement through loaf aeration and develops internal
crumb structure (which sets on baking). Yeast also provides
flavor (taste and aroma) due to the formation of dozens of
flavor compounds that are the result of fermentation. Baker’s
yeast is yet another strain of Saccharomyces cerevisiae, the
same yeast responsible for the majority of brewing and wine-
making. It is available in several forms:
• Cream yeast is a suspension of yeast cells in liquid
(essentially a yeast slurry) produced in large industrial
fermenters and sold in bulk containers. Its primary use
is in industrial bakeries with special high‐volume dis-
pensing and mixing equipment.
• Fresh (compressed or “cake”) yeast has 70 percent mois-
ture, requires refrigeration, has a short shelf life
(2 weeks), and is “instantly active” (it’s alive!). Typically
available for commercial use in one-pound blocks looking
very similar to blocks of butter. Occasionally available in
supermarkets in one‐ounce foil‐ or paper‐wrapped cubes.
• Active dry, the original spray‐dried yeast, has a long shelf
life (months to years), requires proofing in warm water
with a bit of sugar prior to usage, and contains 25 per-
cent or more dead cells.
• Instant dry is a more modern version of Active dry which
undergoes a milder drying process that yields fewer
dead cells, and therefore can be used at a 25 percent
lower use rate compared with Active dry. It has a finer
texture, longer shelf life, and does not require advance
proofing—it can be added directly to the dry dough mix.
In addition to the pure culture Saccharomyces yeasts, sour-
dough starter can be used to leaven bread. Sourdough starter
is a combination of wild yeast and lactobacillus bacteria that
provides leavening along with a typical sour flavor in the
bread. Because it is not a pure yeast culture, it takes 2 to 3
times longer to generate the required carbon dioxide for loaves
to rise, but that extra time allows for greater flavor develop-
ment. If cared for properly, starters can last for decades.
Bread‐making involves the following basic steps.
1. Mixing the ingredients to evenly distribute them and form
the dough. Water, flour, yeast, and usually salt are the most
basic, but other ingredients, including dough conditioners,
sweeteners, eggs, oils, extra gluten, whole grains and seeds,
herbs and spices, raisins, olives, and nuts, may be added to
produce specific types of bread or rolls. Mixing also
hydrates the flour to start the development of gluten and
activates the starch‐digesting enzymes.
2. Kneading or working the dough to enhance gluten forma-
tion and provide heat through mechanical energy, thus
increasing enzyme activity and yeast metabolic activity.
3. First rise, in which the dough is allowed to rest, covered, in
a warm, draft‐free area. The dough relaxes and expands as
carbon dioxide begins to build up. During this period, each
yeast cell forms a center around which carbon dioxide
bubbles form. Thousands of these tiny bubbles, each sur-
rounded by a thin film of gluten, form cells within the
dough. As these cells continue to fill with gas, the dough
increases in size. Usually the dough ball is allowed to rise
until it doubles in size.
4. Punching down, in which the doubled dough is punched to
release any large pockets of gas that may have formed,
kneaded briefly to evenly distribute gas bubbles, then cut
and shaped to fit the baking pans.
5. Second rise, which allows the panned dough to rise again
to allow finer crumb structure and more intense flavors to
develop. Once it has doubled in size again, it is baked.
6. Baking transforms the raw dough into a light, porous, fla-
vorful, easily digestible loaf of bread. As the oven heat pen-
etrates the dough, trapped gases rapidly expand, causing a
c11.indd 211 1/13/2016 7:55:04 AM
212 • CULINOLOGY®
sudden increase in the size of the loaf. This is called oven-
spring. Whatever alcohol has been produced during fer-
mentation evaporates. As the temperature in the dough
rises, so does the rate of yeast activity until the tempera-
ture reaches 115°F (46°C), at which point the yeasts die.
From about 140°F (60°C) onward, stabilization of the
crumb structure begins. From 165°F (74°C), the gluten
strands surrounding the individual gas cells become rigid,
providing additional structure to the crumb.
Enzymes present in the dough, in particular alpha
amylase, the enzyme that breaks starch into sugars, con-
tinue working until the dough temperature reaches 167°F
(75°C). Because the yeasts all died at 115°F (46°C), the extra
sugars produced by the enzymes between the death of the
yeasts and the denaturation of the enzymes are not metab-
olized and hence provide sweetness to the bread and
enhance the attractive brown color of the crust. When the
internal temperature of the loaf reaches 208°F (98°C), the
bread is considered done. By this time, surface tempera-
ture can be in excess of 392°F (200°C)—essentially, the
temperature of the oven. The loaf is removed from the
oven for cooling. The loaf is also full of saturated steam
that must be given time to evaporate. The loaves must be
cooled to about 95°F (35°C) before being sliced or pack-
aged to prevent damaging the loaf.
Meat Fermentation
The fermentation of meat is quite similar, microbiologically
speaking, to other processes that have been discussed, fol-
lowing the same course as other lactic acid fermentations.
There is typically not enough carbohydrate in meat to sup-
port sufficient fermentation activity, so they must be aug-
mented by the addition of other sugars, usually dextrose. The
dextrose is fermented by lactic acid bacteria; the acid reduces
the pH, helping preserve the meat and extend its shelf life.
Examples of fermented dried sausages are pepperoni,
Genoa salami, chorizo, Italian salami, hard salami, and cured
meat sticks ( jerky is not included in this list because it must
be cooked in high humidity—no less than 90 percent—to
achieve microbial lethality before drying).21 Semi‐dried fer-
mented meats include summer sausage, Lebanon bologna,
and Thuringer sausage.
Dry and semi‐dry sausages make up the largest group of
all dried meats. Although the USDA does not formally define
semi‐dry or dry sausage, it does regulate the moisture‐to‐pro-
tein ratio (MPR) as follows:
• Shelf‐stable semi‐dry sausages must have a MPR of 3.1:1
or less and a pH of 5.0 or less. They must undergo a mois-
ture loss of up to 15 percent of the total and have a final
water activity (a
w
) range of 0.90 to 0.94. These sausages
are generally cooked or smoked prior to sale and
consumption.
• Shelf‐stable dry sausages must have a MPR of 1.6 to 2.3:1
and a pH of 4.7 to 5.0. They must undergo a moisture loss
of up to 25 percent of the total and have a final a
w
range
of 0.85 to 0.91. These products may be produced without
any cooking step if their water activity is low enough.
Those that are not cooked must have a pH of 5.0 or less
and a
w
of 0.85 or less.22
In addition, in light of outbreaks of E. coli O157:H7 linked
to dry fermented shelf‐stable sausage products, all proce-
dures for either dry or semi-dry sausages must be tested
microbiologically to confirm that a 5‐log reduction of E. coli
O157:H7 has been achieved.23
Fermentable carbohydrate substrates (sugars) normally
make up only about 1 percent of fresh meat. This is not
enough to propagate a strong lactic fermentation, and dex-
trose is usually added to the meat emulsion (consisting of
minced meat, fat, spices, flavorings, curing salts, and culture)
to start the fermentation. The final fermentation pH of the
sausage depends on the initial pH of the raw meat matrix and
the amount of sugar added to allow sufficient formation of
lactic acid by the lactic bacteria.
An example of a fermented dry sausage formulation and
process is the manufacture of U.S.–style pepperoni. According
to the USDA’s Farm Service Agency, pepperoni is the most
popular pizza topping; approximately 36 percent of all pizza
orders are for pepperoni pizza, and over a quarter of a billion
pounds of pepperoni are consumed on pizzas per year in the
United States. Five large chains lead the consumption and
sale of pizza in the United States, as posted on November 1,
2015, by Pizza Today magazine: Pizza Hut, headquartered in
Plano, TX, and a division of YUM Brands, is number one, with
$13.4 billion in annual sales; Domino’s, headquartered in Ann
Arbor, MI, is second, with annual sales of $8.9 billion; Little
Caesars, headquartered in Detroit, MI, is third, with $3.4 billion
in annual sales; Papa John’s, headquartered in Louisville, KY,
has reached $3.32 billion in annual sales and is fourth; Papa
Murphy’s International, based out of Vancouver, WA, is now
fifth in annual sales, with $785 million. With expected growth
of the pizza category, it is easy to see how this fermented
sausage became so popular.
Standard pepperoni is formulated to approximately 30
percent fat using more pork than beef, unless it is labeled as
“all‐beef pepperoni.” Typically, the ratio is 60 percent pork and
40 percent beef, although manufacturers can use up to 55
percent beef in regular (non‐all‐beef ) pepperoni. The pork
percentage is determined on a least‐cost formulation basis
and can be up to 100 percent of the meat material, depending
on market conditions. The meat commonly used is cheek
meat and striated skeletal muscle due to its lack of binding
and its high protein content. The lack of binding is important
to prevent the cupping of pepperoni slices when heated on
pizza, and the high protein level is necessary to achieve the
deep red color of the sausage.
Non‐meat ingredients include salt added at about 3 percent,
dextrose added at about 0.8 percent, spices and flavorings
added at about 2 percent, and starter culture of Pediococcus
acidilactici, which is added to the meat mince at approximately
10 million CFU/g (colony‐forming units of bacteria per gram) to
ensure a reproducible lactic fermentation.24
c11.indd 212 1/13/2016 7:55:05 AM
CHAPTER 11 • Fermentation • 213
The ground meat is tempered to approximately 28°F
(–2°C) before mixing. After the meat is mixed with the spices,
starter culture, and other ingredients, the matrix goes
through a final grind and is stuffed into casings. The stuffed
product is transferred to the fermentation room, where the
temperature is approximately 105°F (41°C) and the relative
humidity is controlled at 80 to 90 percent. Fermentation con-
tinues until a pH of 5.3 or lower is reached and residual sugar
in the meat is measured at 0 percent; in most cases, this takes
12 to 15 hours. The pepperoni is then transferred to the dry-
ing room, where it is monitored for moisture loss, cooled to
approximately 55°F (13°C), and held for 12 to 14 days or until
the moisture‐protein ratio reaches 1:6 or less. During this
cooling and drying time, the relative humidity is closely
maintained between 68 and 72 percent.
Semi‐dry sausage is manufactured in a similar but not
identical manner. Semi‐dry does not lose as much moisture,
and the drying process occurs during the fermentation. After
this process is completed, the sausage is cooked or hot‐
smoked to a minimum internal temperature of 140°F (60°C).
Fish Fermentation
Although they are not common in the United States, a wide
variety of fermented fish products are produced and con-
sumed in Asia and Africa. Some are used as condiments,
some as side dishes, and some as sauces or pastes. The major-
ity of these products are found in Southeast Asia, including
but not limited to Vietnam, Indonesia, Thailand, Malaysia,
and the Philippines.25
More widely known in the United States are the Asian fish
sauces like nuoc mam, a pungent, salty liquid made from fer-
mented anchovies that is used as an ingredient in Vietnamese
cooking. Nam pla is one of the basic ingredients in Thai cook-
ing, again made from fermented anchovies or, sometimes,
squid. The rich, reddish brown sauce is used to marinate
meats and as a condiment, much like Americans use salt and
pepper. It is often mixed with fresh‐cut chili peppers and lime
juice. Other countries have their own varieties made from an
assortment of fish and shellfish species, including patis from
the Philippines and belancan (made from fermented krill or
shrimp) from Malaysia. The majority of bacteria responsible
for fermentation of fish sauces are gram‐positive, halophilic
organisms of the bacillus species that originate within the
bodies of the fish themselves.
In general, fish sauce is made from small fish (either fresh‐
or saltwater species) that would otherwise have little value
for consumption. Larger varieties of fish, such as mackerel
and sardines, also make good fish sauce, but because they are
relatively more expensive due to their value as a food fish,
they are seldom used in the commercial production of fish
sauce. As soon as fishing boats return with their catch, the
fish are rinsed and drained, then mixed with sea salt—2 to 3
parts fish to 1 part salt by weight. They are then placed in
large earthenware jars lined on the bottom with a layer of salt,
and topped with a second layer of salt. A woven bamboo mat
is placed over the fish and weighted with heavy rocks to keep
the fish from floating when the water inside them is drawn
out by the salt and fermentation process.
The jars are covered and left in a sunny location for nine
months to a year. From time to time they are uncovered to air
out and to expose the fish to direct sunlight, which helps
digest the fish and liquefy them. The periodic sunning pro-
duces a fish sauce of superior quality, giving it a fragrant
(some would say nauseating) aroma and a clear, reddish
brown color. When fermentation has finished, the liquid is
removed from the jars, preferably through a spigot on the bot-
tom of the jars so it passes through the layers of fish remains,
or by siphoning. Any sediments are strained out with a clean
cloth. The filtered fish sauce is placed in clean jars and
allowed to air in the sun for a couple of weeks to dissipate the
strong fish odors. It is then ready for bottling.
A similar fish sauce called garum or liquamen was ubiquitous
in ancient Greek and Roman cooking. It was fermented from a
variety of fish (especially the intestines) including tuna, mack-
erel, moray eel, and anchovies. The closest relative of fermented
fish sauce common in Western cuisine today is Worcestershire
sauce, a fermented sauce containing anchovies.
Vegetable
Fermentation
Genghis Khan and his armies are often credited with intro-
ducing fermented vegetables into Europe; however, there is
little doubt the methods for fermenting vegetables existed
long before then. From archeological evidence, it appears
that cucumbers and cabbages were the main vegetables used
in early fermentations.26
Fermented cabbage is the precursor to what is known today
as sauerkraut. Although it has lost some fans, it is still con-
sumed heavily by Americans of German and Eastern European
descent. The classic Alsatian cured meat and potato dish
choucroute uses sauerkraut as its primary ingredient and is
enjoyed throughout Western Europe. And here in the United
States, a hot dog without sauerkraut is unthinkable. Also, there
seems to be a renewed interest in using sauerkraut in nontradi-
tional ways—even as part of dessert. For example, a chocolate
cake made with sauerkraut is moist and has a distinct tangy
taste that complements the flavor of the chocolate.
Lactic acid–fermented vegetables are rich in vitamins and
minerals, nutrients, fiber, and digestion‐enhancing enzymes.
They are also an excellent source of probiotics—that is, the
lactic bacteria primarily responsible for their fermentation.
This method of preserving remains the most nutritious way
to store vegetables out of season. In addition to cabbage and
cucumbers, beets, turnips, radishes, cauliflower, green beans,
and okra are all commonly fermented using salt brines or by
dry salting. The microbe responsible for the fermentation of
these vegetables (including sauerkraut) is Leuconostoc mes-
enteroides, a gram‐positive, facultative halophile (able to tol-
erate relatively high levels of salt).
One of the most famous fermented vegetable mixtures is the
Korean dish kimchi, a mixture of Chinese cabbage, radishes,
c11.indd 213 1/13/2016 7:55:05 AM
214 • CULINOLOGY®
chilies, garlic, ginger, and fish. Kimchi is the national dish of
Korea, where it is eaten every day by most of the populace.
Traditional families eat it with every meal. In November, Korean
fresh vegetable markets are inundated with people trying to get
enough raw vegetables to make a year’s worth of kimchi.
Kimchi fermentation takes place in an earthen jar or large
crock over three to six days at a temperature no higher than
68°F (20°C). The crock is buried in the ground up to the neck
and either covered with straw or fashioned with a plastic head,
similar to the one used in pickle fermentation. When the pre-
ferred sourness level is achieved and no gas bubbles are visible,
nontraditional Korean families remove the crock or jar and
refrigerate the kimchi for up to six months. Traditional Korean
families store the kimchi in the ground throughout the winter
months, removing the contents as needed.
Coffee Fermentation
Most people are unaware that the cup of joe they enjoy as
their morning pick‐me‐up is actually a fermented beverage.
However, it is much different from beer and wine, as the cof-
fee beverage is not a result of fermentation; rather, the beans
are processed by fermentation.
Coffee processing consists of several steps:
1. Coffee beans are picked and separated, and the ripe cher-
ries are sent to the pulping machines.
2. Pulping is used to remove the coffee beans from the ripe
cherries. This is accomplished by applying pressure to
push the coffee cherries against a screen with holes just
large enough for the actual coffee bean to pass through.
(Green cherries are hard and cannot be pulped.)
3. The coffee beans are now ready to be fermented by gram‐
negative bacteria (most often bacteria of the Escherichia
genus), which produces enzymes that remove the slippery
mucilage, a slimy film made up of pectin‐like materials
that covers the bean’s surface. This must be done before
the beans can be dried properly. The fermentation takes
one to two days, depending on the temperature, and is
complete when the bean can be rubbed between the
fingers without sliminess.
4. The beans are transferred to patios, where they are air‐
dried to a moisture content of 11 to 12 percent.
5. A 300 g sample of beans is then roasted and brewed to
determine coffee quality (flavor and aroma being the most
significant quality factors measured).27
Two main species of coffee make up the world’s produc-
tion: Coffea arabica (arabica coffee; 80 percent) and Coffea
canephora (robusta coffee; 20 percent). The robusta is much
more strongly flavored and has higher caffeine content, while
the arabica is more pleasant to the taste, with less bitterness
and lower caffeine. While the USDA forecasts world coffee
production to be down slightly to 127.4 million bags of beans
(1 bag = 100 pounds), it expects U.S. imports to increase by
1.8 percent to 5.8 million bags.28
Note: Although the tea industry refers to tea processing as
fermentation, unlike coffee beans, which undergo true micro-
bial fermentation, tea leaves are not actually fermented.
Instead, they undergo non‐microbial enzymatic oxidation,
which transforms the fresh, grassy aromas and flavors of
fresh‐picked tea leaves into the recognizable flavors and
flowery aromas expected in brewed tea.
Soy Fermentation
There is a great deal of disagreement in the scientific and
popular literature about fermented versus unfermented soy-
foods. In addition to being an excellent source of nutrients
(complete protein, carbohydrate, and fat, as well as vitamins
and minerals, including calcium, folic acid, and iron), soy-
beans also contain large quantities of potentially toxic chem-
icals, including potent enzyme inhibitors that block the
action of trypsin and other enzymes needed to digest protein;
hemaglutenins (blood‐clotting agents); goitrogens, which
affect the thyroid; and phytates (antioxidant compounds that
bind to certain dietary minerals and inhibit their absorption).
These compounds are not completely deactivated during
cooking, and some say they can cause serious gastric distress
as well as deficiencies in amino acid and mineral uptake.
FIGURE 11.11 Recipe for chocolate cake with
sauerkraut.
Chocolate Cake with Sauerkraut
• 1 cup canned or bottled sauerkraut, drained and then
chopped into ¼“ pieces
• 3 whole eggs
• ½ cup butter
• 1½ cups white sugar
• 1 tsp. vanilla
• 2 cups all‐purpose flour
• 1 tsp. baking soda
• ½ tsp. salt
• ½ cup unsweetened cocoa powder
• 1 cup water
• Pre-heat oven to 350°F (325°F if using convection oven).
• Prepare drained sauerkraut and set aside in plastic bowl.
• Beat the eggs and set aside.
• Mix butter, vanilla, and sugar together and slowly beat
the eggs into the mix until a paste‐like consistency is
reached.
• Add the flour, baking soda, salt, and cocoa powder to the
above mix and add water as needed to reach a smooth
paste‐like consistency.
• Add drained, chopped sauerkraut to the mix and stir until
completely uniform.
• Pour the mixture into greased round spring cake pans or
use non‐stick pans if preferred.
• Bake for 30 to 35 minutes and test the cake for doneness
by using a toothpick or clean butter knife. When done,
allow to cool for a minimum of 30 minutes. Cake should
be frosted only after cooling.
• Prepare frosting recipe or drizzle with chocolate ganache.
Source: James Adams.
c11.indd 214 1/13/2016 7:55:05 AM
CHAPTER 11 • Fermentation • 215
Diets high in trypsin inhibitors cause enlargement and severe
complications of the pancreas, including cancer.29 On the
other hand, the counterargument says that when eaten in
moderation, unfermented soy products are not dangerous.
Regardless of which is correct, one thing both sides can agree
on: Fermented soyfoods (soy sauce, miso, natto, tempeh) do
not contain these compounds, and as such are risk‐free and
highly nutritious. (Note: Edamame are a strain of soybeans
grown to be eaten as a green vegetable. While they do contain
measureable amounts of anti‐nutritional factors, these are
easily removed by blanching, unlike the dry‐bean variety of
soybeans.)
Originally prized in China for their soil‐improving abilities
(like all legumes, soybeans fix nitrogen in soil where they are
planted, thus improving its fertility for growing other crops that
lack this ability), once the Chinese discovered how to ferment
them (between 1134 and 246 bc, during the Chou dynasty),
they took full advantage of soy nutrients without negative side
effects. Due to their exceptional nutritional profile and taste,
fermented soyfoods are popular staples throughout Asia and
the rest of the world. Many products consumed around the
world are produced by the fermentation of soybeans.
• Natto is a traditional Japanese food made by the alkaline
fermentation of the amino acids in soybeans by Bacillus
subtilus. Whenever amino acids are fermented, the result-
ing product smells like ammonia. Natto has this strong
characteristic odor, along with a slimy, sticky texture.
However, if one can get past the texture and aroma, natto
offers health benefits not found in other foods, as the
bacillus bacterium secretes blood clot–dissolving
enzymes. Natto has been linked to the prevention of heart
attacks and cancer.30 Additional research must be con-
ducted to verify these links.
• Miso paste is another traditional Japanese food made
from fermented soybeans. It is made by combining
cooked soybeans and koji (a starter made of rice or
barley, or a combination of the two that has been fer-
mented with a mold, Aspergillus oryzae) with salt and
water and fermenting them in wooden kegs for up to
two years, depending on the attributes wanted in the
final product. During the fermentation of koji, the mold
produces enzymes that break down proteins and car-
bohydrates of the soybeans, which are turned into an
umami‐laden paste filled with glutamates as a result.
There are several common varieties of miso: “White”
miso undergoes the shortest fermentation and is the
mildest “yellow” miso is intermediate in strength, and
“red” miso undergoes the longest fermentation and is
strongest in flavor. Beyond the basic three, there are
dozens of variations based on the ingredients being fer-
mented and the fermentation protocol used, but all are
made with koji. Koji is to miso what malt is to beer.
Miso is commonly consumed as the chief flavoring in a
thin, brothy soup, considered a medicinal food by the
Japanese, who drink it daily.
• Tempeh has been a traditional Indonesian food for over
300 years. It is made by soaking hulled soybeans over-
night, then cooking them briefly, allowing them to cool to
80 to 85°F (27 to 29°C), then adding a starter culture of
Rhizopus mold spores (usually R. oligosporus or R. ory-
zae). Following an incubation and fermentation period of
approximately 48 hours at 86°F (30°C) and approximately
FIGURE 11.12 Miso varieties.
Type Red Miso White Miso Barley Miso Soybean Miso
Grain Source White rice, barley, or
soybeans
Rice koji (60%)
and fewer soybeans
Barley grains, soy-
beans, and barley
koji
Only soybeans
Appearance Reddish brown Tan to light brown with
a very smooth texture
Very dark brown color Reddish brown and
chunky texture
Fermentation time 1–3 years 1–2 weeks
(storage at 2 months
refrigerated)
1–3 years Minimum of 1 year
Comments Contains the highest
amount of protein of
all the miso products
Contains the highest
amount of carbohy-
drate of all miso
products
Cheapest of all miso
products
Very salty taste
Made with Aspergillus
hatcho
Considered “the miso
of emperors”
Food
Applications
Stir fries, miso soups
and stews, and to
make marinades for
meat, poultry, and
vegetables
Light-colored soups,
salad dressings, and
marinades for fish
Seasoning rich soups,
stews, beans, sauces,
and spreads
Used to flavor decadent
and hearty soups
Source: Adapted from About Soya.be, 2009.
c11.indd 215 1/13/2016 7:55:05 AM
216 • CULINOLOGY®
70 percent humidity, the tempeh is formed into a com-
pact white cake. In the process of partially metabolizing
the soybeans, the mold eliminates whatever anti‐nutri-
tional factors are present in the undercooked beans,
making them entirely digestible. Tempeh has a nutty,
mushroom‐like flavor when sliced and fried in vegetable
oil until it begins to crisp and turn brown. The fried tem-
peh is added as a high‐protein and high‐fiber source to
soups, salads, and sandwiches, and is used in the making
of spreads.31
• Soy sauce, the best known of the fermented soy prod-
ucts, is one of the essential flavoring ingredients in Asian
foods. It has been used in China for over 2500 years.
In Japan, “shoyu” is the Japanese name for soy sauce, which
is made from a mash of soybeans and wheat, while “tamari” is
a non‐wheat product made by drawing off the liquid content
of soybean miso. The actual translation of tamari is “puddle,”
so called by the way it would pool on top of the miso.
Because genuine tamari is a non‐wheat product, it has a
distinctive aroma as well as thicker texture, deeper color, and
stronger taste than shoyu. It is often used for dipping raw fish
(sashimi), sautéing teriyaki, and other food processing, while
shoyu is used as an all‐purpose cooking soy sauce. There are
only certain areas in Japan where tamari is produced, whereas
shoyu is much more widely available.
Traditional soy sauce is made by a three‐step process:
1. Producing a koji (as in making miso). Steamed soybeans
and toasted cracked wheat are blended together with a
starter known as “koji seed,” which is comprised mainly of
molds from the Aspergillus family. The koji is allowed to
ferment for several days, during which time the koji seed
produces proteolic, lipolytic, and amylolytic enzymes that
convert proteins, fats, and starches to simpler and more
easily fermentable substances.
2. Transferring the koji to fermentation tanks (containing a
saltwater solution), where a starter culture of lactic acid
bacteria and yeasts is added and allowed to ferment for six
months (or longer for the highest‐quality shoyu) at approx-
imately 85°F (29°C). During this time, more than 200 flavor
compounds are produced.
3. Separating the raw sauce from the remnants of the koji by
filtering it through layers of cloth. This liquid is pasteur-
ized to prolong shelf life and to form additional flavor com-
pounds, then bottled.32
Cheap soy sauce is made from hydrolyzed soy protein, but
it does not have the natural characteristics developed during
traditional fermentation and is artificially colored and
flavored. The sensory characteristics of the many different
types of genuine soy sauce, such as Indonesian, Malaysian,
FIGURE 11.13 Soy sauce options.
Type Liquid Dehydrated Granulated Low Sodium Clear
No
Preservatives
Description Dark, rich, salty,
umami
Spray dried, light
tan, free‐flowing
powder
Light tan,
granular powder
37% less sodium Light amber
colored liquid
No benzoate or
sorbate added
Usage Flavor enhancer Flavor enhancer Flavor enhancer To reduce overall
sodium
Light colored
foods
All natural food
applications
Advantage Adds color if
desired
Can replace
HVP or MSG
Eliminates
dusting in
operations
Soy sauce not
chosen due to
high sodium
content
Versatility
of use
Versatility
of use
Comments Naturally
brewed
Can be 100%
natural and non‐
GMO varieties
are available
Naturally
brewed
Coupled
with other
sodium‐sparing
technologies to
reduce overall
sodium
Naturally
brewed
Can be important
dependent
on labeling
restrictions
Food
applications
Prepared
entrees,
containing
meat, poultry,
vegetables
Marinades, dry
mixes, seasoning
blends, meat
rubs
Any foods
requiring
dry formula
applications
Soups, sauces,
dressings,
meat entrees,
vegetables,
mimic bouillon
Soups, sauces,
salad dressings,
spreads
All natural
designated foods
Source: Kikkoman.com, 2009.
c11.indd 216 1/13/2016 7:55:05 AM
CHAPTER 11 • Fermentation • 217
Japanese, Korean, and Vietnamese, are each tied to their
country of origin.
Soy sauce is available in many forms for the product devel-
oper, including light and dark varieties, reduced‐sodium,
decolorized, and powdered. It is used to enhance the flavor
profile of many types of food, including chicken and beef
entrées, soups, pasta, vegetables, and even desserts, when
used at very low levels. The flavor‐enhancing property, or
umami, of fermented soy products is used by food product
developers to blend and balance flavors. Umami compounds
are created during fermentation, when amino acids are bro-
ken down to their constituent peptides, particularly gluta-
mates, inosinates, and guanylates, all of which are flavor
potentiators—chemicals that are capable of supplementing,
enhancing, or modifying the flavor of foods, although they
have little or no flavor of their own at typical usage levels. The
effect of flavor potentiators is also accompanied by changes
in the mouthfeel of the product thereby inducing a percep-
tion of enhanced richness.
Vanilla Fermentation
Pure vanilla (made from the fermentation of vanilla beans) is
one of the most widely used flavorings in confections, baked
goods, desserts, and pastries. Pure vanilla is the second most
expensive spice flavoring in the world, next to saffron,
because growing the vanilla seed pods is labor‐intensive.
Even so, it is highly prized throughout the culinary and fra-
grance manufacturing worlds.
Historically, the bean was produced in a pod by an orchid
(Vanilla planifolia), native only to Central America, that was
naturally pollinated by a certain species of bee (also native to
Central America). This orchid opens just 6 to 8 hours per day,
restricting opportunities for natural pollination. It was only
after the introduction of hand pollination that these orchids,
and the resulting vanilla beans, could be propagated commer-
cially, allowing for global cultivation. That said, vanilla is the
world’s most labor‐intensive agricultural crop, which is why it’s
so expensive. It takes up to three years after planting before the
first flowers appear, which then must be hand pollinated. The
fruits, which resemble big green beans, must remain on the vine
for nine months in order to completely develop their signature
aroma. However, when the beans are newly harvested, they
have neither flavor nor fragrance. They only develop these dis-
tinctive properties during the curing process.
Today, 70 percent of the world’s pure vanilla is manufac-
tured in Madagascar and Indonesia.33 Other countries include
Guatemala, Costa Rica, Uganda, China, India, Papua New
Guinea, Tonga, Fiji, Tahiti, and the Philippines. The Mad-
agascar variety is commonly known as Bourbon or Mad-
agascar vanilla, and is considered to be the highest quality.
There are also Mexican and Tahitian versions, which have
their own distinctive flavor and aroma qualities.
Once the flower is pollinated, it develops a pod, 8 to 12
inches long, containing the vanilla bean, which after picking
undergoes a fermentation process developed originally by
the Aztecs (referred to as the Mexican process). This begins
by soaking the beans (stripped from the pod) in hot water,
then removing them and allowing them to dry during the
day. The beans are covered with blankets at night to allow
them to sweat. This continues for up to 10 days and con-
cludes when the beans turn dark brown and develop a white
crystalline coating on the surface. This substance is vanillin,
the compound that gives vanilla its characteristic aroma
and flavor. It is the result of naturally induced enzymatic
breakdown by beta‐glucosidase (enzyme) of the compound
glucovanillin into vanillin and sugar. While most flavor sci-
entists attribute the source of the enzyme to some strain of
bacteria, the specific microorganism responsible for the fer-
mentation of vanilla beans is unknown. At the conclusion of
the fermentation process, the beans are dried for three to
four weeks, dropping the moisture content from 60 to 70
percent to 25 to 30 percent. Finally, the beans are aged in
airtight boxes for several months to intensify their aroma
and flavor.
Vanilla extract, the most popular form of vanilla used
today, is made by steeping the aged vanilla beans in a 35
percent alcohol and water solution (13.35 ounces of vanilla
beans per gallon is the Standard of Identity set by the FDA for
pure vanilla). In the industry, this is known as single‐fold
vanilla. Fold refers to the relative measure of strength of
vanilla extract. Single‐fold vanilla is typically what the con-
sumer buys at the supermarket. For food processors, two‐,
three‐, or four‐fold vanillas are typically used. Where single‐
fold vanilla contains the extractive matter of 13.35 ounces of
vanilla beans, two‐fold uses 26.7 ounces of vanilla beans, con-
tains twice as much extractive matter, and is twice as strong.
Three‐fold and four‐fold are 3 and 4 times the content of sin-
gle‐fold. In addition, both whole and ground pods are com-
mercially available, as is vanilla paste (vanilla seeds suspended
in thick syrup).
In addition to its use as a characterizing flavor (the flavor
named on the food package—for example, vanilla ice cream,
vanilla wafers), vanilla is an effective potentiator (enhancer)
for many other flavors. Typically used at low, even subliminal
doses, vanilla boosts chocolate, coffee, and nut flavors as well
as fruit flavors. When used with citrus flavors, it tends to
cover the acid bite and makes them seem quite creamy. That
said, the dairy industry uses a large percentage of the world’s
vanilla in ice creams, yogurt (fresh and frozen), and other fla-
vored dairy products. Despite all the wonderful ice cream
choices available in the market place, vanilla is still the num-
ber one flavor.34
It is important to note that the vast majority of vanilla‐fla-
vored products actually contain synthetic vanillin, which is
much cheaper than the real thing. Culinology® professionals
should be careful to purchase vanilla extract labeled “pure” if
that is what they are looking for. If the product is labeled
“vanilla flavoring,” it is a mixture of pure and imitation vanilla
extracts.
c11.indd 217 1/13/2016 7:55:05 AM
218 • CULINOLOGY®
Biotechnology
Applications in
Fermentation
Fermentation is also used to develop food ingredients previ-
ously made by chemical reactions. Moving to a biological
approach has made it possible to simplify manufacturing and
produce cleaner labels.
Vitamin Production
Vitamin B2 (riboflavin) is essential for growth and the pro-
duction of red blood cells, and for the absorption of iron and
vitamin B
6
. Traditionally it was produced from glucose fol-
lowing a complex multi‐step chemical process. Recently it
has been produced using fermentation in a one‐step process
where vegetable oil is converted by a specific yeast to pro-
duce vitamin B2. This has greatly improved the ecological and
environmental impact in terms of raw material toxicity, per-
formance, emissions reduction, and waste disposal.35
Industrial Chemicals
Citric acid, commonly used as an acidifier and flavoring,
especially in beverages, is a six‐carbon tricarboxylic acid first
isolated from lemon juice. It is commercially produced by the
fermentation of molasses with the mold Aspergillus niger.
After the mold is filtered out of the fermentation liquid, the
citric acid is separated by precipitation with lime (calcium
hydroxide) to produce a calcium citrate salt. The salt is
treated with sulfuric acid to generate the citric acid. This
process has been in use since the early 1900s.
Lactic acid can, of course, be produced by the fermen-
tation of carbohydrates using many strains of bacteria.
However, the most common method, on an industrial scale,
uses yeast, not bacteria. In 1994, a method for the production
of lactic acid was introduced using a strain of Saccharomyces
cerevisiae that was metabolically engineered to produce lac-
tic acid rather than ethanol from sugar. Lactic acid is used in
foods and beverages as a pH regulator or as a preservative. It
is also used as a flavoring agent.
Xanthan gum, heavily used in the food industry as a
thickener and a stabilizer system in foods such as pickle rel-
ish and sauces, is the most important microbial polysac-
charide‐derived hydrocolloid, and it is widely used in other
areas, such as the pharmaceutical industry. It is made from
the fermentation of corn sugar by a strain of bacteria,
Xanthomonas campestris, known to cause black rot on broc-
coli, cauliflower, and other leafy vegetables. This bacterium
forms a slimy coat during fermentation that has the same
characteristics as gelatinized cornstarch. This substance is
isolated, filtered, and dried to yield the product known as
xanthan gum.
Conclusion
The field of fermentation technology has opened a new era
in the development of world‐class foods and ingredients
that will be used to enhance our lives for years to come. It
seems a long way from its prehistoric start, but the process
of fermentation is still the same. The only differences are the
organisms and the substrates acted on. It is up to Culinology®
researchers to continue this evolution as we move into the
future.
1P. E. McGovern, Ancient Wine: The Search for the Origins of Viniculture
(Princeton, NJ: Princeton University Press, 2003), 7.
2K. H. Steinkraus, ed., Handbook of Indigenous Fermented Foods (New York:
Marcel Dekker, 1995).
3L. Ziedrich, Joy of Pickling (Boston: Harvard Common Press, 1998), 4.
4H. McGhee, On Food and Cooking: The Science and Lore of the Kitchen, rev. ed.
(New York: Scribner, 2004), 517.
5Steinkraus, Handbook of Indigenous Fermented Foods.
6P. Debré and F. Elbong, Louis Pasteur (Baltimore: JHU Press, 2000), 106–107.
7M. Battcock and S. Azam‐Ali, “Fermented Fruits and Vegetables: A Global
Perspective,” FAO Agricultural Services Bulletins 134, ch. 5, part 5.7.1 (1998).
8M. J. Pelczar and R. D. Reid, Microbiology, 3rd ed. (New York: McGraw‐Hill,
1972), 116.
9M. Selig M., K. Xavier K., H. Santos H., and P. Schönheit, “Comparative
Analysis of Embden‐Meyerhof and Entner‐Doudoroff Glycolytic Pathways
in Hyperthermophilic Archaea and the Bacterium Thermotoga,” Archives
of Microbiology 167 (1997): 217–232.
10www.ILovePickles.org (2013).
11R. Buescher, M. Hudson, and J. Adams, “Inhibition of Polygalactouronase
Softening of Cucumber Pickles by Calcium Chloride,” Journal of Food
Science 44, no. 6 (1979): 1786–1787.
12J. G. Holt, et al., eds., Bergey’s Manual of Determinative Bacteriology, 9th ed.
(Baltimore, MD: Lippincott Williams & Wilkins, 2000).
13Battcock and Azam‐Ali, “Fermented Fruits and Vegetables.”
14McGhee, On Food and Cooking.
15Steinkraus, Handbook of Indigenous Fermented Foods.
16A. Haug, A. T. Høstmark, and O. M. Harstad, “Bovine Milk in Human
Nutrition: A Review,” Lipids in Health and Disease 6 (2007): 25, 34.
17D. B. Fankhauser, Ricotta Making Illustrated (2006), retrieved October 8, 2009,
from http://biology.clc.uc.edu/fankhauser/Cheese/Ricotta/ricotta_00.hym.
18J. Lablee, “Cheese Manufacture,” in Cheesemaking: Science and Technology
(New York: Lavoisier, 1987), 406–412.
19Ibid.
20McGhee, On Food and Cooking.
21K. Incze, “Raw Fermented and Dried Meat Products,” Fleischwirtsch 72, no.
1 (1992): 58.
22http://www.fsis.usda.gov/wps/wcm/connect/ca7ffa3c‐f9ee‐4eae‐94ee‐
bddfae623fda/33_IM_RTE_SS_Process ?MOD=AJPERES\.
23Retrieved October 8, 2009, from http://www.nysaes.corness.edu/necfe/.
24“Production of Fermented Sausages with Chr. Hansen Starter Cultures,”
BactofermTM Meat Manual (2003), 135–143.
25J. D. Owens and L. S. Mendoza, “Enzymatically Hydrolyzed and Bacterially
Fermented Fishery Products,” Journal of Food Technology 20, no. 30 (1985):
273–293.
c11.indd 218 1/13/2016 7:55:05 AM
CHAPTER 11 • Fermentation • 219
26C. S. Pederson, Microbiology of Food Fermentations (Westport, CO: AVI
Publishing, 1971), 110.
27M. Griffin, “Coffee Processing” (1999), retrieved August 20, 2009, from
http://www.coffeeresearch.org/agriculture/processing.htm.
28“Coffee Trade Statistics,” retrieved October 21, 2009, from http://www
.ncausa.org.
29S. Fallon and M. Enig, “Cinderella’s Dark Side,” Nexus 7, no. 3 (April‐May
2000): 1–3.
30K. Shiroki, “Benefits of Natto” (2003), retrieved September 9, 2009, from
http://www.gala21.net/natto/benefits.htm.
31T. Yokotsuka, “Fermented Protein Foods in the Orient, with Emphasis on
Shoyu and Miso in Japan,” in Microbiology of Fermented Foods vol. 1, B. B.
Wood, ed. (London: Elsevier Applied Sciences, 1985), 197–247.
32http//www.kikkoman.com/soysaucemuseum/index.shtml.
33http://faostat.fao.org/site/339/default.aspx.
34http://vanilla.servolux.nl/vanilla_facts.html.
35E. J. Van Damme, ed., Biotechnology of Vitamins, Pigments and Growth
Factors (London: Elsevier Science Publishers Ltd., 1989).
c11.indd 219 1/13/2016 7:55:05 AM
220 • CULINOLOGY®
12
Food Additives
Introduction
There are thousands of ingredients used to make foods. Food
additives cover a wide range of nutritive and non‐nutritive
substances that are added to foods to improve or modify
their characteristics and properties. Their use is regulated
under the Code of Federal Regulations, Title 21 (CFR 21 170.3)
of the U.S. Food and Drug Administration (FDA), which
defines their nature, proposed use, and application levels.
The FDA approves the safety of all food ingredients, but it is
the manufacturer ’ s responsibility to prove to the FDA that
any new additive (or an approved one used in a novel applica-
tion) is safe. This legislation does not apply to the 373 gener-
ally recognized as safe (GRAS) products that do not require
prior FDA approval because they have already been judged
safe through decades of commercial usage. Some of the
materials on the list are quite well known, such as soy sauce,
guar gum, and sugar; others, such as sodium ferricitro-
pyrophosphate (used to fortify the iron content of foods), are
probably unrecognizable by the general public but well
known to food scientists and government regulators. The
Food and Drug Administration (FDA) maintains a list of
more than 3000 ingredients in its database “Everything
Added to Food in the United States.” In this chapter, we have
only included a representative assortment of food additives
that are most commonly encountered by the product devel-
oper. The complete list is available on FDA ’ s website.
Food Additives
for Function
According to FDA regulations, the purposes of food additives
must fall into one (or more) of the following categories:
• Improve the appeal of foods by improving their flavor,
smell, texture, or color.
• Extend storage life.
• Maximize performance.
• Protect nutrient value.
Their typical application level usually ranges from a few
parts per million (ppm) to 1 to 2 percent.
Sequestrants and Antioxidants
Sequestrants and antioxidants help prevent the oxidation
(breakdown by exposure to oxygen) of foods. Oxygen can
cause many problems in a wide variety of food products. It
can trigger enzymatic browning in fruits and vegetables, fad-
ing of color pigments such as carotenoids, and rancidity in
fats and oils through the degradation reactions commonly
referred to as autoxidation.
Sequestrants bind free metal ions to prevent them from
catalyzing the oxidation reaction. Many metal ions naturally
present in foods (such as iron in meat products and magne-
sium in vegetables) can be released during processing.
Sequestrants are chelating agents , which trap metal ions,
preventing them from taking part in the reaction. Natural
sequestrants include malic, tartaric, phosphoric, and citric
acids. Of the natural sequestrants, phosphoric and citric
acids are used the most in food systems, especially beverages.
Remember when determining use rates that these are acids
and as such contribute sourness to the finished product.
Taste as you go.
One of the best‐known synthetic chelating agents used is
EDTA (ethylenediamine‐tetraacetic acid). It promotes color
retention in dried bananas, canned beans and chickpeas,
canned clams, pecan pie filling, frozen potatoes, and canned
shrimp. It improves flavor retention in canned carbonated
beverages, salad dressings, mayonnaise, margarine, and
sauces. It inhibits rancidity in vegetable oils, salad dressings,
mayonnaise, sauces, and sandwich spreads. EDTA salts are
Lead Author: Andres V. Ardisson Korat , MS, CCS®, Graduate Student , Harvard University
CRC®, CEC, Executive Research Chef , Applebee ’ s
Certified Flavor Chemist
Melissa Haupt ,
Jerome Lombardo ,
c12.indd 220 1/22/2016 1:12:58 PM
CHAPTER 12 • Food Additives • 221
used in foods at levels ranging from 33 to 800 ppm (800 ppm
= 0.08 percent).
Antioxidants hinder the binding of oxygen to prevent
enzymatic browning in fruits and vegetables. They do this
by scavenging oxygen free radicals to slow the rate of
reaction. In the simplest form of this process, lemon or pine-
apple juice is used to prevent the enzymatic browning of
avocado slices or cut apples. Lemon juice contains ascorbic
acid (vitamin C), which acts as a barrier between the oxygen
and the food product. The oxygen binds with the ascorbic
acid, oxidizing it instead of the food pigment.1 In larger‐scale
food processing operations, ascorbic acid is added to fruit
syrups and used to coat the outside of cut vegetables to pre-
vent browning. Pineapple juice also works as an antioxidant
because its sulfhydryl group can bind with oxygen.2 In a
bench or kitchen setting, a 3:1 ratio of water to pineapple
juice works well on cut fruits and vegetables, though its use
is restricted in commercial products because of some
people’s sensitivity to sulfites.
To prevent lipid oxidation in foods from meat products to
nuts, developers add natural polyphenolic compounds such
as tocopherols (vitamin E) and synthetic phenolic antioxi-
dants such as BHA (butylated hydroxyanisole). These are
used in a variety of food products, from potato chips and
crackers to polyunsaturated oils, chicken nuggets, and pre-
cooked hamburgers. The antioxidant gives up its own hydro-
gen atoms to the double bonds of the unsaturated fatty
acids—essentially a small‐scale hydrogenation—to halt
autoxidation. When choosing an antioxidant, it is important
to consider labeling, cost, and impact on the flavor and shelf
life of the product.
Synthetic antioxidants approved for use in food include
BHA, BHT (butylated hydroxytoluene), TBHQ (tertiary‐butyl
hydroquinone), and PG (propyl gallate). BHA is a waxy white
solid that is soluble in fats and oils but not water, and pre-
vents color and flavor fading in essential oils.3 BHT is a white
crystalline fat‐soluble solid more effective in animal fats than
vegetable oils. TBHQ is a white‐to‐tan colored powder that is
most effective in preventing autoxidation in frying oils. PG is
not effective at high temperatures, so it is used primarily in
dried or fresh meat applications. These four can also be com-
bined to work synergistically. For example, BHT and BHA in
combination are more effective in nut products than either is
alone. Synthetic antioxidants are usually more cost‐effective
than natural ones, and often more reliable, consistent, and
easier to use. However, synthetic antioxidants are regulated
by the FDA with maximum levels allowed in food, and they
must be listed on product labels, which some consumers per-
ceive negatively.
Antioxidants can also be used in food packaging to prolong
shelf life; BHT is commonly used in the bags or liners inside
breakfast cereal boxes to prevent oxidation. Antioxidant
packaging provides a controlled release of the antioxidant to
maintain a constant level over time so excessive additives are
not in the food.
Among the natural antioxidants, mixed tocopherols are
commonly used. These are eight naturally occurring com-
pounds (a mixture of tocopherols and tocotrienols) related to
vitamin E that are commercially extracted from vegetable
oils. They can be used in both vegetable and animal fat appli-
cations at a maximum level of 0.03 percent of the fat content,
according to U.S. Department of Agriculture (USDA) guide-
lines. Not all of the tocopherols have the same antioxidant
powers. High‐alpha species are usually used in vitamins,
while the low‐alpha type have better antioxidant abilities in
food.4 Both tocopherols and the synthetic antioxidants
behave in similar ways, though tocopherols can stand high‐
heat processing better because they are less volatile.
Tocopherols also have the benefit of being recognized by the
consumer as vitamin E, so there is the perception of a cleaner
label.
Herbs and spices such as clove, sage, oregano, rosemary,
and garlic also show some success as antioxidants. The raw
spices seem to be more effective than their extracts. That
said, the flavors and odors they impart can be unwanted in
some applications.5 Deodorized versions of sage, rosemary,
and garlic extracts are heavily used in the poultry industry.
Rosemary extract especially is widely used across the meat
industry, not only in poultry products but in fully cooked beef
and pork, to prevent meat flavor deterioration (MFD) or
warmed‐over flavor (WOF). MFD produces flavors that are
perceived as off, like cardboard or refrigerated taste, caused
by oxidation of fats when a meat product is cooked, cooled,
and reheated.
Herb and spice extracts also have the advantage of being
recognizable by consumers on labels. Spice extracts can be
used in combination with all other antioxidants and are not
limited in the amounts that can be added to foods.
FIGURE 12.1 FDA maximum permitted levels of
antioxidants in various food products.
Type of Food
Parts per Million (allowed of
total BHA or BHT alone or
in combination to be added)
Dehydrated potato shreds 50
Active dry yeast 1000 (BHA only)
Beverages and desserts
prepared from dry mixes
2 (BHA only)
Dry breakfast cereals 50 (BHA only)
Dry glazed fruit 32 (BHA only)
Dry mixes for beverages
and desserts
90 (BHA only)
Emulsion stabilizers for
shortening
200
Potato flakes 50
Potato granules 10
Sweet potato flakes 50
Source: FDA, 21 CFR 172.110.
c12.indd 221 1/14/2016 7:32:52 AM
222 • CULINOLOGY®
Bulking Agents
Bulking agents are used to replace or create bulk in reduced‐
sugar and reduced‐fat food products, and as fillers to dilute
extremely concentrated ingredients like high‐intensity sweet-
eners and flavors. In regular confectionary products, sugar
and corn syrup represent most of the bulk (body) of the
product. Therefore, when creating a sugar‐free or artificially
sweetened product, a bulking agent must be added to replace
the volume of sugar omitted. Sugar alcohols, also called poly-
ols (sorbitol and lactitol are two examples), are used as bulk-
ing agents in conjunction with high‐intensity sweeteners in
sugar‐free confectionery products. Bulking agents such as
maltodextrins (white, powdered, digestible carbohydrates
made from cornstarch; may be moderately sweet or virtually
flavorless) are also used as diluents with high‐intensity sweet-
eners to create products such as Splenda® (the combination
of sucralose and maltodextrin) to make the product equiva-
lent in weight and sweetness to table sugar.6 Maltodextrins
are also commonly used in the flavor industry for diluting
extremely concentrated flavors to make them easier to use;
they also work as an absorbent plating medium to convert
FIGURE 12.2 Amounts of antioxidants allowed
in meat products.
Type of Meat
Product
BHA/BHT/PG/
TBHQ* Tocopherols
Dry sausage 0.003% based on
total weight or
0.006% total if
combined with
another antioxidant
0.003% based on
fat content weight;
not to be used in
combination with
other antioxidants
Rendered
animal fats or
combination
with vege-
table fat
0.01% of finished
product or 0.02%
if combined
with another
antioxidant
0.03% not to
be used in
combination with
other antioxidants
Fresh pork
sausage,
cooked beef
patties, pizza
toppings
0.01% based on fat
content or up to
0.02% if combined
with another
antioxidant
0.003% based on
fat content weight;
not to be used in
combination with
other antioxidants
Dried meats 0.01% of total
weight of the
product alone or
in combination with
another antioxidant
0.003% based on
fat content weight;
not to be used in
combination with
other antioxidants
Poultry
products
0.01% based on
total fat content
or 0.02% if used in
combination
0.03% based on
total fat content
or 0.02% if used in
combination with
another antioxidant
except TBHQ
*TBHQ is only allowed to be used in combination with BHA or BHT
or a combination of the two.
Source: FDA, 9 CFR 424.21.
liquid flavor oils into free‐flowing powders. They are also
used to provide bulk in packaged dry mixes, especially in sin-
gle‐serve soup, sauce and gravy packets, and beverage
envelopes.
Polydextrose is a bulking agent that is an indigestible syn-
thetic polymer synthesized from dextrose (glucose), plus
about 10 percent sorbitol and 1 percent citric acid. Poly-
dextrose is commonly used as a replacement for sugar, starch,
and fat in commercial beverages, cakes, candies, breakfast
cereals, dessert mixes, frozen desserts, puddings, and salad
dressings. Polydextrose solutions are slightly more viscous
than sucrose at the same concentrations; thus, they are a use-
ful substitute in sugar‐free syrups, where viscosity similar to
sucrose is desired. Polydextrose is also used as a humectant (a
hygroscopic substance that helps keep moisture in foods), sta-
bilizer, and thickening agent. Its use is restricted in the United
States by the CFR, and, as with many sugar alcohols, more
than 15 g per serving can cause gastrointestinal distress in
some individuals.
Bulking agents made from carbohydrates and proteins are
also used to replace fat in fat‐free products. Carbohydrates
are used most frequently because of their lower cost. The
functional properties carbohydrates provide as fat replacers
include gelling, thickening, and stabilization. Starches such
as pre‐gelatinized waxy cornstarch are used in low‐fat muf-
fins and pastries for their ability to form gels that mimic the
mouthfeel of fat. Maltodextrins are used in cold applications
like salad dressings because they provide a smooth mouth-
feel without much impact on taste. Fiber is also used because
it provides structure, stability, and volume in baked goods
and beverages, and it is perceived as a healthy ingredient.
Sugar alcohols and hydrocolloids can also be used as bulking
agents to replace fat in frozen desserts, salad dressings, and
chewing gums.
Proteins can also be used as bulking agents, either alone or
blended with other ingredients. Whey, including refined prod-
ucts such as whey protein concentrates and whey protein iso-
lates, provides some of the functions of fat in systems. Whey
can also act as an emulsifier with gelling applications in
mayonnaise‐type products, salad dressings, and frostings.
Products like Simplesse® are microparticulated proteins
(MPP), which refers to the small particle size (about 1 micron
in diameter) of the protein. The Simplesse® particle size allows
it to behave almost identically to fat globules. Microparticulated
whey proteins behave like fats and can be used as fat substi-
tutes in dairy product such as cheese, creamy desserts, and ice
cream, where they greatly improve texture and taste.
Colors
Colorants in food products influence the preferences of con-
sumers. Colorants are added to prevent color loss due to
exposure to air, light, and moisture through processing or
storage; to give consistency to products with natural color
variances; to make a product more visually appealing; and to
add color to a usually colorless product (like pink to marsh-
mallow bunnies) to increase consumer appeal. Colorants can
c12.indd 222 1/14/2016 7:32:52 AM
CHAPTER 12 • Food Additives • 223
be classified as natural colors, which are pigments naturally
extracted from plants and animals, and synthetic colors,
which are designated FD&C (Food, Drug, and Cosmetic) and
therefore regulated by the FDA. Synthetic colors can be fur-
ther classified into dyes, which are water‐soluble, and lakes,
which are oil‐soluble and are called FD&C aluminum lakes.8
Dyes are used to color beverages, mixes, and dairy products.
Lakes are used to color dry ingredients and foods with a high
fat content.
Natural Colors
Natural colors are typically less stable and less consistent
than their synthetic counterparts, and usually more expen-
sive. However, the recent trend toward cleaner labels is the
main driver for their increasingly widespread use. Although
natural colors are sourced from nature, the addition of any
color to food is considered artificial by the FDA, and there-
fore products that contain them cannot claim to be made
FIGURE 12.3 Examples of bulking agents and
usage areas.
Type of Bulking
Agent
Example
of Product Usage
Polyols Many brands (1.5–3 kcal/g) Used
to replace bulk in
fat‐free products
Maltodextrin
can be made
from corn,
tapioca, or rice
solids
Maltrin®
GPC
(4 kcal/g) To increase
volume in low-fat
applications such
as fat‐free deserts
or other cold
applications
Polydextrose Litesse®
Danisco
(1 kcal/g) Used in
cakes, chocolates,
hard candies, and
beverages
Starches from
potato, corn,
rice, oats, wheat,
and tapioca
Stellar®
Staley
(1– 4 kcal/g) Used as
fat replacers, texture
modifiers, and
bulking agents in
frostings, sauces,
meats, dairy products,
and baked goods
Powdered
cellulose
Justfiber®
International
Fiber
Products
(0 kcal/g) Used in
breads, cakes,
cheese, and salad
dressings
Microparticulated
protein (whey
protein or egg
protein)
Simplesse®
NutraSweet
Co.
(2 kcal/g) Used to
increase volume in
low‐fat applications
such as fat‐free
desserts or other cold
applications
Source: Vaclavik and Christian (2008).7
with natural colors on the label. Instead, they must be labeled
by name: annatto, caramel, and so on (CFR 21 70.3).
Regardless, the increasing consumer rejection of artificial
colors often makes using the natural ones a more acceptable
choice.
The main color substance in annatto is the carotenoid
pigment bixin, found in the coating of the annatto seed Bixa
orellana (CFR 21 70.30). It is primarily soluble in fat, although
it is also available in a water‐dispersible form.9 Its color varies
from yellow to orange, depending on the application level. It
provides good color intensity and is stable to oxidation, pH
changes, and temperature. Annatto exhibits poor stability to
light.10 It is widely used in cheese production, providing
Cheddar and American cheeses with their characteristic yel-
low hue. It can be used in cheese sauces and other products
where a yellow color is desired, such as margarine (which is
white in its native state), packaged yellow rice mixes, pack-
aged custard powder, and smoked fish.
Beet color or beet juice is extracted from red beets and is
composed of betalains, water‐soluble pigments that exhibit
a deep red‐purple color in acidic conditions (pH < 6) but
become blue when the pH is neutral to alkaline.11 The most
heavily studied betalain is betanin, also called beetroot red.
Betanin degrades when subjected to light, heat, and oxygen;
therefore, it is used in frozen products, products with short
shelf life, and dry products. Betanin can survive pasteuriza-
tion when used in products with high sugar content. The
most common uses of betanin are in coloring ice cream and
powdered soft‐drink beverages; other uses are in sugar con-
fectionery—for example, fondants, sugar coatings, and fruit
or cream fillings. Betanin is also used for coloring meat,
bacon, and sausages as well as frozen soups and tomato
products.
A precursor of vitamin A, beta‐carotene provides yellow to
orange color with good color intensity. It has good stability to
pH changes, fair stability to light, and poor stability to oxida-
tion and microbial attack, which cause color loss. It is insolu-
ble in water, but oil‐dispersible forms are available. It is used
in margarine, cheese, oils, and products with high oil content
at relatively neutral pH levels.12 It can also be used in emul-
sion form to color beverages, syrups, dairy products, and ice
cream, and in dry form in instant drink powders, baked
goods, and pasta.
Caramel color is the world’s most widely used food color-
ant. It provides yellow to reddish‐brown to dark brown color
in many foods and beverages, including colas, cocoa, soy
sauce, cereals, breads, sauces, gravies, candies, dairy prod-
ucts, beer and other alcoholic beverages, and many others. It
can be produced from the controlled caramelization (heat‐
induced browning involving pyrolysis) of a range of sugars
and starch hydrolysates (CFR 21 73.85), but is most com-
monly prepared using high‐dextrose corn syrup. It is water
soluble and available in liquid and spray‐dried forms. It is sta-
ble to light, oxidation, pH, and temperature.13 However, it
loses color in response to microbial attack.
The four classes of caramel color are distinguished by the
additives used in their preparation. The first one (I) is not made
c12.indd 223 1/14/2016 7:32:52 AM
224 • CULINOLOGY®
using sulfites or ammonia and is therefore the most natural
form, but it has the least stability. That said, because of its natu-
ralness, it is used most widely. The second class (II) allows the
use of sulfites but not ammonium salts, and is commonly used
in tea, whiskey, and brandy. The third one (III) allows the use of
ammonium compounds but not sulfites, and is used in confec-
tions, bakery products, beer and soy sauce.14 The fourth form
(IV) allows both additives, which makes it suitable for acidic
applications, such as cola beverages.
Chlorophyll is a natural green color present in all green
plants and algae, but it is primarily extracted from spinach
and alfalfa with non‐polar food‐grade solvents such as alco-
hols and acetone.15 A common form is sodium copper cholo-
rophyllin, made by substituting the magnesium ion with
copper to improve light stability, which is allowed for use in
food. It can be used in dry beverage mix applications where a
green color is desired (CFR 73.125).
Cochineal is a pigment extracted from the dry bodies of the
insect Dactylopius coccus costa, which lives on cacti growing
in Mexico and South America (Peru is the largest exporter). It
takes 155,000 insects to manufacture 1 kg dye. The active
water‐soluble substance is carmine.16 It displays a color rang-
ing from deep orange to red. Cochineal is one of the few water‐
soluble colorants that resist degradation with time. It is one of
the most light‐ and heat‐stable and oxidation‐resistant of all
the natural organic colorants and is even more stable than
many synthetic food colors, but it is sensitive to pH changes
below 3.0, and it loses intensity when attacked by microbes. It
is used in a wide range of foods, including candy, baked goods,
juices, yogurts, surimi, marinades, alcoholic drinks, bakery
products, cookies, icings, pie fillings, jams, preserves, gelatin
desserts, and Cheddar cheese and other dairy products.
Because this pigment is derived from an insect source, it can-
not be used in kosher or vegan foods.
An FDA rule effective January 5, 2011, requires all foods
and cosmetics containing cochineal to declare it on their
ingredient labels (CFR 21 73.100).
Turmeric is a colorant extracted from the turmeric root
(Curcuma longa). Curcumin is the main pigment in the extract
(CFR 21 73.600). It provides a yellow to yellow‐orange color and
is soluble in water. Turmeric has moderate stability to oxida-
tion and poor stability to light and pH changes but great heat
stability.17 It is used in canned beverages and baked products,
dairy products, ice cream, yogurt, popcorn color, cake icings,
cereals, sauces, gelatins, and so on. It is a primary ingredient in
curry powder and is used in prepared foods such as rice dishes.
Synthetic Colors
Dyes and lakes are complex organic chemicals that were orig-
inally derived from coal tar but now are made from petro-
leum. Food companies like using them because they are
cheaper, more stable, and brighter than most natural color-
ings. However, there is ongoing concern that consumption of
synthetic dyes may have adverse effects, especially on chil-
dren whose diets may include a large number of highly
colored foods such as breakfast cereals, snack cakes, and can-
dies. Dyes have been blamed for increasing rates of attention
deficit disorder, hyperactivity, learning difficulties, and aller-
gies. In addition, consumers’ growing preference for natural
foods is leading some companies to either not add colorings
at all or to switch to natural colorings.
Unlike other food additives, dyes may not be used unless
the FDA has tested and certified that each batch meets legal
specifications (colors from natural sources are exempt from
certification). One benefit of the certification process is that
it provides information about the amounts of dyes sent into
commerce each year for use in foods, drugs, and cosmetics.
Just three dyes—Red 40, Yellow 5, and Yellow 6—account for
90 percent of all dyes used. The FDA’s data show a dramatic
five‐fold increase in consumption of dyes since 1955. That
increase is a good indication of how Americans increasingly
have come to rely on processed foods, such as soft drinks,
breakfast cereals, candies, snack foods, baked goods, frozen
desserts, and even pickles and salad dressings, that are
colored with dyes.21
In the United States, seven artificial colorings are certified
for use in food. All contain the prefix FD&C, which indicates
approval for use in food, drugs, and cosmetics. These seven
are known as primary colors; when they are combined to pro-
duce other colors, those colors are then known as secondary
colors.
FIGURE 12.4 Summary of properties of common natural colors.
Name Hue Solubility Heat Stability Light Stability pH Range
Annatto Yellow to orange Water and oil Good Poor 3.5–7.5
Beet color Purple to red Water Poor Good 2.5–6.0
Beta‐carotene Yellow to orange Oil Very good Fair 3.5–7.0
Caramel color Yellow to brown Water Very good Very good 2.0–7.0
Chlorophyll Green Water Very good Very good 3.0–7.5
Cochineal/carmine Orange to red Water and oil Very good Very good 3.5–7.5
Turmeric Yellow to orange Water and oil Very good Poor 4.0–7.0
Sources: Igoe and Hui (2001)18; Fennema (2007)19; Francis (1999)20.
c12.indd 224 1/14/2016 7:32:52 AM
CHAPTER 12 • Food Additives • 225
FD&C Blue #1: Brilliant blue produces green to blue color with
good pH stability under pH 7.22 It displays poor light and oxi-
dation stability and good heat stability. It is used in beverages,
candies, and desserts (CFR 21 74.101).
FD&C Blue #2: Indigotine provides a deep blue with poor sta-
bility to light, oxidation, and pH but moderate stability to
heat. It is used in candies and pet food (CFR 21 74.102).
FD&C Green #3: Fast green is a blue‐green colorant with good
heat and pH stability (3 to 8) but poor light and oxidation
stability.23 It is used in cereals, candies, beverages, ice cream,
and baked goods (CFR 21 74.1203).
FD&C Red #40: Allura red produces a red‐yellow hue with very
good light stability, good stability in pH 3 to 8, moderate sta-
bility to oxidation, and good heat stability.24 It has a wide
range of applications, including candy, desserts, ice cream,
and beverages (CFR 21 74.340).
FD&C Red #3: Erythrosine produces a high‐intensity water-
melon red color with moderate light and oxidation stability
but poor stability to pH change; it is not recommended for
use in low pH.25 It is used in soft drinks, cereals, candies, pop-
sicles, and cake‐decorating gels. It is also used to color pista-
chio shells (CFR 21 74.1303). The lake form of Red #3 is the
only lake not allowed for use in foods (since 1990, when it was
identified as a carcinogen).
FD&C Yellow #5: Tartrazine is a yellow colorant with good stabil-
ity to heat and to pH in the range of 3 to 8. Tartrazine has moder-
ate light and oxidation stability.26 It is used in beverages, candy,
ice cream, and cereal (CFR 21 74.705). The dye form must be
declared by name in the ingredient statement of the foods con-
taining it because 1 in 10,000 people are highly sensitive to it.27
FD&C Yellow #6: Sunset yellow produces a red‐yellow hue with
good pH stability and moderate light and oxidation stability.
It is used in beverages, desserts, and ice cream (CFR 21
74.706).28
Limited‐Use Colors
The following dyes are allowed by the FDA for specific limited
applications:
FD&C Orange B (red shade) is allowed only for use in hot dog
and sausage casings. That said, batches of Orange B have not
been certified for use in the past decade or longer (CFR 21
74.250).
FD&C Citrus Red 2 (orange shade) is allowed only to color the
peels of oranges not used for processing into juice or other
products. It must not penetrate into the pulp (CFR 21 74.302).
Emulsifiers
Emulsifiers are substances that reduce the surface tension
between two immiscible phases, such as water and oil, al-
lowing them to form an emulsion. The phases can be two
liquids—again, such as oil and water—combined to make
mayonnaise or oil‐based flavor emulsions in soft drinks; a
solid and a liquid, as in the case of butter; or a liquid and gas,
as in foams (whipped cream, ice cream, and meringues). The
most common emulsions in food products involve a water‐
soluble phase and an oil‐phase that can form either an oil‐in‐
water emulsion, such as mayonnaise or cream, or a water‐in‐oil
emulsion, like butter.33
Emulsifier molecules have both a polar hydrophilic group
and a non‐polar hydrophobic group. These allow the mole-
cules to interact with both polar (water‐based) and non‐polar
(oil‐based) phases. Many emulsifiers are modified triglyceride
molecules, where one or more fatty acids are substituted by a
polar end that depends on the emulsifier. When two of the
three original fatty acids on the triglyceride molecule are left
intact, the molecule is named diglyceride, and when only one
remains, the molecule is a monoglyceride.34
Some of the main functions of emulsifiers include the inhi-
bition of starch retrogradation in baked goods, which extends
freshness by slowing staling; controlling fat crystallization in
chocolates to prevent bloom; aiding in the dispersion of dry
ingredients containing fat that are meant to dissolve in aque-
ous foods, such as whole milk powder and powdered coffee
creamers; providing lubrication in confectionery processes to
prevent sugar from sticking to equipment and tools; and aid-
ing foam formation and stabilization in the production of ice
creams and meringues. Here are some of the most commonly
used emulsifiers:
FIGURE 12.5 Synthetic color substances and permitted levels.
Name
Common
Name Hue
Solubility in Water
at 20°C (g/100 ml)
Heat
Stability
Light
Stability pH Range
FD&C Red #3 Erythrosine Blue‐red 9 Moderate Moderate 3.0–7.0
FD&C Red #40 Allura red Red‐yellow 22 Good Very good 3.0–8.0
FD&C Yellow #5 Tartrazine Yellow 20 Fair Moderate 3.0–8.0
FD&C Yellow #6 Sunset yellow Red‐yellow 19 Fair Moderate 3.0–8.0
FD&C Green #3 Fast green Blue‐green 20 Very good Good 3.0–8.0
FD&C Blue #1 Brilliant blue Green‐blue 20 Good Poor 5.0–7.0
FD&C Blue #2 Indigotine Deep blue 1.6 Moderate Poor 3.0–5.0
Sources: Igoe and Hui (2001)29; Fennema (2007)30; Francis (1999)31.
c12.indd 225 1/14/2016 7:32:52 AM
226 • CULINOLOGY®
• Citric acid esters are produced by reacting citric acid
(polar end) with mono‐ and diglycerides. They are used
as metal sequestrants more effectively than citric acid
alone and as emulsifiers in shortening systems to aid in
the incorporation of the aqueous and fat phases to form
a stable water‐in‐oil emulsion.36
• DATEM (Diacetyl Tartaric Acid Esters of Mono‐ and
Diglycerides) is a hydrophilic emulsifier used in oil‐in‐
water emulsions and as a dough conditioner in breads
and rolls, helping with softness, crust formation, and
loaf volume. It is an important additive in the formula-
tion of reduced‐fat breads, where it strengthens the
dough by interacting with gluten proteins and improv-
ing mouthfeel. Other food applications for DATEM
include creamers and chocolates.37
• Lactic acid esters are manufactured by reacting stearic
acid with lactic acid (polar end) and undergoing a reac-
tion with sodium or calcium ions to form sodium
FIGURE 12.6 Blends of artificial colorants (by weight to produce specific colors).
Color Blue No. 1 Red No. 3 Red No. 40 Yellow No. 5 Yellow No. 6
Strawberry 5 95
Raspberry 5 75 20
Lime green 3 97
Mint green 25 75
Orange 25 20 55
Grape 20 80
Black cherry 5 95
Butterscotch 3 22 57 18
Caramel 6 21 64 9
Cola 5 25 70
Chocolate 10 45 45
Licorice (black) 36 22 42
Source: Francis (1999)32.
hydrocarbon chain
nonpolar group
impossible or hard to hydrate
“hydrophobic tail”
glycerol-residue
polar group
easy to hydrate
“hydrophilic head”
H
O
O
H
C
C
H
OO
C
H
2
C
H
2
C
H
3
FIGURE 12.7 Emulsifier ambiphilic structure.
Source: Pomeranz (1985)35.
c12.indd 226 1/14/2016 7:32:52 AM
CHAPTER 12 • Food Additives • 227
stearoyl lactylate (SSL) or calcium stearoyl lactylate
(CSL), the two most predominant forms used in food
products.38 They are used in baked products as dough
conditioners, in aerated products to improve foam sta-
bility, and in creamers as emulsifiers.
• Lecithin (phosphatidyl choline) has great economic impor-
tance as an emulsifier and dispersing agent. Chemically, it is
a major phospholipid component of all cell membranes,
consisting of a mixture of choline phosphates, ethanola-
mine, and inositol as the polar groups with non‐polar lipid
structures (CFR 21 184.1400). Lecithin is commercially
extracted from soybeans, although other sources, such as
egg yolks, are also used; this is why mayonnaise contains
egg yolk. There is enough lecithin in one egg yolk to emul-
sify 15 cups oil.39 Lecithin is used in food applications such
as margarine, chocolate, whole milk powder to aid dissolu-
tion in water, cocoa powders as a wetting agent (enhances
the uniform suspension of cocoa particles throughout the
milk or water phase), and in baked goods to preserve fresh-
ness and allow air incorporation.40 It is also used in oil‐
based cooking sprays as a dispersant.
• Mono‐ and diglycerides are the most commonly used
form of emulsifiers in food products. Monoglycerides (a
single fatty acid on a glycerol backbone) are primarily lipo-
philic and are manufactured by the esterification of fatty
acids with glycerol. The mixture is then distilled to obtain
a >90 percent concentration of the monoglyceride form.41
Depending on the characteristics of the attached fatty
acid chain length and saturation level, they can be found
as liquids or solids. They are used in a wide range of prod-
uct applications, including ice cream, creamers, baked
goods, shortening, and snack foods, at levels of 0.1 to
0.3 percent. Diglycerides are emulsifiers manufactured
from the esterification of two fatty acids with glycerol or
by the controlled interesterification of triglycerides with
glycerol.42 They are found in many bakery products, coffee
creamers, peanut butter, and puddings. Using a combina-
tion of mono‐ and diglycerides allows the product devel-
oper to take advantage of both forms.
• Polyglycerol esters are made by reacting glycerol with fatty
acids to form polymeric ester chains. They can be hydro-
philic or lipophilic, depending on the extent of polymeriza-
tion. They are used in icings, whipped products, and baked
goods at application levels of 0.1 to 1 percent.
• Polysorbates (known commercially as Tween®) are a
group of hydrophilic emulsifiers obtained by reacting
sorbitan esters (made from sorbitol) with ethylene oxide.
They are used in baked products, icings, coffee creamers
(to reduce oil separation), coatings, and vitamin solubili-
zation (fat‐soluble vitamins in aqueous systems) at
levels of 0.05 to 0.1 percent. The most common forms are
polysorbate 60, 65, and 80 (CFR 21 172.836, 838, and 840).
• Sorbitan esters are lipophilic emulsifiers made from the
reaction of sorbitol and stearic acid. They are used as
emulsifiers, aerating agents in whipped products, and
lubricants in baked goods.43
• Sucrose esters are produced by reacting sucrose with
fatty acids. They are used in oil‐in‐water emulsions, such
as dairy beverages, to control crystallization and as
foaming agents. Sucrose esters are also used in baked
products, frozen desserts, and dairy desserts.44
Phosphates
Phosphates, natural compounds of the element phosphorus,
are among the most widely used food additives in processed
foods. They are used in meat applications, cheese products,
beverages, and bakery items, either alone or in combination
with other products, to perform many functions.
In the meat industry, phosphates serve to increase water‐
holding capacity (WHC), extract muscle proteins, increase
cook yield, reduce oxidative rancidity, preserve meat color,
increase flavor retention, and reduce microbial growth. Their
use is limited by the USDA to no more than 0.5 percent of the
finished product. This is just as well, as excess phosphate addi-
tion can cause soapy flavors, rubbery texture, and poor color.
Sodium tripolyphosphate (STPP), an alkaline phosphate, is the
most commonly used phosphate in meats, valued for its cost‐
effectiveness and its relatively high solubility in water.
Phosphates combined with salt increase the ionic strength of
industrial meat brines and marinades, allowing for the extrac-
tion of the myofibrillar (salt‐soluble) proteins by making them
more soluble and better able to absorb and hold water. This
also creates a sticky surface on the meat that helps bind pieces
together to make formed meat products. Phosphates also
interact with the proteins in meat emulsion products (hot
dogs, bologna, and so on) as an emulsifying agent holding
together the water, fat, and protein. Acidic or neutral phos-
phates include sodium hexametaphosphate (SHMP) and
sodium acid pyrophosphate (SAPP). These contribute to the
development and preservation of color in meat products and
have a significant impact as sequestrants. SHMP binds readily
with magnesium and calcium ions, which is beneficial when
hard water is used in processing because it prevents these ions
from catalyzing free radical formation and contributing to oxi-
dation reactions. Combinations of phosphates are used to gain
maximum water‐holding capacity while maintaining color
and the maximum sequestration impact.
When using any phosphate (or blend), always dissolve it in
cold water before adding other ingredients. Phosphate is less
successful at competing for water‐binding sites, and other
solutes (such as salt) block the phosphate from fully dissolv-
ing. If phosphates are not properly dissolved, they can create
pockets of off colors and lead to reduced water‐holding
capacity in the product.
In processed cheese, phosphates such as SHMP and STPP are
used as an emulsifying agent. By chelating the calcium bound to
casein proteins, this increases their solubility and emulsifying
properties, which helps stabilize the emulsion of water, fat, and
proteins. They may be used at up to 3 percent in processed
cheese but are usually used at lower levels (around 2 percent).45
In beverages such as colas, phosphoric acid is used to
deliver a tart note without the fruity undertones found in
c12.indd 227 1/14/2016 7:32:53 AM
228 • CULINOLOGY®
other acids. In creamers, dipotassium phosphate is used as a
buffer for the dairy component to keep it from coagulating
when mixed with the acids in coffee.46
Phosphates are also used heavily as processing aids in
the starch industry. Starch manufacturers process native
starches with phosphorus oxychloride or sodium tri-
metaphosphate.47 The resulting modified starch is cross‐
linked, yielding a starch that stands up to shear, cooking,
and low pH while maintaining better stability and
increased gelatinization in comparison to native starches.
Phosphates are also used in chemical leavening systems
(see Figure 12.8).
Chemical Leavening Agents
The most widely recognized chemical leavener (from the
Latin levare, “to rise”) is baking powder. Chemical leavening
agents work by combining an alkaline substance, such as
sodium bicarbonate, or baking soda, with an acid component
(see below) and mixing with water to create carbon dioxide
gas in non‐yeast‐leavened batters and doughs. (Baking pow-
der also contains cornstarch to prevent moisture in the air
from being absorbed by the reactants so they don’t react pre-
maturely in the container.) The carbon dioxide gives baked
products their lift and honeycomb structure.49
The four types of baking powder all contain starch
and sodium bicarbonate but differ with regard to the acid
ingredient:
1. Tartrate baking powders contain cream of tartar (tartaric
acid). These were the earliest type and are known as single‐
action baking powders because they react immediately
when wetted and go to completion quickly. If batters are
not handled speedily, much of the rising action is spent by
the time the product gets into the oven, resulting in heavy,
fallen baked goods.
2. Phosphate baking powders contain calcium or sodium
phosphate as the acid component. These better control
the rate of carbon dioxide release so more is available to
act in the oven.
3. Anhydrous phosphate baking powders contain anhydrous
monocalcium phosphate as the acid, and the powder is
treated to slow the rate at which it dissolves. Little carbon
dioxide is generated during mixing; most is released early
in the baking period. This type is primarily available for
industrial use, where it is widely used in packaged mixes.
4. Sodium aluminum sulfate phosphate baking powders con-
tain two acidic components. The phosphate component
reacts quickly during mixing, while the sulfate requires the
heat of baking in order to react. This is known as double‐
acting baking soda and is the most common type sold on
the retail market.
Regardless of type, accurate measurement of baking pow-
der is essential. Too little, and the end product is heavy and
dense; too much leads to over‐rising followed by collapse,
coarse, loose texture, and off flavor from the baking powder.
FIGURE 12.8 List of common phosphates and applications.
Phosphate Ingredient Applications Function
Sodium tripolyphosphate Cheese powders, frozen vegetables,
sausage, meat and poultry products,
seafood
Protein and emulsion stabilizer,
moisture binding, texture enhancement
Sodium hexametaphosphate Canned milk, cheese, egg whites, frozen
vegetables, meat and poultry products,
seafood
Protein and emulsion stabilizer, moisture
binding, texture modifier
Sodium acid pyrophosphate Baking powder, sausage, frozen potato
products, canned seafood
Leavening, emulsion stabilizer, color
development, chelating agent for
copper and iron to prevent discoloration
Phosphoric acid Colas Acidulant
Dipotassium phosphate Creamers Buffering agent
Sodium aluminum phosphate Cake, breads, and batters Leavening agent
Tetrasodium pyrophosphate Milks, cheese, ice cream, sausage Sustain ingredient distribution,
protein and emulsion stabilizer
Tricalcium phosphate Beverage mixes, fruit juices, cereals,
grated cheeses
Anti‐caking, fortification
Disodium phosphate Milks, cheese, ice cream, dried pastas Protein and emulsion stabilizer, decrease
cooking times
Monocalcium phosphate Baking powder, canned fruits and
vegetables
Leavening, texture enhancement
Source: Phosphate Facts (2010)48.
c12.indd 228 1/14/2016 7:32:53 AM
CHAPTER 12 • Food Additives • 229
Antimicrobial Preservatives
Antimicrobial preservatives are antimicrobial agents that play
an important role in preventing food spoilage and improving
food safety. The most common category is the group of organic
acids comprising benzoic acid, propionic acid, and sorbates,
preservatives that have been used for decades.50
Benzoic acid is one of the most common antimicrobial
agents used in the food industry. It occurs naturally in some
fruits and spices but is commercially manufactured by chem-
ical synthesis (CFR 21 184.1021). The undissociated hydrogen
form is the active component in a pH range of 2.5 to 4.0,
which makes it suitable for juices, soft drinks, pickled foods,
jams, and fruit preparations.51 The sodium salt (sodium ben-
zoate) is water soluble; it converts to benzoic acid when in
solution, which makes it preferable in many products. It is
effective against mold, yeast, and some bacteria, and it is
often used in combination with other preservatives, such as
sorbic acid, at levels of 0.05 to 0.1 percent.
Propionic acid is commonly found in the form of sodium
and calcium salts, and it is one of the characteristic acids and
FIGURE 12.9 Properties of common leavening
ingredients.
Ingredient
Type
Rate of
Reaction
Neutralizing
Value
Types of
Products
Potassium
hydrogen
tartrate
(cream of
tartar)
Fast‐acting
acid—
quickly
releases
carbon
dioxide
during
mixing
45 Biscuits and
batters where
the product
is cooked
immediately
after mixing.
Limited use
in commercial
applications
because of
fast reaction
rate and cost.
Monocalcium
phosphate
(MCP)
Fast acting 80 Used in snack
products
including
crackers and
cookies.
Sodium acid
pyrophos-
phate (SAPP)
Fast to slow,
available
in different
grades with
different
reaction
times
72 Used in cake
donuts and
some cake
mixes.
Sodium
aluminum
phosphate
(SALP)
Slow
acting
100 Most common
leavening
acid used in
double‐acting
systems.
Source: Melissa Haupt, CRC®.
flavors naturally found in ripened Swiss cheese. It is an effec-
tive antimicrobial agent against bacteria and molds because
they cannot metabolize an acid that has a three‐carbon chain
length. Due to its acid nature, it can only be used environ-
ments where the pH is under 5.0 (CFR 21 184.1081). Propionic
acid is used primarily in bakery items in concentrations up to
0.3 percent. The calcium form has little water solubility and is
used on cheese surfaces and wrapping materials. The sodium
salt is moderately soluble in water and is used in processed
cheese products.
Sorbic acid is a preservative effective against yeast and
molds in products whose pH is below 6.5. It is available as a
water‐soluble powder and can also be applied by spraying
onto foods or the packaging material. It is not recommended
for foods that undergo a significant heat treatment, as it can
break down with heat. Potassium, sodium, and calcium salts
are all available and are more soluble than sorbic acid, but
their antimicrobial activity is narrower. Sorbic acid is used in
processed cheese, beverages, pickled foods, and syrups at lev-
els of 0.05 to 0.1 percent.52
Epoxides exhibit strong, broad‐spectrum antimicrobial
activity that targets both viruses and spores. Chemically,
epoxides are cyclical ethers, of which the most common
forms are propylene oxide and ethylene oxide, which is the
strongest form.54 They are applied in their vapor state, and
the residue is removed by gas‐flushing. They are used in the
sterilization of low‐moisture foods and packaging materials.
Propylene oxide for food fumigation is regulated by CFR 40
part 185.15. It establishes a residue tolerance of 300 ppm
(parts per million) for nutmeats, cocoa powder, and spices.
Lysozyme is a bacteriolytic enzyme naturally present in
albumen (egg white), where it helps protect the developing
egg from microbial attack. It is commercially produced from
chicken eggs. Lysozyme is effective against the clostridia that
cause late blowing in aged cheeses (a defect characterized by
eyes, slits and cracks caused by the production of gas bubbles
as well as abnormal cheese flavor from butyric acid) and
helps prevent spoilage of wine by lactobacilli. It can also
inhibit growth of gram‐positive spoilage organisms and path-
ogens, including Listeria and Bacillus cereus.
Natamycin is an effective mold growth inhibitor when
applied to the surface of products such as cheese. It is produced
FIGURE 12.10 Effect of pH on dissociation of
organic acids.
pH Sorbic Benzoic Propionic
3 98 94 99
4 86 60 88
5 37 13 42
6 6 1.5 6.7
7 0.6 0.15 0.7
(pKa) 4.67 4.19 4.87
Source: Hui (1992).53
c12.indd 229 1/14/2016 7:32:53 AM
230 • CULINOLOGY®
by fermentation from the microorganism Streptomyces
natalensis.55 Natamycin is ideal for treating cheese because it
does not have an antimicrobial effect on bacteria, allowing
them to continue the process of cheese ripening.56 In order to
apply it, cheese is dipped in a solution of natamycin of 300 to
2000 ppm prior to packaging.
Nisin is a peptide with antimicrobial properties produced
by fermentation from certain strains of Streptococcus lactis.
Effective against the growth of Clostridium botulinum,57 it
is used in processed cheese products, processed fruits, and
meats (CFR 21 184.1538).
Nitrites are the sodium and potassium salts of nitrous
acid. Their main application is in cured meat products
(bacon, ham, corned beef, and so on), where they form
nitric oxide, which reacts with myoglobin to form nitroso-
myoglobin, which gives cured hams and meat products
their characteristic pink hue.58 In addition to fixing the
color, nitrites also act as a preservative, inhibiting the
growth of Clostridium botulinum, and are effective in a pH
range of 5.0 to 5.5. Nitrites in foods have the unfortunate
ability to transform into nitrosamines when heated. These
carcinogenic compounds have been found in all cured
meats, fried bacon, and even beer (as a consequence of the
malting process); as a result, the amount of nitrite added
to cured meats is strictly regulated. It is now mandatory to
add ascorbic acid (or erythorbic acid) to cured meats
to inhibit nitrosamine formation.
Parabens are esters of benzoic acid, most commonly
methyl and propyl esters. They are effective against yeast and
molds over a wider pH range than benzoates and are stable
to temperatures over 158°F (70°C). They are used in baked
goods, candies, frozen dairy products, fruit juices, jellies and
jams, marinated fish products, mayonnaise, mustard, proc-
essed vegetables, spicy sauces, beverages such as soft drinks,
and syrups.59
Sulfur dioxide (SO
2
) has been used in food products, par-
ticularly in the dehydrated fruit, molasses, and wine indus-
tries, for many years. In addition to inhibiting the growth of
microorganisms, it also helps mitigate enzymatic and non‐
enzymatic browning reactions. Its use in foods is strictly reg-
ulated because sensitive asthmatics can have severe reactions
to it (CFR 21 182.3862). Warning labels must be posted on all
foods that contain it at greater than 10 ppm.
Cultured dextrose is a natural antimicrobial produced
via the controlled fermentation of dextrose (sugar) with
Propionibacterium freudenreichii. This bacterium is best
known for its role in the creation of Swiss cheese. Propioni-
bacteria are commonly found in milk and dairy products and
have a long history of safe use in food. Cultured dextrose is an
all‐natural off‐white powder containing short‐chain organic
acids and other natural fermentation‐derived metabolites
that prevent the growth of an assortment of bacteria, yeasts,
and molds, thereby extending the shelf life of a wide range of
foods, including baked goods, cheeses, meats, salad dress-
ings, condiments, dips, spreads, and more. Cul tured dextrose
is a clean‐label alternative to chemical preserv atives like sor-
bates and benzoates.
Flavors
As the server waves an entrée past your nose and places it in
front of you, its aroma enters your nasal passages and you
begin to identify the familiar smells. When you place that
first forkful in your mouth, you smell the aroma again, and
you begin to experience other sensations in your mouth: the
sweetness and the tangy acidity of the sauce, the saltiness
and umami of the meat, the mild bitterness of the seared
meat or vegetables, and the pungency of peppers. You are
experiencing flavor.
Many authors have tried to define flavor and achieved
some agreement.60 Flavor is the sum of a mixture of the sen-
sory characteristics of a material taken into the mouth. It is
composed of gustation, olfaction, and tactile or trigeminal
sensations. Interestingly, flavor is 80 percent what we smell
and only 20 percent what we taste.
Gustation includes the five basic tastes perceived on the
tongue: sweet, salty, sour, bitter, and umami. Umami is the
brothy, meaty, or savory taste of foods. It is the taste sensa-
tion activated when we eat something containing gluta-
mates. Glutamates are the salt form of glutamic acid, an
abundant, naturally occurring amino acid. One of the most
common glutamate-containing ingredients is MSG (mono-
sodium glutamate), but glutamates are also commonly found
in dried mushrooms, aged cheese, sundried tomatoes, and
many other foods. Although Kikunae Ikeda first identified
umami in 1908, it was not until the late 1980s that it was gen-
erally accepted as the fifth basic taste.61
Olfaction is aroma, as perceived by sensory cells in the
nasal cavity. As you bring food to your mouth, you first smell
it through the nose, or orthonasally. Then, as you chew the
food, the aroma passes through the retronasal passages near
the back of the roof of the mouth and enters the nasal cavity
from behind.
Trigeminal or pain/temperature stimuli include the tem-
perature of the food, the heat of capsicum, and the cooling of
menthol.
Flavorings or flavors are additives whose primary function
is to provide or enhance the smell and taste stimuli received
when eating. Product developers add flavorings to foods and
beverages for many reasons. They may provide a character-
izing or identifying flavor to an unflavored or mildly flavored
base, such as most beverages, hard candies, surimi, or broths.
Flavors can provide the developer with an easy way to create
line extensions by “top noting” a common base (potato chips,
for example) with an unlimited number of flavor profiles
(Cajun, BBQ, Cilantro‐Lime, etc.). They may enhance or sta-
bilize a food’s native taste to replace what is lost from
processing, as in processed beef and poultry products. Some
highly desirable ingredients are expensive, and flavors are
economical extenders or substitutes. They can also replace
or extend ingredients with inconsistent quality or ingredients
that are not available year round. Agricultural food ingredi-
ents may become unavailable or expensive due to crop short-
ages, environmental problems, climatic problems, or political
troubles. In 2008, lemon juice was in short supply due to
c12.indd 230 1/14/2016 7:32:53 AM
CHAPTER 12 • Food Additives • 231
freezing weather in California and Argentina and droughts in
Spain and Australia.62 To meet the demand for lemon juice,
food manufacturers collaborated with flavor companies to
find alternatives. These included sourcing lemon juice from
uncommon regions, but also using less lemon juice and add-
ing flavors and citric acid to augment the desired flavor pro-
file, or using a de‐flavored fruit juice and adding lemon flavor.
Label constraints (“Contains 100 percent juice”) often made
it difficult to entirely replace the lemon juice.
Flavors can also be used to mask off notes or unpleasant
flavors such as the grassy note of soy, the metallic taste of
potassium chloride, and the chemical taste of synthetic
sweeteners. In general, product developers use flavors to
make foods and beverages more palatable, more desirable,
and more pleasurable to the consumer.
Natural and Artificial
Flavorings
The United States recognizes two classes of flavorings for
FDA‐regulated products: natural and artificial. Natural fla-
vorings contain flavoring substances derived from spices and
herbs, fruits or fruit juices, vegetables or vegetable extracts,
edible yeast, meats, seafood, poultry, eggs, dairy products, or
fermented products; their primary function in food is flavor-
ing rather than nutrition.63
Artificial flavor ingredients are obtained through chemi-
cal synthesis and do not meet the above definition. When
both natural and artificial flavorings are used in a food
product, it must be labeled “Contains natural and artificial
flavors.”
A range of flavor titles is used depending on the presence
of natural and/or artificial flavors and whether or not the
characterizing food ingredient is present. The characterizing
food ingredient is related to the title of the flavor; for exam-
ple, strawberry in a strawberry flavor.
The USDA regulates meat and poultry products in the
United States. Although it allows the use of the same ingredi-
ents approved by the FDA, the two agencies offer sharp dif-
ferences in labeling policy. In general, the USDA requires the
labeling of more ingredients, such as protein sources, some
processing aids, and smoke flavorings, and it limits the use of
binders, phosphates, nitrites, food acids, and colorants. Until
recently, the USDA did not distinguish between natural and
artificial flavors. In 1982, the USDA defined the term natural
in Policy Memo 055. This was rescinded and refined in 2005.
The USDA allows the use of the term natural under specific
defined conditions, including that the product does not con-
tain artificial flavors, colorings, chemical preservatives, or
any other artificial or synthetic ingredients and that the prod-
uct and its ingredients are minimally processed. This includes
traditional processes, such as smoking, roasting, freezing,
drying, and fermenting, and physical processes that do not
fundamentally alter the raw product or that only separate a
whole, intact food into component parts, such as grinding
meat, separating eggs into albumen and yolk, and pressing
fruits to produce juices.64
FIGURE 12.11 Common flavor titles chart.
Example of Flavor
Titles Used Meaning of the Flavor Title
Natural X Flavor Contains food ingredient X
and non‐flavoring ingredients
(solvents, carriers, processing aids,
preservatives, etc.).
Natural X Flavor
WONF
Contains food ingredient X,
natural flavorings (other than X),
and non‐flavoring ingredients.
Natural Flavor
(X Type)
Does not contain food ingredient X.
Contains natural flavoring and
non‐flavoring ingredients.
Artificial X Flavor Does not contain food ingredient
X. Contains artificial flavoring and
non‐flavoring ingredients.
Natural and
Artificial X Flavor
Contains food ingredient X,
natural flavorings, artificial
flavorings, and non‐flavoring
ingredients.
Natural and Artificial
Flavor (X Type)
Does not contain food ingredient
X. Contains natural flavorings,
artificial flavorings, and
non‐flavoring ingredients.
Vanilla Flavor Special labeling rules because
vanilla has a standard of identity
(Vanilla Extract, 21 C.F.R.ş 169.175
(2009)).
Source: Jerome Lombardo.
Unlike the FDA, the USDA requires that certain flavors be
manufactured under inspection. Flavors containing >2 per-
cent cooked meat, >3 percent raw meat, >10 percent poultry
skin, >10 percent poultry fat, or >30 percent animal fat are
considered amenable and require inspection. Certain combi-
nations of these ingredients or the addition of broth may also
render a flavor amenable.
More than 3000 ingredients are approved for use in flavor-
ings. These include plant‐derived extracts and distillates,
food concentrates, dehydrated foods, hydrolyzed plant pro-
teins, enzyme‐modified cheeses, synthetic materials, and
other food‐derived ingredients. This wide range provides fla-
vorists with the tools for creating an assortment of flavors for
savory, dairy, confection, bakery, beverage, oral care, and
pharmaceutical applications. Emerging market demands
place greater restrictions on the ingredients and processes
used to produce foods and food ingredients. These include
organic foods, minimally processed and all‐natural foods,
genetically modified organism (GMO) ‐free, gluten‐free,
locally grown, vegetarian, vegan, and allergen‐free. These
limit the palette of ingredients available for flavor creation,
but over time, suppliers develop new flavor ingredients to
meet market demands.
c12.indd 231 1/14/2016 7:32:53 AM
232 • CULINOLOGY®
Armed with this array of ingredients and processes, fla-
vorists create flavorings by means of two methods: com-
pounding and reactions. Compounded flavors are created by
blending a range of aromatic ingredients to achieve a desired
flavor profile. Reaction flavors, often called “proc essed fla-
vors,” are prepared by reacting or cooking flavor precursors to
create the desired profile.
To create a compounded flavor, the flavorist uses a combi-
nation of ingredients, chemical analysis (using analytical
tools such as gas chromatography and mass spectro metry),
and creative skills developed over years of practical experi-
ence to select the best ingredients. A creative flavorist dis-
sects a food into individual descriptors and then selects
ingredients that match each descriptor. For example, a fresh
tomato may be described as having fruity notes, floral notes,
sulfur notes, and green notes. The flavorist selects ingredi-
ents that deliver each of these notes while meeting regulatory
and other criteria.
When developing a processed flavor, the flavorist must
consider not only the flavor precursors but also the tem-
perature, pressure, pH, water activity, and reaction time of
the process. Often, the flavor precursors include amino
FIGURE 12.12 Glossary of common flavor ingredients.
Ingredient Definition
Absolutes Produced by dissolving a concrete in alcohol, chilling, and filtering. The alcohol is
removed by distillation. Waxes and resinous material are removed via this process.
Autolyzed yeast extracts (AYE) Prepared by the aerobic fermentation of Saccharomyces cerevisiae, followed by
autolysis and filtration to remove cell walls. The resulting product is concentrated
and may be spray dried.
Concentrate Juices from fruits and vegetables, concentrated by the removal of water.
Concretes An extract of plants, typically flowers, prepared by solvent extraction followed by
distillation of the solvent. A non‐polar solvent is typically used.
EMC (enzyme‐modified cheese) Prepared by treating cheese or other dairy products with enzymes to break down
fats and proteins. The enzymes are deactivated with heat, and the product may
be dried. The resulting product is 8 to 15 times stronger than the original cheese.
Essential oil The volatile oil obtained from plants. May be steam distilled (a heat process) or
expressed (a cold process).
HPP (hydrolyzed plant protein) Prepared by treating plant proteins (soy, corn, corn gluten, wheat, wheat gluten)
with acid to break them down to amino acids and small peptides. Neutralized
with alkali, filtered, and concentrated. May be dried by spray drying or vacuum
drying.
HVP (hydrolyzed vegetable protein) Same as HPP.
Oleoresins Prepared by extracting the volatile and non‐volatile components of an herb or
spice with a solvent, followed by distillation of the solvent.
Resinoids Prepared by solvent extraction of natural resins, followed by distillation of the
solvent. Similar to concretes, but the starting material is a plant exudate.
Tinctures, fluid extracts, and
solid extracts
A hydroalcoholic (water and alcohol) extract of a plant material. Solid extracts are
further concentrated.
Yeast extracts Same as autolyzed yeast extract (AYE).
Source: Jerome Lombardo.
acids and reducing sugars, ingredients essential for the
Maillard reaction (non‐enzymatic browning) to occur. The
Maillard reaction produces most of the common roasted
flavors we recognize in meats, cereals, coffee beans, and
chocolate. Other ingredients used in process flavor recipes
include fats, oils, meats, dairy ingredients, enzymes,
vegetables, yeasts, hydrolyzed vegetable protein (HVP), and
solvents.
Flavorists often layer flavor components, much in the way
a chef creates a culinary recipe. This is known as the flavor
pyramid approach. The foundation (or base note) provides
basic tastes and has little aroma—for example, the sweetness
and sourness of a strawberry. The middle note contains
ingredients that provide some aroma and impart more defi-
nition to the profile. Now the strawberry example tastes like
a strawberry. The top note gives the flavoring greater defini-
tion, impact, and character. Using the strawberry example,
the flavoring can be differentiated as a freshly picked mature
strawberry, a slightly green strawberry, or a cooked, jammy
strawberry.
Flavorings are available in liquid, paste, and powder forms.
The most suitable form depends on stability or shelf life
c12.indd 232 1/14/2016 7:32:53 AM
CHAPTER 12 • Food Additives • 233
The Flavor Pyramid
Top Notes:
• Provide flavor character, differentiation, and flavor impact
• Produced by compounding aromatic flavor components; encapsulation is application
dependent. May contain proprietary aroma chemicals.
• Advantage: Highly specific, intense
• Examples include compounded liquid flavors based on aromatic components
• Provide body, general sweet notes, and/or flavor enhancement
• May be produced using enzymolysis, hydrolysis, fermentation, etc.
• Advantage: “clean label” declaration (natural), flavor enhancement, body-givers
• Examples include foods, juice and fruit purees, yeast extract
Mid Notes:
Base Notes:
• Provide characteristic flavor
• May be produced by using process/
reaction flavor technology,
enzymolysis, fermentation
• Advantage: characteristic and
specific flavor profiles
• Examples include process/reaction
flavors based on reducing sugars,
precursors/amino acids, botanical
extracts
• Provide body general sweet notes and/or flavor enhancement
Mid Notes:
Base Notes:
• PrProvovididee chchararacacteteririststicic flflaa
•• MMaMayy bbebe pprorodduduceceddd bbyby uu isisinn
reaction flavor technologg
enzymolysis, fermentatio
• AdAdva tntage: chhara tcteriistitic
spe icifific flflavor profilfiles
•• ExExamamplpleses iincncluludede pprorocecess
flflavavororss babasesedd onon rrededucucinin
precursors/amino acids,
eextx racts
FIGURE 12.13 The flavor pyramid.
Source: Spicetec Flavors and Seasonings, Jerome Lombardo.
c12.indd 233 1/14/2016 7:33:02 AM
234 • CULINOLOGY®
requirements, cost, and ease of use in the final product.
Liquid flavors may be oil soluble or water soluble. Oil‐soluble
flavors are soluble in vegetable oils, usually in all proportions.
Some of the solvents used in oil‐soluble flavors include vege-
table oils, benzyl alcohol (an excellent solvent that readily
dissolves a wide range of flavor ingredients), and d‐limonene
(isolated from orange oil).
Water‐soluble flavors are soluble in water in at least the
amount necessary to impart sufficient flavor. The solvents
used include water, propylene glycol (most common), ethyl
alcohol (for example, grain alcohol), and triacetin (a versatile
solvent soluble up to 6.5 percent). Flavor emulsions provide a
way to make oil‐soluble flavors capable of being uniformly
dispersed in an aqueous end product: the flavor‐containing
oil phase is dispersed in an aqueous phase containing gums,
starches, or other hydrocolloids to maintain a uniform
suspension.
Powdered flavors are typically water soluble and oil dis-
persible. In virtually every case, they (or their components)
start out life as a liquid. The several methods of producing
powdered flavors each have differing functionality, advan-
tages, and weaknesses.65
When searching for the best flavor for a project, a product
developer must select a suitable supplier, request a flavor,
request a revision (perhaps many revisions), and, if success-
ful, prepare for manufacturing.66
When requesting a flavor sample, it is best that the devel-
oper be as open as possible about the end product to ensure
the samples submitted are suitable for their product, will sur-
vive their process, and meet their sensory, regulatory, and
cost‐in‐use needs. Key information to provide includes the
type of product to which the flavor will be added, the type of
processing extremes it will be subject to, preferred form (liq-
uid, powder, or paste), regulatory requirements and corpo-
rate restrictions, cost restraints, estimated volume, and
deadlines or project timeline. Clearly describe the desired
flavor profile. Use comparisons with known market products
or recipes to give the supplier a clear understanding of the
desired flavor profile. If possible, target products or gold
standard prototypes should be tasted alongside the flavor
supplier to help speed development.
As no two products or processes are identical, revisions of
the initial flavor submission are usually required to obtain the
most appropriate flavoring. When contacting the supplier,
describe as clearly as possible how the initial flavor submis-
sion differed from what you were looking for. Developing
common flavor descriptors is important. Tasting together
and discussing the flavor attributes helps build a common
lexicon of flavor descriptors as well as a strong working rela-
tionship. For example, Figure 12.15 shows some descriptors
for cheddar cheese, their definition, and a typical ingredient
to use as a common reference.67 Also, mention any issues
with the functionality of the flavor, like solubility or handling
difficulties.
As the new product nears plant trials or commercializa-
tion, the developer must obtain pricing and documentation,
FIGURE 12.14 Comparison of common dry flavor technologies.
Process Description Relative Cost Advantages Weaknesses
Plating Oil‐soluble flavorings are
coated on a crystalline
substrate, such as salt,
starches, and dextrins
(derived from starch).
Low Low cost. May be produced
in any type of blender.
Suitable for spices and
extractives.
Volatile flavorings
(flavorings that vaporize
at low temperatures) will
ultimately evaporate.
Formation of off flavors is
possible due to oxidation.
Spray drying An oil‐soluble flavor is
dispersed in a gum or
carbohydrate solution and
sprayed into a chamber
where it is dried with heat.
Moderate Most common method.
Volatile flavors are “trapped”
in the gum/carbohydrate
carrier. Moderate protection
from oxidation.
Moderate to poor
protection from oxygen.
Vacuum drying A flavor paste containing
mostly protein is dried
with heat under vacuum.
Moderate Roast notes are produced
during drying. Good for
meat and poultry flavors.
Most volatile flavors are
lost during process. Labor
intensive.
Glassy matrix An oil‐soluble flavor
is encapsulated in a
carbohydrate matrix
that is in a glassy state
(e.g., hard candy).
High Excellent oxidative stability. Low flavor loads. Highly
volatile flavors are lost
during processing. Cost.
Gelatin
encapsulate
Droplets of an oil‐soluble
flavoring are encased
in a gelatin shell.
High Excellent heat stability. Cost. Inconsistent
flavor release in food
applications.
Source: Jerome Lombardo.
c12.indd 234 1/14/2016 7:33:02 AM
CHAPTER 12 • Food Additives • 235
if this was not done earlier. Now that a recipe is nearly com-
plete, the developer must confirm the volume and request
pricing. The developer needs several documents from the
flavor supplier:
• An FDA ingredient statement or USDA label is needed
to complete the final product’s ingredient statement and
to write the specifications for the new material.
• A nutrition facts label is required under the 1990 Nutrition
Labeling and Education Act (NLEA).
• A Material Safety Data Sheet (MSDS) contains han-
dling information, hazards, if any, and flashpoint
(temperature where the mixture could ignite); U.S. law
requires it.
• The Continuing Guarantee states the product is manu-
factured under sanitary conditions, packaging materials
are approved for food contact, and government regula-
tions are met.
• A Natural Certification, if applicable, states the flavor
does not contain any artificial flavoring ingredients as
defined in 21 CFR 101.2. Non‐flavoring ingredients, such
as solvents, carriers, emulsifiers, antioxidants, and proc-
essing aids, used in the flavor may not be natural. This
is important if the product is to have an all‐natural
claim.
• A kosher certificate, halal certification, or organic certi-
fication, if needed, is requested from the ingredient or
flavor supplier, who obtains it through third‐party certi-
fying agencies.
Without question, selection of the right flavor enhances
desirability, prevents manufacturing and stability issues, pro-
FIGURE 12.15 Flavor descriptors and references
for cheddar cheese.
Descriptor Definition Reference
Milk fat/
lactones
Aromatics associated with
milk fat
Delta
decalactone
Fruity Aromatics associated with
different fruits
Ethyl hexanoate
Free fatty
acid
Aromatics associated with
short chain acids
Butyric acid
Phenolic Aromatics associated with
barns or animal sweat
p‐Cresol
Blue
cheese
Aromatics associated with
blue‐veined cheese
2‐Octanone
Oxidized Aromatics associated
with oxidized fat, rancidity
2,4‐Decadienal
Waxy/
crayon
Aromatics associated with
medium-chain fatty acids
Decanoic acid
or lauric acid
Nutty Nut‐like aroma associated
with roasted nuts
2,6‐Dimethyl
pyrazine
Source: M. A. Drake et al. (2001).68
vides an easy vehicle to develop product line extensions, and
improves the probability of success in the marketplace.
Flavor Enhancers
Commercial flavor enhancers are compounds that enhance
the savory taste of foods specifically by activating the umami
taste receptors in the mouth and tongue. These flavor enhanc-
ers are largely based on amino acids—the building blocks
of proteins (particularly glutamic acid)—and 5′ (5‐prime)
nucleotides—the building blocks of nucleic acids (specifically
inosine monophosphate [IMP] and guanosine monophos-
phate [GMP]). The major flavor enhancers follow.
Monosodium Glutamate (MSG)
MSG is the sodium salt of glutamic acid, one of the most
abundant naturally occurring nonessential amino acids.
Food manufacturers use MSG as a flavor enhancer because it
balances, blends, and rounds the total perception of other
tastes. It is classified by the FDA as generally recognized as
safe (GRAS). Currently, most MSG is produced by bacterial
fermentation.
Pure MSG does not have a pleasant taste by itself; how-
ever, when added to foods at very low levels MSG enhances
other taste compounds in the food, boosting the overall fla-
vor impact. MSG mixes well with meat, fish, poultry, many
vegetables, sauces, soups, and marinades, and increases the
overall preference of many foods. But more is not better : an
excess of MSG quickly ruins the taste of a dish. Typical
starting use levels for most foods are in the 0.05 to
0.20 percent range; slightly higher in very fatty or oily foods
(mayonnaise) and in topical seasonings for snack foods
(chips and so on).
Over the years, MSG has developed a negative reputa-
tion by the public as the causative agent of various aller-
genic reactions in sensitive individuals, perhaps most
infamously “Chinese Restaurant Syndrome,” which is char-
acterized by headache and numbness in the back of the
neck, sometimes extending to the shoulders and arms. It
was thought that the syndrome was caused by ingesting
foods laced with MSG, and the most common source was
Chinese food, as MSG has been used in Asian cooking ever
since it was identified as a flavor enhancer and was com-
mercially extracted from seaweed (in Japan) in the early
1900s.
In spite of the fact that there has never been a proven link
between the consumption of MSG and human illness, when-
ever monosodium glutamate is added to a food, it must be
identified as “monosodium glutamate” in the label’s ingredi-
ent list. That said, the FDA does not require foods and ingre-
dients that naturally contain glutamate to list it on the label.
Examples include tomatoes and mushrooms (particularly
dried forms in which the glutamates become concentrated),
aged cheeses, meats, soy sauce, hydrolyzed protein products
such as HVP, and autolyzed yeast extracts (AYE). These
ingredients are to be declared on the label only by their com-
mon or usual names.
c12.indd 235 1/14/2016 7:33:02 AM
236 • CULINOLOGY®
Inosinate and Guanylate (I+G)
In the flavor industry, disodium inosinate (IMP) and diso-
dium guanylate (GMP) are commonly known as disodium
ribotides, or I+G for short. They are typically sold together in
a 50:50 mixture. I+G acts synergistically with glutamates to
impart the umami flavor to foods; a mixture of 98 percent
monosodium glutamate and 2 percent I+G has four times
the flavor-enhancing power of monosodium glutamate
(MSG) alone. As a relatively expensive product, I+G is usually
not used independently of glutamates; if it is present on a
food label’s ingredient list but MSG does not appear to be, it
is possible that glutamates are provided as part of another
ingredient such as AYE or HVP, or are naturally occurring in
other ingredients such as tomatoes, Parmesan cheese, or
mushrooms.
Guanylates and inosinates are generally produced through
a fermentation process from plant raw materials such as tapi-
oca starch. They are thus suitable for vegans and vegetarians.
Autolyzed Yeast Extract (AYE)
Autolyzed yeast extract (AYE) results from the breakdown of
yeast cells. Baker’s or brewer’s yeast goes through a series of
steps to break it down and release its contents. First, salt or
mild heat is applied, causing the cell walls to lose integrity
but maintain enzyme activity. Through autolysis, the yeast’s
own enzymes break apart the proteins in the cytoplasm into
constituent amino acids, now referred to as free amino acids.
These are the source of glutamic acid and glutamates, the
same flavor enhancers found in MSG. Next, the cell wall
is ruptured to spill out the contents, and then it and other
insoluble components are removed via centrifugation, fol-
lowed by concentration and pasteurization of what remains.
This is referred to as “autolyzed yeast extract.” The final prod-
uct is available in liquid or paste form or is spray‐dried to
a powder.
Autolyzed yeast extract is used primarily as a flavor
enhancer in processed foods such as soups, meats, and vege-
tarian meat analogs. Like MSG, it is valued for its ability to
stimulate taste receptors that are sensitive to the umami or
savory type of taste, but it has a cleaner appearance on ingre-
dient labels, where it is listed as “yeast extract” without any
reference to MSG or glutamates.
It is interesting to note that while we in the United States
consider AYE strictly in its role as a flavor enhancer to be
used as a minor ingredient in other foods, other countries
consume large quantities of AYE as a food in and of itself.
Most famously, Marmite, a paste that is virtually pure AYE,
is extremely popular throughout the countries of the UK,
Australia, and New Zealand, where it has been eaten as a
spread on toast, in sandwiches, and as an accompaniment
to cheese for more than 100 years. Annual production
exceeds 6000 tons per year, with 90 percent consumed in
the UK. Starbucks in the UK even has a cheese and Marmite
panini on its menu. It is made from spent yeast from the
brewing industry, and it is an especially rich vegetarian
source of B vitamins.
Hydrolyzed Vegetable Protein (HVP)
Hydrolyzed vegetable protein (HVP) is produced by acid
hydrolysis, which involves boiling cereals or legumes, such as
soy, corn, or wheat, in hydrochloric acid and then neutraliz-
ing the solution with sodium hydroxide. The acid hydrolyzes,
or breaks down, the protein in vegetables into their compo-
nent amino acids. The resulting dark‐colored liquid contains,
among other amino acids, glutamic acid. HVP is a flavor
enhancer used in a wide variety of processed food products,
including soups, sauces, chili, stews, hot dogs, gravies, sea-
soned snack foods, dips, and dressings. It is often blended
with other spices to make seasonings that are used in or on
foods. Like AYE, it gives the product developer a way to pro-
vide glutamate enhancement without using MSG.
Acids
Acids are substances that release hydrogen ions when dis-
solved in water, giving the solution a pH below 7.0.69 They are
classified as organic acids when they include organic (car-
bon‐containing) compounds typically found in carboxylic
groups. Examples include lactic, citric, propionic, acetic, and
malic acids. Inorganic acids have no organic (carbon‐
containing) compounds and include mineral acids such as
hydrochloric acid, phosphoric acid, nitric acid, and sulfuric
acid. Acids or acidulants are used in foods for a variety of rea-
sons: to enhance flavor, to act as antimicrobial preservatives,
to prevent oxidation or discoloration of foods (addition of
ascorbic acid and citric acid to fruits), and to provide ade-
quate pH for protein functionality in food products (such as
thickening in yogurt and sour cream brought about by the
action of lactic acid, and gelation, in the case of jams and
jellies, where the pectin requires a narrow, acidic pH range
to act as a gelling agent).70 Acids also function as chelating
agents or sequestrants, which are agents that react with
metallic ions, forming complexes and thereby limiting their
catalytic properties.71 This important ability limits the rate of
lipid oxidation reactions in fats and oils by hindering the
metal ion’s ability to catalyze them.
Acid selection depends strongly on desired functionality
and on flavor. Citric and tartaric acids provide a sharp acid
profile, whereas lactic acid has a much milder sour taste with
a longer duration.
Acetic acid is an organic acid produced by microbial fer-
mentation of ethanol by acetic acid bacteria. It can also be
produced synthetically. It is the main component in vinegar,
and it is used primarily as a preservative in pickled foods and
as a flavoring agent (CFR 21 184.1005). Typical concentra-
tions of acetic acid in vinegar range from 4 to 5 percent, which
can also be expressed as the grain, which is equal to 10 times
the acetic acid content (for example, 4 percent is the same as
40 grain). It is available as a liquid of assorted grain‐strengths
or as a powder in the following forms: sodium acetate,
calcium acetate, sodium diacetate, and calcium diacetate. Its
primary applications are in pickled foods, dressings, catsup,
topical snack seasonings (in its dry form), in processed cheese
as an acidulant at 0.8 percent (CFR 21 184.1005), and as a
c12.indd 236 1/14/2016 7:33:02 AM
CHAPTER 12 • Food Additives • 237
component in baking powder, where it promotes the release
of carbon dioxide from sodium bicarbonate to provide chem-
ical leavening.
Adipic acid is an organic acid used as a flavoring agent
and acidulant in beverages and canned goods as well as
baked goods at application levels of 0.05 percent, and in jel-
lies and fruit gelatins at 0.55 percent when a prolonged sour
sensation is desired. It is also used in processed cheese and in
condiments. Adipic acid is prepared by the oxidation of nitric
acid with cyclohexanol; it is on the list of GRAS substances
(CFR 21 184.1009).
Citric acid is an organic acidulant produced either by
extraction from citrus juices such as lemon, lime, and orange
as well as from pineapple juice, or by the fungal fermentation
of carbohydrate solutions using the yeast Candida spp. or the
mold Aspergillus niger (CFR 21 184.1033). It is one of the pri-
mary acids of choice in food processing due to its strength: A
1 percent solution has a pH of 2.3 at 78°F (25°C) due to the
presence of three carboxylic acid groups in its molecule.73
Citric acid has a wide range of applications in beverages
and juices (typically used at 0.25 to 0.5 percent), confection-
ery items, and jams and jellies. It is used in processed cheese
to control pH and melt characteristics by controlling the
ratio of bound calcium to casein proteins. The lower the pH,
the more calcium leaves the protein matrix, thereby making
the cheese more flowing. It is also used as an antioxidant in
fruit and vegetable processing (apple, potato), where it
prevents enzymatic browning. It can also prevent non‐
en zymatic browning in fruit dehydration processes as an
alternative to sulfur dioxide, which produces allergic reac-
tions in sensitive individuals. Citric acid has chelating prop-
erties and can be used as an antioxidant in oils, where it
helps extend shelf life by trapping metal ions. It is also
widely used to provide or enhance the flavor of lemon and
lime in beverages, seasonings, and fruit products. Citric acid
crystals are commonly used in commercial lemon pepper
seasoning.
Fumaric acid is a strong organic acid used as an acidu-
lant in dry mix applications due to its low hygroscopicity,
which allows them to stay free‐flowing and prevents cak-
ing. It is used in fruit gelatins and jellies as an acidulant
that complements fruit flavors, and it helps pectin form
a gel.75 It can also be used in seasoning applications to
provide a low‐intensity and long‐lasting tart profile that
contrasts with the high‐intensity/short‐duration flavor of
citric acid. It is used in the shelf‐life extension of oils, acting
synergistically with other antioxidants. The ferrous fuma-
rate form is also used as a delivery system for iron in die-
tary foods (CFR 21 172.350).
Glucono‐delta‐lactone (GDL), found in honey, fruit juices,
and wine, is naturally present in its lactone or ring form,
where the carboxylic group of GDL is linked to one of its alco-
hol groups via a cyclical ester.76 The ester link breaks on addi-
tion to a liquid and slowly hydrolyzes into its gluconic acid
(an organic acid) form at a rate dependent on temperature.
This unique characteristic of GDL allows it to produce a
more gradual reduction in pH compared to other acids,
which makes it ideal for applications where slow acidifica-
tion is desired and also where high localized acid concentra-
tions are not desirable, such as directly acidified milks, dairy
products, and baked goods.77 It is used in baking powders,
where a slow release of carbon dioxide is important to control
expansion and texture. It is also used in cured and pickled
FIGURE 12.16 Properties of some acids, arranged in order of decreasing acid taste and with tartaric
acid as reference.
Acid Taste Total Acid (gm/l) pH Taste Sensation Found in
Hydrochloric +1.43 1.85 1.70 —
Tartaric 0.0 3.75 2.45 Hard Grape
Malic –0.43 3.35 2.65 Green Apple, pear, prune, grape, cherry, apricot
Phosphoric –1.14 1.65 2.25 Intense Orange, grapefruit
Acetic –1.14 3.00 2.95 Vinegar
Lactic –1.14 4.5 2.60 Sour, tart
Citric –1.28 3.50 2.60 Fresh Berries, citrus, pineapple
Propionic –1.85 3.70 2.90 Sour, cheesy
Source: deMan (1990).72
H
H C COOH
HO C COOH
H C COOH
H
FIGURE 12.17 Chemical structure of citric acid.
Source: Fennema (2007).74
c12.indd 237 1/14/2016 7:33:02 AM
238 • CULINOLOGY®
foods and dressings and can act as a metal ion sequestrant
(CFR 21 184.1318).
Lactic acid is the organic acid normally associated with
milk. It is produced microbially by lactic fermentation of sim-
ple carbohydrates, and it is present in all fermented dairy and
pickled products—yogurt, buttermilk, sauerkraut, cucumber
pickles, and so on (CFR 21 184.1061). As an ingredient, it is
available as a dry powder and also in solutions of concentra-
tions ranging from 50 to 88 percent. Lactic acid is used as an
acidulant to control pH and as a flavor agent and preserva-
tive in seasonings, olives, processed cheese, and certain acidi-
fied meat and dairy products, such as salami and cheese,
where direct acidification is used instead of fermentation
to reduce cost and speed the process of pH reduction. It is
also used in topical seasonings, dressings, and sauces when
enhancing a dairy flavor, such as ranch or buttermilk, is
desired.78
Malic acid is the main organic acid found in apples. It is
produced from the hydrogenation of fumaric acid (CFR 21
184.1069). It is tarter than citric acid at the same concentra-
tion, and it is slightly less soluble. Malic acid sustains its tart
flavor longer than other acids, and it is compatible with prod-
ucts where a fruit flavor is desired. It is used in soft drinks,
confectionery products, chewing gum, and fruit fillings. It is
preferred to citric acid in the manufacture of hard candies
because of its ease of incorporation due to its lower melting
point.79
Phosphoric acid is one of the only inorganic acids used in
foods. It is produced by burning phosphorus in air, produc-
ing phosphorus pentoxide, which yields orthophosphoric
acid when dissolved in water. Phosphoric acid is used in cola
manufacture to provide tangy flavor and the desired pH.80 It
is also used in the production of processed cheese, where it
controls melt profile by chelating ionic calcium and thus con-
trolling the amount of calcium bound to casein. Flow is hin-
dered when calcium ions remain bound to casein, and flow
increases when the calcium is chelated.81 Phosphoric acid is
also used to control the pH in food processes and as an anti-
oxidant in vegetable oils.
Tartaric acid, an organic acid, is the main acidulant
present in grapes. It has a higher tartness value than citric
acid at the same concentration, and it is used in jellies and
candies where a grape flavor is desired.82 Tartaric acid (or
cream of tartar) is also used in baking powder to release car-
bon dioxide from sodium bicarbonate, as a stabilizing agent
in meringues, and to enhance creamy texture in icings by
inhibiting crystal formation.
Succinic acid is produced by hydrogenating fumaric or
malic acid (CFR 21 184.1091). Its main use is in bread dough
to control the elasticity of gluten proteins by increasing the
number of positively charged amino acids in the gluten chain.
This increases repulsion and thereby weakens the gluten net-
work and softens the resulting baked product.83 It is also used
as an acidulant in beverages and in meat products such as
salami and cured hams. Succinic acid is unique from a flavor
perspective in that it is one of the few non‐glutamate flavor
enhancers when used at a subliminal level.
Sweeteners
Sweeteners include any substances that elicit a sweet taste
and are used in food products for that purpose. The FDA clas-
sifies sweeteners into two primary categories: nutritive and
non‐nutritive. Nutritive sweeteners include sugars, of which
the most common are glucose, fructose, and sucrose, and
polyols (sugar alcohols), which are produced by the hydro-
genation of simple carbohydrates. Their general characteris-
tic is that they have more than 2 percent of the caloric value
of sucrose per unit sweetness (CFR 21 170.3).
Non‐nutritive sweeteners are defined as substances hav-
ing less than 2 percent of the caloric value of sucrose per unit
sweetness. These include all the high‐intensity sweeteners
that are many times sweeter than sucrose and therefore have
a negligible caloric contribution because their application
level is very low (CFR 21 170.3).
Non‐Nutritive, High‐Intensity Sweeteners
Aspartame is a dipeptide consisting of two amino acids: aspar-
tic acid and phenylalanine. Aspartame has been approved in
the United States since 1981 (CFR 21 172.804). It does have a
caloric contribution when the peptide is digested, but because
it is 180 to 200 times sweeter than sucrose and is used at such
low levels, this is negligible.84 Typical usage levels are 0.01 to
0.02 percent in beverages and diet sodas. It is unstable in acidic
pH conditions, and in sodas it slowly degrades, resulting in a
decrease in sweetness over time. In addition, it is not heat sta-
ble and breaks down during cooking or baking. At retail, it is
sold under the brand names NutraSweet® and Equal® in blue
packets. The NutraSweet Company claims that aspartame is
used in more than 5000 products and consumed by some
250 million people worldwide.
Phenylketonuria (PKU) is a genetic error of metabolism
that affects about one in every 15,000 births. Individuals with
this condition cannot digest phenylalanine, one of the amino
acids in aspartame. All PKU patients must adhere to a special
diet low in phenylalanine. This requires severely restricting or
eliminating foods high in phenylalanine, such as meat, poul-
try, fish, cheese, legumes, nuts, and all foods containing
aspartame. Because of this, all foods and beverages contain-
ing aspartame must carry a warning label.
Acesulfame‐potassium (Ace‐K) has been approved in the
United States since 1988 (CFR 21 172.800). It is 200 times
sweeter than sucrose, with a metallic and bitter aftertaste. It
does not have a caloric contribution because it cannot be
metabolized by the body. Ace‐K is usually blended with other
sweeteners (sucralose or aspartame); these blends supposedly
provide a more sugar‐like taste whereby each sweetener masks
the other’s aftertaste or exhibits a synergistic effect that makes
the blend sweeter than its components. It is used in conjunc-
tion with these other sweeteners in diet sodas and desserts.
Due to its heat stability, it can also be used in bakery products.
Cyclamate was banned for use in foods in the United States
in 1969, when it was suspected of causing bladder cancer in
rats (CFR 21 189.135). Before then it was blended with saccha-
rin 10:1 to help mask saccharin’s bitter aftertaste. Cyclamate is
c12.indd 238 1/14/2016 7:33:03 AM
CHAPTER 12 • Food Additives • 239
approved for food use in 55 other countries, including the
United Kingdom and all of Europe, as well as in Canada (where
saccharin is banned). Its retail trade name is Sucaryl®.
Cyclamate is 30 times sweeter than sucrose. It has a relatively
slow onset but a more natural sugar‐like taste and the ability to
withstand typical cooking and baking temperatures.
Neotame is a high‐intensity sweetener with a short peptide‐
chain backbone; it is 7000 to 13,000 times sweeter than sucrose.
It was created by the same company that owns aspartame:
Monsanto. It is approved for use in foods, except for meat and
poultry (CFR 21 172.829). Even though only trace amounts of
neotame are needed to sweeten foods due to its potency, it still
exhibits the characteristic bitter aftertaste common to all non‐
nutritive sweeteners. While it is approved for use in 60 coun-
tries, including the United Kingdom, the European Union, and
Canada, and is ranked “safe” by the Center for Science in the
Public Interest, it is not yet available to consumers.
Saccharin is the oldest commercially manufactured syn-
thetic sweetener in existence. It was originally manufactured by
the Monsanto Company for the Coca-Cola Company in 1921. It
is 300 to 400 times sweeter than sucrose.85 Due to its low thermal
stability, it is used in low‐calorie beverages and products that do
not undergo a severe thermal process. Saccharin is often used
with aspartame in diet soda fountain syrups so that some sweet-
ness remains should the fountain syrup be stored beyond aspar-
tame’s relatively short shelf life. It is recognizable at retail as the
pink packet with the trade name Sweet ‘n’ Low®.
Sucralose is produced by the chlorination of the sucrose
molecule. It is 600 to 800 times sweeter than sucrose and has a
similar flavor profile, with a slight to moderate metallic after-
taste. It is stable at high temperatures, which makes it suitable
for bakery and confectionery products. It is also stable under
acidic conditions, so it can be used in diet soft drinks.86 It is sold
at retail under the brand name Splenda® in yellow packets. The
commercial success of sucralose stems from its favorable com-
parison to other low‐calorie sweeteners in terms of taste, sta-
bility, and safety. It is one of only two artificial sweeteners
(neotame is the other) to receive a “safe” ranking from the
Center for Science in the Public Interest. Sucralose can be
found in more than 4500 food and beverage products, includ-
ing candy, breakfast bars, and soft drinks. It is also used in
canned fruits in place of higher‐calorie corn syrups.
Steviosides (stevia) are sweet glycosides extracted from the
leaves of the Stevia rebaudiana plant. Stevia is 300 times
sweeter than sucrose and is heat stable, pH stable, and non‐
fermentable. The sweetest fractions, stevioside and rebaudio-
side A, have recently been commercialized in the United
States. After many years of petitioning the FDA, stevia was
finally granted GRAS status in 2008. It is currently the only
natural high‐intensity sweetener in widespread use. It is avail-
able at retail under two competing brand names: Truvia®
( jointly owned by Cargill and Coca‐Cola) and PureVia® ( jointly
owned by PepsiCo and Merisant). Unfortunately, it shares the
same chemical aftertaste as all of the synthetics.
Luo han guo (Siraitia grosvenorii), also known as monkfruit,
is an herbaceous perennial vine native to southern China
and northern Thailand. The plant is best known for its fruit.
FIGURE 12.18 Relative sweetness of nutritive
sweeteners.
Substance
Relative Sweetness Value (compared
to sucrose = 100 at equivalent weight)
Glucose
(Dextrose)
70
Fructose 150
Invert sugar 130
HFCS 42
HFCS 55
90
95
Erythritol 60–70
Isomalt 50–60
Lactitol 30–40
Maltitol 90
Mannitol 50
Sorbitol 60
Xylitol 100
Source: Igoe and Hui (2001).87
The fruit extract is nearly 300 times sweeter than sugar and has
been used as a natural low‐calorie sweetener in China for
nearly a millennium to treat diabetes and obesity. The sweet
taste of the fruit comes mainly from mogrosides, a group of
triterpene glycosides that make up approximately 1 percent of
the flesh of the fresh fruit. Through solvent extraction, a pow-
der containing 80 percent mogrosides can be obtained, the
main one being mogroside‐5. The process for the manufacture
of a useful sweetener from luo han guo was patented in 1995 by
Procter & Gamble, the main objective of which is to remove an
assortment of interfering aromas from the fruit that otherwise
render it useless for widespread use. Since luo han go received
GRAS status in 2007, several companies have launched new
products containing it, including low‐calorie juices, herbal
teas, protein bars, and breakfast cereals.
Thaumatin is a natural, low‐calorie (virtually calorie‐free)
protein‐based sweetener and flavor modifier. The thaumatins
were first found as a mixture of proteins isolated from the
katemfe fruit (Thaumatococcus daniellii Bennett) of West Africa.
This “miracle fruit” is more widely known for its ability to make
sour substances (citrus juices, vinegar, and so on) taste sweet
after one chews its pulp. Some of the proteins in the thaumatin
family are natural sweeteners, roughly 2000 times more potent
than sugar. Although very sweet, thaumatin’s taste is markedly
different from that of sugar. The sweetness of thaumatin builds
slowly, and the sweetness perception lasts a long time, leaving a
licorice‐like aftertaste at high usage levels. Thaumatin is highly
water soluble and stable to heating and acidic conditions. In
the United States, it is considered to be a GRAS flavoring agent.
The extract is available as an industrial ingredient under the
tradename Talin®, and it is marketed more as a flavor modifier
and bitterness masker than a sweetener.
c12.indd 239 1/14/2016 7:33:03 AM
240 • CULINOLOGY®
Sodium Reduction
Reducing sodium in the diet (primarily due to the consump-
tion of table salt, sodium chloride) is a goal of many govern-
ments and manufacturers, and it is quickly becoming a
priority in the United States on par with reducing and elimi-
nating trans fats from the diet. Strict government guidelines
are already in effect in the United Kingdom, and national ini-
tiatives are underway in Canada, Australia, Finland, France,
Ireland, and New Zealand to reduce sodium in food. In
January 2010, New York City announced its partnership with
various organizations through the National Salt Reduction
Initiative (NSRI), the goal of which is to cut the sodium in
packaged and restaurant foods by 25 percent over five years.
At the time NSRI was launched, it was a voluntary initiative,
but it is thought that the amount of sodium in food could be
federally regulated at some point. The NSRI is focusing on
reducing sodium in packaged and restaurant food because it
is believed that only 5 to 11 percent of the sodium in a typical
American’s diet comes from putting additional salt on food at
the table.89 A gradual step‐down approach is believed by most
the best way to achieve the goal of reducing sodium in food; a
sudden reduction of 25 percent could lead to adverse con-
sumer reaction because of the resulting radical change in fla-
vor of most products.
Reducing sodium is a challenging prospect for any
product developer, as no single ingredient works in all
applications to replace the flavor and functionality of
sodium chloride. The role of sodium chloride in foods
includes not only taste but also protein solubilization, as
in the case of meat products to enhance water‐holding
capacity ; as a contributor to dough strength in bakery
items; and as an antimicrobial agent. Furthermore, due to
the low cost of sodium chloride, it is extremely difficult to
find cost‐neutral alternatives. The first step for the prod-
uct developer is to evaluate the formula or product as a
whole to determine the role salt plays in the food system.
FIGURE 12.19 Relative sweetness of
high‐intensity sweeteners.
Substance
Relative Sweetness Value (compared
to sucrose = 1 at equivalent weight)
Aspartame 180–200
Acesulfame‐K 200
Cyclamate 30
Neotame 7000–13,000
Saccharine 300–400
Steviosode 300
Sucralose 600–800
Thaumatin 1600–2000
Source: Igoe and Hui (2001).88
Once that determination is made, strategies for salt reduc-
tion can be better formulated.
In terms of maintaining salty flavor, there are three areas
of focus for sodium‐reduction efforts in processed foods,
each with its own limitations: salt substitutes; modifications
to the physical makeup of the salt, and flavor‐enhancing
ingredients.90
The only other known substance that elicits a salty taste
that is approved for human consumption is potassium chlo-
ride. Its saltiness intensity is less pronounced than that of
sodium chloride, and it delivers a metallic and bitter after-
taste that is detected by a large portion of the population.91
Therefore, a product developer is likely to use potassium
chloride as a partial replacer for salt. Potassium chloride’s
effectiveness depends on whether it is dissolved, as in a soup
or sauce, or added topically, as on a pretzel.
In food products where it is used in solution (soups,
sauces, brines, and so on), potassium chloride can work well
as a salt substitute in most applications, substituting up to
25 percent of the salt used.92 The undesirable flavors of potas-
sium chloride become evident at higher substitution levels
but can be mitigated somewhat by bitterness‐masking
agents. Most flavor companies offer products that are a blend
of potassium chloride and proprietary bitterness blockers.
One of the drawbacks of supplementing products with potas-
sium ions is the risk of producing an adverse effect on seg-
ments of the populations that are at risk for kidney problems
or heart failure due to potassium sensitivity.93
Various companies have successfully used other sources
of salt to reduce sodium in food products. Sea salt is naturally
lower in sodium than table salt because so many non‐sodium
mineral salts, such as potassium, magnesium, and calcium,
are dissolved in the ocean. The flavor these extra minerals
impart has bitter and metallic notes similar to that of potas-
sium chloride. In addition, the main component of sea salt is
still NaCl, so the actual amount of sodium reduction depends
on the ratio of the non‐sodium salts to NaCl in the blend.
Each sea salt is different and must be analyzed for sodium
before using.
Another strategy is using salt with altered crystalline
structure. Salts with larger crystals, or those that are irregu-
larly shaped, with porous structures and increased surface
area, can be created by manipulating the salt‐drying process.
These allow greater salty taste with smaller amounts of salt;
this is particularly useful in applications where salt is used on
the surface of food products. Replacing table salt 1:1 (volume
to volume, NOT weight to weight) with these larger salts does
reduce sodium in formulas, but whether the total saving is
significant enough to make a big difference in sodium con-
sumption remains to be seen.
The use of flavor enhancers (MSG, AYE, I+G, HVP) can
enhance perceived saltiness. In a reduced‐salt application,
the enhancer increases the perception of what little salt is
present and makes it taste stronger. A combination of I+G
and MSG can be added to a food to reduce the salt content.
The effect of this addition is to impart a magnification of the
savory taste sensation by enhancing the food’s natural flavors
c12.indd 240 1/14/2016 7:33:03 AM
CHAPTER 12 • Food Additives • 241
FIGURE 12.20 Selected examples of proposed salt substitutes.
Substitute Applications Comments
Potassium chloride (KCl) Many foods, including cheeses,a
breads,b and meats,c may be
mixed with NaCl in up to a
50:50 ratio.c
Bitter to many people;c many patents to reduce
KCl bitterness exist;d because potassium intake
of the U.S. population is low, increased intake
of potassium may benefit somee but could
harm certain subpopulations (e.g., those with
certain medical conditions or taking certain
medications).f
Lithium chloride (LiCl) None: Toxic although almost
perfectly salty
Calcium chloride (CaCl
2
),
magnesium chloride (MgCl
2
),
and magnesium sulfate (MgSO
4
)
Few foods Somewhat salty but with many off tastes;g bitter
tastes of MgSO
4
are usually perceived only at
high levels;h CaCl
2
can cause irritations on the
tongue.h
Sea salt Many foods, also used in
salt shakers
Usually contains substantial amounts of sodium
chloride; benefits of use in reducing sodium
consumption are unclear
Salts with altered crystal
structure
Some foods Porous and star‐shaped structures, created
by manipulating the salt‐drying process, allow
greater salty taste with smaller amounts of salt;i
particularly useful in applications where salt is
used on the surface of food products.j
aGuinee and O’Kennedy, 2007.
bCauvain, 2007.
cDesmond, 2007
dPorzio, 2007.
eAnthony, 2007.
fDietary Guidelines Advisory Committee, 2005.
gMurphy et al., 1981.
hKilcast and den Ridder, 2007.
iDesmond, 2006.
jPszczola, 2007.
REFERENCES
Ainsworth, P., and A. Plunkett. 2007. Reducing salt in snack products. In Reducing salt in foods: Practical strategies, edited by D. Kilcast and
F. Angus. Cambridge, UK: Woodhead. Pp. 296‐315.
Ajinomoto Food Ingredients LLC. 2008. Ajinomoto monoammonium glutamate (MAG) and monopotassium glutamate (MPG). http://www
.ajiusafood.com/5MAG/main.asp (accessed 2008).
Anthony, M. 2007. Season with (only) a grain of salt. http://www.foodprocessing.com/articles/2007/204.html (accessed September
2008).
Armor Proteines. 2007. Lactosalt Optitaste.http://www.armor‐proteines.com/ENG/lactosalt_UK (accessed 2008).
Brandsma, I. 2006. Reducing sodium: A European perspective. Food Technology 60(3): 24–29.
Breslin, P. A. S., and G. K. Beauchamp. 1995. Suppression of bitterness by sodium: Variation among bitter taste stimuli. Chemical Senses
20(6): 609–623.
Cauvain, S. P. 2007. Reduced salt in bread and other baked products. In Reducing salt in foods: Practical strategies, edited by D. Kilcast and
F. Angus. Cambridge, UK: Woodhead. Pp. 283–295.
Desmond, E. 2006. Reducing salt: A challenge for the meat industry. Meat Science 74(1): 188–196.
Desmond, E. 2007. Reducing salt in meat and poultry products. In Reducing salt in foods: Practical strategies, edited by D. Kilcast and
F. Angus. Cambridge, UK: Woodhead. Pp. 233–255.
Dietary Guidelines Advisory Committee. 2005. Report of the Dietary Guidelines Advisory Committee on the Dietary Guidelines for
Americans, 2005. A Report to the Secretary of Health and Human Services and the Secretary of Agriculture. Washington, DC: U.S.
Department of Agriculture and U.S. Department of Health and Human Services. DSM Food Specialties. 2004. Maxarite—product
properties. http://www.dsm.com/le/en_US/maxarite/html/properties.htm (accessed December 12, 2008).
Guinee, T. P., and B. T. O’Kennedy. 2007. Reducing salt in cheese and dairy spreads. In Reducing salt in foods: Practical strategies, edited
by D. Kilcast and F. Angus. Cambridge, UK: Woodhead. Pp. 316–357.
Jungbunzlauer AG. 2007. Sub4salt: Jungbunzlauer’s way to reduce sodium. Switzerland: Jungbunzlauer AG.
Keast, R. S. J., T. M. Canty, and P. A. S. Breslin. 2004. The influence of sodium salts on binary mixtures of bitter‐tasting compounds.
Chemical Senses 29(5): 431–439.
(continued)
c12.indd 241 1/14/2016 7:33:03 AM
242 • CULINOLOGY®
Source: Reprinted with permission from Henney, J. E., C. Taylor, C. S. Boon, and The Committee on Strategies to Reduce Sodium Intake,
Strategies to Reduce Sodium Intake in the United States (Washington, DC: National Academies of Science, 2010). Courtesy of the National
Academies Press.94
Kilcast, D. 2007. Cutting sodium. Prepared Foods, January: 1–5.
Kilcast, D., and C. den Ridder. 2007. Sensory issues in reducing salt in food products. In Reducing salt in foods: Practical strategies, edited
by D. Kilcast and F. Angus. Cambridge, UK: Woodhead. Pp. 201–220.
Murphy, C., A. V. Cardello, and J. G. Brand. 1981. Tastes of fifteen halide salts following water and NaCl: Anion and cation effects.
Physiology and Behavior 26(6): 1083–1095.
Ndabikunze, B. K., and S. Lahtinen. 1989. Substitution of sodium chloride by Morton lite salt or mineral salt in mayonnaise. International
Journal of Food Science and Technology 24(4): 367–371.
Porzio, M. 2007. Flavor delivery and product development. Food Technology, January: 22–29.
Pszczola, D. 2006. Exploring new ‘tastes’ in textures. Food Technology, January: 44–55.
Pszczola, D. 2007. Savoring the possibilities. Food Technology 61(4): 55–66.
Yamaguchi, S. 1987. Fundamental properties of umami in human taste sensation. In Umami: A basic taste, edited by Y. Kawamura and M. R.
Kare. New York: Marcel Dekker.
FIGURE 12.21 Selected examples of proposed salt enhancers.
Ingredient Applications Comment
Monosodium glutamate (MSG) and
other glutamates
Many foods; can
replace some salta
No pleasant taste in itself, but enhances salty
tastes; imparts the taste of umami; MSG
contains sodium; other glutamate salts such
as monopotassium glutamate or calcium
diglutamate may further reduce sodium;
synergizes with 5′‐ribonucleotides;b may
replace bitter blockingc and oral thickeningd
characteristics; often contained in hydrolyzed
vegetable protein and yeast extracts.a
Yeast extracts and hydrolyzed
vegetable protein
Some foods Often contains MSG, but is seen as a “natural”
alternative to MSG use; meaty and brothy
tastes limit potential uses.d,e
Nucleotides, including inosine‐5′‐
monophosphate (IMP) and
guanosine‐5′‐monophosphate
Some foods Imparts the taste of umami; found to act
synergistically with glutamates to enhance salty
tastes in some foods.d,f
Amino acids, especially arginine and
related compounds
Not known L‐Arginine is reported to enhance the saltiness
of foods with low to moderate levels of salt;
practical uses are not clear.g
Dairy concentrates Many foods Reported to allow moderate sodium reductions
in a variety of products.e,h
Lactates (potassium lactate, calcium
lactate, and sodium lactate)
Few foods May enhance the saltiness of NaCl, but not
widely used; calcium lactate can impart a sour
taste.b
Herbs and spices Many foods Herbs and spices provide other flavoring
characteristics and may, for some people, help
alleviate blandness following salt removal.i,j
Compounds that reduce bitterness,
including adenosine‐5′‐monophosphate,
DHB (2,4‐dihydroxybenzoic acid), lactose,
sodium gluconate, and mixtures for use in
combination with potassium chloride
Many foods Designed to mask bitterness of potassium
chloride or reduce bitterness from other
food components that are usually masked by
salt; allow partial reduction of total sodium
content.b,e,k,l
Mixtures of NaCl substitutes and
enhancers
Many foods Proprietary mixtures are produced by many
companies; mixtures consist of a number of
ingredients such as non‐sodium salts, yeast
extracts, potassium chloride, sodium, and
sodium gluconate.m,n,o
FIGURE 12.20 (continued)
(continued)
c12.indd 242 1/14/2016 7:33:03 AM
CHAPTER 12 • Food Additives • 243
as well as any added salt. Even though it appears you are
replacing sodium (in salt) with sodium (in MSG), the sodium
content of MSG is roughly 3 times lower (12 percent) than in
sodium chloride (39 percent).
Certain acids can also be used to enhance flavors similarly
to salt. Recent studies have shown that black rice vinegar,
with its higher amino acid level, had a pronounced effect on
enhancing the perception of saltiness.95
Conclusion
Food additives are employed for multiple purposes, including
flavor and texture improvement, shelf life extension, antimi-
crobial and antioxidant activity, color preservation, and
increasing the nutritional value of food products. They are
added to help maintain and enhance the natural quality
attributes of foods through the multiple, sometimes deleteri-
ous changes brought about by processing. Most processed
food products contain additives to some degree and in vari-
ous combinations. Therefore, product developers must be
familiar with the wide range of food additives, and why and
how they are used, in order to effectively design successful,
commercially viable products.
1H. This, Molecular Gastronomy: Exploring the Science of Flavor, M. B.
Debevoise (trans.) (New York: Columbia University Press, 2006).
2N. Potter and J. Hotchkiss, Food Science, 5th ed. (New York: Chapman and
Hall, 1995).
3G. L. Christen and J. S. Smith, Food Chemistry: Principles and Applications
(West Sacramento, CA: Science Technology System, 2000).
4C. Hazen, “Antioxidants ‘Meat’ Needs,” Food Product Design (2005): 61–68.
5Christen and Smith, Food Chemistry.
6V. A. Vaclavik and E. W. Christian, Essentials of Food Science, 3rd ed. (New
York: Springer Science and Business Media, 2008).
7Ibid.
8O. R. Fennema, Food Chemistry, 4th ed. (New York: Marcel Dekker, 2007).
9D. Marmion, Handbook of U.S. Colorants: Foods, Drugs, Cosmetics, and
Medical Devices, 3rd ed. (New York: John Wiley & Sons, 1991).
10M. Ash and I. Ash, Handbook of Food Additives, 2nd ed. (Endicott, NY:
Sinapse Information Resources, 2002).
11Fennema, Food Chemistry.
12Code of Federal Regulations, Title 21 (CFR 21 73.95), retrieved from www
.fda.gov ; J. M. de Man, Principles of Food Chemistry, 2nd ed. (New York:
Van Nostrand Reinhold, 1990).
13Marmion, Handbook of U.S. Colorants.
14J. Smith, Food Additive User’s Handbook (New York: Van Nostrand Reinhold,
1991).
15R. S. Igoe and Y. H. Hui, Dictionary of Food Ingredients (Gaithersburg, MD:
Aspen Publishers, 2001).
16Fennema, Food Chemistry.
17Smith, Food Additive User’s Handbook.
18Igoe and Hui, Dictionary of Food Ingredients.
19Fennema, Food Chemistry, 4th ed.
20F. J. Francis, Colorants (St. Paul, MN: American Association of Cereal
Chemists, 1999).
21https://cspinet.org/new/pdf/food‐dyes‐rainbow‐of‐risks .
22Marmion, Handbook of U.S. Colorants.
23Smith, Food Additive User’s Handbook.
24Fennema, Food Chemistry.
25Marmion, Handbook of U.S. Colorants.
26Ibid.
27Fennema, Food Chemistry.
28Francis, Colorants; Code of Federal Regulations, Title 21 (CFR 21 74.706),
retrieved from www.fda.gov.
29Igoe and Hui, Dictionary of Food Ingredients.
30Fennema, Food Chemistry, 4th ed.
31Francis, Colorants.
32Ibid.
33de Man, Principles of Food Chemistry.
34Fennema, Food Chemistry.
35Y. Pomeranz, Functional Properties of Food Components (Orlando, FL:
Academic Press, Inc., 1985).
36Ash and Ash, Handbook of Food Additives.
37Igoe and Hui, Dictionary of Food Ingredients.
38Smith, Food Additive User’s Handbook.
39H. McGee, On Food and Cooking: The Science and Lore of the Kitchen (New
York: Scribner, 2004).
FIGURE 12.21 (continued)
Source: Reprinted with permission from Henney, J. E., C. Taylor, C. S. Boon, and The Committee on Strategies to Reduce Sodium Intake,
Strategies to Reduce Sodium Intake in the United States (Washington, DC: National Academies of Science, 2010). Courtesy of the National
Academies Press.96
aYamaguchi, 1987.
bKilcast and den Ridder, 2007.
cKeast et al., 2004.
dBrandsma, 2006.
ePszczola, 2007.
fAjinomoto Food Ingredients LLC, 2008.
gBreslin and Beauchamp, 1995.
hArmor Proteines, 2007.
iKilcast, 2007.
jAinsworth and Plunkett, 2007.
kNdabikunze and Lahtinen, 1989.
lDesmond, 2007.
mDSM Food Specialties, 2004.
nPszczola, 2006.
oJungbunzlauer, 2007.
See the references in Figure 12.20.
c12.indd 243 1/14/2016 7:33:03 AM
http://www.fda.gov
244 • CULINOLOGY®
40Fennema, Food Chemistry.
41Igoe and Hui, Dictionary of Food Ingredients.
42Ibid.
43Smith, Food Additive User’s Handbook.
44Fennema, Food Chemistry.
45L. A. Kuntz, “Figuring Out Phosphates” ( June 2006), retrieved from http://
www.foodproductdesign.com/articles/2006/06/figuring‐out‐phosphates
.aspx.
46Christen and Smith, Food Chemistry.
47D. J. Thomas and W. A. Atwell, Starches (St. Paul, MN: American Association
of Cereal Chemists, 1999).
48Phosphate Forum of America. “Where Might You Encounter Phosphates
Today” (2010), retrieved April 22, 2010, from http://www.phosphatesfacts
.org/uses_apps.asp
49Kuntz, “Figuring Out Phosphates.”
50Fennema, Food Chemistry.
51Ash and Ash, Handbook of Food Additives.
52Igoe and Hui, Dictionary of Food Ingredients.
53Y. H. Hui, Encyclopedia of Food Science and Technology (New York: John
Wiley & Sons, 1992).
54Fennema, Food Chemistry.
55Ibid.
56Igoe and Hui, Dictionary of Food Ingredients.
57Fennema, Food Chemistry, 4th ed.
58Ibid.
59Igoe and Hui, Dictionary of Food Ingredients.
60R. L. Hall, “Flavor and Flavoring: Seeking a Consensus of Definition,” Food
Technology 22 (1968): 1496.
61Journal of the Chemical Society of Tokyo, No. 30, 820–836 (1909).
62USDA Foreign Agricultural Service, GAIN Report Number: GR7020, 1/14/2008.
63Code of Federal Regulations, Title 21, “Foods: Labeling of Spices, Flavorings,
Colorings, and Chemical Preservatives,” 21 C.F.R. § 202.1 Section 3 (2009),
retrieved from www.fda.gov.
64USDA Food Standards and Labeling Policy Book, August 2005.
65G. A. Reineccius, “Flavor Encapsulation,” Food Reviews International 5, no. 2
(1989): 147.
66G. S. Sinki and R. J. Gordon, “Flavoring Agents,” in Food Additives, A. L.
Brannen et al., eds. (New York: Marcel Dekker, 1992).
67M. A. Drake et al., “Development of a Descriptive Language for Cheddar
Cheese,” Journal of Food Science 66, no. 9 (2001): 1422.
68Ibid.
69R. S. Shallenberger, Taste Chemistry (Cambridge, UK: Blackie Academic and
Professional, 1992).
70Igoe and Hui, Dictionary of Food Ingredients.
71Fennema, Food Chemistry.
72de Man, Principles of Food Chemistry, 2nd ed.
73Igoe and Hui, Dictionary of Food Ingredients.
74Fennema, Food Chemistry, 4th ed.
75Ash and Ash, Handbook of Food Additives.
76Fennema, Food Chemistry.
77Igoe and Hui, Dictionary of Food Ingredients.
78Ash and Ash, Handbook of Food Additives.
79Igoe and Hui, Dictionary of Food Ingredients.
80Ibid.
81Ash and Ash, Handbook of Food Additives.
82Igoe and Hui, Dictionary of Food Ingredients.
83McGee, On Food and Cooking.
84Fennema, Food Chemistry.
85Igoe and Hui, Dictionary of Food Ingredients.
86Ibid.
87Ibid.
88Ibid.
89New York City Department of Health and Mental Hygiene, “Cutting Salt,
Improving Health” (2010), retrieved from http://www.nyc.gov/html/doh
/html/cardio/cardio‐salt‐initiative.shtml.
90S. Daniells, “The Science of Salt Reduction in Food” ( June 2006), retrieved
from http://www.foodnavigator‐usa.com/content/view/print/142798.
91Trading Standards Institute, “The Salt Reduction Toolkit” (2008), retrieved
from http://www.tradingstandards.gov.uk/policy/policy‐saltreductiontoolkit
.cfm.
92J. E. Henney, C. Taylor, C. S. Boon, and The Committee on Strategies to
Reduce Sodium Intake, Institute of Medicine, “Strategies to Reduce
Sodium Intake in the United States” (Washington, DC: National Academies
Press, April 2010), retrieved from Institute of Medicine website: http://
www.iom.edu/Reports/2010/Strategies‐to‐Reduce‐Sodium‐Intake‐in‐the‐
United‐States.aspx.
93Trading Standards Institute, “The Salt Reduction Toolkit.”
94Henney, Taylor, Boon, and The Committee on Strategies to Reduce Sodium
Intake, Institute of Medicine, “Strategies to Reduce Sodium Intake in the
United States.”
95C. Scott‐Thomas, “Vinegar Could Be Used for Salt Reduction: Study” (May
2009), retrieved from http://www.foodnavigator.com/Science‐Nutrition
/Vinegar‐could‐be‐used‐for‐salt‐reduction‐Study.
96Henney, Taylor, Boon, and The Committee on Strategies to Reduce Sodium
Intake, Institute of Medicine, “Strategies to Reduce Sodium Intake in the
United States.”
c12.indd 244 1/14/2016 7:33:03 AM
13
CHAPTER 13 • Food Safety and Spoilage • 245
Food Safety
and Spoilage
Introduction
While everyone who produces food for public consumption
intends their products to be enjoyable, sometimes they
cause unintended illness. Food obtained from land or
water may contain chemical, physical, or biological haz-
ards that cause illness or injury. It can also be contami-
nated with microorganisms that initiate spoilage (decay
and rot) as soon as the animal is killed, or when the fruit or
vegetable is harvested. There are two parts to food safety
and spoilage. The first deals with the product developer ’ s
responsibility to control the hazards in food to assure con-
sumers an appropriate level of protection (ALOP) . The sec-
ond deals with controlling contamination of food that can
lead to spoilage so consumers’ expectations of quality and
freshness are met.
The regulatory standards of both the U.S. Food and Drug
Administration (FDA) and the U.S. Department of Agriculture
(USDA), though slightly different, are used to develop food
products with similar safety results.
Food contamination begins when animals and humans
pollute the water and soil used for the production of crops
and livestock. Waterways from which food is taken may be
contaminated by fecal or chemical runoff from farms, sewage
from towns and cities, and chemical waste from processing
facilities.
On farms, animal feces may contaminate the hair,
feathers, and other surfaces of the animal. During slaugh-
ter, contamination on the surface of raw meat and poultry
can be spread throughout the carcass. Fruits and vegeta-
bles irrigated with contaminated water or fertilized with
fresh animal manure may also become hazardous. In addi-
tion, inadequate control of antibiotics or hormones fed to
meat animals and of insecticides and pesticides applied
to fruits and vegetables can lead to contamination of food
or water. 1
Most spoilage microorganisms do not cause illness. On
the other hand, foodborne illness organisms ( pathogens )
normally do not change the odor, appearance, or taste of the
food. This is what makes them so dangerous—there may be
no obvious signs of spoilage to warn the consumer: “Don ’ t
eat!” To assure safety, standardized, proven food‐handling
procedures must be followed. The principal control docu-
ment in the product development kitchen or laboratory is
the HACCP (Hazard Analysis and Critical Control Points)
recipe, which is designed to reduce hazards to the safest level
possible.
Food Safety
A recent review of epidemiologic data showed that each year
31 major pathogens acquired in the United States caused
48 million episodes of foodborne illness, 128,000 hospitaliza-
tions, and 3,000 deaths. 2 That means that 1 in 6 individuals in
the United States are affected by some form of foodborne illness
each year . Continuing outbreaks every year show this prob-
lem is not going away. In March 2010, a report by the Produce
Safety Coalition, the Make our Food Safe Coalition, and the
Pew Charitable Trust estimated the economic impact of
foodborne illness based on health‐related costs (physician
services, pharmaceuticals, and hospital costs) and losses to
quality of life (lost life expectancy, pain and suffering, and
functional disability) exceeded $150 billion annually. 3 The
cost of a foodborne illness outbreak can easily put a food
establishment out of business.
As the first step in new product development, the food
processor determines if the food will be shelf‐stable at room
temperature, refrigerated, or frozen, and then processes and
packages it accordingly. The consumer who purchases this
food is responsible for proper handling after purchase to
maintain its safety.
Lead Author: O. Peter Snyder , Jr., Ph.D. , President, SnyderHACCP
Owner, A La Carte Connections, LLC Rachel B. Zemser , MS, CCS® ,
c13.indd 245 1/22/2016 1:14:18 PM
246 • CULINOLOGY®
FIGURE 13.2 Selected foodborne illness outbreaks, United States, 2007.
Month State Etiology Location Total Ill
Total Hospital‐
izations
Total
Deaths Vehicle
June Illinois Salmonella Heidelberg
(confirmed)
Fair, festival,
temporary mobile
service
802 29 0 Hummus
July Illinois Norovirus (confirmed) Other 526 0 0
February Multistate
outbreak
reported by CDC
Salmonella I 4,[5],
12:i:‐ (confirmed)
Private home 401 108 3 Pot pie
August Washington Clostridium perfringens
(confirmed)
Prison, jail 300 0 0
December California Norovirus (confirmed) Other; restaurant or
deli; banquet facility
168 2 0
May New Jersey Clostridium perfringens
(confirmed)
Prison, jail 145 0 0 Turkey ziti,
casserole
July Colorado E. coli, Shiga‐toxin
producing O121
(confirmed); E. coli,
Shiga‐toxin producing
O26 (confirmed);
E. coli, Shiga‐toxin
producing O84
(confirmed)
Prison, jail 135 10 0 American
cheese,
pasteurized;
margarine
Home
1
2
8
6
3
4
57
Is the food
safe?
The critical
control point
Slaughter
Food process
FIGURE 13.1 Food “from farm to fork.” The flow of food from the growing area to the consumer.
Used by permission of the Hospitality Institute of Technology and Management, St. Paul, MN.
(continued)
c13.indd 246 1/14/2016 7:33:58 AM
CHAPTER 13 • Food Safety and Spoilage • 247
Month State Etiology Location Total Ill
Total Hospital‐
izations
Total
Deaths Vehicle
November California Norovirus (suspect) Wedding reception 133
January Oregon Clostridium perfringens
(suspect)
Prison, jail 132 0 0 Spaghetti,
unspecified
June Washington Norovirus (confirmed) Restaurant or deli 128 Lettuce‐
based salads
May New Jersey Norovirus (confirmed) Banquet facility 127 1 0 Chicken marsala
January California Norovirus (confirmed) Restaurant or deli;
office setting;
private home
125 1 0
September Colorado Clostridium perfringens
(confirmed)
Prison, jail 125 0 0 Beans, chili
May California E. coli, Shiga‐toxin
producing O157:H7
(confirmed)
Other 124 8 0 Tri‐tip
December Ohio Norovirus (confirmed) Restaurant or deli 119 2 0
April Virginia Salmonella Enteritidis
(confirmed)
Restaurant or deli;
banquet facility;
wedding reception
106 14 0 Chicken wrap;
steak, prime
rib; sandwich,
chicken
February Texas Norovirus (confirmed) Nursing home 89 5 2
January Multistate
outbreak
reported by CDC
Salmonella Enteritidis
(confirmed)
Private home 81 9 0 Eggs,
scrambled
July California Shigella sonnei
(confirmed)
Restaurant or deli 72 9 0 Lettuce‐
based salads
June Multistate
outbreak
reported by CDC
Salmonella Newport
(confirmed)
Restaurant or deli;
private home
65 11 0 Tomato,
beefsteak
June Alabama E. coli, Shiga‐toxin
producing O157:H7
(confirmed)
Restaurant or deli 26 11 1 Lettuce‐
based salads,
unspecified
Source: CDC (2009)4.
FIGURE 13.2 (continued)
c13.indd 247 1/14/2016 7:33:58 AM
248 • CULINOLOGY®
FIGURE 13.3 Major food pathogen hazards.
Microorganisms and Source
(Atmosphere, Temperature Range for Growth,
pH Range, and Minimal Water Activity [aw])
Illness
(Incubation Period, Symptoms, Duration)
VEGETATIVE PATHOGENIC BACTERIA
Campylobacter jejuni
Source: Poultry, raw milk, food contaminated by
infected workers, cross‐contamination
Growth: Obligate microaerophile; growth temp.
range: 86–113°F; 4.9–9.0 pH; >0.98 aw
Destruction D [137°F] = 12–21 sec.
Onset: 1 to 10 days (usually 2 to 5 days)
Symptoms: Abdominal pain, diarrhea (may contain occult blood),
headache, fever, nausea, feeling of ill health, loss of appetite,
muscle pain. Many infections are without symptoms. Chronic
consequences include reactive arthritis, Guillain‐Barré syndrome.
May mimic appendicitis or inflammatory bowel disease.
Duration: 7 to 10 days (Relapse occurs in about 25% of cases.)
Inf. Dose: ≥500 CFU
Salmonella spp.
Source: Raw meat and poultry, seafood, eggs, raw
sprouts, raw vegetables, raw milk, unpasteurized
juices, food contaminated by infected workers
via fecal/oral route.
Growth: Grows with or without air; growth temp.
range: 41.5–114°F; 4.1–9.0 pH; 0.92–0.95 aw
Destruction
D [140°F] = 2–6 min.
Onset: 6 to 72 hours (usually 12 to 36 hours)
Symptoms: Abdominal pain, diarrhea, nausea, vomiting, chills,
fever, headache, feeling of ill health, loss of appetite. Chronic
consequences include septicemia, arthritis, endocarditis,
meningitis, pericarditis, and pneumonia.
Duration: Acute symptoms may last for 1 to 2 days, or may be
prolonged, depending on host factors, ingested dose, and strain.
Inf. Dose: 1 to about 1010 CFU (depends on strain and individual;
usually 100 to 1000 organisms)
Listeria monocytogenes
Source: Raw meat and poultry, fresh soft cheese,
paté, smoked seafood, deli meats and salads.
Growth: Grows with or without air; growth temp.
range: 29.3–112°F; 4.5–9.5 pH; 0.90–0.93 aw
Destruction
D [140°F] = 2.85 min.
D [158°F] = 10 sec.
Onset: Variable: 3 to 70 days (median 3 weeks)
Symptoms: Fever, headache, nausea, vomiting, diarrhea precede
complications of stillbirths, meningitis, encephalitis, sepsis.
Use of antacids may predispose healthy people to illness.
Duration: Duration of illness is dependent on health status of ind i-
viduals. Illness can present severe complications to pregnant women
and their fetuses (perinatal and neonatal infections), as well as
meningitis and septicemia in immune‐compromised persons and
the elderly. Fatalities occur from severe complications.
Inf. Dose: 102 to 103 CFU
Escherichia coli O157:H7
Source: Raw ground beef, raw seed sprouts, raw
milk, unpasteurized juice, raw vegetables,
contaminated water, food contaminated by
infected workers via fecal/oral route.
Growth: Grows with or without air; growth temp.
range: 44.6–114°F; 4.0–9.0 pH; 0.95 aw
Destruction
D [140°F] = 1.7 min.
Onset: 2 to 10 days (median 3 to 4 days)
Symptoms: Abdominal pain, diarrhea, stools are initially watery but
become grossly bloody. Vomiting occurs occasionally. Fever may
or may not be present.
Duration: The illness is usually self‐limiting and lasts for an average
of 8 days. Some individuals exhibit watery diarrhea only.
Complications: Hemolytic uremic syndrome, vascular and neuro-
logic complications in very young, elderly, and any immune‐
compromised individuals. Can be fatal.
Inf. Dose: Very low: 101 to 103 CFU
Vibrio parahaemolyticus
Source: Seafood, shellfish (shrimp, oysters), water.
Growth: Grows with or without air; growth temp.
range: 41–109.4°F; 4.5–11.0 pH; 0.937 aw
Destruction
D [120°F] = 0.35–0.72 min.
Onset: Illness occurs within 4 to 30 hours after consumption of
raw, undercooked, or cooked recontaminated fish and shellfish
harvested along the coast of the United States in warmer months
of year. Use of antacids increases susceptibility.
Symptoms: Abdominal pain, nausea, vomiting, diarrhea, fever, chills,
and headache. Systemic infection and death rarely occur.
Duration: Duration of illness is about 1 to 7 days.
Inf. Dose: 105 or more CFU
Shigella spp. (Shigella sonnei)
Source: Raw vegetables and herbs, non‐potable
water, any food contaminated by infected
workers via fecal/oral route.
Growth: Grows with or without air; growth temp.
range: 42.8–116.6°F; 4.8–9.3 pH; 0.95 aw
Destruction
Rapidly inactivated above 149°F (65°C).
Onset: 12 to 96 hours (usually 1 to 3 days)
Symptoms: Abdominal pain; cramps; diarrhea; fever; vomiting;
stools may contain mucous, pus, and blood.
Duration: Symptoms may last for 4 to 7 days, or may be prolonged,
depending on host factors, ingested dose, and strain. Fatality may be
as high as 10–15% with some strains. Reiter’s disease, reactive arthritis,
and hemolytic uremic syndrome are possible illness complications.
Inf. Dose: 10 to 100 CFU
(continued)
c13.indd 248 1/14/2016 7:33:58 AM
CHAPTER 13 • Food Safety and Spoilage • 249
Microorganisms and Source
(Atmosphere, Temperature Range for Growth,
pH Range, and Minimal Water Activity [aw])
Illness
(Incubation Period, Symptoms, Duration)
Yersinia enterocolitica
Source: Pork, raw milk, contaminated water supply.
Growth: Grows with or without air; growth
temp. range: 29.3–111°F; 4.6–9.0 pH; 0.945 aw
Destruction
D [145°F] = 0.24–0.96 min.
Onset: Illness occurs within 3 to 7 days (usually under
10 days) after ingestion. Often associated with consumption
of inadequately cooked or raw pork.
Symptoms: Gastroenteritis with diarrhea and/or vomiting; fever and
abdominal pain are common symptoms. May mimic appendicitis
and lymphadenitis. May cause reactive arthritis.
Duration: As long as 2 to 3 weeks. May be treated with
antibiotics other than penicillin.
Inf. Dose: 107 to 109 CFU
Staphylococcus aureus
Source: Humans are the main source of
staphylococci. Contamination of food can occur
by direct contact, indirectly by skin fragments,
or through respiratory tract droplets. Animals
and poultry also carry S. aureus.
Growth: Grows with or without air; grows in 10%
salt and produces toxin; growth temp. range:
42.8–118°F; 4.2–9.3 pH; 0.83 aw; Toxin
production: 50–114.8°F
Destruction
Cells:
D [160°F] = 15 sec.
Toxin:
D [210°F] = >2 hours
Onset: 30 minutes to 8 hours, usually 2 to 4 hours
Symptoms: Nausea, vomiting, retching, abdominal pain, diarrhea,
prostration
Duration: 1 to 3 days
Illness producing amount: Illness occurs from the ingestion of
ready‐to‐eat, potentially hazardous foods contaminated with
S. aureus that is allowed to grow and produce toxin when the food
is time/temperature abused.
Amount of toxin necessary for illness is produced by the growth
of 105 to 106 /g S. aureus in food, or consumption of <1 μg
enterotoxin.
SPORE‐FORMING PATHOGENS
Bacillus cereus
Source: Meat, poultry, starchy foods (rice,
potatoes), puddings, soups, cooked vegetables.
B. cereus is widely distributed and can be
found in soil, dust, air, water, and decaying
matter. Humans are not a significant source
of food contamination by B. cereus.
Growth: Grows with or without air; growth temp.
range: 39.2–122°F; 4.35–9.0 pH; 0.912–0.95 aw
Destruction
Vegetative cells: D [140°F] = 1 min.
Spores: D [212°F] = 5 min.
Two (2) types of illness:
(1) Emetic illness due to preformed toxin in food
Onset: 1/2 to 6 hours
Symptoms: Nausea, vomiting, occasionally diarrhea. (May resemble
S. aureus intoxication.)
Duration: Generally less than 1 day
Illness producing amount: Toxin produced by the growth of 105 to
1011/g B. cereus in food.
(2) Diarrheal illness results from the ingestion of vegetative cells
and/or spores and their subsequent multiplication and toxin
production within the intestinal tract.
Onset: 6 to 15 hours
Symptoms: Abdominal pain, nausea, watery diarrhea.
(Simulates C. perfringens gastroenteritis.)
Duration: 24 hours
Inf. Dose: >5 x 105 /g B. cereus in food
Clostridium perfringens
Source: C. perfringens is widely distributed
in soil, dust, and vegetation. It is found in
a large variety of foods including raw,
dehydrated, and cooked foods.
Cooked meat and poultry, casseroles, and gravies
made from cooked meat and poultry are often
implicated in outbreaks.
Growth: Grows with or without air; growth temp.
range: 59–125°F; 5.5–9.0 pH; 0.93–0.97 aw
Destruction
Vegetative cells: D [138°F] = 7.2 min.
Spores: D [210°F] = 26–31 min.; D [250°F] = 0.15 min.
Onset: 6 to 24 hours (usually 10–12 hours)
Symptoms: Abdominal pain, watery diarrhea. Nausea is common,
vomiting and fever are absent.
Illness results from the ingestion of vegetative cells and/or spores
and their subsequent multiplication and toxin production within
the intestinal tract.
Duration: 24 hours (Less severe illness symptoms can persist in
some individuals for 1 or 2 weeks.)
Inf. Dose: >105 to 1010 CFU
FIGURE 13.3 (continued)
(continued)
c13.indd 249 1/14/2016 7:33:58 AM
250 • CULINOLOGY®
Microorganisms and Source
(Atmosphere, Temperature Range for Growth,
pH Range, and Minimal Water Activity [aw])
Illness
(Incubation Period, Symptoms, Duration)
Clostridium botulinum (Type A and
Proteolytic B strains)
Source: Types A and B (proteolytic C. botulinum)
are found in soil and sediment. Type E is a
normal inhabitant of the marine environment.
Vacuum‐packed foods, reduced-oxygen
packaged foods, under‐processed canned
foods, garlic‐in‐oil mixtures, time/temperature-
abused baked potatoes/sautéed onions have
been implicated in outbreaks of Types A and B
(proteolytic C. botulinum).
Growth: Grows without air; grows in up to 5% salt;
growth temp. range: 50–118°F; 4.6–9.0 pH; 0.94 aw
Destruction
Vegetative cells: Killed in a few min. at 140°F
Spores: D [250°F] = 0.2 min.; (212°F) = 50 min.
‐‐‐‐‐‐‐‐‐‐‐‐‐
Clostridium botulinum (Type E and other
non‐proteolytic strains)
Source: Vacuum‐packed salted fish products
Growth: Grows without air; grows in up to 5% salt;
growth temp. range: 38–113°F; 5.0–9.0 pH; 0.97 aw
Destruction
Spores: D [180°F] = 0.49–0.74 min.
Toxin destruction (any botulinal toxin):
D [185°F] = 5 min.
Onset: 12 to 36 hours (range: 4 hours to 8 days)
Symptoms: Early signs of intoxication include lassitude, weakness,
and vertigo, usually followed by double vision and progressive
difficulty in speaking and swallowing. Difficulty in breathing,
weakness of other muscles, abdominal distension, and
constipation may also be common symptoms.
Duration: Can be fatal. Botulinum toxin causes flaccid paralysis
by blocking motor nerve terminals at the myoneural junction.
Flaccid paralysis progresses symmetrically downward, starting
with the face and eyes, to the throat, chest and extremities. When
the diaphragm and chest muscles become involved, respiration
is stopped and death from asphyxia results. Treatment involves
administration of botulinal anti‐toxin and intensive supportive care
(including mechanical breathing assistance). Recovery depends on
early treatment and care.
Toxic Dose: Up to about 109 LD50 toxin in mice. (LD50 = Lethal Dose
for 50% of population.)
VIRUSES
Norovirus
Source: Food contaminated by infected worker
via fecal/oral route, person‐to‐person transfer,
contaminated surfaces. Other sources: bivalve
shellfish, salads, water, and ice.
Growth: Does not grow in food.
Destruction: Resistant to heat inactivation.
Pasteurization does not eliminate virus. Steaming
shellfish may not inactivate virus.
Onset: Illness occurs within 24 to 48 hours (range: 10 to 50 hours)
after ingestion of microorganism in food or drink.
Symptoms: Nausea, vomiting, abdominal pain, diarrhea,
low‐grade fever, chills, general feeling of ill health, loss of appetite,
headache.
Duration: 24 to 48 hours
Inf. Dose: Unknown, probably less than 10 virus particles
Hepatitis A and E
Source: Shellfish, any food or water contaminated
by infected worker via fecal/oral route.
Growth: Does not grow in food or water.
Destruction: Resistant to heat inactivation.
Pasteurization does not eliminate virus.
Steaming shellfish may not inactivate virus.
Onset: Illness occurs within 15 to 50 days after exposure
(median = 28 to 30 days).
Symptoms: Fever, general feeling of ill health, loss of appetite,
tiredness, nausea, abdominal pain, jaundice.
Duration: If disease is mild, recovery is complete in 1 to 2 weeks.
If symptoms are severe, recovery and convalescence can take
several months.
Inf. Dose: Unknown, probably less than 100 virus particles
PARASITIC PROTOZOA
Giardia lamblia
Source: Human and animal feces, soil, water.
Water may contaminate food products when
used for irrigation or washing of fresh produce.
May be present in food or water as a cyst.
Destruction: Cysts
D [100°F] = 3 min.
Onset: Illness occurs within 3 to 25 days (7 to 10 days median time).
Symptoms: Diarrhea, abdominal pain, steatorrhea, bloating,
frequent loose and pale greasy stools, fatigue, weight loss. Can be
fatal in those unable to fight disease.
Duration: Usually 1 to 2 weeks. In some individuals, it may remain for
months to years.
Inf. Dose: 1 to 10 cysts
FIGURE 13.3 (continued)
(continued)
c13.indd 250 1/14/2016 7:33:58 AM
CHAPTER 13 • Food Safety and Spoilage • 251
Microorganisms and Source
(Atmosphere, Temperature Range for Growth,
pH Range, and Minimal Water Activity [aw])
Illness
(Incubation Period, Symptoms, Duration)
Cryptsporidium spp.
Source: Human and animal feces. May be
present in food or water as oocysts.
Destruction: Oocysts are destroyed at
temperatures above 163°F.
Onset: Illness occurs within 1 to 12 days (7 days median time).
Symptoms: Severe watery diarrhea, but may also be asymptomatic.
Pulmonary and tracheal cryptosporidiosis in humans is associated
with coughing, possible low‐grade fever, and severe intestinal
distress.
Duration: Usually 2 to 4 days. In some individuals, it may last
1 to 4 weeks.
Inf. Dose: 1 or more cysts
Confirmation: Identification of cysts and/or trophozoites
in feces.
Trichinella spiralis and other Trichinae
Source: Swine (pigs), wild game (bears, seals, etc.)
Destruction:
Trichinae are destroyed by heating meat
above 140°F.
USDA: Kotola: Heat to 170°F (adds extra level
of safety).
Onset: Illness occurs within 8 to 15 days (range: 5 to 45 days).
Symptoms: Some gastrointestinal symptoms; other symptoms are
dependent on organs or tissues affected. For example, muscle
soreness and pain with edema of upper eyelids is an early
symptom. These symptoms are followed by retinal hemorrhages,
pain, and photophobia. There is thirst, profuse sweating, chills
(fever), weakness, prostration, and a rapidly increasing number of
white blood cells.
Duration: Can cause severe illness and may be fatal if not treated
with medication (Mebendazole) in early stages.
Toxoplasma gondii
Source: Cats, soil; Toxoplasma gondii is an
obligate intracellular protozoan parasite.
Infected cats are the only species to shed
oocysts that sporolate in the environment in their
feces. Can be found in the soil and on food that
is grown in soil containing this parasite. Adults
become infected by consuming this parasite
from various sources in the environment.
Destruction:
T. gondii is destroyed by heating
meat above 140°F.
Onset: Illness occurs within 5 to 23 days after ingestion of
undercooked meat or outbreak associated with cats.
Symptoms: In adults, the infection may be without
symptoms, or present as acute disease with lympha denopathy
only, or resemble mononucleosis with fever, lymphadenopathy,
and lymphocytosis persisting for days or weeks.
A primary infection during early pregnancy may lead to
fetal infection with death of the fetus or manifestations
such as chorioretinitis, brain damage with introcerebral
calcification, hydrocephaly, microcephaly, fever, jaundice,
rash, and convulsions at birth or shortly thereafter.
Duration: Disease can persist for years in adults with varying
degrees of symptoms or lack of symptoms. The greatest threat
is the effect of life‐long brain damage to fetuses.
Inf. Dose: Unknown. No direct person‐to‐person transfer
except in utero. Oocysts shed by cats sporolate and become
infective 1 to 5 days later and may remain infective in water
and soil for more than a year. Cysts in the flesh of infected
animals remain infective as long as the meat is edible and
uncooked.
PARASITIC WORMS
Anisakis simplex
Source: Various infected fish (cod, haddock, fluke,
pacific salmon, herring, flounder, monkfish) that
are eaten raw or partially cooked
Onset: Within a range of 15 minutes to 26 hours after ingestion, with
a mean of 5 hours after ingestion
Symptoms: After ingestion of larvae‐infected fish, gastric symptoms
(abdominal pain, nausea, and vomiting) may occur. Occasionally
the larvae are coughed up. If the larvae pass into the bowel, a
severe response may also occur 1 to 2 weeks following infection,
causing symptoms mimicking Crohn’s disease. Gastroallergic
anisakiasis may also produce allergic symptoms, usually several
hours after ingestion.
Duration: Symptoms remain as long as this infective parasite is
present. Larvae may have to be removed surgically.
Inf. Dose: 1 larva
FIGURE 13.3 (continued)
(continued)
c13.indd 251 1/14/2016 7:33:59 AM
252 • CULINOLOGY®
Hazard Analysis
Critical Control Points
(HACCP)
HACCP is a process control system designed to identify and
prevent microbial and other hazards in food production. It
includes seven steps for preventing problems and for correct-
ing deviations as soon as they are detected. HACCP is
endorsed by such scientific and food safety authorities as the
National Academy of Sciences and the National Advisory
Committee on Microbiological Criteria for Foods (NACMCF),
and by such international organizations as the Codex
Alimentarius Commission and the International Commission
on Microbiological Specifications for Foods as the most effec-
tive approach available for producing safe food.
Under the Pathogen Reduction and HACCP Systems regu-
lations, the USDA requires all meat and poultry plants to
implement HACCP systems to monitor and control produc-
tion operations.10
The HACCP plan includes the analysis and control of biologi-
cal, chemical, and physical hazards—from raw material growth
and harvest, through procurement and handling, to manufac-
turing, distribution, and consumption of the finished product.
For successful implementation of a HACCP plan, management
must be strongly committed to the concept. A firm commitment
to HACCP by top management gives company employees a
sense of the importance of producing safe food.
HACCP is designed for use in all segments of the
food industry, from growing, harvesting, processing, manu-
facturing, distributing, and merchandising to preparing
food for consumption. Prerequisite programs such as
Good Manufacturing Practices (GMPs) are an essential
foundation for the development and implementation of suc-
cessful HACCP plans. Food safety systems based on HACCP
principles have been successfully applied in food processing
plants, retail food stores, and foodservice operations.
The seven principles of HACCP are universally accepted
by government agencies, trade associations, and the food
industry around the world. They are: (1) hazard analysis,
(2) critical control point identification, (3) establishment of cri-
tical limits, (4) monitoring procedures, (5) corrective actions,
(6) record keeping, and (7) verification procedures.
The Seven HACCP Principles
Principle 1: Conduct a hazard analysis.
Processors determine potential food safety hazards and then
identify preventive measures that can be applied to control
these hazards. A food safety hazard is any biological, chemi-
cal, or physical property that may cause a food to be unsafe
for human consumption.
Principle 2: Identify critical control points.
A critical control point (CCP) is a point, step, or procedure in
a food process at which control can be applied and, as a
result, a food safety hazard prevented, eliminated, or reduced
to an acceptable level.
Principle 3: Establish critical limits for each
critical control point.
A critical limit is the maximum or minimum value to which a
physical, biological, or chemical hazard must be controlled at
a critical control point to prevent, eliminate, or reduce it to an
acceptable level.
Microorganisms and Source
(Atmosphere, Temperature Range for Growth,
pH Range, and Minimal Water Activity [aw])
Illness
(Incubation Period, Symptoms, Duration)
Taenia (tapeworms and larvae)
Source: Raw beef and pork products; people
can become infected with the beef tapeworm,
Taenia saginata, and/or the pork tapeworm,
Taenia solium, after eating raw or insufficiently
cooked meat products. T. saginata is about 25
feet long, whereas T. solium is about 8 feet long
with up to 2,000 proglottids (segments).
Symptoms: The main damage is obstruction in the intestine. The
life cycle involves the release of gravid proglottids to soil, from
where they are consumed by cattle or pigs. Embryos are released
from the eggs and these are passed to the muscle of animal where
they encyst. These may then be consumed in poorly cooked beef
or pork. Most people with tapeworms have no symptoms, but
some report abdominal discomfort, diarrhea, and loss of appetite.
With severe infection, cysts in the brain and the tissues covering
the brain (meninges) in people with cysticercosis can result in
headaches, seizures, confusion, or other neurologic symptoms.
Rarely, cysts develop in the eyes, sometimes causing blindness, or
in the spinal cord, sometimes causing muscle weakness or paralysis.
Confirmation: Infection is recognized when the infected person
passes segments of proglottids in the stool, especially if the
segment is moving.
Sources: Adapted from CDC (2008)5; FDA (2009a)6; Heymann (2004)7; Smith Daifas, El‐Khoury, and Austin (2003)8; Smith Daifas, El‐Khoury,
and Koukoutsis (2002)9.
FIGURE 13.3 (continued)
c13.indd 252 1/14/2016 7:33:59 AM
CHAPTER 13 • Food Safety and Spoilage • 253
Principle 4: Establish CCP requirements.
Monitoring activities are necessary to ensure the process is
under control at each critical control point. The Food Safety
and Inspection Service (FSIS), an agency of the USDA, is the
public health agency responsible for ensuring the nation’s
commercial supply of meat, poultry, and egg products is safe,
wholesome, and correctly labeled and packaged. FSIS re –
quires that each monitoring procedure and its frequency be
listed in the HACCP plan.
Principle 5: Establish corrective
actions.
Corrective actions are those taken when monitoring indi-
cates a deviation from an established critical limit. Thus, a
plant’s HACCP plan must identify the specific corrective
actions to be taken if a critical limit is not met. Corrective
actions are intended to ensure that no product injurious to
health or otherwise adulterated as a result of the deviation
enters commerce.
Principle 6: Establish record‐keeping
procedures.
The HACCP regulation requires that all plants maintain cer-
tain documents, including hazard analysis and written
HACCP plans, and records the monitoring of CCPs, critical
limits, verification activities, and the handling of processing
deviations.
Principle 7: Establish procedures for
verifying the HACCP system is working
as intended.
Validation ensures that the plans do what they were
designed to do—that is, that they are successful in ensur-
ing the production of safe product. Plants are required to
validate their own HACCP plans. FSIS does not approve
HACCP plans in advance but rather reviews them for con-
formance. Verification procedures may include review of
HACCP plans, CCP records, critical limits, and microbial
sampling and analysis. FSIS requires the HACCP plan to
include verification tasks to be performed by plant person-
nel. Verification tasks are also performed by FSIS inspec-
tors. Both FSIS and industry undertake microbial testing
as one of several verification activities.
The success of an HACCP system depends on educating
and training each manager and employee in the importance
of their role in producing safe foods. Management must pro-
vide adequate time for thorough education and training.
Personnel must be given the materials and equipment neces-
sary to perform these tasks. It is important to recognize that
employees must first understand what HACCP is and then
learn the skills necessary to make it function properly.
Specific training activities include working instructions and
procedures that outline the tasks of employees monitoring
each CCP.
Hazards can enter the kitchen or processing facility from
personnel, the environment, the facility and equipment, and
from both food and non‐food supplies. Therefore, hazard
FOOD FACILITY
MANAGEMENT CONTINUOUS HACCP IMPROVEMENT
INPUT, H0
Prerequisite Processes
Personnel with disease
Environmental and facilities
hazards
Rats, mice, insects, birds
Air, dirt
Water contaminated with
microorganisms, and at a
high pH (>8.5)
Gas, solar, electric that can
be interrupted
Equipment cleaning and
maintenance
Supplies and material with
environmental and human
hazards, contamination,
various levels of nutrients
and spoilage
Receive, store
Food processes
Pre-preparation/wash
Preparation
Mise-en-place
Pasteurize
Fermented food:
cheese, buttermilk,
yogurt, wine, liquor,
sauerkraut, cider,
vinegar, etc.
Smoked:
meat, fish, poultry, etc.
Chemically preserved:
jams, dressing, nitrate,
acid, salt, sugar
Sterilized
Leftovers
Consumer
Serve—carry-out
Transport /package
Food with a good balance
among pleasure, safety,
nutrition, convenience
Consumer food abuse
Consumer sensitivities:
Allergies, intolerance,
etc.
Waste
Heat
Contaminated air
Sewage
Greywater
Glass, paper, cardboard
Metal, plastic
Food, grease
OUTPUT, FSO
Food with ALOP
FOOD HACCP PROCESS,
+ Σl – ΣR
Make the food safe to eat
FIGURE 13.4 HACCP‐based food system.
Used by permission of the Hospitality Institute of Technology and Management, St. Paul, MN.
c13.indd 253 1/14/2016 7:33:59 AM
254 • CULINOLOGY®
or reduced) to achieve a food safety objective (FSO) that
assures minimal risk (ALOP) while providing enjoyment,
nutrition, and convenience for the customer. Examples of
FSOs are “100 Bacillus cereus organisms per gram in cooked
rice” or “less than 100 mesophilic organisms per 50 square
centimeters on a clean cutting board.” These are deter-
mined by the company’s quality assurance department or
HACCP plan.
To develop foods that assure consumer safety, the devel-
oper must be able to (1) identify the hazards and specify
CCPs where hazards may increase, and then (2) specify con-
trols to reduce the chemical, physical, and biological hazards
on raw materials, personnel, and the kitchen/processing
environment so the product meets a hazard level considered
FIGURE 13.5 Potential chemical, physical, and biological hazards in food.
Chemical Hazards Physical Hazards Biological Hazards
Metabolic
Allergens
Food intolerance
Inborn errors of metabolism
Enzyme inhibitors
Protease inhibitors (e.g.,
haemagglutins, lectins)
Carcinogens
Naturally occurring in foods
Cyanogens
Result of cooking/processing
Acrylamide
Benzopyrene
Heterocyclic amines
Nitrosamines
Toxic compounds (toxins) in food
Fish/seafood toxins
Ciguatera
Scombroid poisoning
Shellfish toxins
Tetrodotoxin
Mushroom toxins
Other chemical hazards in food
Alcohol (Ethanol: added or formed
during fermentation)
Antibiotics
Artificial colors
Artificial sweeteners
Chemical contaminants from environment
Pesticide residues (pest control chemicals)
Heavy metals from food cooking and
storage equipment
Cleaning chemicals in the facility
Hormones
Excessive levels of preservatives
Excessive addition of nutrients (vitamins) and
other food chemicals such as MSG, sulfates
Lipid compounds
Olestra
Trans fat
Broken glass from bottles, jars,
utensils, uncovered light
fixtures, gauge covers
Wood from toothpicks,
wooden skewers, pallets,
boxes
Stones, metal fragments from
incoming supplies, utensils,
equipment, employees
Bone from improper processing,
supplies
Plastic from packaging
materials, pallets, equipment,
employees, surrounding areas
Personal effects from
employees
Pits and stems from fruit and
shells from nuts
Filth from birds, insects,
rodents, or any other
unwanted animal parts
Pieces of food that cannot be
swallowed (e.g., pieces of
meat, bone, hot dogs,
marshmallows, etc.)
Hot beverages and hot
solid foods that spill on skin
and burn tissue
Bacteria (vegetative cells and spores)
Vegetative, non‐exotoxin producer
Campylobacter jejuni
Escherichia coli O157:H7
Listeria monocytogenes
Salmonella spp.
Shigella spp.
Streptococcus
Vibrio spp.
Yersinia enterocolitica
Vegetative‐exotoxin producer
Staphylococcus aureus
Spore‐forming pathogens
Bacillus cereus (exotoxin)
Clostridium botulinum (exotoxin)
Clostridium perfringens
Viruses
Hepatitis A virus
Hepatitis E virus
Norovirus
Rotoviruses
Parasites
Giardia lamblia
Cryptosporidium parvum
Toxoplasma gondii
Trichinella spiralis
Cyclospora cayetanensis
Anisakis spp. and related worms
Others
Mold
Aspergillus flavus (aflatoxins)
Yeast
Candida albicans
Used by permission of the Hospitality Institute of Technology and Management, St. Paul, MN.
controls must be in place before HACCP‐based food produc-
tion begins to ensure hazards are reduced to an ALOP.
Food HACCP processes include:
1. mise‐en‐place (for example, gathering ingredients, opening
packages, washing food)
2. preparation (peeling, trimming, dicing)
3. use of food additives, where necessary for safety
4. cooking/pasteurizing to a safety critical limit
5. packaging
6. chilling or freezing as appropriate for later distribution
The hazards in food exiting the kitchen or the processing
facility must have been controlled (prevented, eliminated,
c13.indd 254 1/14/2016 7:33:59 AM
CHAPTER 13 • Food Safety and Spoilage • 255
an acceptable risk by the owner or quality assurance man-
ager of the business and that meets government health and
safety regulations.
The risk associated with each hazard is determined by its
frequency and its likelihood of causing severe illness or injury.
There is no zero risk. It is impossible to have a totally risk‐free
food production facility. However, if a new process or product
is developed without considering validated safe procedures,
the risk of causing consumer illness or injury is increased and
the manufacturer is subject to serious litigation.
Identification of
Hazards and Controls
To identify hazards and controls, research chefs and product
developers must know the sources of the hazards in the food
system as well as how to control them through correct meth-
ods of purchasing, storage, processing, and packaging. Food
manufacturers are responsible for buying from suppliers that
have HACCP programs or provide letters of guarantee for
food and ingredients. For fresh foods, this means the supplier
guarantees that foods have been grown, processed, pack-
aged, and labeled accurately and that the level of hazards in
the food meets a mutually agreed‐upon FSO. This is why
detailed ingredient purchase specifications are written.
Chemical Hazards
Chemical hazards may be present in food or ingredients as
purchased from suppliers, through cross‐contamination in
the production facility, or by mistakes in measurement or
identity. They can include allergens (for example, milk,
peanuts, seafood) and food intolerances (for example, gluten,
lactose, monosodium glutamate [MSG]); certain functional
ingredients (for example, aspartame, sulfites, nitrites) that
require warning labels on foods that contain them; and toxic
compounds found naturally in foods such as certain fish (for
example, tetrodotoxin, scombroid) and mushrooms (for
example, muscarine, psilocybin). Alcohol, antibiotics, and
some colors (for example, red dye #40, yellow dye #5) can also
be considered chemical hazards for consumers who have
dietary or religious restrictions or chemical sensitivities. Of
course, chemical contaminants such as pesticides, cleaning
compounds, heavy metals, and many other materials not
meant for human consumption are all considered chemical
hazards.
Preventing chemical hazards at unsafe levels from getting
into the food is the only control. Food processors cannot
remove hazardous chemical contaminants from food to
make the food safe. Product development and production
personnel must be trained to measure and use permitted
food chemicals (for example, MSG, phosphates, nitrates) cor-
rectly, and to store and use cleaning and sanitizing chemicals
and pesticides as directed by the manufacturer. They must
know the allergens or other ingredients (for example, gluten,
phenylalanine) that may cause metabolic disturbances in the
foods they produce.
Some chemical hazards are specific to fish. For example,
scombrotoxin (elevated histamine formation) in fatty fish
such as tuna, mackerel, and mahi mahi indicates spoilage due
to improper storage temperature control, and can cause a
classic allergenic response in sensitive individuals. Another
(continued)
FIGURE 13.6 Chemical hazards and control measures.
Hazard Control
Scombrotoxin from improperly cooled, stored cold
scombroid fish (tuna fish, mahi‐mahi, blue fish, anchovies,
bonito, mackerel)
• Check temperatures at time received. Scombrotoxin
is produced when fresh raw scombroid fish is
temperature abused.
Ciguatoxin from reef fin fish from southeast United States,
Hawaii, and other tropical areas (barracuda, jacks, king
mackerel, large groupers and snappers); due to an
accumulation of ciguatoxin in the muscle of fish when
they consume the dynoflagellate Gambierdiscus toxicus
• Purchase fish from suppliers that test for ciguatoxin.
Tetrodotoxin from pufferfish • Do not serve these fish.
Shellfish toxins
Paralytic shellfish poisoning (PSP) from molluscan shellfish from
northeast and northwest coastal regions (mackerel, viscera
of lobsters and Dungeness, tanner, and red rock crabs)
Diarrhetic shellfish poisoning (DSP) from molluscan shellfish in
Japan, western Europe, Chile, New Zealand, eastern Canada
Neurotoxin shellfish poisoning (NSP) from molluscan shellfish
Amnesic shellfish poisoning (ASP) from molluscan shellfish
from northeast and northwest coasts of North America;
viscera of Dungeness, tanner, red rock crabs, and anchovies
• Purchase molluscan shellfish from suppliers that have
a hazard control program.
• Purchase from suppliers who test their products and
certify the safety of the products.
c13.indd 255 1/14/2016 7:33:59 AM
256 • CULINOLOGY®
Hazard Control
Mycotoxins
Aflatoxin from mold growth (Aspergillus flavus) in corn and
corn products; peanuts and peanut products; cottonseed;
tree nuts such as Brazil nuts, pecans, pistachio nuts, and
walnuts; other grains and nuts also susceptible if conditions
allowing mold growth occur.
Patulin from the growth of Penicillium expansum in apple juice
• Purchase from suppliers who test their products and
certify the safety of the products.
• Check condition at time received. Do not use moldy
grains.
• Purchase from a supplier who will guarantee that
rotten apples were not used for juice production.
Poisonous mushroom species from numerous varieties of
wild mushrooms
• Buy mushrooms from regulated suppliers.
Pyrrolizidine alkaloids from plant foods containing these
alkaloids; most commonly found in members of the
Borginaceae, Compositae, and Leguminosae families
(herbal teas)
• Do not buy food or medicinals contaminated with
these alkaloids.
Phytohaemmagglutinin from raw red kidney beans;
undercooked beans may be more toxic than raw beans
• Soak in water for at least 5 hours. Pour away water.
Boil briskly above 200ºF in fresh water with
occasional stirring, for at least 10 minutes.
Environmental contaminants (e.g., pesticides, fungicides,
fertilizers, insecticides, antibiotics, growth hormones)
• Buy from suppliers who test, label and guarantee
safety of products.
• Store harmful chemicals in areas that are separate
from food supplies.
Chemicals used in retail establishments (e.g., lubricants,
cleaners, sanitizers, cleaning compounds, and paints)
• Store chemicals separate from food. Label and use
chemicals according to manufacturer directions.
Maintain Material Safety Data Sheets for all
chemicals. Use non‐toxic lubricants for equipment.
PCBs (Polychlorinated biphenyls) from packaging materials,
fish
• Buy from suppliers who test and will guarantee
safety of products.
Copper in copper containers, copper piping • Do not cook or store foods that are more acid than
pH 6.0 in copper containers/equipment.
• Use backflow prevention devices on beverage
vending machines.
Lead in galvanized containers that contain lead • Do not use cooking utensils or enamel ware
containing lead.
Preservatives and food additives
Sulfiting agents (sulfur dioxide, sodium and potassium
bisulfite, sodium and potassium metabisulfite) that are
used by processors for shrimp, lobster, wine, canned
lemon juice, dried fruits and vegetables
Nitrites/nitrates in cured meats, fish, any food exposed to
accidental contamination, spinach
• Buy food products from a supplier who can provide
product specifications on the levels of sulfiting
agents added to a product.
• Use and prepare products with no more than the
approved amount of (nitrites/nitrates) curing com-
pound.
Niacin in meat and other foods to which sodium nicotinate
has been added
• Sodium nicotinate (niacin is not currently approved
for use in meat or poultry with or without nitrites or
nitrates).
Monosodium glutamate MSG (flavor enhancer) has been
associated with Asian food but is found in many processed,
canned, and frozen foods
• Avoid using excessive amounts. (1 oz./100 lb. food
is safe.) Inform consumers of presence in food if
requested.
Allergens (milk, eggs, fish, crustacean shellfish, tree nuts,
wheat, peanuts, soybeans)
• Inform consumers of presence in food, when
requested.
Used by permission of the Hospitality Institute of Technology and Management, St. Paul, MN.
FIGURE 13.6 (continued)
c13.indd 256 1/14/2016 7:33:59 AM
CHAPTER 13 • Food Safety and Spoilage • 257
FIGURE 13.7 Physical hazards and control measures.
Hazard Control
Glass: Bottles, jars, glass light fixtures, utensils,
gauge covers, glass thermometer stems
May cause cuts, bleeding; may require surgery to find
or remove.
• Inspect and handle products in a manner to prevent
presence of hazards.
• Make certain that light fixtures are covered/screened.
• Do not use thermometers with glass stems.
• Account for broken glass objects/containers and discard
products if there is any possibility of broken glass.
Wood: Incoming raw foods, pallets, boxes, building
material, toothpicks, wooden spoons and paddles
used for mixing food
May cause cuts, infection, choking; may require surgery
to remove.
• Inspect incoming products for evidence of wood chips
or splinters and handle products in a manner to prevent
presence of hazard in prepared food.
• Use “toothpicks with pants” in food. Do not use wooden
utensils for food preparation and service. (Exception:
wooden stirrers can be used for candy making.)
• Remove objects or discard suspect food in order to
prevent injury.
Stones, rocks, dirt: Incoming foods such as uncooked
cereals and dried vegetable products (e.g., rice, navy
beans, fresh fruits and vegetables)
May cause choking, broken teeth; may require surgery
to remove.
• Designate supplier specifications for extraneous
contamination in rice and dried vegetable items.
• Inspect food products and remove stones as a part of the
preparation process. Wash fresh fruits and vegetables to
remove excess soil (dirt).
Pits and shell fragments: Fruit pits, shells from nuts
and shellfish
May cause choking, broken teeth; may require surgery
to remove.
• Inspect products for pits (e.g., cherries), shells from nuts
and shellfish (e.g., oysters). Remove pits/shells or discard
food in order to prevent injury.
Bone fragments: Meat, fish, and poultry products
May cause cuts, infection, choking; may require surgery
to remove.
• Inspect products for evidence of bones or bone splinters.
• Remove bone fragments or discard food in order to
prevent injury.
Metal fragments: Possible presence in food (e.g.,
buckshot, nails, bolts, pieces of equipment, wire
twist ties)
May cause cuts, infection, choking; may require
surgery to remove.
• Buy from suppliers who guarantee the safety of their
products.
• Inspect and handle products in a manner to prevent
presence of metal fragments in prepared food.
Plastic fragments: Packaging materials, equipment,
containers
May cause cuts, infection, choking; may require surgery
to remove.
• Inspect and handle products in a manner to prevent pres-
ence of plastic fragments in prepared food.
• Account for broken brittle plastic objects/containers and
discard food if presence is suspected.
Personal effects: Earrings, artificial fingernails, buttons,
clips, pins, pens, pencils, etc.
• Educate employees to not wear artificial fingernails and
any jewelry or other items that might fall into food.
• Inspect products and remove objects when presence is
suspected or discard food in order to prevent injury.
Human hair from head and beard
Animal hair
• Inspect products for the presence of human and/or
animal hair. The presence of hair in food is a quality
consideration. Consumers find it objectionable.
• Employees preparing food should be required to wear
hair restraints or coverings.
• Remove hair and serve food if contamination is minimal
or discard product.
Filth and pathogens from birds, insects, rodents, and
any unwanted animal parts
• Buy from suppliers who provide letters of guarantee of
the quality and safety of their products. A certain amount
of insect contamination is allowed by terms as defined by
Food Defect Action Levels (FDA, 1998). If safety and quality
of the product are in doubt, it should be discarded.
(continued)
c13.indd 257 1/14/2016 7:33:59 AM
258 • CULINOLOGY®
Hazard Control
Packaging defects: Broken boxes, bags, and other
packaging material that allows the entrance of dirt,
insects, and other contaminants
• Inspect package integrity when receiving food. Reject
broken packages. Inspect packages of stored foods.
• Discard any food items if broken packaging has
compromised the safety of food.
Sabotage: Deliberate contamination and compromised
safety of food products by employee(s) such as adding
broken glass, etc.
• Hiring and monitoring of employee performance.
• Good supervision practices.
• Discard food items if there is any doubt about food
safety.
Food asphyxiation hazards: Pieces of food that cannot
be swallowed, such as large pieces (probably larger
than 1⁄4 inch) of meat, bone, hot dogs, marshmallows,
etc.
• Cut food into smaller pieces, especially for young
children.
• Train employees in the use of the Heimlich maneuver
and call 911 when necessary.
Thermal hazards: Hot beverages and solid foods that
burn tissue, such as hot coffee, tea, soup, or jelly‐filled
Bismarcks heated in a microwave oven.
• Warn customers that products are very hot if served at
temperatures of 170°F (76.7ºC) and above.
Used by permission of the Hospitality Institute of Technology and Management, St. Paul, MN.
FIGURE 13.7 (continued)
example, ciguatoxin, may be present in reef‐dwelling species
such as grouper and snapper that have eaten toxin‐ containing
plankton called dinoflagellates (causative agent of “red tide”).
These fish require supplier testing at certain times of the year.
Finally, tetrodotoxin is a lethal poison found naturally in puff-
erfish, which are illegal to use as food in the United States.
Toxins from shellfish can cause paralytic, diarrheic, neuro-
toxic, or amnesic poisoning. Shellfish should be purchased
only from suppliers that certify it has been harvested from
safe waters, and all shellfish must be tagged with date and
location of harvest as well as the harvester’s name, address,
and FDA identification number.
Mycotoxins due to mold growth in foods (for example, afla-
toxin, ergotism) and poisonous mushrooms are also chemical
hazards and must be controlled by supplier certification.
Environmental contaminants and chemicals used in the
food facility, polychlorinated biphenyls (PCBs), and heavy
metals such as copper and lead come from a variety of
sources, and items containing these chemical hazards must
be assessed and then controlled or eliminated. Preservatives
and food additives can be controlled by not exceeding gov-
ernment‐mandated use levels in foods as indicated by the
supplier or by following government regulations. Finally, the
presence of allergenic or other ingredients the government
identifies as potentially harmful to certain members of the
population must be identified on food packages in clearly
written ingredient labels.
Physical Hazards
Physical hazards in food are conditions that can cause imme-
diate bodily injury as the food is consumed, such as broken
glass or wood, bones, stones, or plastic. Also included are
sharp objects that puncture the throat, large or hard food
ingredients that cause choking, and high food temperatures
that burn the skin.
When food manufacturing operations fail to keep physical
hazards out of food, the result is adulteration of food, defined
under Title 21, section 342 of the Federal Food Drug and
Cosmetic Act. Physical hazards can be prevented or elimi-
nated by examining and sorting the food (for example, dried
peas, dried beans, rice) for foreign objects. They can also be
prevented by purchasing from a certified supplier that uses
equipment such as a metal detector to remove metal frag-
ments and by providing strict ingredient specifications to the
supplier. If hot beverages such as coffee are served at 170°F
(76.7°C) or above, appropriate measures to prevent burns
include a hot food warning label and a coffee cup insulator
sleeve.
Expensive litigation can occur when customers discover a
foreign object in food or break a tooth, or even claim emo-
tional distress. They often have the food and the physical
hazard in their possession, which makes it difficult to refute.
In 1994, an elderly woman was awarded $2.9 million in a law-
suit against McDonald’s after she spilled hot coffee on her lap.
At the time, McDonald’s policy was to serve coffee at 180 to
190°F (82.2 to 87.8°C). They have since reduced that to 150 to
160°F (65.6 to 71.1°C).
Not all physical contaminants are necessarily hazardous.
The FDA’s Food Defect Action Levels (most recently updated
in July 2014) lists tolerable, unavoidable levels of physical
contaminants (for example, insect parts, rodent hairs) that
are impossible to eliminate from the food supply because “it
is economically impractical to grow, harvest, or process raw
products that are totally free of non‐hazardous, naturally
occurring, unavoidable defects.”11
c13.indd 258 1/14/2016 7:33:59 AM
CHAPTER 13 • Food Safety and Spoilage • 259
Biological Hazards
Biological hazards include foodborne pathogens such as bac-
teria, viruses, parasites, and some molds and yeasts that can
lead to illness, disability, and death by infection or intoxica-
tion (poisoning). These hazards cannot be totally eliminated
from incoming food, but they can be adequately reduced
with cooking and other processing procedures, such as
retorting, acidifying, refrigeration, the addition of antimicro-
bial ingredients, drying, modified atmosphere packaging
(MAP), and curing with high concentrations of salt or sugar.
More recent techniques include irradiation, high‐pressure
processing (HPP), pulsed high‐intensity light, ohmic proc-
essing (electrical field), ozonation (exposure to ozone), and
microwave processing.
The infection hazards include viruses (hepatitis A, hepati-
tis E, norovirus, rotaviruses), parasites (Trichinella spiralis,
Toxoplasma gondii, Taenia solium, Anisakis simplex, Cryp-
tosporidium parvum, Cyclospora cayetanensis), and vegetative
infective bacteria (Campylobacter jejuni, Salmonella spp.,
FIGURE 13.8 Biological hazards and control measures.
Microorganisms (Hazard Identification) Control
Viruses from human fecal contamination (on fingers
after using the toilet), shellfish, sewage leakage
into water; viruses cannot be reliably controlled
by cooking; must be prevented from contaminating
food
Hepatitis A
Hepatitis E
Norovirus
Rotoviruses
Controls target: Hepatitis A
– Double washing of hands and fingertips with a nail brush after
using the toilet (6‐log reduction).
– Exclude ill food handlers (norovirus).
– Hepatitis A vaccination
– Wash and sanitize food contact surfaces before processing
ready‐to‐eat food.
– Purchase seafood from suppliers who certify that their
products were obtained from safe waters.
Parasites in meat, fish, and poultry served raw or
undercooked; no USDA inspection control
Trichinella spiralis
Toxoplasma gondi
Taenia
Anisakis simplex
Controls target: Trichinella spp., Anisakis
– Freeze raw pork and wild game, fish at –4ºF for 7 days.
– Pasteurize (heat to 145ºF, 15 seconds for 5‐log reduction in
food muscle).
Parasites in fruits and vegetables
Cryptosporidium parvum
Cyclospora cayetanensis
Controls for Cryptosporidium parvum, Cyclospora cayetanensis
– Use safe (potable) water for growing and washing fruits and
vegetables.
– Obtain fruits and vegetables from suppliers who certify that
products were grown, harvested, handled, and packaged
under controls that prevent contamination.
– Surface pasteurize fruits and vegetables (160ºF, 1 minute).
Vegetative bacteria in raw meat, fish, poultry,
eggs, dairy products, fruits, and vegetables
Campylobacter jejuni
Salmonella spp.
Escherichia coli O157:H7
Vibrio spp.
Shigella spp.
Yersinia enterocolitica
Listeria monocytogenes
Controls target: salmonellae for pasteurization and
Campylobacter for surface cleaning
– Wash and sanitize ready‐to‐eat food contact surfaces (5‐log
reduction) (Campylobacter).
– Prevent cross‐contamination from raw food to ready‐to‐eat food.
– Use pasteurized ingredients, milk, dairy (e.g., juices, etc.).
– Pasteurize (heat/cook):
– Meat, fish to 150ºF, 1 minute for a Salmonella 5‐log
reduction.
– Poultry to 165ºF, 10 seconds for a Salmonella 7‐log
reduction.
– Maintain cold ready‐to‐eat food at 41ºF for 7 days or less for a
<3‐log increase in Listeria monocytogenes.
Vegetative bacteria that produce toxins in ready‐
to‐eat food
Staphylococcus aureus
Toxin produced by S. aureus is heat stable. Reheating
food to 165ºF does not ensure destruction of this
toxin. Prevent production of toxin in ready‐to‐eat
food.
Controls target: Staphylococcus aureus
- Wash hands before handling food and often when preparing
food.
- Clean and cover infected cuts and sores. Wear a clean plastic
glove to cover any cut(s) on a hand.
- Mix cold ingredients for salads <50ºF to control toxin
production of S. aureus.
- Hold food ≤41ºF (5ºC) or ≥130ºF (54.4ºC).
(continued)
c13.indd 259 1/14/2016 7:33:59 AM
260 • CULINOLOGY®
Escherichia coli O157:H7, Vibrio spp., Shigella spp., Yersinia
enterocolitica, and Listeria monocytogenes). (Note: The term
vegetative refers to actively living bacterial cells as distinct
from hibernating spores.) Other bacterial pathogens do not
normally cause illness by infection; instead, they are capable
of producing toxins in food that can cause illness or death
when consumed. These include Staphylococcus aureus,
Bacillus cereus, Clostridium botulinum, and Clostridium per-
fringens. The last three organisms are spore‐formers.
Spore‐forming bacteria such as Bacillus cereus, Clostridium
perfringens, and Clostridium botulinum are found in soils
throughout the world. They produce structures known as
endospores that protect the bacterial cell’s genetic material
when conditions are not conducive to survival of the living
vegetative cells; this process is known as sporulation. These
highly resistant structures (commonly known as spores)
are produced within the vegetative cell before it dies.
When conditions are favorable again, the spores germi-
nate and produce fresh vegetative cells that can grow and
reproduce as well as cause spoilage or disease. All raw and
even pasteurized and processed food from suppliers prob-
ably contain low levels of spores, but they become a risk
only when cooked food is improperly processed and han-
dled. If spores are allowed to germinate in these foods,
they produce dangerous, even lethal toxins. Clostridium
botulinum produces botulinum toxin, one of the most
toxic substances known.
Spores are noted for their marked resistance to heat, ultra-
violet (UV) light, chemicals, and desiccation, thereby making
control of them a challenge.12 Spores survive normal cooking
temperatures and become hazardous in improperly handled
low‐acid (pH above 4.6) cooked meat, fish, poultry, and vege-
tables dishes.
Inactivation of C. botulinum spores and prevention of toxin
production in low‐acid packaged or canned foods requires
sterilization by heating to a minimum of 250°F (121.1°C)
center temperature for three minutes. This results in a 12‐log
reduction of C. botulinum spores. In order to achieve 250°F
(121.1°C) in an aqueous (water‐based) food, packaged or
canned foods must be processed with 15 psi (pounds per
square inch) of pressure using a retort, autoclave, or pressure
cooker. Without added pressure, water can only reach 212°F
(100°C). To avoid any possibility of botulism, never use home‐
canned food in retail operations. Canned foods that show
rust, swelling, or signs of leakage must be discarded. Cooked
vegetables, such as potatoes and onions, must always be
refrigerated at 41°F (5°C) or below to prevent potential
growth of C. botulinum, which can also multiply in fresh
mushrooms at room temperature if packaged in an anaero-
bic (airtight) package. There must be air holes in the plastic
packages, and mushrooms should be stored refrigerated
below 41°F (5°C).
The growth of C. botulinum can also be controlled by acidi-
fying the food below pH 4.6 or by drying it to a water activity
lower than 0.92. The C. botulinum associated with fish, type E,
has a lower growth temperature threshold of 38°F (3.3°C).
Therefore, perishable fish items such as cold‐smoked fish
must be stored at or below 38°F (3.3°C) to prevent growth.
Because C. botulinum toxin is heat labile—that is, it can be
inactivated by heating—government agencies recommend
that all home‐canned, low‐acid foods for home use should be
boiled for 10 minutes before serving to assure safety.
Microorganisms (Hazard Identification) Control
Spore‐forming bacteria that survive cooking and
become potential hazards in cooked meat, fish,
poultry, and vegetables, prepared combination
items
Bacillus cereus
Clostridium perfringens
Clostridium botulinum
Toxin produced by any type of C. botulinum is
destroyed in 5 minutes at 185ºF. Boiling home‐canned
food assures safety.
Controls cooling, hot holding target: Clostridium
perfringens
- Hot hold >130ºF for <1 log increase of C. perfringens.
- Cool from 130ºF to 40ºF in less than 6 hours for <3‐log
increase of C. perfringens.
- Prevent contamination by Listeria monocytogenes or store
≤40°F or below, and consume within 7 days.
Controls target: Clostridium botulinum
- Do not use home‐canned food in retail operations.
- Reject/do not use canned foods that show rust, swelling,
and/or signs of leakage.
- Fresh vegetables (e.g., mushrooms) are packaged in bags
or containers that allow the entrance of air if they are stored
>50ºF (10ºC), at which point C. botulinum can make a toxin.
– Store prepared food products that have received a light heat
treatment and are labeled “keep refrigerated” at ≤41ºF (5ºC).
– Acidify food 130°F for enough time,
the pathogens that multiply are reduced to
undetectable levels.
At ≥130°F for adequate time, there is a 5‐log reduction of vegetative
pathogens (Salmonella) (not viruses, which take a higher temperature).
(FDA, 2005)
130°F 112 minutes
140°F 11 minutes
145°F 3 minutes
155°F 15 seconds
165°F 15 seconds (poultry, 7‐log Salmonella reduction)
Hot holding: The spores Clostridium
botulinum, Bacillus cereus, and Clostridium
perfringens survive pasteurization.
Holding food >130°F prevents outgrowth of spores. Adding acid to
reduce food to pH <4.6 or aw <0.92 controls Clostridium botulinum
and makes food shelf stable.
Cooling: Outgrowth of the spores must be
prevented.
USDA allows safe cooling from 120°F to 55°F in 6 hours with continued
cooling in the refrigerator (no time limit) to get to 40°F.
Cold display 41°F, 7 days for ready‐to‐eat food prepared in the retail food
establishment
Key: ºF (ºC):
30ºF (–1.1ºC); 41ºF (5ºC); 60ºF (15.6ºC); 130ºF (54.4ºC); 140ºF (60ºC); 145ºF (62.8ºC); 155ºF (68.3ºC); 165ºF (73.9ºC) 120 to 55ºF
(48.9 to 12.8ºC); 40ºF (4.4ºC); 41ºF (5ºC)
Used by permission of the Hospitality Institute of Technology and Management, St. Paul, MN.
c13.indd 262 1/14/2016 7:34:00 AM
CHAPTER 13 • Food Safety and Spoilage • 263
Receiving Temperatures
When food that requires Time and Temperature Control for
Safety (TTCS) is received, it should be stored at or below 40°F
(5°C), which is normal refrigerator temperature. However,
pathogens such as Listeria monocytogenes grow as low as 30°F
(–1.1°C), so if absolute safety is required (as when developing
foods for immunocompromised individuals, hospital feeding,
baby food, or extended space flight), unless the food will be
processed by other contamination elimination methods
shortly after receipt, food must be stored frozen at 30°F (–1.1°C)
or less. At 40°F (5°C), the spoilage microorganisms multiply
faster than the pathogens, and the food “spoils safe” (spoils and
is discarded before it becomes hazardous). If raw food is sold as
purchased from the supplier, the supplier must guarantee a
safe pathogen level. If pasteurized products (for example,
cooked meat, fish, poultry, and vegetables) are obtained from
suppliers, the suppliers must be ready to show HACCP plans
indicating that vegetative pathogens were reduced to a safe
level. Food temperature must be maintained continuously at
40°F (4.4°C) or below. Refrigerated shelf life depends on the rate
of germination and outgrowth of psychrotrophic spoilage
organisms—that is, bacteria capable of surviving or even thriv-
ing in a refrigerated environment.
Pre‐Preparation Temperatures
Pre‐preparation of food for a production run should normally
be completed in less than two hours, and the food tempera-
ture should not get above 60°F (15.6°C). This time is too short
at that temperature for more than one multiplication of a
vegetative pathogen and is not considered a risk factor. The
key to safety during pre‐preparation is the prevention of
cross‐contamination by the vegetative pathogens on raw
foods, on hands, and in the environment from contacting
ready‐to‐eat food.
Cooking/Pasteurization
Temperatures
Government regulations specify that cooking must provide a
5‐log18 to 6.5–7‐log19 Salmonella spp. reduction to assure a
safe level of vegetative pathogens in the food when con-
sumed. There can be some multiplication of pathogens, par-
ticularly C. perfringens, when cooking takes more than four
hours to get above 130°F (54.4°C)—for example, a luau roast
pig cooking in the ground overnight. However, once the tem-
perature is at or above 130°F (54.4°C), the pathogens that
multiplied are reduced to undetectable levels, given proper
time at that temperature, such as 150°F (65.6°C) for one min-
ute for a 5‐log salmonella reduction.
Hot Holding Temperatures
Hot‐holding food at or above 140°F (60°C) prevents spore ger-
mination and outgrowth and assures safety regardless of how
long the food is held. Food is also not hazardous and does not
need control if cooking has reduced the water activity to
lower than 0.92 (for example, bread and pastry), or if acid (for
example, vinegar, lemon juice) is added to reduce the pH to
4.6 or below (for example, béarnaise and barbecue sauces).
These foods can still undergo spoilage, however.
Cooling Temperatures
Cooking and hot holding do not damage bacterial spores;
rather, these processes activate them. When the temperature
of hot food drops below 130°F (54.4°C), spores begin to germi-
nate. In about two hours, vegetative pathogens begin to mul-
tiply at a rapid rate. The critical control is to cool rapidly
enough to allow no more than a 1‐log increase of C. perfrin-
gens. The FDA recommends 135 to 70°F (57.2 to 21.1°C) in
two hours, and 70 to 41°F (21.1 to 5°C) in no more than four
more hours for all non‐meat‐containing foods.20 The USDA
has two guidelines for cooling meats and meat‐containing
foods for food processors:21
1. “During cooling, the product’s maximum internal temper-
ature should not remain between 130°F (54.4°C) and 80°F
(26.7°C) for more than 1.5 hours nor between 80°F (26.7°C)
and 40°F (4.4°C) for more than 5 hours. This cooling rate
can be applied universally to cooked products.”
2. “All product should be chilled from 120°F (48°C) to 55°F
(12.7°C) in no more than 6 hours. Chilling should then con-
tinue until the product reaches 40°F (4.4°C); the product
should not be shipped until it reaches 40°F (4.4°C).” This can
be extrapolated to about 14.2 hours from 120 to 40°F (48.9 to
4.4°C) and is used for cooling large pieces of cooked meat.22
These steps are shown to be safe and to control C. perfrin-
gens to less than a 1‐log increase.
Cold Display Temperatures
Because there is no specified control for Listeria monocy-
togenes in a retail display case, it is presumed that, during
cooling, in‐store prepared or vendor‐supplied ready‐to‐eat
foods are potentially contaminated from environmental
sources such as countertops, floors, refrigerators, and coil
drips with this vegetative pathogen.23 To assure safety, these
foods should be stored for seven days only. In seven days at
41°F (5°C), there is not enough multiplication of L. monocy-
togenes in ready‐to‐eat food for it to reach hazardous levels of
more than 100 organisms per gram.
If a retail operator requires a longer food storage time, the
processing facility supplying that retail operation must be an
inspected processing plant and show control of L. monocy-
togenes. In a food processing plant, the food chilling and
packaging areas are regularly cleaned and sanitized accord-
ing to standard HACCP guidelines. Therefore, there should
be no detectable L. monocytogenes. The USDA and FDA pre-
sume that a packaged food is free of L. monocytogenes if none
is detectable in 25 grams of product. The manufacturer can
assign any reasonable shelf life appropriate to the food in
question, such as 30 days for potato salad at 41°F (5°C) or
40 days for chicken salad at 41°F (5°C), depending on the
formulation of the product. Usually, L. monocytogenes growth
inhibitors (for example, potassium lactate, acetic acid,
c13.indd 263 1/14/2016 7:34:00 AM
264 • CULINOLOGY®
calcium diacetate) are added to recipe formulations to pro-
vide extra safety.
Food Processes
Product developers should document safe procedures for the
preparation of food in formula recipes, flow charts, and
HACCP plans. The USDA, in 9 CFR 417, HAZARD ANALYSIS
AND CRITICAL CONTROL POINT (HACCP) SYSTEMS,
describes the five applicable food process groups, specified
controls, and shelf life characteristics for each one.
Group I Foods
This group includes non‐heat‐treated, non‐shelf‐stable foods
such as fresh mushrooms, leafy greens, melon, eggs from
chickens raised in a salmonella‐free environment, and sushi.
In recent years, many contaminated raw agricultural prod-
ucts have entered the food chain, making thousands of peo-
ple ill and causing several deaths. Therefore, some form of
intervention is strongly recommended. Vegetables can be
dipped in antimicrobial solutions (chlorine, paracetic acid,
and so on), eggs can be pasteurized, and raw fish can undergo
extended freezing to kill parasites.
Group II Foods
This group includes foods that are not fully cooked, with inhibi-
tors to make them shelf stable. This group includes foods with
low water activity (aw) and foods that contain enough acid to
lower the pH to a level that retards the growth of foodborne
pathogenic bacteria. Nuts, chocolate candy, spices and herbs,
oils, salted and dried fish, and dried pasta have a safe aw (an insuf-
ficient amount of water necessary for the growth of halophilic
[salt-loving] vegetative pathogenic bacteria such as S. aureus—
that is, an aw of less than 0.88). This group also includes low‐pH
foods (pH less than 4.6) that have undergone fermentation
through the growth of lactic acid bacteria and other alcohol‐ or
acid‐forming bacteria, including pepperoni, salami, olives, fer-
mented dairy products (cheese, yogurt, sour cream, buttermilk,
crème fraîche), sauerkraut, kimchi, beer, and wine. Other low‐pH
foods (salad dressings, coleslaw, salsa, condiments) contain vin-
egar (acetic acid), lemon juice (citric acid), or other acidifying
additives as allowed by government regulations.
FIGURE 13.10 Food groups for food safety process analysis.
HACCP Process Groups (USDA 9 CFR 417)
Prerequisite/GMPs Working Control Safety Shelf Life
I Not heat treated, not shelf stable (raw). Not
PHF (does not require TTCS) raw meat, fish;
sushi, sashimi; eggs, raw fruits and vegetables
Grown or supplier made safe,
with H
0
≤ FSO.
May require temperature control
for quality.
<14 days
(bacterial spoilage)
II Not fully cooked, with inhibitors to make shelf
stable.
Water activity control: nuts; chocolate candy; spices
and herbs; oil; salted, dried fish; fresh pasta
Fermentation control: Pepperoni, salami; olives;
dairy (cheese, yogurt, sour cream, milk/crème fraîche);
sauerkraut; kimchi; beer, wine
Acid control: Salad dressing; coleslaw; salsa; condiments
Grown safe, made safe by supplier,
with H0 that, with +ΣI − ΣR (5‐log
Salmonella reduction), meets FSO.
Does not require TTCS because of
product aw, pH, or additive
stabilizers.
<2 years, 70ºF
(21.1ºC)
(chemical spoilage)
III Fully cooked, not shelf stable. Hot or cooled,
refrigerated ready‐to‐eat food; meat, fish, poultry;
fruits, vegetables, dairy, pastry filling, pudding
Pasteurized (5‐log to 7‐log
Salmonella reduction) so that
H0+ΣI − ΣR meets or exceeds FSO.
Requires TTCS.
>130ºF (54.4ºC) safe;
<30ºF (1.1ºC) safe;
≤41ºF (5.0ºC), 7 days;
130 to 41ºF (54.4 to
5.0ºC), 4 hours toss
IV Fully cooked, with inhibitors to make shelf stable.
Marinara (tomato) sauce; fruit pie fillings; smoked
fish; pickled eggs; bread, muffins; pastry; packaged,
pickled, and low‐pH fruits and vegetables.
Pasteurized (5‐log to 7‐log
Salmonella reduction) so that
H0+ΣI − ΣR meets FSO.
Does not require TTCS because
of product aw, pH, or additive
stabilizers.
No shelf-life limit
V Commercially sterile, shelf stable. “Packaged”
meat, fish, poultry, fruits, vegetables, dairy/UHT milk
Sterilized, Clostridium botulinum
spores reduced 9 to 12 log.
Does not require TTCS.
No shelf-life limit
Key: PHF = Potentially Hazardous Food; UHT = Ultra High Temperature; H0 = Starting Hazard; FSO = Food Safety Objective; Σ = summary;
I = Increase; R = Reduction; TTCS = Time and Temperature Control for Safety; aw = water activity (available free water in product that is
able to evaporate from the food and affect the humidity around the product)
Used by permission of the Hospitality Institute of Technology and Management, St. Paul, MN.
c13.indd 264 1/14/2016 7:34:00 AM
CHAPTER 13 • Food Safety and Spoilage • 265
Note: Shelf life is based on loss of quality, not safety. Some
products are labeled “Refrigerate after opening.” This statement
indicates proper handling for the control of mold contamina-
tion and the minimizing of quality deterioration, not for safety.
Group III Foods
These are fully cooked, not‐shelf‐stable foods including
refrigerated ready‐to‐eat meat, fish, poultry, fruits and vege-
tables, dairy products, pastry fillings, and puddings. These
products are pasteurized at temperatures and times neces-
sary to provide a 5‐ to 7‐log salmonella reduction that meets
or exceeds the FSO (Food Safety Objective). Ready‐to‐eat
foods in this group require TTCS (Time and Temperature
Control for Safety). These foods must be kept above 140°F
(60°C) for safe handling during processing or below 40°F
(4.4°C) for safe storage. If ready‐to‐eat food is held at temper-
atures between 40 and 140°F (4.4 and 60°C), it must be dis-
carded after four hours. Ready‐to‐eat prepared foods can be
stored in a retail kitchen at or below 40°F (4.4 °C) after prepa-
ration or opening but must be used within seven days. If
longer storage time is desired, these foods must be frozen to
prevent the growth of L. monocytogenes.
Group IV Foods
This group includes fully cooked foods, with inhibitors to
make them shelf stable at room temperature. These products
include jarred marinara sauce, bread, muffins, pastry, fruit pies;
packaged smoked fish; and pickled low‐pH fruits and vegeta-
bles. These products are pasteurized at temperatures and
times necessary to provide a 5‐ to 7‐log salmonella reduction
that meets or exceeds the FSO. Foods in this group do not
require TTCS because of low product pH (less than 4.6), low
aw (less than 0.92), additive stabilizers, and protective pack-
aging. Deteriorative changes in the foods occur over time due
to oxidation (changes in moisture content and oxidative
changes decrease the shelf life of baked products to a few
days) or after the packaging is opened or removed. However,
these are spoilage issues; there is no shelf-life safety limit on
unopened acidified or low‐water‐activity products.
Group V Foods
These are commercially sterile, shelf‐stable foods that include
packaged or canned meat, fish, poultry; fruits and vegetables;
and UHT (ultra‐high‐temperature processed) milk. These
foods are sterilized to gain a 9‐ to 12‐log reduction of
C. botulinum spores. They do not require TTCS, and there is
no safety limit to the shelf life of these products if maintained
in intact packaging.
Ingredient HACCP
As research chefs or product developers gather ingredients for
the preparation of a recipe, they must consider the possibility of
each as a source of biological, chemical, or physical hazard that
must be made safe if the supplier has not already taken care of
it. Each item on the stock list must be evaluated to determine if
the supplier has pasteurized or sterilized the food or whether
the supplier has made the food shelf stable so it is non‐TTCS.
If the food must be made safe, the recipe must include a CCP
(Critical Control Point) step—the point at which the food pre-
parer prevents, eliminates, or reduces the hazard to an ALOP.
FIGURE 13.11 Menu item analysis.
Supplier Makes Safe Cook Makes Safe
Mozzarella cheese sticks
Potatoes: parfried, French fries, mashed
Onion rings, parfried
Corned beef hash
Sausage links, fully cooked
Ham, fully cooked
Veggie burger, fully cooked
Cheese pizza, fully cooked
Turkey breast, fully cooked
Smoked sausage
Roast beef, fully cooked
Eggs, liquid pasteurized
Macaroni and cheese,
fully cooked
French toast batter (pasteurized ingredients)
Pancakes (pasteurized ingredients)
Gravies (pasteurized ingredients)
Soups, fully cooked
Marinara sauce, fully
cooked, acid
Stuffing, fully cooked
Grits, oatmeal
Vegetables, frozen
Coleslaw mix
Canned fruits
Applesauce
Pico de gallo
Taco chips
Salsa
Cheeses
Cream cheese
Cottage cheese
Pickles
BBQ sauce
Butter, margarine
Spreads
Honey
Salad dressings
Condiments
Creamers
Coffees
Cocoa, coffee, tea
Fruit juices
Soft drinks (dispenser)
Milk
Assorted bread products
Apple crisp
Frozen pies (unbaked
and prepared)
Frozen cakes, brownies
Ice cream
Jams and jellies
Syrups (pancake)
Syrups (for malts,
sundaes, etc.)
Vinegar
Sugar (brown, powdered)
Crackers, croutons
Apple topping
Fresh fruits
Baked potato
Chicken parts and
strips
Chicken nuggets
Chicken breast
Chicken-fried steak
Cod, battered
Shrimp, breaded
Bacon
Hamburger
Eggs: in the shell, over‐
easy, up, hard‐boiled,
poached
Steak: T‐bone, sirloin,
country-fried steak
Fresh vegetables
Used by permission of the Hospitality Institute of Technology and Management, St. Paul, MN.
c13.indd 265 1/14/2016 7:34:00 AM
266 • CULINOLOGY®
A typical process for making food safe is thermal process-
ing, cooking, or pasteurization to reduce a biological hazard
to a safe level. Other products may receive an addition of acid
to lower the pH, undergo fermentation, or be dried to a water
activity of 0.92 or lower to control spore outgrowth.
The HACCP Recipe
or Formula
The HACCP recipe or formula is the document the research
chef or product developer writes to tell other food preparers
how to consistently make a menu item that meets a specific
sensory profile and attains an ALOP for the consumer.
The recipe begins with the assembly of the ingredients for
the menu item. Ingredients that cause allergic or intolerance
reactions are identified. Organic acids or other acidifiers can
be used to reduce pH to a desired level in the food. Salt and
sugar used to reduce water activity are verified. Foods that
are potential choking hazards (for example, bay leaves, pep-
percorns) are identified. Preservative levels (for example,
benzoate, sorbate) are checked to make sure they are suffi-
cient to do the job but do not exceed mandated limits.
FIGURE 13.12 Quality‐assured HACCP recipe procedure for beef stew.
Product: Beef Stew, Group III Portion size (vol./wt.): 1 cup (250 g) Preparation time: 3 to 4 hours
Written by: OPS Date: 9/09 Number of portions: 1000 Prepared by:
SA/QA by: SPO Date: 9/09 Final yield (AS): Supervisor:
Gp
#
Ing
# Ingredients and Specifications
Amount
EP Wt % VerifPounds Grams
I 1 Lean beef, cut in ¾‐inch cubes 224.00 101,606.4 40.34
2 Vegetable oil 6.80 3,084.5 1.22
II 3 Water (14 gallons) 118.00 53,524.8 21.25
4 Seasoned salt 2.00 907.2 0.36
5 Garlic powder 0.50 226.8 0.09
6 Bay leaf, crushed 0.50 226.8 0.09
7 Thyme 0.50 226.8 0.09
III 8 Carrots, sliced ¼ inch 60.00 27,216.0 10.80
9 Potatoes, cubed ½ inch 112.00 50,803.2 20.17
10 Onion, diced ¼ inch or small white onions 31.00 14,061.6 5.58
Total 555.30 251,884.1 100.00
Ingredients that could cause adverse allergic or intolerance reactions: None.
Management and Prerequisite Procedures (SSOP/GMP) are in place: Personal hygiene, environment/facility/
equipment cleaning, sanitation and maintenance, supplies.
Equipment: 200‐gallon kettle, pump fill station, dish‐package machine; sealing machine.
Pre‐preparation
1. Get pre‐cut ¾‐inch beef cubes (<41ºF) for stew. (20 minutes)
2. Get and measure vegetable oil and seasonings. (70ºF) (30 minutes)
3. Get and prepare (wash, peel, slice/dice, etc.) carrots, potatoes, and onions. Get and measure water. (≤70ºF)
(30 minutes)
Preparation
4. Brown stew beef in oil in steam jacketed kettle. (>200ºF surface, 20 minutes)
5. Add water and seasonings to kettle. (all ingredients >70°F, 15 minutes)
6. CCP Cover and simmer. (180–190ºF, 2 hours) (This reduces Salmonella >5 log.) Check the Beef Stew while it is
being cooked. If not at 180–190ºF, raise the heat. (The chef/manager should verify the correct cooking
temperature and/or corrective action on the recipe.)
7. Add carrots, potatoes, and onions and continue to simmer (180–190ºF) until vegetables are tender. (>30 minutes)
8. CCP Hold at >165ºF and pump/package into individual serving dishes. Seal. Rack and move to cooler. (30 minutes)
9. CCP Cool 120 to 55°F, 6 hours, and continue to cool until the stew temperature is 40°F.
10. Box. Store. 40°F.
11. Ship.
Verif
Key: ºF (ºC):
41ºF (5ºC); 70ºF (21.1ºC); 200ºF (93.3ºC); 180–190ºF (82.2–87.8ºC); 165ºF (73.9ºC); 120 to 55ºF (48.9 to 12.8ºC); 40ºF (4.4ºC)
Used by permission of the Hospitality Institute of Technology and Management, St. Paul, MN.
c13.indd 266 1/14/2016 7:34:00 AM
CHAPTER 13 • Food Safety and Spoilage • 267
Next, the product developer decides on a sequence of rec-
ipe steps. Vegetables are often peeled and washed the day
before use so they are ready to be added to the recipe. Sauces
are made the day they are to be used, before assembly of the
product begins. This way, all of the ingredients are ready
when the product is being built.
The recipe includes times and temperatures for each CCP
step. In addition, at each CCP, procedures for monitoring and
corrective actions are included. On the right margin of the
recipe, the manager of a food establishment can confirm by
recording his or her initials that he or she watched the pre-
parer perform each step and that all procedures were
followed.
This beef stew HACCP recipe example begins with cutting
up the beef and is followed by vegetable preparation.
Temperatures at these pre‐preparation steps are less than
70°F (21.1°C), and time is only 80 minutes, which is too short
for the hazard to be a significant risk. Next, the beef is
browned to above 200°F (93.3°C), which pasteurizes the sur-
face of the beef. However, the center of the beef cubes might
still be contaminated. Therefore, this is not a CCP because
we have not eliminated the possibility of pathogen survival
and the risk of causing disease. The meat is then simmered
for 2 hours to tenderize the beef. This simmering step pro-
vides a 6.5‐log reduction of Salmonella spp.; this is designated
a CCP because the step is essential to reducing the vegetative
pathogens to a safe level of less than 1 organism per 25 grams
of food. After the vegetables are added and the cooking is
complete, the stew is pumped into individual serving trays,
sealed, and cooled in a blast chiller. This cooling step follows
USDA guidelines: 120 to 55°F (48.9 to 12.8°C) in 6 hours, fol-
lowed by continued cooling to 40°F (4.4°C). This cooling pro-
cedure is another CCP because it prevents a more than 1‐log
increase of C. perfringens. When the stew reaches a tempera-
ture of 40°F (4.4°C), it is boxed and refrigerated in preparation
for shipping.24
The HACCP Flow Chart
The flow chart has the same steps and language as the recipe.
Isolating each step in a box clarifies the sequence of the steps
and the controls required at each. Ti is temperature into the
step; To is temperature out of the step; and t is the time it
takes to complete the step. The initial times and tempera-
tures are determined by the research chef or product devel-
oper when preparing the recipe in the test kitchen. However,
to confirm these data are correct for full production volumes,
full‐scale plant trials must be conducted to record any
changes due to scale‐up. Again, if a step prevents, eliminates,
or reduces a significant risk to an ALOP, the step is identified
as a CCP.
The HACCP Plan
The HACCP plan is the document the research chef com-
pletes after developing the recipe and flow chart. This docu-
ment is used by the FDA and USDA to evaluate the safety of
the process.
1. Get pre-cut 3/4-inch beef cubes (<41°F) for stew.
Ti <41°F To 50°F t 20 min.
Pre-preparation
Preparation
2. Get and measure vegetable oil and seasonings.
Ti 70°F To 70°F t 30 min.
3. Get and prepare (wash, peel, slice/dice, etc.) carrots,
potatoes, and onions. Get and measure water.
Ti ≤70°F To ≤70°F t 30 min.
4. Brown stew beef in oil in kettle.
Ti 50°F To >200°F surface t 20 min.
5. Add water and seasonings to kettle.
Ti >70°F To >70°F t 15 min.
9. CCP Cool 120 to 55°F, 6 hours, and continue to cool
until the stew temperature is 40°F.
Ti 120°F To 55°F t 6 hr. + 8 hr.
6. CCP Cover and simmer. (Reduce Salmonella >5 log.)
Ti >70°F To 180–190°F t 2 hours
7. Add carrots, potatoes, and onions. Continue to simmer
until vegetables are tender.
Ti 180°F To >180°F t >30 min.
8. CCP Hold at >165°F and pump/package into individual
serving dishes. Seal. Rack and move to cooler.
Ti 180°F To 165°F t 30 min.
10. Box. Store. 40°F.
Ti 40°F To 40°F t 1 day
11. Ship.
Ti 40°F To 40°F t —
CCP = Critical Control Point
Key: °F (°C):
41°F (5°C); 70°F (21.1°C); 200°F (93.3°C); 180–190°F
(82.2–87.8°C); 165°F (73.9°C); 120 to 55°F (48.9 to 12.8°C);
40°F (4.4°C)
FIGURE 13.13 Beef stew HACCP recipe flow.
Used by permission of the Hospitality Institute of Technology and
Management, St. Paul, MN.
In a processing plant, processes may be grouped into one
HACCP plan if all of the process controls are the same. For
example, beef stew, beef stew with vegetables, pork stew, and
lamb stew follow the same process and have the same CCPs.
Therefore, only one HACCP plan must be written for those
recipes.
The HACCP plan shows each step of the recipe process
(the sequence shown in the flow chart) in the first column,
and then includes the hazard and control details of each step
c13.indd 267 1/14/2016 7:34:00 AM
c13.indd 268 1/14/2016 7:34:00 AM
FIGURE 13.14 Beef stew HACCP plan.
Process Steps and Controls: GMPs
and prerequisites are in place
(Ti = temp. in; To = Temp. out;
t = Time to do the step)
B, C, P, Potential Hazards
and Risk Analysis
Control (Critical
Limit [CL] for each
Hazard Control)
Monitor & Record
(What, How,
Frequency, Who)
Corrective
Action & Record
Verification
& Record
(Procedures
and Frequency)
Pre‐preparation
1. Get pre‐cut 3/4‐inch beef cubes
(<41ºF) for stew
Ti <41°F To 50°F t 20 min.
B: Not significant. Vegetative
pathogens and spores are
controlled by low temperature
and short time.
C: None
P: None
2. Get and measure vegetable oil
and seasonings.
Ti 70°F To 70°F t 30 min.
B: Not significant. Vegetative
pathogens and spores are
controlled by low aw (water
activity).
C: None
P: None
3. Get and prepare (wash, peel,
slice/dice, etc.) carrots, potatoes,
and onions. Get and measure water.
Ti ≤70°F To ≤70°F t 30 min.
B: Not significant. Surface
contamination is removed
by washing and peeling
vegetables. Water is potable.
C: None
P: None
Preparation
4. Brown stew beef in oil in kettle.
Ti 50°F To >200°F surface t 20 min.
B: Not significant. Pathogens
on the surface of the meat are
destroyed by high browning
temperature.
C: None
P: None
5. Add water and seasonings to kettle.
Ti >70°F To >70°F t 15 min.
B: Vegetative pathogens and
spores. Time too short for
significant pathogenic growth.
C: None
P: None
6. CCP Cover and simmer. (Reduce
Salmonella >5 log.)
Ti >70°F To 180–190°F t 2 hr.
B: Vegetative pathogens
and spores
C: None
P: None
Cooking temperature
>165°F, >10 sec.
assures a >7‐log
salmonellae kill.
Assigned worker takes
lowest temperature
of center of food and
records on production
sheet for each recipe.
If temperature is
not >180°F, turn
up heat and/or
continue to cook.
Chef/manager
initials the
production
recipe record
each shift.
c13.indd 269 1/14/2016 7:34:00 AM
7. Add carrots, potatoes, and onions.
Continue to simmer until vegetables
are tender.
Ti 180°F To >180ºF t >30 min.
B: Not significant. Temperature
>130ºF controls spores and
kills vegetative cells.
C: None
P: None
8. CCP Hold at >165ºF and package
into individual serving dishes. Seal. Rack
and move to cooler.
Ti 180°F To 165°F t 30 min.
B: Clostridium perfringens.
Temperature >130ºF controls
spores and kills vegetative
cells.
C: None
P: None
Holding at >130ºF
prevents the
germination of the
Bacillus and Clostridia
spores.
Assigned worker verifies
the temperature of the
product in the cooking
device at the beginning
and end of pumping
and records it on the
log sheet.
If the temperature
drops below 165ºF,
the heat to the
cooking device is
increased, and a
note is written on the
production sheet.
The chef/
manager initials
the production
recipe record
each shift.
9. CCP Cool 120 to 55°F, 6 hours,
and continue to cool until the stew
temperature is 40°F.
Ti 120°F To 55°F t 6 hr. + 8 hr.
B: Pathogenic spores.
C: None
P: None
Cooling from 120
to 55°F in 6 hours
assures <1 log increase
of Clostridium.
perfringens.
The presence
of pathogenic
microorganisms from
cross‐contamination
is controlled by GMPs
and SSOPs.
Assigned worker
verifies that racks with
dishes are being
cooled correctly by
taking temperatures
hourly and recording
on log sheet.
If cooling goes off,
move racks to
freezer and continue
to cool until
refrigerator is fixed.
The cooling log
will be initialed
by the QC
supervisor at the
end of cooling.
10. Box. Store. 40°F.
Ti °F 40°F To 40°F t 1 day
B: None
C: None
P: None
11. Ship.
Ti 40°F To 40°F t —
B: None
C: None
P: None
Approved (QC) Date
Approved (Process Authority) Date
Key: ºF (ºC):
41ºF (5ºC); 70ºF (21.1ºC); 200ºF (93.3ºC); 180–190ºF (82.2–87.8ºC); 165ºF (73.9ºC); 180ºF (82.2ºC); 130ºF (54.4ºC); 120 to 55ºF (48.9 to 12.8ºC); 40ºF (4.4ºC)
Used by permission of the Hospitality Institute of Technology and Management, St. Paul, MN.
270 • CULINOLOGY®
in the columns beside the step. In the hazard column, the
biological, chemical, and physical (BCP) hazards for each
step are identified—or, if there is no hazard, this is identified
as None. This column also identifies whether or not the haz-
ard is a significant risk. If the risk is significant, the next col-
umn shows the control as a performance standard (for
example, 5‐log reduction of salmonella). The control valida-
tion reference is also included. In the following column, the
monitoring procedures are listed. Corrective action must be
shown in the next column if monitoring indicates the process
deviated beyond a critical limit. The final column indicates
how management verified the work—whether by direct
observation or by records review.
The beef stew HACCP plan shows steps #6, #8, and #9 as
the CCP steps. Step #6, Cover and simmer, is a CCP because
the stew’s cooking temperature, which reaches 180 to 190°F
(82.2 to 87.8°C), is above 165°F (73.9°C) for more than 10 sec-
onds, assuring a greater than 5‐log salmonella destruction.
Step #8, the pumping step, is a CCP to assure the food is hot
enough to prevent outgrowth of bacillus and clostridial spores.
Step #9 is the cooling step. For this product, as stated in the
HACCP plan, the standard of cooling from 120 to 55°F (48.9 to
12.8°C) in 6 hours with continued cooling to 40°F (4.4°C)
assures less than 1‐log increase of C. perfringens. The preven-
tion of pathogenic microorganisms from contaminating the
food is controlled by good manufacturing practices (GMPs)
and standard sanitation operating procedures (SSOPs).
Process Validation
The two stages for recipe development in a process plant are:
1. Initial product development in the test kitchen or food lab
using small‐scale equipment.
2. Scaling up production using large kettles, conveyor ovens,
cook tanks, and so on. It is important that scaled‐up prod-
uct have the same sensory profile as the development
kitchen’s product (the gold standard) and that product
safety, stability, and quality are achieved.
The first step is for the product developer to decide whether
to use government‐published microbiological destruction
information (for example, 5‐log reduction of salmonella) as
described in the FDA Food Code25 or the USDA’s Draft
Compliance Guidelines for Ready‐to‐Eat Meat and Poultry
Products26, or whether the process is sufficiently different that
he or she must develop a different validation and microbiologi-
cal kill study for the process. If government information is
used, the product developer must make a thermal time‐tem-
perature map of the production floor equipment and required
times and temperatures for pathogen destruction so as to
gather data showing the plant process meets regulatory
requirements.
Alternatively, the process can be tested by inoculating a batch
of food ingredients with a non‐pathogenic surrogate organism
such as E. coli ATCC 25922, which has similar death characteris-
tics as salmonella, and compare the number inoculated with the
number remaining in the finished product after the process is
complete to verify microbial destruction. The results of the
study are documented and become a part of the HACCP plan
for that particular product or group of products.
Food Spoilage
Food spoilage refers to food that is damaged or degraded to
the point where it is considered to be unfit for human con-
sumption. Food spoilage occurs in several ways, including
physical injuries to the food, insect and animal damage,
inclement weather effects, temperature storage abuse, the
natural result of over‐ripening or aging, and the normal meta-
bolic activities of microorganisms. Shelf life is defined as “the
time it takes for a product to decline to an unacceptable level
for consumption,” and the goal of the food industry is to
extend shelf life and create food that maintains quality and
edibility for a time that is adequate for distribution, stocking,
sales, and customer convenience.27
Biological food spoilage occurs when enzymatic changes
in foods occur or there is growth of yeast, mold, or acid‐
producing, non‐pathogenic bacteria (such as lactobacilli)
within the food, which can negatively impact its flavor, odor,
appearance, and texture. While ingesting spoiled food does
not typically lead to illness, the signs of spoilage are obvious
via smell and visual inspection, forcing food manufacturers
(and consumers) to throw away costly raw materials and
finished goods. It is interesting to note that some of the same
microorganisms that cause spoilage in some foods are
actually essential in others—for example, the organisms
responsible for providing fermentation and flavor develop-
ment in bread, wine, pickled vegetables, and dairy products.
Further, the growth of spoilage microorganisms can actually
inhibit the growth of some pathogenic bacteria, such as
Staphylococcus aureus in raw meat and poultry. In fact, fer-
mentation is one of the oldest methods used for preserving
foods, dating back thousands of years. (For a complete dis-
cussion of food fermentation, see Chapter 11.)
Signs of Food Spoilage and
Food Industry Consequences
We are all familiar with many signs of food spoilage: the fuzzy
growth in that forgotten jar of tomato sauce; the green shiny
sheen on deli meats; the brown discoloration on leafy pro-
duce; and the black mold on the edge of an unfinished yogurt
cup. These are household examples that are seen every day,
and while the household cost is usually minor, when similar
spoilage happens on a larger scale, the result is huge losses to
a manufacturing facility and potential damage to a com-
pany’s reputation. Food commodities that could have been
value‐added and sold at a profit are now destroyed.
Ubiquitous in Nature
The microorganisms capable of spoiling food are ubiquitous
in nature. They live in the soil, waterways, and air, and reside
on the skins of fruits, vegetables, animals, and humans. They
live inside the intestinal tracts of animals and can grow on
c13.indd 270 1/14/2016 7:34:00 AM
CHAPTER 13 • Food Safety and Spoilage • 271
improperly sanitized food processing equipment. They can
be transmitted directly to food via unwashed hands and dirty
utensils. Spoilage organisms seek opportunities to invade the
flesh of any plant or animal tissue that can provide the nutri-
ents and growth conditions they need to survive.
Types of Spoilage
Microorganisms
The microorganisms that cause spoilage include bacteria,
yeast, and mold. Bacteria are microscopic single‐celled
organisms with a relatively primitive cell structure. Bacteria
come in a variety of shapes and sizes and grow at a rapid rate
when conditions are optimal. Bacterial outgrowth cannot
normally be seen, but the resulting damage that occurs when
spoilage bacteria grow in food (bad odors and flavors, physi-
cal changes, and so on) is how we know spoilage has occurred.
Yeast and mold are both members of the fungi family and
share the advanced internal cell structure of plants and ani-
mals. Yeasts are single‐celled and reproduce by budding or
fission, while molds are multicellular and reproduce by
means of spores, which are produced in large numbers and
are small, light, and resistant to drying. They are thus easily
dispersed in the air; when they land on suitable nutrients,
they are able, under favorable conditions, to produce fresh
mold growth.28 Spoilage caused by mold is usually more visi-
ble than that caused by yeast and can be seen easily as it
grows and spreads on the surface of a food product.
Parameters of Food
That Lead to Spoilage
All of our food is of plant or animal origin. Therefore, to mini-
mize spoilage and maximize shelf life, it is important to
understand the intrinsic and extrinsic factors in foods that
affect the ability of microorganisms to grow. Intrinsic fac-
tors—those located within the plant or animal tissues—
include internal pH, moisture content, nutrient content, any
naturally occurring antimicrobial components, and overall
biological structure. Extrinsic factors include storage tem-
perature, relative humidity, and gas concentration in the
environment.29 These parameters may result in either multi-
plication or inhibition of microbial growth in foods.
Intrinsic Factors
pH
Increasing the acidity of foods, either through fermentation
or the addition of weak acids, has been used as a preservation
method since ancient times. In their natural state, most
foods, such as meat, fish, and vegetables, are slightly acidic,
while most fruits are moderately acidic. A few foods, such as
egg white, are alkaline. It is well known that groups of micro-
organisms have pH optimums, minimums, and maximums
for growth in foods. As with other factors, pH usually inter-
acts with other parameters in the food such as water activity
(aw), salt, temperature, redox potential (the likelihood for the
food or ingredient to become oxidized [lose electrons] or
reduced [gain electrons]), and preservatives to inhibit growth
of pathogens and other organisms. (Redox potential has an
impact on the types of bacteria that can grow on or in foods.
Not surprisingly, aerobes require an oxidized environment,
and anaerobes require a reduced environment.) The pH of
the food also significantly affects the lethality of the heat
treatment given to the food. Less heat is needed to inactivate
microbes as pH is reduced.
Another important characteristic of a food to consider
when using acidity as a control mechanism is its buffering
capacity. The buffering capacity of a food is its ability to resist
changes in pH. Foods with a low buffering capacity change
pH quickly in response to acidic or alkaline compounds pro-
duced by microorganisms as they grow. By virtue of their
amino acids, which have both acidic and basic areas on their
molecules, meats, in general, exhibit slightly better buffering
capacity than vegetables, and therefore require higher levels
of acidification to achieve a reduced pH than do vegetables.
Most microorganisms, particularly bacteria, grow well at a
pH of close to 7.0 (neutral). Yeast and mold can grow well in
more acidic environments (below 7.0). This explains why
most lower‐acid vegetables, meats, and dairy products are
spoiled by bacteria, while yeasts and molds cause spoilage in
the more acidic fruits and vegetables.
Moisture
Microorganisms need water in an available form to grow in
food products. The control of moisture content in foods is
one of the oldest preservation strategies. Food microbiolo-
gists generally describe the water requirements of microor-
ganisms in terms of the water activity (aw) of the food. The aw
of a food describes the degree to which water is bound in the
food, its availability to participate in chemical or biochemical
reactions, and its availability to facilitate growth of microor-
ganisms. The aw of pure water is equal to 1.0, and most fresh
foods have a water activity close to the optimum growth level
of most microorganisms (0.97 to 0.99).
The aw of a food product can be reduced via dehydration
or dry-heat cooking, or by the addition of solutes such as
sugar or salt, which molecularly bind to water and make it
unavailable for microbiological growth.
Most bacteria, yeast, and mold cannot grow below their
respective aw values of 0.90, 0.88, and 0.80, except for specific
extreme groups such as the halophilic/halotolerant (salt‐
loving or ‐tolerant) bacteria, which can grow at aw 0.75; the
xerotolerant (dry‐tolerant) molds, which can grow at aw 0.71;
and the xerophilic (dry‐loving) molds and the osmophilic (liv-
ing or thriving in a medium of high osmotic pressure) yeasts,
which can grow between aw 0.62 and 0.60.
31 Halophilic bacteria
(for example, Vibrio species) can spoil improperly salted meats,
and Staphylococcus aureus is a unique halotolerant pathogen
that can grow and produce toxin at an aw of 0.88. Xerophilic
molds target improperly dried meats. Both osmophilic yeasts
and molds can grow on damaged fruit surfaces, syrups, jellies,
and jams.
c13.indd 271 1/14/2016 7:34:01 AM
272 • CULINOLOGY®
2.5 3.5 4.5 5.5 6.5
Apples
Bananas
Limes
Melons
Oranges
Plums
Watermelon
Grapes
Butter
Milk
Cheese
Beef
Ham
Chicken
Fish and Shellfish
Oysters
Shrimp
Salmon
Asparagus
Beans
Beets
Cabbage
Celery
Olives
Red Onions
Parsnip
Potato
Pumpkin
Rhubarb
Spinach
Squash
Tomatoes
Turnips
FIGURE 13.15 Approximate pH values of some foods.
Source: Adapted from Jay, Loessner, and Golden, “Intrinsic and Extrinsic Parameters of Foods That Affect Microbial Growth” (2005).
FIGURE 13.16 Approximate pH growth range
of some spoilage microorganisms.
Organisms Minimum pH Maximum pH
Mold 0.00 11.00
Yeasts 1.50 8.50
Lactic acid bacteria 3.50 10.20
Acetobacter 4.00 9.00
Staphylococcus 4.00 9.50
Vibrio spp. 5.20 9.00
Note: Boundaries not precise; values dependent on other growth
parameters.
Source: Adapted from Jay, Loessner, and Golden, “Intrinsic and
Extrinsic Parameters of Foods That Affect Microbial Growth” (2005).
Nutrient Content
To grow and function normally, microorganisms require the
following: water, a source of energy (typically carbohydrates),
a source of nitrogen (proteins and amino acids), vitamins and
related growth factors, and minerals. Varying amounts of
these nutrients are present in foods. Meats have abundant
protein, lipids, minerals, and vitamins, but low levels of car-
bohydrates. Plant foods have high concentrations of different
types of carbohydrates and varying levels of proteins, miner-
als, and vitamins. Foods such as milk, milk products, and eggs
are rich in all nutrients.
Foodborne microorganisms can derive energy from carbo-
hydrates, alcohols, and amino acids. Most microorganisms
metabolize simple sugars, such as glucose. Others can metab-
olize more complex carbohydrates, such as starch or cellulose
(found in plant foods) or glycogen (found in muscle foods).
Some microorganisms can use fats as an energy source. In
general, only small amounts of minerals are required; thus,
many foods are good sources. The abundance of nutrients in
most foods is sufficient to support the growth of a wide range
of microorganisms. Those that predominate are organisms
that can most easily utilize the nutrients present.
Antimicrobial Constituents
A number of plant‐and animal‐based foods contain naturally
occurring antimicrobial constituents, but the usual concentra-
tion of these compounds in formulated foods is relatively low,
so the antimicrobial effect is slight. However, these compounds
may provide “an assist” when combined with other factors in
the formulation. Prime examples are the essential oils found in
oregano, thyme, cassia, lavender, and other herbs and spices.
The food industry can take advantage of these antimicrobial
properties by adding them to food to help retard spoilage. The
spices not only provide flavoring but also help to extend shelf
life. Another example is dried plums (prunes). The high levels
of organic acids and antioxidants in prunes are linked to the
suppression of microorganisms when they are mixed with
ground meat.34
Biological Structures
A food’s natural covering can protect it from damage, injury,
and microbiological spoilage. Eggshells, nutshells, banana and
apple peels, and animal hides are all examples of how the
natural structure, when intact, can prevent microbial attack
c13.indd 272 1/14/2016 7:34:01 AM
CHAPTER 13 • Food Safety and Spoilage • 273
thermophilic (heat‐loving) bacteria can cause spoilage in
retorted (pressure‐cooked) cans of food. It is incumbent
upon the producer to have specific knowledge of the food for-
mulation, processing parameters, expected storage condi-
tions, and the organisms most likely to be present when
formulating the best strategy for maximizing shelf life.
Relative Humidity
The humidity of the environment can affect the water activity
of the food being stored. The aw of a food can be raised in a
high‐humidity environment, allowing more spoilage organ-
isms to grow than in a dry, low‐humidity environment. This
can be counteracted with appropriate packaging.
Gases in the Atmosphere
Microorganisms have different sensitivities to oxygen. Those
that grow only in the presence of oxygen are referred to as
aerobic microorganisms; those that grow without oxygen are
called anaerobes; and those that grow with or without oxy-
gen are facultative anaerobes. A small group of microorgan-
isms, microaerophiles need a small percentage of oxygen to
grow well. Aerobic microorganisms require more oxidized
environments (more oxygen) than do anaerobic organisms,
FIGURE 13.17 Water activity of common foods.
Foods Water Activity (aw)
Fresh, raw fruits, vegetables, meat,
fish
>0.98
Cooked meat, bread 0.98 to 0.95
Cured meat products, cheeses 0.95 to 0.91
Sausages, syrups 0.91 to 0.87
Flours, rice, beans, peas 0.87 to 0.80
Jams, marmalades 0.80 to 0.75
Candies 0.75 to 0.65
Dried fruits 0.65 to 0.60
Dehydrated vermicelli, spices, milk
powder
0.60 to 0.20
Source: Farkas (2007)30.
FIGURE 13.18 Minimum water activity
for growth of foodborne microorganisms
at 77°F (25°C ).
Microorganism Group Minimum aw
Required
Many bacteria 0.91 to 0.88
Many yeasts 0.88
Many molds 0.80
Halophilic bacteria 0.75
Xerotolerant molds 0.71
Xerophilic molds, osmophilic yeasts 0.62 to 0.60
Adapted from Farkas (2007)32; Frazier and Westhoff (1988)33.
and spoilage. It is when these natural coverings are damaged
or removed that we see food spoilage. Factors that can reduce
effectiveness of protective coverings include deteriorative
effects of aging or ripening, insect or animal damage, bruising
from harvest or subsequent handling, temperature abuse, and
processing operations (peeling, slicing, dicing, and so on).
Extrinsic Factors
Temperature
Microorganisms are classified based on the temperature range
in which they grow best, with a minimum, maximum, and
optimum. Spoilage organisms are present at all ends of the
spectrum. An understanding of the interplay between time,
temperature, and other intrinsic and extrinsic factors is crucial
to selecting the proper storage conditions for a food product.
While most microorganisms are mesophiles, which grow
best at warmer temperatures (86° to 104°F [30° to 40°C]), psy-
chrophilic yeast and molds can grow well at refrigerated tem-
peratures as low as 25°F (–3.9°C), and some spore‐forming
FIGURE 13.19 Classification of bacteria based
on optimum growth temperatures.
Type of
Organism
Grows at These
Temperatures
Optimum Growth
Temperatures
Psychrotrophs Below 7°C
(45°F)
20 to 30°C
(68 to 86°F)
Mesophiles 20 to 45°C
(68 to 113°F)
30 to 40°C
(86 to 104°F)
Thermophiles Above 45°C
(113°F)
55 to 65°C
(131 to 149°F)
Source: Rachel Zemser.
FIGURE 13.20 Common examples of aerobic,
anaerobic, and facultative anaerobic spoilage
bacteria.
Food Type Aerobic Anaerobic Facultative
Meat Pseudomonas Clostridium Lactobacillus
Poultry Pseudomonas Shewanella
putrefaciens
Lactobacillus
Fish/
seafood
Pseudomonas Shewanella
putrefaciens
Lactobacillus
Vegetables Erwinia
carotovora
Bacillus
stearothermophilus
Lactobacillus
Fruit Yeast and
mold
Bacillus (canned) Lactobacillus
Source: Rachel Zemser.
c13.indd 273 1/14/2016 7:34:01 AM
CHAPTER 13 • Food Safety and Spoilage • 275
which require more reduced environments (lacking oxygen).
While most yeasts and molds are obligate or preferential
aerobes, spoilage bacteria have a wide range of oxygen needs.
Based on oxygen affinity, the growth of microorganisms
can be controlled by modifying the gaseous atmosphere
around the foods they inhabit. Gases inhibit microorganisms
by two mechanisms. First, they can have a direct toxic effect
that inhibits growth and proliferation. Carbon dioxide (CO2),
ozone (O3), and oxygen (O2) are gases that are directly toxic to
certain microorganisms. Oxidizing free radicals generated by
O3 and O2 are highly toxic to anaerobic bacteria and can have
an inhibitory effect on aerobes, depending on concentration.
Carbon dioxide is effective against obligate aerobes and at
high levels can deter other microorganisms.
Modifying the gas composition can also have an indirect
inhibitory effect by altering the competitive environment.
Atmospheres with a negative effect on the growth of one
particular microorganism may promote the growth of
another, which may provide a competitive inhibitory effect
on the spoilage organism or even against pathogens. This
effect may have positive or negative consequences depend-
ing on the native microflora and their substrate.
Controlled atmosphere (CA) and modified atmosphere
packaging (MAP) of certain foods can dramatically extend
their shelf life. Antimicrobial atmospheres have been applied
to fruits and vegetables, raw beef, chicken, and fish; dairy foods
including milk and cottage cheese; eggs; and a variety of pre-
pared, ready‐to‐eat foods. Controlling the gases in the sur-
rounding environment can be achieved in closed rooms during
bulk storage of raw produce or within the packaging of indi-
vidual food products; the procedure can both introduce an
assortment of gases or gas mixtures and remove gases entirely,
as in vacuum packaging.
Since 1928, fruits and vegetables have been stored in con-
trolled atmosphere warehouses containing increased levels
(up to 10 percent) of carbon dioxide as a way to slow, prevent,
and inhibit spoilage.35 Carbon dioxide retards the growth of
aerobic microorganisms. Ozone (O3) is used to prevent spoil-
age on beef surfaces. The choice of gases is determined by the
type of food, type of packaging, storage temperature and time,
and the spoilage organisms most likely to be present.
Any type of food processing has vulnerable points where
the process can fail, resulting in food spoilage. As a result,
preventive methods have been developed that are specifically
designed based on the food being processed and the growth
requirements of each particular microorganism. This is the
basis of HACCP plans.
Meat
Meat is a nutritious food that contains high levels of protein
and amino acids. In addition, the average pH of meat is 5.5 to
6.5, and the aw is approximately 0.99. Thus, meat is a perfect
growth environment for bacteria. The initial processing
steps, which include slaughter, hide removal, removal of
intestinal tract and viscera, washing the carcass, chilling, and
storage, must be done carefully to prevent contamination
with and outgrowth of both spoilage and pathogenic
microorganisms.
All animals have microflora residing inside the gastroin-
testinal tract and on the hide. The level of bacteria inside liv-
ing healthy muscle tissue is extremely low. However, during
slaughter, there are unlimited opportunities for cross‐
contamination to take place. When an animal is slaughtered,
it can fall on the floor and pick up bacteria from the floor and
walls of the processing environment. Cross‐contamination
can also occur during skinning, dehairing, and evisceration.
Other contamination can originate from the animal’s hide,
feces, dirty equipment, workers’ hands, and any contami-
nated contact surface. Lastly, when the carcass is stored and
transported, temperature control is critical in preventing the
outgrowth of microorganisms still present on the carcass.
Carcass Spoilage
The main cause of carcass spoilage is improper temperature
control. Under aerobic conditions, as storage temperatures
increase, so does the growth of psychrotrophic (cold-tolerant)
Food Type Bacteria Yeast Mold
Breads/bakery products Bacillus subtilis Rhizopus, Neurospora,
Penicillium, Aspergillus
Nuts, seeds, legumes NA/Rare NA/Rare Aspergillus, Penicillium,
Fusarium
Shell eggs Pseudomonas,
Acinetobacter, Proteus,
Aeromonas, Alcaligenes,
Flavobacterium, Escherichia,
Enterobacter, Staphylococcus,
Bacillus, Cytophaga
Torula Cladosporium,
Geotrichum, Mucor,
Rhizopus, Sporotrichum
Source: Rachel Zemser.
FIGURE 13.21 (continued)
c13.indd 275 1/14/2016 7:34:01 AM
276 • CULINOLOGY®
microorganisms such as Pseudomonas, Acinetobacter, and
Moraxella. If the surface of the meat is dry, the moisture‐loving
bacteria are inhibited, but yeasts and molds can still grow well.
If the carcass is stored under anaerobic conditions, such as
vacuum packaging, or with MAP, lactic acid bacterial growth is
favored, resulting in acid production that can alter flavor, tex-
ture, aroma, color, and water‐holding capacity of the meat.
Aerobic spoilage signs may include visible bacterial col-
ony formation (slime), shiny green surface, off odors, rancid
flavors, and visible surface mold growth. Anaerobic spoilage
indicators include sour, acidic, and cheesy odors and flavors.
Frozen Meat
If meat is properly frozen (at 14°F [–10C°] or lower), no micro-
bial spoilage occurs. However, mold can grow on meat held
as low as 23°F (–5°C) or higher, and lipid oxidation leading to
rancidity and freezer burn due to dehydration can still cause
spoilage during extended frozen storage.
Ground Meat
The aerobic surface of refrigerated ground meat can be
spoiled by psychrotrophs (bacteria that are capable of
growth in cold temperatures), and the internal anaerobic
center of the ground meat can be spoiled by lactic acid
bacteria. The FDA and USDA refrigeration requirement for
meat is 40°F (4.4°C) or below, and packaging is a CCP to
prevent cross‐contamination.
Cured Meats
Meat is cured by adding salt and nitrites (along with sodium
ascorbate to inhibit nitrosamine formation); it may or may
not be smoked. These procedures not only inhibit bacteria
but also create desirable flavors that redefine the meat into
specialized items such as ham, bacon, Genoa salami, and
corned beef. Curing prevents spoilage by increasing osmotic
pressure through the addition of salt (and consequent reduc-
tion of aw), and the nitrite has an antimicrobial effect, as does
smoking. In the manufacture of dry or semi‐dry sausages,
pure cultures of lactic acid bacteria are added to the ground
meat mixture (along with dextrose, a fermentable carbohy-
drate) to initiate fermentation, which has the additional anti-
microbial effect of reducing the pH.
During curing, the temperature must be kept cold; other-
wise spoilage can take place before the meat has reached an
aw or pH low enough to prevent outgrowth. Examples of
spoilage organisms include mold, Enterobacteriaceae, and
undesirable wild lactic acid bacteria—that is, not the ones
selected for the curing and fermentation process. Spoilage is
prevented by proper chilling, rapid curing, proper sanitation,
and, to prevent mold, storing the finished product in vacuum
or modified atmosphere packaging. Note that curing by itself
does not make the meat shelf‐stable. Additional hurdles,
such as drying, smoking, cooking, adding antimicrobial
agents, and refrigerated or frozen storage may be necessary
to provide the necessary protections against spoilage.
Dried Meats
Drying meats to an aw of 0.7 or below eliminates the possibility
of bacterial or mold growth. That said, certain types of Italian
and Hungarian dry sausages are inoculated with edible molds
(typically Penicillium spp.) to provide additional flavor devel-
opment and traditional appearance (white coating on the
surface).
Cooked Meats
Meats are cooked, packaged, distributed chilled or frozen,
and used by foodservice or sold by retail grocery establish-
ments. Again, temperature control is the key to spoilage
pre vention; if the product’s temperature is abused, psy-
chro trophs (aerobic) or lactobacilli (anaerobic) grow. If the
product is cooked in a vacuum bag (sous vide), spoilage is
typically via psychrotrophic spore‐forming anaerobes such
as Bacillus spp. or Clostridia. Post‐processing contamina-
tion of pre‐cooked products in conjunction with tempera-
ture abuse also contributes to microbial spoilage.
Retorted Meat Products
Low‐acid (above pH 4.6) meat products that have been
retorted—that is, have received a heat treatment sufficient
to kill the pathogen Clostridium botulinum—typically result
in a commercially sterile, room‐temperature‐stable prod-
uct. If there was spoilage outgrowth in the meat before the
retorting process, even though the processed product is
safe, remaining metabolites (undesirable heat‐stable com-
pounds created by the spoilage bacteria, such as acid and
gas formation) might make the product unpalatable. If the
heating process was not sufficient to kill the spores of
anaerobic spore‐forming spoilage bacteria (which are more
heat‐tolerant than C. botulinum), the canned product could
become spoiled if stored above 104°F (40°C), and germina-
tion of the spores took place. The usual offenders are two
members of the Bacillus group, known as flat sour organ-
isms because they cause sour taste and odor in the food
without any gas production, hence the ends of the can
remain flat.
Cooked and Cured
Perishable Meats
Processed meats such as sliced deli ham, hot dogs, bacon,
and canned hams are cooked and cured but still must be
refrigerated (or frozen) between 32 and 41°F (0 and 5°C) to
prevent spoilage. Spoilage may occur if the product is not
properly heat processed or is subsequently held above 41°F
(5°C). The amount of oxygen present determines which spoil-
age organisms predominate and what type of spoilage occurs.
Surface slime (from yeast and lactic acid bacteria), souring,
and greening are common. Greening occurs when lactic acid
bacteria produce hydrogen peroxide in the presence of oxy-
gen, which then goes on to oxidize the pink cured meat pig-
ment, turning it green.
c13.indd 276 1/14/2016 7:34:01 AM
CHAPTER 13 • Food Safety and Spoilage • 277
Cooked and Cured Shelf‐
Stable Meat Products
Spoilage occurs in these products (such as canned hams
and smoked sausages) if the canning process is insufficient
to kill all of the bacterial spores or if the sealed container
is compromised, leading to post‐processing contamination.
Bacillus spp. are usually the cause of spoilage in this type of
product.
Fish and Seafood
Fish and seafood includes marine and aquatic animals used
for human consumption: finfish, crustaceans, and mollusks.
Fish and seafood, like meat, are high in protein and moisture
and low in carbohydrate. In general, the internal pH of fish
falls between 5.8 and 6.7, providing ideal conditions for spoil-
age bacteria to survive and grow. All seafood tissues contain
compounds such as free non‐protein nitrogen (NPN), which
supports spoilage microorganism growth, and trimethyl-
amine oxide (TMO), which is reduced to trimethylamine (by
bacteria) and is responsible for the spoiled fish smell.
Crustaceans have slightly more carbohydrate and more
free amino acids than finfish. Mollusks have the most carbo-
hydrate of all—as much as 5.6 percent in oysters—which con-
tributes to their specific spoilage patterns as well.36
Fish Spoilage
The steps involved in fish processing include capture, cold
storage, evisceration, packaging, storage, and shipping. The
only way to inhibit fish spoilage is to maintain clean process-
ing conditions, tight temperature control, and good manu-
facturing practices. In general, internal fish muscle is sterile,
and spoilage bacteria reside on the skin, in the gills, and
inside the intestinal tract. Increased temperatures, combined
with unsanitary processing conditions, result in the micro-
flora interacting with the free amino acids in fish, leading to
rapid deterioration and spoilage.
Initial microflora counts depend on the origin of the fish
and the quality of the water in which they lived, including
temperature, salinity, and water pollution levels. The natural
microflora found on saltwater fish are typically halotolerant
psychrotrophs. Thus, these bacteria grow well in colder tem-
peratures over a salt range of 1 to 3 percent, which is the nor-
mal salinity of saltwater in various parts of the ocean and
estuaries. A wider variety of microbes can spoil freshwater
fish because there are no high salt levels to inhibit their
growth.
Spoilage Stages
Four stages are involved in fish spoilage when the product is
not handled properly (timing of each stage can escalate
quickly as the storage temperature increases):
Stage 1 (within 6 days after death) involves a population shift
from bacteria associated with live fish to those associated
with dead fish spoilage, but little odor.
Stage 2 (7 to 10 days after death) involves spoilage bacteria
growth and a noticeable fishy odor.
Stage 3 (10 to 14 days after death) involves rapid growth of
bacteria, strong fishy odors, and slime formation.
Stage 4 (more than 14 days after death) is when bacteria cease
to grow because the stationary phase is reached (the rate of
bacterial cell growth is equal to the rate of bacterial cell
death). The skin is very slimy, and the odor is putrid.37
Spoilage Microorganisms
In finfish, the main spoilage species are Shewanella and
Pseudomonas. In shellfish, spoilage is usually caused by
Vibrio species, as well as Pseudomonas and Acinetobacter‐
Moraxella. Mollusks are additionally spoiled by Flavobacterium
and Cytophaga. Yeast and mold are sometimes responsible
for fish spoilage, but this is uncommon.
Frozen raw seafood is whole, processed (broken down
into smaller cuts) or breaded raw seafood that is sold frozen.
Initial counts depend on the pre‐freezing processing condi-
tions and practices. Additional bacteria can be introduced
via unsanitary breading procedures. Bacterial counts increase
if the product is frozen slowly, thawed slowly, or held thawed
for too long. Yeast and mold growth can also occur if the fish
is held at improper frozen temperatures above 23°F (–5°).
Cooked frozen seafood is fish that is fully or partially
cooked (sometimes breaded) and then frozen. Cooked frozen
seafood undergoes a significant reduction in initial bacterial
counts, assuming that proper cooking methods are employed.
Post‐cooking, bacteria can be reintroduced during the
inspection and grading process. If the seafood is shipped
refrigerated instead of frozen, bacterial growth may occur.
Canned seafood, sterilized using the retort process, is free
of all spoilage bacteria, assuming process control is adequate.
However, much crabmeat is sold canned and pasteurized,
not sterilized, and must be refrigerated. It is not shelf‐stable
at room temperature, so one must pay close attention to the
label.
Cured and hot‐smoked seafood is processed by brining, fol-
lowed by smoke‐cooking at a minimum of 200°F (93°C) to an
internal temperature of at least 165°F (74°C). This process
coagulates proteins, kills vegetative cells, and reduces the fish’s
aw to less than 0.62, where microbial growth is inhibited. With
appropriate packaging, this type of seafood can be shelf‐stable
at room temperature. If it is cold‐smoked at temperatures in
the 80°F (27°C) range (commonly used for smoked salmon, or
lox), the seafood must be rapidly cooled and refrigerated to
prevent outgrowth of spoilage organisms because the low tem-
peratures of cold smoking permit the survival of a significant
portion of the natural microbiological flora on the fillets. This
is not necessarily a bad thing, as the spoilage organisms out-
compete potential pathogens such as Listeria and Clostridium,
causing the seafood to spoil (and be discarded) before it
becomes toxic. The opposite effect occurs if the cold‐smoked
fish is vacuum packed; the anaerobic environment reduces the
population of competing spoilage organisms enough to allow
c13.indd 277 1/14/2016 7:34:01 AM
278 • CULINOLOGY®
the Clostridium bacteria to proliferate, and toxin production
may occur before spoilage.38
Poultry
Chicken is a perfect growth medium for spoilage microorgan-
isms. It has an aw of 0.98 to 0.99 and a pH of approximately 5.7
to 6.7. Very high levels of spoilage microorganisms reside on
the living bird’s skin and feathers, although the internal meat
is typically clean until processing, at which point cross‐con-
tamination may occur.
Processing steps for chicken include slaughter, scalding,
defeathering, evisceration, spray washing, chilling, cutting into
parts, deboning, and packaging. Bacteria can be introduced at
each of these steps, especially if proper chilled temperatures
(<40°F [4.4°C]) are not maintained or if processing is done
in unsanitary environments. The primary spoilage organisms
isolated from chicken carcasses include Pseudomonas,
Acinetobacter, Flavobacterium, Corynebacterium, and low
levels of yeasts, molds, and Enterobacteriaceae. Yeasts and
mold typically grow only when antibiotics are used in the feed
to inhibit the growth of competing bacteria. In this situation,
one might encounter strains of Candida, Rhodotorula, and
Torula.
If properly refrigerated at <40°F (4.4°C), but without
atmosphere modification, raw chicken has a shelf life of 5 to
7 days. Vacuum‐packed or MAP chicken has an extended
shelf life of approximately 30 days. However, outgrowth of
anaerobic or facultative anaerobes such as Lactobacillus,
Shewanella, and Brochothrix may occur. Signs of spoilage
include sliminess (on the outer surface) and sour odor,
referred to as visceral taint. Usually, off odors are noticed
before the sliminess occurs. Whole birds and parts spoil at
about the same rate because they typically have the same
contamination level.
Frozen Chicken
When chicken is frozen properly and held at or near –0.4°F
(–18°C), there is very little spoilage. However, yeast and mold
can grow on frozen poultry stored at 19.4°F (–7°C), including
Cladosporum herbarum, which causes black spots, and
Thamnidium elegans, which causes whisker‐like growth.
Cooked Poultry Products
Cooked and ready‐to‐eat poultry products eventually spoil,
but the rate depends on how the product is processed and
stored. Cured and cooked chicken that contains sodium
nitrites spoils more slowly, as does cooked chicken packed
using vacuum or MAP packaging.
Dried Poultry Meat
Semi‐dried or intermediate moisture poultry products, such
as turkey jerky, do not support spoilage growth unless the
water activity increases above 0.60. At that point it is possible
for yeasts and molds to grow.
Vegetables
Vegetables include the edible parts of plants and the fruiting
body of mushrooms (fungi). All vegetables are naturally sus-
ceptible to spoilage, and it is estimated that 20 percent of all
fruits and vegetables harvested for human consumption are
lost to spoilage.39 Vegetables are very high in water and carbo-
hydrates and thus are excellent growth media for many kinds
of spoilage bacteria, yeasts, and molds. The amount of water
present inside a vegetable as well as the microbiological load
found in the soil, air, and surrounding water directly impacts
spoilage rates. Most bacterial spoilage is caused by Erwinia
carotovora, Pseudomonas, Corynebacteria, lactic acid–
producing bacteria, Clostridial spore formers, coliforms, and
micrococci. The dominating yeast spoilers include Rho-
dotorula, Candida, and Kloeckera apiculata, while molds
include Sclerotinia, Botrytis cinerea, and Aureobasidium pul-
lulans. Anaerobic vegetable spoilers are rare and usually
found in canned vegetables only.
Raw Vegetables
After harvest, vegetables may be shipped directly to market
raw and unwashed; cut, prepped, treated, and bagged; or
stored in temperature‐controlled and modified‐atmosphere
warehouses. Potential spoilage includes soft rot, spots, wilt,
and blight caused by Erwinia carotovora, pseudomonads,
xanthomonads, and Corynebacteria. Soft rot is easily identi-
fied by the soft, mushy appearance it gives the vegetables,
along with off odors and a water‐soaked appearance. It is
caused by the bacteria breaking down the pectins (structural
carbohydrates) inside the vegetables. Bacterial spoilage in
raw vegetables can be reduced during all stages of harvest
and storage by implementing proper sanitation and handling
methods, keeping the vegetables cold and dry, and washing
with chlorinated water.
Mushrooms are sold in many forms, including fresh, dry,
marinated, and canned. The main spoilage microorganisms
that can contaminate fresh mushrooms are Pseudomonas
fluorescens and yeasts and molds that come from the soil.
Fresh mushrooms should be stored dry and refrigerated until
ready for use. Storage in airtight plastic is not recommended;
instead, use cardboard boxes or paper bags with airflow
access (holes) to prevent the growth of potentially dangerous
anaerobes. Washing and blanching the mushrooms greatly
reduces the amount of contaminants, but they may be rein-
troduced during processing from dirty manufacturing facili-
ties. Mushrooms should not be washed too far in advance of
further processing because it increases the aw and hastens
the onset of spoilage.
Processed Vegetables
Raw vegetables for freezing are typically blanched first to fix
color, reduce the microbial load, inactivate enzymes, and
remove cellular gases, and then frozen for distribution and
sale. They are rarely spoiled by bacteria because the cold
temperature and low aw prevent spoilage. However, enzyme
c13.indd 278 1/14/2016 7:34:01 AM
CHAPTER 13 • Food Safety and Spoilage • 279
activity can continue at freezer temperatures if blanching is
insufficient, causing off flavor and off color development over
time. Inefficient, slow freezing and poor packaging can also
lead to surface dehydration (freezer burn) as well as dimin-
ished textural attributes.
Canned vegetables are thermally processed at sterilizing
temperatures (at or above 250°F [121°C]) to make them shelf‐
stable at room temperature. The exact temperatures and
times required for processing depend on the initial vegetable
pH and density (which affects the rate of heat transfer) as well
as the makeup of the brine or other packing liquid. Retorting
normally eliminates all spores. However, if processing is
insufficient, the most heat‐resistant thermophiles may come
out of their dormant spore state when the canned vegetables
are stored in hot environments (above 104°F [40°C]). This can
result in three types of spoilage:
1. Flat sour spoilage, which is acid production without gas,
caused by Bacillus stearothermophilus and Bacillus coagu-
lans. The ends of the cans do not swell; they remain flat.
2. Thermophilic anaerobic spoilage (TA), caused by the pro-
duction of hydrogen gas and carbon dioxide by Clostridium
thermosaccharolyticum. Cans become noticeably swollen
and spurt when opened.
3. Sulfide stinker spoilage, caused by hydrogen sulfide pro-
duced by Desulfotomaculum nigrificans. There is no gas
production, but there is a hydrogen sulfide odor (as of rot-
ten eggs) and possible black color if iron is present.
When commercial sterilization is done properly and cans
are stored under normal conditions (that is, not in tropical cli-
mates), unless the hermetic seal is breached or the can is punc-
tured, spoilage won’t occur until after the can is opened by the
consumer and subjected to post‐process contamination.
Dried Vegetables
Dried vegetables have their water activity reduced to less
than aw 0.85 to prevent the outgrowth of spoilage microor-
ganisms. Spoilage occurs only if the products are held at high
humidity, resulting in rehydration, which can result in yeast
and mold spoilage.
Fermented and Acidified
Vegetables
Vegetables have been preserved by salting, acidifying, or lac-
tic or acetic fermentation for thousands of years as a way to
preserve the harvest before refrigeration or other more
modern processing methods were known. Pickling reduces
the pH sufficiently to prevent the outgrowth of spoilage
organisms. Spoilage by yeast, lactic acid bacteria, coliforms,
and halophiles can occur in fermented, salted, and pickled
products if proper procedures are not followed. Spoilage can
be prevented by:40
1. Proper distribution of brine
2. Proper fermentation sequence
3. Maintaining appropriate temperature during fermentation
4. Destruction or inhibition of wild yeast activity
5. Using pure strain starter cultures
Fruits
Fruits are the seed‐bearing portions of plants. They are high
in water (85 percent) and sugars, but because of their low pH
(due to high levels of citric, malic, and tartaric acid), most do
not support the growth of spoilage bacteria.41 The few excep-
tions include olives, cucumbers, and tomatoes, which is why
these items are further acidified to lower the pH below 4.0, to
prevent spoilage.
Yeasts and molds, which grow over a wide pH range, are
the main spoilage organisms of concern in fruit and fruit
products. Yeasts can attack fruit sugars, causing fermenta-
tion and the production of alcohol and gas. Once the yeast
has partially broken down the fruit, molds are able to use the
alcohol by‐product metabolites as an energy source. Together,
they destroy the fruit.
Fruits typically have some initial microflora, especially on
the peel, which can be reduced during the primary process-
ing steps (including washing and peeling). General ways to
protect fresh fruit from microbial spoilage include using
approved fungicides prior to harvest, hot‐water surface
blanching, washing in chlorinated water post‐harvest, and
controlling the temperature, humidity, and gas atmosphere
of the fruit storage area. When all control measures are in
place, bacteria, yeast, and mold are greatly reduced.
Fresh Fruits
Citrus fruits are most commonly spoiled by Penicillium itali-
cum (blue rot) and Penicillium digitatum (green rot). Limes
and lemons are spoiled by Geotrichum candidum. Oranges
are spoiled by Alternaria citri, which causes the inside to turn
black. Post‐harvest spoilage control includes using deter-
gents, weak alkali, and fungicides. Keeping fruit surfaces dry
is the best strategy for preventing spoilage.
Apples and pears (pome fruits) are usually spoiled by
Penicillium expansum (blue rot), a cold‐tolerant organism.
Thus, cold storage only temporarily retards this type of spoil-
age. Botrytis cinerea causes gray rot in pears, displayed as ash‐
gray spore masses. Using a fungicide does not prevent all
pome fruit spoilage, but it does significantly inhibit the rot.
Stone fruits, which include peaches, plums, apricots, cher-
ries, and nectarines, are spoiled by brown rot caused by
Monilia fructicola, which causes water‐soaked spots on the
fruit that subsequently become brown. Transit rot occurs
when boxed fruit is transported in high‐humidity environ-
ments. Caused by Rhizopus stolonifer, it first produces soft rot
and then engulfs the fruit with a loose nest of mycelium. The
mold spreads rapidly and can quickly contaminate an entire
box of fruit. Cherries and plums are spoiled by Penicillium
expansum (blue mold). Spoilage can be controlled in stone
fruits by using a fungicide, maintaining the storage tempera-
ture below 40°F (4.4°C), and preventing bruising of the fruit.
c13.indd 279 1/14/2016 7:34:01 AM
280 • CULINOLOGY®
Berries, including blueberries, raspberries, blackberries,
and strawberries, are often spoiled by the molds Botrytis
cinerea and Rhizopus stolonifer. Spoilage in berry fruits can be
prevented with pre‐harvest antifungal sprays, careful han-
dling, and prompt refrigeration.
When certain wine grapes are infected with the fungus
Botrytis cinerea, they begin to rot and shrivel, concentrating
their sugars and creating a desirable flavor profile in wines
produced from them (French Sauternes, Hungarian Tokaji,
and some German dessert wines)—among the most sought‐
after and expensive wines in the world. This type of spoilage
is therefore known as noble rot. However, when Botrytis
cinerea infects regular table grapes, significant spoilage
occurs. In general, all kinds of grape spoilage can be pre-
vented by using pre‐ and post‐harvest antifungal sprays and
rapid transfer after picking into cold storage.
With a pH range of 4 to 4.5, tomatoes are typically spoiled
by mold. Examples of tomato spoilage include Alternaria rot
(dark brown and black lesions caused by Alternaria alter-
nata), chilling injury, which allows spoilage by the fungus
Cladosporium herbarum, and gray mold rot due to Botrytis
cinerea. Sour rot is caused by Geotrichum candidum and soil
rot by Rhizoctania solani. To prevent or delay spoilage, it is
important to practice good sanitation and use appropriate
fungicides and pre‐harvest sprays. Tomatoes for retail sale
are typically picked green, or at least under‐ripe, for transport
so as to reduce transport injury and subsequent colonization
by spoilage organisms. Not only are green tomatoes less easily
damaged but also acid levels are at their peak, inhibiting
microbial growth. That said, they are also lacking in flavor,
which never quite achieves the picked ripe taste of tomatoes
allowed to ripen on the vine.
Frozen Fruits
Any microflora left on fresh fruit after it is picked, harvested,
washed, and frozen remains during freezing. The cold temper-
atures prevent outgrowth of any yeasts and molds. However,
improper or prolonged thawing can lead to spoilage.
Canned Fruits
A combination of a naturally low pH (below 4.6) and a pas-
teurizing heat process usually prevents the outgrowth of
spoilage microorganisms in canned fruit. Hot‐fill‐and‐hold or
hot‐water bath/hot‐steam bath processing is normally all
that is required for most fruits, with temperatures of 175 to
210°F (79.4 to 98.9°C), depending on pH, being sufficient to
kill all microorganisms except bacterial spores. Because the
spores do not germinate due to the low pH, the food is con-
sidered commercially sterile.
High‐acid foods may be processed in a hot‐water or steam
bath at atmospheric pressures; no pressure‐cooking or retort-
ing is required. This heating requirement applies not only to
fruits but also to all acidified foods that are heat‐processed in
cans for long‐term shelf storage, such as pickled vegetables
(cucumbers, beets, cocktail onions, cherry peppers, olives)
and salsa. An acidified food is a low‐acid food (pH above 4.6)
to which acid(s) or acid food(s) are added to reduce its “equi-
librium pH” after mixing together to below 4.6, thus prevent-
ing the growth of harmful bacteria and extending shelf life.
Spoilage in canned fruits is rare, but when it does occur, it
is due to butyric acid–producing or thermophilic (heat‐
loving) anaerobic spore‐formers. Clostridium pasteurianum
can grow in a pH of 3.8, causing spoilage of pears by produc-
ing acid and gas. Under‐processing tomatoes can result in the
outgrowth of thermoduric (heat‐tolerant) facultative anaer-
obes such as Bacillus coagulans, which results in flat sour
spoilage. This spoilage is known to occur in tomato juice as
well; the acid‐producing bacteria reduce the pH while pro-
ducing off flavors described as being medicinal and oddly
fruity.
Dried Fruits
Dried fruits include sulfured, unsulfured, moist packs, and
glacéed fruits. Sulfured fruits have added sulfur dioxide,
which preserves the fruit’s color (bright orange dried apri-
cots are the best example) by preventing enzymatic brown-
ing and by eliminating all microflora. The government
requires that any foods containing sulfites at greater than
10 ppm must have a warning label for individuals with sulfite
sensitivity. Unsulfured fruits are dried naturally without sul-
fur dioxide (dried unsulfured apricots are dark brown).
Moist‐pack fruits are rehydrated in hot water to an aw of 0.85
to 0.90 before packing; and glacéed fruits are infused with
glucose syrup and sulfur dioxide. When sulfur dioxide is
used, spoilage is rare. However, in the absence of sulfur diox-
ide, dried fruits are susceptible to spoilage by xerophilic
fungi if they are not dried and stored properly. In glacéed
fruits, free sulfur dioxide is bound by glucose. Hence, some-
times the yeast Schizosaccharomyces pombe, which can grow
at low aw and is resistant to sulfur dioxide, can cause spoilage
during certain points in the glucose infusion process.
Fruit Juices, Concentrates,
and Preserves
Fruit juices, fruit concentrates, and preserves are products
that combine fruits with other ingredients such as sugar,
water, gums, acids, pectins, and corn syrup to create a final
processed product with varying amounts of actual fruit. The
surface of raw fruits used to make these products carry natu-
rally occurring yeast, mold, and bacteria; these can cause
product spoilage if not inhibited by acid, sugar, heat process-
ing, or preservatives. The two main factors that contribute to
a fruit product’s shelf stability are its water activity (aw) and
acidity (pH); depending on the process or additional ingredi-
ents used, these may require adjustment to prevent out-
growth. Water activity can be controlled by increasing the
product’s Brix (the total measured soluble solids in solution);
this is accomplished by adding sugar or corn syrup. As the
Brix increases, the water activity decreases, preventing bacte-
rial outgrowth and subsequent spoilage. Because both yeast
and mold can grow in acidic environments, fruit products
c13.indd 280 1/14/2016 7:34:01 AM
CHAPTER 13 • Food Safety and Spoilage • 281
must be acidified well below pH 4.0; this measure must be
combined with heat treatment and possibly preservatives
such as sorbic acid or benzoic acid to prevent post‐ processing
outgrowth.
Fermented and
Acidified Fruits
Certain fruits we think of as vegetables are actually fruits,
notably olives, cucumbers, and tomatoes. These fruits are not
very acidic (even some tomatoes) and must be treated differ-
ently than those that typically fall well below pH 4.6.
Olives are fermented by their own natural microflora (a
combination of yeast, bacteria, and mold), with Leuconostoc
being one of the important bacteria in the process. Before
fermentation begins, the raw olives are treated with lye (to
remove bitter flavors). If the lye is not leached properly from
the olive, it can inhibit Leuconostoc and allow spoilage organ-
isms to prevail, causing gas, bloating, and cheesy odors.
Cucumbers are preserved by salting, lactic fermentation,
direct acidification, and (in jarred pickles) pasteurization. If
improper brining, acidification, or pasteurization is used,
outgrowth of yeasts and halophilic spoilage bacteria can
occur, resulting in soft, bloated, gassy cucumbers.
Tomato products have their own unique set of potential
spoilage issues due to inconsistent pH levels that do not
always fall below 4.6. Typically, tomatoes are acidified with
citric acid if they are not below 4.6 and are going to be further
processed in cans, jars, bottles, and pouches. Spoilage occurs
if proper heat treatment is not applied and Clostridium or
Bacillus coagulans spores survive and cause flat sour spoil-
age. Machinery mold caused by Geotrichum candidum can
occur when manufacturing plants do not practice sound
sanitation and mold spores from the processing equipment
contaminate the tomatoes.
Spices and Herbs
Spices and herbs are aromatic plant materials used to sea-
son or flavor food. They are available in whole or ground
form, fresh, frozen, or dry, and as extracted essential oils and
oleoresins.
The initial microbial load of spices is typically very high
because they are agricultural products. A wide variety of
microorganisms can be found on spices, including aerobic
and anaerobic spore‐formers, psychrotrophs, thermophiles,
yeast, and molds. These organisms do not generally cause
spoilage of the herbs and spices after drying because they are
inactivated by the drying process. Of greater concern is what
happens when contaminated dried herbs or spices are used
to season other foods, where they are exposed to moisture,
nutrients, and, in some cases, heat. Given the right environ-
ment, with sufficient moisture and a plentiful food source,
bacterial and fungal spores can germinate and spoilage may
occur. Dry spices and herbs are normally treated to reduce or
eliminate spoilage organisms using chemical fumigants,
steaming, or irradiation.
Cereal Products
Cereal grains include wheat, corn, oats, rye, rice, barley, and
millet. These are manufactured into cook‐up or ready‐to‐eat
breakfast cereals, flour, bread and other baked goods, pasta
and noodles, tortillas, beer, sake, and vinegar.
Cereal products contain carbohydrates, proteins, fats,
minerals, and vitamins. They have a neutral pH, are an excel-
lent energy source, and provide an ideal growing environ-
ment for spoilage microorganisms—except that in their
normal dried state, they lack sufficient water. Typically, cereal
products are dried to a water activity level below 0.70, which
prevents the outgrowth of organisms. Fungi are a concern,
however, if product is not stored properly and aw is compro-
mised. Spoilage of cereal products can also occur through
blending with other, more spoilage‐prone ingredients during
processing.
Processing and Harvest
The main spoilage organisms of concern in improperly stored
cereal products are fungi—Aspergillus, Penicillium, and
Eurotium—which grow well on moist cereal grains and con-
taminate product after harvest. These organisms can be con-
trolled by maintaining water activity below 0.68, keeping
moisture low (under 11 percent for long‐term storage), and
storing at cool temperatures (41 to 77°F [5 to 25°C]) and in
controlled atmosphere (high carbon dioxide).
The moisture levels in flour, starch, and meal are crucial in
preventing spoilage. Below 12 percent moisture, flour is sta-
ble, but some molds can grow just above 12 percent, and
some bacteria can grow at 17 percent.
Dough‐Based Products
Most doughs are refrigerated, which significantly slows or
prevents spoilage. In addition, dough is anaerobic, with low
water activity, and has carbon dioxide–producing yeast or
leavening agents. Thus, it is rare that spoilage takes place.
However, sourdough bread can spoil if the lactic acid–
producing bacteria are not properly controlled. Visible results
of this type of spoilage include exploding packages, gas pro-
duction, and slimy dough.
Bread has a short shelf life, and the most common cause of
spoilage in baked bread is mold (such as Penicillium spp.) and
spore‐forming bacteria such as Bacillus subtilis, which sur-
vive the baking process and cause rope spoilage by breaking
down bread structure. Rope spoilage is identified when bread
develops a soft and sticky brown crumb with overripe canta-
loupe or pineapple odors. Bread spoilage can be prevented by
keeping products dry, lowering the water activity, using pre-
servatives such as sorbic acid and calcium propionate, and
employing good sanitation practices.
Dry pasta spoilage rarely occurs unless the product is
improperly dried or is contaminated during manufacturing
with spoiled ingredients such as bacterially contaminated
liquid eggs. This can be prevented by using pasteurized liquid
eggs. However, fresh pasta (with aw in excess of 0.9) is subject
c13.indd 281 1/14/2016 7:34:01 AM
282 • CULINOLOGY®
to microbial attack unless it is packaged using modified
atmosphere.
Nuts and Legumes
Nuts are “dry, one‐seeded fruit, which do not dehisce (split
open) at maturity.”42 Most true nuts are tree nuts; peanuts (also
called groundnuts) are technically in the legume family. Tree
nuts include almonds, hazelnuts, pistachios, Brazil nuts, pecans,
coconuts, and macadamia nuts. They can be minimally
processed and sold as a snack food, or they can be further
processed into butters or sauces, or used for oil production.
Tree nuts and peanuts rarely spoil. While their shells
protect them from microorganisms during growth, excessive
rain during harvest makes is possible for the shells to split
and fungi to enter and grow on the nut meat. Additionally,
inadequate post‐harvest drying and storage can result in
spoilage. The high fat content in nuts and seeds makes them
susceptible to attack by the fungi Aspergillus, Fusarium, and
Penicillium, which cause spoilage. Aspergillus can produce a
deadly toxin (aflatoxin).
Legumes
While fresh legumes typically have high levels of fungi on
their external pods, which cause spoilage such as black blight
and soft rot, the spoilage of dried legumes (for example, soy-
beans, chickpeas, black beans, lentils) is not common.
Legumes may be discolored by fungi, but this is not consid-
ered significant.
Eggs and Egg
Products
Eggs are sold whole in the shell, as a pasteurized liquid, or in
a spray‐dried powder form. Whole eggs are typically sold in
the retail and foodservice markets, while the liquid and pow-
der versions are more common in manufacturing operations.
All eggs and egg products have the potential to spoil if not
handled properly.
Shell Eggs
The microorganisms most common on egg surfaces are not
usually associated with spoilage. A few are able to penetrate
the eggshell. Pseudomonas can cause spoilage even in refrig-
erated eggs because that organism grows well under cold
conditions. Alcaligenes, Aeromonas, and Proteus cause black
rot because they are proteolytic and digest the egg albumen,
causing a blackening of the yolk. Shell egg spoilage can be
inhibited by good henhouse sanitation practices, frequent
egg collecting, refrigeration, regular egg inspections with
white lights (candling), and washing (and promptly drying)
eggs. While egg refrigeration is debated, it does indeed slow
the growth of all microorganisms.
In addition, one company currently markets a pasteurized
shell egg, “Davidson’s Safest Choice," that eliminates Salmonella,
and also reduces or eliminates spoilage organisms as well.
Following pasteurization, the eggs are wax‐coated to prevent
any contaminants from entering the egg through the porous
shell, and are marked with a red “P” in a circle to distinguish
them from regular, unpasteurized eggs. While they are more
expensive than regular shell eggs, they are much safer for
consumption by at‐risk populations such as the elderly or
immuno‐compromised individuals. Unlike regular eggs, the
USDA has stated: “In‐shell pasteurized eggs may be used
safely without cooking.”
Liquid Eggs
Liquid egg spoilage is a direct result of cross‐contamination
from the eggshell during processing. Liquid eggs must be pas-
teurized immediately or chilled or frozen as soon as possible
to prevent spoilage. Those that are pasteurized and salted or
sugared have a very long shelf life because pasteurization
destroys Pseudomonas, Acinetobacter, and Enterobacter,
and salt/sugar provide osmotic protection. However, if the
product is not pasteurized properly, halotolerant organisms
can survive and grow.
Spoilage in liquid eggs is evidenced by coagulation, sour
odors, and gas production. Washing and sanitizing eggshells
before breaking, pasteurization, cold or frozen storage, and
the addition of salt (or sugar in liquid eggs destined for bakery
applications) prevents or significantly slows spoilage.
Dried Eggs
Eggs that are spray‐dried, drum‐dried, or freeze‐dried are all
used in manufacturing and research and development. While
some bacteria can survive in dried eggs, they do not grow or
cause spoilage. When the product is reconstituted, any spoil-
age organisms present, even at low levels, can grow out and
cause the product to spoil if it is not subsequently heat‐
processed as an ingredient in another product.
Milk and Dairy
Products
“Milk is spoiled when it starts to look like yogurt, yogurt is
spoiled when it starts to look like cottage cheese, cottage
cheese is spoiled when it starts to look like regular cheese.
Regular cheese is nothing but spoiled milk anyway and can-
not get any more spoiled than it is already.”43
Milk in the U.S. is obtained almost entirely from cows
(97%) and to a much lesser extent from goats, whose milk is
primarily used for making cheese. The dairy products made
from milk include cream, evaporated or condensed milk,
dried milk, fermented milk (yogurt and kefir), cheese, butter,
and ice cream.
Milk is extremely susceptible to spoilage. With its high
water content, neutral pH, protein, fat, carbohydrates, vita-
mins, and minerals, it is an ideal growth environment for
spoilage microorganisms. Milk processing may include
pasteurization, sterilization, dehydration, fermentation,
c13.indd 282 1/14/2016 7:34:02 AM
CHAPTER 13 • Food Safety and Spoilage • 283
refrigeration, and freezing, all of which inhibit microbial out-
growth and extend shelf life.
Pasteurized Milk and Cream
Raw milk is not considered safe to drink by the FDA, and
almost all milk manufactured for consumption is pasteurized.
Milk that is pasteurized and refrigerated does not spoil for sev-
eral weeks, but eventually, spore‐formers (Bacillus spp.) and
thermoduric organisms (Streptococcus and Lactobacillus)
that survive the pasteurization process, or post‐pasteurization
contaminating bacteria, slowly begin to grow, even at refriger-
ated temperatures. As Streptococcus grows in milk, it uses the
lactose to produce lactic acid, which subsequently lowers the
pH of milk, allowing curdling to take place. Post‐processing
contamination can also result in fungal growth, which can
increase the surface pH of the milk, allowing spoilage bacteria
to grow.44 Pseudomonas spp. produce enzymes that break
down the fat (lipase) and protein (protease), which results in
off flavors and rancidity.
Shelf‐Stable Milk
Shelf‐stable milk is also known as UHT milk. In the regular
pasteurization process, milk is heated to 161.6°F (72°C) for at
least 15 minutes, whereas the UHT process heats the milk at
275°F (135°C) for one to two seconds. This flash of extreme
heat will kill off any bacteria and spores in the milk, making it
sterile and shelf stable at room temperature if packaged asep-
tically (i.e., packaged in a sterile container within the con-
fines of a “clean room”). Because of the UHT process and
aseptic packaging, shelf‐stable milk remains potable for
months, not days. Once the container is opened, however, it
reacts like regular milk and must be refrigerated. UHT pas-
teurization is also used for other products such as fruit juices,
cream, yogurt, wine, and soups.
When milk is sterilized to eliminate all microorganisms
and spore‐formers, spoilage is rare. When it does occur, it
usually is a result of post‐process contamination through a
breach in the packaging, or via the addition of ingredients
such as chocolate or other flavorings that introduce heat‐
resistant spores into the milk mixture.
One downside to UHT processing is that it adds an un -
avoidable cooked flavor to the milk. Product developers need
to take this into account when deciding whether to use steri-
lized or regular milk as an ingredient.
Concentrated Milk Products
Milk products are concentrated (for example, condensed
milk and evaporated milk) for direct consumption or to help
reduce shipping costs to manufacturing facilities that are
using them as ingredients in other products. Spoilage in con-
centrated milk products is similar to that seen in pasteurized
milk and is based on the number of heat‐resistant spores
(Bacillus spp.) that survive canning, or from post‐processing
contamination. Sweetened condensed milk, with a lower aw
(0.85), favors yeast and mold outgrowth, which can lead to off
flavors and gas production (exploding cans).
Dried Dairy Products
Spoilage organisms may be present at low levels in dried milk,
but no outgrowth or spoilage occurs until it is reconstituted.
Butter
Even though butter is very low in carbohydrate and protein, it
can be spoiled by bacteria and mold. Salted butter is rarely
affected; unsalted or “sweet” butter lacks the antimicrobial
benefit of added salt and is therefore far more likely to be
compromised. Sweet butter should always be stored refriger-
ated. There are two types of butter spoilage:
1. Surface taint (putridity), caused by Pseudomonas putrefa-
ciens, which grows on the surface of finished butter.
2. Rancidity, caused by Pseudomonas fragi, which hydrolyzes
the butterfat.
Other spoilage microorganisms include Lactobacillus lac-
tis, which causes a malty flavor, and Pseudomonas nigrificans,
which causes black discoloration.
Fermented Milk
Fermentation of dairy products is actually a very effective
method for preventing spoilage and extending shelf life. That
said, if the milk used is not clean and contains high levels of
other non‐fermenting bacteria, the contaminants can inhibit
the fermentation activity of the starter cultures. If the acidifi-
cation process is slow, spore‐formers, which do not grow at
low pH, can multiply before the product gets below pH 4.6.
Acid‐tolerant yeast and molds are common in fermented
dairy products and usually come from contaminated packag-
ing materials, the addition of contaminated fruit variegates,
or poor line hygiene. All of this leads to post‐process
contamination.
Cheese is essentially the conversion of milk into a less per-
ishable product. Over 1000 types of cheeses are made
throughout the world. The manufacturing steps include
heating, acidification (via fermentative bacteria or the addi-
tion of acids), enzymatic protein coagulation with rennet,
curd formation, whey removal, pressing, salting, and ripen-
ing. Spoilage microorganisms and undesirable molds, includ-
ing strains of Penicillium, Mucor, Monilia, and Aspergillus,
can be introduced at any point during the numerous process-
ing steps, resulting in inhibition of the specific molds and fer-
mentative bacteria needed to convert the milk into cheese.
Spoilage that takes place in fresh cheese immediately after
ripening is caused by Bacillus subtilis and coliforms. These
organisms can ferment lactose and cause early blowing (due
to gas formation), off odors, and slits in the cheese. Late‐
blowing spoilage is caused by the formation of butyric acid,
gas, and off flavors, and is caused by Clostridium spp. Spores
of these bacteria are capable of surviving pasteurization tem-
peratures and can be present in the milk.
To prevent cheese from spoiling, preservatives such as
potassium sorbate and lactic acid can be used to create an
unfavorable environment that inhibits spoilage bacteria.
c13.indd 283 1/14/2016 7:34:02 AM
284 • CULINOLOGY®
Starting with high‐quality milk is crucial, as are adequate
heat treatments, pure starter cultures of proven fermentative
bacteria to inoculate the milk, and proper sanitation proce-
dures to prevent contamination.
Oil‐Based Emulsified
Dressings
This category includes mayonnaise and salad dressings. Both
are oil‐and‐water emulsions that contain additional ingredi-
ents such as acidifying agents, colors, flavors, egg products,
sugar, salt, herbs, and other ingredients.
Commercial mayonnaise and spoonable (mayonnaise‐
like) salad dressings are formulated with acidulents, such as
vinegar and lemon juice, which create a high‐acid environ-
ment, and pasteurized eggs. Salt is also an important ingredi-
ent that contributes to the unfavorable environment for
microbial growth, making these products extremely unlikely
sources for bacterial spoilage.
The process involved in manufacturing oil‐based salad
dressings is usually cold fill, as heating the product would
destroy the emulsion. Thus, measures such as acidification,
addition of salt, and the addition of preservatives (sorbate,
acetic acid, and benzoate) are used to prevent spoilage.
Typical spoilage organisms that affect oil‐based products
include acid‐tolerant yeasts such as Zygosaccharomyces bailii
and fermentative bacteria such as Lactobacillus fructivorans.
The outgrowth of spoilage Lactobacillus in oil‐ and fat‐based
emulsified foods results in gas formation and a lowering of
the pH, which can impart an undesirable sour flavor in the
finished product.
Sugar, Syrups,
and Honey
This group of foods includes regular sugar, which comes from
sugar beets and sugar cane; sugar syrups, including liquid
sucrose, fructose, invert sugar, and corn syrups; maple syrup;
agave syrup; molasses; and honey. All of these in their raw
form are in close proximity to soil and the environment,
which can cause contamination and spoilage. In cane sugar
manufacturing, the spoilage can be seen as souring and alco-
hol and acid production in the liquid precursors before crys-
tallization. In beet sugar manufacturing, acid‐producing
bacteria such as Leuconostoc spp. can hydrolyze the sugar
and synthesize dextran, a glucose polymer, which looks like
slime. Dextran can clog the lines and pipes in sugar manufac-
turing facilities.
Maple sap is sterile when it is in the tree, but it can be
contaminated during tapping by Pseudomonas or yeasts.
These can cause flavor and color changes or result in blown
containers post‐packaging. Water activity can vary in honey
and molasses, depending on origin and climate. If the aw or
moisture levels are too high, osmophilic yeasts can grow and
cause alcoholic fermentation. Concentrated sugar syrups and
honey rarely spoil because of their high sugar concentra tion
and low water content (below 18 percent). Potential spoilage
organisms are killed by osmotic pressure. That said, honey
can contain bacterial spores such as Clostridium botulinum,
which are unable to germinate within the honey itself, and are
typically not harmful to healthy adults. But they can germinate
in the undeveloped gastrointestinal tract of babies and
colonize the small intestine, causing infant botulism (also
known as “floppy baby syndrome”). Therefore, honey should
never be fed to children below the age of 1 year.
Conclusion
Food and food ingredients can be contaminated with any
number of naturally occurring or synthetic biological, chemi-
cal, and physical hazards. Research chefs and product develop-
ers share the responsibility for preparing creative menu items
that also protect consumers’ health. Personnel must know the
causes of foodborne illness as well as how to prevent, elimi-
nate, or reduce the risk of foodborne illness to an ALOP.
The application of HACCP is a science‐based method to
assure the safety of processed foods. Tasting alone cannot
determine safety, as many pathogens do not produce detect-
able warning signs. However, if the process used to prepare
food is controlled using HACCP, the food has the greatest
likelihood of being safe. Careful control of processing time
and temperature, judicious use of approved preservatives,
adjustment of pH and water activity, and protective packag-
ing can significantly improve shelf life and maintain product
quality as well as assure food safety.
Food spoilage is not a safety problem but rather a quality
issue. Unlike food contaminated with pathogenic microorgan-
isms, which can taste and look fine, food spoilage can be detected
by taste, smell, and sight. Food is spoiled when it is damaged or
degraded to the point of being unfit for consumption.
Food spoilage shortens shelf life and its acceptability for
sale. Foods such as meat, fish, and poultry; vegetables and
fruits; spices, cereals, nuts, and legumes; egg and dairy prod-
ucts; oil‐ and fat‐based products; and sugar syrups are
affected by a wide variety of microbiological spoilage factors,
some of them unique to specific foods. Numerous manufac-
turing, distribution, and storage strategies can be used to pre-
vent food from spoiling beyond the point of salability.
Both food safety and spoilage threats are ubiquitous and
must be controlled to make food safe and desirable for con-
sumption. When product development kitchens and food
establishments implement effective, validated strategies and
procedures to control hazards and spoilage factors associ-
ated with the growing, receiving and storing, preparation and
packaging, transport, and distribution of food products, the
risk to public health and company liability is reduced, and the
company’s reputation is preserved and enhanced.
1J. N. Sofos, “Challenges to Meat Safety in the 21st Century,” Meat Science 78,
no. 1–2 (2008): 3–13.
2CDC Briefing on the Burden of Foodborne Disease Illness, Press Release
Archive, Wednesday, December 15, 2010.
c13.indd 284 1/14/2016 7:34:02 AM
CHAPTER 13 • Food Safety and Spoilage • 285
3R. L. Scharff, “Health‐Related Costs from Foodborne Illness in the United
States,” Georgetown University, March 2010. For the Produce Safety
Project, Georgetown University, http://publichealth.lacounty.gov/eh/docs
/ReportPublication/HealthRelatedCostsFromFoodborneIllnessUS .
4CDC (Centers for Disease Control and Prevention), Outbreak Database
Search Tool 2009 (Atlanta, GA: 2009). Available at http://www
.cdc.gov/foodborneoutbreaks/Default.aspx. Accessed September 24, 2009.
5CDC (Centers for Disease Control and Prevention), “CDC Fact Sheet:
Toxoplasmosis” (2008). Available at http://www.cdc.gov/toxoplasmosis/.
Accessed September 24, 2009.
6FDA (U.S. Food and Drug Administration), Bad Bug Book: Foodborne
Pathogenic Microorganisms and Natural Toxins Handbook (Washington,
D.C.: Center for Food Safety and Applied Nutrition, 2009). Available at
http://vm.cfsan.fda.gov/~mow/intro.html. Accessed September 29, 2009.
7D. L. Heymann, Control of Communicable Diseases Manual, 18th ed.
(Washington, D.C.: American Public Health Association, 2004).
8J. P. Smith, D. P. Daifas, W. El-Khoury, and J. W. Austin, “Microbial Safety of
Bakery Products,” in P. R. Ashurst, ed., Microbial Safety of Minimally Processed
Foods (Boca Raton, FL: CRC, 2003), 3–33.
9J. P. Smith, D. P. Daifas, W. El-Khoury, and J. Koukoutsis, “Foodborne Illnesses
Associated with Bakery Products,” Technical Bulletin 24, no. 4 (2002): 1–11.
American Institute of Baking.
10http://www.fsis.usda.gov/wps/portal/fsis/topics/regulatory‐compliance
/haccp/pr‐and‐haccp‐guidance‐documents.
11FDA, Defects Level Handbook. http://www.fda.gov/Food/GuidanceRegulation
/GuidanceDocumentsRegulatoryInformation/SanitationTransportation
/ucm056174.htm.
12J. M. Jay, M. J. Loessner, and D. A. Golden, “Food Protection with High
Temperatures, and Characteristics of Thermophilic Microorganisms,” in
Modern Food Microbiology, 7th ed. (New York: Springer Science and
Business Media, 2005).
13U.S. Food and Drug Administration, Food Code (Washington, DC: U.S.
Public Health Service, U.S. Department of Health and Human Services,
2009), retrieved March 30, 2010, from http://www.fda.gov/Food
/FoodSafety/RetailFoodProtection/FoodCode/FoodCode2009/.
14“Vital signs: Listeria Illness, Deaths, and Outbreaks: United States, 2009–
2011,” Morbidity and Mortality Weekly Report 62, no. 22 ( June 7, 2013):
448–452.
15FDA, Food Code.
16J. Hewitt and G. E. Greening, “Effect of Heat Treatment on Hepatitis A Virus
and Norovirus in New Zealand Greenshell Mussels (Perna canaliculus) by
Quantitative Real‐Time Reverse Transcription PCR and Cell Culture,”
Journal of Food Protection 69, no. 9 (2006): 2217–2223; Institute of
Food Science and Technology, “Foodborne Viral Infections,” 2001
retrieved September 10, 2009, from http://foodsafety.ksu.edu/articles/389
/foodborne_viral_infections .
17U.S. Department of Agriculture, “Draft Compliance Guidelines for Ready‐
to‐Eat Meat and Poultry Products,” 2001, retrieved September 1, 2009, from
http://www.fsis.usda.gov/OPPDE/rdad/FRPubs/97‐013P/RTEGuide .
18FDA, Food Code.
19USDA, “Draft Compliance Guidelines.”
20FDA, Food Code.
21USDA, “Draft Compliance Guidelines.”
22USDA, “Compliance Guidelines for Cooling Heat‐Treated Meat and Poultry
Products (Stabilization).” http://www.fsis.usda.gov/OPPDE/rdad/FRPubs
/95‐033F/95‐033F_Appendix%20B.htm
23FDA, Food Code.
24USDA, “Compliance Guidelines for Cooling Heat‐Treated Meat and Poultry
Products (Stabilization).”
25FDA, Food Code.
26FDA, “Draft Compliance Guidelines for Ready‐to‐Eat Meat and Poultry.”
http://www.fsis.usda.gov/OPPDE/rdad/FRPubs/97‐013P/RTEGuide .
27N. N. Potter and J. H. Hotchkiss, Food Science, 5th ed. (New York: Chapman
and Hall, 1999), 114.
28J. M. Jay, M. J. Loessner, and D. A. Golden, “Vegetable and Fruit Products,” in
Modern Food Microbiology, 7th ed. (New York: Springer Science and
Business Media, 2005), 125.
29J. M. Jay, M. J. Loessner, and D. A. Golden, “Intrinsic and Extrinsic Parameters
of Foods That Affect Microbial Growth,” in Modern Food Microbiology, 7th
ed. (New York: Springer Science and Business Media, 2005), 39–55.
30J. Farkas, “Physical Methods of Food Preservation,” in M. P. Doyle and L. R.
Beuchat (eds.), Food Microbiology: Fundamentals and Frontiers, 3rd ed.
(Washington, DC: ASM Press, 2007), 686–687.
31Ibid.
32Ibid.
33W. C. Frazier and D. C. Westhoff, Food Microbiology, 4th ed. (New York:
McGraw-Hill, 1988), 10.
34H. Kreuzer, “Research Reveals the Power of Dried Plums in Precooked
Meats,” Food Product Design, Supplement (February 2001), retrieved
November 10, 2009, from http://www.stapleton‐spence.com/downloads
/fpd0201 .
35J. M. Jay, Modern Food Microbiology, 4th ed. (New York: Van Nostrand
Reinhold, 1992), 55, 187, 237.
36J. M. Jay, M. J. Loessner, and D. A. Golden, “Processed Meats and Seafoods,”
in Modern Food Microbiology, 7th ed. (New York: Springer Science and
Business Media, 2005), 188–119.
37T. J. Montville and K. R. Matthews, Food Microbiology: An Introduction,
2nd ed. (Washington, DC: ASM Press, 2008), 276, 281.
38U.S. Food and Drug Administration, “Processing Parameters Needed to Control
Pathogens in Cold‐Smoked Fish,” Safe Practices for Food Processes, June 2009.
39Montville and Matthews, Food Microbiology, 271–299.
40International Commission of Microbiological Specifications for Foods,
“Microorganisms in Foods,” in Microbial Ecology of Food Commodities,
2nd ed. (New York: Kluwer Academic/Plenum Publishers, 1998), 237.
41Jay, Loessner, and Golden, “Vegetable and Fruit Products,” 137.
42International Commission of Microbiological Specifications for Foods, “Nuts,
Oilseeds, and Dried Legumes,” in Microbial Ecology of Food Commodities, 2nd
ed. (New York: Kluwer Academic/Plenum Publishers, 1998), 440–466.
43Montville and Matthews, Food Microbiology, 276, 281.
44Jay, Modern Food Microbiology, 55, 187, 237.
c13.indd 285 1/14/2016 7:34:02 AM
http://publichealth.lacounty.gov/eh/docs/ReportPublication/HealthRelatedCostsFromFoodborneIllnessUS
http://www.cdc.gov/foodborneoutbreaks/Default.aspx
http://www.fsis.usda.gov/wps/portal/fsis/topics/regulatory-compliance/haccp/pr-and-haccp-guidance-documents
http://www.fda.gov/Food/GuidanceRegulation/GuidanceDocumentsRegulatoryInformation/SanitationTransportation/ucm056174.htm
http://www.fda.gov/Food/FoodSafety/RetailFoodProtection/FoodCode/FoodCode2009/
http://foodsafety.ksu.edu/articles/389/foodborne_viral_infections
http://www.fsis.usda.gov/OPPDE/rdad/FRPubs/95-033F/95-033F_Appendix%20B.htm
http://www.stapleton-spence.com/downloads/fpd0201
http://www.cdc.gov/toxoplasmosis
http://vm.cfsan.fda.gov/~mow/intro.html
http://www.fsis.usda.gov/OPPDE/rdad/FRPubs/97%E2%80%90013P/RTEGuide
http://www.fsis.usda.gov/OPPDE/rdad/FRPubs/97%E2%80%90013P/RTEGuide
286 • CULINOLOGY®
14
Shelf-Life Extension
What Is Shelf Life?
Shelf life is the length of time that food, drinks, and other per-
ishable items have before they are considered unsuitable for
sale or consumption. Reasons can include deterioration of
sensory quality, diminution of nutritional attributes, and/or
spoilage. Shelf life is influenced by exposure to oxygen, light or
heat, transmission of gases or moisture, mechanical damage,
and contamination by insects, vermin, or microorganisms.
The shelf life of prepared foods depends on four factors:
ingredients and formulation, processing, packaging, and stor-
age conditions. Change any one of these conditions and you
change the shelf life—for better or worse.
As U.S. consumers become more knowledgeable and
aware of what goes into their foods, the traditional ways of
ensuring shelf safety, such as adding large amounts of sugar
or salt to manage water activity, or adding a dose of chemical
preservatives, are no longer acceptable for many new prod-
ucts. While these tactics are still widely used to extend shelf
life for many foods, new consumer purchasing trends demand
cleaner‐label shelf‐life extension technologies. “Better living
through chemistry” has lost some of its luster.
Many Americans today prefer foods that are minimally
processed and that contain fewer preservatives, less salt and
sugar, and more fresh ingredients than were previously
accepted. However, extended shelf life and perceived fresh-
ness do not go hand in hand. Products that traditionally have
the longest shelf life—dried and canned foods—will never be
mistaken for fresh. That said, shelf-life extension isn ’ t just
about maximizing the amount of time a food manufacturer
or retailer can safely sell a product. In a more pragmatic
sense, it ’ s about what humans have been doing for thousands
of years: protecting their food supply and maximizing its
usefulness.
There are many aspects to shelf stability : flavor and color
retention, microbial safety, nutrient retention, inhibition of
lipid oxidation, and prevention of other chemical, micro-
bial, and enzymatic degradations. These are made ever
more difficult to achieve by the growing emphasis on fresh,
all‐natural, and organic products. This puts more pressure
on manufacturers to develop methods that rely less on food
chemicals and more on alternate methods of preservation.
Some degratory factors can be controlled with appropriate
packaging. For example, the opaque paperboard gable‐top
containers used for most milk packages block UV and fluo-
rescent lighting, which can cause photo‐oxidation , initiate
lipid rancidity, and destroy certain vitamins; traditional
clear glass bottles do not. Packaging with impermeable bar-
rier materials to prevent moisture or gas migration into or
out of the package extends the shelf life of many foods.
Vacuum packaging or modified atmosphere packaging to
exclude or reduce the amount of oxygen can also help
extend shelf life. Natural preservatives and antioxidants
may be incorporated into food (or packaging) to extend
shelf life by inhibiting microbial growth or the destructive
effects of oxidation.
In most regions, a “ best before ,” “ best used by ,” or fresh-
ness dating is required on packaged perishable foods. Most
Lead Author: Klaus Tenbergen, Ed.D., CMB, ASBPB, MCFE , Dean of Career Technical
Education and Economic Development , Columbia College , Sonora , CA
Priscila D. Santiago‐Mora , Food Industry Engineer Instituto Tecnológico y de Estudios
Superiores de Monterrey , Campus Querétaro , México
Dennis Ferris, Ph.D. , Professor, Department of Food Science and Nutrition , California State
University , Fresno
Contributor: Dominic Man, BSc, MSc, FIFST , Principal Lecturer, Department of Applied Science,
Faculty of Engineering, Science, and the Built Environment , London South Bank University
Contributor: Shirley VanGarde, Ph.D. , VanGarde Consulting
c14.indd 286 1/22/2016 1:15:36 PM
CHAPTER 14 • Shelf-Life Extension • 287
shelf-life labels or listed expiration dates are guidelines that
presume safe handling of products. Consumption prior to the
expiration date does not necessarily guarantee the safety of a
mishandled perishable food or beverage; similarly, a product
is not necessarily dangerous or inedible after the printed
expiration date. A product that has passed its shelf life may
still be safe, but quality is no longer guaranteed. In most food
stores, shelf life is maximized by rotating stock, which
involves moving products with the oldest sell‐by date to the
front of the shelf in the hope that shoppers choose them first.
This concept is also known as first in, first out (FIFO). This is
important, as stores can be fined for selling out‐of‐date prod-
ucts, and most must discard such unsold products as shrink,
or waste, leading to a loss of profit.
For some foods, the printed shelf life is an important
indicator of safe consumption. Examples include deli meats,
greens, berries, seafood, raw meat, and soft cheeses.1
Bacterial contaminants are ubiquitous, and non‐sterile
foods left unused too long often acquire substantial num-
bers of organisms and become spoiled or dangerous to eat,
leading to food poisoning. However, the printed shelf life
itself is not an absolute indicator of food safety. For exam-
ple, pasteurized milk can remain potable for several days
after its sell‐by date if it is refrigerated properly. That said,
potability does not mean palatability ; even though sour
milk is probably safe to drink, its taste and texture is not
usually preferred.
Purpose of Food Preservation
The goals of food preservation are to increase shelf life while
retaining consumer acceptance, sensory quality, nutrients,
and safety. A good food preservation method must keep food
safe, retain quality, retain nutrients, be an asset to the food
distribution chain, be technically feasible, and capitalize on
available energy resources.
Keeping Food Safe
Early food preservationists did not understand relationships
between microorganisms, spoilage, and illness. They used
trial and error in establishing preservation methods. New
processing technologies and natural antimicrobial agents
are now developed in laboratories with strict adherence to
scientific principles and widely accepted experimental
techniques. Operating with a sound plan of Hazard Analysis
and Criti cal Control Points (HACCP) has become a food
industry standard.
To ensure healthfulness and safety:
• Follow proven methodology.
• Use sanitized equipment in good working order.
• Monitor the food during growing and harvesting, prior
to and during manufacturing, and during shipping, sub-
sequent distribution, and storage at selling points.
• Evaluate food through end of life for microbial contami-
nation and quality deterioration.
Retaining Quality
Preserving food in a way that retains good sensory quality is
second in importance only to preserving food safety. Food
products with unacceptable appearance, odor, texture, or
flavor are rejected. If the quality of food is poor, it isn’t pur-
chased, the processor loses business, the food is not eaten,
and the nutrients it contains are wasted. The length of stor-
age time desired and the type of food being preserved influ-
ences the best method for preserving it. Low temperatures
are effective, but refrigeration and freezing are not appropri-
ate for all foods. Most fresh produce does not do well in the
freezer. Many preservation methods also change the food’s
quality characteristics; dried foods are different from
canned or frozen or pickled, and most certainly from fresh.
We trade fresh sensory characteristics for extended keeping
ability.
Retaining Nutrients
Preserved foods should contribute not only protein, carbohy-
drates, fats, and fiber but also vitamins and minerals. Nutrient
losses can occur during processing, so techniques to minimize
loss should be used whenever possible. Nutrient fortification
may be desirable to add back nutrients lost or destroyed during
processing. Often, vitamin losses that occur during extended
storage parallel sensory losses; this is an incentive to preserve
both. As nutrient loss often parallels quality loss, both goals can
often be achieved at the same time.2
Being an Asset to the Food Distribution Chain
Canning in glass jars makes some foods more marketable,
but there are increased shipping losses due to breakage and
decreased nutrients and sensory quality due to light‐sensitive
compounds in the food. Also, glass is heavier and thus more
expensive to transport than other packaging options.
Environmental concerns about reducing non‐reusable/recy-
clable and possibly toxic packaging and employing environ-
mentally sound manufacturing practices are also an issue. It
is important to factor in product and transportation costs,
perishability, carbon miles, marketing appeal, environmental
packaging concerns, and distribution logistics as critical
parts of the product development process.
Being Technically Feasible
High‐tech preservation processes such as aseptic processing
and packaging (packing sterilized foods aseptically into pre‐
sterilized containers), high‐pressure processing (HPP), and
cold pasteurization (irradiation) are not applicable for all
foods. Small food processors with restricted funds are limited
in their equipment and processing choices, and even some
large processors must consider the impact (cost, space,
energy requirements, and so on) of new equipment. Advanced
processing research has discovered many innovative meth-
ods to improve shelf life, but some techniques cannot yet be
engineered economically for commercial use. Additionally,
local state and federal laws must be considered.
c14.indd 287 1/14/2016 7:34:58 AM
288 • CULINOLOGY®
Capitalizing on Available
Energy Resources
Dehydration is an excellent preservation method in hot, dry
climates, but it is more difficult to achieve in humid ones.
Freezing requires energy to maintain products at 0°F
(–18°C) throughout the distribution and storage period and
typically is not generally available in third‐world countries.
Thermally processed, commercially sterile (canned) foods
do not require refrigeration for storage, but their initial proc-
essing uses more water and heat energy than do drying or
fermentation. The cost of the raw ingredients is only a small
part of the actual cost of the finished product; processing
costs (and labor) must be factored in before development
begins.
Shelf-Life Labeling
Manufacturers’ tracking codes, though used on packaging for
decades, are a continuing source of confusion for consumers
who want to know the true age of products. Open shelf‐life
dating is the industry’s response. This process uses com-
monly recognizable terms such as day, month, and year to
indicate when the food was packaged or by when it should be
sold or used. Such dating is considered by most people a
measure of food freshness. It informs the buyer about the
interval between packing and purchase or use, and about the
relative freshness (and perceived quality) of the product as
well. However, the interval between packing and final con-
sumption is not necessarily the only factor leading to quality
loss; mishandling can occur at many stages during distribu-
tion. Therefore, an open date is not an absolute guarantee of
freshness—but it does serve as an indicator.
Open shelf‐life dating varies widely by product, state or
region, and manufacturer. Common open shelf‐life dates
include a sell‐by date, a best‐used‐by date, or a better‐used‐
by date. These dates help consumers decide how long the
product may be stored after purchase and assist grocery
stores with stock rotation.3
Best‐before and best‐used‐by dates indicate the date the
manufacturer intends the food to be consumed by for best
quality. That said, if the item was stored properly, the food
should still be safe for some period beyond that date. Sell‐by
dates are for stock rotation; they tell the store how long to
display products for sale. Use‐by dates are for perishable
(usually refrigerated) products; microorganisms may be a
concern past these dates. If the perishable food is frozen,
these dates do not apply during the frozen storage period.
Some foods have specific shelf‐life labeling regulations.
Because infant formula may be the sole source of nutrients
for babies, quality is critical. Infant formula must contain the
amount of nutrients stated on the label, and baby formula
must pass through a bottle nipple easily within the printed
date. Eggs have a U.S. Department of Agriculture (USDA)
pack date as a three‐digit code. It’s a Julian date; 001 is
January 1, and 365 is December 31. The sell‐by date on eggs
may not exceed 45 days from the pack date.4
Changes in Foods
During Storage
Foods change during storage due to the actions of enzymes,
microorganisms, chemical changes, and physical changes.
Some of these affect quality, some nutritional content, and
some the food’s safety (acute illnesses, such as bacterial infec-
tions or intoxications, and chronic concerns, such as cancers
and hypertension, which may be initiated or exacerbated due
to chemical changes). Preventing or slowing these changes
extends shelf life and product safety. This is usually done by
putting a series of hurdles in the way of deterioration. A hurdle
is a single preservation technique used to achieve one level of
food safety. Hurdle technology (also called combination preser-
vation or barrier technology) is the use of a series of hurdles,
each specifically targeted to one degratory component among
the cascade that any given food product may face. The hurdles
may include a cooking step, a packaging material, a preserva-
tive, or any ingredient, additive, or process that inhibits deteri-
oration and extends shelf life. For instance, jam is produced by
heating, adjusting pH, lowering water activity, and storing
under anaerobic conditions. Each of these preservation tech-
niques is a hurdle that a microorganism must get past in order
to grow. By combining hurdles, the intensity of each individual
preservation technique can be kept low to maintain quality
while working synergistically to provide protection. Because
foods are complex systems and the microorganisms and
enzymes that can affect them all have different weaknesses, an
array of preservation methods has a better chance of inhibiting
multiple offenders than any single treatment.
Biological Changes
Enzymes
Enzymes are proteins that catalyze specific reactions. They
may require a coenzyme (vitamin) or a cofactor (mineral) to be
activated. Enzymes occur in all plants, animals, and microor-
ganisms, and they are present in every food. Some are also
added during food processing for their specific synthesizing,
degrading, or interconverting actions as dough relaxers, juice
clarifiers, and meat tenderizers, for example. Enzyme common
names have the suffix ‐ase on the substrate name—for exam-
ple, lipase, protease, cellulase, lactase, thiaminase. Enzyme
nomenclature can also describe specific action—for example,
oxidase, dehydrogenase, synthase.5
Some enzyme activity improves products during storage:
fruits picked green ripen later by the effect of polygalacturonase
and pectinesterase during transportation6; mixing invertase
with fondant hydrolizes the sucrose in the fondant, creating a
liquid center in filled candies (such as chocolate cherries)7; add-
ing alpha‐amylase to bread dough lowers the rate of amylopec-
tin retrogradation, slowing the staling process8; and aging of
meats and cheeses is brought about through the action of pro-
teases and lipases.9 However, enzyme activity in stored foods
can shorten shelf life by altering flavor, color, texture, and
nutrients, especially the loss of unstable or labile vitamins.10
Enzyme activity, although it is slowed, occurs even during frozen
c14.indd 288 1/14/2016 7:34:59 AM
CHAPTER 14 • Shelf-Life Extension • 289
storage. This is evident in the development of rancidity in fatty
foods frozen for months. Enzyme activity is inhibited by
in activating or denaturing the enzyme, removing cofactors or
coenzymes required for activity, decreasing substrates or
intermediate products, or altering the chemistry of the sur-
rounding environment (including the level of oxygen in the sur-
rounding atmosphere). Enzymes are proteins; their activity can
be destroyed by exposure to extremes of heating or pH.
FIGURE 14.1 Enzymes naturally present in foods that can degrade quality during storage.
Enzyme Degradation Product Preventive Action
Polyphenyl oxidase (PPO)
Converts phenolics into o‐quinones;
brown colors, vitamin C loss.
Darkens produce;
creates dark spots
on crustaceans.
Requires Cu+2, oxygen; cells break to
mix enzyme and substrate.
Control: Thermal inactivation.
Keep cells intact (slow respiration).
Use antibrowning agents (sulfites, cysteine,
glutathione).
Ascorbic acid decreases o‐quinone
formation, removes some brown comp.
Acids decrease pH below PPO activity
(oxalio>oxalacetic>malonic>tartaric>
pyruvic>citric>malic>lactic).
Halide salts (NaCl, CaCl
2
) alter ionic
environment, inhibit PPO and chelate
Cu ions.
Sugars (especially glucose and fructose)
decrease aw and inhibit PPO.
Maillard reaction products (glu‐cysteine and
fru‐cysteine) inhibit PPO.
Proteolytic enzymes (ficin, bromelain,
papain) destroy PPO.
Other, often used in combination with the
above: EDTA binds Cu, pineapple juice,
honey, ascorbate, irradiation, UHP.
Pectin methyl esterase (PME) and
polygalacturonase (PG)
PME converts pectin to galacturonic acid
and methanol.
Galacturonic acid cleaved by PG.
Pectin lyases, cellulases, and xylanases.
Cleave pectin molecule.
Softening
as pectin molecule
is cleaved.
Thermal inactivation.
UHP inactivation.
Alpha‐farnesene
Catalyzes oxidation after natural
antioxidants degraded during long storage.
Scald in apples. Postharvest dip diphenylamine.
Ethoxyquin wax or wraps.
Lipoxygenase (LOX)
Oxidation of unsaturated lipids to
H2O2 and free fatty acids (f.f.a.).
Off flavors and odors;
destroys carotenoids,
vitamin C, thiamine;
bleaches chlorophyll;
produces free radicals.
LOX contains Fe atom so metal
scavengers inhibit.
Needs oxygen, limit it.
Heat treatments may inactivate
(depends on food).
Peroxidases
Generate free radicals.
Can form lignin.
Ascorbic acid oxidase degrades ascorbic
acid and brown compounds can result.
Degrades flavor and aroma,
vitamins.
Toughens produce.
Peroxidases are heat labile, and low pH
inhibits (range varies).
Alpha amalase
Converts starch into sugars.
If active after baking,
creates gummy crumb.
Heat labile.
Trimethylamine demethylase
Cross‐links proteins.
Hardens fish during
frozen storage and thawing.
Lower-temperature storage, lower pH.
Proteolytic enzymes
(transaminase) Releases glutamic acid;
breaks proteins releasing f.f.a.
Softens meat tissue;
develops flavor and odor
compounds.
Lower temperature to stop aging process.
Sources: Miyawaki (2006);11 Robinson (2001);12 Wills (1989);13 Anese (2006);14 Si and Dorst‐Lustenberger (2002).15
c14.indd 289 1/14/2016 7:34:59 AM
290 • CULINOLOGY®
Microorganisms
The prime biological factors involved in food deterioration
are microorganisms (bacteria, yeasts, and molds).
To increase the shelf life of more perishable foods (such as
low‐sugar jam, low‐salt condiments, low‐oil salad dressings,
prepared fresh produce, and deli meats), food companies
must take these steps:
1. Reduce the intrinsic microbial load (bacteria, yeast, mold)
from all raw materials used before processing them into
the finished food.
2. Process the finished food so as to reduce the microbial
population (both vegetative organisms and spores).
3. Package the food so as to prevent recontamination and
protect the contents during the trip from manufacturer to
market.
Microorganisms have specific temperature, pH, oxygen,
and moisture ranges where they function best. Shelf‐life
extension strategies inactivate microbes or adjust the food
environment out of those preferred growth ranges (or to the
edge of the range, and then add other hurdles). Common
antimicrobial hurdles used in foods are:
• pH—Keep below 4.6 to inhibit pathogens. This is accom-
plished by adding food‐grade acids or by creating an
acid environment in situ via fermentation.
• Moisture—Keep below aw 0.90 for most bacteria; below
0.88 for most yeasts; below 0.86 for pathogenic
Staphylococcus aureus; below 0.80 for most molds.16
Lowering the moisture level (or, more appropriately,
decreasing the accessibility of the water that is pres ent)
creates a hostile environment for microorganisms
by decreasing the medium they need to grow in.
Depending on the method used (for example, decreas-
ing water activity through the addition of increased
solutes such as salt or sugar), even the water itself can
become a weapon: The increased osmotic pressure on
the bacteria causes them to burst. Although adding
salt or sugar is a traditional, time‐tested way to control
water activity to prevent bacterial growth, modern
product developers don’t usually have the freedom to
feature lots of salt or sugar on product labels. Instead,
water activity can be reduced in fruit‐only jams and jel-
lies by starting with fruit concentrates (prepared by
removing water in the first place) and then preparing
under vacuum to allow lower-temperature processing.
Similar techniques are used in other reduced‐sugar
foods. This provides the desired osmotic effect without
adding sugar.
• Gas requirements—Manipulate (add, remove, modify)
oxygen and carbon dioxide levels to inhibit microbial
respiration and reproduction.
• Temperature—To prevent outgrowth, keep foods above
or below the danger range (40 to 140°F [4.4 to 60°C])—
that is, the range at which pathogenic bacteria grow.
That said, listeria and C. botulinum types E, B, and F can
grow below 40°F (4.4°C),17 so if these are a potential
danger, additional inhibiting hurdles must be used (anti-
microbial agents, acidification, modified atmosphere
packaging, and so on). Most bacterial growth stops at
10 to 16°F (–12 to –9°C).18
• Other hurdles—Plan for nutrient limitation, antimicro-
bial chemicals, competing safe organisms, and so on.
Fungi
Fungi are members of the plant kingdom that do not pos-
sess the usual roots, stems, and leaves, nor do they possess
the green photosynthetic pigment chlorophyll. Fungi
exhibit a wide range of forms, including mushrooms and
truffles, molds, and yeasts. Some food products require the
aid of fungi in their production. For example, yeast in baked
bread is necessary for making the dough rise and providing
flavor ; molds are invaluable in the aging of many highly
prized cheeses (Roquefort, Camembert, Stilton, Brie).
Fungi are valuable as a source of antibiotics, vitamins, and
various industrially important chemicals, such as alcohols,
acetone, and enzymes, as well as for their role in fermenta-
tion processes, as in the production of alcoholic beverages,
vinegar, cheese, and bread. At the same time, yeasts and
molds are responsible for an enormous amount of food
spoilage.
Molds
Molds are multicellular fungi that form a filamentous
branching growth known as a mycelium. The mycelium is
composed of individual filaments called hyphae. Molds are
aerobes: they require oxygen for growth, so some part of the
organism must be on or near the food’s surface, where oxy-
gen is accessible. In this case, the mold is usually character-
ized by its fuzzy or cottony appearance. In addition, parts of
the organism may also grow within the food, such as the
mold in blue cheese. Even so, holes are drilled into these
cheeses to allow greater access to oxygen for enhanced
mold growth.
Foods processed without preservatives are at high risk for
mold. Fruits and vegetables with high moisture content can
be contaminated through tiny fissures or bruises on the
surface, as can porous foods, such as breads and cakes. Spores
fall on the surface and germinate. They send roots (rhizoids)
into the food to absorb nutrients and send up stalks on which
new spores eventually form (what we see as the fuzzy stuff ).
By the time we see the surface fuzz, the mold has already
infiltrated the food. It’s more difficult for mold to penetrate
dense foods. Also, bacteria can grow alongside mold in soft
cheeses. A contaminated cutting instrument can spread
mold spores or bacteria to other foods.
Molds can be beneficial in making certain kinds of
cheeses and can develop either on the surface of cheese, or
internally, or both. Blue‐veined cheeses such as Roquefort,
blue, Gorgonzola, and Stilton are created by the introduction
c14.indd 290 1/14/2016 7:34:59 AM
CHAPTER 14 • Shelf-Life Extension • 291
of Penicillium roqueforti spores. Cheeses such as Brie and
Camembert have white surface molds (typically P. camem-
berti). Other cheeses have both an internal and a surface
mold. Hard salami and dry‐cured country hams may have
surface mold, which is normal for these shelf‐stable
products. The molds used to manufacture these foods are
safe to eat.
Yeasts
Yeasts are microscopic unicellular fungi; they do not form a
mycelium and instead exist as single cells. Yeast cells may
be round, egg‐shaped, or elongated. The majority reproduce
asexually by budding, but a few species reproduce by sim-
ple fission, like bacteria. The generation time for most
yeast cells is 20 to 30 minutes under ideal conditions.19
Yeasts produce metabolic end products, including acids,
carbon dioxide, and alcohols, that cause the physical,
chemical, and sensory properties of a food to change. In
some cases this leads to spoilage; in others it leads to desir-
able changes, as in breads and alcoholic beverages. The
growth of yeast on food products can be seen as a powdery
coating on their surface, as in cheeses or meats; as cloudi-
ness in otherwise transparent liquids, such as brines; and
by the fermentation of sugars in beverages, leading to off
flavors and carbon dioxide fizziness in juices, wine, and
semiliquid products such as syrups and jams. Yeasts of the
Zygosaccharomyces genus have a long history as spoilage
organisms within the food industry. This is because these
species can grow in the presence of high sucrose, ethanol,
acetic acid, sorbic acid, benzoic acid, and sulfur dioxide
concentrations, which are among the most commonly
used food preservatives.
Bacteria
The adaptability of bacteria, which enables them to live in
extreme environmental conditions, often causes unexpected
problems in the food industry.
Typically, bacteria measure 1 to 3 μm in length and 0.4 to
1 μm in width, although others larger and smaller than this
occur. Bacteria reproduce or multiply by a process called
binary fission, which involves the simple division of the cell
into two new identical daughter cells. Bacteria can be classi-
fied by their shape into spheres (cocci; singular: coccus), rods
(bacilli; singular: bacillus), and curved, comma-shaped, or
twisted rods (spirilla; singular: spirillum). Besides food spoil-
age, bacteria can cause foodborne illness, but fewer than
20 of the many thousands of different bacteria actually are
the culprits. More than 90 percent of food poisoning cases
each year are caused by Staphylococcus aureus, Salmonella
spp., Clostridium perfringens, Clostridium botulinum, Campylo-
bacter jejuni, Listeria monocytogenes, Vibrio parahaemolyticus,
Bacillus cereus, and enteropathogenic Escherichia coli such as
E. coli O157:H7. These bacteria are commonly found on many
raw foods, as many are normal constituents of soil and fecal
matter (manure‐based fertilizer). Illness and spoilage can be
prevented by:
1. Controlling the initial number of bacteria present by wash-
ing, sanitizing, peeling, hide removal, blanching, etc.
2. Preventing that small number from growing by using any
or all of the hurdle techniques mentioned previously.
3. Destroying the bacteria (and their spores) by proper
cooking.
4. Avoiding recontamination through appropriate packaging.
Because food‐poisoning and spoilage bacteria are often
present on many foods, knowing the characteristics and
morphology of each bacterium is essential to designing an
effective control program. (See Chapter 13 for a more com-
plete discussion of food safety.)
Chemical Changes
Chemical reactions in foods are not usually reversible because
they involve the formation of new compounds. Enzymatic
reactions, browning reactions, and oxidative reactions are
responsible for the majority of household discards, so their
effects and prevention are important to understand in the
food industry.20
Browning Reactions
Two important types of browning reactions occur in foods;
enzymatic browning and non‐enzymatic browning. The
changes from enzymatic browning are generally undesirable,
so precautions must be undertaken to prevent it. But the
colors and flavors developed via non‐enzymatic or Maillard
browning reactions are important and often (but not always)
desirable, so inhibiting or enhancing it is a case‐by‐case
decision.
Enzymatic Browning
Enzymes are specialized proteins that catalyze specific reac-
tions in foods. In fruits and vegetables, polyphenoloxidase is
primarily responsible for enzymatic browning. It oxidizes
FIGURE 14.2 Mycelium, the mass of hyphae
(slender, elongated, threadlike cells or filaments
of cells) forming the body of a fungus.
c14.indd 291 1/14/2016 7:34:59 AM
292 • CULINOLOGY®
FIGURE 14.3 Bacteria.
Bacteria
Responsible Description Typical Foods Cause
Temperature
Sensitivity
Staphylococcus
aureus
Produces a heat‐stable
toxin.
Meat and seafood
salads, sandwich
spreads, and high‐salt
foods.
Poor personal hygiene
and subsequent
temperature abuse.
No growth below 40°F.
Bacteria are destroyed
by normal cooking, but
toxin is heat stable.
Salmonella spp. Produces an intestinal
infection.
High-protein foods:
meat, poultry, fish,
and eggs.
Contamination of
ready‐to‐eat foods,
insufficient cooking,
and recontamination
of cooked foods.
No growth below 40°F.
Bacteria are destroyed
by normal cooking.
Clostridium
perfringens
Produces spores and
prefers low‐oxygen
atmosphere. Live cells
must be ingested.
Meat and poultry
dishes, sauces, and
gravies.
Improper temperature
control of hot foods,
and recontamination.
No growth below 40°F.
Bacteria are killed by
normal cooking, but
heat‐stable spores can
survive.
Clostridium
botulinum
Produces spores and
requires a low‐oxygen
atmosphere. Produces
a heat‐sensitive toxin.
Home‐canned foods. Improper methods
of home processing
foods.
Type E and Type B can
grow at 38°F. Bacteria
are destroyed by
cooking, and the toxin
is destroyed by boiling
for 5 to 10 minutes.
Heat‐resistant spores
can survive.
Vibrio
parahaemolyticus
Requires salt for
growth.
Raw and cooked
seafood.
Recontamination
of cooked foods or
eating raw seafood.
No growth below
40°F. Bacteria killed by
normal cooking.
Bacillus cereus Produces spores and
grows in normal‐oxygen
atmosphere.
Starchy food. Improper holding and
storage temperatures
after cooking.
No growth below
40°F. Bacteria killed by
normal cooking, but
heat‐resistant spores
can survive.
Listeria
monocytogenes
Survives adverse
conditions for long
periods.
Milk, soft cheeses,
vegetables fertilized
with manure.
Contaminated raw
products.
Grows at refrigeration
(38–40°F) temperatures.
May survive minimum
HTST pasturization
temperatures (161°F
for 15 seconds).
Campylobacter
jejuni
Oxygen sensitive,
does not grow above
116°F.
Meat, poultry, milk,
and mushrooms.
Improper pasteurization
or cooking, cross‐
contamination.
Sensitive to drying or
freezing. Survives in
milk and water at 39°F
for several weeks.
Yersinia
enterocolitica
Not frequent cause
of human infection.
Milk, tofu, and pork. Improper cooking.
Cross‐contamination.
Grows at refrigeration
temperatures (35–40°F).
Sensitive to heat
(122°F).
Enteropathogenic
E. coli
Can produce toxins
that are heat stable
and others that are
heat sensitive.
Meat and cheeses. Inadequate cooking.
Recontamination of
cooked product.
Organisms can
be controlled by
heating. Can grow
at refrigeration
temperatures.
c14.indd 292 1/14/2016 7:34:59 AM
CHAPTER 14 • Shelf-Life Extension • 293
phenolic compounds in fruits and vegetables to produce
severe discoloration. It is activated whenever certain fresh
fruits or vegetables are cut or bruised, allowing the enzyme to
escape from within normally intact cells and become exposed
to oxygen. Commonly affected produce include potatoes,
avocados, bananas, pears, and apples.
Blanching (brief immersion in scalding water) is one
method used to prevent enzymatic activity by heating to the
point of protein denaturation. Of course, this is not always
practical, especially for produce items usually eaten raw
(bananas, avocados, and so on). Alternative methods include
reducing pH by acidification (such as adding lime juice to
guacamole or squeezing lemon juice on cut apples), blocking
exposure to oxygen by vacuum‐packing or covering with cold
water, using antioxidants (such as ascorbic acid and sodium
bisulfite), and refrigeration, which slows but does not com-
pletely halt enzymatic activity or browning.
Non‐Enzymatic Browning
In France in 1912, Louis‐Camille Maillard first scientifically
described a browning reaction in a series of experiments
heating reducing sugars (typically monosaccharides: glu-
cose and fructose) together with amino acids. Because the
brown‐colored compounds are created without enzymes,
this reaction is also called non‐enzymatic browning. In a
series of reactions, a reducing sugar reacts with an amino
group (usually an amino acid or lysine residue of a protein)
and forms a variety of products that alter flavor, odor, color,
rehydration properties, and nutritional value.21 Unlike enzy-
matic browning, the Maillard reaction is not completely
coccus diplococci diplococci
encapsulated
Pneumococcus
Staphylococci
tetradsarcinastreptococci
coccobacillus bacillus
diplobacilli
Streptobacilli
Filamentous spirochete
Corkscrew’s form
Borrelia burgdorferi
Club Rod
Corynebacteriaceae
Vibrio
Comma’s form
B. dellovibrio
enlarged rod
Fusobacterium
Helical form
Helicobacter pylori
palisades
Cocci Others
Bacilli
Budding and appendaged bacteria
hypha
stalk
FIGURE 14.4 Bacterial morphology diagram.
Source: Wikipedia.
c14.indd 293 1/14/2016 7:35:00 AM
294 • CULINOLOGY®
negative; the browned color and flavor of baked cakes,
breads, cereals, roasted and grilled meats, chocolate, coffee,
and even caramels is due to this reaction between sugar and
protein. It does not require much of either reactant; orange
juice and canned pears have enough protein and beef has
enough sugar to support the reaction.
Protein aggregates are formed by the Maillard reaction
that may reduce the nutritional value of a product, as amino
acids can be lost, especially the basic amino acids: lysine,
arginine, and histidine. Sometimes the flavors developed
are not appreciated, such as the cooked flavor in sterilized
milk.
The Maillard reaction is favored by heat and time. Most food
is between pH 3 and 8, where the reaction progresses well.22
Moisture content is important; intermediate- moisture foods
have high rates of reaction. The sugar molecule itself affects the
rate of reaction: xylose > monosaccharide hexoses (galactose,
mannose, glucose, fructose) > disaccharides (maltose, lactose).23
Not being a reducing sugar, sucrose (table sugar) does not
participate in Maillard browning.
To control the Maillard reaction, the temperature can be
reduced, the pH lowered, the moisture adjusted to very high
or very low levels, one of the substrates removed (enzymes
can be used to remove sugar from eggs before drying), or
additives used, such as sulfur dioxide or sulfites, which
prevent brown compound formation but not protein
degradation.
The most common toxic compound as a Maillard reaction
product is acrylamide, which forms from sugars and an
amino acid (asparagine) during high-temperature cooking—
the baking, frying, or roasting of carbohydrate-based foods,
such as potato products, grain products, and coffee.
Acrylamide is not present in foods when boiling and steam-
ing foods; generally, it is more likely to accumulate when
cooking is done for longer periods or at higher temperatures.
In response to the enactment of Proposition 65 (the Safe
Drinking Water and Toxic Enforcement Act of 1986) in
California, numerous consumer products have been refor-
mulated to eliminate these toxic chemicals and re-labeled to
demonstrate their absence.
Lipid Oxidation
Lipid oxidation severely compromises the quality of some
foods and limits the shelf life of others. Oxidation of unsatu-
rated lipids (autoxidation) is a series of reactions in which
oxygen attacks unsaturated double bonds to produce
hydroperoxides and free radicals, which are highly reactive
and can produce both more free radicals (via chain reaction)
and short‐chain compounds with off flavors, odors, and
health concerns. Deleterious changes in foods caused by
lipid oxidation include loss of flavor, color, nutrient value
and functionality, development of rancid off flavors, and
accumulation of compounds that may be detrimental to
health. All foods that contain unsaturated lipids are
susceptible to oxidation; especially affected are foods
containing high levels of polyunsaturated fats as well as
those that are dehydrated, subjected to high temperatures,
or cooked and subsequently stored—for example, dehy-
drated eggs, cheeses, and meats; foods fried in unsaturated
oils; and cooked (uncured) meats. Oxidative rancidity is a
major shelf‐life limiter ; it proceeds even when microorgan-
ism growth is halted and even in products frozen for long
periods. Once a product (polyunsaturated oils or the foods
cooked in them, fatty meats, whole grains, and so on)
becomes rancid, it can never be cleansed.
The rate of lipid oxidation can be manipulated to reduce
or inhibit it by various means.
Temperature
Generally, the rate of oxidation increases as temperature
increases.
Surface Area
Large surface area results in more lipid‐oxygen contact.
Pro‐Oxidants
Metal ions promote lipid oxidation. Cobalt, copper, iron,
manganese, and nickel are widely present in foods, and virtu-
ally all food manufacturing facilities use equipment made
from metals. Strategies to reduce the effect of unavoidable
exposure to metals include the use of sequestering agents
that chelate (tie up) metals, making them unavailable to par-
ticipate in the oxidation reaction. EDTA (ethylenediamine-
tetraacetic acid) in mayonnaise is a common example.
Natural Antioxidants
Natural antioxidants are found in varying amounts in foods
such as vegetables, fruits, grain cereals, eggs, meat, legumes,
herbs, and nuts. Some antioxidants, such as lycopene and
ascorbic acid, can be destroyed by long‐term storage or pro-
longed cooking. Other antioxidant compounds are more sta-
ble, such as the polyphenolic antioxidants in whole wheat
cereals and tea. The effects of cooking and food processing
are complex, as these processes can also increase the bio-
availability of some antioxidants, such as some carotenoids
in vegetables. In general, processed foods contain fewer anti-
oxidants than fresh and uncooked foods, as the preparation
processes may expose the food to oxygen, thus reducing or
using up the antioxidants normally present.
Many herbs, including rosemary, oregano and sage, from
the Labiatae family, possess strong antioxidant activity. Anti-
oxidant phenolic compounds that have been isolated from
these herbs include carnosol from rosemary, carvacrol from
oregano, and carnosic acid from sage.
Rosemary extracts are widely used in meat processing,
including beef, pork, and poultry products, to retard the onset
of warmed‐over flavor due to lipid oxidation. Rosemary
extracts are available in many forms, including oil‐soluble,
water‐soluble, liquid, and powdered, and in deodorized
varieties that reduce the rosemary flavor. Used especially in
c14.indd 294 1/14/2016 7:35:00 AM
CHAPTER 14 • Shelf-Life Extension • 295
high fat‐containing deli meats and sausages, the improve-
ment in shelf life is fairly dramatic. Water‐soluble rosemary
extracts are easily dispersed in the brines used in manufac-
turing deli meats. Use levels as low as 0.05 to 0.1 percent help
delay the onset of oxidative rancidity. Either dry or liquid
rosemary extract is useful in extending the flavor and color of
ground beef patties from 8 days to 11 or more. It is added
before the meat is ground and protects lipids within the mus-
cle‐cell membranes, which are the most susceptible to lipid
oxidation, especially when grinding exposes these surfaces to
oxygen and to pro‐oxidants such as salt or iron‐containing
heme pigment in the meat.
Raisin and dried plum extracts can also impart antimicro-
bial properties to some food products at levels that do not
significantly affect sensory characteristics.
Ascorbic acid, or vitamin C, is used in the manufacture of
fruit juices and purees from fruits such as apples and peaches.
It is added during the crushing, straining, or pressing
processes to prevent enzymatic browning of the raw fruits.
Ascorbic acid is also widely used in the meat industry for its
antioxidant properties. In cured meats, ascorbic acid can
accelerate color development, inhibit nitrosamine formation
(in the United States, meats to which nitrites are added must
contain ascorbic acid as well), prevent oxidation, and prevent
color fading.
Natural mixed tocopherols (forms of vitamin E), consist-
ing of d‐alpha (α), d‐beta (β), d‐gamma (γ), and d‐delta (δ)
tocopherol, can help maintain the freshness and shelf life of
lipid‐containing products. They are a natural alternative to
synthetic antioxidants such as BHT (butylated hydroxytolu-
ene), BHA (butylated hydroxyanisole), and TBHQ (tertiary
butylhydroquinone). One benefit is that consumer‐friendly
label claims and ingredient legends declaring “Natural vita-
min E added to preserve freshness” or “Natural vitamin E
added to protect flavor” can be used.
Mixed tocopherols are resistant to high‐temperature food
processing and have low volatility and good solubility in
fats and oils. They have been tested in many applications,
including baked goods, cereals, dehydrated potatoes, nuts,
and fried noodles (ramen), and in foods such as meat and
egg products and tuna fillets. Their solubility in lipids makes
them useful in fats and oils such as vegetable and fish oils,
milkfat, poultry fat and lard, and in food ingredients such as
citrus oils or lecithin.
Other natural antioxidants, including resveratrol (from
grape skin extract) and pycnogenol (a phytochemical from
tree bark) show promise in university trials on ground beef
and poultry products. Both antioxidants are approved as
GRAS (generally recognized as safe) by the FDA. Still others,
including 4‐hexylresorcinol, ascorbic acid, and glutathione,
have been tested as possible replacements for sulfites, as
antimicrobial agents, and to prevent color changes in
dehydrated fruits and vegetables. Sulfites must always be
labeled if used at levels above 10 ppm because of their effect
on the considerable portion of the population that suffers
from asthma and other pulmonary inflammatory diseases.
Hexylresorcinol has also been tested on fruits and vegetables
and is also used to control blackspot in fresh and frozen
shrimp.
Specific, accurate measurements of antioxidants is essen-
tial, as is uniform blending with raw ingredients to ensure
the antioxidants are effective. Some natural antioxidants are
not heat stable, so they must be added during a cool‐down
phase of any heat process. Oil‐based antioxidants, such as
vitamin E, can be easily dispersed in small amounts of fats or
oils prior to blending with the food mass for more uniform
dispersion.
Synthetic Antioxidants
Synthetic antioxidants are widely used as food additives to
prevent rancidification, owing to their high performance, low
cost, and wide availability. Examples include propyl gallate
(PG), tertiary butylhydroquinone (TBHQ), butylated hydroxy-
anisole (BHA), and butylated hydroxytoluene (BHT). These
additives are used primarily to prevent rancidity in unsatu-
rated fats and oils. Of course, when these protected fats and
oils are used in the manufacture of other foods such as mar-
garine, mayonnaise, salad dressings, fried products, baked
products, sauces, dips, snack foods, and chicken soup mix,
the antioxidants are carried along with the fats into these
other foods, where they continue to provide shelf‐life exten-
sion benefits to the fats in the finished product and therefore
to the finished product itself. They save millions of dollars
worth of foods from going rancid and being discarded. In
every case, synthetics exhibit stronger antioxidant activity
than natural antioxidants, but they are obviously not as
label‐friendly.
Radiant Energy
Radiant energy is the energy from light. Light waves, visible
and invisible, accelerate oxidation. Both sunlight and fluo-
rescent lighting can initiate photo‐oxidation. Milk and
other lipid-containing products are susceptible to oxidative
deterioration from exposure to light from either natural or
artificial sources. Light catalyzes certain chemical reac-
tions, resulting in the development of off flavors and the
breakdown of pigments and vitamins. That is why the
majority of milk is packaged in opaque paperboard contain-
ers rather than glass bottles. Pigment destruction in cooked
green vegetables exposed to fluorescent lighting commonly
found in refrigerated display cases is quite rapid. Opaque
packaging and storage out of direct exposure to light can
inhibit this.
Hydrogenation
Both mono‐ and polyunsaturated fats can be easily oxidized,
leading to rancidity. Polyunsaturated fats have more reaction
sites (unsaturated double bonds) and are therefore more sus-
ceptible. Hydrogenation has been used for years as an effec-
tive process to reduce or eliminate unsaturated double bonds
in unsaturated oils in order to control their reactivity with
c14.indd 295 1/14/2016 7:35:00 AM
296 • CULINOLOGY®
oxygen and create more stable (and more functional) vegetable‐
based shortenings resistant to oxidative rancidity; these can
be substituted for cholesterol‐containing animal fats such as
lard and tallow. Unfortunately, in recent years, partially
hydrogenated fats have been implicated as the major source
of trans fats in the diet. Trans fats are strongly associated
with an assortment of diseases, including coronary heart
disease, diabetes, Alzheimer’s disease, cancer, and obesity,
among others. As such, since 2006, the FDA has mandated
that manufacturers list trans fat levels on the nutrition facts
panel of all foods.
Encapsulation
Encapsulation involves the entrapment of food ingredients,
enzymes, and flavors, or the elimination of exposure to oxy-
gen. Ap plications for this technique have increased in the
food industry because the encapsulated materials can also be
protected from moisture, heat, or other extreme conditions
(depending on the coating material), thus enhancing their
stability and extending viability and shelf life. Encapsulation
in foods is also utilized to mask odors or tastes (as in encap-
sulated fish oils). Various techniques are employed to form
the capsules, including spray drying (encapsulating in carbo-
hydrate), spray chilling (encapsulating in fat), coacervation
(typically using gelatin), and many others.
Oxygen exposure can also be limited by packaging,
modifying the storage atmosphere (modified atmosphere
packaging), and using oxygen scavengers (sachets contain-
ing powdered iron or ascorbic acid that chemically reacts
with oxygen to remove it from inside the package).
Color Changes
The preservation of color during storage depends on the
resilience of the pigment compounds present. Many of the
colored pigments are phenolic compounds that participate
as antioxidants, as they are themselves oxidized, causing
accompanying color changes in the food. General practices
that inhibit oxidative reactions and Maillard browning also
preserve pigments. Shielding the food from light (using
opaque and UV‐barrier packaging), oxygen, and metals, and
maintaining low temperatures during distribution and stor-
age, slows pigment degradation reactions.
Chlorophylls
Chlorophylls are the green pigments in green vegetables.
They also give the green color to the skin of apples and other
fruit, particularly when unripe. When green vegetables are
overheated during ordinary cooking or during canning, chlo-
rophyll is chemically transformed by a loss of magnesium
into pheophytin, causing a change of the bright green color
to brown or khaki. Exposure of green vegetables to acids can
have the same result. One way to mitigate this is to blanch
green vegetables quickly using high heat, in an excess of
water and uncovered, followed by rapid chilling. These
strategies limit exposure to heat and to acids released by
plant tissues during the cooking process. Keeping the cook-
ing water slightly alkaline by the addition of a small quantity
of sodium bicarbonate also maintains color, but the alkaline
conditions can soften the vegetable’s cell walls, leading to
mushy texture, and the bicarbonate can make the veggies
taste soapy. In addition, loss of vitamin C is increased.24
Myoglobin
The basic pigment in raw meat muscle is the heme (iron‐
containing) pigment myoglobin. (Hemoglobin is the heme
pigment in blood, which is largely removed during exsan-
guination of the animal at slaughter.) Like hemoglobin,
myoglobin contains an iron molecule that reacts with oxy-
gen, nitrite, and carbon monoxide in food processing and
during storage, resulting in different colors. When myoglobin
in fresh meat is fully oxygenated (oxymyoglobin), the muscle
is bright red and looks most appealing. When it is deprived
of oxygen (deoxymyoglobin)—for example, when meat is
packaged in oxygen‐impermeable or vacuum packaging—
the muscle turns purple. These two forms are transposable
while the meat is still fresh; if purple deoxy meat is removed
from its package and exposed to oxygen, in fairly short order
it becomes oxygenated again and turns red. Metmyoglobin,
the brownish color of aging meats, results from the oxidation
of the iron portion of myoglobin. Metmyoglobin formation
in meat in retail cases is a result of prolonged exposure to
ultraviolet light from fluorescent lights, temperature fluctua-
tions, and bacterial growth. Individual muscles accumulate
metmyoglobin, and thus discolor, at different rates. It is not
unusual for meat cuts with multiple muscle groups to dis-
color unevenly.
People typically judge freshness in meats based on appear-
ance, and as such preferentially purchase raw meats that look
bright red. Because of this, meat processors and retail
markets work to come up with packaging and merchandiz-
ing strategies that maximize fresh appearance for at least as
long as the printed expiration date on the label. Methods
include gas flushing with oxygen to maintain the bright red
color of oxygenated myoglobin (oxymyoglobin), limiting
exposure to fluorescent lighting (which favors the formation
of brown‐colored metmyoglobin), and even flushing with car-
bon monoxide (a controversial process), which binds more or
less permanently to myoglobin to give meat a persistent red
color, even when it is well past expiration and has begun to
spoil. Nitrites used in cured meats, such as ham and bacon,
also bind permanently to myoglobin, giving these meats a
permanent pink coloration.
Studies by the USDA, universities, feed manufacturers,
and vitamin manufacturers show that adding vitamin E to
cattle feed has a number of interesting results.25 Meat from
vitamin E–enhanced beef stays bright red longer (except for
top sirloin steak) and has a case life several hours longer
before color change occurs. The greatest increase is in beef
round cuts. Vitamin E also improves the stability of myoglobin
c14.indd 296 1/14/2016 7:35:00 AM
CHAPTER 14 • Shelf-Life Extension • 297
FIGURE 14.5 Pigments. Light, heat, metal ions, and oxygen accelerate oxidation reactions.
Pigment Where Found Degraded Control
Chlorophyll:
Lipid soluble,
green
Widely distributed Acid to dull olive, gray
(canned and dried green
vegetables)
Oxidation to colorless
(cabbage fades w/ storage)
Chlorophyllase enzyme
degrades to colorless
Free radicals degrade
Adjust pH
HTST processing
CA to keep cells intact
Control storage time
Heat inactivates enzyme
Antioxidants retard lipid
oxidation
Anthocyanins:
Water‐soluble
red to blue
antioxidants
Cherry, grape, radish, eggplant,
red potato, cabbage, cane
berries, currants, apples
Oxidize to colorless or brown
Enzymes (B‐glucosidase,
peroxidase, polyphenoloxidase)
degrade
Sulfites bleach @ high
concentrations (rxn reversible)
w/ metals blue‐brown
pH and oxygen sensitive
HTST preserves
inactivate
Betalains:
Water‐soluble
red antioxidants
Betaxanthins:
Yellow
Beets, Swiss chard, cactus
pear, purple pitaya (juice)
Meats, ice cream, gel
desserts
Strong acids bleach
Colorless when heat
degrades, but regenerates
pH stable 4–6
After canning, time to
regenerate color
Metal‐chelating agents
stabilize
Carotenoids:
Lipid‐soluble (most)
orange
antioxidants
Carotene (carrots, vegetables,
and fruits), lycopene,
xanthophylls (annatto),
capsanthin (peppers),
canthaxanthin (salmon, trout),
crucin (saffron); H2O soluble
Widely distributed in plants
Oxidize to colorless or brown
w/ off odor
Some heat stable, some
degrade to colorless
Oxidation fades
Lipoxygenase destroys
Annatto turns pink w/ acid
Cold-smoke preserves
Antioxidants, ascorbic
acid, and potassium
sorbate preserve
Sulfite and ascorbic acid
inhibit oxidation
Lycopenes
(a carotenoid):
Lipid‐soluble
red
antioxidant
Tomatoes, watermelon Heat can degrade cis to
less‐colored trans
Spray drying can preserve
color
Curcuminoids:
Bright yellow
fat‐soluble
antioxidants
Turmeric Oxidization fades color
Above pH 7 color shifts to
red then fades
Encapsulation preserves color
Heat, aw, radiation stable
Canned foods, mustards,
soups
Iridoids:
Water‐soluble
green and yellow
Saffron, gardenia Reacts w/ amino acids or pH:
red, blue, black
Color imitation in fish, sweets,
noodles, baked goods
Sources: Stintzing and Carle (2007);26 Ancos et al. (2000);27 Oey et al. (2008);28 Wright (1997);29 Lewicki (2005);30 Goula and Adamopoulos
(2005);31 and Delgado‐Vargas and Paredes‐Lopez (2003).32
in ground beef, providing better color retention in final
products.
Anthocyanins
The pink, red, mauve, violet, and blue colors of flowers, fruits,
and vegetables are caused by the presence of anthocyanins.
In most food processing operations, the anthocyanins are
quite stable, especially when the low pH of the fruit is main-
tained. Occasionally, however, naturally present ascorbic acid
causes problems. In the presence of iron or copper, the oxida-
tion of ascorbic acid also oxidizes anthocyanins, leading to a
loss of color.33 This can be inhibited by the use of metal chelat-
ing agents such as EDTA.
Carotenoids
Carotenoid pigments are responsible for most of the yellow
and orange colors of fruit and vegetables. Carotenoids are
divided into two principal groups: the carotenes, which are
c14.indd 297 1/14/2016 7:35:00 AM
298 • CULINOLOGY®
strictly hydrocarbons, and the xanthophylls, which contain
oxygen. Carotenoids are soluble only in fats and oils.
Although normally associated with plants, carotenoids
find their way into some animal tissues. Egg yolk owes its
color to two xanthophylls. These carotenoids, which also give
adipose tissue (tissue made up of mainly fat cells, such as the
yellow layer of fat beneath the skin), are derived from vegeta-
ble materials in the animal’s diet. Adding marigold petals to
chicken feed (as Perdue has been doing for years) increases
the yellow pigmentation in their skin.
The role of carotenoids as precursors of vitamin A is well
established.34 Carotenoids in food are stable even at high
temperatures. Fat‐soluble carotenoids are not lost to the
cooking water, but appreciable amounts may dissolve in the
butter and oils used to sauté vegetables, such as carrots.
When slices of carrot are in contact with boiling water for
two or three minutes, the hue shifts slightly toward yellow.
The longer the vegetable is cooked and the higher the tem-
perature, the greater the change in hue. But because of the
quantity of carotene present, cooked carrots remain compar-
atively bright and attractive.
Melanins
Melanin is a brownish, undesirable pigment that arises in the
tissues of fruits and vegetables that are cut or damaged. This
enzymatic browning is caused when tissue damage due to
slicing or peeling, fungal attack, or bruising brings cellular
enzymes and substrates together in the presence of oxygen,
causing melanins to form.
During fruit and vegetable processing, several strategies
are used to inhibit enzyme activity. Making sure fruits and
vegetables are blanched as soon as possible after any tissue‐
damaging operation reduces the enzyme activity to a mini-
mum. Reducing contact with air by immersion in cold,
acidified water is also a common practice. Immediately after
being peeled, fruits are often immersed in baths containing
dilute solutions of citric acid or malic acid, supplemented
with ascorbic acid or sulfite.35
Flavor Changes
Some flavor changes during storage are desirable and
actively promoted: ripening of fruits and vegetables; aging of
meats, cheeses, and wines; migration of acids and season-
ings into pickled plant or animal tissues; migration of sugars
and salts into brined fruits and meats; flavor development
during fermentation. However, chemical and physical reac-
tions can also cause flavor changes identified as spoilage
indicators. Maillard reactions in stored dairy, fruit, and veg-
etable products can degrade quality. Rancidity (hydrolytic,
oxidative), produces off flavors and off odors. Enzymes also
catalyze reactions that alter flavor compounds. Proteolysis
(the enzymatic breakdown of proteins) results in free amino
acids, and lipolysis (the enzymatic breakdown of fats) pro-
duces free fatty acids; both compounds have strong, unpleas-
ant, and bitter flavors. Flavor compounds themselves are
often susceptible to oxidation (many are phenolics), and
some are volatile. If volatile flavor compounds are bound to
FIGURE 14.6 Pigments, colors, and reactions in
raw and cooked foods.
Pigment Color Reactions
RAW
Myoglobin (Mb) Purple Natural Mb pigment
in animal muscle;
also the color of cut
meat in oxygen‐
impermeable or
vacuum packaging.
Oxymyoglobin
(O
2
Mb)
Red Mb + O
2
form
O
2
Mb; oxygenation
“blooms”; meat
turns red when
exposed to air.
Metmyoglobin
(MtMb)
Brown Mb is oxidized to
form MtMb.
Reaction favored
by low oxygen
levels, light, and
continuous storage.
Nitrosomyoglobin
(NMb)
Red‐pink Mb combined with
nitrite (cured meats:
bacon, ham).
Carboxymyoglobin Cherry
red
Mb combined with
carbon monoxide.
Sulfmyoglobin Green H
2
S produced by
microorganisms on
Mb.
COOKED
Globinhemochromogen Brown Cooked fresh meat:
O
2
Mb or MtMb is
heat denatured.
Nitrosylhemochrome Pink Cooked cured meat:
NMb denatured by
heat.
Globin N
N
N
N
Fe
Free
Binding
Site
FIGURE 14.7 Heme pigment.
c14.indd 298 1/14/2016 7:35:00 AM
CHAPTER 14 • Shelf-Life Extension • 299
proteins, lipids, or polysaccharides, they are more likely to be
retained during processing and storage; their stability
depends on pH and temperature. Flavor compounds can
also volatilize from foods into the atmosphere during heat-
ing (flashing off ).
Generally, flavors are best preserved by minimizing expo-
sure to heat, using minimal processing, using less extreme
multiple hurdles, using high‐temperature, short‐time
(HTST) processing, employing barrier storage to prevent
oxidation, including additives that preserve flavor com-
pounds and enhance flavor, and refrigerating to slow chemi-
cal reactions.
Nutritional Quality
In the best‐case scenario, foods are harvested at peak ripe-
ness and quickly processed, allowing the best opportunity for
nutrient retention. Generally, their nutrient content is similar
to fresh if optimally processed and stored properly. Nutrient
losses occur due to trimming, leaching in cleaning or blanch-
ing water, and chemical degradation (enzyme catalyzed or
thermally induced). Mechanical damage (shaking, cutting,
bruising) can unite enzymes with substrates, leading to deg-
radation. Oxidation (accelerated by oxygen, light, tempera-
ture, metal ions; slowed by blanching, antioxidant additives,
low temperature, decreased oxygen) degrades vitamins A and
C and thiamine. Loss of water-soluble nutrients (including
vitamin C, folate, thiamine, riboflavin, some sugars, proteins,
and minerals) can occur in cleaning and blanching water or
during caustic lye peeling of tomatoes and peaches with
sodium or potassium hydroxide solutions. Fermentation,
aging, and ripening are exceptions that improve nutrient
quality during storage (by eliminating anti‐nutritional com-
pounds and other digestion inhibitors; breaking down large,
indigestible materials into smaller, more easily assimilated
ones; eliminating pathogens; and enhancing palatability). But
nothing lasts forever, and overall food quality eventually dete-
riorates at a rate determined by the composition of the food,
the environment, and the packaging’s protection.36
Vitamins
The vitamins are an untidy collection of essential (required
by the body), complex, organic (carbon‐containing) nutrients
that occur in the biological materials consumed in food.
Vitamins, especially vitamin C (ascorbic acid), thiamine (B1),
and riboflavin (B2), are exceedingly unstable. The final
amount of vitamins in a food is affected by growing condi-
tions, post‐harvest changes, initial treatments (washing, mill-
ing), blanching and other thermal processes, and storage. In
addition to the effects above, the food itself and its chemical
composition influence vitamin stability. Typical reactions to
which vitamins are susceptible include:
Oxidation—fat‐soluble vitamins, vitamin C, thiamine
Degradation and other reactions (such as non‐enzymatic
browning)—vitamin C, thiamine
Photochemical reactions—riboflavin, beta‐carotene, folic
acid, fat‐soluble group
The vitamin content of fruit changes throughout growth
and ripening. Once produce is harvested, metabolic reac-
tions continue and vitamins can be destroyed. For example,
potatoes stored throughout the winter have less vitamin C
than new potatoes. Correct storage and treatment (modified
atmosphere, controlled temperature, low light, and so on)
can reduce post‐harvest vitamin loss.
After harvesting, foods undergo preliminary treatments
such as trimming, washing, and milling. Thiamine loss is par-
ticularly serious in the milling of rice. In fact, the cause of
beriberi (thiamine deficiency disease), was discovered due to
a comparison of diets featuring white rice (low in thiamine)
and brown rice (high in thiamine). The loss of vitamins dur-
ing milling led to laws requiring the vitamin enrichment of
cereal grains, flours, and bread. In the United States, proc-
essed flours must be enriched with thiamine mononitrate,
niacin, ferrous iron, riboflavin, and folic acid to replace that
lost in processing.
Thiamine (vitamin B1) is one of the most unstable B vita-
mins. Baking, pasteurizing, or boiling foods containing thia-
mine can reduce its content by up to 50 percent. The stability
of thiamine during storage depends greatly on the moisture
content of the food. Flours with 12 percent moisture content
retain 88 percent of added thiamine after five months. If the
moisture level is reduced to 6 percent, no losses occur. The
sulfite added to dried fruits and vegetables to prevent brown-
ing causes total destruction of thiamine. The commonest
losses of thiamine occur simply as a result of its water solubil-
ity rather than any chemical degradation. It is discarded with
the cooking water.
Riboflavin (vitamin B2) is stable during thermal process-
ing, storage, and food preparation. Riboflavin, however, is
susceptible to degradation when exposed to light.
Niacin (vitamin B3) is one of the most stable vitamins. The
main loss occurs from leaching into cooking water.
Pyridoxine (vitamin B6) in enriched flour is resistant to
baking temperatures. B6 is susceptible to light‐induced deg-
radation, and exposure to water can cause leaching and con-
sequent losses. Leaching is the major cause of losses during
cooking and processing. However, B6 is stable during storage.
Enriched wheat flour stored at either room temperature or
113°F (45°C) retains about 90 percent of B6.
Cobalamin (vitamin B12) is fairly stable to food processing
and cooking conditions. As with all the water‐soluble vita-
mins, leaching is the major cause of loss.37
Folic acid is unstable and loses its activity in the presence
of light, oxidizing or reducing agents, and in both acidic and
alkaline environments. However, it is relatively stable to heat
and humidity; dry mixes, baked products, and cereal flours
retain almost 100 percent of added folic acid after six months
of storage. Over 50 percent of folic acid added to wheat flour
is retained during bread baking.
Ascorbic acid (vitamin C) is quite stable in the acid, refrig-
erated environment of fruit juice processing. The stability is
c14.indd 299 1/14/2016 7:35:00 AM
300 • CULINOLOGY®
enhanced by the presence of citrate and flavonoids. Ascorbic
acid and its sodium, potassium, and calcium salts are com-
monly used as antioxidants in water‐based systems. However,
these compounds are water‐soluble and thus cannot protect
fats from oxidation. For this purpose, the fat‐soluble esters of
ascorbic acid with long‐chain fatty acids (ascorbyl palmitate
or ascorbyl stearate) can be used as food antioxidants.
Vitamin C (usually in the form of lemon or lime juice) is used
in household pretreatment dips to prevent oxidative color
changes in fruits and vegetables. It works as an antioxidant
by being preferentially oxidized instead of the food it is
protecting.
Vitamin C chemically decomposes under certain condi-
tions, many of which may occur during the cooking of food.
FIGURE 14.8 Nutrient quality table: Changes in nutrient content during shelf‐life extension strategy
and subsequent storage.
Unit Operation Nutrient Degradation Nutrient Preservation Strategy
Blanching
(heat treatment to
inactivate enzymes)
Degrades heat-labile and water-soluble vitamins
Trim, peel, wash, blanch; lose more vitamins
with pre‐treatments than during frozen storage
Slow blanch: vit C, niacin, and B
6
losses
Steam blanching is better than water
blanching.
Freezing Oxidation during storage
Thaw drip (salt, vitamins, some proteins,
amino acids, peptides)
Protein, carbohydrate, fiber, and lipid content
stable
Vit C loss high with higher-temp storage
Use antioxidants and sugar syrups, store
at low temperatures.
Rapid freeze and thaw.
Hold 0°F (–18°C) or below to retain 80% vit C.
Store below Tg (˜ –25°C) and food stable.
Canning Heat-labile vitamins
Some amino acids degraded
Water‐soluble vitamins leach
Oxidation (until O2 in headspace gone).
Vitamin C and carotenoids
Overall nutrient retention during storage
is 90–95%
HTST, nisin and acids may decrease heat
treatment.
HTST; better thiamin and B
6
retention.
Thiamin loss > w/ semisolid foods (> time/
temp). Can lose 50%.
Liquid diet importance.
Consume liquid, process w/ less.
Store cool, short times, add antioxidants.
Store cool, short times.
Temp just above freezing sig preserves.
Drying Heat-labile vitamins (60% vitamin C loss
blanching, 5% drying). Thiamin loss in
air‐dried pork is 50–70%; 5% loss in beef.
Sulfite inactivates some thiamin and B
6
.
Freeze drying w/o air preserves vit C.
Carotenoids oxidized (20–40% lost in carrots)
Protein retention good, S containing amino
acids may decrease
Sun drying; major loss of vitamins A, C,
beta‐carotene; oxidation
Lower dry temp for cereals increases thiamin
retention. Less loss w/ HTST than w/ low
temp.
Brown compounds decreased.
Carotene degrades unless pkg w/o O2.
Vitamins stable during storage w/ packaging.
Fermentation Vitamins synthesized by bacteria: niacin,
thiamin, B6, B12, ascorbic acid.
Ca, zinc, iron, Mg are preserved during bread
fermentation.
Levels increase 25–150% in various items.
Phytates (decrease metal bioavailability)
decreased during fermentations.
Pasteurization Heat degrades vit C, but if left active, enzymes
would degrade it more.
HTST best at preserving all heat‐labile
vitamins.
UHT
(130–150°C for 2–8 sec,
aseptically packaged)
Some heat-labile vitamins lost; one of the
better processes.
Cooler storage retains, though ambient
stable microbiologically
Cure and smoke Cure compounds retard oxidation Some heat-labile vitamins lost during heat
treatments, similar to cooking.
Hot smoke loses 15–20% thiamin, others
stable.
Ionizing radiation
UHP
c14.indd 300 1/14/2016 7:35:00 AM
CHAPTER 14 • Shelf-Life Extension • 301
Cooking can reduce the vitamin C content of vegetables by as
much as 60 percent. Longer cooking times and higher tem-
peratures, as in retorting, add to this effect, as do copper food
vessels, which catalyze the decomposition.
Like all water‐soluble vitamins, vitamin C is lost from food
by leaching, where the water‐soluble vitamin dissolves into
the cooking water, which is later poured away and not con-
sumed. On the other hand, fresh‐cut fruits do not lose signifi-
cant amounts when stored in the refrigerator for a few days.
Fat‐soluble vitamins (A, D, E, and K) are generally less sen-
sitive to processing effects than the water‐soluble vitamins.
Being insoluble in water, they won’t leach into wash or blanch
water, and they are more heat‐stable to cooking and thermal
processing. They are all prone to photo‐oxidation and to the
oxidizing effects of exposure to oxygen, so appropriate
opaque and air‐tight packaging yield longer shelf life.
Minerals are much more stable than vitamins in food. They
are, after all, inorganic elements—pure compounds that are
already in their simplest form. They are virtually impervious
to physical or chemical changes during normal cooking or
processing. They generally remain in the food even after cooking,
canning, or freezing, although they can leach into washing,
blanching, or cooling water, or into meat juice drippings.
Processing can affect the balance of sodium and potassium in
vegetables. Fresh vegetables are rich in potassium and naturally
low in sodium. Canned vegetables are usually higher in sodium
from added salt. All of the food groups have foods rich in miner-
als. Fruits and vegetables are good sources of potassium. Whole
grains are rich in magnesium, selenium, and chromium. Nuts
and seeds are good sources of copper and manganese. Red
meats are particularly good sources of iron and zinc.
Food Fortification and Enrichment
The way food is handled before its consumption can nega-
tively influence its micronutrient content (vitamins and min-
erals). Even with all the precautions taken to ensure the
stability of micronutrients in food, some losses still occur
during processing, distribution, and storage. One way to
counter these losses is by enrichment (adding nutrients lost
in processing) or fortification (adding nutrients not present
in the original food, such as adding proteins, amino acids,
minerals, vitamins, antioxidants, caffeine, and taurine to
sports drinks). Typically, an excess amount (an overage) of
the fortificant is added to the food to compensate for
expected processing or storage losses, to ensure that fortified
food delivers the targeted level of nutrients at the time the
food is consumed in the home.
Physical Changes
Physical changes in foods do not result in the formation of
new chemical compounds. They include the effect of gravity
(heavy particles settle), molecules going into or out of solution
(dissolving or crystallizing), separations (broken emulsions),
changes of state (evaporation, liquidification, solidifica-
tion), and molecules moving to different parts of a food
(osmosis, leaching). Some physical changes are easily reversed,
such as in redispersing heavy particles that have settled by
shaking, as in salad dressings. Others, such as textural changes
from ice crystal damage due to slow freezing, are irreversible.
Many physical changes can be inhibited by additives (emulsi-
fiers, thickeners, and so on), appropriate packaging, and con-
trolling storage time and temperature.
Change of State of Water
Liquid water becomes a solid when food is frozen, which can
cause ice crystal damage to food structure. Water can also
become a vapor and evaporate, leading to dehydration, which
is a problem in refrigerated or frozen foods (leading to
“freezer burn”) that are not well covered. Dehydration is also
a problem with long‐term storage of fresh fruits and vegeta-
bles. Moisture loss from fresh fruits and vegetables is usually
accompanied by vitamin loss and, ultimately, death of plant
cells and deterioration of quality to the point of discard. Just
the opposite, moisture uptake can lead to spoilage problems
in dried foods when water activity rises to the point where
microbial contaminants begin to grow and reproduce.38
Separations
Separations are generally physical changes, but chemical
interactions may also be involved. The most common is the
separation of water and oil in broken emulsions. In gels, such
as custards or puddings, separation (syneresis) results in a
watery layer and toughening of the solid portion as it
becomes dehydrated. Meat proteins are also in a gel‐like
structure. During freezing, more protein‐protein bonds form,
and when the meat is thawed, the displaced water, called drip
loss, leads to toughening and dryness.39
Other Factors
Influencing Shelf Life
Intrinsic Factors
Shelf life is greatly influenced by the inherent or intrinsic char-
acteristics of the food itself. The term intrinsic denotes an
innate property of the material, its genetic propensities and
predispositions. Intrinsic characteristics are independent of
how much of the material is present and the form the material
is in (for example, one large piece or a collection of smaller
pieces). Protective coverings such as shells or thick skin,
microbial resistance, and enzymatic and nutritional stability
are all intrinsic factors. So is the way different fruits ripen.
Fruits are divided by the ability to ripen after harvest
(tomatoes, bananas, avocados, peaches, pears, and so on)
and the inability to do so (citrus, grapes, melons, strawber-
ries, pineapples, and so on); the latter must achieve ripeness
before harvesting. Fruits that ripen after harvest are called
climacteric fruits, and those that do not are called non‐
climacteric fruits.
Climacteric is the final physiological process that
marks the end of fruit maturation and the beginning of fruit
c14.indd 301 1/14/2016 7:35:00 AM
302 • CULINOLOGY®
senescence (decline). Its arrival is identified by a sudden rise
in respiration of the fruit and normally takes place without
any external influences. This leads to other changes in the
fruit, including pigment changes and sugar release. For those
fruits eaten as food, the climacteric event marks the peak of
edible ripeness, with fruits having the best taste and texture
for consumption. After the climacteric period, respiration
rates (noted by carbon dioxide production) return to or below
the level before the event, and fruits are more susceptible to
fungal invasion and begin to degrade into cell death. Non‐
climacteric fruits and most vegetables do not go through this
uptick in respiration after harvest and therefore do not get any
riper than the day they were picked.
Obviously, we don’t all live close to where our produce is
grown, so if nature had its way, by the time these products got
to our supermarkets they would in many cases be past their
peak in eating quality. Luckily, shelf life can be greatly influ-
enced by storage conditions and packaging—that is, by
extrinsic factors. Balancing the interaction of intrinsic and
extrinsic characteristics is critical for prolonging shelf life.
Extrinsic Factors
Extrinsic factors are those the food product encounters as it
moves through the food chain. They include the following:
• Time‐temperature profile during processing
• Temperature control during storage and distribution
• Relative humidity (RH) during processing, storage, and
distribution
• Exposure to light (UV and IR) during processing, stor-
age, and distribution
• Surviving microbial counts during processing, storage,
and distribution
• Composition of atmosphere within packaging
• Pressure (or lack thereof ) in the package headspace
• Subsequent heat treatment (for example, reheating or
cooking before consumption)
• Transportation effects
• Consumer handling
All these factors can and do operate in an interactive and
often unpredictable way, and the possibility of deleterious
consequences must be investigated.
Stored fruits and vegetables consume oxygen and give off
carbon dioxide in a process called respiration. They continue
to live and break down the stored sugars, starches, fats, and
proteins of which they are composed. The changes caused by
respiration can be deleterious to the fruit or vegetable, lead-
ing to softening, overripening, textural collapse, and so on.
There are three ways to reduce the effects of respiration, all of
them extrinsic:
1. Reduce storage temperature.
2. Control humidity.
3. Reduce but not eliminate oxygen, or increase carbon
dioxide.
The optimum temperatures, humidity, and external gas
composition for storage vary according to the type of pro-
duce. For example, McIntosh apples are best stored at 37.4°F
(3°C), 87 percent RH (relative humidity), 3 percent oxygen
(normal = 21 percent), 3 percent carbon dioxide (normal =
0.3 percent) for about 1 month and then at 5 percent carbon
dioxide and 95 percent nitrogen.40 This type of storage is
called controlled atmosphere (CA) or modified atmosphere
(MA) storage.
Modified atmosphere packaging (MAP) of foods, both raw
and cooked, also uses this technique quite effectively. Cut fruit
and vegetables are placed in packages that are then flushed or
injected with gases before sealing to slow respiration inside
the package and extend shelf life. This has revolutionized the
produce business and allowed the creation of the enormous
bagged cut salad industry. MAP has also greatly extended the
shelf life of refrigerated packaged and prepared foods such as
deli meats, fresh pasta, sandwiches, meat salads such as
chicken and tuna, and deli salads such as potato and macaroni.
In these examples, the food ingredients have been cooked or
otherwise processed and are no longer respiring. Instead, the
MAP gases reduce or eliminate oxygen to inhibit the growth of
aerobic spoilage organisms and reduce the effects of oxidation.
This process is also commonly used in the packaging of raw
meats to retard spoilage and enhance appearance (color),
particularly in red meats. The art and science of selecting the
right mixture of gases in the most effective ratios is quite
complicated, as it must take into account whether the food
item is raw or cooked, whether its composition is uniform or
heterogeneous, what types of spoilage it is most likely to
encounter, what types of microorganisms are targeted, the
type of packaging being used, and length of shelf life desired.
Another form of MAP uses a vacuum packer to pull a vac-
uum inside the package, eliminating oxygen entirely. This is
particularly useful in foods being frozen for extended periods,
as it virtually eliminates freezer burn. Yet another version of
MAP uses high concentrations of oxygen (higher than nor-
mally found in air) and even carbon monoxide to enhance the
color of red meats.
Analyzing and
Predicting Shelf Life
The primary objective of programs and techniques for study-
ing the stability of foods is to predict their shelf life after
manufacture under normal packaging, distribution, and stor-
age conditions. It is important, therefore, for the formulator
to estimate the product’s expected shelf life. This normally
includes a period of storage by the manufacturer after
production, shipping time, possible storage in a distribu-
tion warehouse, possible exposure to elevated temperatures
(including multiple freeze‐thaw events), storage by the retailer
or foodservice operator, and the time until the product is
used by the ultimate consumer. Depending on the food in
question, shelf life can be as short as a few days (freshly made
sandwiches) to a year or more (canned or dehydrated foods).
c14.indd 302 1/14/2016 7:35:00 AM
CHAPTER 14 • Shelf-Life Extension • 303
While it is fairly easy to determine acceptable shelf life for
short shelf‐life foods, no formulator or manufacturer can
afford real‐time testing for periods up to or exceeding a year.
Instead, product developers predict shelf life on the basis of
accelerated testing programs and techniques.
To shorten the time required for predictive stability testing,
formulators subject their foods to conditions that stress the
product’s stability (“torture testing”), thereby hastening the
end of usable shelf life. It is the formulator’s responsibility to
select the stress conditions and test methodologies for which
well‐established historical precedents allow for reliable and
consistent correlation with actual shelf stability under normal
conditions. Shelf‐life prediction also takes into account esti-
mated storage and use‐up times as well as physical effects due
to loading, trucking product to the farthest destination, and
unloading. In addition, exposure to temperature extremes or
fluctuations may be called for depending on the type of prod-
uct, climate, and mode of transportation (refrigerated or unre-
frigerated trucks, railcars, freezer trucks, and so on).
When setting up stress conditions and test methodolo-
gies, formulators must employ reasonable guidelines. The
most practical approach is to rely on the comparison of a
newly created product with a similar “old standby” product
that has shown acceptable real‐life stability. Stability testing
programs are not absolute: The judgment that a product has
adequate stability is a compromise between market accept-
ance and expected instability. That said, comparison to an
established stable product is a sensible yardstick for predict-
ing the shelf life of a previously untested preparation.
Accelerated shelf‐life testing is an important part of any
product development cycle. Key findings on the chemical,
physical, sensory, and microbial changes to a challenged
product are critical in order to effectively develop, protect,
and market it. Companies may use various methods for dif-
ferent products. Developing a sound, scientific, accelerated
test takes a great deal of time and resources and should be
considered a wise investment. The goal is to have representa-
tive products under test far enough ahead of actual produc-
tion that any red‐flag problems can be identified in time to
take systematic corrective action. The manufacturer aims to
avoid being suddenly inundated with returned product and
customer complaints because the behavior of the product
during storage and distribution was not adequate.
Monitoring Effectiveness
of Food Preservation
Preservation techniques deal with how to produce foods with
extended shelf life that are also safe to eat and meet consumer
expectations of sensory quality. No matter how suitable these
techniques are, modern industrial production and processes
are not successful if they are not monitored. Many factors are
involved in ensuring safety and quality, such as the freshness
and microbial quality of raw materials and the availability of
potable water for production. Facilities and equipment must
also be continuously checked for contamination, and reme-
diations brought to bear immediately should any be found,
especially where raw meats and dairy products are processed.
In addition, a high level of worker hygiene is essential.
In addition to ongoing monitoring of microbial status,
sensory analysis of finished products over expected shelf life
is another important way to monitor the effectiveness of proc-
essing strategies. This requires panels of human assessors
who taste the company’s products daily and thus are
extremely well able to identify products that are becoming
unacceptable at an early stage. By applying statistical tech-
niques to the results, it is possible to make inferences and
gain insights about the products under test. Most large con-
sumer goods companies have departments dedicated to sen-
sory analysis, but even in smaller companies, sensory analysis
can be conducted by the QA (Quality Assurance) and
Research and Development (R&D) departments. (For addi-
tional information about sensory analysis, see Chapter 17.)
At both national and international levels, food safety legisla-
tion is extensive. Regulations are justified by the fact that food
products are reaching ever‐widening geographic distribution,
and an outbreak of foodborne illness can affect thousands and
even tens of thousands of consumers across the country or
across the globe. Because consumers cannot readily ascertain
the safety of many food products (while food spoilage is obvi-
ous, the presence of pathogens usually isn’t), it is up to produc-
ers to guarantee safety. Unfortunately, producers or retailers
are not always able to certify safety because foodborne patho-
gens are living organisms that can enter the food at many
points in the distribution chain and may propagate over time.
This inability to verify safety and the resulting potential conse-
quences for public health is the fundamental justification for
governmental intervention to improve food safety.41
A major step forward in preventing outbreaks of food-
borne illness in the United States was the creation of the
HACCP (Hazard Analysis and Critical Control Points) system
for monitoring food plant operations. HACCP was created by
the Pillsbury Company in the 1960s when it was asked to
develop absolutely safe foods for the astronauts in the U.S.
space program. Global food safety programs include GFSI
(Global Food Safety Initiative), SQF (Safe Quality Food), BRC
(British Retail Consortium), ISO 22000, and Global GAP (an
international version of HACCP). Their general purpose is to
provide a set of strict food, sanitation, and manufacturing
standards for food companies to follow that result in safe
foods consistently prepared, appropriately packaged, and
carefully tested so consumers never need worry about get-
ting ill or injured and the company need not worry about
legal woes, bad publicity, and lost business. Compliance is
monitored by third‐party auditors and paid for by the com-
pany being audited. If that isn’t incentive enough, more and
more of the largest food retailers and foodservice operators
won’t buy food products from manufacturers not certified by
one or more of the food safety organizations; they fear food‐
related lawsuits as well. So food manufacturers must become
certified by at least one of the global food safety pograms if
they wish to do business with “the big boys.” Wal‐Mart is a
prime example; in their Global Responsibility Report, they
detail how they require all of their suppliers to certify that
c14.indd 303 1/14/2016 7:35:00 AM
304 • CULINOLOGY®
they meet at least one of the GFSI internationally recognized
food safety standards.42 In the end, compliance is a win‐win
for everyone, especially consumers.
Conclusion
Once the safety of a processed food is assured, the next most
important factor to a successful food manufacture is shelf
life. The shorter the shelf life means the fresher the product.
But it also means the market for this food product is much
smaller, limited to geographical distances that allow enough
time after delivery for the product to sell before it spoils.
While there is certainly a market niche for short shelf‐life
fresh foods, U.S. consumers are accustomed to the ready
availability of most food products, everywhere and all the
time, and are not put off by many of the methods used for
shelf-life extension, nor by the idea that much of the food
they buy is more than a couple of days old.
The Culinology® professional must have a strong under-
standing of the normal minimum and potential maximum
shelf life of every type and form of raw material, food ingredi-
ent, and fully assembled finished product, as well as a solid
working knowledge of the processing methods and technolo-
gies by which salable shelf life can be extended while retain-
ing maximum eating quality and healthfulness. The success
of every new product hinges on this know‐how.
1E. B. Leib, D. Gunders, J. Ferro, A. Nielsen, G. Nosek, and J. Qu, The Dating
Game: How Confusing Food Date Labels Lead to Food Waste in America
(New York: National Resources Defense Council, 2013).
2S. J. VanGarde and M. Woodburn, Food Preservation and Safety: Principles
and Practice (New York: Wiley, 1994).
3R. P. Singh and B. A. Anderson, “The Major Types of Food Spoilage: An
Overview,” in R. Steele, ed., Understanding and Measuring the Shelf Life of Food
(Cambridge: Woodhead, 2004), 3–23.
4USDA Food Safety and Inspection Service, “Food Product Dating,” USDA
Food Safety Information (February 2007), retrieved January 16, 2001, from
http://opi.mt.gov/pdf/schoolfood/FDCP/FoodProductDating .
5J. R. Whitaker, “Principles of Enzymology for Food Sciences,” in Enzyme
Inhibitors (New York: Marcel Dekker, 1972), 255–282.
6S. Ketsa and T. Daengkanit, “Firmness and Activities of Polygalacturonase,
Pectinesterase, β‐galactosidase, and Cellulase in Ripening Durian Harvested at
Different Stages of Maturity,” Scientia Horticulturae 80, no. 3 (1999): 181–188.
7S. Badui Dergal, Química de los alimentos (México: Alhambra Mexicana,
2006).
8DANISCO. PowerFresh–Bakery Enzymes, retrieved February 21, 2011, from
http://cdn.danisco.com/fileadmin/user_upload/danisco/documents
/products/powerfresh‐bread2 .
9H. McGee, On Food and Cooking: The Science and Lore of the Kitchen (New
York: Simon and Schuster, 2007), 61–62, 143–145.
10P. A. Kreutler and D. M. Czajka‐Narins, Nutrition in Perspective, 2nd ed.
(Englewood Cliffs, NJ: Prentice Hall, 1987).
11M. Miyawaki, “Control of Polyphenol Oxidase and Pectin Methylesterase
Activities by Ultra High Pressure” (Washington State University, 2006).
12T. Robinson, B. Chandran, and P. Nigam, “Studies on the Production of
Enzymes by White-Rot Fungi for the Decolourisation of Textile Dyes,”
Enzyme and Microbial Technology 29, no. 8–9 (2001): 575–579.
13R. H. Wills, T. Lee, D. Graham, W. McGlasson, and E. Hall, Postharvest: An
Introduction to the Physiology and Handling of Fruit and Vegetables (London:
Granada, 1989).
14M. Anese and S. Sovrano, “Kinetics of Thermal Inactivation of Tomato
Lipoxygenase,” Food Chemistry 95, no. 1 (2006): 131–137.
15J. Q. Si and C. Drost-Lustenberger, “Enzymes for Bread, Pasta and Noodle
Products,” in R. J. Whitehurst and B. A. Law, eds., Enzymes in Food Technology
(Boca Raton, FL: USA CRC Press, 2002), 19–56.
16J. Entine, Let Them Eat Precaution: How Politics Is Undermining the Genetic
Revolution in Agriculture (Washington, DC: AEI Press, 2006).
17Center for Food Safety and Applied Nutrition and USDA, FDA Food Science
Research, Quantitative Assessment of Relative Risk to Public Health from
Foodborne Listeria monocytogenes Among Selected Categories of Ready‐
to‐Eat Foods (September 2003), retrieved April 11, 2011, from http://www
.fda.gov/downloads/Food/FoodScienceResearch/UCM197330 .
18Ibid.
19S. J. Forsythe and P. R. Hayes, “HACCP and Product Quality,” in Food Hygiene,
Microbiology, and HACCP (Gaithersburg, MD: Aspen, 1998), 276–324.
20Van Garde and Woodburn, Food Preservation and Safety.
21S. E. Fayle, J. A. Gerrard, and P. S. Belton, “What Is the Maillard Reaction?” in
S. E. Fayle and J. A. Gerrard, eds., The Maillard Reaction (London: Royal
Society of Chemistry, 2002), 1–8.
22W. W. Nawar, “Biochemical Aspects: Nutritional Bioavailability,” in I. A.
Taub, Food Storage Stability (Boca Raton, FL: CRC Press, 1994), 125–174.
23R. L. Whistler and J. R. Daniel, “Carbohydrates,” in O. Fennema, Food
Chemistry (New York: Marcel Dekker, 1985), 69–137.
24T. P. Coultate, Food: The Chemistry of Its Components (Cambridge: Royal
Society of Chemistry, 2002), 175–178.
25D. M. Schaefer, Fresh Beef Marketing Opportunities Due to Dietary Vitamin E
(National Cattlemen’s Beef Association and the Cattlemen’s Beef Board,
2002).
26F. C. Stintzing and R. Carle, “Betalains—Emerging Prospects for Food
Scientists,” Trends in Food Science & Technology 18, no. 10 (2007): 514–525.
27B. de Ancos, E. Ibañez, G. Reglero, and M. P. Cano. “Frozen Storage Effects
on Anthocyanins and Volatile Compounds of Raspberry Fruit,” Journal of
Agricultural and Food Chemistry 48, no. 3 (2000): 873–879.
28I. Oey, M. Lille, A. Van Loey, and M. Hendrickx, “Effect of High-Pressure
Processing on Colour, Texture and Flavour of Fruit- and Vegetable-Based
Food Products: A Review,” Trends in Food Science & Technology 19, no. 6
(2008): 320–328.
29S. Wright, S. Jeffrey, and R. Mantoura, “Evaluation of Methods and Solvents
for Pigment Extraction,” in S. Jeffrey, R. Mantoura, and S. Wright, eds.,
Phytoplankton Pigments in Oceanography (Paris: UNESCO Publishing;
1997), 261–282.
30P. P. Lewicki and G. Pawlak, “Effect of Mode of Drying on Microstructure of
Potato,” Drying Technology 23, no. 4 (2005): 847–869.
31A. M. Goula and K. G. Adamopoulos, “Stability of Lycopene During Spray
Drying of Tomato Pulp,” LWT—Food Science and Technology 38, no. 5
(2005): 479–487.
32F. Delgado-Vargas and O. Paredes-Lopez, “Anthocyanins and Betalains,” in
Natural Colorants for Food and Nutraceutical Uses (Boca Raton, FL: CRC
Press, 2003), 167–220.
33Coultate, Food, 186–190.
34Ibid., 178–185.
35Ibid., 193–194.
36S. G. Gilbert, “Stability of Nutrients During Storage of Processed Foods,” in
E. Karmas and R. S. Harris, Nutritional Evaluation of Food Processing
(Netherlands: Springer, 1988), 491–501; Coultate, Food.
37Coultate, Food, 261–300.
38Van Garde and Woodburn, Food Preservation and Safety.
39Ibid.
40N. N. Potter and J. H. Hotchkiss, “Food Deterioration and Its Control,” in
Food Science (Springer, 1995), 113–137.
41L. J. Unnevehr and H. H. Jensen, “The Economic Implications of Using HACCP
as a Food Safety Regulatory Standard,” Food Policy 24, no. 6 (1999): 625–635.
42Wal-Mart Stores, Global Responsibility Report, 2013.
c14.indd 304 1/14/2016 7:35:00 AM
CHAPTER 15 • Food Packaging • 305
15
Food Packaging
Introduction
The United States packaging supply industry has an annual
value in excess of $150 billion, of which 55 to 60 percent is for
food and beverages. Where previously packaging decisions
were the exclusive responsibility of the technical side of food
companies, as more and more manufacturers turn to multi-
disciplinary product development teams to create the next
great food product, chefs and Culinology® professionals have
also become deeply involved in package selection and testing.
Packaging is an integral part of the processing and preser-
vation of foods. It accompanies the product from beginning
to end. Its presence on the shelf influences the consumer ’ s
decision to purchase. It goes home with the consumer, remains
around the house, establishes its brand image and identity,
and contributes to brand loyalty. Selecting the proper pack-
aging system for a particular product is critical and based on
knowledge of the composition and stability of the product
and its distribution channels. A thorough consideration of
the environmental factors to which the product or the pack-
age system is exposed during distribution and storage is vital
as well.
Food and beverage packaging has multiple roles:
• To protect fresh, minimally processed, and fully processed
food and beverage products from harvest or processing
to the consumer ’ s table.
• To maintain product safety, quality, and shelf life.
• To facilitate distribution of food products from farm
to fork.
• To add convenience for consumers and foodservice
operators.
• To communicate to retailers, foodservice operators, and
consumers the product ’ s attributes, preparation instruc-
tions, nutritional value, and use‐by date.
• To comply with government regulatory requirements.
This chapter demonstrates the indispensability of food
packaging to food production, food marketing, food distri-
bution, retail grocery and foodservice operations, and the
health, safety, and nutritional well‐being of consumers.
Definitions and
Vocabulary
Food packaging is the unitization and containment of food
and beverage products to protect them against external envi-
ronmental influences such as air, microorganisms, dirt, light,
moisture, impact, vibration, and insects—from harvest, man-
ufacture, or preparation to consumption.
For the food processor, food packaging is arguably the
most effective means of delivery to and communication with
the consumer. Without food packaging, no industrialized
country ’ s economic system could function. Instead, most of
the population would be immersed in growing and preserving
its own food. Coupled with the distribution infrastructure and
modern technology, food processing and packaging are the
most cost‐, energy‐, and resource‐efficient means of delivering
safe, uniform‐quality food for mass consumption. In fact, only
about 7 percent of the retail price for food and beverages
comes from the packaging.
The products that food scientists, technologists, Culinology®
professionals, and chefs develop, and manufacturers produce,
cannot be delivered to consumers without some form of protec-
tive packaging. Nearly every food product is packaged in some
way at some point in its trip through the distribution system.
What Is Packaging?
A package is a physical structure for containment and
protection.
“ Packaging ” encompasses the sum of associated tech-
nology, equipment, and materials, and their interactions with
Lead Authors: Dr. Aaron L. Brody , Adjunct Professor, University of Georgia Department of
Food Science and Technology , President/CEO, Packaging/Brody
Mark Thomas , President , MDT, Ltd .
Contributor: Thomas Trimarco Jr. , Operations/Production Manager , Greencore Rhode Island
c15.indd 305 1/22/2016 1:16:48 PM
306 • CULINOLOGY®
the contained food products. Packaging plays an indispensable
role in the food and beverage processing and distribution busi-
nesses, including all forms of wholesaling, retailing, grocery,
and foodservice.
Packaging Definitions
• Primary packaging—The glass and plastic bottles and
jars; plastic cups, trays, and tubs; metal and composite
paperboard canisters; flexible pouches; bags; and many
others that are in direct contact with the food. Packaging
is the first and main line of protection against moisture
and oxygen loss or gain, microbiological contamination,
insects, tampering, and so on.
• Secondary packaging—This unitizes multiple primary
packages—for example, bottles of beer packaged together
in a six‐pack paperboard folding carton or banded together
with a plastic six‐pack ring, to facilitate retail sale.
• Distribution, or tertiary packaging—An external package
that facilitates the movement of large quantities of
packaged foods—for example, a paperboard folding car-
ton or a corrugated fiberboard shipping case or plastic
film–wrapped corrugated fiberboard tray or wooden
pallet that permits the assembly of several primary or
secondary packages, such as multiple 6‐ or 12‐packs for
retail sale or 24‐packs for shipment from the packager to
retailer.
• Packaging technology—The application of scientific
principles to protect contained products against
microorganisms, moisture, oxygen, light, shock, vibra-
tion, impact, compression, human abuse, tampering,
etc., while engineering materials to minimize insult to
the environment. The means to predict and measure
shelf life of the contained food or beverage product is
also involved in determining package effectiveness.
• Structure—The physical entity of a package, such as a
carton, can, bottle, jar, tub, cup, tray, case, or pouch.
• Converting—Transforming packaging raw materials—
paper, glass, plastic, metal—into finished package
structures.
• Graphics—The surface appearance and decoration of
a package—its shape, form, and color—including the
brand name or the trade dress and trademark. Package
graphics provide recognition, identification, and com-
munication to the outside world of what is inside the
package.
The Functions of Packaging
Packaging should provide performance at an affordable cost,
usually much less than 10 percent of the total retail price of
the product. It extends the food supply in industrial countries
by significantly reducing food and beverage waste to below
30 percent of the input supply.
Packaging also communicates the identity of the package’s
contents, including product regulatory label requirements,
positioning for retailers, and information and preparation
instructions for consumers.
Information for consumers can include instructions for
end use—preparation instructions (with variations as needed);
best‐if‐used‐by date; dispensing and reclosure; disposal or
recycling of spent package materials; promotional and mar-
keting information; and nutritional information.
Although the basic communications function of food pack-
aging are identification, compliance, and useful information,
passive and active linkages between consumer and marketer
have become overt relationships. The package is no longer an
inert or benign connection; it is an active two‐way transmis-
sion engineered to inform, respond, and influence both par-
ticipants. Package graphics and structures are now designed
to attract, alert, and persuade, all in a single swift action.
Color, shape, reflectivity, disruption, and provocation are
integrated with the total marketing experience, including
sound, sight, aroma, and suggestion to the consumer and user.
Packaging is an excitement generator—an essential exten-
sion of the eating experience—with more to come as we learn
better how to use it to complement food.
Finally, packaging protects the contents from the natural
world of air, earth, water, light, and animals, including
birds, insects, rodents, and microorganisms that attack
food. Successful packaging also takes the environment into
account and is designed to avoid excess and unrecyclable
waste materials.
A Brief History
of Packaging
The history of humankind is written in its packaging—leaves,
urns, pottery, amphorae, woven reed baskets, leather pouches,
and so on. Packaging of food and water gave humans the abil-
ity to hunt and gather far from the home site, to explore and
travel, and to colonize new lands and build cities. In ancient
times, people employed primitive packaging such as leaves,
animal skins, and even skulls to contain foods. Pottery and
glass were used as far back as 5000 years ago. The origins of
modern food packaging lie in the nineteenth century with
the beginnings of canning (explored by Nicholas Appert,
Louis Pasteur, Samuel C. Prescott, William L. Underwood,
and Charles Olin Ball), papermaking, and package material
converting. Glass bottle‐making on an industrial basis began
at the turn of the twentieth century.
During the early 1900s, commercial food and beverage
canning was developed. Food freezing was engineered during
the 1920s; cellophane and aluminum foil were invented dur-
ing the pre–World War II period. The first practical plastics,
such as polyethylene, polypropylene, and nylon, were invented
prior to the 1950s, and today plastic packaging comprises
well over 60 billion plastic bottles and jars plus film, clam-
shells, pouches, bags, and many other structures.
Many current package materials, concepts, and machinery
were developed after the 1950s, including much of the pack-
aging in which we process and distribute food and beverages
c15.indd 306 1/14/2016 7:35:49 AM
CHAPTER 15 • Food Packaging • 307
today: aluminum cans for carbonated beverages, beer, and
juices; aseptic shelf‐stable juice paperboard carton packaging
for beverages, milk, and soup; and vacuum packaging for all
manner of meats and poultry, both raw and cooked.
Flexible—that is, plastic—packaging now represents
about 20 percent of the total in value, and its use is growing
rapidly, but it is actually much higher in volume when you
consider the surface area it covers. This includes laminates
(multi‐material layers joined together by adhesives or heated
layers; the layers may consist of plastic and non‐plastic
materials—for example, paper and foil), coatings, and coex-
trusions (the layers are joined by heating to the point of
melting; all layers must be plastic). A comprehensive example
is a three‐layer coextruded film where the inner film layer
provides barrier properties, the middle layer provides the
necessary strength or stiffness to the final construction, and
the third layer, on the outside, provides a printable surface.
The following packaging technologies have evolved in just
the past 40 years:
• The polyester carbonated‐beverage bottle, now also used
for salad dressings, water, sauces, juices, and toppings,
introduced in the late 1970s
• Microwavable packaging, beginning in the 1980s and
now often a key to prepared foods distribution
• Extended refrigerated shelf‐life (ESL) packaging for bev-
erages and chilled prepared foods
• Oxygen‐barrier plastics, such as ethylene vinyl alcohol
(EVOH)
• Multilayer plastic barrier packaging for retort bottles and
trays, replacing more expensive and heavier metal cans
• Aluminum bottles, as contrasted to cans, widely com-
mercialized for beverages in 2002
• Retortable paperboard composite cartons, developed
in 2003 and 2004 and still seeking a niche in North
America
• Active packaging (oxygen‐scavenging, antimicrobial,
moisture‐absorbing) and intelligent packaging (provid-
ing time and temperature history, microbial growth
indicators, and so on)—recent phenomena that have
still not achieved full commercial success. However, oxy-
gen‐scavenger polyester bottles (with scavengers in the
plastic) and cereal boxes with antioxidants impregnated
in the film liners are currently in use.
Today, food and beverage packaging in the United States
represents more than 600 billion individual units annually.
• Metal is represented by about 130 billion cans, including
105 billion two‐piece aluminum beverage cans (for beer,
carbonated beverages, and juices).
FIGURE 15.1 Vacuum‐packaged beef strip loin,
introduced during the 1960s.
Erich Eggimann/Getty Images, Inc.
FIGURE 15.3 Typical flexible pouch for salty
snacks.
Lee Rogers/Getty Images, Inc.
FIGURE 15.2 Polyester carbonated‐beverage
bottle, introduced in 1977.
Steve Snyder/Getty Images, Inc.
c15.indd 307 1/14/2016 7:36:08 AM
308 • CULINOLOGY®
• Glass is declining to fewer than 40 billion bottles and jars
annually.
• Wood is still used in pallets, crates, and some specialty
primary packages.
• Plastic has grown to well over 60 billion packages, but
actual volumes are much higher when wraps and films
are taken into account.
The Packaging
Supply Industry
Food and beverage processors, packagers, distributors,
and retailers depend on their suppliers for packaging,
technology, and research needs. The following organiza-
tions offer a wealth of information to help the product
developer select the best materials for containment of
each food or beverage.
• Raw material suppliers—plastic resin (polyethylene, poly-
propylene, polyester) makers (such as DuPont, Dow,
ExxonMobil); metal suppliers (such as Alcoa and Alcan);
paper and paperboard makers (such as International
Paper, MWV, RockTenn), and many others.
• Converters—organizations that transform raw materials
into useful package materials—oriented polypropylene
film, paperboard folding cartons, corrugated fiberboard
cases, polyester bottles; metal cans (such as RockTenn;
Printpack; Cryovac [a division of Sealed Air]; Consolidated
Bottle).
• Equipment manufacturers—makers of converting equip-
ment such as printing presses, winders, die cutters,
slitters, extruders, and molding presses used to fabricate
package materials; also makers of equipment such as
canning, pouching, cartoning, wrapping, and casing
machines used directly by food and beverage packagers
to link food and beverage contents to the package, for
example, Kliklock-Woodman, Rovema, Bosch.
• Component suppliers—manufacturers of small items
such as labels, glue, coding, closures, tags, and items like
can seam measuring instruments, all essential to the
completion of the packaging but not major in terms of
cost. An example is Avery Dennison.
• Designers—developers of the structural designs of paper-
board folding cartons, bottles, and corrugated fiber-
board shipping cases; also graphic designers.
• Packaging research firms—providers of objective insights
into consumer and retailer reactions to the graphics and
package forms. Examples are universities such as
Clemson and Michigan State; National Food Labs.
• Package testing firms—physical testers of packaging to
determine protective characteristics, such as water
vapor transmission and impact resistance, and testers of
shelf‐life characteristics. Examples are universities such
as Clemson and Michigan State; National Food Labs.
• Consultants—providers of marketing data, information,
insights, strategies, systems integration, machinery link-
ages, services, and so on. Examples include Packaging/
Brody, Inc., MDT Ltd., HAVI, and Packaging Technology
and Research.
• Conferences and exhibitions—mass information deliver-
ers of packaging information (such as Interpack in
Dusseldorf, Germany, and Tokyo Pack in Tokyo, Japan).
• Publishers—developers and suppliers of books and jour-
nals. Examples include CRC, IFT, DEStech, and Wiley.
• Professional and trade associations—information sources,
such as:
• Package Machinery Manufacturers Institute (PMMI)
• Institute of Food Technologists (IFT), Food Packaging
Division
• Paperboard Packaging Council (PPC)
• Flexible Packaging Association (FPA)
• Institute of Packaging Professionals (IoPP)
• Research Chefs Association (RCA)
• Universities—educators of packaging technologists and
researchers in the field; institutions located in the United
States and Canada include:
• Michigan State University
• Clemson University
• Rochester Institute of Technology
• Rutgers, the State University of New Jersey
• San Jose State University, California
• Indiana State University
• California Polytechnic Institute
• University of Wisconsin, Stout
• University of Guelph, Ontario, Canada
• Universities with food science and technology
departments
• U.S. government agencies relevant to food packaging:
• Food and Drug Administration (FDA)
• Department of Agriculture (USDA)
• Department of Transportation (DOT)
• Department of Commerce
• Treasury Department
• Basic packaging research—in Europe:
• United Kingdom: Campden and Chorleywood Food
Research Association (CCFRA)
• Netherlands: TNO, Netherlands Organisation for
Ap plied Scientific Research
• Sweden: SIK Swedish Institute of Food Preservation
Product Distribution
Packaging plays a vital role in distribution of the finished
product from the end of the food or beverage production line
to the retailer or foodservice operator and ultimately to the
consumer. It involves logistics, warehousing, transportation,
retailer requirements, display, economics, traffic and ship-
ping, and promotional packaging.
c15.indd 308 1/14/2016 7:36:08 AM
CHAPTER 15 • Food Packaging • 309
Packaging in the grocery industry has come a long way
from the days of backroom molded-pulp meat trays and cel-
lophane film wrapping. While these types of packaging are
still popular, the grocery industry realizes consumers have
many more food options than ever before, and manufacturers
need to find a way to draw buyers to their products. Grocery
chains are competing in a new market, introducing conveni-
ent, fully cooked prepared meals‐to‐go and trying to take
sales away from Quick Service Restaurants (QSRs) and other
foodservice take‐out offerings. Packaging plays a strong role
here because consumers expect the meal taken home from
the grocery store to have the same quality and look it would
have in a restaurant. On the other side, the foodservice mar-
ket is focusing its efforts on a larger selection of higher‐qual-
ity products while remaining price‐sensitive. To help in both
these areas, the packaging industry is continually pushing
the envelope with new designs and new materials.
Food and Beverage
Distribution
Manufacturers such as Susanna’s Kitchens, wholesalers such as
Sysco, and retailers such as Kroger constitute most of America’s
food channels that enable the delivery of safe, high‐quality food
to consumers. During the past 25 years, the boundaries
between foodservice and retail have blurred; many groceries
have installed sit‐down dining areas, and many restaurants
offer packaged take‐out foods prepared in their own kitchens.
About half the dollar value of food and 40 percent of
its volume passes through foodservice channels; the rest goes
through retail grocery channels. All, of course, initially passes
through industrial channels—that is, food manufacturers.
Foodservice Distribution
Channels
The 653,000 foodservice outlets in the United States
can be classified into three broad categories—commercial,
FIGURE 15.4 Pallet loads of cases for
transportation.
gilas/Getty Images, Inc.
contractor‐managed, and non‐commercial—all of which
require different forms of food packaging.
Commercial restaurants are those whose primary busi-
ness is preparing and serving food to customers for profit.
Examples of commercial restaurants are full‐service restau-
rants, QSRs, caterers, and cafeterias.
Contractor‐managed foodservice operations, whose prin-
cipal business is preparing and serving food to internal con-
sumers under long‐term contracts, are found in businesses,
hospitals, colleges, sports centers, military posts, and airlines.
In non‐commercial foodservice, the primary business is
something other than foodservice, and food is provided as an
adjunct. Establishments in this category operate their own
foodservice facility, as opposed to having a contractor man-
aging the operation. Examples include employee cafeterias,
transportation (airlines, trains), school lunch programs, and
nursing homes.
Retail Grocery Distribution
Retail food distribution has changed radically during the past
30 years due to the influence of mammoth box and discount
stores. The United States has about 30,000 supermarkets,
3000 box stores, and 110,000 convenience stores, plus numer-
ous kiosks, specialty and ethnic shops, catalog and online
ordering, vending machines, and assorted other outlets.
About 30 percent of packaged food and beverage distribution
is direct delivery to the retail outlet through individual sales
(carbonated beverages, beer, bread, salty snacks), with the
remainder going through distributor warehouses. Inventories
tend to be higher and shelf life must be longer for warehoused
products.
In retail channels, about 15 percent of the food and bever-
age movement is home meal replacement, about 40 percent
is chilled, and 5 or 6 percent is frozen. The majority of foods
in retail distribution are ambient-temperature shelf-stable
packaged goods such as canned and dehydrated products.
FIGURE 15.5 The interior of a supermarket is
a cornucopia of fresh and processed food—
regardless of location.
Burak Demir/Getty Images, Inc.
c15.indd 309 1/14/2016 7:36:34 AM
310 • CULINOLOGY®
Food Science,
Food Technology,
and Culinology®
Research chefs involved in new product development often
pay little attention to packaging, using materials already on
hand without much thought about their importance. The
assumption is that packaging needs for new products are the
same as for current products. But is that assumption correct?
What individual product characteristics drive, or define,
the appropriate package?
• Required shelf life—The longer the shelf life, the better
the oxygen and moisture, and in some cases, light bar-
rier that is required.
• Storage temperature—Ambient, refrigerated, or frozen?
Ambient-temperature shelf‐stable foods require a better
barrier against moisture and oxygen transmission; frozen
foods require more moisture protection; chilled foods
need multiple barriers.
• Physical protection requirements—The longer the dis-
tribution channel, the greater the protection require-
ments against vibration, impact, compression, and
contamination.
• Security—Tamper indicators are essential.
• Light permeability—Opaque packaging helps protect
against photo‐oxidation.
• Contents visibility—Light sensitivity must be balanced
against product visibility for marketing purposes.
• Vapor barrier—Almost all foods must be protected
against moisture transfer in or out.
• Acid resistance—Microbiological safety can depend on
low pH (below 4.6); in these cases, packaging must be
able to withstand, maintain, and even promote an acidic
environment.
• Oxidation and rancidity prevention—Oxygen is almost
always adverse to product shelf life; it must be controlled
or even eliminated.
• Hot fill or retort capability—Many plastics cannot with-
stand hot fill or retort temperatures and pressures.
• Minimal use of packaging materials—The less the pack-
age material mass, the lower its cost and environmental
impact. Many retailers are now demanding that manu-
facturers provide their products in simpler, more effi-
cient, “frustration‐free” packaging.
• Ecologically smart—Desirable packages are recyclable,
reusable, or easily broken down to low‐impact, nontoxic
materials.
• Marketing image—The package is designed to attract
consumer trial and repeat purchase at retail.
• Consumer information—The package is designed to
communicate with target consumers.
• Regulatory requirements and nutritional data—The pack-
age carries information required by law or regulation.
The preservation and protection of virtually every type of
food is enhanced by packaging: meats, fish, produce, beverages,
bakery goods, dairy products, prepared dishes—the list is
endless. For each there must be a means to delay or prevent
potential spoilage and quality loss. Education, experience, and
training in food packaging are essential for identifying and
applying the best systems for optimizing food safety and qual-
ity retention. Food packaging technologists depend on the
experience and knowledge of research chefs, and vice versa.
Cooperation among these professionals is essential; too much
is involved for one person to know all of the relevant variables.
Microbiological Safety
Microbiological food safety combines processing, sealing
against recontamination of the product, and protection
through distribution channels. Recontamination control
through hermetic closure of food packaging is a must.
Nearly half of all the food and beverage products in the
United States are fresh or minimally processed and typically
distributed at refrigerated temperatures to retard spoilage
and quality loss. Fresh food products include meats, poultry,
seafood, vegetables, and fruits that are unprocessed except
for removal from the original environment and limited trim-
ming and cleaning. They are handled under sanitary condi-
tions to retard microbiological and enzymatic deterioration,
which is relatively rapid at ambient or higher temperatures.
Most fresh vegetables and fruits are chilled to below 50°F
(10°C) for distribution; meats, seafood, and poultry are chilled
to 40°F (4°C) and may be frozen to enhance preservation.
It is important to recognize that many variables other
than package structure must be in place for packaging
functionality to be fulfilled. For example, when high‐ or low‐
oxygen packaging environments are applied to retain color
of fresh meats, gas barrier films are required to hold in the
gas. On the other hand, in bagged fresh produce, where res-
piratory anaerobiosis leading to rapid deterioration is to be
avoided, semipermeable films are required to allow for res-
piration (albeit slowed by refrigeration) to continue.
Minimally processed foods such as cottage cheese and
yogurt have been altered by fermentation or direct acidifica-
tion to reduce pH, thereby minimizing deteriorative processes
and extending shelf life. These are usually refrigerated at 40°F
(4°C) or lower (but above freezing). Shelf life is usually meas-
ured in weeks and even months when sealed plastic tray or cup
packaging is used to retard microbiological contamination
and moisture transfer. The newer extended shelf life (ESL)
food products are often packaged under reduced‐oxygen
conditions in barrier plastic film pouches and bottles. Fully
processed foods are intended for long‐term shelf life—that is,
months—at ambient or freezing temperatures and include
most heat‐processed, canned, dried, and frozen foods.
Fresh Foods
About 25 percent of food products in the United States are
meats, including beef, poultry, pork, seafood, lamb, and veal.
All are susceptible to microbiological, enzymatic, biochemical,
c15.indd 310 1/14/2016 7:36:34 AM
CHAPTER 15 • Food Packaging • 311
and physical changes. Fresh meat safety is a key issue because
meat is subject to growth of pathogens, and so distribution
conditions must be carefully controlled. These foods also
tend to be the costliest.
Several types of packaging work well for muscle meats,
poultry, and fish. Polyvinyl chloride (PVC) is a common
stretch or cling film used for overwrapping fresh meat, poul-
try, and produce trays in retail stores to provide a transpar-
ent, low‐moisture‐permeable, high‐gas‐permeable material
that can retard respiratory anaerobiosis in produce and
provide adequate oxygen to retain the red color of fresh
meats. While the traditional expanded polystyrene meat
tray and PVC wrap works well, it does not help extend the
life of the product. Many companies are turning to modified
atmosphere packaging (MAP).
MAP is a technology for prolonging the shelf life of fresh or
minimally processed foods. The gaseous environment sur-
rounding the food inside the package is modified: the normal
ratio of gases in air (21 percent oxygen and 0.038 percent car-
bon dioxide) is adjusted to reduce the oxygen level to 10 to 15
percent and elevate the carbon dioxide to 5 to 20 percent
inside the package. This slows the natural oxidative deterio-
ration of the food under chilled conditions. The specific blend
of gases varies depending on the product, the packaging
materials, and storage temperature.
Unlike most other commodities, which survive longer
when packed under low or no oxygen, the color of fresh red
meat depends on the presence of high levels of oxygen to
maintain the fresh red color. The normal color of the meat
pigment myoglobin is purple. When exposed to oxygen, myo-
globin turns into oxymyoglobin and the color changes from
purple to bright red. In distribution channels, most red meat
is packaged under reduced oxygen in oxygen and water‐vapor
barrier packaging to retard microbiological deterioration. As
an additional hurdle, fresh meats are transported to retail
outlets at temperatures at or below 40°F (4°C) to retard dete-
riorative processes. By the time it reaches the retailer, the
meat color can be quite dark due to lack of oxygen. For mer-
chandizing purposes in the grocery store, the red color of
meat can be revived through the use of appropriate MAP (in
this case, with higher oxygen in the package) combined with
gas-barrier packaging.
Raw poultry is an excellent substrate for infectious
salmonella and Campylobacter microorganisms. In fact, most
fresh poultry sold at retail is already contaminated with these
bacteria by the time it reaches supermarket shelves. This
occurs even though carcass temperatures are reduced as
rapidly as possible after slaughter, often by ice or cold‐water
immersion or air cooling and shipping on ice. Most poultry is
prepackaged in expanded polystyrene trays wrapped with
moisture‐barrier plastic film to retard moisture loss and
microbiological growth under reduced temperature. Once
again, sanitary processing and temperature control are the
first essentials to preservation, complemented by sound pack-
aging. The consumer must then make sure that raw chicken is
handled carefully to prevent cross‐contamination of other
foods and that it is always fully cooked before consumption.
Seafood is chilled and packaged in water‐vapor barrier
packaging, such as PVC film wrap on expanded polystyrene
trays, to retard microbiological and enzymatic activity and
weight (moisture) loss, then held at refrigerated temperatures.
Packaging for frozen fish generally has low water‐vapor per-
meability to permit long‐term frozen distribution without
freezer burn or surface desiccation due to sublimation. Some
MAP of seafood in barrier plastic trays with hermetically
sealed barrier lidding has been commercialized, but again,
temperature control is the key.
Many soft‐bodied fresh fruits and vegetables must be
handled gently because of sensitive physical structures, poten-
tial for increased enzymatic activity in damaged flesh, and the
ubiquitous presence of surface microorganisms that can pen-
etrate damaged or bruised areas in the flesh or enter through
the stem area. Damage to the product’s surface provides chan-
nels through which microorganisms can enter to initiate spoil-
age. Temperature control is quite specific, as many fresh fruits
especially can be damaged by over‐chilling. Most leafy vegeta-
bles can be extended in shelf life by packaging under specific
modified atmosphere (MAP) conditions (oxygen reduced from
21 to 2 percent, carbon dioxide increased from 0.4 to 5 percent)
and with gas‐semipermeable plastic film packaging to retard
anaerobic respiration that results from a near‐zero oxygen
condition as would occur in an airtight bag. As long as
refrigerated temperature storage is maintained, this can
increase bagged salad shelf life to as long as 15 days, whereas
under air it would remain fresh for less than 4 or 5 days.
Partially Processed Food Products
Partially processed food products receive more than minimal
processing—for example, cooking, curing, or smoking—but
still require refrigeration.
Cured Meats
Ham, bacon, sausage, bologna, and so on are cured to reduce
water activity and thus retard microbiological and biochemi-
cal deterioration, are seasoned for flavor, and usually contain
other ingredients (typically nitrites) to maintain the desired
pink color and inhibit the growth of pathogens. Curing agents
include salt, sodium nitrite, ascorbic acid, sugar, and sodium
nitrate. Often such products are smoked, or cooked and
smoked, for flavor and to enhance preservation. Cured meats
are usually stored in the absence of oxygen to achieve refrig-
erated shelf lives measured in weeks. Packaging for such
products are usually gas‐barrier plastic films to provide either
vacuum or inert gas–flushed (for example, nitrogen) internal
environments that retard microbiological growth and en –
zymatic or biochemical change.
Vacuum packing is a form of MAP because it involves
removal of the atmosphere inside the package. It is highly
effective in extending the shelf life of many non‐respiring
foods. That said, vacuum packing by itself does not make
foods sterile; high‐moisture foods still must be refrigerated or
frozen. Vacuum packing is an effective way to inhibit the
growth of aerobic spoilage organisms, retard the deleterious
c15.indd 311 1/14/2016 7:36:34 AM
312 • CULINOLOGY®
effects of oxidation, and maximize retention of sensory quali-
ties of the food (taste, aroma, texture, moisture). It also helps
prevent dehydration (freezer burn) during long‐term frozen
storage.
Dairy Products
Dairy products must be heat‐treated to eliminate pathogens,
denature enzymes, and reduce overall microbial counts.
Pasteurization is a relatively mild heat process that destroys
pathogens and deteriorative enzymes but not all microorgan-
isms. Pasteurized dairy products must therefore be refrigerated
after processing to retard microbiological spoilage. Ultra‐high‐
temperature (UHT) processing offers extended shelf life, but
only under refrigeration, as the package may not be sterile. In
aseptic packaging, milk (or other fluid food) is heat‐sterilized—
that is, rendered free of spoilage and pathogenic microorgan-
isms and enzymes. Simultaneously, high‐barrier paperboard,
aluminum foil, plastic lamination, or all‐plastic barrier packag-
ing material is also sterilized. The milk and packaging are
brought together in a sterile environment where the package is
assembled, filled, and hermetically sealed to produce sterile
milk in a sterile package that can be distributed under ambient
(room temperature) conditions. Aseptic technologies are also
applied to products for Extended Shelf Life Re frigerated
(ESLR), but the package structure may not be sterile and so the
product must be refrigerated.
FIGURE 15.6 Aseptic processing and packaging
have enabled food processors to offer a wide range
of safe, high‐quality liquids and beverages.
N_design/Getty Images, Inc.
Fully Processed Foods
Canning is a way to process foods so they are shelf‐stable at
room temperature. Once they are canned, even perishable
foods can be stored without refrigeration. Most fully proc-
essed foods are processed and packaged so that ambient
room temperature shelf life can exceed 6 to 12 months.
The canning process thermally destroys all microorganisms
and enzymes and maintains commercial sterility by hermeti-
cally sealing foods in oxygen‐ and water vapor–impermeable
packaging. Whether a metal can, glass jar, or plastic pouch or
tray is used for packaging, the process begins with treating
the raw food product before filling. Entrapped air that can
cause oxidative damage is largely removed from the food
contents by heating (cooking or blanching) before filling.
Once the packages are filled and hermetically sealed, anaero-
bic conditions inside the package can foster the growth of
anaerobic spore‐forming pathogens such as Clostridium botu-
linum if such spores are present, if pH is 4.6 or above, and if
the proper temperature conditions for germination exist.
Therefore, the food must be heated after packaging to 212°F
(100°C) for high‐acid products (pH lower than 4.6), and to
250°F (121°C) for low‐acid products (pH higher than 4.6) to
ensure the destruction of pathogenic spores.
In order to achieve temperatures above 212°F (100°C; nor-
mal boiling point of water at sea level), water must be placed
under pressure. Therefore, the packages must be able to with-
stand the pressure (typically 15 psi). The canning process is
calculated on the basis of time required for the slowest heat-
ing location of the food within the package (known as the
cold spot, usually the center of the package) to achieve a tem-
perature that destroys Clostridia spores within a reasonable
period. After reaching that temperature (typically 250°F
[121°C]), the package must be held for a prescribed amount
of time that depends on the type of food, and then cooled
rapidly to stop further cooking, which degrades the product.
FIGURE 15.7 Classic steel cans that have been
sterilized by post‐fill high‐pressure retort
processing for ambient‐temperature shelf‐stable
distribution.
NoDerog/Getty Images, Inc.
c15.indd 312 1/14/2016 7:36:47 AM
CHAPTER 15 • Food Packaging • 313
Freezing
Freezing reduces the temperature of the food to below the
freezing point of water so microbiological, enzymatic, and
biochemical activities are virtually halted. In commercial
freezing, the product temperature passes through the transi-
tion from liquid water to ice rapidly so the ice crystals that
form are relatively small and do not physically disrupt the
food’s cell structure. Depending on the product, freezing may
take place either before or after packing. Most freezing
processes use high‐velocity cold air or, less frequently, liquid
nitrogen or carbon dioxide snow to remove heat from solid,
bulk, or individually quick frozen (IQF) products.
Frozen food packaging must prevent the loss of water
vapor, as the low relative humidity and constant migration of
moisture within the freezer to the freezer coils readily dehy-
drates improperly packaged foods. The dehydrated areas lack
desirable color, f lavor, and texture. Such defects, termed
freezer burn, occur in frozen foods because of extended stor-
age in non‐airtight or damaged packaging. Proper packaging
must also allow for water content expansion during freezing;
thus, the material should be strong yet flexible. The fill volume
must be controlled accurately to avoid overfilling or under-
filling. Unfilled spaces may permit ice crystal migration from
the food, which can cause freezer burn, while overfilled pack-
ages may rupture when frozen due to product expansion.
Packages that protect their contents from light and odors
also extend their shelf life—that is, they retard oxidation of
lipids and pigments. Vacuum packaging reduces oxygen and
void volume, which increases product stability and eliminates
internal ice crystal formation (cavity ice). Further, vacuum
packaging reduces overall product volume by compression,
which benefits shipping and handling.
Throughout the history of frozen foods, the package has
often served as the preparation, heating, and eating vessel
(think TV dinners), and since the microwave oven has
become the preferred rethermalizing unit, increasingly
sophisticated packaging structures have been developed to
take advantage of this.
Dehydration
The removal of moisture retards food deterioration by
eliminating almost all water required for the growth of micro-
organisms and for most biochemical and enzymatic reac-
tions. Liquids (such as coffee or juice), semisolid foods (such
as mashed potatoes and oatmeal), and solid foods (such as
fruit, like raisins, dates, and figs, and meat, like jerky) can
all be dried by an assortment of techniques, including spray‐,
drum‐, vacuum‐, and air‐drying as well as lyophilization
(freeze‐drying). The shelf life of dehydrated foods is main-
tained by appropriate protective packaging. Suitable flexible
films with low water vapor transition rate and low oxygen
permeability are optimum for long‐term shelf life.
Deterioration of dehydrated foods is highly dependent on
the type of food and its water activity (a
w
) after processing.
Many dehydrated food products have a moisture content of
less than 10 percent (approximately a
w
0.5) and are extremely
FIGURE 15.8 Breakfast cereal product is pack-
aged in coextruded plastic film that provides both
moisture and fat barrier, with an outer paperboard
carton offering physical protection to fragile
contents and a large communications billboard.
DebbiSmirnoff/Getty Images, Inc.
stable. However, spoilage may occur by oxidation and the
onset of rancidity and discoloration (as when a highly porous
surface area enables rapid penetration of oxygen, which may
be mitigated by nitrogen flushing). Moisture uptake can cause
loss of stability, potential for microbial outgrowth, clumping,
and caking. Over time there can be flavor changes (loss of
volatiles or entry of off flavors), mechanical damage, or infesta-
tion by insects. Further, exposure to visible and/or ultraviolet
light may dramatically accelerate quality degradation.1
Fats and Oils
Cooking oils and hydrogenated vegetable shortenings con-
tain no water and so, in theory, are microbiologically stable at
ambient temperatures. Unsaturated fats and oils, however,
are vulnerable to oxidative rancidity; therefore, both are usu-
ally packaged under a low‐oxygen atmosphere, such as nitro-
gen or vacuum, and often contain antioxidants and chelating
agents (tocopherols, EDTA) to retard rancidity. Packaging for
oils and fats obviously must be lipid‐resistant and also have
fairly good oxygen barriers such as polyester bottles or foil‐
lined paperboard cans (for shortenings such as Crisco).
Margarine and butter contain fat plus water and water‐
soluble ingredients—that is, salt and milk solids that impart
flavor and color to the product. Generally, these products are
distributed at refrigerated temperatures to retard microbio-
logical growth and rancidity. Butter sticks are commonly
wrapped in parchment paper, which helps maintain the
quality of the butter; the paper is fat‐ and moisture‐resistant,
so water and fat are not able to leach through.
c15.indd 313 1/14/2016 7:37:10 AM
314 • CULINOLOGY®
Cereals and Baked Goods
Breakfast cereals are dry foods susceptible to moisture
absorption; therefore, they require good water vapor– and
fat‐barrier packaging such as coextruded polyethylene films
to help retain delicate flavors. Cereals are also susceptible to
oxidative rancidity, so antioxidants are commonly impreg-
nated into the plastic film lining ready‐to‐eat cereal cartons.
Soft baked goods such as breads, cakes, and pastries are
highly aerated structures and subject to dehydration and
relatively rapid staling (a starch crystallization process). In
moist environments, fresh bakery goods are also subject to
microbiological deterioration as a result of the growth of sur-
face mold and other microorganisms. Protective packaging
usually consists of simple monolayer polyethylene or ori-
ented polypropylene film pouches.
Hard baked goods, such as cookies and crackers, have a
relatively low water content and high fat content. Water can
be readily absorbed, and so the product loses its desirable
texture and becomes increasingly subject to lipid rancidity.
Packaging, such as oriented polypropylene film, is therefore
aimed at preventing moisture incursion.
Many salty snacks—corn chips, potato chips, roasted
nuts, and so on—are typically deep fat fried. These snacks
have very low water content (<2 percent) and, usually, a rela-
tively high unsaturated fat content (30 percent). Snack pack-
aging problems are compounded by salt, a catalyst for lipid
oxidation, and light, a catalyst for photo‐oxidation. In addi-
tion to lipid oxidation, such products are also subject to loss
of crispness due to moisture gain. Therefore, packaging strat-
egies include opaque packaging to prevent photo‐oxidation,
gas‐flushing with nitrogen to reduce oxygen, and packing the
product in hermetically sealed, gas‐ and water vapor–imper-
meable containers (pouches, canisters) to keep the nitrogen
in and oxygen and moisture out. Snack chips packed in flexi-
ble film bags are commonly pillow‐packed with excess nitro-
gen gas to provide extra protection against breakage.
FIGURE 15.9 Typical packaging for salty crack-
ers and analogous snacks. Interior coextruded
moisture‐barrier film for small unit portions
unitized in outer paperboard carton for protection
against physical damage during distribution.
wwing/Getty Images, Inc.
Candy
Chocolate, a high‐fat product, is sensitive to flavor change
and “bloom,” that is, whitening, if temperature is abused.
Inclusions such as nuts and fillings are susceptible to water
gain or loss. Chocolate is packaged in fat‐ and odor‐barrier
packaging to limit movement of fats in case of temperature
abuse, and prevent absorption of adverse odors from the
exterior environment. Aluminum foil is often used, but both
plastic films and plastic‐coated paperboards are adequate to
protect chocolate confections except against extreme tem-
perature abuse.
Sugar candies have very low moisture content (<1 per-
cent) and are susceptible to moisture gain. Packaging for
sugar candies must be highly moisture‐ and aroma‐resistant
to prevent moisture gain and consequent softening. Oriented
polypropylene film wraps are usually adequate for these
objectives.
Beverages
Beverages may be still or carbonated, alcoholic or nonalco-
holic. The largest unit volume of packages in the United
States is for two carbonated beverages: beer and soft drinks.
Both contain dissolved carbon dioxide, which delivers posi-
tive pressure within the package. With glass bottles, internal
carbonation is capable of stressing the closures; therefore
both screw and crown closures are employed to ensure
retention. With plastic bottles, the internal pressure is taken
advantage of to provide internal stress that helps maintain
package shape and permit thin‐walling the plastic for eco-
nomic and environmental reasons. Aluminum cans are also
able to be thin‐walled because the internal carbonation pres-
sure maintains the can shape. When the internal product
contents are not inherently carbonated, as with bottled water
or juice, nitrogen is injected to provide counter‐pressure and
thus maintain package shape.
Beer is more sensitive than other carbonated beverages
(or almost any other product) to oxygen, loss of carbon diox-
ide, off flavors, and light. Most, but not all, U.S. beer under-
goes thermal pasteurization after sealing in the package.
Thus, the internal pressure within the package can build to
FIGURE 15.10 Aluminum bottles of carbonated
beverages. Internal carbonation helps maintain
bottle shape.
jfmdesign/Getty Images, Inc.
c15.indd 314 1/14/2016 7:37:30 AM
CHAPTER 15 • Food Packaging • 315
FIGURE 15.11 Amber glass beer bottles in
paperboard multi‐packer. Amber color retards
biochemical changes due to light sensitivity.
Paperboard is opaque and adds to light blocking.
vicm/Getty Images, Inc.
well over 100 psi at 145°F (63°C), the usual pasteurization
temperature. If not carefully controlled, internal pressure
is capable of blowing off closures or distorting aluminum
cans. Much beer is packaged under excess carbon dioxide
to eliminate as much oxygen as possible and thus retard
oxidation of the contents. Other measures taken to reduce
oxidative deterioration include refrigerated distribution and,
for plastic bottles, incorporation of oxygen scavengers in the
package walls.
Package Materials
Packaging consists of a limited number of relatively inex-
pensive materials: paper, paperboard, aluminum, steel, glass,
plastic, and, less often, wood.
Paper, Paperboard, and
Corrugated Fiberboard
Paper and paperboard are the most common package mate-
rials in quantity and value, representing about 35 percent of
total packaging volume. They are used to make folding car-
tons and corrugated fiberboard distribution cases, sleeves,
pads, and so on, and have been employed since the nine-
teenth century. Virgin paperboard is derived directly from
wood and is therefore the strongest board. Often, but not
always, it is bleached or otherwise colored white for appear-
ance and printability. Because of its purity—that is, absence
of contaminants—virgin paperboard is often used for direct
food contact in food packaging and in foodservice take‐away
applications.
Corrugated fiberboard is a paper‐based material consisting
of a fluted fiberboard sheet with one or two flat linerboards
attached to either face. It is widely used in the manufacture of
corrugated and shipping containers. Corrugated fiberboard
was invented in England in the 1850s as an ingenious method
that transformed flimsy sheets of paper into a rigid, stack-
able, and cushioning form of packaging for delicate goods
in transit. Used to make shipping boxes since the 1890s,
this material has not changed significantly in over a
hundred years.
Old corrugated containers are an excellent source of
fiber for recycling. They can be compressed and baled
for cost‐effective transport. The baled boxes are put in a
hydropulper, which is a large vat of warm water for clean-
ing and processing the fiberboard. The pulp slurry is then
used to make new paper and fiber products. Recycling
corrugated fiberboard helps countries without sustain-
able wood resources build a paper and packaging industry
locally.
Recycled paperboard is derived from previously used
paper, such as newspapers or paperboard, corrugated fiber-
board, and clippings and cuttings from paperboard carton
or case converting plants. Much of the raw material is
industrial or post‐consumer waste from homes and offices.
Properties are reduced due to shortened, damaged fibers.
Recycled paperboard is potentially hazardous to food con-
tents because of intentional and unintentional additives
(inks, coatings, and so on) during previous use. Recycled
paperboard can have a good white printing surface if it is
clay‐coated on the paper‐making machine. Coated recycled
paperboard can be used for secondary packaging of cer-
tain low‐moisture foods such as cookies and crackers, cake
mixes, and breakfast cereals, where it does not come into
direct contact with the food.
Metal
Metal comprises about 130 billion cans, including 105 billion
two‐piece aluminum beverage cans (for beer and carbonated
beverages and juices), as well as caps, lids, and closures for
bottles and jars, and foil for flexible laminations. It has been
used since the early nineteenth century.2
Aluminum, used in thin‐walled cans for beer and soda,
is the most widely used metal for packaging. It is also used
in still beverage cans ( juices, fruit beverages, tea, and so
on) with the addition of inert gas—that is, nitrogen—for
pressurization. Aluminum foil is used in trays and pans
(pie tins, muffin tins, foil roasting pans, and so on), wraps,
flexible packaging, and multi‐material laminates. It is rela-
tively easy to fabricate, and the wall thickness can be
reduced to save weight. Metal closures are mechanically
seamed to the can bodies. Heat‐seal metal closures are
also available.
Steel is used mainly in cans for retort processing, almost
always internally coated with heat‐resistant plastics to pre-
vent the food from interacting with the steel and generating
metallic off flavors. Steel cans are made in two pieces or,
sometimes, three, with welded side seams. Mechanical double‐
seaming is the common closure technology.
c15.indd 315 1/14/2016 7:37:38 AM
316 • CULINOLOGY®
Glass
Glass bottles and jars for foods and beverages are the oldest
of the man‐made packaging materials, and the heaviest and
most energy intensive. Glass is inert, transparent, and an
excellent barrier, but relatively fragile. It requires a closure,
usually of a different material. It is declining in importance
as a package material to fewer than 40 billion bottles and
jars annually due to shipping weight and expense as well
as breakability.
Plastics and Plastic Packaging
Plastic, about 80 years old as a package material, is the young-
est family of packaging materials and the most versatile, least
expensive, and by far the most effective per unit of weight.
There are many types of plastics used in food and beverage
packaging, too many to adequately discuss all of them here,
but these are the most common.
Polyolefins
Polyolefins are composed of hydrocarbons extracted from
petroleum or natural gas. These include polyethylene (PE),
which is an excellent moisture barrier, and the most widely
used packaging plastic. In film form, it is used in myriad
applications, depending on its density: to contain bread, hard
baked goods, and candies and as a liquid‐barrier heat‐seal
coating on other plastics and on paperboard. It is made in
many varieties that differ in density and cross‐linking. These
three are the most common:
FIGURE 15.12 Two‐piece steel can with easy‐
open steel end. Paper label. Can is engineered
for high‐temperature, high‐pressure retorting to
sterilize the contents for ambient temperature
distribution.
Larry Herfindal/Getty Images, Inc.
• Low density (LDPE)—0.09 to 0.91 g/cm3: plastic wrap,
plastic bags, coated on paperboard to manufacture milk
and juice cartons.
• High density (HDPE)—0.95 g/cm3: milk jugs, margarine
tubs, bottles and bottle caps.
• Linear low density (LLDPE)—0.915 to 0.925 g/cm3: has
higher tensile strength and higher impact and puncture
resistance than does LDPE. It is very flexible and elon-
gates under stress. It can be used to make thinner films,
plastic wrap, stretch wrap, pouches, bottles, and jars,
and as a coating for other substrates, according to the
Modern Plastics Encyclopedia.
Rugged, translucent, reusable plastic storage containers
made in a wide variety of shapes and sizes for home use by
various companies (such as Rubbermaid and Sterilite) are
commonly made of high‐density polypropylene, although
the lids are often made of somewhat more flexible LDPE so
they can snap onto the container to close it.
Polypropylene (PP) is also a polyolefin moisture barrier
plastic fabricated into clear films and some bottles (not trans-
parent). As a film material, it is an excellent water‐vapor
barrier with transparency. It is widely used as a water vapor–
barrier film for snacks, hard baked goods, candies, and so on.
Food containers made from PE do not soften in the dish-
washer and do not melt during hot filling processes. For this
reason, most plastic tubs for dairy products are polypropyl-
ene sealed with flexible aluminum foil (both heat‐resistant
materials). Polypropylene can also be molded into disposable
bottles to contain liquids, powders, or similar consumer products.
Polyvinylidene chloride (PVDC, also known as Saran
wrap) is an excellent water‐vapor, fat, odor, and oxygen bar-
rier. It is used in laminations for an oxygen barrier in retort
package lidding materials. Polyvinylidene chloride is applied
as a water‐based coating to other plastic films, such as biaxi-
ally oriented polypropylene (BOPP) and polyester (PET). This
coating increases the barrier properties of the film, reducing
the permeability of the film to oxygen and flavors and thus
extending the shelf life of the food inside the package.
Ethylene vinyl alcohol (EVOH) is an excellent oxygen
barrier material that is moisture susceptible and so must be
protected, usually by lamination or coextrusion with poly-
propylene film. It’s probably the most widely applied oxygen
barrier material used today, found in almost all barrier
retort cans, bowls, and trays.
Polyester
Polyester is a category of plastic polymers that contain the
ester functional group in their main chain. Depending
on the chemical structure, polyester can be a thermoplastic
or thermoset; however, the most common polyesters are
thermoplastics.
Thermoplastic materials are those that can be melted and
resolidified without degradation. Most plastics used for food
and beverage applications are thermoplastics. Thermosets
harden when heated and set into a solid that does not remelt
without degradation.
c15.indd 316 1/14/2016 7:37:45 AM
CHAPTER 15 • Food Packaging • 317
Polyethylene terephthalate (PET) is the most common
thermoplastic polymer resin of the polyester family and is
used in fibers for textiles (most famously, “Dacron Polyester”),
containers for liquids and foods, and many other commercial
applications.
Because PET is an excellent water and moisture barrier
material, plastic bottles made from PET are widely used for
water and soft drinks. In this form, it is the most widely recy-
cled of all the plastics simply due to the enormous number of
PET bottles in use in the beverage industry, which ultimately
become part of the solid waste stream. PET is also used in
flexible food packaging, and if crystallizable PET is used, the
trays can be used for frozen dinners, since they withstand
both freezing and oven baking temperatures. Lightly metal-
lized PET film is used in microwave susceptor constructions
for browning and cooking food in microwave ovens.
Polystyrene (PS) for food packaging may be foamed
(expanded) to produce a very lightweight, yet fairly strong
material. PS is used extensively for supermarket trays for meat
and produce and for restaurant take‐away food packaging
(opaque foam “clamshells”). It is also widely used in hot drink
cups and egg cartons. Solid polystyrene may be oriented for
strength and transparency and may be blended with rubber to
offer a readily formable non‐barrier container, such as a cup or
bowl. It has little oxygen or water vapor barrier capability and
therefore is used mainly as structural material.
FIGURE 15.13 Expanded polystyrene thermo-
formed clamshell for QSR take‐away food for
short‐term thermal insulation.
travellinglight/Getty Images, Inc.
The majority of nylon (polyamide) is used as a component
of coextruded or laminated multilayer films for packaging of
foods sensitive to oxygen, such as fresh and processed meats,
smoked fish, cheeses, and prepared meals. Nylon films are
strong and puncture‐resistant; they have high barrier proper-
ties against oxygen, carbon dioxide, and many aroma chemi-
cals; and they have superior elastic properties.
Polyvinyl chloride (PVC) is not used as much as it could
be in food packaging applications because of the potential
toxicity of its chemical constituents, which include chlorine,
phthalates (for softening; not used in rigid PVC such as that
used for plumbing pipes and vinyl siding), and lead. Some of
these constituents have been shown to leach out of the plas-
tic and into the environment, where they may pose health
hazards. In addition, when incinerated, PVC creates dioxins,
which have been classified as carcinogens. This limits its abil-
ity to be recycled, and in fact it is the least recycled of all of
the plastics.
That said, for more than 40 years PVC film has been used
as stretch or cling film for overwrapping fresh meat, poultry,
and produce trays in retail stores to provide a transparent,
low‐moisture‐permeable, high‐gas‐permeable material that
can retard respiratory anaerobiosis in produce and provide
adequate oxygen to retain the red color of fresh meats. For
food catering and foodservice applications, PVC is still pre-
ferred because its stretchability and cling are much better
than common consumer wraps.
One of the thermoset materials is the epoxy amine made
from BPA (bisphenol A). Bisphenol A is used primarily to
make plastics (particularly polycarbonate plastic) and epoxy
resins. Products using bisphenol A have been in commercial
use since 1957. BPA‐based plastic is clear and tough, and is
made into a variety of common consumer goods, such as the
5‐gallon water bottles used on office water coolers, sports
equipment, CDs, and DVDs. Epoxy resins containing BPA are
used to line water pipes, and as coatings on the inside of most
food and beverage cans and the lining of metal bottle caps to
protect the food from direct contact with the metal.
BPA exhibits hormone‐like properties that raised concern
about its suitability in some consumer products and food con-
tainers, in particular baby bottles, baby food containers, and
“sippy cups.” Since 2008, several governments have investi-
gated its safety, and the publicity that followed prompted
many retailers to withdraw polycarbonate baby products and
water bottles. In 2012 the FDA ended its authorization to use
BPA in baby bottles and infant formula packaging, based on
market abandonment, not for health concerns (which the
FDA still feels have not been proven). The European Union
and Canada have banned BPA use in baby bottles as well. Also,
due to BPA health concerns in Japan, epoxy coatings in food
and beverage cans have been mostly replaced by PET film.
SPI: The Plastics Industry Trade Association represents
the entire plastics industry supply chain in the United States,
including processors, machinery and equipment manufac-
turers, raw materials suppliers, and recyclers. The SPI Resin
Identification Coding System is a set of symbols placed on
plastics to identify the polymer type. It was developed by SPI
c15.indd 317 1/14/2016 7:37:53 AM
318 • CULINOLOGY®
in 1988 and is used internationally. The primary purpose
of the codes was to make separation of the many similar‐
appearing plastic container types easier for recycling centers
across the country. Separation must be done carefully because
each type of plastic must be recycled separately. Even one
piece of the wrong type of resin can ruin a mix.
The symbols used in the code consist of arrows that cycle
clockwise to form a rounded triangle surrounding a number,
often with an acronym representing the plastic below the tri-
angle. These codes do not indicate how difficult the item is to
recycle, nor how often the plastic may be recycled. It is an arbi-
trarily agreed upon code that has no other meaning aside from
identifying the polymer used to manufacture each specific
plastic in order to facilitate future recycling of the plastic.
Use of the recycling symbol in the coding of plastics has
led to ongoing consumer confusion about which plastics are
readily recyclable. In many communities throughout the
United States, PETE and HDPE are the only plastics collected
in municipal recycling programs. Some regions, though, are
expanding the range of plastics collected as markets become
available. Los Angeles, for example, recycles all clean plastics
regardless of resin type. That said, plastic films and wraps are
rarely recycled due to the difficulty in collecting and separat-
ing them from other trash.
Package Testing
Principles and Protocols
Laboratory testing for flexible raw materials and finished pack-
ages is performed to gain information for use in remediation or
correction of problems and for optimizing the quality of pack-
age materials and finished structures. Chemical and biological
interactions of packaging and food—permeation (passage of
gases through the apparently solid walls of the container), and
transmission (entry through minute openings such as the seal
area of the package structure)—are measured instrumentally
and objectively. This includes real‐time testing, which can be up
to one year, and accelerated shelf‐life testing (ASLT), where test-
ers increase package storage temperatures to hasten the end of
product life. This simulates real‐time testing in a much shorter
timespan and allows fairly good predictability of actual shelf life.
The goal is to evaluate changes to the package’s contents during
shelf life because maintaining the eating quality of the food con-
tents is the real objective of any and all packaging. Evaluations
are conducted through consumer sensory testing, analytical
testing, and physical and chemical observations. Sensory test-
ing, when performed objectively, is often the best measure of
food quality. Materials are tested for properties such as:
Resin
Code
Polymer Name &
Abbreviation
Original Uses Recycled Uses
Polyethylene
terephthalate
(PET or PETE)
Water and soft
drink (soda) bottles
Mouthwash bottles
Peanut butter jars
“Polar fleece”
Car bumpers
Luggage
High-density
polyethylene
(HDPE)
Milk jugs
Margarine tubs
Yogurt containers
Playground equipment
Floor tiles
Laundry detergent
bottles
Polyvinyl chloride
(PVC or V)
Shampoo bottles
Medical tubing
Windex® bottles
Plastic decking
Mud flaps
Speed bumps
Low-density
polyethylene
(LDPE)
Squeeze bottles
Shopping bags
Carpeting
Trash-can liners
6-pack rings
Shipping envelopes
Polypropylene
(PP)
Straws
Medicine bottles
Syrup bottles
Car window ice
scrapers
Bike racks
Lawn rakes
Polystyrene
(PS)
Vending cups
Egg cartons
Styrofoam
Light switch plates
Packing peanuts
Insulation
Polycarbonate,
acrylic, nylon,
fiberglass,
barrier plastics,
other (“O”)
5-gallon water
bottles
CDs and DVDs
Sunglasses
Plastic decking
Plastic benches
1
2
3
4
5
6
7
FIGURE 15.14 The SPI Resin Identification Coding System.
Source: J. J. Cousminer
c15.indd 318 1/14/2016 7:37:54 AM
CHAPTER 15 • Food Packaging • 319
• Permeability—of gases, moisture, and water vapor
• Strength—tearing, puncture, impact, compression
• Gauge—size or thickness
• Odor barrier—effectiveness
Conditioning of the materials to uniform predefined mois-
ture and temperature conditions is often performed before
testing to minimize external environmental variables.
Filled production or test packages are evaluated for:
• Seal and closure strength
• Transmission of gas, water vapor, and odors
• Vibrations to simulate travel (road, rail, ocean, air)
• Impact—drop test
• Compression resistance—stacking in warehouse and on
trucks or rail cars
• Where appropriate, time for product to reach unaccept-
ability as determined by sensory, chemical, or micro-
biological testing
Economics
Package economics requires understanding the total system—
materials, labor, production, and distribution—to measure the
actual cost of packaging. Generally, the total cost of packaging is
about 7 percent of the final retail price, but this is a general aver-
age for all foods and beverages. Total cost of packaging includes:
• The primary package—that is, the individual container
(tray, bottle, jar, can)—with which the food or beverage is
in direct contact. Also, the lid, cap, film, or other closure.
• The overwrap or shrinkwrap, if applicable.
• The label, sleeve, or outer box that goes around the inner
package. This is where the product identification, pack-
age graphics, ingredient statement, nutritional facts
panel, net weight statement, preparation instructions,
health and nutrition claims (if any), UPC code, and name
of producer are located.
• Secondary packaging, also called unitizing packaging,
which holds a set number of individual units to be sold as
a group—for example, six‐pack folding cartons for beer.
• Distribution packaging—that is, outer cases (often fiber-
board)—used to pack multiple primary or secondary
packages (12‐packs, 24‐packs, and so on).
• Label stock for the secondary packaging. Also packing
tape or glue to seal the cases, and stretch film to secure
multiple cases to a pallet for shipping.
• The pallet.
These are general requirements. Variations occur with
each product being packaged.
Environmental Impact
Municipal solid waste (MSW)— commonly known as trash
or garbage—consists of everyday items people use and then
throw away, such as product packaging, grass clippings,
furniture, clothing, bottles, food scraps, newspapers, appliances,
paint, and batteries. This waste comes from homes, schools,
hospitals, and businesses. In 2013, Americans generated
about 250 million tons of trash.3
Packaging of all kinds, including paper and paperboard
(not just food packaging), constitutes 25 to 30 percent of
municipal solid waste. Of this, one of the largest volumes is
retail packaging, such as expanded polystyrene from take‐
out food and supermarket delicatessens, but this is still only
a fraction of total solid waste. Waste disposal strategies are
critically important, and actions in this highly emotional
issue should be carefully considered.
A company can choose source reduction, which reduces
the quantity of packaging used—already a continuing goal
of converters and packaging firms, for economic reasons.
Biodegradable packaging is not yet truly effective or commer-
cially feasible but is attractive because it seems so simple.
Starch‐based package materials continue to be studied; some
have been commercialized. Polylactic acid (PLA) is a thermo-
plastic derived from cornstarch that has been commercial-
ized as a selectively compostable package material.
Incineration waste‐to‐energy is effective in Japan and
other countries, and where used in the United States. Trash,
including paper and paperboard and plastic materials, is
combusted to produce energy that generates electric power
in waste‐to‐energy plants.
Dumping is strictly controlled in the United States because
of problems with polluting the water supply and with reduced
land area available for landfill. Sanitary landfills are now
mandated to ultimately become parkland or building sites.
Among the potential usable by‐products of sanitary landfills
are gases from anaerobic decomposition of waste that can be
burned for energy or for production of electricity.
Recycling is currently performed by almost all major pack-
age material suppliers for all materials in their own plants. The
economics of pre‐consumer waste, such as corrugated paper-
board and office papers, is often quite good, so these materials
are used in recycling plants as raw materials. Post‐consumer
waste recycling economics is generally poor because post‐
consumer wastes are an agglomeration of many materials that
must be precisely separated to not contaminate the recovery
streams. Nevertheless, this is possibly the best method in the
long run, as many communities are accustomed to the idea
of separating recyclables from garbage every week for curb-
side pickup.
Sustainable Packaging
Organizations are now being measured by shareholders and
independent agencies for their contributions to maintaining
the natural environment—that is, for their part in supporting
a sustainable planet.
Sustainable packaging is a new way of designing packag-
ing from the ground up that encompasses systems that do not
deplete the planet’s natural resources (oxygen, water, petroleum,
and plant life). Some components are based on new technology
(for example, using hybrid vehicles exclusively for distribu-
tion of materials), and some are based on updated versions of
c15.indd 319 1/14/2016 7:37:54 AM
320 • CULINOLOGY®
older technologies (solar power, wind power, geothermal power,
and so on). Inorganic packaging materials are designed to be not
only completely nontoxic and recyclable but also reusable, and
organic packaging materials are expected to be not just com-
postable but also to add nutrients to the soil. Sustainable sys-
tems minimize use of energy and aim to reuse all materials as
renewable resources. Benefits of sustainable packaging include:
• The safety and health of people and the environment
throughout the package’s life cycle.
• Meets market criteria for performance and cost.
• Packaging is sourced, manufactured, distributed, and
recycled using renewable energy.
• Takes advantage of clean production technologies.
• Maximizes use of renewable resources.
• Packaging is engineered to optimize materials and energy.
• Packaging materials are recovered and reused or recycled
using “cradle‐to‐cradle” rather than “cradle‐to‐grave”
mentality. Used packaging should not be buried; it
should be “resurrected.”
The transition to sustainable packaging requires many
changes because renewable energy and material sources are
not yet economically feasible in most countries, and it will be
some time before scientists develop the efficiencies neces-
sary for planetary sustainability.
Further Examples
of Current and Future
Trends in Food Packaging
Manufacturers and consumers are dictating closer ties
between technology and marketing, demanding better prod-
uct safety and quality closer to fresh or freshly prepared.
Current trends include:
• Expansion of the range of packaging materials made for
hot‐fill‐and‐hold processing beyond glass jars (current)
to pouches, plastic tubs, and trays.
• Development of retortable multilayer barrier plastic
cans, pouches, and trays—increasingly used for seafood,
poultry, rice, pastas.
• Evolution of an aseptic process and packaging for larger
particulate‐containing low‐acid foods such as chunky
soups and entrées. Storage and distribution may be refrig-
erated (extended shelf life) or ambient (shelf‐stable).
• Controlled or modified atmosphere packaging designed
for minimally processed foods (in particular, those that
must comply with the USDA’s requirement for “natural”),
now including micro‐oxygen packaging, where total
oxygen in the package is limited to less than 50 parts per
billion in prepared and packaged foods.
• Development of active packaging, which senses change
in the package environment and changes properties in
response. Oxygen scavengers, which remove residual
oxygen in a limited range of food and beverage packages,
are a subset of active packaging. Next generation:
odor‐removing packaging.
• Expansion of technology for self‐heating packages for bev-
erages and meals, which has been hampered by technical
issues. Such packaging is successfully used for military field
rations and a handful of specialty retail and camping prod-
ucts. Self‐cooling packages are under development.
• Perfection of packages that signal spoilage or even the pres-
ence of pathogens—these are highly publicized but not cur-
rently practical, as they generate too many false readings.
• Use of glass‐coated films in retort pouches that allow
visibility of contents.
• Integration of processing and packaging technologies
for improved health and safety—for example, metal cans
with inert plastic film coatings (instead of potentially
harmful chemical coatings) inside and out.
Conclusion
Packaging affects every step of the food production process,
from the farm, to the processor, to the market, to the restau-
rant, and, ultimately, to the consumer. It is critical for
Culinology® professionals to have a basic understanding of
packaging, where it comes from, what the packaging options
are, and how packaging is integrated with not just the inno-
vative food products they work to develop, but also the envi-
ronment we all live in.
Resources
Brody, Aaron L., and John Lord. Developing New Food Products for a Changing
Marketplace. 2nd ed. Boca Raton, FL: CRC Press, 2007.
Brody, Aaron L., and K. Marsh, eds. Encyclopedia of Packaging Technology.
2nd ed. New York: John Wiley & Son, 1997; 3rd ed. Hoboken, NJ: John Wiley
& Sons, 2010.
Robertson, Gordon. Food Packaging. 2nd ed. New York: Marcel, Dekker, 2006;
3rd ed. Boca Raton, FL: CRC Press, 2013.
Selke, Susan. Understanding Plastics Packaging Technology. Munich, Ger-
many: Hanser, 1997.
Selke, Susan, John Cutler, and Ruben Hernandez. Plastics Packaging. 2nd ed.
Munich, Germany: Hanser, 2004.
Soroka, Walter. Fundamentals of Packaging Technology. 4th ed. Niles, IL:
Institute of Packaging Professionals, 2010.
Periodicals:
• Brand Packaging
• Flexible Packaging
• Food and Beverage Packaging
• Package Design
• Packaging Digest, especially the Packaging Resources Book and Directory
• Packaging World
• Packaging Strategies
Other books, conference proceedings, and directories, including the
Package Machinery Manufacturers Institute (PMMI) Directory in
electronic format: www.pmmi.org.
1Van Arsdel, Cople, and Morgan (1973).
2http://www.compaxpackaging.com/packaging_history_metal.html
3U.S. Environmental Protection Agency, Municipal Solid Waste Generation,
Recycling, and Disposal in the United States: Facts and Figures for 2011
(May 2013).
c15.indd 320 1/14/2016 7:37:54 AM
http://www.pmmi.org
http://www.compaxpackaging.com/packaging_history_metal.html
16
CHAPTER 16 • Developing Nutritious Food Products • 321
Developing
Nutritious Food
Products
Lead Author: Darryl L. Holliday, Ph.D. , CRC®, Assistant Professor , Our Lady of Holy Cross
College
Margaret D. Condrasky, Ed.D , R.D., CCE, Associate Professor, Food, Nutrition, and Packaging
Sciences Department , Clemson University
Marie Hegler , Food Safety and Nutrition Agent, Cooperative Extension Service ,
Clemson University
Contributor: John W. Finley , Professor and Department Head of Food Science , Louisiana
State University
Introduction: Overview
of Health, Well‐Being,
and Healthy Eating
At its most basic, food is important to us because it delivers
the basic nutritional needs to sustain life. Its preparation
and eating rituals are ingrained in all cultures, it is central
to most social occasions, and, as a result, it is essential to our
lifestyles. Modern nutritional science has improved our under-
standing of the relationship between what we eat and our
health and wellness, both physically and emotionally. Concerns
related to the current U.S. obesity epidemic bring about ques-
tions of how much and what we eat, but the solution is not
simply less food and more exercise. The pleasures of eating
satisfying foods must also be factored in, along with an appro-
priate diet and exercise level, and that diet should include
a growing list of micronutrients that help protect us from
disease. The ideal healthy lifestyle should include a balance of:
1. Regular exercise
2. A healthy, balanced diet
3. Proper amount of rest
A healthy diet includes foods that provide adequate
energy and nutrients for us to thrive in mind, body, and spirit,
with occasional treats to provide pleasure. Foods must provide
sufficient amounts of proteins, fats, and carbohydrates while
being rich in the essential vitamins, minerals, and that most
easily overlooked nutrient: water . By integrating nutritional
principles and applications, we can create delicious, nutritious
dishes (and indulgent snacks and desserts) that inspire, tan-
talize, and, ultimately, satisfy, while also delivering foods that
enhance health and wellness. Culinology® is our means to
developing satisfying and pleasurable foods while supporting
good health and helping prevent chronic disease.
Our Changing Understanding
of Nutrition, Food, and Health
Modern research is ongoing in nutrition, food science, and medi-
cine to better understand how food and health are related. This
integrated approach yields frequently changing theories and
recommendations as new technologies emerge, often resulting
in confusion for the consumer. This also presents a challenge
for food and health professionals whose main focus is the ever‐
present need to meet the latest nutritional guidelines.
A perfect case in point is the latest revamp of the govern-
ment ’ s nutritional guidelines. After promoting various forms
of the food pyramid for decades, the U.S. Department of
Agriculture (USDA) has switched to a dinner plate icon. Visit
the USDA website for current information.1
That said, even a clear understanding of the latest data on
the impact of diet on health is not useful if it is ignored. For
the new recommendations to be of value, fresh and innova-
tive ways must be found to deliver them in easily accessible
foods (nutritious, tasty, affordable, and convenient) that can
be part of an improved diet. For more than 30 years, nutri-
tionists have recommended that we eat more fruits and veg-
etables, and yet statistics show that consumption has not
changed significantly during that time. 2 Many people either
do not like, cannot afford, or have limited access to fresh
c16.indd 321 1/22/2016 1:17:56 PM
322 • CULINOLOGY®
fruits and vegetables; therefore, they do not consume them.
This is just one of many challenges for today’s Culinology®
professional.
Basic Nutrition Principles
and Rationale
To design sound, nutrition‐based foods for a healthier diet,
we must examine the basic nutrients and their roles in food:
protein, lipids, carbohydrates, vitamins, minerals, phyto-
chemicals, and water. But first, we must look at the many fac-
tors that influence dietary decisions.
Calories
The first consideration is the energy content of foods, meas-
ured in calories (shorthand for kilocalories). Total calories in a
food or beverage are a measure of its energy content, which is
the total energy provided by the fats, protein, carbohydrates,
and alcohol in the food. The number of calories in a food is
measured by a calorimeter, which incinerates the food and
measures the heat given off. Using that measurement, an esti-
mation of how the body would utilize the food can be made,
resulting in a calorie designation in kilocalories (kcal). The
energy content of the macronutrients are as follows:
Fats: 9 kcal/g
Alcohol: 7 kcal/g
Protein: 4 kcal/g
Carbohydrate: 4 kcal/g
These values are based on each of these food components
being 100 percent digested, which isn’t always the case (for
example, resistant starches, a type of carbohydrate that
provides only 3 kcal/g).
Seventy percent of the energy in the diet is used for the
body’s basal metabolic requirements (BMR): temperature
maintenance, heartbeat, respiration, digestion, and so on.
The rest is either expended through exercise or stored in the
body as fat. As little as 100 extra calories per day can result in
the gain of 10 pounds in a year. Exercise notwithstanding, if
an individual consumes the exact amount of calories his or
her body requires, that person will maintain current weight.
Obviously, by consuming less than the calories expended, a
person loses weight.
Serving Size Versus Portion Size
Serving size is the unit of measure describing the average sin-
gle serving of a given food. With few exceptions, it appears on
the nutrition facts panel (NFP) of every packaged food prod-
uct sold in the United States. This is the standardized amount
of food eaten in one serving as specified by the USDA, and it
allows side‐by‐side comparisons within categories. That said,
it is important to carefully read the NFP on all packaged
foods because even similar‐looking foods (such as breakfast
cereals) may appear to have the same nutritional measure-
ments when in actuality these measurements are based on
different serving sizes.
FIGURE 16.1 Portion distortion.
20 Years Ago Today
Coffee
(with whole milk and sugar)
8‐ounce serving size
45 calories
Mocha
(with steamed whole
milk and syrup)
16‐ounce serving size
350 calories
Blueberry Muffin
1.5‐ounce serving size
210 calories
Blueberry Muffin
4‐ounce serving size
500 calories
Pepperoni Pizza
2 slices
500 calories
Pepperoni Pizza
2 slices
850 calories
Chicken Caesar Salad
1½‐cup serving size
390 calories
Chicken Caesar Salad
3½‐cup serving size
790 calories
Popcorn
5‐cup serving size
270 calories
Popcorn
11‐cup serving size
790 calories
Cheesecake
3‐ounce serving size
260 calories
Cheesecake
6‐ounce serving size
640 calories
Chocolate Chip Cookie
1½‐inch diameter serving size
55 calories
Chocolate Chip Cookie
5‐inch diameter serving size
275 calories
Chicken Stir Fry
2‐cup serving size
435 calories
Chicken Stir Fry
4½‐cup serving size
865 calories
Source: National Heart, Lung, and Blood Institute Obesity Initiative,
Portion Distortion II Interactive Quiz. Accessed at: http://hp2010
.nhlbihin.net/portion.
Foods that are measured in bulk, such as sugar, milk,
and cereal, are listed in common household measure-
ments like cups, tablespoons, and fluid ounces. Foods that
are usually divided to serve more than one person or for
more than one meal, such as pizza, cake, and pie, are listed
in fractional amounts like “1 4 pizza.” Also, foods that come
in individual units, such as slices of bread, potato chips,
and individual snack packets are listed as “15 chips” or
“1 package.”
Portion size is the amount of food a person chooses to
eat. For instance, you would eat more than the recommended
serving size if you ate a whole pizza rather than the quarter‐
pizza listed, or you would eat less if you ate ½ cup of cereal
rather than the 1 cup listed on the food label.
Portion Control
Over time, U.S. consumers have grown accustomed to eating
oversized portions. Portion sizes currently selected by adults
are not only larger than those established by the USDA but
c16.indd 322 1/14/2016 12:50:44 PM
http://hp2010.nhlbihin.net/portion
CHAPTER 16 • Developing Nutritious Food Products • 323
also significantly larger than portion sizes selected by young
adults two decades ago, according to a recent study in the
Journal of the American Dietetic Association.3 The researchers
found that portion sizes of “virtually all foods and beverages
prepared for immediate consumption” have increased over
the last two decades, as have portion sizes of individually
packaged and ready‐to‐eat prepared foods and items served
at fast‐food restaurants. The serving sizes set by the U.S.
government are much smaller than the actual portions served
in restaurants, and even in the home, meaning excess calo-
ries are routinely consumed. This is likely a major factor in
the obesity epidemic.
Chef’s Portioning Habits
In recent decades, the frequency of eating out and preva-
lence of obesity in all populations, groups, and ages have
increased dramatically, making the importance of control-
ling calorie intake in the restaurant setting more signifi-
cant. Chefs play an integral role in preparing and serving
healthful food. While surveys conducted by Clemson
University report that chefs recognize the importance of
nutrition in menu planning, many prepare meals that are
inconsistent with the U.S. Dietary Guidelines. Little is
known about chefs’ opinions or knowledge of ingredient
utilization regarding modification for healthy recipes or
about the role chefs play in determining portion sizes in
the United States.
Dr. Margaret Condrasky of Clemson University, in collabo-
ration with researchers from Pennsylvania State University,
conducted a study to determine the portioning habits of
chefs. A survey was distributed to 300 chefs to learn who
establishes restaurant portion sizes, factors influencing these
decisions, what food portion sizes are served in restaurants,
and chefs’ opinions regarding nutrition information and
weight management. Results identified executive chefs as
being most responsible for establishing restaurant portion
sizes, with presentation of foods, food cost, and customer
expectations as influential factors. However, 76 percent of
chefs thought they served “regular” portions when they actu-
ally served portions 2 to 4 times larger than serving sizes
recommended by the U.S. government.4
The study also found that while chefs believe the amount
of food served influences customer consumption and large
portions pose problems for weight management, their atti-
tudes varied regarding whether or not it is the customer’s
responsibility to eat a reasonable amount of food when
served a large portion. As portion size is a major determin-
ing factor of energy intake and the results of the study show
(1) customer expectations and (2) cost as the greatest
influences in determining restaurant portion sizes, strate-
gies are needed to encourage chefs to provide and promote
smaller portions more appropriate for customers’ energy
requirements.5
Energy Density
Energy density (ED) relates to the amount of energy ( calories)
per amount of food consumed. It is commonly expressed in
kcal/g.
kcal g ED/
Equal amounts of foods with higher fat content have
higher energy densities than foods with lower fat content. In
other words, foods higher in energy density, such as sweet
baked goods and fried foods, provide excessive calories per
gram consumed, while the same amount of foods lower in
energy density, such as water‐dense fruits and vegetables,
provide much lower calorie content. However, not all energy‐
dense foods are empty‐calorie foods. Nuts are energy‐dense
because of their high fat content, but they also provide pro-
tein, vitamins, and minerals. Increasing consumption of
foods with lower energy density, such as fruits and vegeta-
bles, and lowering fat intake can decrease caloric intake while
maintaining a sense of satiety.
Volumetrics
The Volumetrics Eating Plan developed by Barbara Rolls,
professor of nutritional sciences at Pennsylvania State Uni-
versity, focuses on reducing the energy density of the diet
using the latest research on controlling hunger while manag-
ing calories.6 Rather than eating smaller portions of energy‐
dense fattening foods, eating larger portions of foods with
lower energy density (such as fruits and vegetables) enhances
the feeling of fullness and decreases the feeling of being
shortchanged.7
With consumers expecting more food for less money, the
food industry may find it difficult to sell reduced portions
without drastically reducing price. This makes knowledge
and know‐how about energy density important in healthy
new food product development. As a research chef or prod-
uct developer with the goal of customer satisfaction, how do
you decrease calories per bite while maintaining satisfying
portions? Create foods high in water content and fiber and
low in fat and calorie density, such as those that are fruit‐ and
vegetable‐based, plus brothy soups, lean meats, and low‐fat
dairy products. Here is a list of ways to reduce calories per
bite, adapted from Volumetrics:8
More Fiber
• Add beans, lentils, and peas to soups and salads.
• Use high‐fiber vegetables, like carrots and broccoli, as
side dishes or additions to salads and soups.
• Replace white rice, bread, and pasta with brown rice,
whole‐grain products, whole wheat pasta, and exotic
grains like bulgur or amaranth.
• Substitute almonds or other nuts for croutons in salads.
• Experiment with international dishes that use whole
grains, such as tabouli, or lentils, such as Indian dals.
• Use whole fruits and vegetables. Most of the fiber is in
the skin or peel.
c16.indd 323 1/14/2016 12:50:44 PM
324 • CULINOLOGY®
• Cook vegetables briefly because the longer they cook,
the more fiber they lose. Try steaming them until they
are crisp yet tender to retain most of the fiber content.
• Use oatmeal, whole wheat flour, brown rice, or wheat
germ in baked goods.
More Lean Proteins
• Protein‐rich foods are particularly satiating because
they control hunger when calories are restricted.
• Choose high‐protein foods of low energy density and
cooked with little added fat, such as lean steak, pork ten-
derloin, skinless chicken or turkey breast, fish, shellfish,
egg whites, beans, and tofu.
• Don’t overlook vegetable sources of high‐quality protein,
such as soy, quinoa, and complementary combinations
of grains and legumes; these also provide fiber, vitamins,
minerals, and an assortment of phytonutrients.
Nonfat or Low‐Fat Dairy
• Most dairy products are available in low‐fat or nonfat
versions.
Use Low‐Sodium Stocks, Vegetable Juice, or Water
• Soups based on broth, rather than cream or a starchy
puree, are very low in energy density and provide a satis-
fying larger portion with fewer calories.
• Water, which lowers the energy density of a food, can be
incorporated into foods to increase volume and enhance
satiety.
Add Flavor Rather than Sodium
• Fresh herbs, spices, garlic, vinegars, and citrus juices
enhance flavor without adding sodium or calories.
Eat More Vegetables and Fruits
• Add fruits to side dishes and desserts.
• Add vegetables such as zucchini, yellow squash, peppers,
onions, eggplant, and spinach to pasta dishes and pizza.
• Increase the proportion of vegetables in stir‐fry dishes,
fajitas, soups, and stews.
The Food Guide Pyramid
In 1943, the USDA released the first Food Guide Pyramid,
which grouped foods into seven categories. In the mid‐1950s,
the pyramid was simplified into four food groups: milk,
meat, fruit and vegetable, and bread and cereal. In 1979, the
USDA issued the “Hassle‐Free Guide to a Better Diet,” which
included a fifth food group for fats, sweets, and alcoholic bev-
erages. The Food Guide Pyramid depicted in Figure 16.2 was
introduced in 1992 as a revamped addition of the “Hassle‐
Free Guide.”
Bread, Cereal,
Rice, & Pasta
Group
6-11
SERVINGS
Fruit
Group
2-4 SERVINGS
Meat, Poultry, Fish,
Dry Beans, Eggs,
& Nuts Group
2-3 SERVINGS
Vegetable
Group
3-5 SERVINGS
Milk, Yogurt,
& Cheese
Group
2-3 SERVINGS
Food Guide Pyramid
A Guide to Daily Food Choices
Fat (naturally occurring
and added)
Sugars
(added)
These symbols show that fat and added
sugars come mostly from fats, oils, and
sweets, but can be part of or added to
foods from the other food groups as well.
KEY
Fats, Oils, & Sweets
USE SPARINGLY
FIGURE 16.2 Food Guide Pyramid.
Source: U.S. Department of Agriculture/U.S. Department of Health and Human Services.
c16.indd 324 1/14/2016 12:50:45 PM
CHAPTER 16 • Developing Nutritious Food Products • 325
MyPyramid and MyPlate
In 2005, the USDA released the MyPyramid Plan based on the
Dietary Guidelines for Americans.9 MyPyramid was a refine-
ment of earlier food guide pyramids, which incorporated the
latest dietary recommendations of the USDA. It could be tai-
lored to fit most people based on characteristics such as age,
gender, level of activity, and pregnancy status. Additional cus-
tomization, which was available online, allowed people to
adjust their diet for weight loss, maintenance, or gain. The
silhouette of a person walking up steps represented two con-
cepts: (1) the need for physical activity, and (2) the steps to a
healthier individual. Lastly, food categories were presented in
six vertical bands: grains, vegetables, fruits, oils, milk, and
meat (including red meat, poultry, and seafood) and beans,
with a different color marking each. The bands were thicker
on the bottom and thinner on top to show that there are
healthier food choices within each category, and they were
different widths to illustrate the relative amounts of foods in
each category that should be eaten.
In 2010, after 67 years of food pyramids, the USDA
decided there was a better graphic to illustrate the latest
scientific findings on the ideal diet and came up with
MyPlate. They also simplified the overall recommenda-
tions to the following:
• Fill half the plate with fruits and vegetables.
• Divide the other half between grains and proteins.
• Add a glass of dairy.
• Eat less, and avoid oversized portions.
• Increase consumption of fruits and vegetables.
• Make half of carbohydrate consumption whole grains.
• Drink low‐fat (1 percent) or nonfat milk.
• Reduce consumption of processed foods with high
sodium levels.
• Drink water instead of sugary drinks.
Drilling down yields additional recommendations:
• Choose lean meats and poultry, trim off excess fat and
skin, and cook without added fat or breadings.
• Choose seafood as the main protein at least twice a
week. Look for seafood rich in omega‐3 fatty acids, such
as salmon, trout, and herring.
• Choose beans, peas, or soy products as a main dish or
part of a meal often.
• Oils are not a food group, but they do provide essential
nutrients in the diet. The monounsaturated fatty acids
(MUFAs) and polyunsaturated fatty acids (PUFAs) found
in fish, nuts, and vegetable oils do not raise LDL (bad)
cholesterol levels in the blood. In addition to the essen-
tial fatty acids they contain, oils are the major source of
vitamin E in the typical U.S. diet.
• While some oil is needed for health, oils are quite caloric
(9 kcal/g). Therefore, limit the amount of oil consumed
to balance total calorie intake. Most individuals should
limit their intake to the equivalent of 2 tablespoons of oil
(28 g) per day.
• Physical activity and nutrition work together for better
health. Being active increases the amount of calories
burned. As people age, their metabolism slows, so main-
taining energy balance (and body weight) requires mov-
ing more and eating less.
FIGURE 16.3 MyPlate.
Source: U.S. Department of Agriculture/U.S. Department of Health and Human Services.
c16.indd 325 1/14/2016 12:50:46 PM
326 • CULINOLOGY®
Many more recommendations are at the USDA MyPlate
website.10
Dietary Guidelines and Portion
Recommendations
The Dietary Guidelines for Americans, of which MyPlate is a
part, are published jointly every five years (since 1980) by the
U.S. Department of Health and Human Services (HHS) and
the USDA. The Guidelines provide authoritative advice about
how good dietary habits can promote health and reduce risk
for major chronic diseases. These recommendations serve as
the basis for federal food and nutrition education programs.
It is advisable to check the latest edition of the Guidelines
(2010) on the USDA website for the most up‐to‐date
information.11
More Matters®
Developed by the Produce for Better Health Foundation
(PBH) in partnership with the Centers for Disease Control
and Prevention (CDC), Fruits and Veggies—More Matters® is
a national public health initiative created to encourage
Americans to eat more fruits and vegetables—fresh, frozen,
canned, dried, and 100 percent juice. The initiative is a
national call to action that is attainable and easy for people to
understand: simply, eat more fruits and vegetables. More
than 90 percent of U.S. residents consume fewer fruits and
vegetables than the daily amount—3½ to 5½ cups, depend-
ing on age and gender—recommended by the Dietary
Guidelines for Americans.
When it comes to good nutrition, all forms of fruits and
vegetables matter. With numerous options and conveni-
ent packaging that makes fruits and vegetables easy to
store and serve, eating the recommended amount is easier
than ever.
• Most frozen and canned foods are processed within
hours of harvest, so their flavor and nutritional value are
preserved.
• Studies show that recipes prepared with canned pro-
duce have similar nutritional values to those prepared
with fresh or frozen ingredients, although consumers
should be aware that canned vegetables often contain
elevated levels of sodium from salt added during proc-
essing, and should compensate by reducing the addition
of extra salt on the stove or at the table.12–14 The same can
be said for sugar or syrups added to canned fruits. Read
and understand nutrition statements!
• Canned foods are cooked before packaging, so they are
recipe‐ready.
• Frozen foods also require little preparation—washing
and slicing, for instance, is already done.
Many people struggle to consume the necessary servings
of vegetables because of price, availability, flavor, required
prep steps (washing, trimming, peeling, cutting, cooking, and
so on), or a combination thereof. In many cases, meats, espe-
cially high‐fat varieties, are less expensive, easier to prepare,
and more filling. To motivate at‐risk individuals to follow a
more vegetable‐centric diet plan, researchers from Louisiana
State University are combining their knowledge of food sci-
ence, culinary arts, and nutrition to create ingredients and
meals that will lead to increased vegetable consumption. The
meals use vegetable‐based meat, poultry, and seafood extend-
ers to decrease fat while adding fiber to hold moisture in
reformed products such as patties, nuggets, links, and
restructured and reshaped seafoods. These technologies are
designed to offer the consumer a tasty, low‐cost, more appeal-
ing, and more easily accessible source of fruits and vegeta-
bles. Within the Food Science and Human Nutrition
department at Clemson University, research, education, and
outreach efforts are coordinated within the CU CHEFS®
instructional program. New product development and nutri-
tion educator teams engage the community and foodservice
industry in healthy menu creation.
Nutritional Analysis Software
Nutritional analysis software is a valuable tool in creating
high‐quality, nutritionally appropriate food products and
package labels. Available software programs range from very
expensive, covering a giant database of food items, to essen-
tially free, covering a more moderate selection of food items
and nutrients. Depending on your company’s needs and the
range of ingredients you are working with, custom software
can also be created.
Nutritional development software has pushed product
development and nutrient analysis to new levels of ease and
accuracy. They enable you to customize your work and save
countless hours of data entry and hand calculations. In addi-
tion to the many label formats, numerous professional reports
are available. Many allow the user to view the analysis as a
spreadsheet, protein quality report, bar graph, single nutrient
report, and many, many others. The cost for these programs
depends on the software manufacturer, the number of users in
a given company who will have access to the program, and
whether they are installed onto a single computer, a corporate
network, or accessed from a cloud network.
Product features can include:
• Child nutrition
• Supplement facts panel
• A meticulously researched database of more than 37,000
foods and food items, including raw materials, chemicals,
and food industry ingredients. Users can add an unlimited
number of new foods and modify existing foods.
• An intuitive, user‐friendly interface that uses the latest
in ingredient search technology
• Automatic nutrient analysis for 160+ nutritional compo-
nents, including food exchanges
• Labels: United States, standard, tabular, dual declara-
tion, aggregate, and more (including Canadian, Spanish,
and bilingual formats)
c16.indd 326 1/14/2016 12:50:46 PM
CHAPTER 16 • Developing Nutritious Food Products • 327
• Trans fat data and label display option
• Automatic ingredient statements, allergen statements,
and nutrient content claims
• Cost entry and calculations
• Fat formulator, which calculates approved data for
meat‐to‐fat ratios and custom‐blend products
• Moisture and fat recipe adjustments: Subtract or add
water or fat, view results on screen immediately.
• Yield adjustments and tables: Enter food “as purchased,”
obtain nutrient values for edible portion only.
• Cloud access
While there are several excellent and quite sophisticated
nutritional software programs available to rent or purchase,
Gnutrition is a free nutrition analysis software program that
uses the USDA Nutrient Database of Standard Reference,
Release 13, 1999, as the source of food nutrient information.15
It contains data on 81 nutrients for more than 5000 foods,
which is significantly less than the paid versions but serves
the basic needs of its users.
Gnutrition offers the following features:
• The ability to search the database for a specific food and
examine its nutrient composition.
• The ability to create a recipe and to compute its nutri-
tional composition, which can be compared to the users’
own dietary goals.
• A searchable database of all the created recipes.
• The ability to record and plan meals day by day. The
average and total nutrient intake can be computed over
any given period.
• The MySQL database system, used to store the data.
Ingredients: Nutrition
and Function
Macronutrients (fats, protein, carbohydrates, and water) are
the primary factors that determine the physical state and
structure of foods. These rarely act independently. For
example, it is the interaction of water, gluten protein, and
pentosan polymers (structural carbohydrates) that deter-
mine the function of bread dough. Understanding the pri-
mary structures and functions of each macronutrient helps
one appreciate their more complex interactions in real food
systems.
The need for macronutrients in the diet can be very
different for individuals depending on age, activity, and lifestyle;
however, general ranges are recommended.16 Total calories in
the diet should be 45 to 65 percent carbohydrates, 10 to 35
percent protein, and 20 to 35 percent fats. Saturated fats
should comprise less than 10 percent of total energy intake;
therefore, a person consuming a 2000‐calorie diet should take
in only 200 calories (or about 22 g—about ¾ of an ounce) of
saturated fat. Cholesterol intake should be limited to less
than 300 mg (1/100th of an ounce, or roughly the amount in
2 egg yolks) per day.
Protein
Proteins are chemically the most complex of the food macro-
nutrients, being composed of up to 21 amino acids bound
together in polymers (long chains) that can range from a few
amino acids to several thousand. Molecules of between 2 and
50 amino acids are called peptides; when the number of
amino acids is above 50, these molecules are named proteins.
Proteins can provide energy to the body (4 kcal/g), but, more
importantly, they are the building blocks of all muscle and
enzyme systems within the body. The functions of proteins
are nutritional, biological, and structural. At the structural
level, proteins include collagen, actin, myosin, and other mus-
culoskeletal and cytoskeletal proteins that participate in the
structure and strength of the body and its individual cells.
At the molecular level, proteins participate in almost every
proc ess within cells, including establishing cell shape, providing
mechanical strength, locomotion, chromosome separation
in mitosis and meiosis, and facilitating intracellular transport
of nutrients.
Enzymes, which are proteins, are vital for metabolism
and catalyze thousands of biochemical reactions in the body.
In addition, cell signaling, adhesion, and cycling, as well as
immune responses, all depend on proteins. Some hormones,
growth factors, and blood clotting factors are mostly pro-
teins and play a dynamic role in the body. We cannot syn-
thesize all the amino acids we need but must obtain these
essential amino acids from the proteins in the foods we
ingest. Complete proteins (those that contain all of the
essential amino acids in appropriate ratios) are digested
into the peptides and amino acids that are essential for
metabolism.
From the nutritional standpoint, nine amino acids are
considered essential because they cannot be synthesized in
our bodies and must, as a consequence, be consumed as part
of the diet. The essential amino acid distribution in various
foods varies dramatically. Food designers must consider the
amino acids present in assessing nutrition. For example,
sports recovery products should be rich in the branched
chain amino acids (BCAAs) such as valine, leucine, and iso-
leucine, which are most prevalent in whey proteins. These
amino acids cannot be made by the body, so they must be
consumed from food or supplements. Unlike other amino
acids, which are broken down in the liver, BCAAs are metabo-
lized in the muscles, where they produce energy or construct
new protein—that is, muscle.
The Protein Digestibility Corrected Amino Acid Score
(PDCAAS) is the preferred analytical method for evaluating
the protein quality of a food based on the amino acid require-
ments of humans and their ability to digest it. The PDCAAS
rating is universally recognized by the FAO/World Health
Organization (WHO), as well as by the U.S. Food and Drug
Administration (FDA), the USDA, and the National Academy
c16.indd 327 1/14/2016 12:50:46 PM
328 • CULINOLOGY®
of Sciences, as accurately measuring the correct relative
nutritional value of animal and vegetable sources of protein
in the diet.17
PDCAAS is based on human amino acid requirements,
which makes it more appropriate for humans than a method
based on the amino acid needs of animals. It is calculated
using the following formula, wherein the limiting amino acid
is the essential amino acid present in the least amount rela-
tive to the requirement for that amino acid:
milligrams of limiting amino acid in 1 gram of test proteinn
fecal true digestibility percentage
milligrams of same ammino acid in 1 gram of known reference protein
The fecal true digestibility percentage is the measurable
amount of the test amino acid found in the feces after the test
subject has finished digesting the test protein. The higher the
amount found in the feces, the less digestible the original pro-
tein source and the smaller the amount of the amino acid
that was absorbed by the body.
However, the PDCAAS does have limitations. For example,
it takes no account of where the proteins are digested. Any
amino acid that moves beyond the terminal ileum (small
intestine) is not likely to be absorbed for use in protein syn-
thesis. If it is then used by gut microflora, it will not be present
in the feces and will only appear to have been digested.
Similarly, any amino acid lost to antinutrients (digestion
inhibitors of many kinds) won’t show up in the feces either,
and will also be assumed to have been digested. The PDCAAS
method might also be considered incomplete because human
diets, except in times of famine, almost never contain only
one kind of protein.
However, calculating the PDCAAS of a total diet solely
based on each of its individual constituents is impossible.
That is because one food may provide an abundance of an
amino acid that the other is missing, which means the
PDCAAS of the total diet is higher than any one of the con-
stituents. For example, grain protein has a PDCAAS of 0.4 to
0.5, limited by lysine. On the other hand, it contains more
than enough methionine. White bean protein (and many
other pulses) has a PDCAAS of 0.6 to 0.7, limited by methio-
nine, but contains more than enough lysine. When both are
eaten in roughly equal quantities in a diet, the PDCAAS of the
combined constituent is 1.0 because each constituent’s pro-
tein is complemented by the other.18
Lipids
While they provide highly concentrated energy (9 kcal/g),
fats and oils have both positive and negative health effects.
Trans fats and saturated fats are considered negative because
they are associated with coronary heart disease, Alzheimer’s
disease, certain cancers, diabetes, and liver dysfunction.
Conversely, monounsaturated fats, such as those in olive oil,
are considered healthy, as are the omega‐3 oils found in fatty
fish, which have been recognized to provide clear health
benefits.
Although good and bad fats have been getting a lot of
attention for some time now, consumers are still asking for
confirmation of which is which. Although all fats are high in
calories, they are not all equal nutritionally. Depending on
their structure, the fats in foods can be classified as satu-
rated, monounsaturated, or polyunsaturated fats. Most
foods contain a combination of fats, usually with one kind
predominating.
The USDA recommends that fats should account for
approximately 30 percent of daily calories, with saturated fats
accounting for no more than 10 percent of total fat intake.
Yet, the real emphasis should remain on the type of fat.
Vegetable oils, avocados, nuts, and seeds may derive 85 to 100
percent of their calories from fat, but they are considered
good fats because they are primarily composed of unsatu-
rated fats.
Monounsaturated fats come primarily from plants, such
as nuts, avocados, and olives, as well as the oils made from
them, such as peanut, sesame, corn, canola, and olive oils.
They tend to lower LDL cholesterol (the bad cholesterol)
while increasing HDL cholesterol (the good cholesterol).
Polyunsaturated fats are also found mostly in plant sources,
such as grain products, soybeans, sunflowers, safflowers, cot-
tonseed, and flaxseed. These fats also tend to lower blood
cholesterol levels. A special category of healthy polyunsatu-
rated fats is known as omega‐3 fatty acids. Omega 3s are found
in oily fish like mackerel, salmon, trout, sardines, herring, and
tuna as well as in flaxseed, and are shown to help control cho-
lesterol and reduce blood pressure.19
Saturated fats and trans fats are known as bad fats because
they are associated with higher LDL cholesterol levels in the
blood and may cause an increased chance of clogged arteries.
Clogged arteries make it harder for blood to get to and from
the heart and can lead to high blood pressure, heart attack, or
stroke. Saturated fats are found mainly in animal products
like meat, poultry, lard, butter, regular milk, and egg yolk; cho-
lesterol is found exclusively in animal fats. Trans fats are
found mostly in man‐made solid fats made from vegetable
oils via hydrogenation, like shortenings, partially hydrogen-
ated vegetable oils, and stick margarine. Consumption of
trans fats is primarily from eating foods made with them—
potato chips, crackers, pastries, snack cakes, cookies, French
fries, and so on. The bottom line is that a diet high in satu-
rated fats, trans fats, and cholesterol increases the risk of
heart disease.
For more on lipids, see Chapter 9.
Cholesterol
Cholesterol is found only in animal fats, even unsaturated
ones like fish oils, because cholesterol is an essential com-
ponent of all animal cell membranes. In general, the higher
the blood cholesterol, the more likely that heart disease
will occur. As cholesterol is soluble in fat and blood is
mostly water, cholesterol requires assistance from proteins
to become soluble in blood. The two most influential proteins
c16.indd 328 1/14/2016 12:50:46 PM
CHAPTER 16 • Developing Nutritious Food Products • 329
are high‐density lipoproteins (HDL) and low‐density lipopro-
teins (LDL). HDL could also stand for heart disease lowering
because it helps remove cholesterol from the blood, while
LDL is commonly referred to as “bad cholesterol” because it
carries cholesterol that can be incorporated into the plaque
that builds up in the arteries and causes heart disease.
Therefore, an increased level of HDL and a decreased level of
LDL in the blood is most desirable. One of the worst effects of
consuming trans fats is that it does just the opposite: It raises
LDL and lowers HDL.
Cholesterol is also an essential structural component
of all mammalian cell membranes, of which it makes up
approximately 20 percent. It is required to establish proper
membrane permeability and fluidity and is thus manufac-
tured by every cell. Within the cell membrane, cholesterol
also functions in intracellular transport, cell signaling, and
nerve conduction. Cholesterol is essential for all animal life.
For a man of about 150 pounds, typical total body‐
cholesterol synthesis is about 1 g (1000 mg) per day, and total
body content is about 35 g (a little over an ounce), primarily
located within the membranes of all the cells of the body.
Typical daily dietary intake of additional cholesterol in the
United States is 200 to 300 mg. All foods containing animal
fat contain cholesterol to varying extents. However, most
ingested cholesterol is poorly absorbed, and the body com-
pensates for any absorption of additional cholesterol by
reducing internal cholesterol synthesis. For these reasons,
cholesterol intake in food has little effect on total body
cholesterol content or concentrations of cholesterol in the
blood. It is the intake of excessive amounts of saturated fats
and trans fats that has the greatest effect on increasing blood
cholesterol levels.
Omega‐3 and Omega‐6 Fatty Acids
Linoleic acid and alpha‐linolenic acid, known as omega‐6 and
omega‐3 fatty acids, respectively, are considered essential fatty
acids because the body cannot make them, meaning people
must get them from food or supplements. Both are necessary
for human health and are polyunsaturated fats that are stored
in the fatty tissues and can be used as a source of energy. They
also do a variety of important jobs in the body, from bolstering
immune system functions to helping form cell membranes
and producing hormone‐like compounds. Omega‐6 fatty
acids can be found in sunflower, safflower, corn, and soybean
oils, while omega‐3 fatty acids can be found in oily fish, wal-
nuts, and dark, leafy green vegetables as well as flaxseed, soy-
bean, and corn oils. The ratio of omega‐6 to omega‐3 fatty
acids in the diet has proved significant because the functions
of one are adversely modified by high amounts of the other.20
Current data indicates that it is best to consume a 4 (or less) to
1 ratio of omega‐6 to omega‐3 fatty acids. Unfortunately, the
average U.S. diet is closer to 15 or 20 to 1 due to the large
amounts of wheat, corn, and rice consumed, as well as poly-
unsaturated oils, margarines, and foods containing them.
While playing a crucial role in brain function as well as
normal growth and development, omega‐3s have also been
shown to reduce the risk of heart disease and can reduce
inflammation with respect to cancer and arthritis. Two
important omega‐3s are eicosapentaenoic acid (EPA) and
docosahexaenoic acid (DHA), which naturally occur in fatty
fish such as salmon, tuna, sardines, anchovies, striped bass,
catfish, herring, mackerel, trout, and halibut. The American
Heart Association (AHA) suggests consuming one or more of
these fish at least twice a week to help reduce the risk of heart
disease, heart attack, and stroke.21
Because the majority of Americans do not consume fish at
adequate levels, alternative approaches are needed to deliver
sufficient omega‐3 lipids. Formulated foods present signifi-
cant technical barriers due to the extremely unstable nature
of omega 3s and their propensity to smell and taste extremely
fishy. Like all polyunsaturated oils, they are extremely vulner-
able to oxidative breakdown. One successful approach is to
add oils rich in omega‐3s to poultry feed, with the result that
the chickens deposit omega‐3 fatty acids in the egg yolk.
Another approach is microencapsulation. There are many excel-
lent sources of microencapsulated omega‐3 fatty acids. Unfor-
tunately, they tend to be fairly expensive and the encapsulated
materials generally deliver less than 50 percent oil by weight
due to fillers and stabilizers in the encapsulation material.
Other unsaturated fatty acids in the diet, such as oleic,
linoleic, and linolenic, are beneficial by reducing blood triglyc-
eride levels, reducing platelet aggregation, and providing anti‐
arrhythmic effects. The polyunsaturated omega‐3, omega‐6,
Polyunsaturated Fatty Acids
(PUFA)
Omega-3
(Alpha-linolenic acid)
Omega-6
(Linoleic acid)
Eicosapentaenoic acid
(EPA)
Docosahexaenoic acid
(DHA)
Arachidonic acid
FIGURE 16.4 Breakdown of essential fatty acids.
c16.indd 329 1/14/2016 12:50:46 PM
330 • CULINOLOGY®
and omega‐9 fatty acids, as well as the conjugated fatty
acids (polyunsaturated fatty acids in which at least one
pair of double bonds is separated by only one single bond),
are also essential for infants (studies report increased vis-
ual activity, cognitive development, immune function, and
motor functions).22
Conjugated linoleic acid (CLA), found in highest levels in
grass‐fed beef and lamb and also in eggs (and, interestingly,
in white button mushrooms), is shown to have a remarkable
number of health benefits, including anti‐cancer and anti‐
inflammatory properties, as well as being a useful tool in
weight management.23 It does not cause weight loss directly,
but over time it gradually replaces body fat with lean muscle
mass.24 This is a healthy effect, as a high degree of fat mass is
related to all causes of mortality, and lean body mass burns
more calories than fat mass, which may help increase resting
metabolic rates. In July 2008, CLA received a no‐objection
letter from the FDA on its request for Generally Recognized
as Safe (GRAS) status for certain food categories, including
fluid milk, yogurt, meal replacement shakes, nutritional
bars, fruit juices, and soy milk.25 With GRAS status, food
companies may now add CLA to products in these food
categories.
FIGURE 16.5 Health effects and sources of common fatty acids.
Fatty Acid Group Health Effects Sources
SHORT CHAIN
Acetic, propionic, butyric,
all from fermentation in gut.
Only butyric in natural dietary fats.
SFAs are produced in colon and
are associated with lower cancer risk,
satiety, and reduced weight gain.
Butyric is in butterfat, all
SCFAs are bound in the low
calorie fat Salatrim.
MEDIUM CHAIN
Caproic, capric, and caprylic
MCTs are rapidly absorbed and
burned as energy source. Used in
enteral nutrition.
Found in coconut oil.
Also in restructured lipids for
nutritional foods.
LONG CHAIN SATURATED
Lauric, myristic, palmitic, stearic
Palmitic and stearic are
hypercholesterolemic and thus
associated with cardiovasucular risk.
Lauric— coconut oil
Myristic—palm kernel oil
Palmitic—palm oil and
animal fat
Stearic—animal fat, some
plant oils, hydrogenated oils
MONOUNSATURATED
Oleic (cis)
Elagic (trans)
Oleic less hypercholesterolemic.
Associated with healthy Mediteranian diet.
Elagic is hypercholesterolemic and
inflammatory.
Oleic is from olive oil, canola
oil, and many other plant
lipids.
Elagic is formed in
hydrogenated oils.
POLYUNSATURATED
Gamma‐linoleic
Essential fatty acid required prostaglandin
synthesis. Important for inflammatory
response, immunity.
Needs to be balanced with omega‐3 fatty
acids.
Found in most plant lipids
and in fish oils.
Major n‐6 polyunsatured
fatty acid in the diet.
Omega 3s
Alpha linoleic
DHA, EPA
Omega‐3 fatty acids are associated with
reduced cardiovascular risk, reduced
Alzheimer’s risk.
Alpha‐linoleic is found in
flax oil.
EPA and DHA are found in
algal oil and fish oil.
Source: Darryl Holliday.
Oleic acid (the predominant fatty acid in olive oil) has a
lower melting point than trans fats and the saturated fatty
acids. As a result, one of the benefits of oleic acid may be that
when it is incorporated into cell membranes, they are more
fluid than those containing the higher-melting-point fats,
allowing for easier delivery of nutrients and removal of toxins
as well as aiding in cell communication.
The polyunsaturated fatty acids, particularly the highly
unsaturated fish oils, are prone to oxidation and rancidity
and thus present flavor and stability problems in foods. The
polyunsaturated fatty acids are also vulnerable to damage
during high‐heat applications such as frying. Stabilization
with antioxidants enhances the shelf life of these products.
When formulating foods rich in polyunsaturated fats, it is
necessary to provide protection either with natural antioxi-
dants, such as tocopherols (forms of vitamin E), or syn-
thetic antioxidants, such as TBHQ (tert‐butylhydroquinone),
BHA (butylated hydroxyanisole), and BHT (butylated hy-
droxytoluene). Plant extracts, such as rosemary extract,
are also used in certain applications, but they are generally
less effective than the stronger and less expensive syn-
thetic antioxidants, and they provide an often undesirable
herbal flavor.
c16.indd 330 1/14/2016 12:50:47 PM
CHAPTER 16 • Developing Nutritious Food Products • 331
Carbohydrates
Carbohydrates represent a broad variety of ingredients that
can be present in or applied to foods. They range in size from
3‐carbon glycerine and 5‐ and 6‐carbon simple sugars to very
large and complex starches and fiber. Carbohydrates repre-
sent the greatest percentage of nutrients (and calories) in our
diets and, therefore, contribute major physical as well as bio-
chemical functionality.
Sugars
Because the major function of sugars in foods is to provide
sweetness, let us look at the relative sweetness of each com-
pared to sucrose (table sugar), which is assigned a value of 100.
It is important to note that high‐fructose corn syrup
(HFCS) has virtually taken over as the sweetener of choice in
most food and beverages manufactured in the United States
due to its level of sweetness (approaching sucrose) and lower
production cost. Although all of the HFCS sweeteners are
fully caloric and deliver 4 kcal/g, evidence suggests that fruc-
tose is not digested the same as sucrose and in fact may be
responsible for several harmful health effects (including
increasing the rate of obesity, increasing insulin resistance
and other diabetes‐related complications, and contributing
to fibrosis of the liver).26 To date, no incontrovertible data
shows a direct cause‐and‐effect relationship. That said, food
and beverage manufacturers are replacing HFCS with sucrose
in many products to stave off a potential consumer boycott of
FIGURE 16.7 Sweetness value of sugars.
Sugar Sweetness
Sucrose 100
Glucose 70
Fructose 120
Maltose 45
Lactose 40
Corn syrup (glucose, maltose) 30–50
High-fructose corn syrup 80–90
Invert sugar 95
Source: Darryl Holliday.
saturated mono trans n-6 n-3
0 20 40 60 80 100
Beef Fat
Chicken
Lard
Butter Oil
Hydrogenated Coconut Oil
Filler Fat
Olive Oil
Peanut Oil
Canola Oil
Corn Oil
Soy Oil
High Oleic Sunflower Oil
Cocoa Butter
Palm Kernel Oil
Flax Oil
Menhaden Oil
Salmon Oil
Sardine Oil
FIGURE 16.6 Typical composition of fats and oils.
HFCS‐containing foods. While this trend is slowly increasing,
the industry has not made a complete change due to the
price and functional attributes of HFCS.
With the emphasis on lower‐calorie foods, it is important
to consider the use of high‐intensity non‐nutritive sweeten-
ers. Application of these intense sweeteners varies because
of their differences in relative sweetness, heat and pH stabil-
ity, taste and aftertaste, and interactions with other flavors.
Two products sold commercially in the United States, stevio-
side (Truvia®, PureVia®, others) and mogroside from luo han
guo (Monkfruit in the Raw™), are considered natural.
Source: Darryl Holliday.
c16.indd 331 1/14/2016 12:50:47 PM
332 • CULINOLOGY®
While providing sweetness similar to sucrose, the intense
sweeteners present problems in developing functional foods
because of their use at very low levels. They also do not add
bulk, contribute to texture, or control water activity. As an
alternative, sugar alcohols (polyols) can be used for sweet-
ness and to provide bulk in low‐ and no‐sugar applications.
The sugar alcohols are not well digested, so they generally
add some calories, but less than sugars do. In addition, all of
the polyols are beneficial for use in diabetic foods because
they do not influence blood glucose levels. Generally, they are
not used by bacteria in the mouth, so they are non‐cariogenic
when used in chewing gums and confections. However, they
can be fermented by colonic bacteria, leading to gastric dis-
comfort when taken in large quantities. Listed below are the
relative sweetness and caloric values of the sugar alcohols.
Artificial Sweeteners
Artificial sweeteners are used instead of sucrose (table sugar) to
sweeten foods and beverages without adding calories. Because
artificial sweeteners are many times sweeter than table sugar,
smaller amounts are needed to create the same level of sweet-
ness. In the United States, artificial sweeteners are regulated
by the FDA. Approved artificial sweeteners include saccharin
(Sugar Twin, Sweet ‘n’ Low, others), aspartame (NutraSweet,
Equal, others), acesulfame‐potassium (Sunette, Sweet One),
sucralose (Splenda), neotame, and tagatose.
FIGURE 16.8 Sweetness value of high‐intensity
zero‐calorie sweeteners.
Sweetener Sweetness Application Note
Mogroside 250 Plant source
Cylamate 3000 Banned in United States
Acesulfame‐K 20,000 Heat stable
Aspartame 18,000 Not stable for cooking
Saccharin 30,000 Heat stable
Neotame 800,000 Heat stable
Stevioside 30,000 Plant source
Sucralose 60,000 Heat stable
Source: Darryl Holliday.
FIGURE 16.9 Sweetness value of sugar alcohols.
Sugar
Alcohol
Sweetness (Compared
to Sucrose)
Caloric Value
(Kcal/g)
Erythritol 70 0.2
Xylitol 100 2.4
Mannitol 70 1.6
Sorbitol 60 3.0
Maltitol 90 3.0
Lactitol 40 3.0
Source: Darryl Holliday.
FIGURE 16.10 Cariogenic attributes of
sweeteners.
Sweetener Cariogenic “Sugar‐free” Label
Natural sugars
Sucrose Yes No
Glucose Yes No
Fructose Yes No
Lactose Yes No
Sugar substitutes
Sugar alcohols/polyols
Xylitol No Yes
Sorbitol No Yes
Mannitol No Yes
Maltitol No Yes
Artificial sweeteners
Aspartame* No Yes
Saccharin No Yes
Sucralose No Yes
Acesulfame potassium No Yes
*Aspartame is technically a nutritive sweetener, but because of its
intense sweetness it is used in such small amounts that its nutritive
value is negligible.
Adapted from K. A. Ly, P. Milgrom, and M. Rothen, “Xylitol,
Sweeteners, and Dental Caries,” Pediatric Dentistry 28, no. 2 (2006):
154–164.
Dietary Fiber
Dietary fiber comes from portions of edible plants that are
not digested by humans. Dietary fiber includes a broad range
of polysaccharides, including cellulose, as well as many other
plant components, such as dextrins, inulin, lignin, chitins,
pectins, beta‐glucans, oligosaccharides, and several types of
non‐digestible starch. What they share is that they cannot be
broken down by human enzymes and, therefore, provide no
calories. Thus, large quantities of high‐fiber foods can be
eaten to provide fullness without increasing caloric content.
In addition, they add bulk to help solid waste move through
the digestive tract.
This mixture of indigestible materials can be divided into
soluble (fermentable) fiber and insoluble (non‐fermentable)
fiber, both of which are present in plant foods. Insoluble fiber
passively attracts water, increasing bulk, which results in
reduced transit time of solid waste through the gastrointesti-
nal tract.
Soluble fiber (considered a prebiotic), absorbs water to
become a gelatinous, viscous substance that is fermented by
bacteria (the probiotics) in the digestive tract, yielding vari-
ous compounds that produce significant health benefits:
c16.indd 332 1/14/2016 12:50:47 PM
CHAPTER 16 • Developing Nutritious Food Products • 333
• Helps regulate blood glucose and lipid levels.
• Helps lower total and LDL “bad” cholesterol levels.
• Delays absorption of glucose after a meal to help reduce
spiking of blood sugar levels.
• Balances intestinal pH and protects against infectious
diseases by out‐competing pathogens for intestinal real
estate.
• Helps regulate immune response.
• Improves absorption of minerals.27–31
Known side effects from ingesting large amounts of solu-
ble fiber are the formation of fermentation products such
as gas and laxative compounds. In most cases, effects are
reduced or subside as the body gets accustomed to the change
in diet.
Many foods contain both soluble and insoluble fiber. Fruit
skins tend to contain insoluble fiber, whereas the flesh and
pulp contain more soluble fiber. Whole grains, bran, flaxseed,
and vegetables are rich sources of insoluble fiber. Peas, beans,
oats, apples, and carrots are good sources of soluble fiber.
The health benefits of both soluble and insoluble fiber
make them useful for development of healthier foods. For
instance, adding fiber produces caloric reduction by simple
dilution. Fibers such as prune paste and inulin can act as fat
mimetics, providing fatty texture with few or zero calories.
Also, certain fibers are shown to lower cholesterol. For exam-
ple, the marketing of whole‐grain cereals based especially on
oat fiber has been successful, as beta‐glucans (found in oat
fiber) are proven to control serum cholesterol. Other fiber‐
containing foods provide similar opportunities. However, for-
mulating foods with high fiber can offer technical challenges
because both soluble and insoluble fiber bind many times
their weight in water. This can result in negative textures, such
as cakey cookies and poor bread loaf volume, and reduced
shelf life due to higher water activity (a
w
), leading to increased
mold growth. Formulating these ingredients into products
such as cookies, bread, and pasta requires formulation adjust-
ments to deal with the excess of water binding in these foods.
These adjustments can include everything from increased
baking times to substitution with resistant starches.
Polydextrose (Litesse®, Sta‐Lite®, others) is a synthetically
produced glucose polymer (89% glucose, 10% sorbitol, 1% citric
acid) that provides 1 kcal/g. It is classified as a soluble fiber
that is only partially metabolized by gut microbes. Its taste is
non‐sweet and innocuous, so it doesn’t add any flavor to the
foods it is added to, and it is highly soluble and clear in solu-
tion. It is commonly used to increase fiber content, replace
sugar, reduce fat content, and reduce calories in an assortment
of foods and beverages. It can also be used as a humectant
(moisture‐holding agent), stabilizer, bulking agent, and thick-
ening agent. It is useful in both dietetic and diabetic applica-
tions. Polydextrose allows the product developer to add fiber
and lower calories from sugar and fat without compromising
taste or texture. In the United States, the FDA has approved
the use of polydextrose in all foods with the exception of meat,
poultry, baby food, and infant formula.
Resistant Starches
Starch (which is normally digestible) can act like dietary fiber
when it is in one of four forms:
RS1—Physically resistant starch, which cannot be digested
by mammalian enzymes. It is typically found in raw, unproc-
essed grains and legumes.
RS2—Natural granular starch found in raw potato, green
banana, and high‐amylose corn.
RS3—Starch that has been gelatinized (cooked) and cooled,
causing recrystallization (retrogradation). Found in stale
bread and cooked and chilled potatoes.
RS4—Starches that have been chemically modified to pro-
duce enzyme resistance.
Resistant starches are a powerful new tool for dietary food
developers because they provide only 2 to 3 kcal/g, compared
to 4 kcal/g for fully digestible carbohydrates. This caloric
reduction is useful in the formulation of reduced‐ calorie
foods. The slower digestion of the starch also prevents a spike
in blood glucose after eating. This leads to an increased feel-
ing of fullness as well as a consistent energy release instead of
a quick energy boost and sudden crash. Because the release
of glucose during the digestion of resistant starch is slower
than in gelatinized (cooked) starch, the glycemic index is
much lower. This is especially important in the development
of foods for diabetics.
Substantial research on the health benefits of resistant
starch includes more than 200 published, peer‐reviewed
studies that indicate benefits in intestinal and colonic health
as well as metabolically important benefits in glycemic man-
agement and calorie reduction.
Just a few examples:
• A recently published clinical trial by Dr. Joanne Slavin
and her colleagues at the University of Minnesota dem-
onstrated that eating food made with resistant starch
from high‐amylose corn had more of an impact on satiety
than other types of fibers (corn bran, barley beta‐glucan,
and polydextrose). This study suggests that all fibers do
not impact satiety equally, so the type of fiber must be
carefully considered in new product development.32
• Of several studies published by Dr. Denise Robertson and
her colleagues at the Oxford Lipid Metabolism Group and
the University of Surrey (UK), the most recent found that
dietary consumption of resistant starch resulted in 10 per-
cent fewer calories being consumed in the following
24 hours. Also, calorie intakes were significantly lower at
the ad libitum (eat‐all‐you‐want) test meal.33
• Dr. Anne Nilsson and her colleagues at Lund University
(Denmark) found that when Hi‐maize resistant starch
and barley kernels (another natural source of resistant
starch) were consumed in the evening meal, the results
included improved glucose tolerance, lowered inflamma-
tory biomarkers, and increased satiety after a standard-
ized, high‐glycemic breakfast the following morning.34
c16.indd 333 1/14/2016 12:50:47 PM
334 • CULINOLOGY®
• Numerous studies demonstrate that RS2 and RS3 resist-
ant starches from high‐amylose corn promote intestinal
and colonic health through their fermentation and
action as prebiotic fibers. They encourage the growth of
health‐promoting bacteria, reduce pH, and increase the
production of butyrate via fermentation. Buytrate, a
short‐chain fatty acid, is important for colonic health
and is shown to have health‐promoting properties.35,36
Water
Water, which constitutes about 60 percent of the human body,
is a critical, often overlooked multifunctional component of our
bodies and diet. Water plays a vital role in the control of weight,
muscle tone, hypertension, cholesterol, bloating, constipa-
tion, ulcers, low energy levels, and stomach pain. It regulates
body temperature, removes toxins and wastes, cushions and
lubricates joints, decreases risk of kidney stones, and protects
tissues, organs, and the spinal cord from shock and damage. It
assists in the digestion and absorption of food and in trans-
porting oxygen and nutrients to the cells. All bodily enzymatic
and chemical reactions occur in the presence of water. Water
also transports hormones, nutrients, oxygen, and antibodies
through the bloodstream and lymphatic system. Additionally,
it contains zero calories, zero fat, and zero cholesterol, and is
very low in sodium. Plus, it can act as an appetite suppressant.
Studies show that a low consumption of water allows more fat
to be deposited instead of being metabolized. The greater the
amount of water consumed, the less fat is deposited in the
body. To put it simply, drinking a sufficient amount of water on
a daily basis is essential for good health.37,38,39
In our bodies, dehydration results when water output
exceeds water input. Symptoms become more severe as more
water is lost. Based on body weight, a 1 to 2 percent loss of
water induces thirst, fatigue, weakness, and vague discom-
fort. When 7 to 10 percent of body weight is lost, dizziness,
muscle spasms, delirium, and collapse can result. Average
urine output for adults is about 1.5 L a day, plus an additional
liter of water a day lost through breathing, sweating, and
bowel movements. Food accounts for approximately
20 percent of our total fluid intake. Consumption of 2 L (a
little over 8 cups) of water or other beverages daily generally
replaces the fluid lost. This idea is consistent with the popu-
lar 8 × 8 rule, which recommends eight 8‐ounce servings of
fluids per day, and is responsible for the phenomenal popu-
larity of bottled water (a $60 billion/year global industry).
Micronutrients: Vitamins
and Minerals
Although required in small quantities, micronutrients, in-
cluding vitamins and minerals, are essential to our health.
Minerals fall into two categories: major and trace, depending
on the quantities of each required daily. The major minerals
are calcium, phosphorous, potassium, sulfur, sodium, chlo-
rine, and magnesium. The trace minerals are iron, zinc, sele-
nium, manganese copper, iodine, molybdenum, chromium,
and fluorine. Proper balance of trace elements is critical
because too little can result in a deficiency, while too much
can result in toxicity. One way to ensure one’s diet encom-
passes all required micronutrients is to follow the recom-
mendations set forth in the Dietary Guidelines for Americans.
Vitamins are divided into fat‐soluble (A, D, E, and K) and
water‐soluble (B‐complex and C). Both vitamins and minerals
help regulate metabolic functions. They play key roles in
the body’s innumerable enzyme‐catalyzed reactions, where
they serve as co‐enzymes (vitamins) or co‐factors (minerals)—
without which the enzymes cannot function properly. Because
they play such vital roles in the body’s processes, many foods
are now enriched or fortified with vitamins and minerals. For
example, in the milling of wheat to make flour, several vitamins
and minerals are lost. Those specific nutrients are added back
in mandated amounts (2.9 mg thiamine, 1.8 mg riboflavin,
24 mg niacin, 0.7 mg folic acid, and 20 mg iron per pound of
flour) in order to produce enriched flour. Fortified foods are
products to which nutrients are added that were not originally
present, such as calcium to orange juice and B vitamins to
cereals. Because these foods are standard items in most peo-
ple’s diets, they are used as convenient delivery systems for
these essential nutrients to the general population.
FIGURE 16.11 The roles of vitamins and minerals in health.
Fat-Soluble Vitamins
Vitamin Functions Sources
Vitamin A Promotes vision, growth, and resistance to
bacterial infection and overall immune system
function while preventing dry skin and eyes
Liver, sweet potatoes, spinach, greens, carrots,
cantaloupe, apricots, broccoli, fortified milk, and
breakfast cereals
Vitamin D Increases absorption of calcium and
phosphorus and maintains optimal blood
calcium and calcification of bone
Fish oils, sardines, salmon, fortified milk, and
breakfast cereals
Vitamin E Antioxidant; prevents breakdown of vitamin A
and unsaturated fatty acids
Plant oils, cabbage, asparagus, sweet potatoes,
tomatoes, apples, avocados, mango, shrimp, nuts,
seeds, fortified breakfast cereals
Vitamin K Activates blood‐clotting factors and proteins
involved in bone metabolism
Green vegetables, liver, some plant oils
(continued)
c16.indd 334 1/14/2016 12:50:47 PM
CHAPTER 16 • Developing Nutritious Food Products • 335
Water-Soluble Vitamins
Vitamin Functions Sources
Biotin Coenzyme of glucose and fat synthesis Cheese, egg yolks, cauliflower, peanut butter, liver
Choline Neurotransmitter and phospholipid synthesis Widely distributed in foods and synthesized by
the body
Folate
(folic acid)
Coenzyme involved in DNA synthesis, among
many other functions
Green leafy vegetables, orange juice, organ meats,
sprouts, sunflower seeds
Niacin Coenzyme of energy metabolism, fat synthesis,
and fat breakdown
Mushrooms, bran, tuna, salmon, chicken, beef,
liver, peanuts, enriched grains
Pantothenic
acid
Coenzyme of energy metabolism, fat synthesis,
and fat breakdown
Mushrooms, liver, broccoli, eggs
Riboflavin Coenzyme of carbohydrate metabolism Milk, mushrooms, spinach, liver, enriched grains
Thiamin Coenzyme of carbohydrate metabolism and
involved in nerve function
Sunflower seeds, pork, whole and enriched grains,
dried beans, peas
Vitamin B
6
Coenzyme of protein metabolism and involved
in neurotransmitter and hemoglobin synthesis
Animal protein, spinach, broccoli, bananas, salmon,
sunflower seeds
Vitamin B
12
Coenzyme of folate metabolism and involved in
nerve function, among many other functions
Animal foods, organ meats, oysters, clams,
fortified ready‐to‐eat breakfast cereals
Vitamin C Connective tissue, hormone, and neurotransmitter
synthesis as well as possible antioxidant activity
Citrus, fruits, strawberries, broccoli, greens
Major Minerals
Mineral Functions Sources
Calcium Bone and tooth structure, blood clotting, aids in
nerve impulse transmission, muscle contractions,
and other cell functions
Dairy products, canned fish, leafy vegetables,
tofu, fortified orange juice, other foods
Chloride Major negative ion of extracellular fluid,
participates in acid production in stomach, aids
nerve impulse transmission and water balance
Table salt, some vegetables, processed foods
Magnesium Bone formation, and aids enzyme, nerve, and
heart function
Wheat bran, green vegetables, nuts, chocolate,
legumes
Phosphorus Major ion of intracellular fluid, bone and tooth
strength, part of various metabolic compounds,
acid/base balance
Dairy products, processed foods, fish, soft
drinks, bakery products, meats
Potassium Major positive ion of intracellular fluid, aids nerve
impulse transmission and water balance
Spinach, squash, bananas, orange juice, milk,
meat, legumes, whole grains
Sodium Major positive ion of extracellular fluid, aids nerve
impulse transmission and water balance
Table salt, processed foods, condiments,
sauces, soups, chips
Sulfur Part of vitamins and amino acids, aids in drug
detoxification and acid/base balance
Protein foods
Adapted from G. M. Wardlaw and A. M. Smith, Contemporary Nutrition, 7th ed. (New York: McGraw‐Hill, 2009).
One of the primary responsibilities in food product develop-
ment is to preserve the nutrients present (and those that might
be added) during all phases of processing and manufacture.
Therefore, knowledge of the stability of nutrients, particularly
vitamins, under different processing, packaging, and distri-
bution conditions is critical. For instance, vitamin A is highly
sensitive to acid, air, light, and heat, while vitamin C is stable in
acid but sensitive to alkalinity, air, light, and heat. Because of the
instability of certain nutrients under various conditions, and
their water solubility, cooking losses of some essential nutrients
can be greater than 75 percent. However, losses seldom exceed
25 percent in modern food processing operations.
FIGURE 16.11 (continued)
c16.indd 335 1/14/2016 12:50:47 PM
336 • CULINOLOGY®
Salt Substitutes
Salt, or sodium chloride (NaCl), is the most common source
of the mineral sodium in the diet. The body requires sodium
to function properly, but most Americans eat about 10 times
more than they actually need. The American Heart Asso-
ciation (AHA) currently recommends that healthy adults
should consume no more than 2400 milligrams each day—
that’s equivalent to 1 teaspoon of table salt. That said, the
AHA also states that “[r]educing daily intake to 1500 milli-
grams is desirable because it can lower blood pressure even
further.” A mere 15 percent of the salt in the average American
diet comes from natural sources in foods and water. The rest
comes from the salt added during food processing (about
67 percent) and at the stove and dining table (18 percent).
Sodium plays a key role in muscle function and in main-
taining the body’s fluid balance; however, it is easy to con-
sume too much. Over time, excess sodium can elevate blood
pressure levels, raising the risk of heart disease and stroke.
Reducing salt consumption helps reduce blood pressure and
therefore reduces risk. Food manufacturers have reacted to
sodium’s bad press by lowering its level in many food prod-
ucts. Campbell’s® and Progresso® lowered the sodium in their
soups in 2010, yet Campbell’s® did add some back to their
product line in 2012 due to decreased sales.40
The FDA and USDA state that an individual food claiming
to be “healthy” must not exceed 480 mg sodium per serving.
FIGURE 16.12 Vitamin and mineral stability.
Nutrient Acid Base Oxygen Light Heat Overage Needed for Processing
Vitamins
Vitamin A + √ + + + Up to 40%
Thaimin + √ + + √ Up to 60%
Riboflavin √ + √ + √ Up to 40%
Niacin √ √ √ √ √ Up to 10%
Vitamin B
6
+ + √ + √ Up to 40%
Vitamin B
12
+ + + + √ Up to 75%
Pantothenic acid + + √ √ + Up to 50%
Folic acid + + + + √ Up to 80%
Biotin + + √ √ √ Up to 60%
Vitamin C + + + √ + Up to 100%
Vitamin D + + + + + Up to 75%
Vitamin E √ + + + + Up to 40%
Vitamin K √ √ + + √ Up to 25%
Minerals √ √ √ √ √ Up to 3%
Amino acids √ √ √ √ + Up to 25%, average of 10–15%
Fatty acids √ √ + + + Up to 55%, average of 10–15%
+ = sensitive; √ = not sensitive
Source: Darryl Holliday.
Meal‐type products must not exceed 600 mg sodium per
labeled serving size.
While most sodium is consumed in the form of sodium
chloride, a wide variety of common food ingredients and con-
diments also contain sodium, such as soy sauce, ketchup,
mustard, salted butter, and monosodium glutamate (MSG).
A majority of salt substitutes use potassium chloride (KCl).
The problem with this is that while KCl does have a salty
taste, it also has an unpleasant bitter or metallic aftertaste, so
it is commonly blended with NaCl to reduce bitterness and
provide sodium reduction rather than total replacement.
Other sodium‐containing ingredients, such as sodium
bicarbonate (baking soda) can also be substituted for with
FIGURE 16.13 Sodium labeling.
• Sodium‐free: less than 5 milligrams of sodium per
serving
• Very low‐sodium: 35 milligrams or less per serving
• Low‐sodium: 140 milligrams or less per serving
• Reduced sodium: usual sodium level is reduced by
25 percent
• Unsalted, no salt added, or without added salt: made
without the salt that’s normally used, but still contains
the sodium that’s a natural part of the food itself
Source: Darryl Holliday.
c16.indd 336 1/14/2016 12:50:48 PM
CHAPTER 16 • Developing Nutritious Food Products • 337
potassium‐based ingredients, such as potassium bicarbonate.
However, salt substitutes containing potassium are not safe
to consume for everyone. For example, people with chronic
kidney disease have difficulty filtering out excessive amounts
of potassium.
Other salt‐reduction strategies include substituting high
mineral–containing sea salts, which have less sodium than
table salt due to the presence of a multitude of other naturally
occurring mineral salts. Of course, this is as variable as the
ocean, so one cannot assume that every sea salt has the same
amount of sodium (or potassium); it’s important to check the
label. In addition, substituting an equal volume of a larger
crystal-size salt (such as kosher salt) for finer table salt can
result in less salt being used, as the coarser salt contains less
sodium by volume.
FIGURE 16.15 Salt equivalents.
Iodized
Table Salt Kosher Salt Sea Salt
Pickling
Salt
¼ tsp ¼ tsp ¼ tsp ¼ tsp
1 tsp 1¼ tsp 1 tsp 1 tsp
1 tbsp (3 tsp) 4 tsp 3½ tsp 1 tbsp
¼ cup ¼ cup + 1 tbsp ¼ cup + 1 tsp ¼ cup
1 cup 1¼ cups 1 cup + 1½ tbsp 1 cup + 2 tsp
Source: Morton Salt Company, www.mortonsalt.com.
Health Issues
and Trends
Nutrition for Special Groups
Diets are as diverse as people. Given their personal and reli-
gious beliefs, ethnicity, activity levels, health status, and age,
many people have dietary restrictions and preferences.
Kosher and Halal
Special dietary considerations must be addressed for various
groups when formulating food products for those mar-
kets. Two major religions with strict dietary restrictions are
Judaism and Islam. While the two religions have some similar
practices, there are key differences. Kosher laws, followed by
Jews, and halal laws, observed by Muslims, each describe
what is fit and proper or lawful for members of these groups
to eat.
Kosher
Deuteronomy, in the Torah, lists clean and unclean animals
and specifies the characteristics of kosher animals. In strict
Jewish observance, for example, it is permissible to eat any
animal that has a split hoof and that chews the cud (food of a
ruminant regurgitated to be chewed again). Cattle, sheep,
goats, deer, and bison are all kosher meats; however, the pig is
considered unclean because although it has a split hoof, it
does not chew the cud. To be kosher, animals must be slaugh-
tered according to ritual requirements and must not be
injured or ill in any way. In handling, kosher meat must be kept
separate from non‐kosher. Some other considerations regard-
ing meat are: Blood may not be consumed, hence the kosher
requirement of soaking meat in salt and water after slaughter,
along with a deveining step. The sciatic nerve is also to be
avoided. That means that in the United States (except in rare
instances in Jewish neighborhoods where religiously trained
butchers are able to recognize and remove the nerve success-
fully), only meat from the forequarter of the animal is consid-
ered kosher. With poultry, on the other hand, the entire animal
is considered kosher. With respect to sea creatures, kosher
laws permit eating any animal that has fins and removable
scales; however, those that do not possess these traits are
unclean and cannot be eaten (shellfish, mollusks, and so on).
Under kosher laws, meat and dairy cannot be eaten
together. For example, cheeseburgers may not be eaten, hot
dogs and milkshakes cannot be eaten together, and pepper-
oni pizza cannot be eaten if mozzarella is present. Eating
meat and fish together is also prohibited in the same dish,
but they can be eaten at the same meal.
These meat and dairy restrictions extend beyond foods to
the pots and pans, plates and utensils, dishwashers, and tow-
els on which they are dried. Many kosher kitchens have at
least two sets of pots, pans, and dishes, one for meat and one
for dairy.
This is equally important for the food manufacturer who
intends to produce kosher food. All manufacturing equipment
FIGURE 16.14 Sodium contents.
Sodium Source Portion Sodium Content
Curing and pickling salt 1 teaspoon 2360 mg
Iodized table salt 1 teaspoon 2325 mg
Sea salt, fine 1 teaspoon 2240 mg
Sea salt, coarse 1 teaspoon 1872 mg
Kosher salt 1 teaspoon 1120 mg
Baking soda 1 teaspoon 1000 mg
Source: Darryl Holliday.
FIGURE 16.16 Sodium reduction levels.
Food Product
Sodium (mg)
in Traditional
Version
Sodium (mg)
in Low‐Sodium
Version
Progresso Chicken
Noodle Soup
950 470
Campbell’s Chunky
Chicken Noodle Soup
870 480
Kikkoman Soy Sauce 920 575
Poore Brothers Kettle
Chips
180 90
Nabisco Ritz Crackers 135 35
Source: Darryl Holliday.
c16.indd 337 1/14/2016 12:50:48 PM
http://www.mortonsalt.com
338 • CULINOLOGY®
must be blessed by a rabbi before use, and if used for both
kosher and non‐kosher production, must be extensively
cleaned—“kosherized”—according to kosher requirements.
Likewise, kosher ingredients must be stored separately and
are not allowed to commingle with non‐kosher ingredients.
In order to get approval for a food label to carry the certified
kosher logo from one of the kosher certifying agencies, the
company must first go through a lengthy, detailed, and costly
application process and review of its entire manufacturing
process, from supply chain through distribution, before the
agency will give the go‐ahead.
With a total world population of Jews at less than 15 mil-
lion (around 6 million in the United States), it would seem
that there would be a somewhat limited consumer base to
support a kosher food line or business. However, the quality
reputation that kosher food and manufacturing have devel-
oped over the years has broadened the market for kosher
foods considerably, such that even non‐Jews consider kosher
foods to be better in many cases than their non‐kosher equiv-
alents. A former advertisement for a popular kosher hot dog
brand captured this sentiment perfectly when it stated, “We
answer to a higher authority.”
Passover is one of the most important Jewish holidays.
This is the time of year when food and beverage manufactur-
ers who replaced sugar with high‐fructose corn syrup must
go back to using sugar if they wish to label their products
Kosher for Passover. This is because corn (and corn‐derived
products) is one of the prohibited grains that must not be
consumed during this holiday, which commemorates the
Exodus: the escape of the Jews out of Egyptian slavery.
Halal
Muslims, according to the Koran, are allowed to eat what is
“good”—that is, what is “pure, clean, wholesome, nourishing,
and pleasing to the taste.” In general, everything is allowed
(halal) except what has been specifically forbidden. This is in
the interest of health and cleanliness, and in obedience to
God. In the Koran, the following foods and drinks are strictly
prohibited by God:
• dead meat (i.e., the carcass of an already‐dead animal)
• blood
• flesh of swine (pork)
• intoxicating drinks (including wine, beer, and spirits)
• meat of an animal that has been sacrificed to idols
• meat of an animal that died from strangulation or blunt force
• meat from which wild animals have already eaten
The Koran directs Muslims to slaughter their livestock by
slitting the animal’s throat in a swift and merciful manner,
reciting God’s name with the words, “In the name of God,
God is Most Great.” The animal is then bled completely before
consumption. Meat prepared in this manner is considered to
be “halal.” Some Muslims will abstain from eating meat if they
are uncertain of how it was slaughtered. They also place
importance on the animal having been bled properly, as oth-
erwise it would not be considered healthy to eat. Some
Muslims living in predominantly Christian countries hold the
opinion that one may eat commercial meat (apart from pork,
of course) as long as they pronounce God’s name at the time
of eating it. This opinion is based on a Koranic verse that
states that the food of Christians and Jews (with the excep-
tions noted above) is lawful for Muslims. Similar to kosher
food laws, pork and blood are prohibited. Unlike kosher laws,
Muslims can eat shellfish and certain animals without cloven
hooves (rabbit, for example).
Halal certification is a voluntary process by which a recog-
nized Islamic authority certifies that a company’s products can
be lawfully consumed by Muslims. Those who meet the criteria
for certification are given halal certificates, and they may use a
halal marking or symbol on their products and advertising.
As with the kosher symbol on food labels, the “halal certi-
fied” stamp on a label is seen by Muslim customers as a sign of
a trustworthy or superior product. Such a stamp is commonly
required for the export of certain foods to Muslim countries
such as Saudi Arabia and Malaysia. Products that are halal cer-
tified may be marked with a halal symbol, or simply the letter
M (as the letter K is used to identify kosher products).
Halal certification is similar to kosher. Each certifying
organization has its own procedures and requirements. In
general, however, products will be checked to ensure that:
• Raw materials, processing-aid ingredients, processing,
sanitation chemicals, and packaging meet the Islamic
dietary requirements, primarily that no alcohol or pork
products are used.
• Flavoring ingredients and solvents must be from halal
sources (no ethyl alcohol, for example).
• Meat products (non‐pork) have been slaughtered under
Islamic guidelines.
Food manufacturers pay a fee and voluntarily submit their
food products for halal certification. Independent organiza-
tions, under the auspices of a mullah (the Islamic equivalent
of the Jewish rabbi), are responsible for screening the prod-
ucts, observing the production process, and deciding on a
company’s compliance with Islamic dietary laws.
Vegetarianism
People become vegetarians for all sorts of reasons: ethical,
religious, health, economic, environmental—or a combina-
tion of factors. From annual polls, the Vegetarian Resource
Group (VRG) found that 2 to 3 percent of the U.S. population
claim to never consume meat, fish, or fowl and thus are strict
vegetarians. However, a Zogby Poll fielded in 1999 and 2008
found that in both years more than half the population some-
times, often, or always ordered meatless dishes. While there
are many types of vegetarians—from lacto‐ovo, who do not
eat meat, fish, or fowl but do eat eggs and dairy, to vegans,
who refrain from all meat, fish, fowl, eggs, dairy, and products
derived from these foods—a new group is emerging, known
as flexitarians. They are “semi‐vegetarians” whose main focus
is on decreased or occasional meat consumption. What does
this mean to the food industry? That manufacturers must
c16.indd 338 1/14/2016 12:50:48 PM
CHAPTER 16 • Developing Nutritious Food Products • 339
strive to keep their vegetarian offerings fresh, appealing, and
satisfying to all potential consumers, as possibly half the pop-
ulation will dine on them at least occasionally.
Animal Versus Plant Protein
The major nutrient often at risk in a vegetarian diet is protein.
Amino acids are the building blocks of proteins, and of the
20 common types, nine must be consumed in food (essential
amino acids); the rest can be produced by the body (nonessen-
tial amino acids). Almost all animal products are nutrient‐
dense sources of protein, also known as complete proteins,
because they contain an adequate amount of all the essential
amino acids. Conversely, most proteins found in plants lack a
sufficient amount of one or more essential amino acids and are
therefore deemed incomplete proteins. To compensate for the
deficiencies in essential amino acids, many plant proteins can
be combined to form complementary proteins.
For this reason, when preparing meals, vegetarians and
those producing vegetarian foods must be aware of the amino
acid composition of plant‐based foods and be able to com-
pensate for the essential amino acids that are lacking. For
instance, if a person eats only foods containing incomplete
proteins, such as those found in most vegetables and fruits,
and does not combine them properly, then his or her ability
to synthesize proteins in the body for growth and repair
slows or halts due to the limiting amino acid content. Instead,
the protein is broken down and utilized only as an energy
source (calories). The latest research shows that as long as
complementary foods are eaten throughout the day, the body
can use them to form complete proteins. It isn’t necessary to
eat them at the same meal.41,42 Note: The limiting amino acid
is the essential amino acid found in the smallest quantity in a
food. For more on plant proteins, see Chapter 7.
Complete Plant Proteins
In addition to developing vegetarian foods that feature comple-
mentary proteins, the product developer also has excellent
complete plant proteins to work with, notably soy and quinoa.
Soy
Soybeans are a versatile and dynamic legume high in protein
and fiber. When they are picked before maturity, they look
similar to sugar snap peas, with a bright green, fuzzy exterior
FIGURE 16.17 Complementary proteins.
Food Item Limiting Amino Acid
Foods High in Limiting
Amino Acids Complementary Food Combinations
Dried beans
and peas
Methionine and cysteine Grains, nuts, and seeds
(methionine and cysteine)
Brown rice and beans with peppers; bean
paste (beans, lemon, oil, and tahini)
Grains Lysine Dried beans and peas
(lysine)
Nut butter and whole wheat bread;
barley and 3‐bean soup; corn tortilla
and refried beans with salsa
Vegetables Lysine, methionine,
cysteine
Dried beans and peas (lysine);
grains, nuts, and seeds
(methionine and cysteine)
Vegetable stir‐fry with tofu and brown
rice; fresh greens with pine nuts and
beans
that encloses a sweet and tender pea (known as edamame, a
Japanese word meaning “twig bean”).
When soybeans are allowed to mature to their fully dry
state, they are typically processed into a wide variety of food
products including oil, tofu, soy sauce, flour, miso, and an array
of imitation dairy products. Most important for the product
developer are the soy protein isolates, concentrates, and flours
that are available to provide nutritional enhancement and
functional benefits (emulsification, texturization) to a wide
range of vegetarian and non‐vegetarian products as well as
an ever‐expanding menu of soy‐based foods such as salad
dressings, soups, imitation meats, beverage powders, cheeses,
nondairy creamer, frozen desserts, whipped topping, infant
formulas, breads, breakfast cereals, pastas, and pet foods.
FIGURE 16.18 Soybean pods.
ping han/Getty Images, Inc.
Source: Margaret Condrasky, Ed.D, RD, CCE.
c16.indd 339 1/14/2016 12:51:00 PM
340 • CULINOLOGY®
Soy protein isolate is a highly refined or purified form of
soy protein with a minimum protein content of 90 percent on
a dry basis. It is made from defatted soy flour, which has had
most of the non‐protein components, fats, and carbohydrates
removed. Because of this, it has a neutral flavor and causes
less flatulence due to intestinal fermentation of the carbohy-
drates that have been removed.
Soy isolates are mainly used to improve the texture of
meat products. In addition, they are also used to increase
protein content, enhance moisture retention, and as an
emulsifier.
Soy protein concentrate is about 70 percent soy protein
and is basically defatted soy flour without the water‐soluble
carbohydrates (mainly sugars). Soy protein concentrate
retains most of the fiber of the original soybean. It is widely
used as a functional or nutritional ingredient in many food
products, especially baked items and breakfast cereals. In
some meat and poultry products, soy protein concentrate is
used to increase water and fat retention or to improve nutri-
tional values (more protein, less fat).
Soy protein concentrates are available in several forms:
granules, flour, and spray‐dried. Because they are so digesti-
ble, they are well suited for children, pregnant and lactating
women, and the elderly. They are also used in pet foods and
as milk replacements for babies (both human and livestock).
Soy flour is made by grinding soybeans into a fine powder.
It comes in three forms: natural or full fat (contains natural
oils); defatted (oils removed) with 50 percent protein content
and either high or low water solubility; and lecithinated (leci-
thin added to enhance its emulsification properties). As soy
flour is gluten‐free, yeast‐raised breads made with soy flour
are dense in texture.
Textured soy protein (TSP), also known as textured vegeta-
ble protein (TVP), is a meat analog or meat extender made
from defatted soy flour. It is quick to cook, with a protein con-
tent equal to that of the meat. TSP is made by processing a
dough made from high‐water‐soluble defatted soy flour in a
screw‐type extruder under heat and pressure. The dough is
extruded through a die into various shapes—chunks, flakes,
FIGURE 16.19 Soy products.
Diane Labombarbe/Getty Images, Inc.
nuggets, strips, and so on—and dried in an oven. The extrusion
process changes the structure of the soy protein, resulting in a
fibrous, spongy matrix similar in texture to meat. TSP made
from soy flour contains 50 percent soy protein and must be
rehydrated before use at a weight ratio of 1:2 TSP to water.
(However, TSP, when made from soy concentrate rather than
soy flour, contains 70 percent protein and is rehydrated at a
ratio of 1:3.) Rehydrated TVP is commonly used as a ground
meat extender (at up to one‐third the weight of the meat) due
to its low cost—less than a third the price of ground beef—and
when cooked together with meat helps retain yield by absorb-
ing juices normally lost. Extension may result in diminished
flavor, but fat and cholesterol are reduced.
Textured vegetable protein is a versatile substance whose
different forms allow it to take on the texture of whatever
ground meat it is substituting for. Using textured vegetable
protein, one can make vegetarian or vegan versions of tradi-
tional meat dishes such as sausages, chili, spaghetti Bolognese,
sloppy joes, tacos, burgers, and burritos.
Quinoa
Quinoa is a plant containing a balanced set of essential amino
acids for humans, making it a complete protein source. In
addition, it is a good source of dietary fiber and phosphorus
and is high in magnesium and iron. Quinoa is gluten‐free and
is considered easy to digest. Because of all these characteris-
tics, quinoa is being considered a possible crop in the National
Aeronautical and Space Administration’s (NASA) Controlled
Ecological Life Support System for long‐duration manned
spaceflights.43
Quinoa contains more protein than any other grain and is
also higher in unsaturated fat and lower in carbohydrates
than other grains. This tiny, bead‐like grain cooks like rice but
in half the time and expands to four times its original size.
Quinoa is available in several colors (tan, red, and black)
and can be used like rice in main dishes, side dishes, salads, and
soups. It is found packaged as a grain, ground into flour,
and in several forms of pasta.
Eating a More Vegetarian Diet
Vegetable proteins are heart‐healthy alternatives to animal
proteins because they contain no cholesterol and little satu-
rated fat as well as a high amount of fiber, vitamins, minerals,
and phytochemicals, which may aid in the prevention of
chronic diseases. They are inexpensive, versatile, and tasty;
add flavor, color, and texture to the diet; and benefit health
beyond their contribution of protein. Therefore, trimming
the meat portion of any meal and filling the void with an
array of nuts, grains, legumes, and vegetables is a good idea
for non‐vegetarians and vegetarians alike.
Healthy Cooking Approaches
and Diets
The USDA’s Food Guide Pyramid was designed to create a
healthier America. Unfortunately, since its inception the obe-
sity rate has continued to rise, not only in the United States
c16.indd 340 1/14/2016 12:51:09 PM
CHAPTER 16 • Developing Nutritious Food Products • 341
but around the world as more people have begun to follow a
Western diet. Today, with 4 of the top 10 causes of American
deaths (heart disease, cancer, stroke, and diabetes) related to
poor eating behavior,44 other groups and agencies are offering
new ideas on how to eat a balanced meal.
Diabetic Diets
According to the CDC, nearly 24 million Americans have dia-
betes, and about 6 million of them are unaware of their dis-
ease.45 Therefore, the significance of diabetes awareness and
prevention is crucial. Diabetes is a disease in which the body
does not produce or properly use insulin. While the exact
cause of diabetes is not completely understood, genetics and
environmental factors such as obesity and lack of exercise
play major roles. The American Diabetes Association (ADA)
lists four major types of diabetes:
1. Type I diabetes (formerly known as juvenile diabetes; it is
now known that Type I can strike at any age) is a genetic
condition that results from the body’s failure to produce
insulin (or an insufficient amount). Insulin is the hormone
that allows glucose into the cells to fuel them; 5 to 10 per-
cent of Americans who are diagnosed have Type I diabetes.
This type requires the person with diabetes to take daily
injections of insulin in order to survive.
2. Type II diabetes (also known as adult‐onset diabetes)
results from insulin resistance, a condition in which the
body fails to properly use insulin, often combined with an
insulin deficiency; most Americans who are diagnosed
have Type II diabetes. This type is associated with over-
weight, non‐exercising individuals.
3. Gestational diabetes occurs during or immediately after
pregnancy; 5 to 10 percent of women with gestational dia-
betes are found to have Type II diabetes.
4. Pre‐diabetes is a condition that occurs when a person’s
blood glucose levels are higher than normal but not high
enough to be diagnosed with Type II diabetes; in addition
to the 23.6 million with diabetes, 57 million Americans
have pre‐diabetes, and are at risk of developing full-blown
disease if they ignore the warnings and don’t make serious
lifestyle and dietary changes.46
It is important to note that people with diabetes have sim-
ilar nutritional needs as other consumers. In fact, exercising
and eating well‐balanced meals are essential not just in the
management of diabetes but also in the maintenance of gen-
eral good health.
The Carbohydrate Countdown
The major consideration for people with diabetes is monitor-
ing carbohydrate intake. Examples of foods that contain car-
bohydrate are:
• Grains and flour‐containing foods—bread, cereal, rice,
crackers
• Sugars and sugar‐containing foods—fruit, fruit juices
• Dairy products—milk, yogurt (entirely lactose)
• Beans and legumes as well as soybean products—veggie
burgers
• Sweets and snack foods—sodas, cake, cookies, candy,
chips
• Starchy vegetables—potatoes and corn
Many people with diabetes follow a meal plan, such as
using diabetic exchange lists or so‐called “carb counting.”
Diabetes nutrition experts are against meal plans that elimi-
nate healthful foods such as legumes, whole grains, fruits,
and vegetables because these foods are associated with lower
risk for developing other chronic conditions, such as heart
disease and some cancers.47 According to the ADA, research
shows that low‐fat and moderate‐ to low‐carbohydrate meal
plans prove effective in controlling Type II diabetes, often to
the point where medication is not required.
Creating a Diabetic‐Friendly Plate
Every 24 hours, more than 4000 adults are diagnosed with
diabetes, and 200 die from it. New food products are of dire
necessity not only for the control of diabetes but also for its
prevention. To help in the creation of diabetic‐friendly foods,
the ADA created the “Create Your Plate” method, a simple
and effective way to plate up healthy food choices focusing
on the consumption of non‐starchy vegetables. (Note: ADA’s
Create Your Plate concept is similar to, but not the same as,
the USDA’s MyPlate food guide.)
ADA’s Create Your Plate Method
1. Draw an imaginary line down the middle of the plate.
2. On one side, cut it again for a total of three sections on the
plate.
3. Fill the largest section with non‐starchy vegetables.
4. Put starchy foods in one of the small sections.
5. Put a lean meat or meat substitute in the other small section.
6. Add an 8‐ounce glass of nonfat or low‐fat milk. Alternatively,
add another small serving of carbohydrate, such as a
6‐ounce container of light yogurt or a small roll.
7. Add a piece of fresh fruit or ½ cup fruit salad.
This plate technique is not limited to people with diabe-
tes; it is a valuable tool for all concerned with creating meals
composed of healthier food choices in the appropriate
amounts. For further information on diabetes and Create
Your Plate, visit the ADA website.
The New American Plate
The American Institute for Cancer Research (AICR) created
the “New American Plate,” which, similar to the ADA’s plate,
is divided into thirds—two thirds hold vegetables, fruits,
whole grains, and beans; and one third holds animal protein.
The New American Plate is a simplified meal plan that
focuses on plant‐based foods that lower the risk for cancer
and other chronic diseases while managing weight. Plant‐
based foods provide an array of cancer‐protective com-
pounds; in addition, a predominantly plant‐based diet is a
c16.indd 341 1/14/2016 12:51:09 PM
342 • CULINOLOGY®
powerful tool in weight management. In fact, the fiber and
water in plant foods gives a feeling of fullness without supply-
ing a lot of calories. The three strategies for both cancer pre-
vention and weight loss, as constructed by the AICR, are:
1. Eat mostly plant‐based foods, which are low in energy density.
2. Be physically active.
3. Maintain a healthy weight through reduced portion size.
Hypertension (The DASH Diet)
Obesity leading to diabetes and heart disease is one of the
leading causes of illness and death in the United States.
Hypertension, or high blood pressure, is one of the leading
early indicators of risk. The DASH (Develop Approaches to
Stop Hypertension) diet achieves significant success for
patients seeking to reduce hypertension without medication.
In addition to being a low‐salt (or low‐sodium) plan, it is rich
in fruits and vegetables, low‐fat or nonfat dairy, and whole
grains. It is a high‐fiber, low‐ to moderate‐fat diet, rich in
potassium, calcium, and magnesium. The DASH diet eating
plan has proven to lower blood pressure in studies sponsored
by the National Institutes of Health.
The DASH diet received a #1 ranking from U.S. News &
World Report.48 DASH is also endorsed by:
• The National Heart, Lung, and Blood Institute (one of
the National Institutes of Health, part of the HHS)
• The American Heart Association (AHA)
• The 2010 Dietary Guidelines for Americans
• U.S. Guidelines for Treatment of High Blood Pressure
• The 2011 AHA Treatment Guidelines for Women
• The Mayo Clinic
In addition, the DASH diet formed the basis for the USDA’s
MyPlate.
Allergens
Every year, food allergies are responsible for approximately
200,000 severe allergic reactions requiring hospitalizations and
result in over 200 deaths.49 Only eight foods account for 90 per-
cent of all food allergy reactions: cow’s milk, eggs, peanuts, tree
nuts (such as pecans, almonds, and walnuts), fish (such as bass,
cod, and flounder), shellfish (such as crab, lobster, and shrimp),
soy, and wheat.50 Although allergies to milk, egg, wheat, and soy
generally resolve in childhood, they appear to be resolving
more slowly than in previous decades, with many children still
allergic beyond the age of five. Allergies to peanuts, tree nuts,
fish, and shellfish are generally lifelong. In all cases, allergens
are protein‐based. Refined oils are not allergenic, even if they
were derived from allergenic foods (peanut oil, soybean oil).
A study published by the National Center for Health
Statistics division of the CDC reports that one out of every 25
children in the United States has a food allergy, representing
about a 50 percent increase between 1997 and 2011.51 The
prevalence of food allergy may be increasing in the United
States and in other countries.52 Studies are inconclusive about
whether food allergies can be prevented. No medication can be
taken to prevent food allergies. They are a topic of concern for
people not only in the healthcare industry but also in the food-
service sector. With as many as 15 million Americans having
food allergies (60 percent adults, 40 percent children), this is an
expanding market that must be fed carefully.
An allergy is a reaction to a food that involves the body’s
immune system. The body produces an immune response by
releasing histamine, which generates the allergic symptoms.
These range in severity from skin rashes, breathing problems,
vomiting, and cramping, to swelling of the tongue and throat.
A severe reaction (anaphylactic shock) can result in death.
Life‐threatening allergic reactions at home can be treated
with the prescription drug epinephrine, usually delivered via
injection using an Epi‐Pen®. People with food allergies must
carefully assess and evaluate all processed foods, read ingre-
dient labels on packages, and alert servers about any allergies
(if they don’t ask you first) before ordering anything when
eating out. Avoidance of allergy‐causing foods is the only way
to avoid a reaction; there is no cure. Unfortunately, many of
the allergy‐causing foods are used as ingredients in other
foods (for example, milk and eggs in baked goods, nuts in
confections). This makes recognizing the presence of food
allergens and their sources of critical importance to food
product developers in order to create safer alternatives.
Allergen Labeling
The Food Allergen Labeling and Consumer Protection Act
(FALCPA) requires food manufacturers to list the “Big Eight”
food allergens on labels. While not every possible food that
can cause a food allergy is listed, these eight food categories
account for most food allergy reactions.
Fresh produce, fresh meat, and certain highly refined oils
do not require listing of potential food allergens on the labels.
Foods that may inadvertently come into contact with a food
allergen during the growing, harvesting, or manufacturing
process also are exempt. However, many manufacturers rou-
tinely include warnings on their labels of the possibility that
trace amounts of allergy‐causing foods may be present as a
way to stave off liability. FALCPA does not regulate the use of
advisory or precautionary labeling (for example, “prepared in
a facility that also processes nuts”); this is voluntary on the
part of the manufacturer. These terms do not reflect specific
risks; in fact, random testing for allergens of products bearing
such warning labels shows a range of results from none to
amounts that can cause reactions.53,54 Further, these warn-
ings do not provide any legal defense for the manufacturer in
case a lawsuit should arise.
Food Intolerances
Food allergies are different from food intolerances. Unlike an
allergy, a food intolerance does not involve an immunologic
reaction. Perhaps the best‐known type is lactose intol erance.
People with lactose intolerance lack the enzyme lactase, required
to properly digest the milk sugar lactose. As a result, they experi-
ence gas, bloating, and abdominal pain when they consume
c16.indd 342 1/14/2016 12:51:09 PM
CHAPTER 16 • Developing Nutritious Food Products • 343
FIGURE 16.20 Sample USDA food guide and the DASH eating plan at the 2000‐calorie level.
Food Groups and Subgroups DASH Eating Plan Amounta Equivalent Amounts
Fruit Group 2 to 2.5 cups (4 to 5 servings) ½ cup equivalent is:
• ½ cup fresh, frozen, or canned fruit
• 1 medium fruit
• ¼ cup dried fruit
• ½ cup fruit juice
Vegetable Group
• Dark green vegetables
• Orange vegetables
• Legumes (dry beans)
• Starchy vegetables
• Other vegetables
2 to 2.5 cups (4 to 5 servings) ½ cup equivalent is:
• ½ cup of cut‐up raw or cooked vegetable
• 1 cup raw leafy vegetable
• ½ cup vegetable juice
Grain Group
• Whole grains
• Other grains
6 to 8 ounce equivalents
(6 to 8 servingsb)
1 ounce equivalent is:
• 1 slice bread
• 1 cup dry cereal
• ½ cup cooked rice, pasta, cereal
• DASH: 1 oz dry cereal (½–1¼ cups, depend-
ing on cereal type—check label)
Meat and Beans Group 6 ounces or less meats, poultry, fish 1 ounce equivalent is:
• 1 ounce of cooked lean meats, poultry, fish
• 1 eggd
• USDA: ¼ cup cooked dry beans or tofu,
1 Tbsp peanut butter, ½ oz nuts or seeds
• DASH: 1½ oz nuts, 2 Tbsp peanut
butter, ½ oz seeds, ½ cup cooked dry beans
4 to 5 servings per week nuts,
seeds, and legumesc
Milk Group 2 to 3 cups 1 cup equivalent is:
• 1 cup low‐fat/fat‐free milk, yogurt
• 1½ oz of low‐fat, fat‐free, or reduced fat
natural cheese
• 2 oz of low‐fat or fat‐free processed cheese
Oils 8 to 12 grams (2 to 3 tsp) DASH: 1 tsp equivalent is:
• 1 tsp soft margarine
• 1 Tbsp low‐fat mayo
• 2 Tbsp light salad dressing
• 1 tsp vegetable oil
Discretionary Calorie
Allowance
• Example of distribution:
solid fat,e added sugars
~2 tsp of added sugar
(5 Tbsp per week)
DASH: 1 Tbsp added sugar equivalent is:
• 1 Tbsp jelly or jam
• ½ cup sorbet and ices
• 1 cup lemonade
aAll servings are per day unless otherwise noted. USDA vegetable subgroup amounts and amounts of DASH nuts, seeds, and dry beans are
per week.
bWhole grains are recommended for most grain servings to meet fiber recommendations.
cIn the DASH Eating Plan, nuts, seeds, and legumes are a separate food group from meats, poultry, and fish.
dBecause eggs are high in cholesterol, limit egg yolk intake to no more than 4 per week; 2 egg whites have the same protein content as
1 oz of meat.
eThe oils listed in this table are not considered to be part of discretionary calories because they are a major source of the vitamin E and
polyunsaturated fatty acids, including the essential fatty acids, in the food pattern. In contrast, solid fats (i.e., saturated and trans fats) are
listed separately as a source of discretionary calories.
Note: Table updated to reflect 2006 DASH Eating Plan.
Source: HHS/USDA Dietary Guidelines (2005).
milk products. Lactose intolerance can be treated by consuming
lactase tablets and, possibly, by gradually increasing intake of fer-
mented dairy foods such as yogurt or aged cheeses. Most, if not
all, of the lactose in these products is broken down during fer-
mentation and aging (in cheeses) by microorganisms.
Food sensitivities are similar to intolerances. Some peo-
ple’s bodies are not able to tolerate caffeine, while others are
sensitive to or intolerant of alcohol because of the missing
enzyme alcohol dehydrogenase (ADH). It is important to
know the difference between a food allergy and an intolerance
c16.indd 343 1/14/2016 12:51:09 PM
344 • CULINOLOGY®
because even the smallest amount of a given food can trigger
a severe reaction in people with allergies, while people with
food intolerances can usually eat small amounts of that food
without fear of hospitalization (or worse).
Gluten Sensitivity
Another food intolerance that is garnering a lot of food indus-
try attention is gluten intolerance in individuals who cannot
digest the cereal protein gluten, found primarily (but not
exclusively) in wheat‐based products. This is not an allergy ;
consuming gluten does not trigger the release of histamine.
Gluten sensitivity is increasing and can cause a serious
reaction in people who have celiac disease (also known as
gluten‐sensitive enteropathy), an autoimmune inflammatory
disease of the small intestine that is triggered by the con-
sumption of gluten. In fact, recent studies in the United States
suggest the prevalence of celiac disease is approximately
1 case per 250 persons.55
People with a family history of celiac disease or personal
history of Type I diabetes have an increased risk for acquiring
celiac disease. In younger children, gluten damages the villi,
the finger‐like projections in the small intestine responsible
for absorbing nutrients. When the villi are damaged, the body
cannot absorb nutrients and malnutrition can result. Gluten
sensitivity can lead to varying symptoms in different people,
including diarrhea, abdominal pain and bloating, weight loss,
fatigue, and painful skin rashes.
By removing gluten from one’s diet, the symptoms caused
by celiac disease subside. Today, many types of gluten‐free
products are readily available, such as breads, cookies, and
cakes. Gluten‐free flour, also easily obtained, is usually made
by blending flours from non‐gluten‐containing vegetable
sources such as potatoes, legumes, rice, nuts, and seeds, with
natural gums (hydrocolloids) added to provide the functional
properties of the missing gluten. In other words, being glu-
ten‐free is a lot easier than it used to be.
A huge industry has grown up in recent years around sup-
plying gluten‐free versions of such foods as cereals, breads
and baked goods, pasta, and other traditionally gluten‐con-
taining foods. Globally, gluten‐free food sales were nearly $5
billion in 2012, with the United States accounting for 59 per-
cent of the total market.56 The USDA projects that the gluten‐
free industry’s revenues will continue to soar.57
The FDA, as of August 2013, defined gluten‐free as contain-
ing less than 20 parts per million (ppm) of gluten. Food manu-
facturers are legally allowed to label a food “gluten‐free” even if
that food contains an ingredient made from wheat, barley, or rye
(or a crossbreed from those grains) as long as it is processed to
remove the gluten to the allowable level of 20 ppm. The rule also
requires foods with the claims “no gluten,” “free of gluten,” and
“without gluten” to meet the same definition as “gluten‐free.”
This new federal definition standardizes the meaning of “gluten‐
free” claims across the food industry and provides a uniform
standard definition to help the up to 1.5 million Americans with
celiac disease. The FDA recognizes that many foods currently
labeled “gluten‐free” already meet the new federal definition.58
Note: The labels are voluntary. Manufacturers wishing to
cater to gluten‐free consumers may choose to add the claim
to their packaging, but they aren’t required to do so. The
labels do not eliminate the need to identify gluten‐containing
foods on labels, as the FDA’s rules do not require manufactur-
ers to disclose gluten‐containing ingredients beyond their
standard name: wheat, barley, and so on.
Grain Products Without Gluten
• Buckwheat
• Corn and products made from corn: grits, tortillas, pop-
corn, polenta, taco shells, tortilla chips, corn chips
• Millet
• Rice and products made from rice (rice cakes, rice crack-
ers, rice noodles)
• Quinoa
Note: Check the ingredient labels carefully; seasonings added
to these products may include gluten‐containing ingredients.
Culinary Nutrition News
With all kinds of new information, research, and applica-
tions about food and nutrition coming out every day, a lot
of buzzworthy food items and ingredients are making the
headlines.
Processed Meats Cause Cancer?
The shocking headline from a recently released report from
the World Health Organization (WHO) says it all: Eating
processed meats such as sausages, bacon, and ham causes
cancer, while unprocessed red meat may also be carcinogenic.
The WHO’s cancer research unit now classifies proc essed
meat as “carcinogenic to humans” based on evidence from
hundreds of studies, and linked it specifically to colon cancer.
The organization defines processed meat as any type of
meat that is salted, cured, or smoked to enhance its flavor or
preserve it. Processed meat generally contains pork or beef,
but may also contain poultry.
The report states that eating just 50 grams of processed
meat each day—the equivalent of two slices of ham—can
increase the risk of colon cancer by 18 percent, and risk increa ses
with increased consumption. Processed meat will now be
placed in the same category as smoking and asbestos, based
on the certainty of a link with cancer. However, the authors
stressed that this does not mean that eating processed meat
is equally dangerous; compared with smoking and exposure
to asbestos, the risks from eating processed meats are rela-
tively small. Unprocessed red meats such as beef and lamb
were also implicated, but to a much lesser extent.
That said, California is now considering adding processed
meats to its Proposition 65 cancer‐alert list. California’s “Prop
65,” an initiative approved in 1986, requires that the state keep
a list of all chemicals and substances known to increase cancer
risks. Producers of such products are required to provide “clear
and reasonable” warnings for consumers (usually in the form of
a warning label on implicated foods). Typically, once an item is
c16.indd 344 1/14/2016 12:51:09 PM
CHAPTER 16 • Developing Nutritious Food Products • 345
added, it is up to the producer to prove to the state that its
product is not dangerous enough to warrant a warning label.
As would be expected, the American meat industry is
actively lobbying against this measure.
Organic
Once upon a time, one would not think twice about buying a
fresh, ripe apple with a worm hole. Many people merely ate or
cut around the opening, but those days are long gone. Once
familiar, food imperfections have become unacceptable in the
eyes of consumers. Because of reductions in crop yields or
product quality due to pest infestation or competition from
weeds, farmers began relying more heavily on pesticides. With
the discovery of the potentially harmful health and environ-
mental effects of these chemicals and the advent of the envi-
ronmental movement, consumers and members of the medical
and scientific communities began to voice opposition to the
use of these chemicals. Concerned farmers sought another
solution, which is how organic farming evolved. However, the
fight over what is safe and good for people and the environ-
ment is an ongoing battle, with both sides standing firm.
Conventional Farming Versus Organic Farming
While the common assumption is that conventional farming
using pesticides is dangerous and organic farming is safe, there
are pros and cons to both. Pesticides used in food production
are believed to help ensure a safe and adequate food supply and
make foods available at a reasonable cost. In fact, many farmers
claim that without them it would be impossible to stay in busi-
ness because they would lose too much of their crop to make a
profit. Unfortunately, chemical pesticides are highly toxic and
nonspecific—that is, they destroy pests but also harm non‐
target organisms such as humans, wildlife, and beneficial
organisms in the environment. Further, they tend to persist for
years in both soil and water. There are growing concerns that
pesticides may be linked to increased cancer rates, decreased
reproductive capability (in humans and wildlife), a wide assort-
ment of mutations and animal extinctions, contamination of
water supplies, and poisoning of wildlife habitats.
The alternative, organic farming, stresses the use of renew-
able resources, such as crop rotation, green manure, compost,
biological pest control, and the conservation of soil and water,
to enhance the quality of the environment. Organic foods are
produced without antibiotics, hormones, synthetic fertilizers
and pesticides, sewage sludge, genetic modifications, and
spoilage‐inhibiting radiation. Organic foods are often consid-
ered healthier because they are processed without artificial
ingredients, preservatives, or irradiation, and environmentally
friendly because soil nutrition is maintained naturally. Al-
though organic farming techniques can benefit the environ-
ment, crop yields are typically lower than those conventionally
grown. Lower crop yields paired with the laborious and time‐
intensive systems used by organic farms leads to more expen-
sive products; therefore, consumers must decide if the potential
benefits of the products are worth the extra cost.
Organic Foods and the Government
Today, many organic options are available, from fresh pro-
duce and meats to dairy products and cereals, to many types
of processed foods. To protect consumers, the USDA regu-
lates the production and labeling of organic foods. According
to these rules, a food product cannot be labeled “organic”
unless its production meets strict standards. For labeling
purposes, certified organic food manufacturers can use the
circular “USDA Organic” symbol on the package.
The USDA National Organic Program (NOP) defines
organic as follows:
Organic food is produced by farmers who emphasize the use of
renewable resources and the conservation of soil and water to
enhance environmental quality for future generations. Organic
meat, poultry, eggs, and dairy products come from animals that
are given no antibiotics or growth hormones. Organic food is
produced without using most conventional pesticides; fertiliz-
ers made with synthetic ingredients or sewage sludge; bioengi-
neering; or ionizing radiation. Before a product can be labeled
“organic,” a Government‐approved certifier inspects the farm
where the food is grown to make sure the farmer is following
all the rules necessary to meet USDA organic standards. Com-
panies that handle or process organic food before it gets to your
local supermarket or restaurant must be certified, too.59
For more information about the government’s organic food
standards, visit the USDA’s National Organic Program’s website.60
Natural (Not Necessarily Organic) Foods
Natural foods may not contain additives or preservatives;
however, they may contain ingredients that are genetically
modified or even grown with pesticides. Although the ingre-
dients on the food label may look familiar, natural foods are
not regulated and do not have to meet the same criteria as
organic foods. The USDA defines “natural” as:
FIGURE 16.21 Organic label.
Source: USDA.
c16.indd 345 1/14/2016 12:51:10 PM
346 • CULINOLOGY®
A product containing no artificial ingredient or added color
and is only minimally processed (a process which does not fun-
damentally alter the raw product) may be labeled natural. The
label must explain the use of the term natural (such as—no
added colorings or artificial ingredients; minimally processed).61
All‐Natural
Natural and organic trends have been growing in popularity
for some time now. Yet, what exactly does it mean to be “all‐
natural”? Increased consumer perception of the possible
negative effects of artificial colors, flavors, and preservatives
has changed the scope of food product development. We now
see food companies switching from the artificial sweeteners
and colors on which they once relied heavily to more natural
alternatives. Now we are seeing the ingredient list shrink to
the essential, supposedly natural ingredients.
Many food companies are jumping on the all‐natural band-
wagon to promote their products. For example, a major bever-
age company recently introduced a line of all‐natural sodas,
meaning simply that they have been reformulated with natural
sugar to replace high‐fructose corn syrup (HFCS). But does that
make the soda any healthier? It is important to note that both
sugar and HFCS provide the same number of calories per gram.
While the FDA has no formal definition of “all‐natural,” it has not
objected to the use of the term for products that contain no arti-
ficial colors or flavors or synthetic substances. The USDA, which
regulates meat and poultry, defines as natural those products
that are minimally processed and contain no artificial flavors or
colors, or synthetic ingredients or chemical preservatives.
One unforeseen consequence to the food industry has been
a plethora of lawsuits over the past few years (and several mul-
timillion dollar payouts) launched by litigious groups of plain-
tiffs (and their lawyers) claiming that food and beverage
manufacturers are trying to pull the wool over consumer’s
naïve eyes by including ingredients in their labeled “all‐natural”
products that aren’t exactly all‐natural. Huge multinational
companies including FritoLay, General Mills, Unilever, Kellogg’s,
Campbell Soup, and many others are being sued for using
such ingredients as alkalized cocoa, citric acid, ascorbic acid,
maltodextrin, caramel color, even high-fructose corn syrup
in their supposedly “all‐natural” products. At question is
whether these ingredients can truly be considered natural,
when none of the manufacturing processes used to make them
are “found in nature.” As the lawsuits pile up, more and more of
these companies are dropping “all‐natural” entirely from their
product labels and advertising.
But even if the manufacturers stop using the term all‐natural
to describe their products, the trend toward fewer, healthier
and more familiar ingredients seems to “have legs.” This trend
is even seen in traditionally unhealthy snack foods such as
chips, crackers, and cakes. That said, consumers are notorious
trend followers, and after the initial glow wears off, they may go
back to their cheaper, “better tasting” old favorites (remem-
ber the uproar when the Twinkies bakeries closed down?). In
addition, the natural, all‐natural, and organic movements
have not stemmed the tide of obesity one iota. Overeating
organic or all‐natural foods can still make you fat!
Functional Foods
There is an ongoing debate between food manufacturers and
consumer advocates about the promotion of what are called
functional foods. Functional foods are any that provide health
benefits beyond basic nutrition. While all foods are func-
tional in that they provide nutrients, foods considered
functional are formulated to supply one or more dietary
ingredients that may improve health, or they contain high
levels of substances that tend to prevent certain diseases.
What qualifies foods as functional? Sodas with added vita-
mins, calcium‐fortified juices and soy milks, yogurts with
specialized strains of bacteria, and many other enhanced
foods or vitamin‐fortified products constitute a large portion
of the functional food market. In any U.S. supermarket one
finds energy bars, breakfast cereals, bottled waters, sports
and energy beverages, herbal teas, breads, and soups spiked
with vitamins, minerals, and botanicals (taurine, valerian, St.
John’s wort, and so on). Also, foods rich in phytochemicals are
now part of the functional food family, such as processed
tomato products that contain the phytochemical lycopene.
Every food has its function; however, not every food is a
functional food, according to the ADA, which defines func-
tional foods as “whole foods and fortified, enriched, or
enhanced foods that have a potentially beneficial effect on
health when consumed as part of a varied, regular diet. They
are considered to contain a healthful food or food ingredient
that may provide a health benefit beyond the traditional
nutrients.”62 The term functional implies that the food has
some identified value leading to health benefits, including
reduced risk for disease, for the person consuming it.63
Unmodified whole foods, such as fruits and vegetables,
represent the simplest form of functional foods. For example,
broccoli, carrots, and tomatoes are considered functional
foods because they are rich in physiologically active compo-
nents such as sulforaphane, beta carotene, and lycopene,
respectively.64 Modified foods, including those fortified with
nutrients or enhanced with phytochemicals or botanicals,
may also be considered functional foods.65 The term func-
tional food should not be used to imply that there are good
foods and bad foods, as all foods can be incorporated into a
healthful eating plan—the key being moderation and variety.66
Prebiotics and Probiotics
Prebiotics and probiotics are getting more attention in the food
world and now make up a class of functional foods by them-
selves. Prebiotics are non‐digestible carbohydrates that stimu-
late the growth or activity of beneficial bacteria in the colon;
these may help preserve intestinal health by preventing patho-
gens from gaining a foothold, maintaining digestive regularity,
and aiding in the prevention of colon cancer while decreasing
blood triglycerides, glucose, and insulin levels. The most preva-
lent forms of prebiotics are nutritionally classed as soluble fiber.
Probiotics, which means “for life” or “pro‐life,” are living
organisms that provide health benefits when consumed in
adequate amounts. Probiotics, also known as “friendly” bac-
teria, enter the body via consumption of fermented foods or
c16.indd 346 1/14/2016 12:51:11 PM
CHAPTER 16 • Developing Nutritious Food Products • 347
through the taking of microbial supplements to help colonize
the colon with microorganisms that help protect and heal the
colon lining. Friendly bacteria are vital to proper develop-
ment of the immune system, protection against pathogens,
and enhanced digestion as well as absorption of nutrients.
The probiotic strains most frequently employed in foods and
supplements are the Lactobacillus and Bifidobacterium spe-
cies. Common probiotic‐containing foods are yogurt and
kefir, unpasteurized pickles, sauerkraut and kimchi, miso
soup, olives, and dark chocolate. Dehydrated probiotic pure
cultures are also available in tablet form to use as dietary
supplements.
FIGURE 16.22 Examples of prebiotic and
probiotic foods.
Prebiotics
Chicory
Jerusalem artichokes
Wheat
Barley
Rye
Onions
Garlic
Leeks
Probiotics (actual bacteria found in given foods)
Yogurt with live culture
Buttermilk
Cottage cheese
Dairy spreads with inulin
Soy sauce
Tempeh
Fresh pickles and sauerkraut
Miso
The importance of the bifidobacteria and the lactic acid
bacteria (also called lactobacillus or LABs) is that they have
several beneficial effects on the host, especially in terms of
improving digestion (including enhancing mineral absorption)
and the effectiveness and intrinsic strength of the immune
system. Because, generally speaking, the American diet is
lacking in soluble fiber, prebiotics are increasingly added to
processed foods—including yogurts, cereals, breads, biscuits,
milk desserts, nutritional supplement bars, ice creams, spreads,
drinks, water, and infant formula, as well as to some ani-
mal foods—for their health benefits.
This is an excellent opportunity for the food product
developer working on healthy food projects. While there are
no legal definitions for an ingredient to be considered a prebi-
otic, it must meet the following terms:
• It is not broken down and absorbed in the body.
• It is selectively fermented by the good bacteria (probiot-
ics) in the gastrointestinal tract.
• It stimulates the growth of these good bacteria in the gut.
Among the trillions of bacterial cells in the human body, it
is estimated that there are only around 1000 varieties.67 Some
of these have a vital role in maintaining human health, while
others have negative impacts. One main role of probiotics
is to create a living barrier that prevents damaging bacteria
and viruses from multiplying inside the body. It does this
by increasing the acidity of the intestinal environment to
create less desirable living conditions for potential pathogens.
Probiotic organisms are like microscopic defenders that pro-
tect your body from invading organisms.68 Probiotics offer
other functions besides defense, such as:
• Breaking down food for easier digestion of certain
nutrients.
• Producing the lactase enzyme (which helps digest milk
sugar).
• Producing vitamins in the intestinal tract.
Prebiotics and probiotics are assumed to be safe because
some probiotic foods, such as fermented foods and cultured
milk products, date to ancient times. Lactobacillus plantarum
is one of the lactic acid–producing bacteria used for centuries
Bioactive Ingredients
Terpenoids Phenolics Carbohydrates Lipid BasedProtein or
Amino Acid
BasedTerpenes
Carotenoids
Tocopherols
Tocotrienols
Flavanols
Flavonones
Isoflavones
Tannins
Lignin
Anthocyanins
Polyphenols
Phenolic acids
Oligisaccharides
Cellulose
Pectin
Glucans
Resistant starch
Amino acids
Polypeptides
Choline
AllyI-S compounds
N-3 PUFAs
MUFAs
Lecithin
Sphingolipids
FIGURE 16.23 Categories of bioactive compounds.
c16.indd 347 1/14/2016 12:51:11 PM
348 • CULINOLOGY®
FIGURE 16.24 Bioactive components in foods, sources, and purported benefits.
Bioactive Component Sources Benefits
Soy foods Soybeans, soy protein Reduce risk of CHD (coronary heart disease)
Daidzein, Genestein Soy Menopausal health, immune function,
bone health
Carotenoids
β‐Carotene Citrus, carrots, pumpkin, squash Free radical scavenger, converted to Vitamin
A in vivo
Lutein, Zeazanthin Spinach, corn, eggs, citrus Antioxidant, eye health
Lycopene Tomatoes, watermelon Antioxidant, prostate health
Dietary fats (fatty acids)
Monounsaturated fatty acids Olive oil, canola oil Reduced risk of CHD
Conjugated linoleic acid Beef, lamb, dairy products Immune function and improved body
composition
Omega‐3 fatty acids Fish and fish oils Reduced risk of CHD, improved mental
acuity, vision
Dietary fiber and prebiotics
Insoluble fiber Cereal bran (wheat and corn) Digestive health, cancer prevention
Beta‐glucans Oatmeal, oat bran, barley Reduces CHD risk
Soluble fiber Psyllium, beans, apples Reduces CHD risk
Whole grains Wheat, brown rice, cereals Reduces CHD risk, controls serum glucose
levels
Inulin and fructo-
oligosaccharides
Whole grains, fruits, garlic,
onions
Improved immune function, gastrointestinal
health, mineral absorption
Probiotics Yogurt, culture dairy Immune function, GI health
Sulfur compounds
Allyl sulfides Onions, leeks, garlic Immune function, heart health
Dithiothionones Cruciferous vegetables Immune function and detoxification
Sulforaphane Broccoli, cauliflower, kale Cellular antioxidant, detoxification
Phytosterols and satanols Soy, wood, fortified table spreads Reduce LDL cholesterol
Flavonoids
Anthocyanins: Cyanidin,
Malvidin, Delphinidin
Cane berries, cherries, grapes,
red wine
Antioxidant defenses
Flavone‐3‐ols,
Catechins, Epicatechins,
Procyanidins
Tea, chocolate, apples Heart health, anti‐inflammation
Flavonols: Quercetin,
Kaempferol
Apples, broccoli, tea, onions,
blueberries, red wine
Scavenge free radicals, anti‐oxidant,
anti‐inflammatory
Pronathocyanidins Cranberries, apples, strawberries,
wine, cinnamon
Heart health, antioxidants, urinary
tract health
Flavones:
Luteolin, Apigenin
Celery, parsley, sweet peppers Antioxidants, anti‐cancer
Isoflavones:
Genestein, Daidzein, Glycitein
Soy and other beans Menopausal health, bone health
Source: Darryl Holliday.
c16.indd 348 1/14/2016 12:51:11 PM
CHAPTER 16 • Developing Nutritious Food Products • 349
to preserve food. In recent years, it has been shown to
decrease irritable bowel syndrome symptoms and compete
successfully against unfriendly bacteria.69
While until recently foods and supplements containing pre‐
and probiotics were more common in Japan and European
countries than in the United States, today we are seeing an
influx of products claiming the presence of friendly bacteria.
In fact, Americans’ spending on probiotic foods and supple-
ments exceeded $900 million in 2011.70
Bioactive Ingredients/Phytonutrients
Bioactive compounds are those that, when present in or
added to foods, may have a therapeutic effect in the body.
Phytonutrients are bioactive compounds that originate in
plants. Antioxidants are bioactive because, when absorbed,
they protect the body by preventing lipid oxidation and
breakdown, or by reducing free radicals involved in destruc-
tive oxidative reactions, including those involved in aging.
Another example of bioactivity is the reduction in plasma
cholesterol due to isoflavones (a type of plant hormone). The
inclusion of plant isoflavones in products such as margarine,
salad dressing, and orange juice provides this cholesterol‐
lowering effect in functional foods that are convenient for the
consumer. Documentation of the benefits of adding bioactive
ingredients to foods is difficult, as many appear gradually
over time, making it difficult to prove a direct cause‐and‐
effect relationship. However, antioxidants, which prevent
lipid oxidation in the short term, are proven to reduce the
risk of cardiovascular disease and some types of cancer.71
Phenolics
Phenolics encompass a wide range of phytochemicals (natu-
rally occurring chemical compounds in plants that may be
beneficial for health), ranging from relatively simple phenolic
acids to flavonoids to complex anthocyanins and tannins.
Scientific studies indicate that many phenolics provide sig-
nificant health benefits.72 For example, it is widely accepted
that regular consumption of red wine (which contains the
phenolic compound resveratrol) is associated with the
French Paradox, wherein even though the French diet is rich
in fats, particularly saturated fat, the incidence of heart dis-
ease in France is much lower than in other Western countries
with similar fatty diets. Wine and tea polyphenols possess
antioxidant activity, which may reduce total cholesterol and
lower lipoprotein oxidation concentrations and chronic
inflammation. Catechin, a polyphenolic in tea, is shown to
provide increased antioxidant capacity.73
Anthocyanins are another group of bioactives that may
provide health benefits. These colored compounds are found
in berries, red wine, and purple vegetables.
One measure of the antioxidant potential of a food is its
oxygen radical absorbance capacity (ORAC). This analytical
tool measures the ability of a food or ingredient to remove oxy-
gen radicals in a model system. However, the relationship
between these in vitro (“in glass”) measurements done on a lab
bench and the ultimate health benefit these foods may provide
remains controversial because the antioxidant components
must be absorbed by the body in order to have an effect. That
said, since improved antioxidant status in the body helps pre-
vent chronic diseases, and antioxidants from foods are widely
available, plenty of new opportunities are emerging to deliver
convenient foods with high antioxidant capacity.
Flavonoids
Flavonoids represent a family of over 5000 bioactive com-
pounds derived from fruits and vegetables that deliver high
antioxidant activity. These materials can be extracted, con-
centrated, and used as food ingredients in functional foods or
as nutraceutical supplements. A neutraceutical is different
from a functional food:
A functional food is similar in appearance to, or may actually
be, a conventional food consumed as part of a usual diet that
is demonstrated to have physiological benefits or reduce the
risk of chronic disease beyond basic nutritional functions—
that is, it contains bioactive compounds.
A nutraceutical is a product isolated or purified from foods
that is generally sold in medicinal forms not usually
associated with foods. A nutraceutical is demonstrated to
have a physiological benefit or provide protection against
chronic disease. Examples include dietary supplements and
herbal products.
FIGURE 16.25 Phytonutrients and their
proposed activity.
Phytonutrient Proposed Activity
Anthocyanin Antioxidant. Reduces risk of cancer.
Prevents urinary tract infection.
Beta‐carotene Antioxidant. Reduces risk of some
cancers. Improves lung function.
Carotenoids Antioxidants.
Ellagic acid Reduces risk of some cancers. Lowers
cholesterol levels.
Hesperetin Protects against heart disease.
Liminoids Antioxidants. Protect against heart
disease by lowering cholesterol.
Lutein Maintains healthy vision. Reduces
the risk of cataracts, macular
degeneration, and some cancers.
Lycopene Reduces risk of prostate cancers and
heart disease.
Monterpenes Reduce the risk of some cancers.
Polyphenols Antioxidants. Anti‐inflammatory.
Resveratrol Antioxidant. Reduces the risk some
cancers.
Tannins Reduce risk of heart disease.
Tangeritin Protects against some cancers.
Source: Darryl Holliday.
c16.indd 349 1/14/2016 12:51:11 PM
350 • CULINOLOGY®
Conclusion
Increased focus on nutrition is a growing trend and market-
ing tool in and of itself. As new ideas surrounding nutrition
and health innovations continue to develop, strategies to
capitalize on the consumer’s desire for a healthier diet will
open the door for new and improved food product develop-
ment. Organic foods, gluten‐free foods, and GMO‐free foods
are already experiencing exploding growth, and any company
that can offer foods in any (or all) of these categories will have
a competitive advantage over those that don’t. What does the
future hold? More foods and beverages that promise condi-
tion‐specific benefits, such as stress‐reducing foods, are likely
to have a growing presence in the food industry. Also, brain
foods, bone foods, digestive‐health foods, and appetite‐
curbing foods are gaining momentum. Options for diabetics
will be seen even more frequently. Interest in natural
nutritional ingredients, including hempseed and hemp oil,
antioxidant‐potent spices (for example, cumin, cinnamon,
and turmeric), gut‐friendly pre‐ and probiotics, and high
omega‐3‐foods like flax, will continue to grow.
The development of health‐promoting foods represents
major opportunities for all stages of the food industry. Both
conventional plant breeding and modern transgenic tech-
niques can be used to increase bioactive ingredients, increase
fiber and resistant starch, increase proteins that deliver bio-
activity, and alter lipid structure to produce healthier lipids,
less prone to oxidation and more nutritionally beneficial.
Culinology® professionals can develop high‐quality, great‐
tasting, healthier alternatives to high‐fat, high‐sodium, high‐
sugar foods. As an integrated system, the food industry can
deliver significant improvements to the diet, which can
markedly enhance public health through the reduction of
caloric density and increased levels of health‐promoting
ingredients.
1www.choosemyplate.gov/myplate.
2Centers for Disease Control, “Fruit and Vegetable Consumption Data and
Statistics: Data and Statistics Display,” retrieved on January 21, 2010, from
http://apps.nccd.cdc.gov/5ADaySurveillance/.
3J. Schwartz, and C. Byrd‐Bredbenner. “Portion Distortion: Typical Portion
Sizes Selected by Young Adults.” Journal of the American Dietetic Association
106, no. 9 (2006): 1412–1418.
4Department of Health and Human Services and U.S. Dept. of Agricul-
ture, Dietary Guidelines for Americans 2005, 6th ed. www.healthierus.gov
/dietaryguidelines.
5M. Condrasky, J. H. Ledikwe, J. E. Flood, and B. J. Rolls, “Chefs’ Opinions of
Restaurant Portion Sizes,” Obesity 15 (2007): 2086–2094.
6B. J. Rolls, The Volumetrics Eating Plan: Techniques and Recipes for Feeling
Full on Fewer Calories (New York: HarperCollins, 2005).
7_____, “The Supersizing of America: Portion Size and the Obesity Epidemic,”
Nutrition Today 38 (2003): 42–53.
8B. J. Rolls, The Volumetrics Eating Plan.
9U.S. Department of Health and Human Services and U.S. Department of
Agriculture. Dietary Guidelines for Americans, 2005, 6th ed. (Washington,
DC: U.S. Government Printing Office, 2005).
10www.choosemyplate.gov/myplate.
11Ibid.
12J. C. Rickman, D. M. Barrett, and C. M. Bruhn, “Nutritional Comparison of
Fresh, Frozen and Canned Fruits and Vegetables. Part I. Vitamins C and B
and Phenolic Compounds,” Journal of the Science of Food and Agriculture 87
(2007): 930–944. doi: 10.1002/jsfa.2825
13J. C. Rickman, C. M. Bruhn, and D. M. Barrett, “Nutritional Comparison of
Fresh, Frozen, and Canned Fruits and Vegetables. Part II. Vitamin A and
Carotenoids, Vitamin E, Minerals and Fiber,” Journal of the Science of Food
and Agriculture 87 (2007): 1185–1196. doi: 10.1002/jsfa.2824
14J. Bonke, “Economic Influences on Food Choice—Non‐convenience versus
Convenience Food Consumption,” in H. MacFie and H. L. Meiselman, eds.,
Food Choice, Acceptance and Consumption (2012): 293–318.
15https://www.gnu.org/software/gnutrition/
16J. D. Wright et al., “Trends in Intake of Energy and Macronutrients: United
States, 1997–2000,” MMWR 2204 (53): 80–82.
17Boutrif (1991); Schaafsma (2000).
18Schaafsma (2000).
19A. Chaddha and K. A. Eagle, “Omega‐3 Fatty Acids and Heart Health,”
Circulation 132 (2015): e350–e352.
20A. P. Simopoulos, “The Importance of the Omega‐6/Omega‐3 Fatty Acid
Ratio in Cardiovascular Disease and Other Chronic Diseases,” Experimental
Biology and Medicine 233, no. 6 (2008): 674–688.
21http://www.heart.org/HEARTORG/GettingHealthy/NutritionCenter
/HealthyD ietGoals/Fish‐and‐Omega‐3‐Fatty‐Acids_UC M_303248_
Article.jsp#.
22F. B. Hu, J. E. Manson, and W. C. Willett, “Types of Dietary Fat and Risk of
Coronary Heart Disease: A Critical Review.” Journal of the American College
of Nutrition 61, no. 1 (2001): 5–19; M. Fleith and M. Clandirin, “Dietary
PUFA for Preterm and Term Infants: Review of Clinical Studies,” Critical
Reviews in Food Science and Nutrition 45, no. 3 (2005): 205–229.
23P. French, C. Stanton, F. Lawless, E. G. O’Riordan, F. J. Monahan, P. J. Caffrey,
and A. P. Moloney, “Fatty Acid Composition, Including Conjugated Linoleic
Acid, of Intramuscular Fat from Steers Offered Grazed Grass, Grass Silage,
or Concentrate‐based Diets,” Journal of Animal Science 78, no. 11 (2000):
2849–2855.
24A. Bhattacharya, J. Banu, M. Rahman, J. Causey, and G. Fernandes,
“Biological Effects of Conjugated Linoleic Acids in Health and Disease,” The
Journal of Nutritional Biochemistry 17, no. 12 (2006): 789–810.
25http://www.fda.gov/downloads/Food/IngredientsPackagingLabeling
/GRAS/NoticeInventory/UCM269264.
26L. Tappy and K.‐A. Le, “Metabolic Effects of Fructose and the Worldwide
Increase in Obesity,” Physiological Reviews 90, no. 1 (2010): 23–46.
27L. Brown, B. Rosner, W. Willett, and F. Sacks, “Cholesterol‐lowering Effects
of Dietary Fiber: A Meta‐analysis,” The American Journal of Clinical
Nutrition 69, no. 1 (1999): 30–42.
28J. W. Anderson and K. Ward, “Long‐term Effects of High‐carbohydrate,
High‐fiber Diets on Glucose and Lipid Metabolism: A Preliminary Report
on Patients with Diabetes,” Diabetes Care 1, no. 2 (1978): 77–82.
29M. Roberfroid, “Dietary Fiber, Inulin, and Oligofructose: A Review
Comparing Their Physiological Effects,” Critical Reviews in Food Science
and Nutrition 33, no. 2 (1993): 103–148.
30P. D. Schley and C. J. Field, “The Immune‐enhancing Effects of Dietary
Fibres and Prebiotics,” British Journal of Nutrition 87, Suppl. 2 (2002):
S221–S230.
31C. Coudray, C. Domigne, and Y. Rayssiguier, “Effects of Dietary Fibers on
Magnesium Absorption in Animals and Humans,” The Journal of Nutrition
133, no. 1 (2003): 1–4.
32J. R. Hess, A. M. Birkett, W. Thomas, and J. L. Slavin, “Effects of Short‐chain
Fructooligosaccharides on Satiety Responses in Healthy Men and Women,”
Appetite 56, no. 1 (2011): 128–134.
33M. D. Robertson, J. M. Currie, L. M. Morgan, D. P. Jewell, and K. N. Frayn,
“Prior Short‐term Consumption of Resistant Starch Enhances Postprandial
Insulin Sensitivity in Healthy Subjects,” Diabetologia 46, no. 5 (2003):
659–665.
c16.indd 350 1/14/2016 12:51:11 PM
http://www.heart.org/HEARTORG/GettingHealthy/NutritionCenter/HealthyDietGoals/Fish-and-Omega-3-Fatty-Acids_UCM_303248_Article.jsp#
http://www.fda.gov/downloads/Food/IngredientsPackagingLabeling/GRAS/NoticeInventory/UCM269264
http://apps.nccd.cdc.gov/5ADaySurveillance
http://www.healthierus.gov
http://www.gnu.org/software/gnutrition
CHAPTER 16 • Developing Nutritious Food Products • 351
34A. Nilsson, E. Ostman, T. Preston, and I. Bjorck, “Effects of GI vs Content of
Cereal Fibre of the Evening Meal on Glucose Tolerance at a Subsequent
Standardized Breakfast,” European Journal of Clinical Nutrition 62, no. 6
(2008): 712–720.
35D. Mikulikova and J. Kraic, “Natural Sources of Health‐promoting Starch,”
Journal of Food and Nutrition Research 45, no. 2 (2006): 69–76.
36G. Zhang and B. R. Hamaker, “Review : Cereal Carbohydrates and Colon
Health,” Cereal Chemistry 87, no. 4 (2010): 331–341.
37S. Kajiyama, G. Hasegawa, M. Asano, H. Hosoda, M. Fukui, N. Nakamura, J.
Kitawaki, S. Imai, K. Nakano, M. Ohta, T. Adachi, H. Obayashi, and T.
Yoshikawa, “Supplementation of Hydrogen‐rich Water Improves Lipid and
Glucose Metabolism in Patients with Type 2 Diabetes or Impaired Glucose
Tolerance,” Nutrition Research 28, no. 3 (2008): 137–143.
38E. A. Dennis, A. L. Dengo, D. L. Comber, K. D. Flack, J. Savla, K. P. Davy, and
B. M. Davy, “Water Consumption Increases Weight Loss During a
Hypocaloric Diet Intervention in Middle‐aged and Older Adults,” Obesity
18, no. 2 (2009): 300–307.
39B. M. Davy, E. A. Dennis, A. L. Dengo, K. L. Wilson, and K. P. Davy, “Water
Consumption Reduces Energy Intake at a Breakfast Meal in Obese Older
Adults,” Journal of the American Dietetic Association 108, no. 7 (2008): 1236–1239.
40http://www.reuters.com/article/2011/07/12/us‐campbellsoup‐idUSTRE
76B2V320110712.
41K. G. Dewey and K. H. Brown, “Update on Technical Issues Concerning
Complementary Feeding of Young Children in Developing Countries and
Implications for Intervention Programs,” Food and Nutrition Bulletin 24, no.
1 (2003): 5–28.
42C. Lutter and K. Dewey, “Proposed Nutrient Composition for Fortified
Complementary Foods,” Journal of Nutrition 133, no. 9 (2003): 3011S–3020S.
43http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19940015664 .
44M. Heron and Centers for Disease Control, “Deaths: Leading Causes for
2004,” National Vital Statistics Reports (November 20, 2007), http://www
.cdc.gov/nchs/data/nvsr/nvsr56/nvsr56_05 .
45http://www.cdc.gov/diabetes/pdfs/library/diabetesreportcard2014 .
46http://www.diabetes.org/diabetes‐basics/diagnosis/?loc=db‐slabnav.
47American Diabetes Association (2009), http://www.diabetes.org/home.jsp.
48U.S. News & World Report, “Healthiest Diet” (November 1, 2011) and “Best
Diet Overall” ( June 7, 2011), http://health.usnews.com/best‐diet/best
‐overall‐diets.
49S. Clark et al., “Frequency of U.S. Emergency Department Visits for Food‐
Related Acute Allergic Reactions,” Journal of Allergy and Clinical Immunology
127, no. 3 (2001): 682–683.
50Centers for Disease Control, National Center for Chronic Disease Prevention
and Health Promotion, Division of Adolescent and School Health (October 23,
2008) http://www.cdc.gov/healthyyouth/foodallergies/#2.
51K. Jackson et al., “Trends in Allergic Conditions Among Children: United
States, 1997–2011,” National Center for Health Statistics Data Brief (2013),
retrieved from www.cdc.gov/nchs/data/databriefs/db10.htm.
52S. H. Sicherer, “Food Allergy,” Lancet 360 (2002): 701–710; S. H. Sicherer, A.
Munoz‐Furlong, and H. A. Sampson, “Prevalence of Peanut and Tree Nut
Allergy in the United States Determined by Means of Random Digit Dial
Telephone Survey: A Five‐Year Follow‐Up Study,” Journal of Allergy and
Clinical Immunology 112 (2003):1203–1207; J. Grundy et al., “Rising Prevalence
of Allergy to Peanut in Children: Data from Two Sequen tial Cohorts,” Journal
of Allergy and Clinical Immunology 110 (2002): 784–789.
53S. L. Taylor and J. L. Baumert, “Cross‐Contamination of Foods and
Implications for Food Allergic Patients,” Current Allergy and Asthma Reports
10, no. 4 (2010): 265–270.
54S. L. Hefle, T. J. Furlong, L. Niemann, H. Lemon‐Mule, S. Sicherer, and S. L.
Taylor, “Consumer Attitudes and Risks Associated with Packaged Foods
Having Advisory Labeling Regarding the Presence of Peanuts,” Journal of
Allergy and Clinical Immunology 120, no. 1 (2007): 171–176.
55P. Gupta, M. J. Favus, H. Andrew, and S. Guandalini, “Prevalence of Celiac
Disease in Patients with Osteoporosis in United States,” The American
Journal of Gastroenterology 107 (2012): 1538–1544.
56MarketsandMarkets.com (May 2013).
57Mintel, “Gluten Free Food—US,” September 2013 Report.
58http://www.fda.gov/Food/NewsEvents/ConstituentUpdates/ucm407867
.htm.
597 CFR Part205 http://www.ecfr.gov/cgi‐bin/text‐idx?c=ecfr&sid=3f34f4c22
f9aa8e6d9864cc2683cea02&tpl=/ecfrbrowse/Title07/7cfr205_main_02
.tpl.
60http://www.ams.usda.gov/about‐ams/programs‐offices/national‐organic
‐program.
61http://www.fsis.usda.gov/wps/portal/fsis/topics/food‐safety‐education
/get‐answers/food‐safety‐fact‐sheets/food‐labeling/meat‐and‐poultry
‐labeling‐terms/meat‐and‐poultry‐labeling‐terms/.
62“Backgrounder: Functional Foods,” in Food Insight Media Guide (Washington,
DC: International Food Information Council Foundation, 1998).
63“Position of the American Dietetic Association: Functional Foods,” Journal
of the American Dietetic Association 104 (2004): 814–826.
64Ibid.
65Ibid.
66I. Siró, E. Kápolna, B. Kápolna, and A. Lugasi, “Functional Food. Product
Development, Marketing and Consumer Acceptance—A Review,” Appetite
51, no. 3 (2008): 456–467.
67E. K. Costello, C. L. Lauber, M. Hamady, N. Fierer, J. I. Gordon, and R. Knight,
“Bacterial Community Variation in Human Body Habitats Across Space
and Time,” Science 326, no. 5960 (2009): 1694–1697.
68G. Reid and J. A. Hammond, “Probiotics: Some Evidence of Their Effec-
tiveness,” Canadian Family Physician 51 (2005): 1487–1493.
69J. A. J. Madden and J. O. Hunter, “A Review of the Role of the Gut Microflora
in Irritable Bowel Syndrome and the Effects of Probiotics,” British Journal of
Nutrition 88 (2002): s67–s72; E. M. M. Quigley and B. Flourie, “Probiotics
and Irritable Bowel Syndrome: A Rationale for Their Use and an Assessment
of the Evidence to Date,” Neurogastroenterology and Motility 19, no. 3 (2006):
166–172.
70“Probiotics Lead the Digestive Market,” Natural Products Insider (March 11,
2013).
71M. G. L. Hertog et al., “Dietary Antioxidant Flavonoids and Risk of Coronary
Heart Disease: The Zutphen Elderly Study,” Lancet 342, no. 8878 (1993):
1007–1011; D. L. Tribble, “Antioxidant Consumption and Risk of Coronary
Heart Disease: Emphasis on Vitamin C, Vitamin E, and Beta‐Carotene: A
Statement for Healthcare Professionals from the American Heart Asso-
ciation,” Circulation 99 (1999): 591–595.
72R. H. Liu, “Health Benefits of Fruit and Vegetables Are from Additive and
Synergistic Combinations of Phytochemicals,” American Journal of Clinical
Nutrition 78, no. 3 (2003): 517S–520S.
73W. E. Bronner and G. R. Beecher, “Method for Determining the Content of
Catechins in Tea Infusions by High‐Performance Liquid Chromatography,”
Journal of Chromatography 1–2, no. 805 (1998): 137–142; Z. Y. Chen et al.,
“Degradation of Green Tea Catechins in Tea Drinks,” Journal of Agricultural
and Food Chemistry 49, no. 1 (2001): 477–482; T. T. C. Yang and M. L. W. Koo,
“Chinese Green Tea Lowers Cholesterol Level Through an Increase in Fecal
Lipid Excretion,” Life Sciences 5, no. 66 (1999): 4111–4123.
c16.indd 351 1/14/2016 12:51:11 PM
http://www.reuters.com/article/2011/07/12/us-campbellsoup-idUSTRE76B2V320110712
http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19940015664
http://www.cdc.gov/nchs/data/nvsr/nvsr56/nvsr56_05
http://health.usnews.com/best-diet/best-overall-diets
http://www.fda.gov/Food/NewsEvents/ConstituentUpdates/ucm407867.htm
http://www.ecfr.gov/cgi-bin/text-idx?c=ecfr&sid=3f34f4c22f9aa8e6d9864cc2683cea02&tpl=/ecfrbrowse/Title07/7cfr205_main_02.tpl
http://www.ams.usda.gov/about-ams/programs-offices/national-organic-program
http://www.fsis.usda.gov/wps/portal/fsis/topics/food-safety-education/get-answers/food-safety-fact-sheets/food-labeling/meat-and-poultry-labeling-terms/meat-and-poultry-labeling-terms/
http://www.cdc.gov/diabetes/pdfs/library/diabetesreportcard2014
http://www.diabetes.org/diabetes%E2%80%90basics/diagnosis/?loc=db%E2%80%90slabnav
http://www.diabetes.org/home.jsp
http://www.cdc.gov/healthyyouth/foodallergies/#2
http://www.cdc.gov/nchs/data/databriefs/db10.htm
352 • CULINOLOGY®
17
Sensory Evaluation
Introduction
Sensory evaluation, also called organoleptic analysis , is a
multidisciplinary science used to understand human per-
ception and response to the sensory characteristics of food.
Its foundation comprises empirical and theoretical contri-
butions from many fields, including the psychology of human
perception, sensory physiology, social science, anthropol-
ogy, marketing, and food science as well as chemistry and
statistics. 1
Research chefs are increasingly integrated into the prod-
uct development process. Although they can contribute at
many stages, research chefs are usually involved early on, par-
ticularly during the concept and prototype development
phases. 2 Many chefs develop what is called the culinary gold
standard , namely, the product that serves as the benchmark
for subsequent commercialization (see Chapter 19 ). Once the
gold standard is established, the research and development
(R&D) team develops the production model and runs scale‐
up and pilot plant tests. 3
Collaboration between sensory evaluation, Culinology®,
product development, and marketing research is important
and necessary in order to improve the likelihood of market-
place success. Sensory evaluation techniques are commonly
used throughout the product development process, from
inception to launch, in order to translate consumer insights
into product design as well as processing specifications.
Business decisions and product development direction often
rely on sensory feedback to reduce the risk of failing to meet
consumer needs and expectations.
The Product
Development Chef
and Sensory Evaluation
A chef developing foods for mass production, where the end
users can number in the millions, must depart from typical
chef thinking. Traditional restaurant chefs are trained to be
the primary decision makers about recipe development,
menu design, cooking and plating style—virtually everything
related to preparing and presenting the food to their (hope-
fully) adoring public. Chefs figuratively put themselves on
every plate that leaves the kitchen; the more idiosyncratic,
the more personalized they make the food, the more com-
petitive they are in a highly competitive market. If the chef
cannot generate a following, the restaurant will fail. All of the
pressure for the restaurant to succeed is on the chef ’s shoul-
ders. Yet, in a mass manufacturing setting, commercial reality
must intervene. When developing products for the main-
stream marketplace, where enormous amounts of money are
at risk, the opinion of a single individual, even an experienced
culinary professional, is only marginally important; chefs
need the input of other product development professionals
and actual consumers to be successful in this arena.
Chefs are generally not good at giving up control. After all,
the word chef comes from the French for “chief.” The reality is
that in most kitchens the chef ’ s palate is the sole source of
direction. By comparison, in commercial product develop-
ment all parties, chef included, must be ready to relinquish
that control and to accept the direction provided by the
Lead Author: Witoon Prinyawiwatkul, Ph.D. , Professor, School of Nutrition and Food Sciences ,
Louisiana State University Agricultural Center
Manager of Product Innovation , Smithfield
Director, Sensory and Consumer Insights , Nestlé
Director of Culinary Development , Red Lobster
Chairman, iNovum LLC, Chairman , Mind Genomics Associates
Robert Delaney ,
M. Michele Foley ,
Dustin Hilinski ,
Howard R. Moskowitz, Ph.D. ,
uva u
Sc
at
ood Sciences ,
c17.indd 352 1/22/2016 1:19:14 PM
CHAPTER 17 • Sensory Evaluation • 353
consumer. Product development (PD) chefs must accept
continuous (not always positive) feedback and become
accustomed to participating as an equal member in a cross‐
functional team, where they may not be the leader. The chef ’s
input is certainly valued, but it is only one part of the suc-
cessful product development equation. The company must
deliver the best possible product to consumers at reasonable
cost, safely, and with good stability. The consumer is the glue
in the puzzle that combines culinary art with food science.
The marketplace has a lot to say. The days of launching a
product based on the opinions of a few are over; the market-
place is just too competitive. The cost of launching new items is
prohibitive. Retailers routinely challenge what goes on their
shelves; slotting fees in the millions of dollars are commonly
imposed on food manufacturers to put a new product onto an
already overstocked grocery shelf in a large chain supermarket.
To remain on the shelf and to see a return on their investment,
food manufacturers must listen to the voice of the consumers.
Most consumer feedback is based on personal preference
developed through a lifetime of family influences and other
life experiences. For product developers, it is an enormous
source of information that allows them to better design or
improve products for specific consumer populations.
In general, chefs who are new to the corporate world are at
a bit of a disadvantage. Restaurant chefs lack the experience,
knowledge, and understanding of the benefits of consumer
testing. Their only knowledge of sensory analysis is what is
technically called a subjective affective or preference test; if
something on their menu doesn’t sell—that is, is “not
preferred”—it is dropped. On the other hand, in the food man-
ufacturing world, the chef is provided with an enormous vari-
ety of data from a battery of sensory tests; this can be mined
for valuable information to use in developing better, more
marketable products. But in order to be heard, chefs must
speak the same language as the other people on the PD team.
Knowledge of common sensory terminology is essential.
In the past, product developers (scientists and technolo-
gists) rarely learned the culinary arts, which is to some degree
an emotional process rather than a science. Food excites
emotional responses in all of us, and chefs tend to cook with
emotion. But beyond art or science is business. The product
must be developed in such a way that it can be manufactured
consistently and economically and distributed widely at very
high volumes with an extended shelf life, in a safe, whole-
some, and affordable way. So art and emotion must merge
with commercial reality. Understanding and integrating
these sometimes competing objectives goes a long way
toward improving the working relationship between art and
business. With Culinology®, the barriers are finally being bro-
ken, with more food scientists exploring culinary arts and
more chefs studying food science with the common goal of
giving consumers the best possible foods while maximizing
profit for their companies.
The explosion of information in the media has made con-
sumers better versed in food than ever before. They have
learned to speak some of the chef ’s language and are more
attentive to details such as texture, flavor intensity, ethnic and
regional authenticity, and cooking methods. And the consumer
is, without a doubt, the most powerful member of the product
development team. Sensory evaluation, working with consum-
ers, provides direction for both the chef and the food scientist,
who can incorporate the consumer’s responses into the product
development process. Even after a product is finalized, sensory
evaluation continues to play an essential role in monitoring
scale‐up, process changes, cost reductions, product improve-
ments, and shelf‐life testing. With the ever‐increasing costs of
launching new products, the enormous number of new product
failures, and rapidly multiplying competition, sensory eva l-
uation is clearly critical to success in today’s food industry.
Technical Aspects
and Applications of
Sensory Evaluation
Products attain market success when they meet a consumer
need at an appropriate price, when their benefits are well com-
municated to the target consumers, and when they deliver to
the expectations generated by their positioning.4 Consumer
product research is a tool used to achieve all these factors by
measuring preference, liking, and attitudes of consumers
toward a product as a whole, toward specific characteristics,
product concepts, and even services wrapped around prod-
ucts. Consumer responses are important in new product
development, product improvement, product maintenance,
new product potential or category appraisal, and product
claim substantiation. Most successful new product efforts
start and end with the consumer.5 As such, the use of con-
sumer product research has become more common, and the
results are more widely used to aid the business decision‐mak-
ing process. Nowadays, whether a company develops new
products, copies competitor products, or attempts to enter
new market segments, input from sensory evaluation is vital.
The Five “In” Steps of the
Product Development
Pathway
The following five innovation steps are used throughout the
product development cycle: insight, inspiration, interpreta-
tion, introduction, and inspection.
1. Insight—The product must solve an unmet consumer need
or problem. With constantly changing marketplace and
consumer trends, it is increasingly difficult to predict con-
sumer responses. Uncovering consumer needs is critical in
the early stages of the product development process.
During the insight stage, researchers use exploratory quali-
tative methods to help identify needs and quantitative
methods to estimate the size of the opportunity.
c17.indd 353 1/14/2016 7:44:45 AM
354 • CULINOLOGY®
2. Inspiration—To meet a need identified in the insight stage,
ideas are generated through ideation sessions and other
creative thought–provoking activities. These might be
written concepts developed by a cross‐functional product
development team or culinary prototypes created by PD
chefs to represent these concepts. Typically, ideas are
screened based on overall appeal (as measured by accept-
ance or purchase intent data) and uniqueness, both quali-
tatively and quantitatively. It is important for the product
development team leader (usually the most experienced
member of the team) to remain involved throughout this
process to ensure the prototypes are realistic and can be
produced commercially.
3. Interpretation—Based on concepts and culinary prototypes,
product developers formulate commercialized products that
use commercial ingredients and are designed to be man-
ufactured on the company’s equipment. At this stage, more
detailed estimates are made about the potential size of the
business. Understanding how the new product will affect the
current business, whether it will be used by consumers in
addition to or as a replacement for a current product, is
important at this stage. Sensory methods are used with both
FIGURE 17.1 Some common questions that
require sensory‐related answers.
1. Are the two products different? If so, in what way do
they differ?
2. Is our product similar to the competitor’s?
3. Which of the two products is sweeter? Which one is
sweetest among these five?
4. How can I evaluate the intensity of sweetness of this
product?
5. Which product is more preferred by consumers?
6. Is the improved or reformulated product better than
our current or competitors’?
7. Will the taste and texture change if we use cheaper
ingredients?
8. How does the use of a new twin‐screw extruder impact
overall sensory quality?
9. How consistent in quality are all products from different
batches and locations?
10. What was the cause of the product disliking and the
negative purchase intent?
11. What would be the optimal salt and sugar
concentration for this product?
12. How can I mimic the flavor and texture of this product?
13. Based on sensory liking, can we predict consumers’
willingness to purchase?
14. How can I mask the bitterness but enhance the saltiness
of this product?
15. How do consumers respond to the product
performance or the baking instruction?
16. What do consumers think about this new product
concept?
17. How do consumers use the products? How do they fit
into consumers’ lives?
Source: Witoon Prinyawiwatkul, Ph.D., Robert Delaney, M. Michele
Foley, Dustin Hilinski, and Howard R. Moskowitz, Ph.D.
external consumers and internal descriptive panels. Estimates
of business impact are made from consumer responses.
Comparative descriptive sensory evaluation is used to ensure
that commercialized product formulations maintain close
similarity to the culinary gold standards.
4. Introduction—Commercial product formulas with specific
processing instructions are transferred from product
developers to manufacturing for scale‐up testing on actual
manufacturing equipment. At this stage it is critical that
the production samples closely represent the original gold
standard target. Measuring the delivery of similarity to tar-
get is a key sensory activity. Analytical sensory tools (dis-
crimination and descriptive analysis) are the primary
methods at this stage. Discrimination methods are sorting
techniques used to assess similarity to the target, while
descriptive analysis helps researchers understand how the
production samples compare to the target on key sensory
attributes.
5. Inspection—Once the product is in the market, sensory
methods are used to monitor quality. These can include
quantitative methods similar to those used in the introduc-
tion stage. Monitoring product performance against the
competition may combine both consumer and descriptive
analysis in which responses from consumers provide
insights into whether the differences in sensory attributes
measured by descriptive analysis are meaningful or relevant
(is it a difference that really makes a difference?). Continued
performance against a target or gold standard typically is
assessed at this stage to measure shelf stability over time.
Sensory Methods
Methods are often categorized by type—qualitative (inter-
views and observations) or quantitative (measures or rat-
ings)—or by how responses are collected—behavioral
(observational), attitudinal (surveys), and analytical (using
trained or expert panelists). Behavioral approaches involve
observing or gathering self‐reports of consumer actions, usu-
ally close to the point of consuming the product, so they repre-
sent actual behavior. Observing, documenting, and then
statistically analyzing what people do rather than asking what
they do provides more accurate information, as people often
tell you what they think you want to hear or are too embar-
rassed to reveal what they actually do. Attitudinal approaches
involve collecting responses such as consumer opinions, feel-
ings, and reactions to stimuli (sensory, psychological, intellec-
tual, and so on), recalled behavior from the past, or expected
action in the future. Responses and ratings to sensory stimuli
provided by experienced or trained respondents or panelists
are classified as analytical methods.
Qualitative Consumer
Research
Qualitative consumer research is an important tool by
which sensory analysts elicit valuable insights into attitudes,
behaviors, habits, emotions, and motivations, based on either
c17.indd 354 1/14/2016 7:44:45 AM
CHAPTER 17 • Sensory Evaluation • 355
observing or directly interviewing consumers. Some types of
product evaluation at the bench are also qualitative (bench
screenings, product cuttings, product reviews). Qualitative
consumer research can be conducted before quantitative
research to generate hypotheses or establish criteria for
data collection (for example, questionnaire development).
Qualitative research may reveal which attributes consumers
believe are important to the products, how they might relate
to “liking or preference,” and how they understand descrip-
tive terms (such as crunchiness or crispness). Thus, informa-
tion from qualitative consumer research may help identify
product opportunities and generate new product concepts.
Although these methods are straightforward and the findings
are typically credible, results are usually further validated in a
larger quantitative test. Commonly used qualitative methods
include focus group interviews, focus panels, one‐on‐one or
in‐depth interviews (IDI), and ethnography. Brief descrip-
tions of common qualitative consumer research methods are
given below.
• Focus group interview—A carefully designed session used
to understand consumers’ perceptions or reactions
toward products, product concepts, or services in a per-
missive, friendly, relaxing, comfortable, and nonthreat-
ening environment. Led by a skilled moderator, each
session usually lasts up to three hours. Standard practice
calls for several separate sessions with different groups
of participating consumers. Each group comprises 9 to
12 participants who represent the target consumers of
the products of interest. Proceedings are often video-
taped to enable researchers to analyze verbal and non-
verbal (body) cues to uncover hidden meaning (if any) in
the responses.
• Focus panel—A variant of the focus group interview where
the same group of consumers participates two or three
FIGURE 17.2 General types of methods used in
product design and development.
Types Behavioral Attitudinal Analytical
Qualitative • Ethno g-
raphy
• Focus
groups
• In‐depth
interviews
• Bench
screening of
prototypes
• Product
reviews
Quantitative • Purchase
or con-
sumption
diaries
• Conjoint
studies
• Category
appraisals
• Variety of
product
testing
methods
(preference,
acceptance)
• Discrimina-
tion testing
• Descriptive
analysis
Source: Witoon Prinyawiwatkul, Ph.D., Robert Delaney, M. Michele
Foley, Dustin Hilinski, and Howard R. Moskowitz, Ph.D.
times. The panel is created to initially discuss the product
of interest; the group is sent home to use the product and
then brought back to discuss its experiences.6
• In‐depth interview—A one‐on‐one interview conducted to
understand attitudes and behaviors in more detail. Such
interviews may be necessary when the issue is sensitive,
highly personal, or emotionally charged, such as weight‐
loss diets or deodorants.7 Laddering is a technique often
used during in‐depth interviews to uncover the subcon-
scious attraction of a product or even an entire category.
Consumers are attracted to specific product or brand
attributes (for example, all‐natural, no preservatives, all
organic ingredients), but they may not be aware of it.
Laddering is a practical means to investigate how product
attributes are linked to functional or emotional benefits. It
involves a series of directed probes, typified by the ques-
tion “Why is this important to you?”8
• Ethnography—Observation is a powerful research tech-
nique used to gather information on who consumers
really are, how and why products are purchased and
used, what they like or dislike, and their demands and
needs.9 Observation can be passive or active. An ethnog-
rapher shadows respondents in their own homes, work-
places, restaurants, at points of purchase in grocery
stores, and so on to observe and record real behaviors
associated with the product, product preparation, or
usage.10
Quantitative Consumer
Research
Quantitative consumer research is conducted with a larger
group of representative consumers (100 to 500) from the tar-
geted segment or population of the market, often divided
over three or four representative cities.11 This type of research
determines overall preference or acceptance of a product (or
line of products) or of specific sensory attributes (color,
aroma, flavor, texture, and so on). In addition, combining
descriptive analysis results with acceptance results helps
identify the specific sensory attributes associated with prod-
uct differences as well as those that drive performance (pref-
erence, rating of liking, purchase intent).12
These tests can also be used to identify and quantify con-
sumer segments and to compare the new or improved prod-
uct against the competitive set: those products and services
already in the market that will compete with it for the tar-
geted consumer’s pocketbook. In addition, at the end of a
project, these tests can be used to confirm the product is
ready to be commercialized. Smaller‐scale studies (50 to 150
respondents) provide less costly research guidance for prod-
uct development teams in the optimization stage.
Preference Tests
Whether the researcher selects preference tests or accept-
ance tests depends on the research objective.13 As a forced‐
choice method, the preference test forces consumers to
c17.indd 355 1/14/2016 7:44:45 AM
356 • CULINOLOGY®
compare one product against another or several others. By
itself, a preference test does not measure degree of liking (or
disliking) of the product, nor does it indicate a degree of dif-
ference. A common misconception is that two products
equally preferred are equally liked or that they are the same.
This can lead to wrong business decisions. Preference tests
only give direction and do not indicate true magnitude of
differences.
Preference tests fall into four distinct types: paired prefer-
ence, rank preference, multiple paired preferences (all pairs
compared), and selected paired preferences (selected pairs
compared).14 The paired preference (“Which of the two sam-
ples do you prefer?”) and rank preference when there are
more than two samples (“Rank the samples from most to
least preferred”) are most commonly used.
Consumer Acceptance Tests
“Acceptance” may be defined as an experience characterized
by a positive attitude (“I like it”) or actual use (“I will pur-
chase or eat it”).15 Consumer acceptance tests measure
overall product acceptance and acceptability of individual
attributes (appearance, color, flavor, texture, and so on).
Many types of scales measure acceptance. The most com-
mon is the 9‐point hedonic scale (hedonic is another word
for liking or pleasure). It has been widely studied for its appli-
cability, validity, and reliability. The nine points of the
hedonic scale are:
1 = dislike extremely
2 = dislike very much
3 = dislike moderately
4 = dislike slightly
5 = neither like nor dislike
6 = like slightly
7 = like moderately
8 = like very much
9 = like extremely
For children and illiterate subjects, other types of scales
are used that modify the labels to smiley faces or simpler lan-
guage.16 The 3‐, 5‐, and 7‐point smiley‐face scales were
reported to be appropriate for 3‐ to 5‐year‐old children.17
The second type of acceptance test is purchase intent.
Typically, hedonic scores and purchase intent ratings are
highly correlated in product tests; in general, a product that
receives a high hedonic score also receives a high purchase
intent score. Differences between hedonic and purchase
intent scores are found when additional information is
provided about the product, such as brand, price, or nutri-
tional information. Both measures (acceptance and purchase
intent) should be used when the test is not a blind taste test
and additional information is provided. Typically this occurs
near the end of the product development phase and before
commercialization to confirm the product meets consumers’
expectations, fits the concept or the brand, and is something
they would buy if it were available where they shop. The pur-
chase intent scale is typically a 5‐point scale:
1 = definitely would not buy
2 = probably would not buy
3 = might or might not buy
4 = probably would buy
5 = definitely would buy
The marketer wants a product that generates a high “top‐
two box” score—that is, most of the responses come back as
“definitely” or “probably would buy.”
Often, acceptability measurement alone does not provide
enough information to develop a product or to refine a proto-
type. The reasons for liking or disliking could be identified
using open‐ended questions or by additional questions about
intensity of critical sensory attributes. One method is the so‐
called just‐about‐right scale ( JAR), often incorporated into
quantitative consumer testing to measure the intensity of
specific sensory attributes with respect to the most preferred
level. The JAR scale is commonly labeled with five points:
much too strong, too strong, JAR, too weak, much too weak.
The combination of hedonic and JAR ratings provides direc-
tion for product reformulation, refinement, or optimization.
Choosing appropriate consumers is of utmost importance
for consumer acceptance tests. Screening criteria to deter-
mine who is an appropriate participant may be based on
demographic, socioeconomic, or psychographic (lifestyle)
criteria. Depending on the business priority and purpose of
the research, participating subjects may be recruited from
various sources. The use of employees for conducting
preliminary tests, to screen samples before the actual (much
more expensive and laborious) fieldwork, is common.
Acceptance tests with employees can also be a resource for
product maintenance purposes—for example, to establish
when a difference in quality becomes evident (product aging,
defect testing).18 For new product development and product
optimization, in‐house or employee consumers should not
be used exclusively because they may not represent the ulti-
mate consumers.
Consumers for product research are selected based on
current behaviors (actual purchase) or attitudes (interest in
purchase). They should be screened for potential bias based
FIGURE 17.3 Smiley‐face scale used with children.
Source: Witoon Prinyawiwatkul, Ph.D., Robert Delaney, M. Michele Foley, Dustin Hilinski, and Howard R. Moskowitz, Ph.D.
c17.indd 356 1/14/2016 7:44:45 AM
CHAPTER 17 • Sensory Evaluation • 357
on employment, knowledge of the business, or simple dislike
of the food (or brand) being tested. Before the actual taste
test, participants should complete a consent form to ensure
they are not allergic to the food and materials they will
be testing.
Measuring Consumer Emotion
about Foods
Emotion, either pleasant or unpleasant, plays an important role
throughout the span of human lives. Emotions evoked by prod-
ucts mainly enhance the pleasure (maybe unpleasantness) of
buying, owning, using, and repeating using them. Nowadays, in a
highly competitive marketplace, it is often argued that emotion
evoked by products is becoming more and more important for
product differentiation, as many products are now often similar
in their characteristics, packaging, and price. In some purchase
decisions, emotional response, rather than sensory liking or
price, may even be the decisive factor.
Consumer emotions evoked by products and product
brands may positively or negatively affect product liking, pur-
chase decision, food choices, desire to eat, and pleasure (and
pride—look how smart I am!) of buying, owning, and using
them. Knowledge of how emotions are evoked enhances
understanding of what makes people enjoy interacting with a
particular food, food name, or product concept. However,
determining how consumers respond emotionally to food
products and what aspects (such as food names, product
design, benefit, and packaging) trigger emotional reactions
can be quite challenging.
Measurement of acceptability is not a sufficient benchmark
for product development and testing. Recently, the EsSense
Profile® was developed to measure mood and emotion in a
product development setup; it yields information not normally
captured by measuring acceptability.19 The EsSense Profile®
provides a detailed list of 39 emotion attributes (mainly posi-
tive ones) that consumers associate with test products.
Consumer emotion and methods for measuring emotions
associated with foods in consumer testing are summarized
in several studies.20 The relationships between food, eating
behavior, and consumer emotion are examined as well.21 For
example, chocolates (milk and dark) evoked emotions such as
active, affectionate, energetic, happy, loving, and satisfied.22
Sensory Discrimination Tests
Sensory discrimination tests23 are the most basic tests in
sensory science, and probably have the longest history.24
Discrimination tests are generally used to determine whether
or not overall or specific differences exist between two poten-
tially confusable samples. Panelists are typically employees
screened for their sensory acuity. Test methods are classified
into two groups:
1. Overall difference tests—The basic question is “Does a dif-
ference exist between samples?” without specifying an
attribute and with or without a reference for comparison.
2. Attribute difference tests—This type of test specifies attrib-
utes (such as sweetness). Depending on the test, panelists
are asked to determine which sample has more or less
intensity of the specified attribute.
FIGURE 17.4 Commonly used discrimination test methods.
Test Methods Sample Presentation Panelist Task
Suggested
No. of Panelists
2AFC (Alternative
Forced Choice)
2 samples served from 2 possible
combinations (AB or BA)
Indicate which sample exhibits
more or less of a specific
attribute (e.g., sweetness).
A multiple of 2;
≥30 subjects; as few
as 16 subjects
Simple Paired
Comparison or
Same‐Difference
test
2 samples served in sets of 2 from
4 possible combinations:
unmatched (AB or BA)
matched (AA or BB)
Indicate whether the two
samples are same or different.
A multiple of 4;
≥32 subjects; as few
as 12–16 subjects
Triangle 3 samples (2 identical and 1 odd)
served from 6 possible
combinations (ABB, BAA,
AAB, BBA, ABA, and BAB)
Either pick the odd sample or
pick the two identical samples
(must guess when the odd
sample is not detectable).
A multiple of 6;
24–60 subjects
Duo‐Trio 3 samples (identified reference
and 2 blind) served from 2
combinations (Ref‐AB and Ref‐BA)
Indicate which of the 2
unknown samples matches
the reference.
A multiple of 2;
≥32 subjects; as few
as 12–16 subjects
Difference from
Control
First, serve an identified control
sample. Then serve at least
2 unknown samples; one is the
unknown control (placebo); the
rest are the unknown test samples.
Rate the size of the difference
(overall or by attribute)
between each unknown
sample and the identified
control.
30–50 subjects
Source: Witoon Prinyawiwatkul, Ph.D., Robert Delaney, M. Michele Foley, Dustin Hilinski, and Howard R. Moskowitz, Ph.D.
c17.indd 357 1/14/2016 7:44:45 AM
358 • CULINOLOGY®
Sensory Descriptive Analysis
Sensory descriptive analysis is the most sophisticated sensory
method by which the attributes of a product are identified,
characterized, and quantified, using an expert panel specifi-
cally trained for such purpose. Descriptive analysis is primarily
used for product development, quality control, shelf life, prod-
uct claim substantiation, and category appraisal. The compo-
nents of commonly used methods include both qualitative
(lexicon or descriptor development) and quantitative (inten-
sity rating) aspects.
The source for panelists varies with availability and demand.
Training and panel participation require a significant time com-
mitment. Often it is difficult for employees to invest the time
required to participate actively in training and ongoing panel
sessions. Many sensory groups hire consumers on a part‐time
basis to participate as descriptive panelists. This allows the pan-
elists to focus completely on the analysis of products without the
distraction of another job. During the training phase of most
descriptive analysis methods, the panel develops a lexicon or list
of descriptors for the product or product category of interest. To
be effective, descriptors should help describe differences in the
sample set and be unique and singular (“fried” or “beefy”), not
complex or abstract (like “fresh”). To help communicate the
attribute and ensure it is used consistently over time, each
descriptor should have a written definition and an identifiable
taste or smell reference standard that is easy to obtain.25
Excellent reviews of sensory descriptive analysis methods
can be found in a number of publications owing to the great
popularity of descriptive analysis since the 1940s.26 Compila-
tion of applications of descriptive sensory analysis in dairy,
meat, alcoholic beverages, and others can be found in
Descriptive Sensory Analysis in Practice.27 A comprehensive
list of descriptors for finished products, along with defini-
tions, references, and reference preparation can be found in
Aroma and Flavor Lexicon for Sensory Evaluation: Terms,
Definitions, References, and Examples.28
Basic Sensory Attributes
Humans possess five primary senses for perceiving stimuli:
sight, hearing, touch, smell, and taste. Other human senses
include temperature sensation (heat and cold), pain, visceral
hunger, thirst, and fatigue.29 The “sensory receptor devices”
(that is, eyes, ears, skin, tongue, and nose) respond to a particu-
lar range of environmental stimuli and transmit corresponding
information to the brain via the central nervous system.
With respect to food, taste and smell receptors respond to
specific chemical stimuli, and sight, hearing, touch, and tem-
perature receptors respond to physical stimuli.
Humans tend to perceive food attributes in two consump-
tion stages: approach or distal stage (based on appearance and
odor or aroma) and consumption or proximal stage (based on
stimuli including consistency, texture, and flavor, which is a
combination of taste, aromatics, and chemical feelings).
However, these attributes inevitably overlap, as people tend to
perceive the food they are eating in its entirety rather than its
component parts.30 Basic sensory attributes of foods include:
• Appearance—This includes traits such as color, shape,
and size as well as more complex attributes such as sur-
face texture, structural uniformity, and visual flavor.31
Visual flavor is a gestalt term. The overall appearance of a
product may make consumers think that the product has
a specific flavor. For example, a jelly with green color may
have lime or green apple flavor. A darker red color juice
may be sweeter than one with a lighter red color.
Appearance is the most important sensory characteris-
tic in determining initial consumer acceptance of prod-
ucts, and it is often the only attribute on which people
can base a decision to purchase or consume, particularly
if the food is hidden inside a package.32
• Odor or aroma—Aroma is the odor of a food produced
by volatilized chemical constituents that originate
externally (orthonasally, or through the nose), often
when the food is heated or cooked, and internally (retro-
nasally) from food in the mouth while it is being con-
sumed. Retronasal (flavor‐by‐mouth) odor is perceived
when a volatile stimulus originating in the mouth passes
the back of the nasopharyngeal passage into the olfac-
tory receptors located in the roof of the mouth.
• Texture—This complex term covers viscosity (describ-
ing homogeneous liquids), consistency (describing non‐
homogeneous liquids and semisolids), and two textural
subcomponents:
1. Mechanical properties (for example, hardness,
springiness) perceived by the kinesthetic nerves
that monitor the position and movements of mus-
cles, bones, and joints
2. Tactile or skinfeel properties (for example, moist-
ness, fibrousness) perceived by the nerves in the
skin of the hands, lips, or tongue
• Flavor—The sum total of the sensory impressions (taste,
odor, and chemical feeling) of a product is perceived via
the chemical senses in the mouth. It is one of the most
important sensory characteristics that influence the
acceptability of foods.33 Humans experience five basic
tastes (salty, sweet, sour, bitter and “umami”—a taste sen-
sation that is meaty or savory and is produced by several
amino acids and nucleotides, generally referred to as “glu-
tamates”). Some sensory authorities believe there may be
other taste reactions, namely: alkaline, metallic, calcium‐y
(the bitterness and slight sourness of vegetables such as
collard greens, kale, bok choy, and bitter melon), and fat
taste “oleoqustus,” which is an unpleasant bitter and sour
flavor. Chemical irritants such as horseradish, mustard,
ginger, onion, garlic, and chiles stimulate the trigeminal
nerve ends, causing perception of pungency and burn or
heat in the eyes, nose, and mouth. Another trigeminal
effect is the fizzy tingle of carbon dioxide in soda.
• Sound—Produced during the first bite and subsequent chew-
ing (mastication) of foods. Differences in pitch, loudness, and
persistence of sounds (crispness, crunchiness, brittleness,
squeakiness) may be used as indices for product quality such
as freshness or staleness, maturity or ripening stages.34
c17.indd 358 1/14/2016 7:44:45 AM
CHAPTER 17 • Sensory Evaluation • 359
Because sensory evaluation is a science dealing with
humans as an active measuring tool, knowledge of anatomy,
physiology, and functions of the human senses is indispensa-
ble to avoiding misinterpretation and incorrect conclusions.
For a brief review of this material, particularly taste, smell,
and trigeminal effects, and for useful references, see Sensory
Evaluation of Food: Principles and Practices.35
Minimizing Errors in Sensory
Measurements
“Error” is not just synonymous with “mistake” but may also
include all sorts of extraneous influences.36 The physical and
psychological condition of the panelist and the influence of the
testing environment can affect sensory results. Extraneous
influences must be minimized to ensure the selected sensory
tests are measured correctly.
Three main factors negatively affect sensory results: physi-
ological, psychological, and physical errors. A person’s ability
to perceive or sense the attributes is influenced by physiologi-
cal factors (mostly related to genetic makeup) and physical
factors (mostly related to health). In contrast, psychological
factors may affect how the person rates the attributes. For
example, having a cold affects a person’s ability to smell (physi-
cal); a person who thinks he recognizes a sample as being a
brand he doesn’t like rates that sample lower in acceptance
(psychological).
Three types of controls (test room, product, and panel)
must be maintained in order to minimize bias and maximize
sensitivity of the selected sensory methods and panelists’
performance.37
1. Test room controls—In addition to having a proper test
room designed specifically for sensory testing (preferably
with several individual tasting booths), the setup, lighting,
noise, air circulation, temperature, and relative humidity
must be controlled in order to minimize bias and maxi-
mize sensitivity of panelists.
2. Product controls—These involve sample preparation and
presentation. For sample preparation, all supplies, utensils,
and equipment must be food‐grade or for food use only.
Care must be taken to ensure that odors from, for example,
plastic utensils and containers, do not change the aroma
and flavor characteristics of the test products. The sample
preparation methods (for example, cooking time and tem-
perature, thawing, and mixing time) should be appropriate
to the product and kept constant throughout the sensory
testing period. The samples should not change in sensory
quality during holding throughout multiple sessions of
sensory tests. If samples tend to change over time, then
multiple fresh batches of the sample must be prepared
throughout the test to assure consistency. In terms of sam-
ple presentation, the number of samples, serving size, serv-
ing pattern, coding, and palate cleansing are of major
FIGURE 17.5 Common bias effects and test design solutions.
Effect Description Solution
Stimulus If subjects know or presume to know the identity of a
sample, then they make assumptions about what it
should taste, smell, or look like based on their
expectations for this sample.
Use naive or unpracticed consumers to
measure liking, use plain serving containers
and random sample identification, and
carefully hide clues to sample identity.
Context Ratings for identical stimuli in different contexts shift.
The set of samples evaluated sets the frame of reference
for judgments. An item may be perceived differently due
to the direct influence of one sample on another that is
nearby in time or space.
Consider the total set of samples and each
sample’s importance to the primary test
objective. Always include a sample in
the test that you know something about
(control).
Order The first sample tasted sets expectations. If the same
sample is presented multiple times, average liking scores
will decrease with each presentation. “First Position
Effect”: the first sample is rated higher.
Alternate the order of presentation of
samples.
Contrast Perceptions are strongly influenced by what was
experienced most recently. Each sample influences
opinions of the next sample. Probably most pronounced
when sensations are extreme.
Alternate the order of presentation of
samples.
Adaptation Subjects experience a decrease in responsiveness
under conditions of constant stimulation. May be at the
level of sensation or conscious perception.
Allow time between samples to cleanse the
palate.
Halo One very positive or negative aspect of a person or
product can influence judgments on other unrelated
characteristics.
Check to be certain that samples do
not differ in some unintended way, and
acknowledge that halo effects occur.
Source: Witoon Prinyawiwatkul, Ph.D., Robert Delaney, M. Michele Foley, Dustin Hilinski, and Howard R. Moskowitz, Ph.D.
c17.indd 359 1/14/2016 7:44:45 AM
360 • CULINOLOGY®
concern. The order of sample presentation should be bal-
anced and randomized. Balanced means that each sample
appears at a given serving position (first, second, third, and
so on) an equal number of times. Randomized indicates
that each order of presentation is randomly assigned to a
panelist. For example, three samples allow six possible
serving orders to achieve a balanced order of presentation
(ABC, ACB, BCA, BAC, CAB, CBA). If 60 panelists are in the
study, the six serving orders should each be randomly
assigned to ten of them.
3. Panel controls—Panelists should be screened for physical
conditions such as illness, food allergies, poor dental
hygiene, emotional distress, smoking, and drinking before
the test. To minimize confusion and errors, clear and con-
sistent instructions are required. The amount of training
needed for descriptive panelists varies depending on the
complexity of the products and the taste sensitivity required.
Applications of
Sensory Analysis
in Food Product
Development
The goal of the following case study was to identify new prod-
uct business opportunities for a pizzeria chain. The research
objectives were (1) to identify the types of sensory experience
pizza consumers wanted, and (2) to create better pizza prod-
ucts without expending an undue amount of money on cost
of goods.
Step 1: How Do People
Think? What Do People
Want in a Pizza?
What should a new pizza contain so the restaurant chain can
advertise it as “new” and “different,” yet maintain the essence
of the pizza chain? To identify what people want, short, easy‐
to‐understand concepts about pizza were developed. The
concepts comprised simple statements (elements) dealing
with the product, the eating situation, the emotions, and so
on. Some of the statements and their purchase interest scores
appear in Figure 17.6. To develop the language for the state-
ments, a focus group was used.
The actual study presented combinations of ideas to the
respondents, obtained ratings of the combinations (that
is, the test concepts), and then determined how much of
the response could be traced to each individual state-
ment. This method, called conjoint analysis, identifies the
contribution of the many factors that drive a product con-
cept.38 When looking at the impact of each statement, val-
ues above 5 are strong positives and values below 5 are
strong negatives.
Figure 17.6 summarizes data both from the total panel
and from two sub‐segments that were uncovered through a
FIGURE 17.6 Results from concept evaluations
showing what drives consumer interest and the
emergence of two consumer segments.
Segment 1
Stuff
Segment 2
Pure and
SimpleTotal
Base Size 119 69 50
Constant (overall
interest in “pizza”)
35 26 48
Soft and gooey slices
of pizza with cheese
15 25 2
Pizza with a filled or
twisted crust
10 17 0
With all the toppings
and accompaniments
you want
10 10 10
Pizza with a thick crust
and a rich topping
7 15 –4
Pizza with a crust that
doubles as breadstick
6 9 2
100% natural, fresh,
and carefully prepared
3 1 6
Pizza with a lot of
tomato sauce, ham,
and cheese
1 10 –12
You can just savor it,
when you think about it
1 1 1
Pizza is great for parties –1 –3 0
Thin crust pizza with a
layer of tomato sauce
and cheese
–2 7 –13
With a fresh mixed
salad
–2 2 –8
With a glass of
lemonade, beer, or wine
–3 –6 1
Pizza with a crust so
crispy you have to
be careful when you
eat it
–4 3 –13
Source: It! Ventures, LLC.
statistical segmentation of the total panel. Segmentation
groups people based on similar patterns of what they like.
The data suggested two distinct segments: One segment likes
pizza with “stuff,” and another segment likes pizza “pure and
simple.” Segment one is not interested in pizza at its very
basics (the baseline interest is low at 26). The second seg-
ment, a bit smaller, seems to prefer traditional pizza; it’s got
to have cheese, sauce with lots of richness and thickness, and
lots of crust.
c17.indd 360 1/14/2016 7:44:46 AM
CHAPTER 17 • Sensory Evaluation • 361
From this step, concepts can be written to further evalu-
ate the ideas, or prototypes can be created for consumer
evaluation. In this example, pizzas from competitors were
used to represent the ideas defined by the concept elements.
Step 2: What Works in Today’s
World of Pizza? Evaluating
Many Products to
Discover Patterns
Category appraisal studies evaluate a number of actual prod-
ucts to discover the characteristics most important to the
target consumer. For the pizza case, developers identified 10
types of pizza based on the conjoint analysis, some with dif-
ferent toppings but mainly concerned with different struc-
tures (thin versus thick crust, pizza with two layers, and so
on). The objective of testing different market products is sim-
ply to get an idea of what works in an assortment of actual
market products.
It is fairly straightforward to design and execute a category
appraisal, except for one thing: timing issues. With 10 pizzas
to test, all made to order for walk‐in customers at several piz-
zerias, it was necessary to create a run schedule so the test
panelists would always have pizza no more than 15 minutes
after purchase. The specific logistics are not relevant here,
other than to note that each of 100 pre‐recruited respondents
participated in a 2.5‐hour session in which each respondent
evaluated 5 of 10 freshly purchased pizzas. The pizzas repre-
sented different executions of cheese pizza from pizzerias in
a local area.
The most important outcomes are the patterns that exist
in the data, which can lead to insights and direction for devel-
opers. Figure 17.7 provides a snapshot of how 2 of these
10 pizzas performed. All the data are on a 0‐ to 100‐point
scale, making it easy to see the product on a scorecard.
The next analysis looks for patterns. For some sensory
attributes, as the intensity increases, liking increases, peaks,
and then goes down after the optimum sensory level is
passed. Saltiness and sweetness are examples of these types
of attributes; most people have levels where these attributes
are less liked because they are too low, well liked because they
are just right, and less liked because they are too high. The
goal is to determine what the curve looks like for each sen-
sory attribute of pizza, recognizing that many widely varying
attributes exist.
These types of analyses are important; they reveal the
dynamics of the product in a way that one might not intuit
and may not even be aware of without experience.39 Sensory‐
liking analyses work as long as the attribute is continuous (a
fundamental characteristic—pizza always has a crust) rather
than being on/off (for example, pepperoni present versus
absent).
Two examples of sensory‐liking curves are shown in
Figure 17.9. Each comes from the averaged ratings from the
participants who evaluated the 10 commercially prepared
pizzas. These curves were plotted to show ratings of breadiness
FIGURE 17.7 Representative data for two in‐
market pizzas on sensory and liking attributes.
Pizza sample number 101 102
General Liking
Overall liking 73 54
Appearance liking 77 56
Taste liking 73 56
Texture liking 70 53
Attribute Liking
Cheese 74 61
Crust 75 50
Edge 67 45
Middle 78 57
Sauce 56 56
Sauce color 59 56
Aroma 72 58
Cheese 73 53
Crust 73 56
Spiciness 62 52
(100-point scale where 0 = dislike and 100 = like)
Source: Witoon Prinyawiwatkul, Ph.D., Robert Delaney, M. Michele
Foley, Dustin Hilinski, and Howard R. Moskowitz, Ph.D.
of crust versus overall liking, and perceived amount of cheese
versus overall liking. Both indicate an optimum level in the
middle of the range tested.
Step 3: Nature Reveals Her
Secrets: Building and Using
the Product Model
Now the product developer positions all of the tested prod-
ucts on a map. The dimensions of the map are obtained from
the sensory attributes. Each of the 10 pizzas is located some-
where on this map. Products lying close together are similar
in their sensory properties.
The developer can now look at the map and identify the
gaps—namely, opportunities for new pizza products in areas
of the map that are not already occupied by other products
offered by competing pizza restaurants. In Figure 17.10, two
opportunity spaces are identified on the upper right side of
the map next to two pizza samples (G and E) that were well
liked (as indicated by the large circle).
The analysis then proceeds by creating product models,
which translate the opportunity spaces in the map into sen-
sory profiles. In practical application, each product model
defines the likely sensory profile of an optimum pizza that fits
each gap and looks for landmark products in the set of 10 piz-
zas that have the closest sensory levels. Different products
c17.indd 361 1/14/2016 7:44:46 AM
362 • CULINOLOGY®
Sensory Drivers of Liking
Shape Of Curve Interpretation
Upward Sloping As the amount of the sensory attribute increases, overall liking
increases.
Downward Sloping As the amount of the sensory attribute increases, overall liking
decreases.
Inverted U-Shape As the amount of the sensory attribute increases, overall liking
increases, then peaks, then decreases.
Flat Increasing amounts of the sensory attribute have no significant
effect on overall liking.
FIGURE 17.8 Typical sensory‐liking curves.
Source: Witoon Prinyawiwatkul, Ph.D., Robert Delaney, M. Michele Foley, Dustin Hilinski, and Howard R. Moskowitz, Ph.D.
How Sensory Attributes Drive Liking:
What Our Pizza Data Show
30
30 40 50 60
Crust - Breadiness
70 80
40
50
60
L
ik
in
g
70
80
30
30 40 50 60
Cheese - Amount
70 80
40
50
60
L
ik
in
g
70
80
FIGURE 17.9 Sensory‐liking curves for pizza, based on the category appraisal method.
Source: Witoon Prinyawiwatkul, Ph.D., Robert Delaney, M. Michele Foley, Dustin Hilinski, and Howard R. Moskowitz, Ph.D.
“Holes” In The Pizza Category
F
D G
E
A
B
C
I
H
Less
Overall
Liking More
Less
F
a
c
to
r
2
Factor 1
More
FIGURE 17.10 The sensory map. Each figure corresponds to one of the 10 pizzas, located in two‐
dimensional space defined by the sensory attributes.
Source: Witoon Prinyawiwatkul, Ph.D., Robert Delaney, M. Michele Foley, Dustin Hilinski, and Howard R. Moskowitz, Ph.D.
c17.indd 362 1/14/2016 7:44:47 AM
CHAPTER 17 • Sensory Evaluation • 363
may serve as landmarks because no one product may have all
the optimal sensory levels. For each sensory profile, compo-
nents of the landmark product (or combination, if there isn’t
a single best) become the developer’s or the chef ’s targets.
Compared to the best‐liked sample in this study, the opportu-
nity space is characterized by a pizza with a crisper crust, less
cheese, and more sauce. It has crust hardness similar to the
best‐liked sample.
Once the product model data is complete, the developer
or chef uses it to formulate prototypes characterized as hav-
ing the same structure and components. These prototypes
can be profiled by the sensory panel to compare their charac-
teristics to the opportunity profile and then tested with con-
sumers to determine their appeal. Of course, this is only one
step (albeit a critically important one) in the product devel-
opment process. See Chapters 1 and 19 for in‐depth discus-
sions about the business of new product development and
commercializing the gold standard.
Conclusion
As more and more food products flood the market, consum-
ers have ever more choices. The reach of store‐branded
private‐label offerings is always expanding. The outcome is
obvious; a competitive marketplace where brands fight for
limited space on the shelf. The bar of success for a new prod-
uct is constantly moving upward. Approximately 20,000 new
products were introduced each year for the last three years in
the United States. Of these new products, about 80 percent
were withdrawn within two years of launch. About 95 percent
of new product efforts fail to deliver expected business
objectives.
Manufacturers must incorporate that little added touch,
that bit of innovation, that extra dose of convenience, that
point of differentiation to ensure the success of their product.
The direction, insights, and inspiration for these extras
more often than not come from consumers. The more these
consumer touchpoints are incorporated into the product’s
research path, the better the chances for a successful prod-
uct. Sensory evaluation provides those touchpoints—from
the early observational and qualitative insights, to the trans-
lation of the chef ’s inspiration into a product developer’s pro-
totype, and finally to qualifying the commercialized formula
for mass production, product launch, and long‐term mainte-
nance in the marketplace. While nothing is guaranteed when
it comes to guessing the fickle taste preferences of the public,
a product development team can certainly stack the deck in
their product’s favor if they take the time to listen to and
learn from consumers.
1M. A. Amerine, R. M. Pangborn, and E. B. Roessler, Principles of Sensory
Evaluation of Food (New York: Academic Press, 1965), v.
2P. Dando, “Sensory Success: Quality, Consistency, and Sensory Programs,”
Stagnito’s New Products Magazine 8, no. 1 (2008): 68–70.
3K. S. Ferguson, “Foodservice New Products: ‘I Don’t Think We’re in
Commodities Anymore, Toto,’” in E. Graf and I. S. Saguy (eds.), Food Product
Development, From Concept to the Marketplace (New York: Chapman and
Hall, 1991), 291–308.
4D. Buck, “Methods to Understand Consumer Attitudes and Motivations in
Food Product Development,” in H. J. H. MacFie (ed.), Consumer‐Led Food
Product Development (Cambridge, UK: Woodhead, 2007), 141–157.
FIGURE 17.11 Specifications for development for the best pizza and for the new pizza that fits a hole
and therefore represents a new opportunity.
Pizza Attribute Scores
Best Liked
(Sample E)
Difference from Best Liked
for Opportunity
(Estimated from Model)
Direction for Product
Developer
Cheese: Amount 64 –16
Reduce cheeseCheese: Flavor 54 –11
Cheese: Stretchy 60 –15
Crust: Bready 51 +4
Similar crust breadiness
and hardness; more chewy
and crispy
Crust: Chewy 61 +9
Crust: Crispy 47 +13
Crust: Hardness 35 +0
Sauce: Amount 53 +13
More sauce; thicker sauce
Sauce: Italian Flavor 56 +17
Sauce: Spicy 52 +12
Sauce: Thickness 46 +10
Sauce: Flavor (Tomato) 52 +15
Source: Witoon Prinyawiwatkul, Ph.D., Robert Delaney, M. Michele Foley, Dustin Hilinski, and Howard R. Moskowitz, Ph.D.
c17.indd 363 1/14/2016 7:44:47 AM
364 • CULINOLOGY®
5J. Gordon, “Returning Insight to the Consumer,” Stagnito’s New Products
Magazine 6, no. 12 (2006): 40.
6M. Meilgaard, G. V. Civille, and B. T. Carr, Sensory Evaluation Techniques, 4th
ed. (Boca Raton, FL: CRC Press, 2007), 255–311.
7H. T. Lawless and H. Heyman, Sensory Evaluation of Food: Principles and
Practices (New York: Chapman and Hall, 1998), 519–547.
8A. Krystallis, “Using Means‐End Chains to Understand Consumers’
Knowledge Structures,” in H. J. H. MacFie (ed.), Consumer‐Led Food Product
Development (Cambridge, UK: Woodhead, 2007), 158–196.
9Meilgaard, Civille, and Carr, Sensory Evaluation Techniques.
10Buck, “Methods,” 141–157.
11Meilgaard, Civille, and Carr, Sensory Evaluation Techniques.
12W. Prinyawiwatkul and P. Chompreeda, “Applications of Discriminant
and Logistic Regression Analysis for Consumer Acceptance and Consumer‐
Oriented Product Optimization Study,” in J. H. Beckley et al. (eds.), Accelerating
New Food Product Design and Development (Ames, IA: Blackwell Professional,
2007), 271–295.
13Meilgaard, Civille, and Carr, Sensory Evaluation Techniques.
14Ibid.
15Amerine, Pangborn, and Roessler, Principles of Sensory Evaluation of Food,
540.
16R. Popper and J. J. Kroll, “Consumer Testing of Food Products Using
Children,” in H. J. H. MacFie (ed.), Consumer‐Led Food Product Development
(Cambridge, UK: Woodhead, 2007), 383–406; A. V. A. Resurreccion,
Consumer Sensory Testing for Product Development (Gaithersburg, MD:
Aspen, 1998), 165–176; H. Stone and J. L. Sidel, Sensory Evaluation Practices,
3rd ed. (San Diego, CA: Elsevier, 2004), 69–98.
17A. Chen, A. V. A. Resurreccion, and L. P. Paguio, “Age‐Appropriate Hedonic
Scales to Measure Food Preferences of Young Children,” Journal of Sensory
Studies 11 (1996): 141–163.
18Meilgaard, Civille, and Carr, Sensory Evaluation Techniques.
19S. C. King, H. L. Meiselman, and B. T. Carr, “Measuring Emotions Associated
with Foods: Important Elements of Questionnaire and Test Design,” Food
Quality and Preference 28 (2013): 8–16.
20Silvia C. King, Herbert L. Meiselman, and B. Thomas Carr, “Measuring
Emotions Associated with Foods in Consumer Testing,” Food Quality and
Preference 21 (2010): 1114–1116; King, Meiselman, and Carr, “Measuring
Emotions Associated with Foods: Important Elements of Questionnaire
and Test Design.”
21Y. Jiang, J. M. King, and W. Prinyawiwatkul, “A Review of Measurement and
Relationships Between Food, Eating Behavior, and Emotion,” Trends in
Food Science and Technology 36 (2014): 15–28.
22A. V. Cardello et al., “Measuring Emotional Responses to Foods and Food
Names Using Questionnaires,” Food Quality and Preference 24 (2012): 243–250.
23See Meilgaard, Civille, and Carr, Sensory Evaluation Techniques, 63–128, for
in‐depth descriptions of sensory discrimination tests, along with exam-
ples describing scope and application, test subjects, test procedures, data
analysis, and interpretation of the results.
24J. Bi, Sensory Discrimination Tests and Measurements: Statistical Principles,
Procedures, and Tables (Ames, IA: Blackwell, 2006), ix.
25G. V. Civille and H. T. Lawless, “The Importance of Language in Describing
Perceptions,” Journal of Sensory Studies 1 (1986): 217–236.
26Amerine, Pangborn, and Roessler, Principles of Sensory Evaluation of
Food, 349–397; E. Chamber and B. M. Wolf, Sensory Testing Methods, 2nd ed.
(West Conshohocken, PA: ASTM, 1996), 58–72; R. C. Hootman, Manual on
Descriptive Analysis Testing for Sensory Evaluation (West Conshohocken,
PA: ASTM, 1992); Meilgaard, Civille, and Carr, Sensory Evaluation
Techniques, 173–253; Stone and Sidel, Sensory Evaluation Practices,
201–245.
27M. C. Gacula Jr., Descriptive Sensory Analysis in Practice (Trumbull, CT: Food
and Nutrition Press, 1997).
28G. V. Civille and B. G. Lyon, Aroma and Flavor Lexicon for Sensory Evaluation:
Terms, Definitions, References and Examples (West Conshohocken, PA:
ASTM, 1996).
29S. Clark, M. Costello, M. Drake, and F. Bodyfelt, The Sensory Evaluation of
Daily Products (New York: Springer Science & Business Media, 2009).
30Meilgaard, Civille, and Carr, Sensory Evaluation Techniques Food, 7–24.
31A. V. Cardello, “Consumer Expectations and Their Role in Food Acceptance,”
in H. J. H. MacFie and D. M. H. Thomson (eds.), Measurement of
Food Preferences (London: Blackie Academic, 1994), 253–297; D. B.
MacDougall, “Instrumental Assessment of the Appearance of Foods,”
in A. A. William and R. K. Atkin (eds.), Sensory Quality in Foods and
Beverages: Definition, Measurement, and Control (Deerfield Beach, FL:
Verlag Chemie International, 1983), 121–139.
32J. B. Hatchings, “The Importance of Visual Appearance of Foods to the Food
Processor and Consumer,” in G. G. Birch, J. G. Bremer, and K. J. Parker (eds.),
Sensory Properties of Foods (Barking, London: Applied Science, 1977),
45–56; Meilgaard, Civille, and Carr, Sensory Evaluation Techniques, 7–24.
33W. Prinyawiwatkul, L. R. Beuchat, and A. V. A. Resurreccion, “Optimization
of Sensory Qualities of an Extruded Snack Based on Cornstarch and
Peanut Flour,” Lebensmittel-Wissenschaft & Technologie 26 (1993): 393–399.
34Meilgaard, Civille, and Carr, Sensory Evaluation Techniques, 7–24.
35Lawless and Heyman, Sensory Evaluation of Food, 28–81.
36E. Larmond, “Is Sensory Evaluation a Science?” Cereal Foods World 39, no.
11 (1994): 804–808.
37Meilgaard, Civille, and Carr, Sensory Evaluation Techniques, 25–38.
38H. R. Moskowitz et al., “Rapid, Inexpensive, Actionable Concept Generation
and Optimization: The Use and Promise of Self‐Authoring Conjoint
Analysis for the Foodservice Industry,” Food Service Technology 1 (2001):
149–168.
39H. R. Moskowitz, “Improving the ‘Actionability’ of Product Tests: Under-
standing and Using Relations Among Liking, Sensory, and Directional
Attributes,” Canadian Journal of Marketing Research 18 (1999): 31–45;
H. R. Moskowitz, “Relative Importance of Perceptual Factors to Consumer
Acceptance: Linear Versus Quadratic Analysis,” Journal of Food Science 46
(1981): 244–248.
c17.indd 364 1/14/2016 7:44:47 AM
CHAPTER 18 • Culinology® Applications in Food Processing • 365
18
Culinology®
Applications in Food
Processing—From
the Chef ’ s and
Food Scientist ’ s
Perspective
Introduction: Today ’ s
Case for Processed
Foods—Scientific
and Culinary
Perspectives
Foods have been prepared and processed since the begin-
ning of recorded history. Preserving food enables a consist-
ent supply during periods of decreased availability (seasonal
variability, drought, natural disaster, and famine). Further,
the centralized processing of food had a profound impact on
civilization in that it provided a steadier, more consistent
supply of cheaper foods. People were thus able to spend less
time on the daily procurement and preparation of food.
Centralized processing also supported the move from agri-
cultural to industrial economies, as foods could be made
more easily available to city‐dwelling factory workers and
their families.
The current dialogue among journalists, social commen-
tators, and consumers suggests a profound dislike and dis-
trust of processed foods. These range from well‐informed
presentations to more emotional diatribes. The popular press
is replete with examples of direct confrontations with and
denigration of processed foods.
By changing from consuming chemically ridden mass‐
produced food to eating locally grown organic food, we can
give ourselves a longer and healthier life.
Yoko Ono, musician and cofounder (with John Lennon in 1969)
of Plastic Ono Band (Anon., 2009)
Although increasing food choices for the consumer is
desirable, such a call for massive restructuring of the food
industry would have a profound negative impact on overall
food availability, safety, and economics.
The Culinology® professional (and the consumer) benefits
directly from access to diverse commercially prepared and
processed food ingredients to provide sophisticated meal
offerings in numerous formats. Many of these ingredients
play such a ubiquitous part in our diet that they are no longer
perceived as processed foods but rather are considered ordi-
nary ingredients found in everyone ’ s home pantry. Even the
most ardent critics of processed foods are unlikely to reach
for a live steer when seeking a steak dinner, or to grow and
mill their own whole grains to make flour. Corn oil doesn ’ t
normally drip from ears of corn! Commercial processing of
foods provides stable, convenient, safe, and nutritious
options. Knowledge of food technology increasingly serves
the needs of culinary specialists who require a solid under-
standing of the principles and procedures used for food proc-
essing and preservation.
Lead Author: Mark A. Uebersax, Ph.D. , Professor Emeritus, Department of Food Science
and Human Nutrition , Michigan State University
Muhammad Siddiq, Ph.D. , Associate Professor, Department of Food Science and Human
Nutrition , Michigan State University
Carl P. Borchgrevink, Ph.D. , Associate Professor, The School of Hospitality Business, The Eli
Broad Graduate School of Management , Michigan State University
c18.indd 365 1/22/2016 1:20:40 PM
366 CULINOLOGY®
FIGURE 18.1 Ubiquitous processed foods; “neo‐scratch” foods.
Food in Unprocessed Form1 Minimally or Fully Processed Further Processed
Tomatoes Blanched and peeled Chopped and diced
Chopped, diced, and
seasoned
Paste, purée, sauce
Mustard Ground mustard Prepared mustard
Grains Meals and flours Enriched flours
Dough
Proofed dough
Par‐baked dough products
RTE dough products
Puff pastry/phyllo dough
Stocks and broths Food bases Sauce mixes
Soup bases Soup mixes
Heat‐and‐serve soup
Raw milks Pasteurized milks Butter
Homogenized milks Yogurt, kefir
Cheese
Spices and herbs Dried and irradiated whole spices and herbs Ground, chopped, minced spices
and herbs
Vanilla bean Fermented and dried vanilla bean Ground vanilla beans
Vanilla seeds
Vanilla extract
Vanilla sugars
Vegetables Ready to use IQF
Block frozen
Canned
Dried
Dried and reconstituted
Coffee beans Roasted coffee beans Ground coffee beans
Coffee extracts
RTD coffees
Cattle, hogs, sheep, goats Carcass Primal cuts
Partial carcass Fabricated cuts
Pre‐marked fabricated cuts
Pre‐cooked fabricated cuts
(continued)
c18.indd 366 1/14/2016 9:35:53 PM
CHAPTER 18 Culinology® Applications in Food Processing 367
Food in Unprocessed Form1 Minimally or Fully Processed Further Processed
Ground products
Pre‐formed patties
Pre‐cooked patties
Sausages
Pre‐cooked sausages
Whole fish Gutted Fish cakes
Dressed‐eviscerated Fish fingers
Head‐on or headless Dehydrated fish powder
Fins‐on or finless
Scaled
Skinned
Filleted
Steaks
1We assume that the food in question is free of dirt and debris; such cleaning could be considered an initial level of processing.
Source: Mark A. Uebersax, Ph.D., Muhammad Siddiq, Ph.D., Carl P. Borchgrevink, Ph.D.
FIGURE 18.1 (continued)
The relationship between food, diet, exercise, and health
has been clearly established with sound scientific consen-
sus. Food scientists, Culinology® professionals, nutrition-
ists, and public health and policy professionals all have an
obvious mandate to exploit these complex interactions for
the benefit of consumers. This task is seemingly monumen-
tal and must be approached as a multifaceted endeavor.
The entire food system, from farm to fork, must be taken
into account to achieve a safe and wholesome food supply.
The food processing and preservation segment of this
system is a vital link that the Culinology® professional
must recognize and appreciate. World food security and
availability can be realized only through strategies for
minimizing postharvest losses, expanding preservation
and packaging technologies, and collaboration among food
professionals.
Effects of Processing
on Nutrient Retention
Retention of nutrients during processing is important and
has been studied extensively. Nutrient losses occur whether
food is processed commercially or prepared fresh at home or
at foodservice operations. Though behavior varies among
specific foods, the losses due to commercial processing of
foods are not dramatically different from fresh cooked foods.
In fact, when processed using the latest high‐efficiency tech-
nologies, and with the potential for food fortification or
enrichment, such foods can be much more nutritious than
home‐processed foods.
A common misconception among journalists as well
as consumers is that commercially processed foods are
inferior to fresh products or to foods processed in the
home. The following summarizes studies done in this
area.1
Decreases in nutrient levels of foods can occur for a vari-
ety of reasons at all stages of the food distribution chain,
from harvest to the point of consumption. Less well under-
stood is the positive impact processing frequently has on
improved levels of nutrient extraction (for example, more
lycopene in tomato paste than in fresh tomatoes), enhanced
nutrient bioavailability (for example, protein digestibility),
and inactivation of anti‐nutritional factors (for example,
trypsin inhibitors in raw eggs and toxic lectins in legumes).
Nutrient losses associated with processed food, which are
often similar to those of kitchen‐prepared foods, may be clas-
sified as follows:
Intentional losses—Losses that result from the processing or
conversion of raw materials into an edible portion are consid-
ered intentional losses. Included in this group are peeling,
trimming, and coring losses.
Leaching of water‐soluble nutrients—Leaching of soluble vita-
mins and minerals causes most of the nutrient losses.
Washing and blanching are operations in which most leach-
ing occurs. Cutting causes cellular disruption and increases
c18.indd 367 1/14/2016 9:35:53 PM
368 CULINOLOGY®
degradation. Oxidative losses are readily controlled and mini-
mized through optimization of processing procedures—for
example, processing under a vacuum allows for lower tem-
peratures, which are less destructive to sensitive vitamins;
flushing with inert gas (nitrogen) eliminates oxygen. Thermal
processing also inactivates many oxidative enzymes—for
example, ascorbic acid oxidase and lipoxygenases.
Thermal degradation—Heat‐sensitive vitamins and bioac-
tive compounds (antioxidants) are reduced as a direct result
of heating. This loss occurs, of course, during any heating or
cooking of foods. However, thermal treatments also improve
product stability, market availability, and safety by inactivat-
ing deleterious enzymes and microorganisms. Further, using
such high‐tech thermal treatments as HTST (high tem-
perature, short time) and UHT (ultra‐high temperature)
processing can minimize thermal degradation to a great
degree by reducing exposure time. In addition, nutrient for-
tification can be used to add back nutrients lost during
processing.
Market Fresh vs. Quick Frozen
Typically, fresh fruits and vegetables are considered to
possess an optimum level of nutrients and high‐quality
attributes. This may be the case immediately following har-
vest; however, “harvest fresh” may be dramatically different
from “market fresh,” which is frequently two to three weeks
Fresh = 100
Blanching
75
75
71
63 70
Thawing Heating Cooking
Cooking
Canning Freeze drying
44 39 36 35
Cooking Freezing
FIGURE 18.3 Percentage of vitamin C retained in
peas by current processing and cooking methods.
FIGURE 18.2 General effects of commercial
processing on various nutrients.
Nutrient Processing Effects
Lipids Oxidation accelerated by light
Protein Denatured by heat (improves
digestion)
Amino acids Some are sensitive to light. Lysine
bio‐availability reduced by
non‐enzymatic browning
Vitamin C
(Ascorbic acid)
Decreases during storage, drying,
heating, oxidation, cell damage
(e.g., chopping or slicing)
Stable to heat under acidic
conditions
Vitamin B
1
(Thiamine)
Destroyed by high temperatures,
neutral and alkaline (e.g., baking
soda, baking powder) conditions
Lost in cooking water
Vitamin B
2
(Riboflavin)
Sensitive to light at neutral and
alkaline conditions
Moderately heat stable under neu-
tral conditions
Sensitive to heat under alkaline
conditions
Vitamin B
3
(Niacin,
Nicotinamide)
The most stable vitamin
Stable to heat and light
Leaches into cooking water
Folate Decreases with storage or pro-
longed heating
Lost in cooking water
Destroyed by use of copper utensils
Vitamin B
6
(Pyridoxine,
Pyridoxal)
Heat stable in alkaline and acidic
conditions
Pyridoxal is heat labile
Vitamin B
12
Destroyed by light and high pH
Carotenes Easily destroyed by heat
Oxidize and isomerize when ex-
posed to heat and light
Vitamin A Very heat labile; easily destroyed
by heat
Easily oxidized
Vitamin D Oxidizes when exposed to heat
and light
Vitamin E Oxidizes readily
Source: Morris et al. (2004).
the product’s surface area exposed to water, thus further con-
tributing to the loss of nutrients.
Oxidative and enzymatic losses—Labile vitamins, such as
ascorbic acid, are often lost via oxidation or enzymatic
Source: Christian and Greger (1988).
c18.indd 368 1/14/2016 9:35:54 PM
CHAPTER 18 Culinology® Applications in Food Processing 369
post‐harvest. Commercially quick‐frozen products, which
are generally processed within 24 hours of harvest, may actu-
ally have higher net nutrient content and offer added benefits
of availability, shelf stability, and convenience.
Traditional Food
Processing Technologies
Preparation and Size Reduction
Commercial food processing requires large quantities of
commodities from various agricultural sectors: fruits and
vegetables, cereal grains and legumes, meat and poultry, eggs
and dairy products, fats and oils, and seafood. These must be
handled rapidly and in a manner that assures food safety and
quality.
Generally, food commodities that require washing are
washed with chlorinated water or with other added sanitizers
(for example, ozone, peroxyacetic acid, or hydrogen peroxide).
Improved washing systems use high‐pressure mechanical
brushes and countercurrent flow principles (cleanest water in
contact with cleanest product). The objective is to remove
FIGURE 18.4 Key preparatory operations
for various categories of foods.
Food Category Key Preparatory Operations
Fruit and vegetables
(including herbs and
spices)
Manual and mechanical harvest
Cooling (removal of field heat)
Clean and wash
Peel, sort, trim
Slice/dice
Cereal grains and
legumes
Harvest
Cleaning (air aspiration and sieving)
Drying to stable moisture content
Meat and poultry Slaughter and evisceration
Washing
Chilling
Carcass hanging/aging
Primal and retail cuts
Dairy products Milking
Chilling
Bulk receipt and delivery
Shell eggs Collection
Washing
Inspecting
Grading
Seafood Catching
Washing
Chilling/icing
Cutting/filleting
Source: Mark A. Uebersax, Ph.D., Muhammad Siddiq, Ph.D.,
Carl P. Borchgrevink, Ph.D.
FIGURE 18.5 French knife cut terminology.
French
Culinary Term Parameters
Émincé Thinly sliced
Chiffonade Rolled leaves cut ultra thin
Rondelle Disk‐shaped slice, a.k.a. coin
Gaufrette Rondelle or bias cut with a ridged blade
Brunoise 1⁄8th‐inch cube
Macédoine ¼‐inch cube
Parmentier ½‐inch cube
Carré ¾‐inch cube
Paysanne Rough “farmers” cut; often considered
½ by ½ by 1⁄8th–1⁄4 inch
Julienne 1⁄8th by 1⁄8th by 2–2.5 inches
Allumette 1⁄8th by 1⁄8th by 3⁄4 inch or 1⁄16th by 1⁄8th by
1.5 inches
Batonnet ¼ by ¼ by 2–2.5 inches
Frite ½ by ½ by 3 inches
Pont Neuf ¾ by ¾ by 3 inches
Tourné (m) or
Tournée (f)
8–16 sided blunt-end football shape,
a.k.a. turned
Château Turned to the size of a large olive
à l’Anglaise Turned to the size of a pigeon’s egg or a
large clove of garlic
Fondante Turned to the size of a regular (large) egg
Parisienne 1¾‐inch diameter ball
Noisette Ball the size of a hazelnut
Source: Escoffier (1934, 1969); Ranhofer (1894).
natural and incidental contaminants and debris (for example,
soil, manure, stones, organic matter, insects, and rubbish).
After washing and sorting, raw materials undergo peeling,
trimming, and size‐reduction operations. These frequently
require sophisticated machinery that can handle high capac-
ity and is flexible for use with various types of food. This
equipment is most often fabricated in stainless steel and
according to principles of sanitary design.
From the perspective of the chef, size reduction, as in minc-
ing, dicing, and chopping, is essential to the preparation and
delivery of high‐quality foods. Uniformity of size and shape
lends itself to even cooking and desirable visual appeal. In the
culinary world, many approaches to size reduction use descrip-
tive French terms that have a rather precise definition.
Commercial processing equipment can be used to repro-
duce many of these precise cuts; however, the typical acceler-
ated throughput of commercial operations can detract from
precision in size and shape consistency. There is always an
c18.indd 369 1/14/2016 9:35:54 PM
370 CULINOLOGY®
element of compromise in high‐volume production com-
pared to what is possible in a restaurant setting. That said,
the same commercial processing equipment typically found
in a high‐volume commercial kitchen—slicers, buffalo chop-
pers, food processors, blenders, and meat grinders—can be
used successfully in the product development lab or test
kitchen as well.
Minimally Processed
Fresh foods
Minimally processed or fresh‐cut produce includes any fresh
fruit, vegetable, or combination thereof that is physically
altered from its original form but remains in a fresh state.
Processing in this category has two main objectives:
1. Keeping the produce fresh by extending its shelf life with-
out losing its nutritional and sensory quality or compro-
mising product safety.
2. Ensuring a product shelf life sufficient to make its distribu-
tion feasible within the region of its consumption.2
Fresh‐cut produce has been one of the fastest‐growing
segments of the U.S. food industry in the last few years.3
Quality of fresh‐cut products depends on visual appear-
ance (freshness, color, defects, and decay), texture (crispness,
turgidity, firmness, and tissue integrity), flavor (taste and
smell), nutritive value (vitamins, minerals, and dietary fiber),
and safety (absence of chemical residues and microbial con-
tamination).4 From the consumers’ perspective, the color and
appearance of fresh‐cut produce has the greatest impact on
purchase intent. If the color of a fresh‐cut product is not
attractive or of acceptable perceived quality, the consumer is
less likely to purchase it regardless of its excellent texture, fla-
vor, taste, or other quality attributes.5
The degradation of color in fresh‐cut produce is primarily
the result of two enzymes: polyphenol oxidase and peroxi-
dase. These enzymes also impair aroma and flavor and,
hence, the marketability of the product. In addition, they
can reduce nutritive value via oxidation of ascorbic acid
(vitamin C).6 Traditionally, sulfites were used extensively in
the food industry to control color degradation or browning.
However, since the late 1980s scientists have been seeking
replacements for sulfites due to adverse respiratory reac-
tions in individuals with asthma.7 When safety issues were
raised in 1984, the U.S. Food and Drug Administration
(FDA) banned sulfite use in fresh‐cut fruits and vegetables
in salad bars. A number of alternatives, such as ascorbic
acid, citric acid, 4‐hexylresorcinol, erythorbic acid and
sodium erythorbate (stereoisomers of ascorbates), benzoic
acid, and natural fruit juices (for example, lemon juice) are
commercially available and yield varying degrees of success,
either alone or in combinations. The addition of organic
acids (for example, citric acid, ascorbic acid) lowers pH and
thus limits enzymatic action, as enzymes rapidly lose their
catalytic effectiveness in an acidic environment.
In addition to chemical anti‐browning treatments, other
technologies, such as modified atmosphere packaging (MAP),
are widely used. In this approach, the normal ratio of gases in
air (21 percent oxygen and 0.038 percent carbon dioxide) is
adjusted to reduce the oxygen level to 5 to 10 percent and
elevate the carbon dioxide to 5 to 20 percent inside the pack-
age.8 Limiting oxygen and increasing carbon dioxide dramati-
cally reduces the rate of many biological reactions, including
oxidation and rancidity, enzymatic browning, cellular respi-
ration, and growth of microorganisms, the result of which
provides extended shelf life to cut produce (and other foods
as well).
Thermal Processing
The objective of thermal processing (blanching, pasteuriza-
tion, canning, aseptic processing and packaging) is to help
stabilize a food product, ensure it is wholesome and safe for
consumption, and yield sufficient shelf life to satisfy the
needs of the business and the consumer.
Microorganisms are inherent to all raw food commodities
and are categorized as either spoilage or pathogenic (disease‐
causing) organisms. Enzymes are proteins that catalyze
metabolic reactions in all living plants and animals. They are
present in all raw foods. If they are not inactivated, they cause
continued breakdown and deterioration of food materials
until the food is no longer desirable for consumption. In
addition to controlling microorganisms, thermal processes
are also used to denature enzymes in foods to prevent
deterioration.
Thermal processes can be generally divided into two
types: those that provide short‐term inhibition of microbial
growth and enzymatic activity (blanching and pasteuriza-
tion) but require additional inhibitory methods (dehydration,
refrigeration, freezing, chemical preservatives) to provide
longer shelf life; and those entirely designed to provide long‐
term shelf stability at room temperature (canning and asep-
tic processing).
Blanching
Blanching is preliminary to many basic methods of food
preservation—canning, freezing, dehydration, and so on. The
terminology is derived from the French word blanche,
meaning “white.” Among other effects, blanching inactivates
oxidative enzymes and thus stabilizes the natural appearance
of many fruits and vegetables. This control of browning was
perceived as having a whitening effect on the product, hence
the term.
Blanching is a relatively mild heat treatment given to raw
products before they are packed into containers for canning
or freezing. It is a critical step in stabilizing many quality
attributes. It may be accomplished by steam or hot water and
serves as a final wash to reduce microbial load, inactivate
enzymes, remove certain raw or off flavors, improve color
and flavor retention (especially in frozen foods), and increase
the pliability of the product. Blanching also facilitates filling
by expelling dissolved or occluded gases from the plant
tissues.
c18.indd 370 1/14/2016 9:35:54 PM
CHAPTER 18 Culinology® Applications in Food Processing 371
In hot‐water systems, the product must be totally sub-
merged in water at or above 190°F (88°C) for a prescribed
time (dependent on the type and size of the food). These
methods may use static water baths or continuous systems
where screws or reels move the product through the hot
water at a controlled rate. Recently, tubular pipe blanchers,
which require pumping the product in water through a pipe
in which steam is injected, have been used to improve blanch-
ing capacity and efficiency. While water systems provide
desirable quality control and rapid uniform heat transfer, this
type of blanching generally results in relatively high leaching
losses of soluble nutrients (vitamins, minerals) and flavor
components. In addition, water‐blanching operations pro-
duce large volumes of waste water. By contrast, steam blanch-
ing reduces both nutrient losses and water requirements and
has thus found wide use in the industry.
Pasteurization
Pasteurization is a thermal treatment that provides increased
refrigerated shelf stability and reduces public health hazards
(pathogens). Pasteurization is named for Louis Pasteur, the
French scientist who first identified microorganisms associ-
ated with fermentation and spoilage. The heat treatment
may be applied directly to the product prior to packaging
(milk or juice) or after packaging (high‐acid [below pH 4.6]
foods such as pickles). Pasteurization after packaging elimi-
nates the possibility of recontamination of pasteurized prod-
uct before it reaches the protection of the package.
This reduced heat treatment does not result in commercial
sterility but rather is designed to destroy only pathogenic
microorganisms and inactivate deteriorative enzymes. Unlike
Pasteurization Processes
for Selected Foods9
Milk
USDA pasteurization parameters (first three require refriger-
ation after processing):
143°F (62°C)/30 minutes—vat pasteurization (earliest
method)
161°F (72°C)/17 seconds—HTST (high temperature, short
time); most common method used in the United States
280°F (138°C)/2 seconds or longer—ultrapasteurization
Ultrapasteurization combined with aseptic processing and
packaging (also known as UHT [ultra‐high‐temperature
processing]). Unlike the former methods, this is a form of
sterilization; these products do not require refrigeration
until they are opened.
Milk pasteurization is based on achieving a 12‐D reduction of
Coxiella burnetti, the most heat‐resistant pathogen found in
milk. The D‐value (decimal reduction time) is a measure of the
heat resistance of a microorganism. It is the time in minutes at
a given temperature required to destroy 1 log cycle (90 percent)
of the target microorganism. Thus, after a bacterial population
is reduced by 1‐D, only 10 percent of the original organisms
remain. 12‐D equals sufficient heat to reduce organism popu-
lations by 12 log cycles.
Alkaline phosphatase, a naturally occurring enzyme in milk,
possesses a similar D value to heat‐resistant pathogens and
is generally used as a simple colorimetric confirmation test
for effective pasteurization. If the enzyme is no longer
active, pasteurization is complete and the milk is safe for
consumption.
Eggs
USDA guidelines for fluid egg products:
Salmonellae—Objective is to achieve a 9‐D reduction in
S. seftenberg; 140°F (60°C)/3½ to 4 minutes.
Pasteurization temperature is below albumen’s (egg white)
denaturation temperature range in order to keep the eggs
from curdling; similar to the alkaline phosphatase test used
to measure pasteurization success in milk, α‐amylase activity
is used as a convenient colorimetric test for measuring the
effectiveness of egg pasteurization.
Apple Juice and Cider
170 to 200°F (77 to 93°C)/1 to 3 minutes
Bottled Beer
Most beer in cans and bottles is pasteurized; the pasteuri-
zation process occurs after the beer is placed in the can or
bottle and the container is sealed. The process involves
running the containers through a hot‐water spray (approxi-
mately 140°F (60°C) for 2 to 3 minutes. American keg beer
(draft beer) is not pasteurized (although imported keg beer
is) and must be stored refrigerated at 38°F (3°C).
Fermented or Acidified Products (Pickles,
Sauerkraut, Fruits)
These are products whose pH level is below 4.6, due to either
fermentation or the direct addition of acids (acidification).
Due to their acidity, it is rare to find pathogens; the usual
culprits here are spoilage organisms. Typical thermal proc-
essing for these products is known as hot‐fill‐and‐hold,
whereby the product is heated to between 180 and 212°F
(82 and 100°C) before filling, then pumped hot into sani-
tized containers, sealed, and inverted for 3 minutes to steri-
lize the lid and develop a vacuum before cooling. Alternatively,
the hot‐filled and capped jars can be processed after filling
in a pasteurization tunnel that will expose the jars to hot
water of at least 190°F for sufficient time (which will differ
depending on the size of the jars and the type of product) to
assure commercial sterility. This is analogous to the hot‐
water‐bath method used by home canners for decades.
Fruit Juice Products
It is fairly easy to hot‐fill juices by rapidly heating the juice in a
heat exchanger and filling containers with the hot juice (around
203°F [95°C]), followed by sealing and inverting, thus pasteur-
izing the container. Reasonably rapid cooling is accomplished
by rotary or spin action. This is known as flash pasteurization
and can be achieved almost instantaneously.
c18.indd 371 1/14/2016 9:35:54 PM
372 CULINOLOGY®
retorted products, which are stable at room temperature,
pasteurized products must be refrigerated post‐treatment to
inhibit the outgrowth of spoilage organisms that survive the
process. Pasteurization processes are regulated by federal
authorities—U.S. Public Health Service (USPHS), FDA, U.S.
Department of Agriculture (USDA)—and are typically achieved
with heat sufficient for a specified reduction in the population
of a specific test organism within a specific food.
Conventional Canning (Retorting)
Microorganisms consist primarily of vegetative (living) cells
that are relatively heat‐labile. However, many micro organisms
(for example, Clostridium botulinum) can form dormant, heat‐
resistant spores when their environment changes to the point
where vegetative cells can’t survive. The spores can remain
dormant for as long as it takes for the surroundings to revert
to the appropriate conditions for germination, and then the
vegetative population is resurrected. For extended room‐
temperature storage of foods, thermal processing must pro-
vide sufficient heating temperatures and processing time to
inhibit or inactivate the bacterial spores as well as vegetative
cells. The thermal resistance of spores varies with each type of
organism, the spore concentration, and the chemical nature
(salt, sugar, protein, acidity, and pH) of the food. Determining
the requirements sufficient to establish commercial sterility
requires knowledge of the heat resistance of spores and of the
manner in which heat penetrates the food.
Commercial sterility is a compromise based on the practi-
cal impossibility of achieving complete sterility in the con-
tents of a hermetically sealed (airtight) container during
commercial thermal processing. While it is recognized that
the possibility exists for a bacterium to survive the process,
the chances of even a single organism surviving are extremely
low if the thermal process has been designed correctly.
Achieving commercial sterility in hermetically sealed con-
tainers of low-acid foods (pH > 4.6) processed for ambient
(room temperature) storage is critically important, as the
pathogen most likely to grow in these types of foods is
Clostridium botulinum, the spores of which release a potent
neurotoxin if allowed to germinate.
The common terminology used for thermal processing of
foods in hermetically sealed containers is retorting (also
called canning). This technology is relatively new (early 1800s)
compared with more primitive methods such as smoking, salt-
ing, pickling, and dehydration. Large‐scale canning of food
was first successfully performed by Nicholas Appert, a French
confectioner who responded to Napoleon’s call for an improved
method of preserving food for his army. Appert’s success was
achieved after numerous trial‐and‐error attempts at filling
bottles with food, sealing them with corks, and heating them
in boiling water for various periods.10
Canning is one of the most widely used methods for long‐
term preservation of a wide range of foods, including meat,
fish, poultry, milk products, fruits, vegetables, and complex
formulated foods (soups, stews, pasta, sauces, and pastes).
Canning is a high‐volume, efficient, and safe means of
stabilizing food in a convenient, fully cooked, and cost‐effec-
tive manner. Modern canneries can process 1000 cans per
minute (16 to 17 cans per second). In addition to actual cans,
canning is also used to successfully process foods in glass
jars, flexible pouches, trays, and other packages that are
capable of withstanding the high temperature (250°F [121°C])
and pressure (15 psi) required to achieve it.
In the modern process, following pre‐processing prepara-
tion procedures (including washing, sizing, mixing, seasoning,
and blanching or cooking), products are immediately put into
containers and sealed. Systems range from simple, manual fill-
ing to highly engineered automated machinery. Control of net
weight must be maintained for regulatory compliance (weights
and measures) and to assure consistent heating in every con-
tainer. Foods to be packed in brines or syrups are put into con-
tainers first and then covered with the liquid portion. Filled
containers are then exhausted by being passed through a hot‐
water bath or steam chamber to expel any trapped gases and
promote expansion of the filled product before the can is
sealed; this is necessary to ensure a full vacuum on cooling.
The exhausted can is then hermetically sealed by double‐
seaming a lid to it. This operation is a critical control point
(CCP) in the process. Detailed attention to the dimensions and
security of the seam is required to assure food safety.
Each step in the processing of fresh crops into stable
canned products is characterized as a unit operation.
Although specific requirements may vary for different prod-
ucts, overall these are fairly typical operations.
The processor attains commercial sterility by eliminating
all pathogens and microorganisms that cause spoilage in
cans (or similar containers) that will be handled under ambi-
ent temperatures (for example, distributed, marketed, and
stored at room temperature).11 Survivor curves for microor-
ganisms are constructed to determine the time and tempera-
ture required to give a desired reduction in the microorganisms’
population. The D‐value (decimal reduction time) is used to
specify the time in minutes at a specific temperature required
for a 1 log reduction (a tenfold reduction) in the population of
a given microorganism.
Note: There are five layers of steel folded and compressed with a sealing
compound to form the hermetic double seam.
FIGURE 18.6 Cross‐section of a can double seam.
Source: USDA‐FSIS, Center for Learning (2005).
c18.indd 372 1/14/2016 9:35:54 PM
CHAPTER 18 Culinology® Applications in Food Processing 373
All factors affecting heat penetration must be known in
order to calculate the time and temperature requirements for
achieving commercial sterility. Heating profiles of canned
foods must be measured at the slowest heating point (cold
spot) of the food within the container. Heat transfer is deter-
mined by placing a thermocouple (sensor used to measure
temperature) at the cold point of the container (typically the
geometric center) and monitoring the temperature change
during heating. External heat may penetrate to the cold spot
by conduction or convection (or both). Typically, solid‐
packed foods are heated primarily by conduction, whereas
liquids are heated by convection, simply because of differ-
ences in the heat transfer dynamics between solids and liq-
uids. Heating characteristics are also dramatically influenced
by mechanical agitation of the containers during retort oper-
ation, which increases the heat transfer efficiency of the
system.
During canning, heating rate is influenced by several
factors:
The material of the container
The size and shape of the container
The initial temperature of the product prior to heating
The processing temperature
The physical consistency or size configuration of discrete
food particles.12
Acid food products (pH < 4.6) in hermetically sealed con-
tainers may simply be heated in boiling‐water baths for rela-
tively brief times to achieve commercial sterility, as their high
acidity provides enough additional inhibitory protection to
assure safety. Atmospheric water processing systems for acid
foods may be of relatively simple design; however, they must
provide uniform temperature control. Numerous continu-
ous‐processing systems are commercially available for the
required controlled heating of acid foods.
On the other hand, low‐acid foods (pH > 4.6) require proc-
essing temperatures in excess of 212°F (100°C) to destroy
Clostridium botulinum spores. These temperatures are attaina-
ble only in pressurized steam retorts or pressure cookers. Several
types of thermal processing systems are commercially available
and have a high degree of safety compliance. These include:
Discontinuous retorts—These are batch types, classified as
either still or agitating. Still retorts are static pressure cookers
normally operated at 240 to 250°F (115 to 121°C) (10 to
15 psi steam pressure) and provide no agitation of the can
during processing. The sealed cans are placed inside the
retort, air is vented from the retort with steam, and pressure
is increased to provide the required processing temperature.
Agitating retorts are systems designed to provide mechanical
rotation of the cans during the processing cycle to improve
heat transfer, which reduces processing time. The time re –
duction results in improved efficiency (increased product
throughput) and product quality (less heating time). Actual
processing time in either type of retort system is counted
only after the retort reaches the specified operating tempera-
ture. However, microbial lethality actually begins as the water
temperature approaches the operating temperature and con-
tinues for some time after the heating cycle ends and the
Cleaning and Washing
Size Grading
Preheating
Peeling
Inspecting
Cutting
Filling and Brining
Sealing
Retort heating
(121°C, 25–35 min depending on can size)
Cooling
(to below 50°C and ambient conditions)
Casing and Warehousing
FIGURE 18.7 Unit operations in potato canning.
Source: Mark A. Uebersax, Ph.D., Muhammad Siddiq, Ph.D.,
Carl P. Borchgrevink, Ph.D.
0
180
190
200
210
220
230
240
come
up
process cooling
vent
RETORT
TEMP.
PRODUCT
TEMP.
250
(°F)
10 20 30
TIME (MIN.)
40 50 60
FIGURE 18.8 Temperature profile and general
phases of the thermal process.
Note: The phases termed come‐up time, process time, and cooling
time for the retort versus the lagging product temperature (center
of the can).
Source: Mark A. Uebersax.
c18.indd 373 1/14/2016 9:35:56 PM
374 CULINOLOGY®
temperature begins to drop. Thus portions of the come‐up
time (heating phase) and come‐down time (cooling phase) are
built into the overall process calculations. Steam is supplied
continuously until the processing time is completed, at
which time it is shut off and the pressure is reduced. Cans are
then gradually cooled with cold water to approximately 100°F
(38°C), labeled, and cased.
Continuous retort systems—These systems are designed as hor-
izontal cylindrical tunnels with can‐carrying tracks forming a
spiral from one end to the other along the inside surface of the
cylinder. They provide agitation as the cans move down the
tunnel, twisting and turning in the tracks; this produces forced
convection currents through the product, thereby improving
heat transfer to the cold spot. Continuous systems eliminate
the time and energy lost in the starting and stopping required
for loading and unloading of batch systems.
Hydrostatic cookers—These use large vertical columns of
water called barometric legs that enable continuous entrance
and exit of cans into and out of the high‐pressure steam
chamber. The legs are generally greater than 35 feet high and,
along with the steam chamber, project vertically through the
processing plant roof, thus conserving plant floor space. Cans
are placed horizontally on a continuous chain that ascends
and descends through the legs and is exposed to the process-
ing steam chamber maintained at the desired temperature.
Process time is set based on chain speed. The legs enable
controlled, continuous preheating and cooling of cans, thus
conserving energy and improving overall efficiency. The
hydrostatic cooker is most suitable for large production runs
of identical products.
Glass and flexible packaging containers—The strength and
integrity of the double seam and the rigidity of the can enable
retort processing under direct steam pressure. Glass jars and
flexible pouch packaging, in comparison, require overriding
external pressure to ensure seal integrity. Ultimately, the
package seam integrity depends on the internal vacuum that
forms after processing as the product cools to room tempera-
ture. This is particularly important for glass, which relies on
the internal vacuum to secure the cap or lid, and flexible
containers, which lack the rigidity of metal cans. Pressure
differentials and uniformity of heating must be maintained to
assure a compliant sterilization process without losing pack-
age integrity. Generally, the containers are submerged in
water that is the same temperature as the filled product; then
the pressure vessel is closed and overriding air pressure is
applied to the water. This assures that the lids and seals
remain intact. At the completion of the specified heating
cycle, the superheated water is gradually replaced with
cooling water under stable pressure. This requires integrated
automatic control of both pressure and temperature and is
critical to a successful process. A momentary loss of over-
riding pressure results in dislodged lids or torn seals, and
even subtle changes may result in a leaky seam or cap that
allows contamination. Glass is particularly susceptible to
thermal shock and breakage, so temperature changes must
be carefully monitored and controlled.13
Aseptic Processing and Packaging
This innovative and advanced technology revolutionized clas-
sical thermal processing. In 1961, the first aseptic processing
facility (for milk) was started in Switzerland; the Tetra Pak
Company was the pioneer in commercial aseptic processing
in Europe and Asia. In the United States, this technology did
not become popular until the 1980s. Aseptically packaged
products are shelf‐stable and safe at room temperature. The
major difference between conventional and aseptic process-
ing is that the food product is sterilized separately, then filled
and sealed in a sterile container under sterile conditions.
Aseptic systems were commercially developed to provide
continuous thermal processing of heat‐sensitive products
such as fruit juices, puddings, milk, and specially formulated
dairy products.14 Products are processed through high‐
temperature, short‐time (HTST) heat exchangers, which offer
protection to heat‐sensitive quality attributes of foods such
as flavor, color, texture, and heat‐labile nutrients. High‐
par ticulate products (stews, sliced fruits, pasta in sauce),
especially with large piece size, are generally not suitable for
aseptic processing. This is because product is rapidly pumped
Commercial Sterilization of Foods
(HTST Processed and Cooled)
Sterilization of Containers
(Steam or Hydrogen Peroxide)
Sterilie Packaging Environment (Sterile
Food in Sterile Containers)
Shelf-stable, High-quality
Processed Products
FIGURE 18.9 Principles of aseptic processing and packaging.
Source: Mark A. Uebersax, Ph.D., Muhammad Siddiq, Ph.D., Carl P. Borchgrevink, Ph.D.
c18.indd 374 1/14/2016 9:35:56 PM
CHAPTER 18 Culinology® Applications in Food Processing 375
through the continuous heat exchanger, which is designed for
uniform heat transfer in a homogeneous product. Particulates
tend to disintegrate under this type of process.
Product and process requirements for aseptic systems
include:
Uniform composition of raw product—Generally, fluids or
pumpable pastes are most suitable. Typically, homoge-
nizers are used to provide a homogeneous mix.
Controlled flow rate of product—Use of a timing pump to
control residence time of product in the heat exchanger
is required for proper processing (the homogenizer
pump provides controlled flow rate).
High‐capacity heat exchanger—HTST heating followed
by cooling.
Coordinated delivery of sterilized packaging to match the
arrival of cooled product.
Sterile filling and sealing chamber to prevent recontamina-
tion of sterilized product.
The HTST systems provide rapid heat transfer at relatively
high temperatures (UHT) that range to greater than 300°F
(149°C) and expose the product to only a few minutes of heat-
ing, compared to what is typically provided in conventional
canning systems (about 250°F [121°C] for 90 minutes or
more).
When thermal processing is used to sterilize food, bacte-
rial cells and spores are inactivated at an exponential or loga-
rithmic rate (that is, the rate of destruction dramatically
increases as temperature increases). However, chemical reac-
tions in foods that lead to loss of sensory qualities generally
progress at a slower, linear or arithmetic rate (exhibiting only
incremental degradation). Thus, food safety objectives
(microorganism destruction) are met much faster, before
quality and nutrients have a chance to deteriorate. Because of
this, efforts are made to heat products as rapidly as possible,
to as high a temperature as practical, to hold them at that
temperature for a minimum period, and to cool them quickly
(as with HTST and UHT processing). This inactivates the
bacterial population while minimizing thermal degradation
of the product.
Aseptic processing is particularly successful in dairy foods
(evaporated and regular milk, sour cream, coffee creamers,
and dips), liquid egg products, puddings and gelatin desserts,
baby foods, soups, sauces, juice boxes, tomato puree and
paste, and fruit purees. There are essentially no container size
limitations with aseptic processing. Commonly, products are
packaged in containers ranging from half‐ounce coffee
creamers to juice‐box laminated cartons to 55‐gallon drums.
Metalized flexible packaging, broadly termed bag‐in‐box, is
ideal for large‐scale processed foods used as ingredients in
commercial food formulations. Even the interior of a tanker
truck can be sterilized and used as an aseptic package to
transport sterile liquids in bulk.
Aseptic bulk processing, an innovation often interfaced
directly with agricultural harvest and processing systems,
provides a means of bulk storage or transportation (using
sterilized storage silos, railcars, portable tankers, drums, and
bag‐in‐box or flexible pouches) before final processing. This
technique is extensively used in the citrus juice industry and
for tomato and grape purees. Essentially, it allows the grower
or processor to store large quantities of fruit or vegetable
juices, concentrates, and purees for extended periods after
harvest without refrigeration. It relieves crop overloads and
reduces waste by improving product flow during peak har-
vest season. Products can be used any time they are needed;
there is never an off season.
There are two basic types of product heating used in asep-
tic processing: direct and indirect:
Direct
Steam injection systems are the fastest method of heating
foods, but they are open systems, and heat‐labile flavors
may be lost. This method is typically used for products
where heat‐induced flavors, such as scorched or cooked
milk flavors, are not desired. Also, steam used in direct
heating of the product must be of culinary quality (appro-
priate for food contact). Typical products treated with
steam injection include baby foods and cereals.
100
80
60
40
20
10
1
0.5
0.1
230 240 250 260 270 280 290 300
Temperature (oF)
1% Loss
10% Loss
20% Loss
50% Loss
Microbial
Destruction
P
ro
c
e
s
s
T
im
e
(
m
in
u
te
s
)
FIGURE 18.10 Effect of time (vertical axis,
log scale) and temperature (horizontal axis) on
destruction of typical bacterial spores and
percent loss of spores.
Note: Each point on these lines represents an equivalent process.
Thus, HTST processing conditions result in microbial death with
limited quality degradation.
Source: Adapted from I. J. Pflug, C. W. Hall, and G. M. Trout,
“Aseptic Canning of Dairy Products,” Dairy Engineering, 1959.
c18.indd 375 1/14/2016 9:35:56 PM
376 CULINOLOGY®
Indirect
Plate heat exchangers are used for low‐pulp, single‐
strength juices and drinks, as high‐pulp items and con-
centrates generally stick and burn in this type of heat
exchanger. The benefits of plate heat exchangers are
that they are relatively low‐priced and have highly effi-
cient pre‐heating and pre‐cooling rates (up to 90 percent
more efficient than other methods). Typical food prod-
ucts for which plate heat exchangers are used include
milk and fruit juices.
High‐velocity, high‐pressure tubular exchangers are
favored for products that might burn, foul, or clog when
exposed to plate heat exchangers but are not viscous
enough to require or qualify for scraped plate exchangers.
The products are kept from sticking and burning by high
pressure–induced turbulence. Given the closed nature of
these systems, volatile flavor loss is prevented or reduced.
Typical food products for this type of heat exchanger
include pastes, purees, and puddings. These systems are
more expensive than plate heat exchangers but not as
expensive as scraped‐surface heat exchangers.
Scraped‐surface heat exchangers are the most expensive
type of heat exchanger and are thus used mainly for
high‐value viscous products or foods that are otherwise
heat sensitive. Typical food products heated in scraped‐
surface heat exchangers include purees, puddings, slur-
ries of fruits and vegetables, and stews. This is a closed
system, providing increased flavor retention.
Chilling and Freezing
Early food technologists recognized that low‐temperature
storage reduces the respiratory rate of freshly harvested
crops, decreases the activity of microorganisms in food, and
slows chemical and biochemical deterioration. Refrigeration
is commonly used for short‐term preservation, whereas
freezing is suitable for long‐term storage.15
Freezing Processes
The American inventor and entrepreneur Clarence F. Birdseye
II (1886–1956) is considered the founder of the modern fro-
zen food industry. During his work as a field naturalist he was
taught by the native Inuit people to fish through thick ice in
frigid temperatures. He discovered that the fish froze almost
immediately on removal from the water and, when thawed,
tasted much fresher than the frozen fish sold at that time,
which was slow‐frozen and thus lost product integrity.
Birdseye eventually developed a process for commercially
quick‐freezing fish between two refrigerated plates under
pressure, and a new industry was born.
All of the water in a food must be converted to ice before
the temperature can be reduced below freezing. Foods are
not made of pure water; therefore, they do not freeze com-
pletely at 32°F (0°C). For example, the actual freezing point of
milk and eggs is 31°F (–0.5°C); of fruits and vegetables, 30.5 to
27°F (–0.8 to 2.8°C). As the water in food begins to freeze,
salts, proteins, and other solutes in the water are concen-
trated into a smaller and smaller volume of liquid. Thus, the
concentration of solutes in the unfrozen portion increases,
lowering the freezing point of that portion. These increases in
solute concentration can damage foods by denaturation of
proteins because a high concentration of salts destabilizes
protein structure. This can lead to coagulation and reduced
water‐holding capacity, eventually resulting in diminished
product quality and functionality.
Major Factors Affecting Frozen Foods
Food quality is particularly affected by the rate of freezing.
Rapid freezing results in numerous small ice crystals that
produce less cell wall damage in fruits and vegetables, and
less cell membrane damage in meats and other non‐plant‐
based foods, than do the comparatively fewer but larger crys-
tals formed during slow freezing. Cellular damage, in which
ice crystals penetrate cell walls and membranes, results in
reduced quality, particularly texture (mouthfeel) and water‐
holding capacity (drip loss). Several factors determine how
quickly foods can be frozen:
Food composition—The components of food—fat, water, air,
protein, and so on—have different thermal properties, each
affecting how quickly a food freezes. For example, meat con-
taining a large amount of fat freezes more slowly than lean
meat (which contains comparatively more water).
Size and shape—Food processors have more control over cer-
tain freezing factors. For example, they can speed heat
removal by cutting the food into smaller pieces. That way, the
amount of surface exposed to the freezing medium is large
and the volume of each piece is small; therefore, the food
loses heat rapidly. The size and shape of a container has a
similar effect on the rate at which its contents freeze, with
larger containers taking a longer time to freeze completely, as
do stacked pallets of cased products.
Temperature differential and convective cooling—The rate of
freezing can be accelerated by increasing the difference
between the temperature of the freezing medium and the
temperature of the food. In a freezing chamber where the
medium is stationary (cold, still air), the thermostat might
have to be set at −40°F (4.4°C) instead of −20°F (–29°C) to
achieve the same rate of freezing as in chambers where the
freezing medium is moving (high‐velocity chilled air). This is
due to convection in the cold air or liquid refrigerant.
Increases in the velocity of the refrigerant speed the transfer
of heat from the food to the freezing medium.
Methods of Freezing Food
Air Freezing
Air freezing is the simplest and most common method for
freezing food. Generally, temperatures are maintained at 0 to
–40°F (–18 to –40°C). Food products are placed in a cold
room until frozen or, more commonly, pass through on
continuous conveyor belts. In air‐blast freezing systems,
c18.indd 376 1/14/2016 9:35:56 PM
CHAPTER 18 Culinology® Applications in Food Processing 377
blowers generate high‐velocity air currents (300 linear feet
per minute) in the freezing compartment to achieve rapid
freezing. Fluidized bed freezing is a type of air‐blast freezing
used to create IQF (individually quick‐frozen) foods such as
small or diced fruits, peas, shrimp, and cubed carrots. In IQF,
the product is spread about 6 inches thick on a perforated
wire mesh belt that passes through a freezing tunnel. High‐
velocity chilled air is directed upward through the food, cre-
ating lifting and wave‐like turbulence within the product.
Thus, individual pieces of food freeze rapidly (usually within
5 minutes) and efficiently. These frozen discrete pieces of
food are easy to package and use.16
Plate Freezing
Plate freezing is used for prepackaged foods. Freezer plates are
hollow, flat shelves cooled by circulating refrigerant. Packages
of food are placed between the freezer plates, which are pressed
onto the packages to speed the removal of heat from the food.
Freezing is complete in 1 to 2 hours. This method freezes pack-
aged blocks of food rapidly and is suitable for any food available
in block form, such as fish or boxed vegetables.
A variation is the rapid continuous freezing of fluid prod-
ucts or purees using a scraped‐surface heat exchanger. A
rotating scraper blade (dasher blade) moves the product
through a freezing cylinder in direct contact with its internal
cooling surface. The frozen product leaves the system as a
pumpable slush ready for immediate packaging and solid
freezing in an air‐blast freezer. Scraped‐surface heat exchang-
ers reduce product temperatures continuously and rapidly so
the frozen product contains many small ice crystals. Smooth
ice cream, orange juice concentrate, and other pumpable
purees are frozen this way.
Immersion Freezing
The immersion freezing approach puts a refrigerated coolant
in direct contact with the product or package; speed is the
main advantage. The product is immersed in or sprayed with
refrigerated solutions that are colder than ice due to their
high concentration of dissolved solids, such as salt brines,
sugar syrups, and propylene glycol. Whole packaged turkeys
are usually moved on a conveyor through the refrigerated
solution to ensure uniform freezing of the skin, giving it a
smooth, tight‐fitting appearance.
Cryogenic Freezing
In cryogenic freezing systems, low‐boiling‐point refrigerants,
placed in contact with the product or package, remove heat as
they change from liquid to vapor phase. Liquid nitrogen
(boiling point, –320°F [–196°C]) and liquid carbon dioxide
(boiling point, –109°F [–78°C]) are the most common in food
freezing. They can freeze foods very rapidly. However, because
the vapor cannot be reclaimed, the process is quite expensive.
Extremely rapid freezing enables production of high‐quality
specialty products (for example, frozen, cooked, chopped eggs
for salads) that would be unacceptable if frozen in any other
system.
Other Factors Affecting Frozen Foods
Maintenance of low (ideally 0°F [–18°C] or below) and
uniform temperature is essential for storing high‐quality
frozen foods. Temperature cycling, which causes the water
in food to partly thaw and freeze repeatedly, results in dehy-
dration (freezer burn) as the water exits the tissues and
crystallizes on the food’s surface. Lipid oxidation leading to
fat rancidity can occur at frozen temperatures, although
more slowly than at ambient or refrigerated temperatures.
This makes long‐term frozen storage of fatty foods problem-
atic. The situation is exacerbated if the food contains unsat-
urated fats along with salt or metals such as iron (commonly
found in meats containing high levels of the muscle pig-
ment myoglobin, such as beef, poultry dark meat, and fatty
fish). Salt and metals accelerate the initiation of oxidative
rancidity.
From the food safety perspective, it is impractical and
undesirable to sterilize frozen foods, as neither pathogens
nor spoilage organisms can grow at freezer temperatures.
Instead, a pragmatic balance is established between any non‐
pathogenic psychrophilic (cold‐loving) microorganisms and
pathogens that may be present. The non‐pathogenic
psychrotrophs (such as lactic acid bacteria) grow more
rapidly in thawed foods, creating acidic conditions unsuitable
for the growth of psychrophilic pathogens (such as listeria).
Furthermore, spoilage by psychrotrophs is often noticeable
through changes in appearance, odor, or taste. The disposal
of visibly spoiled, mishandled food may protect consumers
from invisible damage by pathogens. Lastly, other than ice
cream and other frozen treats, frozen foods are generally
cooked before consumption, providing a kill step for any
microorganisms that may be present.
Other factors that influence the rate of spoilage in frozen
foods include:
The level of cleanliness or contamination of the original
ingredients, finished product, and the processing facility
Freezing tunnel
Forced cold air
Fresh Frozen
IQF
FIGURE 18.11 Fluidized bed freezing.
High‐velocity cold air provides enough turbulence
to lift the product for rapid freezing of
individual food pieces—for example, peas,
diced vegetables.
Source: Mark A. Uebersax, Ph.D., Muhammad Siddiq, Ph.D.,
Carl P. Borchgrevink, Ph.D.
c18.indd 377 1/14/2016 9:35:56 PM
378 CULINOLOGY®
The duration of refrigerated storage of the product prior
to freezing
Temperatures and methods used in thawing
Thawed foods are particularly susceptible to spoilage
because as food thaws, soluble drips contain nutrients that
can be used by microorganisms for growth and the product
may be exposed to temperatures that promote rapid growth
of food spoilage organisms. Such microorganisms may be
native to the food or environmentally introduced during han-
dling and processing.
Recommended thawing procedures that limit microbial
growth generally specify moving the food from the freezer to
the refrigerator for an extended period of time (hours to
days), or, for more rapid thawing, immersing the frozen prod-
uct in cold running water for as long as necessary to achieve
the necessary level of thawing. Ideally, once thawed, products
should not be allowed to warm above refrigerator tempera-
tures before they are cooked. Microbial populations can mul-
tiply rapidly during thawing, so refreezing of thawed products
is not recommended. However, partially thawed products
still containing ice crystals or held at refrigerator tempera-
tures up to 24 hours may be refrozen safely if not mishandled,
although sensory qualities may suffer.
Previously frozen, thawed seafood products (shrimp, scal-
lops, fish fillets) are commonly available in supermarkets.
These products should not be refrozen and should be cooked
and consumed within a day or two of purchase.
Dehydration
Dehydration, the removal of water from a food product, pro-
vides the longest shelf life to foods, assuming that environ-
mental factors such as exposure to humidity and incursions
by insects and rodents are controlled. It requires the use of
external heat to vaporize and remove water from the food
solids. Two distinct processes are involved: (1) heat transfer
and (2) mass transfer. Heat transfer involves supplying
enough heat to transform the water within the food and on
its surface into steam. The quantity of heat energy needed to
vaporize water depends on the temperature and pressure at
which vaporization occurs. The latent heat of vaporization
for water (the energy required to convert water to steam) at
atmospheric pressure is about 970 BTU per pound of water at
212°F (100°C). Generally, heating involves conduction, con-
vection, and radiation processes of heat transfer, which are
highly dependent on a broad spectrum of product and proc-
ess variables (product type, solid and liquid content, piece
geometry, size, and shape) and the physical conditions of the
heating environment (temperature, air velocity, and relative
humidity).
Mass transfer involves the removal of moisture from the
surface of the food and the internal diffusion of water to the
surface of the product. These phases of the drying process are
referred to as (1) constant rate, associated with the initial
stage of rapid removal of moisture from the surface, and
(2) falling rate, associated with the slower and subsequent
internal diffusion of water to the surface for evaporation.
Time
A
B C
D
D
ry
in
g
r
a
te
FIGURE 18.12 Typical drying rate curve of
foods. (A‐B, initial accelerated rate; B‐C, constant
rate; C‐D, falling rate).
Source: Mark A. Uebersax, Ph.D., Muhammad Siddiq, Ph.D.,
Carl P. Borchgrevink, Ph.D.
The constant rate of surface drying is directly influenced
by the temperature, relative humidity, and velocity of air over
the food surface. During the falling rate, drying rate decreases
because it takes longer for water to migrate from the interior
of the food to the surface.17
Drying Systems
Sun Drying
This is an age‐old practice used for whole or cut pieces, com-
mon in most regions with arid locales. Dates, figs, tomatoes,
fish, and grapes (into raisins) are the most commonly sun‐
dried foods. The process typically takes place in areas adja-
cent to the harvest location. The drying of salt, herbs, and
spices also appears in the earliest recorded histories.
Hot‐Air Drying
During the twentieth century, advanced methods for drying
food products were developed that did not depend on the
availability of a hot, sunny climate and were much more effi-
cient. Development accelerated during the first and second
World Wars due to the need for global transport and pro-
longed shelf life of dehydrated foods to feed the troops.
Development of dehydrated vegetable products in particular
has undergone significant growth, with greatest advances in
potato, onion, pepper, and garlic products. These are sold as
individual food products or as convenient ingredients in
formulated foods.
Hot‐air drying can be accomplished using high‐velocity
hot air (140 to 185°F [60 to 85°C]) to heat and vaporize water
and to convey moisture vapors away. Hot‐air drying systems
include cabinets equipped with perforated trays, heated tun-
nels through which carts or racks of products are conveyed,
and continuous perforated belts with different heating zones.
A special fluidized bed technique, which uses high‐velocity
c18.indd 378 1/14/2016 9:35:57 PM
CHAPTER 18 Culinology® Applications in Food Processing 379
separated using cyclone (vortex) separators, with powdered
solids falling to the bottom, where they are collected.
Various configurations of hot air flow (concurrent, in
which droplets are blown down; countercurrent, in which
air enters the base of the tower and travels upward against
the falling droplets) are in commercial use. Numerous
design configurations and means of process control (prod-
uct and air temperature, regulation of flow rates and air tur-
bulence) enhance the efficiency and versatility of the
process and prevent excessive heat damage. The use of vari-
ous agglomeration techniques (particle‐to‐particle aggre-
gation) enhances rehydration properties and the production
of highly soluble “instantized” powders. Spray drying is used
to produce dairy products (milk, nonfat dry milk, and whey
solids), dehydrated eggs, dehydrated juice, and beverage
mixes (powdered juices, instant coffee, cocoa, and iced tea),
and formulated foods (instant soups, sauces, and premixes).
The systems are also standard in the manufacture of pow-
dered flavors and colors.
BELT DRYER
FIGURE 18.14 Commercial dehydration of apple slices.
Source: Mark A. Uebersax, Ph.D., Muhammad Siddiq, Ph.D., Carl P. Borchgrevink, Ph.D.
FIGURE 18.13 Principles of various drying operations.
System Principle Product Type Examples
Sun drying Radiant heat from the sun evaporates
water from food to surrounding air. This
is an age‐old method of drying foods.
Fruits (raisins, dates, figs), vegetables
(herbs, tomatoes, cereals, and
legumes), fish, meat, coffee beans
Hot-air drying (cabinet,
tunnel, continuous belt,
fluidized bed)
Stream of hot air in a closed environment
supplies heat by convection and carries
away the evaporated water.
Fruit pieces (apple, peaches, plums,
pears, apricots), particulate foods
(meats, fish, cheese)
Spray drying Special case of hot-air drying; fluids are
sprayed into the stream of hot air; dry
powder is separated mechanically.
Liquids, purées, whole eggs, egg yolk,
blood albumin, milk, flavors, colors
Vacuum drying Removal of water vapor by creation of
vacuum; heat must be supplied by
conduction or radiation (no air used).
Limited production of certain foods,
pieces, purées, liquids
Drum drying Thin layer of liquid or paste foods applied
on surfaces of hot stainless-steel drums;
dry material is scraped off the rotating rolls
at the end of a revolution.
Purées, liquids, milk, certain vegetable
juices, cranberries, bananas, oatmeal,
mashed potatoes
Freeze drying Frozen food lyophilized under vacuum. Specialty high‐value foods
Source: Mark A. Uebersax, Ph.D., Muhammad Siddiq, Ph.D., Carl P. Borchgrevink, Ph.D.
hot air to turbulently lift and propel the product, is highly
effective for drying particulate products (a similar process to
the one used to make IQF products). The movement of air
may be countercurrent (in the opposite direction) or concur-
rent (in the same direction) with respect to the product being
dried.
Spray Drying
Spray drying affords a high‐volume, economical means of
drying fluid products. The techniques are generally described
as “air drying of liquid foods” and vary in complexity and
sophistication. The fluid product is typically preheated in a
continuous heat exchanger and pumped under pressure to
the top of a tall tower or chamber that delivers high‐volume
and high‐velocity hot air ranging from 302 to 932°F (150 to
500°C). The preheated product is atomized through a nozzle
or mechanically dispersed as very small droplets. As the
droplets descend through the tower, moisture is vaporized
and removed with the exit air. The powdered product is then
c18.indd 379 1/14/2016 9:35:57 PM
380 CULINOLOGY®
Drum Drying Systems
Drum drying is a specialized technology used for making
dried powders from viscous slurries and pastes that cannot
be dried by spray dryers or other types of commercial drying
systems (tunnel dryers, box dryers, tray dryers, etc.). Drum
drying is used extensively in infant cereal production as well
as for instant dehydrated potato flakes and oatmeal.18
Generally, two hollow steel drums (drum dimensions:
1.65 meters diameter by 4 meters length; total drying
surface: 31 square meters) are positioned parallel and adja-
cent to one another, forming a trough between them. Drum
clearance is adjustable, with the open gap referred to as the
nip. The drums are heated internally by pressurized steam
(steam pressure ~115 to 135 psi; drum surface temperature
~464°F [240°C]), and slowly rotated in opposite directions
away from the trough. Preheated slurry is fed into the
trough and, as the drums rotate, a thin film of the hot slurry
is spread over the drum surface. Direct‐conduction heat
transfer provides rapid and effective dehydration. Drum
temperature and residence time (248 to 338°F [120 to 170°C]
for 20 seconds to 3 minutes) is easily controlled and provides
for rapid evaporation of water. The dehydrated product on
the drum surface is scraped off as flakes using an angled
blade called a doctor blade. The large, sheeted flakes are
then size‐reduced to smaller uniform flakes (instant
oatmeal) or finely milled to powders (instant potatoes and
baby cereals).
Drum drying is one of the most efficient and economical
drying methods; however, product deterioration (color, fla-
vor, texture) is frequently greater than that caused by less
severe methods.
Freeze‐Drying Systems
Freeze drying, or lyophilization, is a relatively expensive
process used for certain high‐value specialty foods. A major
advantage of freeze drying is the retention of product
structure and texture as well as volatile flavor compounds.
Rapid freezing prevents the cellular wall and membrane
FIGURE 18.15 The principal components of a spray‐drying system.
Source: GEA Niro.
c18.indd 380 1/14/2016 9:36:01 PM
CHAPTER 18 Culinology® Applications in Food Processing 381
breakage caused by large ice crystal formation. The freeze‐
drying process is used to prepare meats (beef, pork, and
chicken), seafood (shrimp, crab), high‐value fruit and vegeta-
ble products (berries for ready‐to‐eat cereals), precooked
legumes (microwavable bean ingredients), and complex
ingredient blends (stock bases, herbs, mushrooms). Its great-
est commercial success is in the production of instant cof-
fees and complete dehydrated meals (as for camping).
Freeze‐dried astronaut ice cream, developed for the National
Aeronautics and Space Administration (NASA) in 1968, is
still available at camping stores. Quality attributes (color, fla-
vor, texture, and rehydration potential) are well preserved,
and the resulting products are extremely lightweight.
Under the proper conditions of temperature and vacuum,
water reaches the so‐called triple point where the phases of
solid, liquid, and vapor converge and water vapor can be
sublimed directly from ice without passing through the
aqueous phase. The process requires two stages; the first
involves the initial freezing of the food, and the second pro-
vides the temperature and negative pressure conditions nec-
essary to effect direct moisture removal. Very low final
product moisture content (<1.0 percent) is achievable.
Typically, freeze drying is a batch operation in which the pre‐
frozen product is placed on sensitive heating plates within a
vacuum chamber. The ice temperature (controlled by the
plates) and vacuum pressure are independently adjusted to
conditions that directly vaporize the moisture. The moisture
vapor is removed on condensers maintained outside of the
chamber. Due to the relatively slow rate of moisture removal
and the limited production volumes capable in a batch oper-
ation, operating costs are quite high.
Concentrated and
Condensed Foods
Fluid foods are commonly concentrated before further dehy-
dration or direct use. Water is more economically removed
in highly efficient evaporators than in final dehydration
equipment. This process can range from simple atmospheric
open‐kettle boiling to highly engineered systems where heat
exchangers provide sufficient thermal energy to vaporize
Dry
Milk
Powder
Product
Infeed
Knife
Blade
Water VaporWW
Screw
Conveyors
FIGURE 18.16 Drum drying.
Source: Mark A. Uebersax, Ph.D., Muhammad Siddiq, Ph.D., Carl P. Borchgrevink, Ph.D.
Temperature
gaseous phase
liquid
phase
solid phase
triple point
P
re
s
s
u
re
FIGURE 18.17 Freeze drying. An illustration of
the triple point of water, showing phase changes
under different pressure and temperature conditions
(ice solid phase to gaseous water vapor phase).
Source: Mark A. Uebersax, Ph.D., Muhammad Siddiq, Ph.D.,
Carl P. Borchgrevink, Ph.D.
c18.indd 381 1/14/2016 9:36:02 PM
382 CULINOLOGY®
the water. As water is vaporized, the product is concentrated
or condensed and becomes more viscous. Condensed prod-
ucts, possessing increased solids content, have the advan-
tage of convenience of handling (smaller containers),
reduction in solid waste disposal (packaging), and lower‐
cost transportation and distribution. Condensed products
include canned soups (reconstituted with water before
reheating), tomato paste (canned or aseptically packed in
flexible film pouches), frozen juice concentrates, canned
evaporated milk, and sweetened condensed milk. The fol-
lowing methods of concentration are commonly used:
Flash evaporators—These rapidly heat the product by
contact with 302°F (150°C) steam injected directly into
the food. The boiling mixture then enters a separator
vessel in which the concentrated food is drawn off at
the bottom and the steam plus water vapor is evacu-
ated through a separate outlet. Essence recovery
systems (liquid nitrogen or carbon dioxide condensers)
may be used to trap highly volatile flavor compounds
that are recovered from the vapor fraction and added
back to the final product. In the coffee business, the
aromatic vapor given off by freshly roasted coffee beans
during the grinding process (called “grinder gas”) is
captured using essence recovery and then used to dose
jars of instant coffee to provide the promise of rich
flavor when the jar is opened initially and the aroma
wafts out.
Thin‐film evaporators—These systems use high‐tempera-
ture, short‐time (HTST) heat exchange principles to
achieve rapid water removal with minimal product qual-
ity damage. Equipment typically incorporates a rotating
swept‐surface paddle that distributes a thin layer of
product inside an externally heated cylinder. Water is
readily flashed from the thin food layer, and the concen-
trated food is removed by mechanical action of the pad-
dle. Thus, high volumes of fluid products (milk, fruit
juices, and purees) can be concentrated in a continuous
process.
Vacuum evaporators (vacuum pans)—Heat‐sensitive foods
are most commonly concentrated in low‐temperature
vacuum evaporators. Typically, several vessels are con-
nected in series to create a multiple‐effect vacuum
evaporator. Such a sequence enables efficient evapora-
tion yielding high‐quality concentrates (a single‐effect
evaporator requires more steam per pound of water
removed than the multiple‐effect evaporator—1:1 versus
1:4, respectively). As the partially concentrated product
proceeds through the series of evaporators, it is heated
under successively increasing levels of vacuum. Because
the product is being heated under vacuum, the boiling
point is reduced. Thus, the product is efficiently heated
and water is removed at decreasing temperatures, con-
serving both quality and energy. Multiple‐effect vacuum
evaporators with essence recovery systems are most
commonly employed in the citrus, grape, and tomato
juice industries.
Advanced ultrafiltration or reverse‐osmosis tech-
nologies—A solution is defined as a liquid mixture in
which the minor component (the solute) is uniformly
dissolved within the major component (the solvent). For
example, in a sugar solution, the water is the solvent and
the sugar is the solute. Osmosis is the natural tendency
of the water portion of a dilute solution on one side of
a semipermeable membrane to pass freely through
the membrane into a solution on the other side of the
membrane where the solute concentration is higher,
diluting it, until the concentrations of the solutions are
equal on both sides of the membrane. The solute mole-
cules are too large to pass through the membrane
and are trapped on either side. Only the water—the
solvent—can pass through the membrane. The pressure
exerted by the molecules of the solvent on the mem-
brane as they pass through is called osmotic pressure.
Osmotic pressure is the energy driving osmosis and is
important for living organisms because it allows
water and nutrients dissolved in water to pass through
cell membranes.
Reverse osmosis is a technique in which pressure is
applied greater than the osmotic pressure of the solution, to
force liquid through the semipermeable membrane in the
PRODUCT
759
(175°)
165°F
115°F
809
WATER AT 60°F
33.3 GPN
STEAM AT 6.71 PSIA
459 POUNDS/HOUR
LIVE STEAM
HEATER “L”
125
C
O
N
D
E
N
S
E
R
S
E
P
A
R
A
T
O
R
T
U
B
E
C
H
E
S
T
@
@
45%
40°FFEED
FLASH
50
FIGURE 18.18 Single‐effect evaporator.
Source: Mark A. Uebersax, Ph.D., Muhammad Siddiq, Ph.D.,
Carl P. Borchgrevink, Ph.D.
c18.indd 382 1/14/2016 9:36:03 PM
CHAPTER 18 Culinology® Applications in Food Processing 383
opposite direction to that in normal osmosis. Concentrated
syrups, juices, including grape juice used in the produc-
tion of wine, and whey proteins are commonly processed
in this manner. The benefit of this system is that it does
not require heating of the fluids, thereby avoiding heat‐
influenced changes (loss of volatiles and vitamins, devel-
opment of cooked flavors or colors).
Osmotic Preserves
(Jams, Jelly, Preserves)
Processing of fruit with appropriate levels of pectin, acid, and
sugar is a time‐honored tradition that produces delicate and
flavorful jams and jellies. These products are classified as
intermediate‐moisture foods with low water activity (a
w
),
which inhibits bacterial growth and limits growth of yeast
and molds that may cause spoilage in the final product, par-
ticularly after the container is opened. The gel qualities are
highly dependent on the ratio of ingredients in the formula
and the processing and packaging conditions employed.
Standard jellies are made with blends of fruit juice and sugar
(45 parts fruit juice and 55 parts sugar) with 2 to 3 percent
added pectin (depending on the fruit type), 0.3 to 0.5 percent
citric acid, and a pH typically around 3.3. These contents are
concentrated to at least 65 percent soluble solids (65° Brix) by
cooking in open kettles or, more commonly, using vacuum
kettles. The cooked (concentrated) product is hot‐filled
directly into the final package and may also be subject to
post‐packaging pasteurization. The contents then undergo
gel formation upon cooling. Gelled fruit products are defined
by federal standards of identity (CFR Title 21, Part 150,
Subpart B: Requirements for Specific Standardized Fruit
Butters, Jellies, Preserves, and Related Products).
The manufacturing of gelled fruit products varies from
small‐scale conventional kitchen batch operations to large‐
scale commercial enterprises employing high‐capacity vacuum
evaporators and high‐speed filling and sealing machinery.
Fruit and Juice Preparation
High‐quality mature produce, free from mold and visual
spoilage, is essential to producing high‐quality finished prod-
ucts. The level of ripeness directly influences the content of
native pectin and the sugar‐to‐acid ratio in the fruit. Slightly
underripe fruit contains more pectin than ripe fruit. Overripe
fruit commonly possess deficiencies in sugars, acidity, and
native pectin, and thus formulas must be adjusted to provide
the necessary ingredients for optimum performance.
Fruit juices can be obtained from combinations of fresh,
frozen, and canned fruit. Fresh fruits are typically heated to
Feed
Membrane
Permeate
Concentrate or
Retentate
FIGURE 18.19 Conceptual diagram of a typical membrane filtration system.
Source: Mark A. Uebersax, Ph.D., Muhammad Siddiq, Ph.D., Carl P. Borchgrevink, Ph.D.
RigidityContinuity
Pectin (%)
Loose Firm
0.5 1.0 1.5
Acid (pH)
JELLY STRENGTH
Tough Weak
2.7 3.1 3.5
Sugar (°Brix)
62 66 70
Weak Crystals
FIGURE 18.20 The relationship of pectin, acid, and sugar to gel characteristics.
Source: Desorsier (1963).
c18.indd 383 1/14/2016 9:36:04 PM
384 CULINOLOGY®
inactivate enzymes, solubilize pectin, and improve overall
yield, and are subsequently milled to a puree to maximize
juice extraction. This puree may be used as is for preserves,
jams, and fruit butters. Alternatively, for jellies, heated pulp
can be filtered by rack and cloth pressing or, more commonly,
by using continuous filtration equipment for juice extraction.
Juice clarification, using cellulose‐based or bentonite clay
filters, may be required to remove suspended solids from the
juice to maximize jelly clarity.
Most fruits used to make jams and jellies do not contain suf-
ficient pectin to form a firm gel; therefore, purified commercial
pectins are usually added, both at home and at the production
FIGURE 18.21 Characteristics of gelled fruit products.
Product Fruit Characteristic Formulation Conditions Final Soluble Solids (%) Examples
Jelly Whole or clarified
juice
Not less than 45 parts
by weight of fruit juice
of standard composition
as defined by USDA
and 55 parts of sugar
Not less than 65% Grape, apple, apricot
Jam and
preserves
Jam: Highly
comminuted (finely
chopped) fruit
Preserves: Incorporate
pieces of whole intact
fruit
Not less than 45 parts
by weight of fruit to
each 55 parts by
weight of sugar.
Not less than 65% or
68%, depending on the
fruit used
65%: apple, apricot,
plum, fig, guava,
nectarine, peach,
pear, quince, currant,
cranberry, and
gooseberry
68%: berries, cherry,
grape, citrus, pineapple,
rhubarb, tomato, and
crabapple
Marmalades Skins and peels Same as preserves Not less than 65% or
68%, depending on the
fruit used
English bitter, sweet
orange, lemon
Fruit butters 5 parts fruits + 2 parts
sugar
Not less than 43%
soluble solids
Apple and pear
Source: Mark A. Uebersax, Ph.D., Muhammad Siddiq, Ph.D., Carl P. Borchgrevink, Ph.D.
Jelly Jam/Preserves/Marmalade
Fruit Fruit
Juice extraction/clarification Purée
Water and sugar Water and sugar
Pectin (0.5%) Pectin (0.25%)
Evaporate (65° Brix) Addition of fruit/pieces/peels
Adjust pH to 2.9–3.2
(citric acid)
Concentrate by heating
(68° Brix)
Package Adjust pH to 3.3 (citric acid)
Package
FIGURE 18.22 The process sequence of jelly and jam, preserves, and marmalade manufacture.
Source: Mark A. Uebersax, Ph.D., Muhammad Siddiq, Ph.D., Carl P. Borchgrevink, Ph.D.
c18.indd 384 1/14/2016 9:36:04 PM
CHAPTER 18 Culinology® Applications in Food Processing 385
facility, to ensure proper gel formation. Several types of pectin
are available, depending on the end product desired:
Rapid‐set pectin—This is the typical choice for most jams
and marmalades. It is especially useful in jams contain-
ing fruit chunks or whole berries, which may have a
tendency to float to the top of the jar ; the faster the jam
gels, the less opportunity there is for float, and the bet-
ter the uniformity of fruit distribution within the jar.
Slow‐set pectin—Forms gels more slowly, which prevents
premature gelation and facilitates filling of large-scale
jam and jelly batches. Jam manufacturers often use cus-
tom blends using combinations of rapid set and slow set
pectins to take advantage of the benefits of both.
Stabilizing pectin—Used in acidic protein products such as
yogurts, whey, and soy drinks that need a stabilizing agent.
Low methyl ester and amidated pectin—are used for low-
sugar/no-sugar-added jams. Unlike regular pectins, gela-
tion does not depend on high levels sugar and acid.
Instead, calcium ions (usually in the form of added calcium
citrate) are necessary for a gel to form. Because the sugar
concentration (“Brix”) is so low, these products also require
the addition of a preservative in order to prevent the
growth of spoilage organisms. In addition, because the
amount of sugar is less than that mandated by the federal
standard of identity for jams and jellies, these products
have to be labeled as “fruit spreads.” Sauces and marinades,
savory or sweet, often use this thickening agent as well.
Cooking, Processing, and Packaging
Cooking processes are used to facilitate dispersion and solu-
bility of the ingredients and to concentrate the sugar by the
removal of water. The numerous chemical reactions, includ-
ing partial hydrolysis of pectin, hydrolysis of sucrose into
glucose and fructose forming invert sugar, coagulation of
proteins, and the development or degradation of flavor pro-
files, are accelerated at high temperature. Atmospheric boil-
ing at 212°F (100°C) (open kettle) results in evaporation of
water to the desired end point but dramatically decreases
the content of volatile flavor compounds (alcohols, alde-
hydes, and ketones). The jellying point temperature indi-
cates the end point of cooking; it is typically 7 to 8°F (–14 to
–13°C) above the boiling point of water—that is, 219 to 220°F
(104 to 104.4°C) at sea level. A refractometer is normally
used to confirm the actual soluble solids content (Brix).
Vacuum kettle evaporators can achieve a boiling point as
low as 140°F (60°C). Concentration under vacuum results in
less temperature abuse, with boiling and evaporation occur-
ring efficiently at much lower temperatures. This greatly
enhances the retention of color, flavor, shape, and texture of
fruit pieces, and retains a great deal more of the volatile flavor
compounds, which would otherwise “flash off ” into the
atmosphere.
To improve the integrity of fruit in preserves, a portion of
the juice can first be highly concentrated before the addition
of fruit. The fruit and high‐solids juice fraction readily equili-
brate, and the combined mix is subsequently evaporated to
the proper end point with minimal fruit damage. Because the
juice has already been concentrated, less cooking is required
to achieve the required Brix. Acids may be added at the end of
the cooking, immediately before filling; this minimizes acid‐
catalyzed hydrolysis of pectin, which results in reduced gel-
ling capacity and “soft sets.”
The properly concentrated fruit and sugar mix may be
hot‐filled (greater than 180°F [82°C]) directly into sanitized
containers and sealed. No additional processing is typically
required; however, relatively slow and uniform cooling rates
are important to ensure proper gel set. Large commercial
containers may require special filling and chilling to enable
proper gelling without the defect of “soft centers” resulting
from slow cooling at the center of the containers.
Formulation and Function of
Ingredients in a Pectin Gel
Developing pectin gel formulations is a highly technical sci-
ence combined with an artisan’s touch (Culinology® in
action!). The quality of the final product depends on the
proper blend of components contributing to gel formation
and the proper mixing, heating, cooling, and packaging of
the product. The functional ingredients contributing to the
gel include:
Pectin—This long‐chain hygroscopic polysaccharide pro-
vides structural support to plant cell walls and helps bind
cells together. It is found in highest concentration in citrus
fruit peels and apple pomace (press‐cake left behind in the
production of apple cider), from which it is commercially
extracted. When heated in an aqueous medium, such as
fruit juice adjusted to contain the optimal amounts of sugar
and acid, pectin develops an aligned lattice structure, turn-
ing the juice into a gel.
Acid—Native fruit acidity and added organic acids (citric
acid, malic acid) are used to reduce pH to optimum levels
(generally about pH 3.0) for gel formation.
Sugar—Sucrose serves as a dehydrating agent, which
facilitates greater hydrogen‐bonding and contributes to
the yield, flavor, color, texture, and preservation properties
(via reduced a
w
).
Water—This is necessary for dissolving and suspending
components of the gel.
Gel formation occurs under a definite equilibrium range
within a system of pectin, acid, sugar, and water and is
affected by temperature and pH. Solubilized pectin is pre-
cipitated by the added sugar, which disturbs the equilibrium
between the water and pectin. The pectin precipitates as a
hydrated colloid that forms a network of fibrils throughout the
mass, binding the sugar syrup into a gel matrix. Acid helps
set the gel.
c18.indd 385 1/14/2016 9:36:04 PM
386 CULINOLOGY®
Food Extrusion
Extrusion technology is widely used for processing large vol-
umes of diverse food products, including breakfast cereals,
snack foods, pasta, puffed products, and food ingredients
such as textured vegetable protein (TVP). Extrusion employs
a continuous mixing, kneading, and shaping process with
subsequent expulsion through a forming die to create a
cooked and shaped food product.19 High‐temperature extru-
sion is used extensively by many sectors of the food industry
to produce food products with distinctive texture and flavor
characteristics. Desirable end‐product properties are ob tained
by varying the processing conditions as well as the composi-
tion of the raw material.20 Moistened starchy and protein‐
based foods are cooked and worked into viscous, plastic
doughs; these are extruded and subsequently deep fried,
toasted, or dehydrated (as in expanded snack foods—cheese
puffs, curls, formed chips).
Extrusion cooking has multiple effects on food, including
gelatinization of starch, denaturation of proteins, inactiva-
tion of raw food enzymes, destruction of naturally occurring
toxic substances, and reduction of microbial counts. Other
significant structural changes in proteins and starches occur
that may be used to generate texturized meat analog
products. The extrusion process can also eliminate anti‐
nutritional factors present naturally in certain foods, such as
lectins in beans or legumes, which cause food poisoning if
inadequately cooked.
Extrusion dies are designed to create specialized shapes—
for example, low‐density puffed balls or sticks, or high‐
density texturized strands. Extruders range from simple cold‐
temperature pasta presses to highly engineered screw types
(single‐screw or twin‐screw), which employ high pressure
and temperature. Shear force and steam simultaneously mix,
cook, and expel the raw ingredients through dies into finished
products of various sizes, shapes, and textures. The process is
energy efficient and environmentally friendly; unlike many
other processing techniques, little solid or liquid waste is pro-
duced. The diversity of extruded foods and food ingredients
is evident from the broad base of raw feed stocks employed.
Rotating
knife
(a)
(b)
Die
Flavor/Color
Water/Steam
Heating/Cooling jacket
Raw materials
Motor
COOLING/HEATING/CONVEYING
Cooking/Melting
Amorphosizing
Texturizing
KNEADING
ZONE
(Dough Like Mass)
Kneading
Reacting
Compression/Feeding
FEED ZONE
(Raw Material and
Surface Moisture)
FINAL COOKING
ZONE
(Visco-Amorphic Mass)
Conveying zone
Rotating screws
Cooking zone
(shear)
High pressure
zone
Viscous flow
Gelatinization
Denaturation
Plastification
Mixing zone
Barrel
section
S
H
A
P
I
N
G
FIGURE 18.23 Cross‐sectional views of the single‐screw extrusion cooker system (a) and a simple
extruder barrel/expander (b).
M. N. Riaz, “Introductions to Extruders and Their Principles,” in M. N. Riaz (ed.), Extruders in Food Applications (Boca Raton, FL: CRC Press, 2000).
c18.indd 386 1/14/2016 9:36:04 PM
CHAPTER 18 Culinology® Applications in Food Processing 387
These range from doughs or pastes made from whole cereal
grains or oilseeds (for example, wheat, corn, soy) to special-
ized formulations of protein, starch, or fiber fractions.
Specialized Processing
Technologies
Cook‐Chill and Sous Vide
In the United States, cook‐chill and sous vide are typically
seen as distinct processes; in Europe, the terms are often
used interchangeably.21 Sous vide literally translates as “under
vacuum” or “vacuum packed,” and cook‐chill products are
not always packed under vacuum. In the United States, cook‐
chill is a batch preparation approach, while sous vide typically
involves individual servings. Lastly, while cook‐chill products
may be cooked using any cooking or thermal processing
method, sous vide is always cooked in a temperature‐
controlled water bath.
The cook‐chill process may be either cold fill or hot fill. In
cold fill, the container, such as a food‐grade pouch, is filled
with raw foods, sealed, and subsequently cooked to a desired
temperature for the required length of time. This is similar to
the sous vide process. In hot fill, the food is cooked in a tradi-
tional fashion and packaged immediately upon completion.
(A third variation, which involves cooking to desired done-
ness, rapid cooling, and then bagging, is also possible.)
Sous vide is a cold‐fill process. The raw food is always vac-
uum‐packed in pouches and always cooked in water baths.
The food may be cooked immediately after vacuum packaging,
then rapidly chilled and held for later reheating and service,
or the raw vacuum‐packed food may be held refrigerated (or
frozen) until needed and then cooked to order. A primary ben-
efit is that sous vide permits even delicate foods to be mass pro-
duced using closely controlled temperatures (with tolerances
as close as ±1°F [~0.5°C] in water baths), which leads to better
quality and increased yield.22 The increase in yield can be dra-
matic; studies point to sous vide foie gras (literally: “fatty liver”;
this French delicacy is typically made from duck liver)
production that reduced cooking loss from 50 percent to 5 per-
cent!23 While this example is extreme, the lower‐temperature,
controlled cooking of sous vide production does lead to marked
decreases in shrinkage and cooking loss. Also, water‐soluble
flavor components, nutrients, and volatile aroma chemicals
stay with the food inside the cooking pouch rather than
leaching out into the cooking water or evaporating into the air.
In addition, cooking in a temperature‐controlled water bath
eliminates overcooking entirely, as the temperature of the food
can never exceed the temperature of the water bath.
One shortcoming of this technique is that cook time tends
to be quite long. Because the final cooked temperature of the
product is the same as the hot water bath, it takes quite a
long time for heat transfer to take place as the raw product
slowly equilibrates to the water bath temperature.
Another shortcoming of sous vide cooking is that foods
cooked in the high‐moisture environment of a sealed pouch
can never develop the roasted flavors or aromas created by
Maillard browning (which requires dry‐heat cooking meth-
ods). If the food in question must be browned, seared, or oth-
erwise marked, or crust formation is desired, this can be done
after cooking is completed and the bag is removed, immedi-
ately before service. Juices can be reserved for sauce prepara-
tion. Given the single‐serve size of most sous vide products,
cooking is precise. The desired degree of doneness (and color
in the case of cooked‐rare red meat products) permeates the
food from the edge to the center.
Cook‐chill has been around for a long time, but previously
it was called batch cooking, where foods were prepared in
large batches and subsequently chilled, then portioned,
wrapped or bagged, and held for later use. Compared to sous
vide, cook‐chill provides greater process flexibility in that
almost any heat source or cooking method can be used.
However, hot‐fill applications experience typical shrinkage
and yield loss from cooking before bagging.
The greatest product yields are found in cold‐fill applica-
tions. This is commonly done for whole muscle meats,
which are bagged with the desired seasonings and vacuum‐
sealed before cooking. Liquid foods, such as soups, sauces,
and stews, are best suited for hot fill. Typically, these foods
are cooked in large kettles with automatic agitators and
then pumped into bags, evacuating all excess air before seal-
ing. Key to hot‐fill applications is a high enough filling tem-
perature (a minimum of 180°F [82°C]) to maintain sanitary
conditions inside the pouch. Once packaged, the food is rap-
idly chilled to prevent the quality deterioration that comes
from overcooking. The rapid decrease in temperature is
designed so the food moves quickly through and below the
140 to 40°F (60 to 5°C) danger zone to minimize the growth
of spoilage and pathogenic microorganisms. The rapid cool-
ing is often done in cold‐water tumble chillers or cold‐air
blast chillers.
According to the FDA 2009 Food Code, cook‐chill is one of
several food processing methods defined as reduced‐oxygen
packaging (ROP). ROP foods are packaged in environments
that contain little or no oxygen. The other methods are
controlled‐atmosphere packaging (CAP), modified‐atmosphere
packaging (MAP), sous vide, and vacuum packaging. If the food
in the ROP environment is one the FDA recognizes as poten-
tially hazardous, (pH above 4.6, high moisture, high protein con-
tent), a Hazard Analysis and Critical Control Point (HACCP)
plan must be developed. The benefits of ROP include extended
shelf life, an anaerobic or near‐anaerobic environment that pre-
vents or slows the growth of aerobic spoilage organisms (such
as pseudomonads, yeast, and molds), slowed oxidative rancid-
ity and other degradative changes initiated by exposure to
oxygen, and retention of the natural color of foods (especially
meats).
However, due to the anaerobic or near‐anaerobic environ-
ment created inside the package, there is always the potential
c18.indd 387 1/14/2016 9:36:04 PM
388 CULINOLOGY®
for outgrowth of botulism spores and listeria. Therefore,
special care must be taken to process, distribute, and store
ROP foods with great attention to detail. All foods that fall
into the potentially hazardous foods (PHF) category that are
processed using one of the ROP methods must be stored
under refrigeration (or freezing) up to the point of final cook-
ing or reheating and service. In addition, they cannot be sold
for retail use, as there is no way to control how consumers
handle (or mishandle) them. Knowledge about the impor-
tance of maintaining proper temperature levels in home
refrigerators and freezers is not widespread.
Cook‐chill and sous vide foods are also referred to as mini-
mally processed refrigerated (MPR) foods.24 ROP and MPR
approaches to food processing increase food safety concerns,
as these foods are particularly susceptible to the temperature
abuse that may occur anywhere along the ingredient sourc-
ing > food manufacturing > end user continuum. In particu-
lar, consumers often cannot or do not maintain adequate
temperature control of potentially hazardous foods (PHF), in
part because they do not recognize them as being potentially
hazardous. For ROP and MPR PHFs, HACCP plans and
detailed labels are not sufficient to guarantee product safety
in the home. These foods must be designed using additional
safety treatments or hurdle technology and predictive micro-
biology; however, an in‐depth discussion of this critical area
is beyond the scope of this chapter.25
Irradiation
The use of ionizing radiation for food preservation has an
extensive history of scientific study. Prepackaged or bulk
foods are exposed to ionizing radiation (gamma‐rays, x‐rays,
or beams of electrons) in specially designed shielded
chambers for a designated time. The objective is to control
biological processes in produce (such as respiration and
sprouting), kill insects, and reduce the number of pathogens
FIGURE 18.24 Approaches to reduced‐oxygen packaging (ROP).
Cook‐chill A process that uses a plastic bag filled with hot cooked food from which air has been
expelled and that is closed with a plastic or metal crimp or heat sealed.
Controlled Atmosphere
Packaging (CAP)
An active system that continuously maintains the desired atmosphere within a package
throughout the shelf life of a product by the use of agents to bind or scavenge oxygen or a
sachet containing compounds to emit a gas.
Packaging of a product in a modified atmosphere followed by maintaining subsequent
control of that atmosphere.
Modified Atmosphere
Packaging (MAP)
A process that employs a gas flushing and sealing process or reduction of oxygen through
respiration of vegetables or microbial action.
Packaging of a product in an atmosphere that has had a one‐time modification of gaseous
composition so that it is different from that of air, which normally contains 78.08% nitrogen,
20.96% oxygen, and 0.03% carbon dioxide.
Sous Vide A specialized process of ROP for ingredients that require refrigeration or frozen storage
(PHF/TCS food*) until the package is thoroughly heated immediately before service.
A pasteurization/cooking step that reduces bacterial load but is not sufficient to make the
food shelf‐stable.
The process involves the following steps:
(a) Preparation of the raw materials (this step may include grilling or broiling for color of
some or all ingredients);
(b) Packaging of the product immediately before cooking, application of vacuum, and seal-
ing of the package;
(c) Pasteurization/cooking of the product using required time/temperature parameters;
(d) Rapid and monitored cooling of the product at or below 1–3°C (34–38°F) or frozen;
and
(e) Reheating of the packages 74°C (165°F) for hot holding or to any temperature for imme-
diate service before opening and service.
Vacuum Packaging Reduces the amount of air from a package and hermetically seals the package so that a
near‐perfect vacuum remains inside.
A common variation of the process is Vacuum Skin Packaging (VSP). A highly flexible plastic
barrier allows the package to mold itself to the contours of the food being packaged.
*PHF/TCS = potentially hazardous food that requires temperature control for safety
Source: FDA 2009 Food Code, Section 3‐502.12.
c18.indd 388 1/14/2016 9:36:05 PM
CHAPTER 18 Culinology® Applications in Food Processing 389
(for example, salmonella and E. coli 0157:H7) and spoilage
microorganisms (bacteria, molds, yeasts).26 Meats prepared
for the NASA space program have been irradiation‐sterilized
for years; they can’t afford to have astronauts getting food
poisoning in outer space!
Intensive research programs were initiated during the late
1940s under the Atoms for Peace program of the Atomic Energy
Commission (AEC). Numerous international and domestic
studies designed to study the safety and efficacy of food irradia-
tion have been conducted for nearly 75 years, including studies
by the U.S. Army Labs in Natick, Massachusetts. None have
reported a link between consumption of irradiated foods and
human illness. In 1981, after a long international effort to inves-
tigate the safety and wholesomeness of irradiated foods, the
United Nations Joint Committee on Irradiated Foods decisively
stated:
The irradiation of any food commodity up to an average dose
of 10 kGy presents no toxicological hazard; hence toxicologi-
cal testing of foods so treated is no longer required.27
But in spite of the many advantages food irradiation offers,
the process remains controversial. In an effort to make it
sound less threatening, a more consumer‐friendly term has
been developed—cold pasteurization—not only to distin-
guish it from heat‐related pasteurization and sterilization
processes but also to reduce the fear factor induced by the
word irradiation.
The unit of measure for food radiation energy is termed a
gray (Gy), which equals the absorption of 1 joule of energy per
kilogram of product. Pasteurizing food treatment is less than
10 kiloGrays (kGy) per kilogram of food, with the following
exposure classifications: low exposure (<1 kGy), for control of
ripening, sprouting, and insects; medium exposure (1 to 10
kGy), for shelf‐life extension, to reduce microbial load with
improved quality characteristics compared to thermally proc-
essed. High exposure (10 to 50 kGy) is only used for commer-
cial sterilization.28 Higher sterilization levels of irradiation are
not commonly used on foods because of adverse quality con-
siderations (for example, development of off colors and rancid
flavors due to increased free radical formation leading to oxi-
dative rancidity). Instead, these levels are reserved for steriliz-
ing medical instruments and sickroom supplies.
An irradiation processing facility requires extensive shield-
ing and safety systems to assure containment of high‐energy
radiation. Typically, food is conveyed into a chamber con-
structed of thick‐walled concrete (5 to 6 feet) with remote
operational control. Bulk or prepackaged foods are continu-
ously conveyed near the source of irradiation (for example,
radioisotopes cobalt‐60 and cesium‐137, or machine‐produced
x‐ray or electron beams) for a controlled treatment time. The
relatively low doses of irradiation yield the intended benefits
without compromising product quality or package integrity.
Such processes are increasingly important in the control of
pathogenic microorganisms in meat, poultry, and seafood. It is
important to note that irradiation is less successful in eradicat-
ing botulinum spores or viruses than are traditional thermal
processes.
The FDA has approved a variety of foods for irradiation in
the United States, including:
Beef and pork
Poultry
Molluscan shellfish ( for example, oysters, clams, mussels,
and scallops)
Shell eggs
Fresh fruits and vegetables
Lettuce and spinach
Spices and seasonings
Seeds for sprouting (for example, alfalfa sprouts, respon-
sible for dozens of outbreaks of foodborne illness due to
pathogens residing in the seeds)
All irradiated foods must be labeled with a specially
designed symbol, commonly referred to as the radura.
Other Advanced Technologies
Research and development efforts continue to find innova-
tive and efficient new technologies to preserve food by alter-
native methods that yield minimally processed foods with
better eating qualities but the same level of safety as older,
more severe methods. The following are a few of the innova-
tive technologies currently being explored. Some have
achieved a fair degree of commercial success, while others
are still on the cusp of a major market breakthrough.
High‐Pressure Processing (HPP)
Also known as pascalization, HPP is a method of food process-
ing where food is subjected to elevated pressures (up to 87,000
pounds per square inch, or approximately 6000 atmospheres),
leading to the inactivation of most types of spoilage microor-
ganisms and pathogens. It is less successful in destroying
FIGURE 18.25 Radura symbol, required by the
FDA to appear on packaging of all foods treated
by irradiation.
Source: FDA, “Food Irradiation: What You Need to Know”
(June 2011).
c18.indd 389 1/14/2016 9:36:05 PM
390 CULINOLOGY®
bacterial spores. Pascalization stops chemical activity caused
by microorganisms that play a role in the deterioration of
foods. The treatment typically takes place at low tempera-
tures, but HPP may be combined with heat to achieve an
increased rate of inactivation of microbes and enzymes.
Process time is very rapid (usually less than 20 minutes of
exposure time) and does not require any food additives to be
effective.
In pascalization, food products are sealed and placed in a
steel compartment containing a liquid, usually water, and
pumps are used to create pressure. The treatment works
equally well for solid and liquid products, with or without
packaging. However, foods decrease in volume as a function
of the imposed pressure and equal expansion occurs on
decompression. For this reason the packaging used for HPP‐
treated foods must be able to accommodate up to a 15 percent
reduction in volume, and return to its original volume, with-
out loss of seal integrity and barrier properties.
In addition to microbial inactivation, during pascalization
the food’s proteins are denatured but most product’s main
structure remains intact. That said, high hydrostatic pressures
can cause changes in structurally fragile foods such as straw-
berries or lettuce. Pascalization does not greatly affect the
food’s nutritional value, taste, texture, or appearance if process-
ing takes place at room temperature or below. High‐pressure
treatment of foods is regarded as a natural preservation
method because it does not employ chemical preservatives.
An early use of pascalization in the United States was to
treat guacamole. It did not change the guacamole’s taste, tex-
ture, or color, and the refrigerated shelf life of the product
increased to 30 days from 3 days without the treatment.
Treated foods still require cold storage because pascalization
does not stop all enzyme activity, or some bacterial spores,
which can affect safety.
Examples of HPP products commercially available in the
United States (besides guacamole) include fruit smoothies,
prepackaged deli meats, fruit juices, and salsa.29 More recent
HPP foods include clams, oysters, and lobsters that have
been pressure‐shucked from their shells.
Ohmic Heating
Ohmic heating is an advanced thermal processing method
wherein the food material, which serves as an electrical resis-
tor, is heated by passing electricity through it. Electrical
energy is transformed into rapid and uniform heating that
quickly destroys food pathogens and spoilage microorgan-
isms. This inductive heating process (similar to induction
cooktops) uses electrical currents of oscillating electromag-
netic fields generated by electric coils in the vicinity of food.30
Ohmic heating can be used for heating liquid foods contain-
ing large particulates, such as soups, stews, fruit slices in syrups
and sauces, and heat‐sensitive liquids. The technology is useful
for the treatment of proteinaceous foods, which tend to dena-
ture and coagulate when thermally processed. For example,
liquid egg can be ohmically heated in a fraction of a second
without coagulating. Juices can be treated to inactivate
enzymes without affecting flavor. Other potential applications
of ohmic heating include blanching, thawing, in‐line detection
of starch gelatinization, peeling, dehydration, and juice extrac-
tion. The shelf life of ohmically processed foods is comparable
to that of canned and sterile or aseptically processed products.
At this point, ohmically treated market products in the
United States are limited, but as equipment costs come
down, many more products are expected to enter the market
in the next few years.
Radio‐Frequency (RF) Drying
Radio-frequency (RF) drying can offer many benefits over
conventional drying, including faster line speeds, more con-
sistent moisture levels, lower drying temperatures, and
smaller equipment. Conventional heating (for example, con-
duction, convection, radiant) has a heat source on the out-
side and relies on transferring the heat to the surface of the
material and then conducting the heat to the middle of the
material. RF heats at the molecular level, so it heats from
within the material and heats the center as well as the sur-
face. RF drying uses the same principles of heating by electro-
magnetic energy as do induction and microwave heating.
RF drying has many advantages over conventional meth-
ods, but it is still not used widely for commercial purposes
because of technical and economic reasons. Although numer-
ous research reports show the quality of RF‐dried products is
better than or equal to that of conventionally dried foods,
most of those studies are conducted on laboratory‐scale
devices, which may not be scalable for commercial opera-
tions. Major challenges include non‐uniform heating and
higher equipment costs compared to conventional drying
methods. To make RF drying practical and acceptable to
the industry, more significant scientific and technological
advancements are needed.
Pulsed‐Light Processing (PL)
Pulsed‐light (PL) technology is an alternative to thermal treat-
ment for killing pathogenic and spoilage microorganisms in
foods, including bacteria, yeasts, molds, and viruses. Materials
to be treated are exposed to multiple short flashes (typically
300 microsec) of a broad-spectrum (200 to 1100 nm), high‐
intensity white light to inactivate microorganisms.31 The exact
mechanisms by which pulsed light causes cell death are not
yet fully understood, but it is generally accepted that the anti-
microbial effects of UV light on bacteria are due to structural
changes in their DNA as well as other factors. Studies also
indicated similar injurious effects on yeast cells and mold
spores following exposure to pulsed light.
Pulsed-light treatment is able to inactivate pathogenic
and spoilage microorganisms in a range of food products and
packaging materials, but the treatment is most effective on
smooth, nonreflecting surfaces and in liquids that are free of
suspended particulates, such as clear fruit juices and water.
One of the potential applications of pulsed light is surface
decontamination of fresh fruits and vegetables and ready‐to‐
eat products such as deli meats. A recent study has reported
c18.indd 390 1/14/2016 9:36:05 PM
CHAPTER 18 Culinology® Applications in Food Processing 391
that peanuts exposed to pulsed-light treatment lose 90 per-
cent of their allergenicity.32 For any PL treatment to be fully
effective, uniform, complete exposure of the treated food is
critical. No significant changes of product quality or sensory
properties have been observed.
PL technology has been successfully tested as a technique
for pasteurization of food and packaging surfaces, but it is not
yet widely adopted mainly due to the high cost of equipment.
Commercial Meat
and Poultry Processing
Marination
The term marination, believed to have Latin‐French‐Spanish
origins, refers to the ancient practice of preserving foods in
seawater. Meats, fish, and poultry are the foods most
associated with marination. When fruits and vegetables are
similarly treated, the term used is maceration.
Marination was historically used as a preservation tech-
nique and to provide or enhance flavor. From a food proc-
essing and business perspective, increasing product yield is
today the single most important purpose of marination.
There are two typical yield perspectives: the percentage of
marinade pickup (pump) and the cooked yield. Pickup per-
centage is determined by: [(marinated weight – raw weight)
/raw weight] × 100. Cooked yield is (cooked weight/raw
weight) × 100.
Commercial marinade ingredients enhance tenderness,
reduce cooking losses, and increase the water‐holding capac-
ity and thus the yield of marinated food.33 From a culinary
perspective, marination is primarily organoleptic in that it
adds flavor and moisture and enhances appearance; second-
arily, it tenderizes and softens.34 In this respect, yield enhance-
ment is not the primary objective.
Marination at the home or restaurant level usually involves
the passive transport of marinade into foods by absorption or
osmosis and is a slow process known as static marination.
Unless the food in static marination is very thin, the marinade
does not travel far into it. Even after marination of 24 hours or
more, marinade pickup seldom exceeds 5 percent. Commercial
meat processors obviously require a faster and more deeply
penetrating result.
Modern marination techniques, functional ingredients,
equipment, and technology continue to be refined and
updated to improve efficiency, quality, and consumer satis-
faction. Commercial marination is performed using func-
tional marinades applied through highly automated systems.
To decrease process times and increase uniformity of mari-
nade distribution, specialized equipment is used to inject the
marinade deep into the muscle. This process, referred to as
deep basting or injection marination, allows for marinade
uptake to exceed 20 percent or more within an extremely
short period. It is often followed by vacuum tumbling to
ensure uniform distribution of the marinade throughout the
muscle, to avoid localized discoloration and the development
of hot pockets of marinade centered on the injection needle
tracks, and to enhance tenderness via mechanical action as
the meat moves around inside the tumbler.
Tumble‐marinating continually mixes the meat and mari-
nade ingredients in a large rotating drum, usually under vac-
uum. This assists in maximum uptake and uniform internal
dispersion of the marinade, even without injection, while
tenderizing the food product. The marinade ingredients may
help tenderize the food in question; however, tenderization is
enhanced as the muscle fibers are massaged during tum-
bling. The tumbling leads to disruption of the muscle fiber
sheaths and stretches the myofibrils.35 Vacuum tumbling can
achieve in 30 minutes or less what otherwise would take sev-
eral days using static marination, depending on the size and
type of muscle being marinated.
Salt (sodium chloride, or NaCl) is a critical and primary
marinade ingredient in that it helps increase the meat’s mois-
ture‐holding (marinade‐holding) capacity and thus increases
product yield and palatability. Salt rapidly hydrates and solu-
bilizes the salt‐soluble proteins predominant in meat tissue,
thus dramatically enhancing the water‐holding capacity and
the stickiness of the meat. The salt‐soluble proteins combine
with water through both direct molecular interactions
(adsorption) and physical entrainment (absorption); again,
this increases meat weight, volume, and flexibility. The sticky
marinated meat forms a binding glue‐like layer of extracted
proteins on its surface; this denatures or coagulates on heat-
ing and forms a rigid firm mass. This is the principle used in
preparation of many formed meat products, such as chicken
nuggets and meat loaves.
Many other ingredients may be used in commercial mari-
nades. Proteolytic enzymes tenderize the meat by hydrolyz-
ing the muscle proteins—that is, the proteins are broken
down to their component amino acids. Flavor enhancement
may also occur, as glutamic acid (an amino acid and the key
flavor enhancer in monosodium glutamate [MSG]) is also
released. The most commonly used tenderizing enzymes are
papain and bromelain, derived from papaya and pineapple,
respectively. However, ficin (from figs), fungal enzymes (from
fungi such as Aspergillus oryzae), bacterial enzymes (from
bacteria such as Bacillus subtilis), and combinations of these
are also used.
Alkaline phosphates increase protein solubility and enhance
the water‐binding ability of meats, leading to increased
tenderness and juiciness of processed meats. In general, alkaline
phosphates improve water retention by shifting the pH away
from the isoelectric point of the myofibrillar proteins (the point
at which the water‐holding capacity of meat is at its lowest) and
by unfolding muscle proteins, thereby exposing more charged
sites for water binding. When phosphates are used for increas-
ing water‐holding properties of meat, the USDA requires
phosphate concentrations to be no higher than 0.5 percent of
the finished product weight. This is just as well, as excess
phosphate can cause soapy flavors, rubbery texture, and poor
color. Although there are many phosphates to choose from,
sodium tripolyphosphate (STP) remains the most commonly
utilized in marinades because it is easy to use and inexpensive.
c18.indd 391 1/14/2016 9:36:05 PM
392 CULINOLOGY®
STP accounts for approximately 80 percent of the phosphates
used in further‐processed meat products. Alkaline phosphates
such as STP serve to increase water‐holding capacity (WHC),
extract muscle proteins, increase cook yield, reduce oxidative
rancidity, preserve meat color, increase flavor retention, and
inhibit microbial growth.
Product yield and palatability are also increased through
the use of starches or by adding soy or whey proteins.36
Hydrocolloids (vegetable gums from terrestrial or marine
sources) are also commonly used for additional water‐holding
capacity and subsequent yield enhancement. Flavorings, herb
and spice extracts, antioxidants, liquid smoke, sweeteners—
virtually any water‐soluble or dispersible ingredient can be
added to a marinade, depending on the desired outcome.
Marinade Types
Oily Marinades
Dry or lean meats benefit from oil‐based marinades because
they add fats that increase the palatability and the organ-
oleptic (sensory) qualities of the food. The oil makes the food
appear moister, protects it from drying during cooking,
improves mouthfeel and lubricity, provides an attractive
shine, inhibits oxidation, and assists in the flavor profile as it
provides fat‐soluble flavors.37
The flavors in oil may be inherent or augmented by way
of infusion or the addition of oil‐soluble flavor extracts. Oils
should be considered from the perspective of their
saturation profile and their individual fatty‐acid profile,
which have nutritional, shelf life, and flavor implications.38
Acidic Marinades
Many marinades contain an acidic ingredient. Historically, it
was vinegar or verjus, the juice of unripe and therefore acidic
grapes. Other acidic ingredients include wine, fruit juices,
and cultured milk products such as buttermilk, yogurt, kefir,
and sour cream. The amount of acid in a marinade varies
greatly. The highest acid levels are used in pickling, or directly
acidifying, fruits or vegetables with a typical acetic acid level
of 2.5 percent.39 Cultured milk products are mildly acidic,
with the lactic acid ranging from 0.5 percent to 1 percent, and
even less when diluted for use in a marinade.
The acidic element in marinades plays several roles. Of
course it adds flavor; in addition, it disrupts the microbial
cells it touches. Weak acids, such as the acetic and lactic
acids often used in marinades, are lipophilic and pass through
the lipid‐containing membranes of the microbial cells. Once
FIGURE 18.26 Marinade ingredient functionality.
Salt (NaCl) Flavor enhancer
Moisture binder
Increases cooked yield
Increases palatability/juiciness
Helps extract soluble proteins
Increases water absorption
Binds restructured protein‐based
products
Phosphates Moisture binder
Increases cooked yield
Increases palatability/juiciness
Reduces purge
Helps extract soluble proteins
Increased water absorption
Binds restructured protein‐based
products
Antioxidant
Reduces oxidative rancidity
Flavor stabilizer
Color stabilizer
Fat emulsifier
Enhances mouthfeel
Texture stabilizer
Softens muscle tissue
Starches
and gums
(hydrocolloids)
Moisture binder
Increases cooked yield
Increases palatability/juiciness
Enhances mouthfeel
Texture stabilizer
Increases storage stability
Freeze‐thaw
Emulsifier
Provides viscosity and film formation
Assists in marinade dispersion
Helps retain liquids
Flavorings Provides desired taste profile
Improves basic flavors
Restores flavors lost during processing
Provides proper ethnic profile
Inhibits or masks “warmed‐over” or
oxidized flavors
Masks undesirable flavors from additives
Water Increases juiciness
Enhanced yield
Marinade ingredient delivery vehicle
Oil Increases mouthfeel
Adds lubricity/tenderness
Perceived juiciness
Flavor carrier
Sugars Enhances flavor
Enhances browning
Increases shelf life
Source: Mark A. Uebersax, Ph.D., Muhammad Siddiq, Ph.D.,
Carl P. Borchgrevink, Ph.D.
c18.indd 392 1/14/2016 9:36:05 PM
CHAPTER 18 Culinology® Applications in Food Processing 393
inside the cytoplasm of the microbial cell, the acid molecules
release protons and can no longer pass back out through the
cell membrane, thus acidifying the cytoplasm. This inhibits
cellular metabolism and growth, which can slow or fully
inhibit deterioration and spoilage, depending on the microbe.
Acidic ingredients used in marinades may have a relatively
neutral flavor profile (apart from their acidity), as in distilled
white vinegar, or a strong flavor profile, as in fortified wine
(such as fino sherry) or in citric juice (such as that pressed
from tangerines). Acidic liquids with neutral flavor profiles
can be infused with herbs, spices, and fruit and vegetable
peels, or they can be flavored with extracts or chemically
derived flavors. The choice and amount of acidic ingredients
depends on the desired level of acidity, the desired flavor pro-
file, and their effect on the marinated product.
Acidic marinades are also widely used in the preparation
of the popular Latin American seafood specialty known as
“ceviche.” These are highly seasoned, usually citrus juice–
based marinades that are used to flavor and “parcook” the
seafood (partially denature the proteins) without actually
applying any heat. The resulting products are essentially raw,
resembling Japanese sashimi (“raw fish”) in texture, but unlike
sashimi, ceviche is strongly flavored.
Salty Marinades
Most resources use the term brine when they refer to salty
marinades.40 Brines are often applied to lean meats, as the
salt helps counter the water loss that takes place during
cooking. Salt‐denatured myofibrillar protein filaments have
increased water‐holding capacity and absorb more of the
marinade. As stated previously, the marinated meat may
absorb 10 percent or more of its weight from the marinade if
marination is combined with vacuum tumbling. The salt‐
denatured protein does not coagulate as readily when
cooked, staying softer and palatable when eaten, providing a
sense of increased tenderness.41
Cured meats, such as ham, bacon, and corned beef, are
treated with brines that usually contain nitrites, which set
the pink color typical in these meats and inhibit the growth of
Clostridium botulinum.
Fruits and vegetables can also be treated with brines for
pickling; alternatively, they may be treated with dry salt and
allowed to develop their own marinade by drawing liquids
out from within, as is typical with sauerkraut or its Asian
cousin, kimchi. Whether a fruit or vegetable is dry‐salted or
brined, the intent is to draw liquid out of the produce so it
becomes submerged, minimizing oxygen exposure. More
importantly, the goal is to produce an environment condu-
cive to lactic acid bacteria, leading to fermentation, which is
one of the oldest methods of preservation known to man.
Dry Rubs and Pastes
In essence, a rub is a dry marinade. It may contain salt, sugar,
spices, and herbs in a variety of combinations. If the mixture
contains some moisture, it is called a paste rather than a rub.
FIGURE 18.27 Commercial marinade formula
for chicken.
Ingredients %
Chicken 85
Marinade 15
Total 100
Marinade Components %
Water 10.0–13.0
Flavor 1.0–3.0
Starch 0.5–1.0
Salt 0.5–1.0
Phosphate 0.3–0.5
Total 15.0
Basic Marinade Preparation
1. Dissolve phosphate in water.
2. In a separate container, blend dry flavors, starch, salt,
and other dry ingredients. If oils, flavor oils, or oleoresins
are used, plate on dry blend.
3. Add liquid flavors to water/phosphate solution with
high‐shear mixing.
4. Add dry ingredients from Step 2 to water/phosphate
solution with high‐shear mixing.
5. Mix well for 5 minutes.
6. Proceed with marination.
Phosphate Note!
Always dissolve phosphate in water first as it
competes for binding sites on the water molecule with
other ingredients. If phosphate is added later, it will
not go into solution. Other dries may be pre‐blended
together for ease of addition after the phosphate is
completely dissolved. Mix well for 5 minutes after all
ingredients have been added. An immersion blender
(“stick” blender) is a good tool to use for this process.
Potential Problems:
Plugged injectors: Caused by particulates, ground
spices, hard fats, viscosity build up.
Hot spots: Uneven distribution of marinade due to lack
of tumbling after injection or improper needle
placement.
Bacterial growth: Caused by inadequate temperature
control.
Low pick‐up: Caused by inadequate quantity of mari-
nade, insufficient marination time, plugged or improp-
erly placed nozzles, inadequately thawed product,
insufficient vacuum, inadequate quantity of chicken in
the tumbler.
Source: J. Cousminer.
c18.indd 393 1/14/2016 9:36:05 PM
394 CULINOLOGY®
The moisture may be from freshly crushed aromatics (such as
onions or garlic), from oils, juices, or wine, or simply from
chopped fresh herbs.42 Rubs and pastes are good when excess
moisture is not desired, as when a flavorful crust is needed;
items using rubs or pastes may require gentler heat when
cooking to avoid burning.43
While rubs and pastes are used primarily to provide flavor
and enhanced appearance, the spices and herbs may also act as
antimicrobial agents,44 and salt and sugar both draw moisture
from living bacterial cells through osmosis, disrupting cellular
activity and thus microbial growth.45 However, as rubs and
pastes do not penetrate much beyond the surface of the mus-
cle, flavors are mainly limited to the meat’s surface, and they do
not increase product yields or improve texture or lubricity.
Conclusion
Food processing can significantly enhance the texture, color,
flavor, aroma, nutritional availability, palatability, safety, shelf
life, convenience, cost and consistency of raw products. The
edibility of numerous staple commodities such as dry beans,
potatoes, rice, and pasta is improved due to the hydration,
maceration, and cellular separation (cell membrane and cell
wall disintegration) that we recognize as cooked food and
that occurs during moist‐heat processing. Heat treatments,
including blanching and pasteurization, inactivate many
native enzymes that can impair sensory attributes (for exam-
ple, oxidation of color, development of off flavors, rancidity)
and can improve texture by hydrolysis of proteins, carbohy-
drates, and fiber. Additionally, processing foods offers specific
functional properties (for example, gelatinization of starch in
aseptically processed puddings, coagulation of proteins in
cheese and emulsified meats, gelation of pectins in fruit pre-
serves). And, of course, an assortment of processing methods
is used to improve the safety and shelf life of raw foods by
controlling pathogens and spoilage organisms.
The Culinology® professional clearly recognizes and appre-
ciates the numerous benefits provided by food processing,
welcomes the high quality, convenience, and affordability of
using processed foods as ingredients in prepared dishes, and
makes good use of them every day.
1R. S. Harris and E. Karmas, Nutritional Evaluation of Food Processing
(Westport, CT: AVI Publishing, 1975); A. E. Bender, Food Processing and
Nutrition (London: Academic Press, 1987).
2E. Laurila and R. Ahvenainen. “Minimal Processing in Practice,” in T. Ohlsson
and N. Bengtsson (eds.), Minimal Processing Technologies in the Food
Industry (Cambridge, UK: Woodhead, 2002), 223.
3“Fresh Cuts Rebounding,” in Fresh Cut Magazine ( January 2011), retrieved
February 17, 2015, from http://freshcut.com/index.php/magazine/issue
/january‐2011.
4A. M. Piagentini, D. R. Guemes, and M. E. Pirovani, “Sensory Characteristics
of Fresh‐Cut Spinach Preserved by Combined Factors Methodology,” Journal
of Food Science 67, no. 4 (2002): 1544–1549.
5M. Siddiq, “Peaches and Nectarines,” in Y. H. Hui (ed.), Handbook of Fruits
and Fruit Processing (Ames, IA: Blackwell, 2005), 519–531.
6L. Vamos‐Vigyazo, “Polyphenol Oxidase and Peroxidase in Fruits and Vege-
tables,” Critical Reviews in Food Science and Nutrition 15, no. 1 (1981): 49–127.
7H. S. Lambrecht, “Sulfite Substitutes for the Prevention of Enzymatic
Browning in Foods,” in C. Y. Lee and J. R. Whitaker (eds.), Enzymatic
Browning and Its Prevention (Washington, DC: American Chemical Society,
1995), 313–323.
8R. Soliva‐Fortuny, P. Elez‐Martinez, and O. Martin‐Belloso, “Microbiological
and Biochemical Stability of Fresh‐Cut Apples Preserved by Modified
Atmosphere Packaging,” Innovative Food Science and Emerging Technologies
5, no. 2 (2004): 215–224.
9James Steffe, personal communication, 2009.
10N. Appert, Le Livre de Tours les Ménages, ou L’Art de Conserver, Pendant Plusieurs
Années Toutes les Substances Animales et Vegetales (Paris: Chez Patris,
1810).
11J. L. Heid and M. A. Joslyn, Fundamentals of Food Processing Operations:
Ingredients, Methods, Packaging (Westport, CT: AVI Publishing, 1967).
12J. M. Jackson and B. M. Shinn, Fundamentals of Food Canning Technology
(Westport, CT: AVI Publishing, 1979).
13Heid and Joslyn, Fundamentals of Food Processing Operations.
14J. R. D. David, R. H. Graves, and V. R. Carlson, Aseptic Processing and
Packaging of Food: A Food Industry Perspective (Boca Raton, FL: CRC Press,
1996).
15D. K. Tressler, W. B. Van Arsdale, and M. J. Copley, Factors Affecting Quality in
Frozen Foods, vol. 2, The Freezing Preservation of Foods, 4th ed. (Westport,
CT: AVI Publishing, 1968).
16N. W. Desrosier and D. K. Tressler, Fundamentals of Food Freezing (Westport,
CT: AVI Publishing, 1977).
17W. B. Van Arsdale and M. J. Copley, Principles, vol. 1, Food Dehydration
(Westport, CT: AVI Publishing, 1963).
18P. Fellows, Food Processing Technology: Principles and Practice (Chichester,
UK: Ellis Horwood, 1988).
19J. M. Harper, Extrusion of Food (Boca Raton, FL: CRC Press, 1981).
20S. Bhatnagar and M. A. Hanna, “Extrusion Processing Conditions for
Amylose‐Lipid Complexing,” Cereal Chemistry 71, no. 6 (1994): 587–593.
21P. G. Creed and W. Reeve, “Principles and Application of Sous‐Vide Processed
Foods,” in S. Ghazala (ed.), Sous‐Vide and Cook‐Chill Processing for the Food
Industry (Gaithersburg, MD: Aspen, 1988), 25–56.
22V. K. Juneja and O. P. Snyder, “Sous‐Vide and Cook‐Chill Processing of
Foods: Concept Development and Microbiological Safety,” in G. Tewari and
V. K. Juneja (eds.), Advances in Thermal and Non‐Thermal Food Preservation
(Ames, IA: Blackwell, 2007), 145–163.
23Ibid.
24S. Ghazala and R . Trenholm, “Hurdle and HACCP Concepts in
Sous‐Vide and Cook‐Chill Products,” in S. Ghazala (ed.), Sous‐Vide and
Cook‐Chill Processing for the Food Industry (Gaithersburg, MD: Aspen,
1998), 294–310; Juneja and Snyder, “Sous‐Vide and Cook‐Chill
Processing of Foods.”
25For a detailed discussion, see Ghazala and Trenholm, “Hurdle and HACCP
Concepts in Sous‐Vide and Cook‐Chill Products”; Juneja and Snyder,
“Sous‐Vide and Cook‐Chill Processing of Foods”; J. M. Farber and K. L.
Dodds, Principles of Modified‐Atmosphere and Sous‐Vide Product Packaging
(Lancaster, PA: Technomic, 1995); Food and Drug Administration, FDA
Food Code 2009: Annex 6—Food Processing Criteria, retrieved November 30,
2009, from http://www.fda.gov/Food/FoodSafety/RetailFoodProtection
/FoodCode/FoodCode2009/ucm188201.htm#parta6‐2.
26D. G. Olson, “Irradiation of Foods: Scientific Status Summary,” Food Tech-
nology 52, no. 1 (1998): 56–62.
27United Nations Joint Committee on Irradiated Foods, Food Irradiation: A
Technique for Preserving and Improving the Safety of Food (Geneva,
Switzerland: World Health Organization & Food and Agriculture
Organization of the United Nations, 1988), 31. http://apps.who.int/iris/
bitstream/10665/38544/1/9241542403_eng .
28Council for Agricultural Science and Technology, “Ionizing Energy in Food
Processing and Pest Control: II. Applications,” Task Force Report No. 115
(Ames, IA: Council for Agricultural Science and Technology, 1989), 98.
c18.indd 394 1/14/2016 9:36:05 PM
http://freshcut.com/index.php/magazine/issue/january-2011
http://www.fda.gov/Food/FoodSafety/RetailFoodProtection/FoodCode/FoodCode2009/ucm188201.htm#parta6-2
http://apps.who.int/iris/bitstream/10665/38544/1/9241542403_eng
CHAPTER 18 Culinology® Applications in Food Processing 395
29R. Ramaswamy, V. M. Balasubramaniam, and G. Kaletunç, High‐Pressure
Processing: Fact Sheet for Food Processors (Columbus, OH: Ohio State
University, 2004), retrieved December 12, 2009, from http://ohioline.osu
.edu/fse‐fact/pdf/0001 .
30S. K. Sastry, Ohmic and Inductive Heating. Paper presented at the annual
meeting of the Institute of Food Technologists, Dallas, TX, 2000.
31H. Shaw, Report on Pulsed Light Processing of Seafood, report by Campden
and Chorleywood Food Research Association Group (CCFRA), Chipping
Campden, UK, 2008, retrieved January 13, 2010, from www.seafish.org/pdf
.pl?file=seafish/Documents/B14_Pulsed percent20Light .
32Rory Harrington, “Pulsed UV Light Cuts Peanut Allergens by Up to 90 Per
Cent: Study,” Food Production Daily, June 17, 2011, retrieved February 12,
2015 from http://www.foodproductiondaily.com/Processing/Pulsed‐UV
‐light‐cuts‐peanut‐allergens‐by‐up‐to‐90‐per‐cent‐study.
33R. M. Burke and F. J. Monahan, “The Tenderization of Shin Beef Using a
Citrus Marinade,” Meat Science 63, no. 2 (2003): 161–168; T. A. Williams,
Effects of Beef Enhancement with Non‐Meat Ingredients, Blade Tenderization,
and Vacuum Tumbling on Quality Attributes of Four Beef Cuts Stored in a
High‐Oxygen Environment, master’s thesis, Texas A&M University, 2004;
A. Önenç, M. Serdaroğlu, and K. Andraimov, “Effects of Various Additives
to Marinating Baths on Some Properties of Cattle Meat,” European Food
Research and Technology 218, no. 2 (2003): 114–117.
34J. Tarantino, Marinades: Dry Rubs, Pastes, and Marinades for Poultry, Meat,
Seafood, Cheese, and Vegetables (Freedom, CA: Crossing Press, 1992).
35L. A. Brandt, “Marinade Meat Challenges,” Prepared Foods, December 9, 2003.
36D. Berry, “Soaks and Sauces,” Food Product Design, January 20, 2010, retrieved
February 4, 2010, from http://www.foodproductdesign.com/ articles/2010
/01/soaks‐and‐sauces.aspx.
37H. McGee, On Food and Cooking: The Science and Lore of the Kitchen (New
York: Scribner, 2004); Tom Stobart, The Cooks Encyclopedia: Ingredients and
Processes (New York: Harper and Row, 1980); Tarantino, Marinades.
38A. Davidson, The Oxford Companion to Food (New York, Oxford University
Press, 1999); D. B. Min and T. H. Smouse (eds.), Flavor Chemistry of Lipid
Foods (Urbana, IL: American Oil Chemists Society, 1989); V. Dubois et al.,
“Fatty Acid Profiles of 80 Vegetable Oils with Regard to Their Nutritional
Potential,” European Journal of Lipid Science and Technology 109, no. 7
(2007): 710–732.
39McGee, On Food and Cooking.
40S. Labensky and A. Hause, On Cooking: A Textbook of Culinary Fundamentals
(Upper Saddle River, NJ: Prentice Hall, 2007); McGee, On Food and Cooking.
41McGee, On Food and Cooking.
42Tarantino, Marinades.
43M. Miller and M. Kiffin, Coyote’s Pantry (Berkeley, CA: Ten Speed Press,
1993).
44J. Billing and P. W. Sherman, “Antimicrobial Functions of Spices: Why Some
Like It Hot,” Quarterly Review of Biology 73, no. 1 (1998): 3–49.
45McGee, On Food and Cooking.
c18.indd 395 1/14/2016 9:36:06 PM
http://ohioline.osu.edu/fse-fact/pdf/0001
http://www.foodproductiondaily.com/Processing/Pulsed-UV-light-cuts-peanut-allergens-by-up-to-90-per-cent-study
http://www.foodproductdesign.com/ articles/2010/01/soaks-and-sauces.aspx
http://www.seafish.org/pdf
396 • CULINOLOGY®
19
Commercializing
the Culinary Gold
Standard
Introduction
Product development is a formal and disciplined yet highly
creative process that begins with an idea and ends with the
launch of a new (or improved) product. Easier said than done .
Creating a culinary gold standard as the result of an idea,
and successfully commercializing it, requires the combined
knowledge of culinary art, food science, manufacturing
process and packaging technology, engineering, customer
and consumer research, food safety and sanitation, quality
assurance, marketing, demographic and trend awareness,
finance, government and regulatory requirements, ingredient
procurement, storage and distribution, and—above all—strong
proj ect management skills. The Culinology® professional ’ s
ability to work and communicate within a cross‐functional
team of specialists in each of these areas is critically impor-
tant. Successful commercialization is where the rubber
meets the road for the Culinology® professional.
Commercialization is the process of converting a culinary
gold standard into a manufacturable form and introducing a
new product into the market. A gold standard is a food or
beverage product, developed on sound culinary principles,
that is used as a benchmark throughout the commercializa-
tion process. In a perfect world, the commercialized prod-
uct closely replicates the product attributes of the gold
standard.
The actual launch of a new product is the final stage of
new product development and where the most money is
likely be spent (for advertising, sales promotion, and market-
ing efforts). In general, it costs a dollar of investment for every
dollar of sales achieved in the first year. Early in the process,
costs may be relatively low, but as the project moves forward,
investments in customer or consumer research, engineering
and equipment, production trials, and launch expenses con-
tinue to increase; it ’ s far better to kill a poor idea early in the
process than for it to fail in the marketplace before it even
begins to pay for its development costs.
Commercialization of a product (moving from idea through
launch, or from mind to market) is successful only if the fol-
lowing four questions are answered.
When to Launch?
The company must decide on the timing of the introduc-
tion. Many factors influence optimal launch timing; a few are
listed here:
• The danger of cannibalizing the sales of current well‐
performing products
• Menu or store‐shelf reset dates (those times when an
existing display or shelf of products is re‐arranged) for
key customers
• Shortage or seasonality of production capacity, labor, raw
materials, or marketing dollars to support a successful
launch
• The need to be first in market even if the product is not
yet optimized—finding the balance between the perfect
product and the perfect launch timing in a highly com-
petitive market
• Economy or trend indicators—if they are down, it may
not be the right time to launch
The best, most innovative products can still fail if launched
prematurely (or too late!).
Where to Launch?
The company must decide where to launch. It may be in a
single location, one or several regional launches, or a national
or even international launch. This decision is strongly influ-
enced by the company ’ s capital, managerial confidence, and
Lead Author: Marilyn Carlson, CFS , Owner/Principal , Dogwood Solutions
Curator of Culture: Chef Danhi & Co/Global Flavor Shakers
CRC®, CCS®, CEC, CCP, Manager of Culinary Solutions, Sensient Flavors
Robert Danhi,
Craig “Skip” Julius ,
c19.indd 396 1/22/2016 1:22:09 PM
CHAPTER 19 • Commercializing the Culinary Gold Standard • 397
operational capacities. Smaller companies usually opt for
smaller local or regional launches, while larger companies,
with more financial muscle and national brand recognition,
may choose to enter the entire market at the same time.
Global rollouts are generally undertaken by multinational
conglomerates with the requisite size and global facilities
and resources to support multiple countries at once. (Similar
to regional rollouts, the multinational strategy may also use a
“by‐country” approach, introducing the new product in one
country or region at a time.)
Of course, with the ubiquity of television advertising and
Internet sales, even the smallest companies now have imme-
diate access to the entire marketplace wherever there is a TV
set, a computer, or a smartphone.
Who is going to buy
my product?
The company must identify target consumers exactly: their age,
gender, marital status, ethnic identity, income level, social atti-
tudes, category purchase activity (or buying preferences), geog-
raphy, and so on. Primary target consumer groups are generally
identified for a specific brand or product positioning, and new
products developed for this brand or positioning have the appro-
priate target market in mind from the beginning of develop-
ment. In this way, limited and costly distribution and promotion
resources may be focused to improve the chances of a strong
positive response during the initial launch or rollout phase.
How am I going to do it
(successfully)?
Simply put, the company must develop a viable marketing
budget and determine an action plan for introducing the
product into the marketplace. Successfully.
Product Development:
The Benefits of Using
a Proven Process
Product launches are notoriously unsuccessful. Most food
products fail because they simply do not meet customer or
consumer expectations for value, performance (flavor, tex-
ture, eating quality), and/or convenience. A proven key to
commercial success is generating better consumer research
and information at the front end of the development process
to ensure the right end‐user needs are identified and met. No
amount of execution makes up for an inherently weak idea;
conversely, few great ideas survive weak executions.
The Product Development Management Association
(PDMA) reports that a large percentage of consumer pack-
aged goods companies use a stepped idea‐to‐launch approach
to product development, such as the Stage‐Gate® process.
This sort of system is essentially an operational roadmap for
moving a new product from idea to launch. The process logi-
cally breaks the project into distinct stages with decision‐point
“gates” separating each stage. Cross‐functional product devel-
opment teams must complete a prescribed set of related activi-
ties in each stage prior to obtaining management approval to
proceed through the gate to the next stage of development.
Activities in any given stage are designed to gather vital techni-
cal, market, financial, and operational information, with the
focus on minimizing risk and maximizing speed to market.
Each stage along the development path has a potential cost
greater than that of the one preceding it; with a stepped
process, expenditures are allowed to rise as uncertainties
decrease and risk is managed.
The benefits of the idea‐to‐launch process are based on
the attention to detail used in developing and setting up the
process. Each stage must be crafted to bring the right infor-
mation to the key decision makers (“Gate‐Keepers”) to gain
approval to move forward. Such information includes: defin-
ing the business opportunity, understanding end‐user needs
and the key product attributes that will meet them, technical
and manufacturing feasibility, product and process develop-
ment and manufacturing costs, including capital expendi-
tures, and many other considerations. A well‐defined process
includes the following attributes, often customized to the
company, business unit, or even brand group:
• Based on reality—the need or opportunity is clearly
defined.
• Establishes measurable benchmarks.
• Defines the gold standard target, using consumer and
customer input.
• Introduces discipline into what can be a chaotic process.
• Manages allocation of scarce or expensive resources.
• Ensures a complete process—no critical steps are
omitted.
• Eliminates politics and emotion from decision making.
• Accelerates speed to market via a well‐defined process
that also reduces wasted resources (people, time, dollars).
• Increases likelihood of product success—weak ideas are
weeded out early in the process due to increased focus
and discipline.
A well‐defined idea‐to‐launch process is not limited to
large companies; smaller companies are likely to have shorter,
less complex processes by virtue of their less complex organi-
zational structure. Any company disciplined enough to
develop a solid idea‐to‐launch process that meets its organi-
zational needs and addresses company culture can benefit.
The Product
Development Process
Identifying a Need
Key questions that must be answered include these, and
often many others:
• What is it?
• Who wants it?
c19.indd 397 1/14/2016 7:56:32 AM
398 • CULINOLOGY®
• What problem does it solve?
• How does it work?
• What is its competitive point of difference?
• How will it be profitable?
• How will it be manufactured?
An organization’s ability to detect, target, and capture the
best new product opportunities is a critical success factor in
this dynamic and competitive industry.
The need for a new product may be driven by internal
forces such as brand innovation or renovation, identified
portfolio gaps, profit improvement, sales force insights, and
market research; or by external forces such as emerging
trends and changing consumer patterns, competitive pres-
sure, economic forces, advances in technology, and a chang-
ing marketplace (examples include obesity and health concerns,
social media, internet marketing and sales, and eco‐friendly
or sustainability initiatives).
Once the needs are identified, it’s time to build the
concept(s) based on them. Key concept components include
a clear objective, the marketing rationale (problems and
solutions), product and process characteristics, initial fea-
sibility analyses (such as equipment and resource needs,
launch timing, shelf‐life requirements, and food quality
and safety issues), manufacturing and distribution consid-
erations, and financial and cost targets, including cost of
goods sold (COGS).
The result of this initial work is an ability to:
• Define the need or opportunity and defend it.
• Describe the value proposition—an analysis and quanti-
fied review of the benefits, costs, and value that an organi-
zation can deliver to customers. It is also a positioning of
value where value = benefit – costs (includes risk).
• Build the business case and develop the project scope.
Scope definition is a core project‐planning process;
it involves identifying and describing the work required
to produce the desired outcome (in this case, a par-
ticular new product or perhaps product line). Good scop-
ing includes defining both what the project is and is not
intended to deliver. Clearly identifying what is not within
the scope helps avoid “scope creep” as the project evolves.
• Provide a project brief, including constraints, that
includes all of the above.
Defining the Vision
The next phase is to generate ideas that address the defined
need. Think of idea generation as a funnel; it is essential to
look at many ideas to come up with one or two products (or
product lines) that can be sustained long‐term, especially in
this age of constant change. It is vital to involve key internal
and external stakeholders, including customers, early in the
process. The key output of the process is a clearly articulated
basic product description and its target consumer(s).
FIGURE 19.1 Example of an idea-to-launch process.
Initial Phases—Idea and Product Development
Step 1:
Consumer and
Operator
Research
Focus Groups • Customer and
Consumer
Problems and
Needs
• Screened and
Prioritized Top
Ideas for Further
Exploration
IDEAS
CONCEPT X
Details:
• Market-shaped
Concepts
• Market-
shaped and
Tested
Prototypes
• Positioning and
Roll-out Plan
• Business Cases
and Portfolio
Development
Plan
• Categorized
Pipeline of Ideas
• Opportunity
Areas
Sales Force
Competitive
Review
Strategic and
Financial
Criteria
Step 2:
Identify
Opportunity
Areas
Step 3:
Generate and
Screen
Ideas
Step 4:
Develop,
Shape,
and Analyze
Concepts
Step 5:
Develop and
Test Prototypes
Source: Craig Julius.
c19.indd 398 1/14/2016 7:56:33 AM
CHAPTER 19 • Commercializing the Culinary Gold Standard • 399
FIGURE 19.2 Example of a product opportunity document.
Opportunity Name: Date:
Product Category: Category Manager:
Summary / Rationale of Opportunity [i.e., Financial, Strategic, etc.]:
Description of Product(s):
Reason for Opportunity [check all that apply]:
Brand Information:
Existing Competitors:
Manufacturer / Brand Name: _____________________
Description: ______________________
Existing Price Points: ______________________
Our Point of Differentiation: __________________
Segment Target [check all that apply]:
Foodservice__ Healthcare__ K–12__ C&U__ Chains ___ Club__ C‐Store__ Military___ Retail___
Estimated Yearly Sales $:
Target Cost [Price/Unit]:
Target Sell [Price/Unit]:
Estimated Contribution Margin $ Estimated Margin %:
Product Attributes [check all that apply]: Vegan __ Vegetarian __ Kosher __ Halal __ Low Sodium __
Other _______________________________
Organic __ Sugar Free __ Low Fat __ Trans Fat Free __ Child Nutrition __ Fair Trade __ Green __ Natural __
Suggested Operator’s Cook / Re‐Thermalize Process [check all that apply]: Fry ___ Steam ___
Microwave ___ Bake ___ Broil ___ Grill ___ Stove Top ___ Boil ___
Packaging / Storage Consideration
Preferred Packaging Material: Inner: ______________________________Outer: ________________________
Est. Case Weight: _________ or Est. Case Count: ________
Units per Master Case: _____
Other Considerations [check all that apply]: Tamper Evident ___ Reseal capable ___ Recyclable ____
Biodegradable ___ Ovenable____
Storage Type: Freezer ____ Cooler ____ Dry Goods ____
Desired Shelf Life [Days]: ___________
Launch Date Estimate: Quarter _____ Year ____
POP Approval: Yes: _____ No: _____ If no, why? ___________________________________________
Signatures Required:
___________________ Date: ______
___________________ Date: ______
___________________ Date: ______
Source: Craig Julius.
A key component of concept development are ideation
sessions, which can be conducted internally if the company
has a proven session facilitator on staff. However, many
highly experienced external resources are available to help
with this; the money will be well spent. Interdisciplinary ses-
sion participants bring more to the table and provide a feast
of ideas; it’s important to include representatives of major
internal departments such as culinary, food science, sales
and marketing, procurement, manufacturing, and regulatory,
as well as external participants—regardless of an employee’s
professed objectivity, they still work within the company and
place an unconscious filter on what they hear or say.
Good ideation often includes a competitive product review
(often called market benchmarking). Identifying a product’s
position within the context of the current marketplace (the
competitive frame) is critically important—sometimes as
important as the product itself. Begin by assessing who the
competitors are and what products they market (the com-
petitive set). This type of benchmarking is imperative to
understanding the competition’s strengths and weaknesses
so you are able to develop a successful and marketable prod-
uct. However, when true innovation is involved, finding simi-
lar products or establishing a competitive set may be difficult.
A well‐developed concept illustrates a product’s key attributes,
c19.indd 399 1/14/2016 7:56:33 AM
400 • CULINOLOGY®
including the consumer’s wants or needs the product is
intended to meet.
Ultimately, ideas should go through a screening process to
cull redundancies, address hurdles such as cost or feasibility,
and eliminate ideas that do not fit manufacturing compe-
tencies. Surviving ideas are then categorized and defended
based on their ability to meet the defined consumer need or
market opportunity.
For example, a few years ago a major food company iden-
tified a need for healthier non‐fried appetizers. The ideation
session yielded several products that potentially met the
need. The defense for one potential product solution, Project
X Flatbreads, follows.
The process started with the articulated consumer needs
derived from the research (Figure 19.3).
FIGURE 19.3 Project X consumer research.
Project X
Consumer Feedback
Concept Rating FSR QSR C‐Store
Consumer H HM M
FSR: Consumers liked that these are new and non‐fried.
FSR consumers are more willing to experiment with
unusual flavor combinations.
• “Appetizers are the same anywhere. They are all unhealthy
and me and my wife don’t even think about ordering them
anymore. I like alternatives to fried foods, I want some.”
• “You have a hard time finding appetizers that aren’t fried
or really greasy.”
QSR: Consumers like the idea of some non‐fried
options in this channel. Consumers like the portability
of the concept and see it as a perfect fit for on‐the‐go
consumption. Flavors will need to be more mainstream
in this channel.
• “It gives me variety that I don’t currently have. These also
seem like they would be perfect for eating in the car.”
• “This is much lighter than a Big Mac or something like
that. Not so sure about the varieties, but the overall
concept is a winner.”
C‐Store: This concept had medium appeal with C‐Store
consumers but could be easily modified to increase
consumer appeal. Consumers liked that these were
healthier than current options but were skeptical about
the product delivering on its claims of taste and ease of
preparation. Flavors should be traditional.
• “I like the idea that it suggests that it is not fried or
unhealthy, because that is a problem. I have a tight
schedule, everyone tries to eat healthy, but you can grab
something in a pinch without damaging your health.”
Source: Craig Julius.
Based on those needs, a product attribute statement was
crafted (Figure 19.4).
A product solution summary then conceptualized a prod-
uct line that addressed the identified needs and opportuni-
ties (Figure 19.5).
Next, an analysis of the competitive landscape was
presented (Figure 19.6)
At some point, this may lead to a specific product proto-
type that is the manifestation of the concept summary; it
could be one or several product ideas that are interpretations
of the consumer need. Each unique concept or prototype
undergoes further testing and validation.
FIGURE 19.4 Project X product attributes.
Project X
Product Fit
Strategic Link
• Out of Home Consumer—Offers consumers a new,
healthier snack and appetizer option.
• Operator—Gives them a “new to the world” selection
of non-fried, quick, easy, and high‐quality snacks and
appetizers.
• Healthier options that leverage current brands and
competencies to meet emerging consumer trends.
Desired Product Attributes
• Temperature consistency and control are vital. We
will need a product that enables operators to deliver
re heated appetizers that are within the required
temperature range, but where the fillings will not be
too scalding.
• Offer a variety of flavors appropriate to each targeted
channel: more traditional flavors for QSR and C‐Store
(broccoli and cheese type offerings) and more exotic
flavors for FSR (ethnic varieties).
• Flavors should be tailored to the menus of restaurant
segments (Mexican, American, etc.).
• Products should have fresh and healthy visual cues,
ex.: grill marks.
• For FSR and QSR, make them look less like hot pockets
and more like quality, unique appetizers (possibly make
the fillings more colorful, unique).
• Consider offering assorted sizes, including a “sandwich
size” for QSR and C‐Store that can be eaten as a full meal.
• These need to be able to deliver non‐microwaved taste
through the microwave.
• Product needs to deliver on cooking promises—can’t
taste soggy or too rough.
• Cannot look like a frozen retail product.
• Needs to be easily heated by C‐Store consumers in the
microwave.
• Needs to be easy to prepare, requiring little BOH skill.
• Manufacturing flexibility to accommodate customization
for large-volume opportunities.
Source: Craig Julius.
c19.indd 400 1/14/2016 7:56:33 AM
CHAPTER 19 • Commercializing the Culinary Gold Standard • 401
FIGURE 19.5 Project X concept summary.
Project X
Concept Summary
A line of non‐fried appetizers/snacks in a variety of interesting flavors that look freshly‐made.
Step 4 Screening Criteria Results Summary
Concept Rating FSR QSR C‐Store
Operator H H H
Consumer H HM M
Revenue
• Greater than $20 million
EBITA
• Greater than 20%
Technical Feasibility
• 2 (of 5)
Prototype Timeline
• Greater than 6 months
Time to Launch
• 12 to 24 months
Source: Craig Julius.
FIGURE 19.6 Project X competitive landscape.
Project X
Competitive Market Prices
Product Case Config. Case Price Price/lb. Price/unit
Cheese Sticks 4/3 lb. $33.64 $2.80/lb.
Stuffed Jalapenos 4/4 lb. $51.85 $3.24/lb.
Breaded Vegetables 6/2.5 lb. $24.27 $1.62/lb.
Stuffed Olives 2/4 lb. $29.42 3.68/lb.
Spring Rolls 2/24/1.5 oz. $29.25 $3.25/lb. $0.30/unit
Egg Rolls 3/20/3 oz. $23.70 $2.11/lb. $0.40/unit
Potstickers 6/30/1 oz. $47.50 $4.22/lb. $0.26/unit
Quesadilla Rolls 4/4 lb. $57.00 $3.56/lb.
Dips 36/6 oz. $40.90 $1.14 $0.19
Category Trends
• Rise in ethnic offerings, both new flavors and mini versions of popular entrees
• Pan‐Asian, Mexican, Fusion
• Mini tacos, quesadillas, tapas, egg/spring rolls, etc.
• Heavy new product focus at leading chains
• Appetizers are the #3 new menu item (behind seafood entrées and desserts).
• New appetizer types: 1. Seafood; 2. Southwest/Mexican; 3. Asian
• Expanded usage as “dippable” meal alternatives for kids and teens
• Growth in “consumer‐friendly” forms
• Boneless wings, poppable shapes, dip injected into the product
• Growing trend toward expanded day‐parts
• Mini pockets at breakfast, lunch sampler trays
Source: Craig Julius.
c19.indd 401 1/14/2016 7:56:34 AM
402 • CULINOLOGY®
Balsamic Vinaigrette:
Three Pathways to Gold
A dressing company desires to launch a balsamic vinaigrette
to extend its current retail line of salad dressings.
Scenario 1—The product development team wishes to
establish a culinary gold standard without current capability
as a restriction. The team creates a balsamic vinaigrette rec-
ipe using top‐quality kitchen ingredients with no considera-
tion of supply chain, cost, or manufacturing feasibility. The
goal is to create the best possible version of the product to
serve as a benchmark against which all commercialized ver-
sions will be compared.
Scenario 2—The culinary team feels they have sufficient
commercial raw materials already in the system to meet their
needs and uses current ingredients to develop the gold
standard dressing. This is a more responsible approach, as
it will be easier to match and scale up as the team moves
forward. Operational constraints, shelf‐life concerns, and
packaging still must be considered and taken into account,
but at least initial ingredient issues are already addressed.
Even though it’s made with commercial ingredients, this is
still a culinary gold standard.
Scenario 3—The competitor is by far the leading brand of
balsamic vinaigrette in the marketplace, so in essence they
have established the gold standard. In this case, the competi-
tor’s product is used as the gold standard and becomes the
benchmark for consumer testing—that is, a new product must
score at parity or outperform the market leader to be accepted.
Regardless of which method is used, the approved product
or recipe should be the reference or benchmark item all the way
through the development and commercialization of a new item.
The goal is to ensure the final commercialized product stays
true to the key attributes that made the gold standard gold.
Collaborative Marketing
Partnering to maximize marketing resources is a synergistic
approach that many of today’s food and beverage companies
use; this approach is also referred to as horizontal marketing.
For example, a salad dressing company may collaborate with a
packaged fresh salad producer to co‐package the dressing as
part of a salad kit for a national retailer. In this case, the dressing
manufacturer can leverage the salad company’s sales, market-
ing, and distribution network to its own advantage while pro-
viding a service or capability (making dressings) the salad
company neither has nor wishes to invest in. The two compa-
nies each focus on their core competencies and collaborate to
bring a convenient meal solution to the consumer.
Market Opportunity
Assessment
To determine the real needs of the consumer, the market
must be analyzed quantitatively and qualitatively. This assess-
ment often determines whether or not the project warrants
the investment of resources and moves forward. Even though
the idea may fit a core competency of the company, the
decision to allocate resources must take into account actual
market needs or wants. Competitive product attributes,
consumer purchasing trends, and socioeconomic factors are
all top areas for investigation.
Using consumer focus groups to gain these insights is
a costly and complex process, but one that increases the
chances for success. Asking consumers what they want, how
they will use it, and how much they are willing to pay for it pro-
vides valuable information to guide the company to successful
product development. Today, concepts can be tested quickly
and inexpensively via online models, and companies often use
online chat technology to replace face‐to‐face focus groups.
Once consumer input has refined and narrowed the
concept options, consumer reaction to actual protocepts
(the chef ’s renditions of the concept) or prototypes (the
early commercialized versions of the protocepts) help
refine the product concept and confirm that it meets the
expectations developed during concept testing. The hard
work of needs assessment, ideation, concept development
and refinement, and preliminary research give the team
the necessary information to develop strong product and
project statements.
Project and Product
Statements
Within the project statement, all the marketing assessment
data is distilled into a concise document that outlines the busi-
ness plan for the internal cross‐functional team that executes
the conceptualization, development, manufacturing, launch,
and eventual management of the new product or product line.
This must be conveyed in context of the company’s strategic
goals, target market, competitive arena, regulatory restraints,
and financial parameters. Associated timeline and departmen-
tal and individual responsibilities are also outlined.
FIGURE 19.7 Balsamic vinaigrette.
Source: Stuart Monk/Getty Images, Inc.
c19.indd 402 1/14/2016 7:56:36 AM
CHAPTER 19 • Commercializing the Culinary Gold Standard • 403
The product statement drills down to the specific attributes
the new product will ideally possess. The challenge here is
that in many cases there are incompatible traits. For instance,
a 45‐day required shelf life for distribution is incompatible
with a bright green herb garnish. Culinary descriptions, pho-
tos, and even videos can all be used to convey the important
and desirable product traits the research and development
(R&D) team is being asked to achieve.
Good product and project statements set up strategic
guardrails that help clarify and define not only what the prod-
uct or project is but also what it is not. These documents help
guide the R&D team in gold standard development.
What Is a Gold Standard, and
How Is It Developed?
Development and approval of a gold standard is a cross‐
functional activity. How the gold standard is developed can
differ from project to project. It is always a benchmark, a point
of reference that future commercialized iterations are judged
against; however, it can be approached in different ways. The
process for defining and using gold standards should be estab-
lished as company protocol, customized to meet the needs
and culture of the company, brand team, or project team.
Gold standard development is the point in the process
where the broad skill set of the Culinology® professional
comes into play to create a physical product, designed to
intentionally and specifically address the attributes identi-
fied in the research and ideation phases. These attributes
include but are not limited to appearance, aroma, flavor, tex-
ture, and even sensory experiences such as spicy heat and
menthol cooling. The gold standard is often confirmed only
after consumer research; in the vinaigrette example, all of the
options—straight kitchen recipe, bench prototype using
commercial ingredients, and the competitive market leader—
could be evaluated by consumers both to capture preference
and to gain input on the attributes that will allow the R&D
team to refine the gold standard to meet as many of the con-
sumer’s needs as possible.
Product and Process
Development
Next, serious commercialization begins. This is the process of
taking a kitchen or bench recipe through the obstacle course
of assessing, understanding, and overcoming manufacturing,
regulatory, food safety, and distribution challenges. A tal-
ented culinary team can put together an amazing product
that’s a delight to the eye and the palate—a true feast for the
senses. It’s when the development team begins to translate
that experience into a product that can be reproduced
consistently—literally millions of times—in a format that can
be consumed months later by someone miles away—that
things get really interesting.
Good development teams work cross‐functionally to con-
sider technical, manufacturing, food safety, procurement, and
Gold Standard Development and
Impact on Commercialization:
Spud Wars—A Real‐Life Experience
A dry, shelf‐stable “just add water and wait 5 minutes” soup
line was expanded to include a line of savory instant mashed
potato products.
Product Line Expectations
• All ingredients must be dry, with less than 5‐minute rehy-
dration time required.
• Particle size is limited due to manufacturing equipment
constraints.
• This line must be all‐natural and vegetarian.
• Sodium content is restricted to less than 500 mg per serving.
• A flavor match to gold standard is critical; the finished
product cannot be similar or equally acceptable but must
match the gold standard flavor profile within narrow limits.
Gold Standard Development
• Fresh kitchen ingredients were used.
• Culinary cooking methods were used, including searing
and braising fresh meat to obtain a savory flavor profile
target for matching using vegetarian ingredients.
• No limits (including on sodium) were imposed on flavor
development.
In the context of the product line expectations, the first
two points in gold standard development could be overcome;
the team had the talent, experience, and ingredient resources
to make the transition from a fresh dish containing meat‐
derived ingredients to a dehydrated vegetarian product.
What could not be overcome was a flavor profile designed
with more than 1200 mg sodium per serving! If the gold
standard had been developed and validated with the sodium
restriction in mind, it would have had a different flavor pro-
file . . . and may well have been preferred by consumers!
FIGURE 19.8 Mashed potatoes.
Source: robynmac/Getty Images, Inc.
c19.indd 403 1/14/2016 7:56:50 AM
404 • CULINOLOGY®
Challenges in Authenticity
in Manufacturing:
A Mediterranean Example
As are most regional and ethnic foods, Mediterranean cui-
sine is typified by specific cooking techniques and ingredi-
ents. One signature “flavor print” is the use of olive oil and
garlic. A common technique involves caramelizing vegeta-
bles, often combined with seasonings, to yield complex fla-
vor combinations such as roasted mirepoix or sofrito (a
classic Spanish or Portuguese sauce composed of aromatic
ingredients such as garlic, onion, paprika, peppers, and
tomatoes cut into small pieces and sauteed or braised in
olive oil). Another example is oil‐simmered vegetable stews
such as ratatouille and caponata.
Adhering to authentic Mediterranean flavor prints in a
commercial kitchen is not a difficult challenge for a trained
chef. However, traditional manufacturing environments, with
their enormous production volumes and massive equipment,
create constraints that typically do not lend themselves well
to traditional cooking techniques. Creating signature flavor
nuances in a manufacturing environment requires experi-
ence, knowledge, and the ability to meet challenges through
innovative thinking—in other words, Culinology® in action!
Two Examples: Olive Oil and Garlic Flavors
Many manufacturers use vegetable oil instead of costlier olive
oil, or they use a third‐press (solvent‐extracted) olive oil. These
choices may be made due to cost considerations, commercial
ingredient availability, or even processing constraints, such as
the need for an oil with a higher smoke point due to the manu-
facturing process. Excellent olive oil flavors are available to
add back authenticity to the product. Even a low‐fat product
can exhibit a fruity olive oil flavor, or flavors can be used to
boost that of cheaper, more cost‐effective oils.
When garlic is commercially processed, its aromatic qual-
ities are often lost, or objectionable off flavors, often metallic
in nature, develop. Sometimes a really good garlic flavor or
oleoresin is a better formula option than garlic pastes or
other commercially processed garlic choices.
FIGURE 19.9 Caponata.
Source: travellinglight/Getty Images, Inc.
These are just a couple of ways that authenticity can be
reconstructed to maintain gold‐standard flavor integrity in
the commercialized product.
Cooking Techniques
The traditional cooking techniques used to create authentic
tastes, textures, and appearances are one of the most com-
monly compromised areas in food manufacturing. The effect
on foods is a loss of gold‐standard integrity. In traditional
manufacturing environments, especially if the plant is more
than a decade old, equipment is expected only to mix, cook,
and deposit. Speed is everything. “Authenticity is for restau-
rant chefs,” as one plant engineer used to say (ironically, he
left the food business for the automotive industry).
Recently, high‐tech manufacturing equipment has come
to market that provides the high-heat and high-contact sur-
faces necessary to duplicate authentic cooking techniques.
In an effort to automate the Chinese stir‐fry process, one
company recreated the manual stir‐frying technique used for
centuries in preparing Chinese dishes and incorporated it into
an automated mechanical process. A hot thermal oil system
was designed to provide high temperatures (500°F [260°C])
at low pressure to work in conjunction with a mechanical
stir‐fry device. The machine mechanically stir‐fries food on a
very large scale while retaining hand‐stir‐fried product quality.
Applications for this machine go beyond Asian stir‐fry. The
high heat allows for true caramelization and complex flavor
creation; it can be effectively used to replicate the color and
flavor of Mediterranean cuisines as well. In contrast, traditional
steam‐kettle cooking is a low‐temperature (212°F [100°C]),
moist‐heat process. The resulting soggy texture is caused by
the stewing of product in its own juices.
To achieve the qualities of dry‐heat roasting, another com-
pany makes high‐heat in‐line and spiral equipment that pro-
duces great results for roasting meats and vegetables. They
also make an in‐line fire roaster—that is, a flame broiler—that
can impart charring and varying degrees of caramelization to
meats and vegetables. Trolley cookers and cavern ovens, long
used in the meat cooking and smoking industry, can also pro-
vide dry-heat alternatives to traditional steam-kettle cooking.
regulatory feasibility as part of the process from the original
concept forward. A good gold standard not only represents
the eating quality of the final commercialized product but is
also at least moderately practical. In the Spud Wars example,
the gold standard for a new savory mashed potato lunch line
was developed by a culinary‐based test kitchen team in one
location and commercialized by a lab and production team
in another location. Product‐line restrictions were not con-
sidered during gold standard development to avoid limiting
the creativity of the test kitchen team. Using fresh ingredients
and even meat (to provide a savory flavor target even though
the end product is vegetarian) were hurdles that could have
been overcome. However, the decision not to limit the creativity
of the culinary team allowed them to create an unrealistic
c19.indd 404 1/14/2016 7:57:07 AM
CHAPTER 19 • Commercializing the Culinary Gold Standard • 405
target with too much salt for the R&D team to match at the
required sodium level. The finished product was commer-
cialized and was successful in every way except two: It didn’t
deliver on flavor, and it failed in the marketplace. This was
not the fault of either the culinary team or the commerciali-
zation team; it was a failure of the entire cross‐functional pro-
cess. Lack of integration between the development teams
caused the product line to fail.
One of the most important issues any commercialization
team must understand is the hierarchy of priority in decision
Ingredient Considerations:
The Case of the Marinara Sauce
Imagine you are working on a marinara sauce. The gold
standard was kitchen‐made using fresh ingredients, from
the tomatoes to the herbs. The savory flavor character came
from browning fresh Italian sausage, using the fat and fond
(browned material at the bottom of the pan left from sautéing
the meat) as a base for sautéing the aromatics (in this case,
onion, garlic, and fresh herbs), and the sauce was built from
there and finished with a splash of red wine in the late stages
of cooking. Here are some of the questions to consider in
commercialization:
• How will the finished sauce be distributed and sold? Will it
be shelf‐stable (jar, can, or flexible pouch), refrigerated, or
frozen? The perfect ingredient choice for a frozen sauce
may not survive thermal processing, for instance.
• Can processed tomato ingredients such as diced, puree, or
paste replace fresh tomatoes?
• Is an industrial flavor available to help replace the flavor of
the sausage fat and fond? In some cases, a low‐calorie or
even vegetarian sauce may be desired, but with the savory
flavor notes that came from browning the seasoned meat
in the gold standard.
• What combination of commercial ingredients might replace
fresh sautéed aromatics? Frozen sautéed aromatics are
possible, but the best answer is more likely to take the form
of culinary base or concentrate, frozen puree, drum‐dried
or spray‐dried ingredients, or a flavoring—or a combination
of two or more of these choices.
• Will dry herbs provide the needed flavor? Are industrial fla-
vors required to boost the fresh flavor notes? Will IQF herbs
or a fresh‐frozen puree meet the cost target?
• Must wine be used to finish the sauce? Will a commercial
flavor, dealcoholized wine, or wine concentrate do the job?
• Consider the sauce’s mouthfeel and cling. Are functional
solutions such as starches or gums needed to match the
original texture?
• Will the flavor, texture, appearance, and aroma survive the
temperature fluctuations of distribution, including potential
abuse by the end user? Does the product need color or flavor
protection? Will syneresis (separation) control be an issue?
• What else could go wrong, and how can the product be
protected against it?
making. Compromises are required as part of the commer-
cialization process, and the team must agree from the begin-
ning what can and cannot be compromised on. Are we willing
to give up flavor, texture, or eating quality for cost? Give up
shelf‐life or distribution assumptions for eating quality? Give
up texture or appearance for manufacturing efficiency or dis-
tribution capability? The list can seem endless and is unique
to every product, but it must be confronted.
One of the first things a commercialization team does is
begin to transition a kitchen recipe into a manufacturing for-
mula, including translating kitchen or culinary ingredients
and measurements (such as cups or teaspoons) to commer-
cial ingredients and measurements (such as percentages or
pounds per batch). During this phase a second standard,
sometimes called the manufacturing standard, is developed;
it may differ slightly from the culinary gold standard, but it
incorporates all of the necessary commercialization trade‐
offs the team has agreed on (and does not diverge from those
considered non‐negotiable). Both the culinary and manufac-
turing gold standards are used as benchmarks during evalua-
tion of the products produced at every phase of development
and commercialization testing. It’s common to require that a
manufacturing standard scores near parity compared to the
original gold standard in consumer testing to ensure the con-
sumer needs are still being met.
Examples of commercial ingredients include:
• Familiar kitchen staples such as butter or cheese in
50‐pound blocks and tomato paste in 55‐gallon drums
instead of little tin cans.
• Commercial flavorings that replicate the savory deli-
ciousness developed in the kitchen via cooking, such as
sautéing, searing, roasting, or braising; it may be imprac-
tical if not impossible to duplicate these cooking pro-
cesses on a mass scale using industrial equipment.
• Functional ingredients, such as heat‐ and acid‐resistant
starches, that can help the product survive mass pro-
duction, commercial packaging and distribution, and
months of storage—yet still eat as good as kitchen fresh
at the time of consumption.
Cost is a big part of product development. Material costs
such as ingredients, packaging, and other material inputs, and
manufacturing costs such as labor, overhead, and efficiency,
must all be considered; cost of goods sold (COGS) is a key
input. Food safety, sanitation, and Hazard Analysis and Critical
Control Point (HACCP) requirements as well as the safety
of production workers must also be taken into account.
One example of worker safety concerns is the highly publi-
cized case some years ago of lung disease in unprotected
popcorn plant workers. The workers were exposed to the
flavor chemical diacetyl, a component of the butter flavor
then used in microwave popcorn. Another example is the
ban many manufacturing plants put on ingredients packed in
metal cans (opening the can may produce metal shavings)
or glass containers (due to the chance of breakage and
subsequent glass shards). Both metal shavings and glass
c19.indd 405 1/14/2016 7:57:07 AM
406 • CULINOLOGY®
filling operations in the manufacturing process. These issues
can be overcome by specifying larger dices that will be sized
just right after moving through the production process.
Sauces may be prone to syneresis during storage and
distribution, and functional ingredients such as acid‐stable
starches or gums may be needed to protect against it. Now
is the time to think ahead to what can go wrong and build
in defenses. Because functional ingredients such as starches
and gums can mask flavor, the recipe may need tweaking
to come back to the desired flavor impact after other quality
issues have been addressed.
When a bench formula or manufacturing standard is
complete and approved, it’s a good idea to do some quick
disaster checking. Consider what can go wrong and see what
Blowing Hot and Cold:
The Effect of Frozen Vegetables
in a Real‐Life Situation
A common tactic in production is to take advantage of IQF
components to help cool a sauce quickly. For instance, if
you are making chicken à la king with a cook‐up sauce, you
might cook the sauce and then add the IQF vegetables to
quickly cool it for HACCP reasons.
You have planned ahead and designed your sauce con-
sistency to be just right once the vegetables thaw and the
blend equilibrates to <40°F (4°C). The manufacturing speci-
fications are carefully crafted to the final degree, clearly stat-
ing the correct temperature of the sauce, the temperature
of the vegetables, and the right time to blend them together.
What could go wrong with that?
Consider this: The vegetables are assumed (even speci-
fied) to be at 30°F (–1°C), the temperature of the holding
freezer in the plant. However, yesterday someone forgot to
order more vegetables into the plant from off‐site cold stor-
age. Everyone scrambled, and the vegetables are delivered
just in time—but they are at –5°F (–20°C) because they just
came in on a freezer truck! A thick, hot sauce designed per-
fectly for 30°F (–1°C) vegetables is likely to bind up solid
when hit with this temperature load. Mixing and pumping are
not in the cards until the vegetables temper for a few hours.
Conversely, consider this scenario: The vegetables were
pulled out of the holding freezer right on time this morning
for the first batch of sauce—and a key piece of equipment
failed. In the scramble to get production up and running, no
one remembered to put the vegetables away. By the time the
next batch of hot sauce is ready for vegetables, they are up
to 55°F (13°C). Now you have a loose, watery sauce that
does not cool fast enough to meet HACCP guidelines, and
the vegetables are warm and limp—completely unable to pass
through the subsequent blending and pumping processes in
one piece.
It’s surprising how a relatively minor problem can throw
an entire processing schedule out of whack and potentially
ruin a large batch of product.
shards are hazards to production workers and, if they get
into the product, to consumers.
The most practical way to transition from kitchen recipe to
production formula is to investigate the impact of each ingre-
dient change one at a time. It’s important to fully understand
how each of the ingredients and processing steps affects the
finished product; only then can the developer reconstruct the
product as a commercial manufacturing‐ready formula that
delivers the same key attributes as the gold standard.
Good developers have a commercialization toolbox at
the ready, including a clear understanding of industrial flavor
and functional ingredients and solid experience with manu-
facturing processes. A key tool is the expertise of applica-
tions and culinary resources provided by ingredient and
equipment suppliers. Many flavor houses provide culinary
flavors that replicate or enhance the flavors obtained by
different cooking methods in the kitchen. Vegetable suppliers
offer ready‐cut, pre‐cooked, individually quick‐frozen (IQF)
vegetables—seared, grilled, roasted, and seasoned. Herbs
can be obtained IQF as fresh‐frozen purees, culinary bases,
extracts, flavorings, and, of course, dry (including freeze‐
dried). Nearly every imaginable commercial need can be
filled if it is clearly identified.
Just as in the kitchen, specifications—dice size of ingre-
dients, defined unit processes such as mixing, cooking, and
order of ingredient addition, and so on—are key manufactur-
ing steps that affect the finished product considerably. Only
through familiarity with commercial ingredients and manu-
facturing equipment can the developer understand them
well enough to apply them appropriately.
Consider one at a time the ingredients and methods used
to make a savory marinara sauce. Starting with the tomatoes,
can commercial tomato products (available in 55‐gallon
drums or pallet‐sized bag‐in‐box technology) replace the
taste and texture of cooked‐from‐fresh tomatoes? Once this
question is resolved, the team can move on to background
flavors such as the meaty and savory notes, the aromatics, the
herb notes, and the wine component. Frequent reference to
the gold standard helps the team avoid making small step-
wise changes from version to version that can take the final
formula quite far from the original target flavor profile. During
the flavor development process, it is common to note for later
attention—but not attempt to solve yet—small texture, color,
and other attributes that may also begin to stray from the
gold standard. Major texture attributes should be addressed
immediately—for example, how the decision to use a tomato
combination including diced, crushed, pureed, or paste for
texture and appearance also affects flavor and mouthfeel. But
minor, correctable attributes such as viscosity can be dealt
with later. Understanding the difference is where the experi-
ence and expertise of the Culinology® professional comes in.
Once the flavor profile match is achieved, it’s time to correct
minor appearance and textural attributes such as viscosity,
pulpiness, or color hue and intensity, again always referring
to the gold standard. It’s important to look ahead to
manufacturing constraints during this step—for example, many
particulates are reduced in size during mixing, pumping, and
c19.indd 406 1/14/2016 7:57:07 AM
CHAPTER 19 • Commercializing the Culinary Gold Standard • 407
happens when it does. “What can go wrong” includes investi-
gating the limits of every unit process—basically trying to
break a system or product—all the way through processing
and packaging. Not only product quality but food safety must
be considered. Examples include:
• Over‐ or under‐processing—What happens to the prod-
uct if ingredient weighing, cutting, blending, cooking,
and other unit processes are not well controlled? Testing
each area, including intentional process variations, can
determine how tightly the manufacturing process speci-
fications must be set for each unit process.
• Work‐in‐process (WIP) components—What if frozen ingre-
dients are thawed too far or held too long after thawing—
or not thawed enough? What are the options if a key piece
of equipment fails mid‐process? How can WIP materials
be protected or recovered? Are there components pro-
duced in one area or facility and used later in another?
What are the packaging, distribution, and shelf‐life limits
of these intermediate items, and how will finished product
quality and shelf life be affected by using WIP ingredients
that are approaching end‐of‐life?
Other “defensive” questions that should be considered:
• Are alternate processes or equipment available that
yield the same result? For instance, what if Plant A uses
a chopper for particle reduction and Plant B uses a
grinder? Can the product be made in both plants suc-
cessfully, in both cases yielding a finished product that
gives the end consumer the same experience?
• Does current packaging technology meet the need for
the new product? Products are often designed around
current packaging capabilities, but sometimes new
packaging technologies are necessary to deliver on the
concept; packaging is a critical component of product
design, and it is often overlooked.
It is only by understanding the strengths and limitations
of key manufacturing equipment and unit operations, just as
one must understand the kitchen gold standard, that robust
manufacturing specifications can be developed and identi-
fied—and it’s only by understanding where to find the edge of
the cliff that one can build the guardrails that allow a team to
create cutting‐edge products that can be produced over and
over, day in and day out, without concern for quality or safety.
Process capability—the inherent variability that cannot be
specified out of a unit process—is a key consideration for
every development and commercialization team. It’s a
common, yet major, mistake to design a product with closer
tolerances than the process is capable of.
Once potential manufacturing abuse is defined and
resolved, distribution abuse must be considered. For example,
distribution abuse for a frozen meal product can be approxi-
mated by running it through a freeze‐thaw abuse series—that
is, freezing the product completely, then letting it thaw at
room temperature completely, then repeating the process.
The results of four or five freeze‐thaw cycles will give a good
indication of potential “real life” challenges.
FIGURE 19.10 A four‐cycle freeze‐thaw protocol.
Cycle 1 Cycle 2 Cycle 3 Cycle 4
Control keep
frozen
keep
frozen
keep
frozen
keep
frozen
Sample 1 thaw,
refreeze
keep
frozen
keep
frozen
keep
frozen
Sample 2 thaw,
refreeze
thaw,
refreeze
keep
frozen
keep
frozen
Sample 3 thaw,
refreeze
thaw,
refreeze
thaw,
refreeze
keep
frozen
Sample 4 thaw,
refreeze
thaw,
refreeze
thaw,
refreeze
thaw,
refreeze
Once the final freeze‐thaw cycle is complete, all five sam-
ples are evaluated side by side against the gold standard and
the manufacturing standard to understand how well the
product withstands the abuse. It’s important at this point to
test the product in final or near‐final packaging and prepare
it for eating the same way a consumer or customer would.
For a retail frozen entrée, this may include preparation in
both a conventional oven and a microwave, for example. For
a foodservice item, it may include time in a steam table. The
product’s performance must be evaluated under the condi-
tions the end user will experience to ensure your product and
package deliver to expectations.
Using the marinara sauce example, it wouldn’t be unusual
to see the product begin to show some syneresis (weeping) at
freeze‐thaw cycle 2, color breakdown (red becoming orange)
by cycle 3, and complete product deterioration beginning
in cycle 4. Most frozen red sauces wouldn’t be considered
acceptable after four or five cycles without functional ingre-
dient support such as color technology to help retain the red
color, specialty starches or hydrocolloids to protect against
syneresis, and added flavor technology to help maintain fresh‐
made taste even after repeated abuse.
Why assume repeated abuse? Consider this scenario: A
pallet of frozen product leaves the production facility via
truck. The truck’s compressor breaks down or is inadequate
for the summer temperatures outside, and the product is a
little thawed when it arrives at the frozen distribution center.
Or the product is delivered to the distribution center in per-
fect condition, but the center has an inattentive employee or
an equipment issue or a scheduling error, and the pallet sits
on the unrefrigerated dock for several hours before being put
in the warehouse freezer. Or the warehouse freezer’s thermo-
stat may be old and inefficient, or set improperly, so the stor-
age temperature exceeds the optimal temperature. Events
such as this can and do happen multiple times between the
production facility and the final grocery store or restaurant
freezer as the truckload is broken down onto pallets and then
cases as it’s moved through picking centers in a distribution
cycle. Everyone has seen grocery freezer aisles full of product
waiting to be put away in the display freezers—another
common example of temperature abuse.
c19.indd 407 1/14/2016 7:57:07 AM
408 • CULINOLOGY®
Finally, the end user often abuses the product. The shop-
per who buys the product may not go straight home and
immediately place it in the home freezer (which may or may
not be set to the correct temperature). Or a foodservice
worker might pull the product out of the restaurant freezer
and let it thaw before he decides he doesn’t need it today and
puts it back. It’s entirely likely that any frozen product sees as
many as six partial or complete freeze‐thaw cycles between
manufacturing and end use. (Note: For unrefrigerated shelf‐
stable products, high‐temperature abuse testing in “hot
boxes” [heated storage rooms] is often used instead of freeze‐
thaw abuse.)
Abuse testing is simply a way to quickly assess how well a
product is likely to hold up under its unique conditions of
manufacturing, distribution, and end use. It can also be a
quick way to approximate what products may look like at the
end of estimated shelf life and is often used as a shelf‐life pre-
diction tool. No matter how good your product is when first
produced, the final consumer won’t evaluate it, and your
brand, until after it is prepared and consumed. By that time,
a multitude of deleterious changes may have occurred. Good
commercialization teams understand the conditions their
product is likely to experience between manufacturing and
consumption and build in the means for the product to sur-
vive this reality.
Back to our manufacturing standard: If the product can-
not stand up to abuse, the formula must be modified before
going for plant trial or pilot testing. Bench development is
significantly less expensive than production testing, partly
because less product is needed for testing and partly because
of the cost of manufacturing line time. Every disruption of a
plant’s normal production schedule can be costly.
Developing Specifications
In preparation for plant or pilot testing, detailed processing
specifications must be developed to ensure the manufactur-
ing team has the information in hand to allow them to con-
sistently reproduce the right product time after time after
time. Appropriate documentation includes all inputs—raw
material specifications, production recipe or formula, and
detailed processing and handling instructions for each step
from raw material through finished pallet. It covers estimated
plant labor required, expected run rate (time and yield),
HACCP and allergen-management plans, proposed contain-
ers (cans, jars, pouches, and so on), net fill requirements,
outer packaging (bands, sleeves, corrugated, etc.), all the way
up to pallet patterns and pallet labeling requirements. It often
specifies not only the equipment to use but also the equip-
ment settings for that product. Good manufacturers typically
have fill‐in‐the‐blank forms where all of the information required
for manufacturing each product can be recorded, so develop-
ing process specifications is not as intimidating as it sounds.
Preliminary specifications should be confirmed or updated
during production trials; it’s common to find surprises in full
production testing, and all of the adjustments made during
testing must be captured in the final specifications.
Once the product is finalized, a finished product specifica-
tion is written that clearly defines the outcomes desired. Then
the material and processing specifications for achieving those
outcomes are written. Key product characteristics must be
measurable without human bias; examples may include color,
texture, viscosity, pH, degrees Brix, particulate or piece size, net
weight (and component weights), and any other measures rel-
evant to a particular product. Photos of the finished product in
its container, with and without the label, should also be
included so that production personnel can refer back to what
the packaged product should (and should not) look like after fill-
ing and after labeling. Even flavor can be measured, and should
be, even if by simply comparing to a bench‐ or kitchen‐produced
gold standard on a regular basis. Another option is to use a
trained sensory panel for unbiased flavor evaluation; a good
trained panel is an effective and reproducible measuring tool.
Every unit process from start to finish must be considered.
Here is an example list of questions to consider while think-
ing through the processing assumptions:
• Which production facility? What equipment is available
and should be used? Sketch out the assumed production
processes, including the HACCP plan.
• Does the product have a hold step between batching
and cooking? What are the minimum and maximum
hold times and required temperature conditions for qual-
ity and food safety?
• Does the product move from plant to plant between unit
processes, such as between batching and cooking, or
between cooking and final packaging? What is necessary
to protect the integrity of the product during this time?
• What internal control systems must be used (such
as labels and bin tags as well as internal WIP docu-
mentation) to manage the WIP materials awaiting final
processing?
• What happens if a procedure is not followed? Is the
product compromised in quality, safety, or functionality?
What contingencies are available?
• Can WIP or finished components that do not meet all
specifications be reworked into subsequent production?
Under what conditions? If not, what are the disposal
protocols?
• What are the key unit analyses that confirm the process
is properly controlled (for example, nutritional analysis,
microbial analysis, time or temperature checks, color
evaluation, packaging evaluation, manufacturing records
and checkpoints)? What are the sampling plans and doc-
umentation protocols for each?
It is the work of the cross‐functional team, at this point
relying heavily on manufacturing, engineering, and quality
assurance professionals, to think through all of the relevant
questions for a given project; there are many more than the
short list above.
Normally, at this stage the documentation is considered
preliminary; specifications are not finalized until sufficient
c19.indd 408 1/14/2016 7:57:07 AM
CHAPTER 19 • Commercializing the Culinary Gold Standard • 409
The Cheesecake Chronicles:
A Real‐Life Experience
An unbaked, single‐serve, frozen cheesecake was devel-
oped for retail distribution. It had a cream cheese base held
together with a combination of modified food starch and
gelatin. The product was designed to be mixed in 500‐pound
batches, pumped to a filler, dispensed into a single‐serve
package, sealed with a clear plastic film, and frozen before
being packed in a paperboard sleeve and cardboard case. At
the time, this was novel technology. It took about six months
of experimentation and plant testing (literally hundreds of
plant test batches) to achieve the goal and match the
approved gold standard and manufacturing standard.
The product was successful. Soon a capacity increase
was needed, and a new 1000‐pound mixer was installed.
The developer had to return to the plant and retool both the
formula and the process to achieve the same finished prod-
uct using the new mixing equipment.
A year later, as part of the plant’s HACCP process, a wall
was built between the batching area and the filling line to
separate raw materials from the finished‐goods packaging
area. As part of this change, the mixer was moved 10 feet
away from the filler; this time both the plumbing and the
formula needed updating. The additional shear created by
pumping the product through an additional 10 feet of stain-
less‐steel piping completely changed the finished texture. A
larger‐diameter pipe and slight adjustments in the textural
components of the formula solved the problem.
Seemingly innocuous processing changes can seriously
affect the attributes and acceptability of a finished product.
manufacturing experience is gained to validate them. Even a
successful production trial won’t uncover all of the manu-
facturing challenges and thus the modifications that will be
required. Only after every aspect of the product has been
considered, tested, and evaluated is the product ready for
scale‐up and launch.
Scale‐Up Testing
When a bench formula is complete, it must be tested in
the production environment, because large‐scale processing
conditions can and will affect the color, flavor, and texture
of the finished product (see sidebar). Production scale‐up
protocols depend on the size of the company; smaller com-
panies may move straight from the lab to the manufacturing
plant, while very large companies generally have pilot facili-
ties (which may be larger than a small manufacturing plant!)
and thus move from bench to pilot plant and then to full
manufacturing.
Scaling a recipe up from “serves 8” to “serves 40” is one
thing, but scaling to hundreds or thousands of pounds per
batch (or, for continuous processes, per hour) can have large
and often unforeseen consequences. Many of these can be
prevented or mitigated by doing intermediate‐scale, sequen-
tial batch testing. Based on internal evaluation (cost targets,
desired shelf life, adjustment of flavor attributes) and external
requirements (customer requests or consumer panel feed-
back), the R&D team may go back to the bench during the
scale‐up process to use their culinary prowess, scientific
knowledge, and process technology experience to reengineer
the product to meet all desired requirements.
The finished products produced at every scale‐up test
must be evaluated against both the original gold standard
and the approved manufacturing standard, and any observed
differences resolved by tweaking the formula, the process-
ing conditions, or both. The product is then retested in
the manufacturing environment to ensure success. A new
product is not ready for introduction until all of the manu-
facturing bugs are identified and resolved to the satis-
faction of the entire cross‐functional team, and all of the
documentation and specifications are updated to reflect
the scale‐up experience. Final specifications are generally
validated during or even following product launch, when
sufficient volume of manufacturing occurs to confirm that
specs are sufficient and complete for full manufacturing on
a daily basis.
Product Launch
Market Testing
The concept is developed, the R&D process is complete,
specifications are set, full production runs are made, and
the supporting marketing program is developed. Now what?
The final stage is to test the food or beverage item in the
marketplace, in real‐life scenarios, with actual consumers
and all the innate variables to which the item is subjected
as it rolls out.
FIGURE 19.11 Cheesecake production.
Source: AdShooter/Getty Images, Inc.
c19.indd 409 1/14/2016 7:57:25 AM
410 • CULINOLOGY®
Lettuce Wrap Recollections:
A Real‐Life Experience
A stir‐fry sauce was developed for a casual‐dining restau-
rant chain’s lettuce wrap. It was first made in 1000‐gram
batches of sauce and tested in a wok to see if the flavor was
right. Yes, the garlic came through fine, and the flavor bal-
ance was great. Did the viscosity hold up to the heat abuse
on the stove? Sure; when the sauce was added to the
cooked protein and vegetables, it bound everything together
beautifully.
Then the team considered what would happen to the fill-
ing as it sat during the estimated time it would take to get to
the guest. No problem here; the sauce still had a silky texture
yet was thick enough to bind everything together in the let-
tuce leaf and provide a not‐too‐messy eating experience.
Everything seemed to be right on track—until, that is, the
team made a full production run, pouched the sauces, froze
them for distribution, and sent them on their way for a
regional market test. The product went through the entire
distribution cycle without a hitch. What did change was the
product performance at the restaurant level. The sauce had
been tested on a six‐burner range with a small 14‐inch wok;
the customer used specially‐designed high‐BTU burners,
and their woks were wider and shallower. This changed
everything. The higher heat and shallow vessels caused
faster evaporation, and the sauce therefore began to char on
the edges, to the extent that a burnt flavor was created.
The team went back to the bench. The sauce was thinned
to compensate for evaporation, and the sugar content was
reduced so the sauce wouldn’t be so quick to burn. These
small changes not only put the product back on track,
they also reduced ingredient cost per ounce—a real win‐win
outcome.
intelligence, a systematic data gathering program should
be in place. Guest feedback forms, online queries, and cas-
ual conversations with consumers can all be used during
market testing to validate that the product execution
matches with consumer desires. Once proven in the limited
marketplace, products can be expanded system‐wide until
all relevant locations are in full swing. A benefit of starting
small with regional market testing is the ability to adjust
the product—or even remove it from the market for rework-
ing and retesting—while in small‐scale distribution, thus
improving the odds of a winner once it expands to full
distribution.
Especially relevant to foodservice, the actual product per-
formance must be tested at the restaurant level—or rather
retested, as the bench samples and plant trial production
runs should have already been tested in a real‐life situation.
Testing the functionality of the item within the confines of
the end user’s setting is a critical success factor.
Product Rollout
The culmination of all of this work is a product rollout, filling
all potential channels of trade and points of distribution. For
a retail product, this could include moving from a single
retailer to multiple retailers, from a single region to national
distribution, and from one class of trade (such as grocery)
into multiple classes of trade (such as club, convenience,
mass merchandise, and drugstore). For a foodservice prod-
uct, the transition may be similar; instead of retail classes,
think restaurant chains and foodservice segments.
Financial Evaluation:
An Ongoing Exercise
The financial evaluation of a project is an ongoing process.
Even before a Culinology® professional gets going in the R&D
kitchen, a paper exercise of theoretical costs should be devel-
oped as part of the feasibility determination. Ingredient cost,
labor, overhead, sales and marketing costs, promotional pro-
grams, and other factors that affect the final cost of goods sold
during every stage of the development process must be con-
sidered. As the project moves through development, financial
evaluation must be repeated to ensure the product continues
to meet cost targets. Something as simple as a yield percent-
age variation, a small increase in labor cost (or reduction in
production efficiency), an ingredient cost variation, a different
overhead allocation than expected, or any number of other
factors can change the financial equation and make the differ-
ence between commercial success and failure.
Let’s say your customer, a restaurant chain, determines
that within their system they can afford 9 cents per ounce for
a pasta sauce, a non‐negotiable requirement. A quick cost
evaluation shows your operation can produce the sauce for
around 12 cents per ounce. While 3 cents doesn’t seem like
very much, here it is 25 percent over the customer’s cost
expectation and may be too great a hurdle to overcome—you
have lost the business opportunity. Alternatively, a cross‐
functional team may be able to develop a solution by way of
FIGURE 19.12 Lettuce wrap.
Source: Jaimie Duplass/Getty Images, Inc.
One approach begins with a test market, usually in one
or more geographic regions, often based on the brand’s typi-
cal demographic profile. This approach can work with
either retail or foodservice products. As part of launch
c19.indd 410 1/14/2016 7:57:39 AM
CHAPTER 19 • Commercializing the Culinary Gold Standard • 411
ingredient or formula cost reduction, improved supply chain
practices, better production efficiency, or other solutions to
get the cost in line. It’s important, however, to keep in mind
that solutions must be win‐win: Both the vendor and the cus-
tomer must be satisfied with both the product and the cost.
Sometimes it’s better to say no to an opportunity than to say
yes and then fail to deliver the right product for the right cost.
This sort of failure can spoil a good business relationship and
end up costing far more than the loss of the opportunity.
Post‐Launch Evaluation
The post‐launch evaluation (PLE) is a critical component
of the development process that is often underutilized or
overlooked altogether; even large companies often forego the
PLE. But committing to a PLE and analyzing the results
objectively yields valuable information. Typically the PLE
occurs at 12, 24, and 52 weeks after launch; another way to
determine checkpoints is at set production quantities
(pounds produced and sold) or sales levels (dollars worth of
sales). Here are typical questions asked:
• How is the new product or product line selling?
• Is it meeting projections and expectations? Why or
why not? Were the expectations realistic? Was it
given enough time to realize success?
• Is it being accepted by consumers and operators? Is
it gaining momentum? What are the trial and repeat
sales metrics?
• Does it meet the financial expectations set during
the project? Did it fulfill the original vision? Or did
something happen along the development path that
deviated from the original vision? Was the deviation
defensible?
• Is the marketing message on target? Does it reach the
right consumers or customers?
• Does the sales force understand the product or line?
Are they able to sell it in (gain distribution) to the
right channels of trade?
• If the product is not performing up to expectations, can
it be saved? What other options are available?
• What can we learn?
• If the product or product line is successful, how can
we replicate the success in the future?
• If the product or product line failed, how can we pre-
vent that from happening the next time? Why did it
fail? What did we learn from our mistakes? How can
we improve the process going forward?
Product Optimization
The product optimization process should be continuous
throughout any given product’s life cycle. Generally speaking,
it is an ongoing analysis and continuous improvement in both
formula and manufacturing process, but it may also include
changes in marketing strategy, finance, procurement, and
supply chain, yielding a better, more affordable product and
process. Many companies adhere to a continuous improve-
ment philosophy, but diligent care must be taken to protect
and maintain a product’s original winning attributes such as
flavor, texture, color, and convenience.
In recent years, the term product optimization has some-
times been used to mean “we need to squeeze more money
out of this product”; sometimes a company must protect
or improve margins but is afraid to take a price increase. A
common tactic is to substitute cheaper ingredients in the
formula, thereby reducing costs for improved profit. This
tactic is usually done in stages over a period of months
or even years. Over time, the end product may bear little
resemblance to the original gold standard or manufacturing
standard. If the product eventually fails to live up to customer
or consumer expectations, the result is often late‐stage failure,
especially if a competitor’s product is increasingly perceived
as being superior or offering better value.
However, not all product optimization is a bad thing. In
fact, there are many valid and compelling reasons to opti-
mize, including considerations such as:
• Improvements directed by the PLE
• Competitive pressures
• Cultural change
• New technology or packaging
• Product life cycle: process and formula improvement
Improvements Directed by PLE
Through a post‐launch evaluation, valuable information is
generated that may guide product optimization. For exam-
ple, a company may find that a certain segment of consumers
doesn’t initially understand the value proposition of a prod-
uct, while others have adopted it and are enthusiastic about
its benefits. The marketing team can then take steps to better
convey the message.
One example of the benefit of PLE: A company had
launched a 35‐pound box of rice for the foodservice market at
a rock‐bottom price. The product sold well for the first few
months, but then sales fell dramatically. The PLE revealed
that the cardboard packaging was breaking down. In the
northern half of the United States, the winter months intro-
duce levels of high humidity and snow ; the particular card-
board used for the box was absorbing this moisture, causing
the packaging to lose its structural integrity, breaking apart,
and causing rice to go everywhere. Operators had stopped
buying the product because of this problem. A new, stronger
box with a wax coating solved the problem, and sales gradu-
ally began to improve.
Competitive Pressures
Every successful product is eventually faced with competitive
products that have some added benefit such as lower cost,
longer shelf life, better packaging, or trendy flavors that
compete for market share. Every company is challenged
to continually evaluate its products to ensure they remain
c19.indd 411 1/14/2016 7:57:39 AM
412 • CULINOLOGY®
relevant to the marketplace. Stealing and protecting market
share are two constantly shifting dynamics that lead to product
optimization.
Ongoing competitive intelligence is as critical after launch
as it was in setting the competitive frame for the developing
product. There are distinct approaches to assessing the retail
landscape, foodservice suppliers, and other co‐manufactur-
ers; the retail marketplace is much more structured, and con-
sumer purchase data is easily available. The digital world and
employment of universal product codes (UPCs) has enabled
the capture of retail consumer habits and buying trends—
including what they buy, when, and with what other items—
and all of this is available from specialized data collectors
such as IRI and Nielsen. Foodservice or institutional infor-
mation that is unavailable through this approach can often
be found via restaurant visits, trade show attendance, and
Internet‐based research.
Impact of Economic and
Cultural Change
In recent years, private-label store-brand products have been
taking market share from the national brands. These products
typically have similar attributes to branded products but sell
at a lower price, and in some cases they are preferentially
stocked by store chains, leaving less shelf space for the
national brands to compete for. When faced with economic
uncertainty, some consumers will trade down in price,
and with the recent improvements in store-brand product
and packaging quality, many don’t trade back when times
get better. Private-label manufacturers are typically lean
operations and don’t have expensive corporate marketing
and support staffs to fund, so their cost structure is very
different. Add the willingness of store chains to market their
own brands, and a branded company is faced with finding
new ways to win and retain market share, consumer trust,
and customer loyalty. Focusing on hard‐to‐duplicate product
quality, corporate reputation and brand recognition, devel-
opment of innovative new technologies and packaging, and
large investments in advertising are some of the methods
used to combat store brands.
External forces from cultural change may also exert game‐
changing pressures. The green movement is a global change
that has been gaining supporters for some years. Companies
that wish to compete in this arena are forced to develop and
modify products and packaging to make them more eco‐
friendly or sustainable. The movements toward all‐natural,
organic, non-GMO, sustainable, local or Fair Trade, reduced‐
sodium, gluten‐free, and zero trans‐fat levels are other exam-
ples of cultural change.
New Technology
and Packaging
Advancements in technology bring many opportunities for
companies that simply pay attention. More and more com-
panies are engaging in an open innovation process that
High‐Pressure Processing (HPP):
A Technology Breakthrough
Until recent years, many refrigerated products, especially pro-
tein‐based items such as deli meats and fresh shellfish, had
short shelf lives due to growth of pathogenic and spoilage
bacteria. These product categories are typically expensive to
manage and have unique cost implications due to waste and
shrink. High‐pressure processing is a safe bacteria‐control
process that doubles or triples the shelf life of many of these
products. HPP involves packaged food products being sub-
jected to many atmospheres of water pressure, which disrupts
the cell structure of any living organisms present and thus
stops the growth of spoilage organisms. Not all foods work with
HPP; the food matrix must have an even distribution of free
water and be packaged appropriately.
This technology is widely used for avocado products.
Avocados are problematic because of unpredictable levels
of ripeness. Additionally, they must be pitted, skinned, and
used quickly, as their flesh rapidly oxidizes and turns brown.
HPP technology allows avocado manufacturers to pro-
duce pulp (for guacamole), chunks, and even individually
pouched, peeled, and pitted avocado halves for fresh distri-
bution with a long (refrigerated) shelf life. Incorporation of
ascorbic acid greatly reduces oxidation and thus browning.
These avocado products have been well received in spite of
their higher cost, which is often offset by reduction in waste
and labor in the kitchen versus handling fresh avocados.
The Pizza Evolution:
A Real‐Life Experience
Some years ago a pizza manufacturer launched a new item.
The original product was excellent and sold well. However,
over a 14‐month period post‐launch, the following modifica-
tions were made:
• A different combination of yeast strains was used to
decrease the amount of proof time for the dough (22 per-
cent reduction).
• A better dough conditioner was found to improve
extensibility.
• A gum was added to increase shelf life, and a secret flavor
was added to make the taste more craveable.
• A “food glue” was developed to make an herb dusting
adhere more firmly, as it tended to fall off during shipping,
and an atomizing nozzle had to be engineered to apply
it—no small task, as the glue wanted to stick to the nozzle
jets, too!
The result of these optimizations was a measurably supe-
rior product. This optimization process has the added benefit
of leveraging the moving target theory, which states that it
becomes much more difficult for competitors to copy a prod-
uct when it incrementally but continuously improves.
c19.indd 412 1/14/2016 7:57:39 AM
CHAPTER 19 • Commercializing the Culinary Gold Standard • 413
continuously seeks breakthrough competitive advantages
wherever they may be. Many companies rely only on internal
research and development, which is self‐limiting in scope;
open innovation seeks ideas and technologies outside of
internal sources. Research universities, venture partnerships,
government R&D, and think tanks are examples of external
resources; others can be as close as your ingredient, equip-
ment, or packaging vendors.
Ten years ago, shelf‐stable meats and seafood only came
in cans. Now they can be purchased in pouches and even
plastic cups; these products are often better‐tasting as well as
more convenient and eco‐friendly. The military and the
National Aeronautics and Space Administration (NASA) are
often sources of packaging innovation due to their inherent
need for high‐quality shelf‐stable food products whose shelf
life is measured in months or even years, often under adverse
conditions.
Life Cycle: Process
Improvements
In life, through repetition, observant people find more efficient
ways of doing things. A good food technologist, engineer, or
plant operations manager can develop modifications to manu-
facturing processes or equipment to improve finished prod-
ucts or make them faster and more efficient to produce. The
level of skill at achieving this is an important competency often
found in great companies. Rarely does a single big “AHA!”
moment lead to a breakthrough improvement; rather, a series
of small, painstaking, incremental improvements can lead to a
whole greater than the sum of the parts.
A well‐managed product optimization process, done for
the right reasons, is a distinct competitive advantage. There
are very few cash‐cow food products that remain perennial
category leaders year after year. Most products and prod-
uct lines need skilled, holistic management to keep them
relevant to consumer needs in an increasingly competitive
market.
Conclusion
No product development and commercialization process
is successful all of the time. However, a sound, disciplined
process can greatly increase the success rate and generate
more winners than losers. High levels of risk tolerance and
acceptance of failure as part of the learning experience are
key components of innovative cultures.
Some corporate cultures don’t encourage risk‐taking; in
fact, overly high expectations of success decrease creativity,
as teams limit their efforts to the certainly achievable. Those
cultures are generally devoid of innovative, breakthrough
products that produce handsome margins; instead, they are
relegated to copying the winning products others develop.
This yields commoditized margins and the expensive propo-
sition of buying market share—not the pathway to break-
through innovation.
c19.indd 413 1/14/2016 7:57:39 AM
414 • CULINOLOGY®
A
Acetic acid, 201–203
Acidification, 260, 264, 279, 280–81, 283
Acidulants, 236–38
Actomyosin complex, 89, 99
Aerobic microorganisms, 196, 198, 202, 203
Affective/preference tests, 353
Agglomerated, 154
Air‐blast freezing, 376–77, 387
Air classification, 159
Albumin, 180
Alcohol production, 203–208
Alkaline phosphates, 59, 73
Alpha, beta‐prime, and beta crystals, 172
Alpha‐linolenic acid (ALA), 173–74
Amidated LM pectins, 162
Amine groups, 59
Amino acids
branched chain, 327
limiting, 57
in proteins, 57–60, 74
Amphiphilic compounds/proteins, 171
Anabolism, catabolism, metabolism, 197
Anaerobic bacteria/microorganisms, 68, 75
Anaerobic fermentation, 62
Anaerobic glycolysis, 86
Anaerobic packaging, 91
Antimicrobial preservatives, 229–30
Antioxidants, 220–22
natural, 74, 295
polyphenolic, 294
synthetic, 74–75, 295
Ascorbic acid, 221, 230, 236
Aseptic processing/packaging, 374–76
Aspergillus niger/oryzae, 237
Aspetic processing/packaging, 287
Atherosclerosis, 174
Attitudinal approaches, 354, 355
Attribute difference tests, 357
Autolyzed yeast extract (AYE), 74
Autoxidation, 91
B
Bacillus cereus/licheniformis/pumilis/
subtilis, 229
Bacteria, 288, 290–91
Bacterial growth curve, 200, 201
Bacterial pathogens, 259–60
Baking, 46–47
Baking powder/soda, 228, 238
Barbecue, 49–50
Barley, 121
Beef grading, 64–66
Behavioral approaches, 354
Beta‐carotene, 223, 224
Beta‐glucans, 332, 333, 348
Bioaccumulation, 91, 93, 98, 103
Bioactive compounds, 347–49
Biodegradable packaging, 319
Bio gum, 153–55
Biomolecules, 132
Bivalves, 85, 97–99
Blanching, 52–53
Bloom (on chocolate), 172
Bloom strength, 163
Body mass index (BMI), 132–33
Boiling, 52–53
Bone char, 107
Botulinum toxin, 250, 254, 260,
262, 264
Bovine spongiform encephalopathy (BSE),
68–69
BPA (bisphenol A), 317
Braising, 54
Brand management, 14
Bread, 211–12, 281
Brix, 206, 385
Broiling, 49
Bromelain, 391
Browning reactions, 291–94, 296, 298, 299
Buckwheat, 122
Buffers/buffering capacity, 59
Bulking agents, 143–44, 222
Bulk phase, 34
Butter, 199, 208
C
Calories, 322, 323
Calorimeters, 322
Campylobacter, 69–70
Canning, 372–74
Caramel color, 223–24, 226
Carbohydrates, 19–24, 128–65
complex, 132, 133
Carbon dioxide gas, 196, 200, 205,
207, 209, 211
Carbon monoxide gas, 296, 302
Carbonyl group, 40
Carboxyl groups, 59
Carboxymethylcellulose (CMC), 158,
160–61
Carboxymyoglobin, 88
Carnivores, 106
Carrageenan, 75
Casein, 186, 189, 190–91
Catalyze, 183
Cations and anions, 155
Cavity ice, 313
CDC (Centers for Disease Control), 69, 70
Celiac disease, 118, 121, 123, 124, 344
Cellulose, 332, 347
Cephalopods, 85, 99–100
Cereal products, 281–82
Characterizing flavor, 217
Cheeses, 189, 190–92
Chelating agents, 100, 220–21, 228,
236, 237
Cholesterol, 166–67, 172, 173, 174, 178,
328–29
Chopped and formed, 81, 82, 83
Churning, 167, 171
Chymosin/rennin/rennet, 190–91
Cis fatty acid, 170
Coacervation, 296
Coagulation, protein, 59, 60, 73
Coalesce, 154
Cochineal/carmine, 224
Coenzymes/cofactors, 183
Coextrusions, 307, 316
Coffee fermentation, 214
Cold fill, 284
Cold shortening, 63
Cold spot, 373, 374
Collagen, 50, 54, 55
Colloids/colloidal dispersion, 145, 158,
161, 162, 163–64
Colorimetric confirmation test, 371
Colors, 222–25
Commensalism, 202
Commercialization, 17
Competitive set/frame, 399–400
Index
Index_b01.indd 414 1/18/2016 8:17:33 PM
INDEX • 415
Complementation, protein, 57
Complete proteins, 57, 58, 61
Concentrated/condensed foods, 381–83
Conduction/conductive heating, 43–45
Conjoint analysis, 360, 361
Conjugated linoleic acid (CLA), 330, 348
Connective tissue, 58, 60, 63, 65, 67–68,
71–72
Consumer acceptance tests, 356–57
Consumer insights, 17
Controlled atmosphere (CA), 275, 281
Convection/convective heating, 44–45
Converted rice, 119
Cook‐chill, 387–88
Cooked yield, 391, 392
Cooking techniques, 46–56
dry‐heat, 46–50
hot‐oil, 50–52
moist‐heat, 52–55
thickeners, 55
Corn, 120
Corn syrups, 128, 134, 143–44
Critical control point (CCP), 63
Critical relative humidity, 32
Cross‐linked starches, 141
Crude oil, 171
Crustaceans, 85, 100–102
Cryogenic freezing, 377
Cryoprotectants, 92, 93
Cryovac, 67
Culinary gold standard, 352, 396
Culinology careers, 16–17
Curds, 197, 208, 209–10
Cyclone/vortex separators, 379
D
Dairy foods, 180, 186–94
fermentation of, 208–11
spoilage, 282–84
Death phase, 200
Deep frying, 51–52
Dehydration, 378–81
Deliquescence, 24, 32–33, 36
Denaturation, protein, 59, 60
Depolymerization, 143
Descriptive analysis, 354, 355, 358
Dextrins/dextrinization of starches,
119, 120
Dextrose, 74, 75
cultured, 230
Dextrose equivalent (DE), 74
Diabetes, 133, 134, 144
Diacetyl, 208
Diatomaceous earth, 171
Dietary Guidelines for Americans,
323, 325, 326, 334, 342
Diglycerides, 176, 225–27
Dipole moment, 20, 29
Dipotassium phosphate, 228
Direct acidification, 238
Direct heat transfer, 44
Disaccharides, 22–23, 24
Discontinuous/dispersed/internal
phase, 175
Discrimination methods, 354, 357–58
Disgorgement, 207
Disodium EDTA, 107
Dissimilation, 198–99
Distillation, 171
Dressing percentage, 79
Drip loss, 87, 89–91
Drum‐drying, 142
Dry weight basis (DWB), 152
D‐value (decimal reduction time), 371, 372
Dyes/lakes, 223, 224–25
E
E. coli, 70–71
Eggs, 180–86, 192–93
spoilage, 282
Emulsified dressings, 284
Emulsifiers, 25, 30–32
additives, 225–27
dairy, 190
eggs, 185
Emulsifying capacity, 92
Emulsions, 30–32
meat, 80, 81, 82
Encapsulation, 296, 297
Endospores/sporulation, 260
Energy density, 323–24, 342
En papillote, 53–54
Enzymes, 57, 58, 60, 63, 68, 74, 75
meat tenderizing, 68
Essential amino acids, 57, 61
Essential fatty acids (EFAs), 173–74
Ester bonds/linkages, 21, 24, 27
Ester functional groups, 316
Ethanol, 196, 201–202, 203, 208, 218
Ethylene vinyl alcohol (EVOH), 307, 316
Evisceration, 63, 78–79
Expert panels, 354, 358
Exponential growth, 200
Exsanguination, 63
Extended shelf life (ESL), 307, 310
Extended shelf life refrigerated (ESLR), 312
Extrusion, 386–87
F
Facultative anaerobes, 198, 203
Falling rate, 378
Fats/lipids, 19, 21, 22, 24–27, 43, 44, 166–79
functions of, 175–79
oxidation, 377
processing, 171–74
sources of, 166–69
structure, 169–71
FD&C, 225
Fermentable substrates, 196, 203
Fermentation, 59, 71, 74, 75, 196–219
anaerobic, 62
stuck, 201
Fermentative lactic acid bacteria, 75
Fermenters, 203, 205–206, 211
Fiberboard, corrugated, 306, 308,
315, 319
Fibrous proteins, 28, 39
Finished product specification, 408
Flashing off, 299
Flash point, 178
Flat sour organisms, 276
Flavonoids/catechins, 348, 349
Flavor pyramid, 232–33
Flavors, 230–38
artificial and natural, 217, 218, 231–35
compounded, 232–33
enhancers, 235–36
potentiators, 217
Fluidized bed freezing, 377, 378–79
Foams, 185–86, 191
Food additives, 220–44
Food and Drug Administration (FDA), 9
Food‐at‐home, 5–6
Food‐away‐from‐home, 6–8
Foodborne illnesses
HACCP and, 252–62
major pathogen hazards, 248–52
outbreaks of, 246–47
seafood, 93–95
Food industry, 2–3
segments in, 4–8
Food intolerances/sensitivities, 342–44
Food processing, 365–95
Food safety and spoilage, 245–85
HACCP and, 252–70
packaging and, 310–15
processes for, 264–65, 270
process temperatures, 262–64
Food safety objective (FSO), 254, 264–65
Food science, 19–45
heat‐driven reactions, 38–41
heat processes, 41–45
molecular mobility, 37–38
nature of matter, 19–21
solids, 21–29
water, 29–37
Free non‐protein nitrogen (NPN), 277
Free radicals, 178–79, 221, 227
Freeze drying/lyophilization, 379,
380–81
Freezer burn, 90–91
Freezing, 376–78
Freshness/best‐by dating, 286, 288
Fresh pack, 200
Fructo‐oligosaccharides, 162
Fruits
climacteric/nonclimacteric, 301–302
preserves, 383–86
Fruits, spoilage, 279–81
Functional foods, 182
Functional proteins, 87, 91, 92, 99
Fungi, 271, 278, 280, 281, 282
Fusion, latent heat of, 42
Index_b01.indd 415 1/18/2016 8:17:33 PM
G
Galactomannan family, 146
Gas chromatography/mass
spectrometry, 232
Gas flushing, 67
Gastropods, 85, 95–96
Gelatin, 163–64
Gelatinization, 38, 129, 139, 140, 141, 142
Gelatins, 55
Gelation, 80–81
Gels, 28, 38, 39
Generally recognized as safe (GRAS), 8
Glacial acetic acid, 201, 202
Globular proteins, 28, 39
Glucomannan, 159, 160
Glutamates/glutamic acid, 114, 116
Gluten, glutenin, gliadin, 58
Gluten intolerance, 344
Glycemic index, 134, 136, 151, 160
Glycerol backbone, 24–25, 27
Glycogen, 86, 87, 98
Glycolysis, 132
Glycoproteins, 180
Gonads, 87, 94
Good Manufacturing Practices (GMP),
252, 270, 277
Grains, 116–25
Gram‐positive/‐negative organisms, 199,
200, 202
Gray (Gy)/kilogray, 389
Grilling, 48–49
Growth, sources of, 1–2
Gums, 82, 144–63
H
Halophiles, 200, 213
Hazard Analysis and Critical Control
Points (HACCP), 63, 252–70
Heat labile, 260
Heat transfer, 19, 22, 30, 41–45
Hedonic scale, 356
Hemaglutenins, 214
Hemoglobin, 58–59, 66
Hemolytic uremic syndrome (HUS), 70
Herbivores, 106
Hermetically sealed, 372, 373, 388
Hexoses, 22
High‐density lipoproteins (HDL), 167, 174
High‐fructose corn syrup (HFCS), 128, 134,
135–36, 142, 143–44
High‐methoxyl pectin, 161–62
High‐pressure processing (HPP), 287,
389–90, 412
Histamine, 94
Hold phase, 139
Homeostasis, 62
Homogenization, 184, 185, 188, 190
Homogenizers, 375
Homo‐/hetero‐fermentative, 200, 201
Honey, 284
Horizontal marketing, 402
Hot‐fill‐and‐hold processing, 320
HTST (high temperature, short time),
147, 162
Humectants, 222
Hurdle technology, 288, 290, 291
Hydrocolloids, 162, 163–64
Hydrogenation, 168, 170, 172–73, 178, 179
Hydrolysis/hydrolization, 23, 24–27,
33, 34, 40
Hydrolyzed vegetable protein (HVP), 74
Hydroperoxide, 178–79
Hydrophilic, 27, 31–32, 145, 152, 154, 156, 161
Hydrophobic, 27, 28, 31–32, 88, 91, 98
Hygroscopic, 133–34, 135, 137, 144
Hypertension, 342
I
Immersion freezing, 377
Immiscible, 30
Induction heating, 45
Inductive heating, 390
Initiation stage, 178
Injection marination, 391
Inorganic acids, 236, 238
Inorganic packaging materials, 320
Inosinate and guanylate (I + G), 74
In situ preservation, 290
Intelligent packaging, 307
Interaction energies, 21
Interfacial tension, 22, 31, 32
Intrinsic/extrinsic factors, 271–75
Inulin, 162
Invertebrates, 85, 98
Invert sugar, 136
Ionic strength, 59
IQF (individually quick frozen), 366, 377, 379
Irradiation, 388–89
Isoelectric point, 59
Isomers/isomerization, 22
J
Jellying point, 385
Junction zones, 38, 39
Just‐about‐right scale, 356
K
Ketones, 21, 22
Kjeldahl digestion, 87
Kosher/halal, 337–38
L
Labeling, shelf‐life, 288
Lactic acid, 197, 199–201, 200, 201, 208,
209–10, 213, 218
Lactobacillus, 199–200, 209, 211
Lactose intolerance, 342–43
Lag phase, 200
Laminates, 307, 315, 317
Lard, 106, 172
Launching, 396–97, 409–11
Leavening, 203, 205, 211–12
Leavening agents, 228
Lecithin, 88
Legumes, 107–10, 282
Leuconostoc cremoris/mesenteroides, 208,
209, 213
Line extensions, 11–12
Lipids. See Fats/lipids
Lipolysis, 298
Lipophilic emulsifiers, 32
Lipoproteins, 174
Log cycle/5‐log reduction, 371
Log phase, 200
Log reduction, 48
Long‐chain fatty acids (LCFA), 168, 172
Low‐acid food, 108
Low‐density lipoproteins (LDL), 121
Low‐methoxyl pectin, 162
M
Mad cow. See Bovine spongiform
encephalopathy (BSE)
Malting, 205
Maltodextrins, 137, 143–44
Marbling, 62, 64–66
Marination, 60, 66, 83, 391–94
Market benchmarking, 399, 402, 403
Market testing, 409–10
Mashing, 135
Mass transfer, 378
Material Safety Data Sheets (MSDS), 235
Meat, 61–76
cookery, 71–73
cured, 67, 74–75
fermentation, 212–13
grading, 64–66
industry concerns, 68–71
pigments, 60, 66–67, 74, 75
processing, 391–94
processing ingredients, 73–75
production, 62–68
spoilage, 275–77
Mechanically separated chicken, 81
Melting point, 168, 169, 170, 171, 172
Metabolic syndrome, 134, 136
Metmyoglobin‐reducing activity (MRA), 66
Microaerophiles, 248, 273
Microbial growth temperatures, 260, 273
Micronutrients, 321, 334–37
Microparticulated proteins (MPP), 222–23
Microwave heating, 45
Microwave susceptor, 317
Minimally processed foods, 231, 370
Minimally processed refrigerated
(MPR) foods, 238
Miso, tempeh, soy sauce, 110, 114, 203,
215–17
Mitosis/meiosis, 327
Modified atmosphere packaging (MAP), 67
Moisture sorption isotherms, 37
Moisture‐to‐protein ratio (MPR), 212, 213
Molasses, 135
Molecular mobility (Mm), 29, 33, 37–38,
41–42
416 • CULINOLOGY®
Index_b01.indd 416 1/18/2016 8:17:33 PM
Mollusks, 87, 95–97
Monoglycerides, 176
Monosaccharides, 22–24, 40–41
Monosodium glutamate (MSG), 74
Monounsaturated fats, 325, 328–29, 348
Mucilage, 214
Multiple paired preferences, 356
Muscle fibers, 61, 63, 65, 71–72
Musculoskeletal/cytoskeletal proteins, 327
Must, wine, 206
Mycoprotein, 126
Myocommata, 86, 87, 99, 102
Myofibrillar proteins/myofibrils,
59, 60–61
Myotomes, 93, 96, 97
MyPlate, 325–26, 342
N
Natamycin, 229–30
Natural Certification, 235
Natural colors, 222–24
Natural convection, 44
Negative heat of solution, 137
Net protein utilization (NPU), 57, 58
Nisin, 230
Nitrites, 230, 231
Nitrosamines, 74–75
Nitrosyl hemochrome, 66
Non‐ionizing radiation, 45
Non‐reducing sugars, 74
Nutraceuticals, 4, 151, 160
Nutrition, 321–51
processing and, 367–69
Nutrition facts panel (NFP), 322
Nutrition Labeling and Education Act
(NLEA), 235
Nutritive sweeteners, 238
Nuts and seeds, 125–26, 282
Nylon (polyamide), 306, 317, 318
O
Oats, 121–22
Offal, 61
Ohmic heating, 390
Olfaction, 230
Oligosaccharides, 109, 114
Omega‐3 fatty acids, 88, 102, 329–30
Omega‐6 fatty acids, 88, 329–30
Omnivores, 106
Open shelf‐life dating, 288
Organic (carbon‐containing), 299
Organic acids, 229–30, 238
Organoleptic analysis, 352
Osmosis, 200
Osmotic preserves, 383–86
Overall difference tests, 357
Oxidation, 88, 91, 93
Oxidation catalyst, 88
Oxidative rancidity, 26–27
Oxidized, 262, 271, 273, 275, 276
Oxygen radical absorbance capacity
(ORAC), 349
Oxygen scavengers, 307, 315, 320
Oxymyoglobin, 66
Ozone/ozonation, 259, 262, 275
P
Package material converting, 306
Packaging, 13, 17, 305–20
Paired preferences, 356
Pan‐drying, 51–52
Paperboard, 306, 307, 308, 315, 319
Par‐boiling, 53
Parching, 119–20
Pasteur, Louis, 197
Pasteurization, 200, 202, 208–10, 216
cold, 287
flash, 371
Pasting temperature, 139
Pearling, 118, 119, 120, 121, 123
Pectin, 109
Pediococcus acidilactici/cerevisiae, 200, 212
Penicillium spp., 275, 276, 279, 281, 282, 283
Peptides, 57, 59
PER (protein efficiency ratio), 87
Permeation, 318
Peroxidase, 370
Peroxide free radicals, 178–79
PH, 62, 83
equilibrium, 280
Phase transitions, 30, 32, 42, 43
Phenolics, 349
Phenylketonuria (PKU), 238
Phosphates, 81, 82, 83, 227–28
Phospholipids, 32
Phosphoric acid, 236, 237, 238
Phosphorylated molecules, 74
Photo‐oxidation, 286, 295, 301
Phytonutrients, 324, 349
Plastic fats, 29
Plasticizers, 33, 34, 38
Plasticizing, 172
Plate freezing, 377
Plate heat exchangers, 376
Plating medium, 222
Poaching, 53
Polar/non‐polar molecules, 224, 225,
227, 232
Polyester, 307, 308, 313, 316–18
Polyethylene (PE), 306, 308, 314, 316–17, 318
Polyethylene terephthalate (PET),
316, 317, 318
Polymerization, 178
Polymorphism, 29
Polyolefins, 316
Polyols/sugar alcohols, 137
Polypropylene (PP), 306, 308, 314, 316, 318
Polysaccharides, 22–24, 39
Polystyrene (PS), 311, 317, 318, 319
Polyunsaturated fatty acids, 88, 91, 100,
102, 103
Polyvinyl chloride, 311, 317, 318
Potassium chloride, 82–83
Potentially hazardous foods (PHF), 387–88
Potentiators, 217
Poultry, 77–84
processing, 391–94
spoilage, 278
Prebiotics/probiotics, 109, 346–49
Preference tests, 355–56
Pressure‐cooking, 54
Primal cuts, 64, 67
Primary structure of proteins, 58
Prion proteins, 68
Product development, 1–18, 397–409
classification of, 10–14
food industry segments and, 4–8
government influences on, 8–10
sales/marketing influences on, 14–15
societal influences on, 10
strategies for global, 15–17
teams for, 17–18
technology in, 4
Product life cycles, 3–4
Product optimization, 411–13
Project managers, 17
Pro‐oxidants, 294, 295
Propagation stage, 178–79
Protein Digestibility Corrected Amino
Acid Score (PDCAAS), 58, 327–28
Proteins, 19–22, 27–28, 39–41
chemical structure, 57–61
complete/incomplete, 57, 58, 61
meat, 57–76
in nutrition, 327–28
poultry, 77–84
seafood, 85–105
vegetable, 106–27
Proteolysis, 203
Protons, neutrons, electrons, 59
Proximate composition, 103
PSE, 62–63
Pseudo emulsion, 152, 154, 158
Pseudoplastic, 153–54
Pulsed‐light processing (PL), 390–91
Pulses, 107, 109
Purchase intent, 354, 355, 356
Pureeing, 55
Purgatives, 94
Purges, 156, 158
Purging, 201
Pyrolysis, 133
Pyruvate, 198–99
Q
Qualitative consumer research, 353,
354–55
Qualitative method, 201
Quantitative consumer research, 353, 355
Quaternary protein structures, 27–28, 38
Quorn, 126
R
Radiant energy/radiation, 287, 295, 297,
300, 388–89
Radio‐frequency (RF) drying, 390
INDEX • 417
Index_b01.indd 417 1/18/2016 8:17:33 PM
Radura, 389
Rancidity, 22, 25, 26, 27
Reaction/processed flavors, 232
Ready‐to‐eat (RTE), 71, 74
Recrystallization, 333
Recycled packaging, 315, 317–18, 320
Redox potential, 271
Reduced‐oxygen packaging (ROP),
387–88
Reducing sugars, 22, 24, 37, 40–41
Reductants, 75
Reductions, 55
Refining, 171–72, 173, 175
Refractometer, 385
Relative humidity (RH), 32, 35, 36, 37
Rendering, 171
Respiration, 289, 290, 302
Respiratory anaerobiosis, 310, 311, 317
Restaurant foods, 14
Resting time, 48
Restructured meat, 73, 75
Retail grocery, 5–8, 14–15
Retort sterilization, 91, 97, 100
Retrogradation of starches, 29, 38, 131, 133,
139–41
Reverse osmosis, 382–83
Rheological property, 80
Rhizopus oligosporus/oryzae, 113
Rice, 119–20
Rigor mortis, 63
Roasting, 47–48
Rope spoilage, 281
Roux, 55
Ruminants, 62, 69
Rye, 117, 118, 122
S
Saccharomyces cerevisiae/bayanus, 202,
206, 208, 211, 218
Salmonella, 70
Salometer, 200–201
Salt bridges, 38
Salt‐soluble proteins, 60, 73
Sarcomeres, 93
Sarcoplasmic proteins, 59–60
Sautéing, 50–51
Scale‐up testing, 409
Scraped‐surface heat exchangers,
376, 377
Screw and crown closures, 314
Seafood, 85–105
classifications, 85
fermentation, 213
finfish, 83–95
foodborne illnesses, 93–95
freshness in, 86–89
nutritional value of, 102–103
preserving, 89–93
shellfish, 95–102
species substitutions, 93
spoilage, 277–78
Seitan, 124–25
Senescence, 301–302
Sensible heat, 41–42
Sensory descriptive analysis, 358
Sensory discrimination tests, 357
Sensory evaluation, 352–64
Sensory‐liking analysis, 357, 361–62
Sequestrants/sequestering agents,
220–21, 294
Serotypes, 70
Serving size, 322–23, 336
Setting point, 155
Shear, 139, 140, 141, 143, 146, 147
Shelf‐life extension, 286–304
analyzing/predicting, 302–304
extrinsic/intrinsic factors in, 301–302
food changes during, 288–301
Shelf‐stable foods, 262, 264, 265, 276, 283
Shellfish poisoning, 94–95, 96
Shiga toxin (Stx), 70
Shortening, 169, 170, 175, 176
Simmering, 52–53
Single‐cell proteins, 126
Skeletal muscle, 61, 66
Smoke point, 52, 178
Sodium alginate, 149, 157–58
Sodium ascorbate, 75
Sodium diacetate, 74
Sodium erythorbate, 75
Sodium lactate, 74
Sodium lauryl sulfate, 184, 185, 186
Sodium nitrate/nitrite, 74
Sodium reduction, 73–74, 82–83
Solanine, 129
Solid Fat Content (SFC), 170–71
Solid Fat Index (SFI), 170–71
Solute, 134, 145
Solvent, 134, 145
Solvent extraction, 171
Sorghum, 118, 122–23
Source reduction, 319
Sourdough, 196, 211
Sous vide, 54–55, 387–88
Soybeans/soy foods, 110–16
fermentation of, 214–17
Soy flour, 82
Soy proteins, 82
Specifications, 408–409
Specific gravity, 205
Specific heat, 43
Spices and herbs, 281
Spray‐chilling, 296
Spray‐drying, 142, 152, 164
Stack heat, 140
Stage‐Gate process, 397
Standard of Identity, 91, 98
Starches, 22–24
chemically modified, 141–42
converted, 143–44
gelatinization of, 38
hydrolysates, 223
native, 139, 140–41
resistant, 333–34
selecting, 142–43
stabilized, 141–42, 163
thickeners, 55
Starter distillate, 167
Stationary phase, 200
Steaming, 53
Steam injection, 375, 382
Steric hindrance, 31
Sterilization, 260, 265, 277, 279, 282–83
Stewing, 54
Stir‐frying, 51
Streptococcus lactis/natalensis, 230
Stromal proteins, 60
Structural muscles, 61
Sublimation, 90, 93
latent heat of, 42
Subprimals, 67
Succinic acid, 238
Sugars, 133–37, 284
Suppliers, 8
Sweeteners, 238–39
nutrients in, 331–32, 346
Swelling, hot/cold, 81, 82
Synergism, 146, 150, 151, 153–54
Syrups, 284
T
Textured vegetable protein (TVP), 115
Thermal radiation, 45
Thermo‐reversible gels, 19–20, 24, 39
Thickeners, 55
Tofu, 111–14
Trans fats, 167, 172–73, 174, 178
Trichina spiralis, 69
Triticale, 122
Tubular exchangers, high‐velocity/
pressure, 376
U
United States Department of Agriculture
(USDA), 9–10
meat inspections, 63–64
V
Vanilla fermentation, 217
Vaporization, latent heat of, 42
Vegetables, 106–27
fermentation, 213–14
spoilage, 278–79
Vegetarian diets, 106–107
Vinegars, 201–203
Vitamins, fat‐soluble, 299–301
W
Wheat, 120–21
Y
Yeast, 202, 203–205
418 • CULINOLOGY®
Index_b01.indd 418 1/18/2016 8:17:33 PM
WILEY END USER LICENSE AGREEMENT
Go to www.wiley.com/go/eula to access Wiley’s ebook EULA.
http://www.wiley.com/go/eula
Cover������������
Title Page�����������������
Copyright
Contents���������������
Foreword to Culinology®: The Intersection of Culinary Art and Food Science���������������������������������������������������������������������������������
Preface: So What the Heck Is Culinology®, Anyway?
Acknowledgments����������������������
Chapter 1: The Business of New Product Development and the Role of the Culinology® Professional������������������������������������������������������������������������������������������������������
Introduction: Why Is New Product Development Important?
Sources of Growth
The Food Industry
Food Development Today
Product Life Cycles
Technological Influences on New Product Development
Food Industry Segments
Food-at-Home
Food-Away-From-Home
The Supply Side of the Business
Food Ingredient Suppliers
All Other
Governmental Influences on New Product Development
The Food and Drug Administration and the U.S. Department of Agriculture
FDA
USDA
Societal Influences on New Product Development
Classification and Characterization of New Food Products
Line Extensions
Repositioned Existing Products
New Forms of Existing Products
Reformulation of Existing Products
New Packaging or Brand Labeling of Existing Products
New-to-the-Company Product Lines
New-to-the-World Creative Products
Sales and Marketing Influences on New Product Development
Restaurant Foods
Retail Grocery
Strategies for Global Product Development
The Role of the Culinology® Professional
Why Consider a Career as a Culinology® Professional?
Creating and Managing Product Development Teams
Project Manager
Product Developer
Consumer Insights
Packaging
Commercialization
Regulatory
Conclusion
Chapter 2: The Principles of Food Science������������������������������������������������
Introduction
The Nature of Matter
Types of Solids
Carbohydrates
Lipids
Proteins
Physical Structures (Amorphous and Crystalline) of Solids
Water
Water-Solid Interactions
The State of Water When Solids Are Present
Water Activity
Moisture Sorption Isotherms
Molecular Mobility
Heat-Driven Chemical Reactions in Foods
Gelatinization of Starches
Denaturation of Proteins
Gelation (Gel Formation)
Non-Enzymatic Browning Reactions
Heat as Individual Processes
Phase Transitions
Thermal Properties
Heat Transfer
Conduction
Convection
Thermal Radiation
Microwave Heating
Induction Heating
Chapter 3: Review of Cooking Techniques����������������������������������������������
Understanding Cooking Techniques from a Chef's Viewpoint
Dry-Heat Cooking Methods
Baking
Roasting
Grilling
Broiling
Barbecue
Hot-Oil Cooking Methods
Sautéing
Stir-Frying
Pan-Frying and Deep Frying
Moist-Heat Cooking Methods
Boiling, Blanching, and Simmering
Poaching (Shallow and Deep)
Par-boiling and Steaming
En Papillote
Pressure-Cooking
Braising and Stewing
Immersion Cooking (Sous Vide)
Thickeners
Roux
Reductions
Starches
Gelatin
Pureeing
Conclusion
Chapter 4: Protein-Based Foods: Introduction and Red Meats�����������������������������������������������������������������
Introduction to Protein Chemistry
Nutritional Properties of Proteins
Protein Structure
Functional Properties of Proteins
Muscle Structure
Meat
Meat Production
Common Concerns in the Meat Industry
Bovine Spongiform Encephalopathy
Parasites: Trichina spiralis
Bacterial Concerns
Meat Cookery
Changes During Cooking
Meat Processing Ingredients
Conclusion
Chapter 5: Protein-Based Foods: Poultry����������������������������������������������
Introduction
Pre-Harvest Handling
Stunning
Killing and Bleeding
Scalding and Feather Removal
Evisceration
Chilling and Carcass Aging
Dressing Percentage
Poultry Types
Basic Cuts of Poultry
Poultry Meat Functionality
Further-Processed or Value-Added Poultry
Portioning
Ingredient Functionality and Flavor Enhancement
Forming Strategies
Conclusion
Chapter 6: Protein-Based Foods: Seafood����������������������������������������������
Introduction
Seafood Classifications
Market Forms of Finfish
Muscle Structure of Finfish
Identifying Freshness in Seafood
Rigor and pH
Fresh Finfish Protein Quality
Fat Content
Dark and Light Flesh
Adenosine Triphosphatase (ATPase) Test for Freshness
Preserving Seafood
Freezing
Canned Fish
Restructured Fish
Salting: Caviar and Preserved Roe
Treating Seafood with Phosphate
Economic Fraud and Species Substitution
Fish- and Shellfish-Derived Foodborne Illnesses
Shellfish
Shellfish Certification
Mollusks
Bivalves: Clams
Bivalves: Mussels
Bivalves: Oysters
Bivalves: Scallops
Cephalopods: Squid, Octopus, and Cuttlefish
Crustaceans
Other Seafood
Nutritional Value of Seafood
Protein Quantity and Quality
Fat Content
Mineral Content
Conclusion
Acknowledgments
Chapter 7: Protein-Based Foods: Vegetable Sources of Protein and Protein Complementation�����������������������������������������������������������������������������������������������
Introduction: The Culture of Vegetarian Diets
Vegetable Sources of Protein
Legumes
Soybeans and Soy Foods
Grains
Conclusion
Chapter 8: Carbohydrate-Based Foods������������������������������������������
What Are Carbohydrates?
Carbohydrates as an Ingredient: The Chef's View
Grains, Legumes, and Starchy Vegetables
Flours
Refined Starches
Carbohydrates in Nutrition
Carbohydrates as Ingredients
Sugars
Monosaccharides
Disaccharides
Polyols
High-Intensity Sweeteners
Native Starches
Chemically Modified Starches
Starch Selection
Converted Starch Products: Maltodextrins and Corn Syrups
Gums
How to Use Gums
Types of Gums
Seed Gums
Tree Exudates
Bio‐Gums
Marine Gums
Plant Gums of Terrestrial Origin
Less Used Gums
Stabilizer Blends
Gelatin
Conclusion
Chapter 9: Lipid-Based Foods�����������������������������������
Introduction
Historical Perspective
Fat Sources
Vegetable Oils
Animal Fats
Dairy Fat (Butter)
Lipid Structure
Processing of Fats and Oils
Nutrition and Essential Fatty Acids (EFAs)
Cholesterol and Saturated Fat
Functions of Fats and Oils in Food and Cooking
Lubricity
Intrinsic Flavor
Carrier/Promoter of Flavors and Colors
Mouthfeel
Emulsions
Heat Transfer
Baking
Frying
Rancidity
Conclusion
Chapter 10: Egg- and Milk-Based Foods��������������������������������������������
Working with Dairy and Egg-Based Products
Eggs
Composition of Eggs and Egg Components
Nutrition
The Value of Egg Products in Food Product Development
Coagulation, Thickening, Coating, and Binding
Emulsification
Foaming
Controlling Crystallization
Milk
Composition of Milk and Milk-Based Products
Nutrition
The Value of Dairy Products in Food Product Development
Milkfat Function
Water-Holding Capacity
Color and Browning
Emulsification
Gelation and Thickening
Whipping and Foaming
Cheese Applications
Applications
Culinology® Case Study in Product Development Using Egg Products
Culinology® Case Studies in Product Development Using Milk Products
Conclusion
Chapter 11: Fermentation�������������������������������
Introduction
History of Fermentation
Preservation of Perishable Products by Fermentation
Process of Fermentation
Lactic Acid Bacteria
Acetic Acid Bacteria and the Production of Vinegar
Alkaline Fermenters
Molds
Yeast
Alcohol Production
Beer-Making Ingredients
The Science of Beer-Making
Making Wine
Champagne and Other Sparkling Wines
Wine Fermentation
Dairy Fermentations
Bread
Meat Fermentation
Fish Fermentation
Vegetable Fermentation
Coffee Fermentation
Soy Fermentation
Vanilla Fermentation
Biotechnology Applications in Fermentation
Vitamin Production
Industrial Chemicals
Conclusion
Chapter 12: Food Additives���������������������������������
Introduction
Food Additives for Function
Sequestrants and Antioxidants
Bulking Agents
Colors
Emulsifiers
Phosphates
Chemical Leavening Agents
Antimicrobial Preservatives
Flavors
Natural and Artificial Flavorings
Flavor Enhancers
Acids
Sweeteners
Sodium Reduction
Conclusion
Chapter 13: Food Safety and Spoilage�������������������������������������������
Introduction
Food Safety
Hazard Analysis Critical Control Points (HACCP)
The Seven HACCP Principles
Identification of Hazards and Controls
Chemical Hazards
Physical Hazards
Biological Hazards
Process Temperature Control
Receiving Temperatures
Pre-Preparation Temperatures
Cooking/Pasteurization Temperatures
Hot Holding Temperatures
Cooling Temperatures
Cold Display Temperatures
Food Processes
Group I Foods
Group II Foods
Group III Foods
Group IV Foods
Group V Foods
Ingredient HACCP
The HACCP Recipe or Formula
The HACCP Flow Chart
The HACCP Plan
Process Validation
Food Spoilage
Signs of Food Spoilage and Food Industry Consequences
Ubiquitous in Nature
Types of Spoilage Microorganisms
Parameters of Food That Lead to Spoilage
Intrinsic Factors
Extrinsic Factors
Meat
Carcass Spoilage
Frozen Meat
Ground Meat
Cured Meats
Dried Meats
Cooked Meats
Retorted Meat Products
Cooked and Cured Perishable Meats
Cooked and Cured Shelf‐Stable Meat Products
Fish and Seafood
Fish Spoilage
Spoilage Microorganisms
Poultry
Frozen Chicken
Cooked Poultry Products
Dried Poultry Meat
Vegetables
Raw Vegetables
Processed Vegetables
Dried Vegetables
Fermented and Acidified Vegetables
Fruits
Fresh Fruits
Frozen Fruits
Canned Fruits
Dried Fruits
Fruit Juices, Concentrates, and Preserves
Fermented and Acidified Fruits
Spices and Herbs
Cereal Products
Processing and Harvest
Dough-Based Products
Nuts and Legumes
Legumes
Eggs and Egg Products
Shell Eggs
Liquid Eggs
Dried Eggs
Milk and Dairy Products
Pasteurized Milk and Cream
Shelf-Stable Milk
Concentrated Milk Products
Dried Dairy Products
Butter
Fermented Milk
Oil-Based Emulsified Dressings
Sugar, Syrups, and Honey
Conclusion
Chapter 14: Shelf-Life Extension���������������������������������������
What Is Shelf Life?
Shelf-Life Labeling
Changes in Foods During Storage
Biological Changes
Chemical Changes
Color Changes
Flavor Changes
Nutritional Quality
Physical Changes
Other Factors Influencing Shelf Life
Intrinsic Factors
Extrinsic Factors
Analyzing and Predicting Shelf Life
Monitoring Effectiveness of Food Preservation
Conclusion
Chapter 15: Food Packaging���������������������������������
Introduction
Definitions and Vocabulary
What Is Packaging?
Packaging Definitions
The Functions of Packaging
A Brief History of Packaging
The Packaging Supply Industry
Product Distribution
Food and Beverage Distribution
Foodservice Distribution Channels
Retail Grocery Distribution
Food Science, Food Technology, and Culinology®
Microbiological Safety
Package Materials
Paper, Paperboard, and Corrugated Fiberboard
Metal
Glass
Plastics and Plastic Packaging
Package Testing Principles and Protocols
Economics
Environmental Impact
Sustainable Packaging
Further Examples of Current and Future Trends in Food Packaging
Conclusion
Resources
Chapter 16: Developing Nutritious Food Products������������������������������������������������������
Introduction: Overview of Health, Well-Being, and Healthy Eating
Our Changing Understanding of Nutrition, Food, and Health
Basic Nutrition Principles and Rationale
Nutritional Analysis Software
Ingredients: Nutrition and Function
Protein
Lipids
Carbohydrates
Dietary Fiber
Resistant Starches
Water
Micronutrients: Vitamins and Minerals
Health Issues and Trends
Nutrition for Special Groups
Healthy Cooking Approaches and Diets
Allergens
Culinary Nutrition News
Conclusion
Chapter 17: Sensory Evaluation�������������������������������������
Introduction
The Product Development Chef and Sensory Evaluation
Technical Aspects and Applications of Sensory Evaluation
The Five “In” Steps of the Product Development Pathway
Sensory Methods
Qualitative Consumer Research
Quantitative Consumer Research
Preference Tests
Consumer Acceptance Tests
Measuring Consumer Emotion about Foods
Sensory Discrimination Tests
Sensory Descriptive Analysis
Basic Sensory Attributes
Minimizing Errors in Sensory Measurements
Applications of Sensory Analysis in Food Product Development
Step 1: How Do People Think? What Do People Want in a Pizza?
Step 2: What Works in Today’s World of Pizza? Evaluating Many Products to Discover Patterns
Step 3: Nature Reveals Her Secrets: Building and Using the Product Model
Conclusion
Chapter 18: Culinology® Applications in Food Processing—From the Chef’s and Food Scientist’s Perspective���������������������������������������������������������������������������������������������������������������
Introduction: Today’s Case for Processed Foods—Scientific and Culinary Perspectives
Effects of Processing on Nutrient Retention
Traditional Food Processing Technologies
Preparation and Size Reduction
Minimally Processed Fresh foods
Thermal Processing
Chilling and Freezing
Dehydration
Concentrated and Condensed Foods
Osmotic Preserves (Jams, Jelly, Preserves)
Food Extrusion
Specialized Processing Technologies
Cook-Chill and Sous Vide
Irradiation
Other Advanced Technologies
Commercial Meat and Poultry Processing
Marination
Marinade Types
Conclusion
Chapter 19: Commercializing the Culinary Gold Standard
Introduction
When to Launch?
Where to Launch?
Who is going to buy my product?
How am I going to do it (successfully)?
Product Development: The Benefits of Using a Proven Process
The Product Development Process
Identifying a Need
Defining the Vision
Collaborative Marketing
Market Opportunity Assessment
Project and Product Statements
What Is a Gold Standard, and How Is It Developed?
Product and Process Development
Developing Specifications
Scale-Up Testing
Product Launch
Market Testing
Product Rollout
Financial Evaluation: An Ongoing Exercise
Post‐Launch Evaluation
Product Optimization
Improvements Directed by PLE
Competitive Pressures
Impact of Economic and Cultural Change
New Technology and Packaging
Life Cycle: Process Improvements
Conclusion
Index
EULA
What Will You Get?
We provide professional writing services to help you score straight A’s by submitting custom written assignments that mirror your guidelines.
Premium Quality
Get result-oriented writing and never worry about grades anymore. We follow the highest quality standards to make sure that you get perfect assignments.
Experienced Writers
Our writers have experience in dealing with papers of every educational level. You can surely rely on the expertise of our qualified professionals.
On-Time Delivery
Your deadline is our threshold for success and we take it very seriously. We make sure you receive your papers before your predefined time.
24/7 Customer Support
Someone from our customer support team is always here to respond to your questions. So, hit us up if you have got any ambiguity or concern.
Complete Confidentiality
Sit back and relax while we help you out with writing your papers. We have an ultimate policy for keeping your personal and order-related details a secret.
Authentic Sources
We assure you that your document will be thoroughly checked for plagiarism and grammatical errors as we use highly authentic and licit sources.
Moneyback Guarantee
Still reluctant about placing an order? Our 100% Moneyback Guarantee backs you up on rare occasions where you aren’t satisfied with the writing.
Order Tracking
You don’t have to wait for an update for hours; you can track the progress of your order any time you want. We share the status after each step.
From brainstorming your paper's outline to perfecting its grammar, we perform every step carefully to make your paper worthy of A grade.
Preferred Writer
Hire your preferred writer anytime. Simply specify if you want your preferred expert to write your paper and we’ll make that happen.
Grammar Check Report
Get an elaborate and authentic grammar check report with your work to have the grammar goodness sealed in your document.
One Page Summary
You can purchase this feature if you want our writers to sum up your paper in the form of a concise and well-articulated summary.
Plagiarism Report
You don’t have to worry about plagiarism anymore. Get a plagiarism report to certify the uniqueness of your work.
Free Features $66FREE
Most Qualified Writer
$10FREE
Plagiarism Scan Report
$10FREE
Unlimited Revisions
$08FREE
Paper Formatting
$05FREE
Cover Page
$05FREE
Referencing & Bibliography
$10FREE
Dedicated User Area
$08FREE
24/7 Order Tracking
$05FREE
Periodic Email Alerts
$05FREE
Our Services
Join us for the best experience while seeking writing assistance in your college life. A good grade is all you need to boost up your academic excellence and we are all about it.
On-time Delivery
24/7 Order Tracking
Access to Authentic Sources
Academic Writing
We create perfect papers according to the guidelines.
Professional Editing
We seamlessly edit out errors from your papers.
Thorough Proofreading
We thoroughly read your final draft to identify errors.
Thorough Proofreading
We thoroughly read your final draft to identify errors.
Delegate Your Challenging Writing Tasks to Experienced Professionals
Work with ultimate peace of mind because we ensure that your academic work is our responsibility and your grades are a top concern for us!
We Analyze Your Problem and Offer Customized Writing
We understand your guidelines first before delivering any writing service. You can discuss your writing needs and we will have them evaluated by our dedicated team.
Clear elicitation of your requirements.
Customized writing as per your needs.
We Mirror Your Guidelines to Deliver Quality Services
We write your papers in a standardized way. We complete your work in such a way that it turns out to be a perfect description of your guidelines.
Proactive analysis of your writing.
Active communication to understand requirements.
We Handle Your Writing Tasks to Ensure Excellent Grades
We promise you excellent grades and academic excellence that you always longed for. Our writers stay in touch with you via email.
Thorough research and analysis for every order.
Deliverance of reliable writing service to improve your grades.
